##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_50330665-1.341000000356c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2835060 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67666786593582 33.0 33.0 33.0 33.0 33.0 2 32.67513773958929 33.0 33.0 33.0 33.0 33.0 3 32.72292402982653 33.0 33.0 33.0 33.0 33.0 4 36.64031731250838 37.0 37.0 37.0 37.0 37.0 5 36.624751857103554 37.0 37.0 37.0 37.0 37.0 6 36.5184458177252 37.0 37.0 37.0 37.0 37.0 7 36.33116583070552 37.0 37.0 37.0 37.0 37.0 8 36.287698673043955 37.0 37.0 37.0 37.0 37.0 9 36.385753035209135 37.0 37.0 37.0 37.0 37.0 10-11 36.27081067067364 37.0 37.0 37.0 35.0 37.0 12-13 36.26137400972114 37.0 37.0 37.0 35.0 37.0 14-15 38.04474684839121 40.0 37.0 40.0 35.0 40.0 16-17 38.054503079299906 40.0 37.0 40.0 37.0 40.0 18-19 38.005529512602905 40.0 37.0 40.0 35.0 40.0 20-21 38.027753028154606 40.0 37.0 40.0 33.0 40.0 22-23 37.70844126755695 40.0 37.0 40.0 33.0 40.0 24-25 37.8941357502134 40.0 37.0 40.0 33.0 40.0 26-27 37.82739060196256 40.0 37.0 40.0 33.0 40.0 28-29 37.54468847220164 40.0 37.0 40.0 33.0 40.0 30-31 37.45023085931162 40.0 37.0 40.0 33.0 40.0 32-33 37.40920862345065 38.5 37.0 40.0 33.0 40.0 34-35 37.23119087426721 40.0 37.0 40.0 33.0 40.0 36-37 37.206722609045315 38.5 37.0 40.0 33.0 40.0 38-39 37.17745709085522 40.0 37.0 40.0 33.0 40.0 40-41 37.11328560947564 40.0 37.0 40.0 33.0 40.0 42-43 36.41825763828631 37.0 37.0 40.0 33.0 40.0 44-45 36.23243740873209 37.0 37.0 40.0 33.0 40.0 46-47 36.64603147728796 37.0 37.0 40.0 33.0 40.0 48-49 36.30176151474748 37.0 37.0 40.0 33.0 40.0 50-51 36.29032507248524 37.0 37.0 40.0 33.0 40.0 52-53 36.30129609249893 37.0 37.0 40.0 33.0 40.0 54-55 35.90133859600855 37.0 35.0 40.0 30.0 40.0 56-57 35.785597306582574 37.0 37.0 40.0 30.0 40.0 58-59 35.673921363216294 37.0 33.0 40.0 27.0 40.0 60-61 35.38647859304565 37.0 33.0 40.0 27.0 40.0 62-63 34.98233423631246 37.0 33.0 37.0 27.0 40.0 64-65 34.664397049797884 37.0 33.0 37.0 27.0 40.0 66-67 33.79202503650716 37.0 33.0 37.0 27.0 40.0 68-69 33.57614389113458 37.0 33.0 37.0 27.0 37.0 70-71 33.12599345340134 37.0 33.0 37.0 27.0 37.0 72-73 31.74434297686821 33.0 30.0 37.0 18.5 37.0 74-75 30.443444583183425 33.0 27.0 37.0 15.0 37.0 76-77 27.806838303245787 30.0 24.5 33.0 15.0 35.0 78-79 29.716512701671217 33.0 27.0 37.0 15.0 37.0 80-81 29.97798476928178 33.0 27.0 37.0 10.5 37.0 82-83 29.80403465887847 33.0 27.0 37.0 6.0 37.0 84-85 29.627063801118847 33.0 27.0 37.0 6.0 37.0 86-87 29.42866376725713 33.0 27.0 37.0 6.0 37.0 88-89 29.310284262061472 33.0 27.0 37.0 6.0 37.0 90-91 29.16333093479503 33.0 27.0 37.0 6.0 37.0 92-93 28.944368373156124 33.0 27.0 37.0 6.0 37.0 94-95 28.80929169047569 33.0 27.0 37.0 6.0 37.0 96-97 28.652954434826775 33.0 27.0 37.0 6.0 37.0 98-99 28.352459030849435 33.0 22.0 37.0 6.0 37.0 100-101 26.853847184892032 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11478938968805608 2306 2 0.13457556611587762 2306 3 0.14711958506499911 2306 4 0.1309354004862513 2306 5 0.14340256207338342 2306 6 0.1466002532457722 2306 7 0.1618508824319207 2306 8 0.22608546222777903 2306 9 0.24213276065245282 2306 10-11 0.24782282532426336 2306 12-13 0.2620995539295734 2306 14-15 0.5179432071612169 2306 16-17 0.4910018264733438 2306 18-19 0.5446085625144335 2306 20-21 0.5269123926898303 2306 22-23 0.5409753299351223 2306 24-25 0.583526333061883 2306 26-27 0.5901692000375576 2306 28-29 0.5112531562705627 2306 30-31 0.5411608809580954 2306 32-33 0.6051414963714379 2306 34-35 0.6979796584330344 2306 36-37 0.6379509709423417 2306 38-39 0.7325476849275177 2306 40-41 0.7624916873418002 2306 42-43 0.820700121133541 2306 44-45 0.7242121258227598 2306 46-47 0.7980060543323688 2306 48-49 0.9085728481275339 2306 50-51 0.8430748758058471 2306 52-53 0.7814849202223613 2306 54-55 0.8615242852693186 2306 56-57 0.8793995692595544 2306 58-59 0.7618341415879257 2306 60-61 0.761719995592216 2306 62-63 0.8262109732512428 2306 64-65 0.6658021433059105 2306 66-67 0.5879614083210143 2306 68-69 0.7219354167086678 2306 70-71 0.7015417230051142 2306 72-73 0.11480690095557122 2306 74-75 0.1808961619773939 2306 76-77 0.10664701928370945 2306 78-79 0.6336186685770251 2306 80-81 0.7709916337675686 2306 82-83 0.829410948863913 2306 84-85 0.817596586573174 2306 86-87 0.6892271037024251 2306 88-89 0.7821828522185896 2306 90-91 0.909931318288816 2306 92-93 0.917936929570569 2306 94-95 0.7358372072259698 2306 96-97 0.8422322205660464 2306 98-99 0.8006836534345858 2306 100-101 0.5833012923614156 2307 1 0.14045820121339858 2307 2 0.02588248214498634 2307 3 0.08577307999747319 2307 4 0.16380125162886117 2307 5 0.18185452860403473 2307 6 0.19714826717034128 2307 7 0.15680941492294664 2307 8 0.19235204000188588 2307 9 0.24813908881800018 2307 10-11 0.27524046036240435 2307 12-13 0.2350041906029645 2307 14-15 0.5359270110532393 2307 16-17 0.44621224775022483 2307 18-19 0.4746434608055665 2307 20-21 0.4843828420677596 2307 22-23 0.4635735478854812 2307 24-25 0.43909985292479803 2307 26-27 0.5262434354566139 2307 28-29 0.5237515003933382 2307 30-31 0.5467780508304756 2307 32-33 0.48312430130801687 2307 34-35 0.4732075179972739 2307 36-37 0.537981916844565 2307 38-39 0.578875763610462 2307 40-41 0.6220988155403546 2307 42-43 0.6516095397631929 2307 44-45 0.5397416482134716 2307 46-47 0.5271864113937852 2307 48-49 0.4963507369003679 2307 50-51 0.6680121696355599 2307 52-53 0.5465045410101936 2307 54-55 0.5696994325609523 2307 56-57 0.5781608645138689 2307 58-59 0.4798903697493486 2307 60-61 0.5107726166558422 2307 62-63 0.4077104270832095 2307 64-65 0.4080182173675979 2307 66-67 0.5172372090598074 2307 68-69 0.5794643918455336 2307 70-71 0.3141016331859916 2307 72-73 -0.07396929176464795 2307 74-75 -0.18963562977948456 2307 76-77 -0.12935392320903105 2307 78-79 0.41116182453785655 2307 80-81 0.3219972127165356 2307 82-83 0.4036658041130998 2307 84-85 0.25186303839203106 2307 86-87 0.26775531659286145 2307 88-89 0.24339851583988903 2307 90-91 0.3351191110533378 2307 92-93 0.29160685297674505 2307 94-95 0.5088148110793327 2307 96-97 0.43165556268004934 2307 98-99 0.42937387083768996 2307 100-101 0.1670068089842509 2201 1 0.1341229013511338 2201 2 0.14712678821238967 2201 3 0.14470089249957852 2201 4 0.17752442779464417 2201 5 0.19716877177875602 2201 6 0.16653724108985557 2201 7 0.19413966837066 2201 8 0.16636838201998216 2201 9 0.22672486258768032 2201 10-11 0.2597873286845953 2201 12-13 0.27445031118914187 2201 14-15 0.4097821233604648 2201 16-17 0.35380883379145445 2201 18-19 0.3929774178144214 2201 20-21 0.32997900509429456 2201 22-23 0.439247127363636 2201 24-25 0.47055065841860255 2201 26-27 0.5693788315090771 2201 28-29 0.42619194996352405 2201 30-31 0.5194638409889905 2201 32-33 0.4792481215989923 2201 34-35 0.5210958770375456 2201 36-37 0.5517899142032974 2201 38-39 0.5547730527846539 2201 40-41 0.5657847854602807 2201 42-43 0.6595007876843297 2201 44-45 0.5808789347368801 2201 46-47 0.6105453068254292 2201 48-49 0.7555884096528587 2201 50-51 0.6754753433489711 2201 52-53 0.603215891415168 2201 54-55 0.6050775088916893 2201 56-57 0.5406827940164476 2201 58-59 0.6164992164708067 2201 60-61 0.6490922721171231 2201 62-63 0.5034489508254083 2201 64-65 0.5697136015470861 2201 66-67 0.6888596489974788 2201 68-69 0.6834605315387918 2201 70-71 0.6956733907247568 2201 72-73 0.227847627791526 2201 74-75 0.053986411746588914 2201 76-77 0.01436720534339031 2201 78-79 0.5421685042812037 2201 80-81 0.5218091576833572 2201 82-83 0.40777739983485617 2201 84-85 0.39537444530478894 2201 86-87 0.5075144720237823 2201 88-89 0.558894167240858 2201 90-91 0.5874023388380216 2201 92-93 0.5763830460587478 2201 94-95 0.5917788663379113 2201 96-97 0.6143283687476533 2201 98-99 0.5243339245200822 2201 100-101 0.45366434604665073 1101 1 0.04613103721348466 1101 2 0.08517969863141417 1101 3 0.0671638299865478 1101 4 0.16261037968416758 1101 5 0.17913731177567627 1101 6 0.08710478486245421 1101 7 0.14910854432697818 1101 8 0.1477839091712383 1101 9 0.22857527962346325 1101 10-11 0.2456305093916029 1101 12-13 0.2962618152038061 1101 14-15 0.446808035936364 1101 16-17 0.41253435496098945 1101 18-19 0.3348532548677383 1101 20-21 0.42380727612339086 1101 22-23 0.540524410408068 1101 24-25 0.37310486032382073 1101 26-27 0.4700437397757753 1101 28-29 0.4528199646073432 1101 30-31 0.4986409134442411 1101 32-33 0.48289596695205006 1101 34-35 0.47224137113003195 1101 36-37 0.5371682145036587 1101 38-39 0.6741259530975867 1101 40-41 0.5747337788112006 1101 42-43 0.6543307457941054 1101 44-45 0.6907693427773367 1101 46-47 0.6476565294171976 1101 48-49 0.5916352145332837 1101 50-51 0.6679716127428819 1101 52-53 0.5202709751000185 1101 54-55 0.6009356484413999 1101 56-57 0.6035992493807356 1101 58-59 0.6050725908233616 1101 60-61 0.451448995447727 1101 62-63 0.5990436945630435 1101 64-65 0.5962753347698353 1101 66-67 0.547618310877553 1101 68-69 0.679559942744909 1101 70-71 0.5601448191531162 1101 72-73 0.2411764755964576 1101 74-75 0.2062557765283941 1101 76-77 0.2518057379746992 1101 78-79 0.6162116800717357 1101 80-81 0.709445851733598 1101 82-83 0.43697991962141103 1101 84-85 0.4887452106457957 1101 86-87 0.5210186742476672 1101 88-89 0.37213606620357353 1101 90-91 0.5778512588969846 1101 92-93 0.5605680076419297 1101 94-95 0.4338850495386666 1101 96-97 0.5403827031246777 1101 98-99 0.6847983314880075 1101 100-101 0.6577319130359562 2304 1 0.09982197020603678 2304 2 0.09115766883070364 2304 3 0.07749994189833131 2304 4 0.11447504852904444 2304 5 0.18404544873965278 2304 6 0.16300346310773506 2304 7 0.1667193889470724 2304 8 0.19135316342033093 2304 9 0.1716881761040554 2304 10-11 0.1786315573983046 2304 12-13 0.16147365751295695 2304 14-15 0.24489046691204663 2304 16-17 0.3341029660771895 2304 18-19 0.2914067956808495 2304 20-21 0.25963052787660246 2304 22-23 0.37708684844820795 2304 24-25 0.3100808361446923 2304 26-27 0.282485778673653 2304 28-29 0.4350408391014611 2304 30-31 0.43599552125813545 2304 32-33 0.4354743300288959 2304 34-35 0.5003754360131367 2304 36-37 0.5154436519923067 2304 38-39 0.5513443866857841 2304 40-41 0.476425501557614 2304 42-43 0.696674631949584 2304 44-45 0.6821806862419493 2304 46-47 0.5338303385800032 2304 48-49 0.5803745574380841 2304 50-51 0.4929431632763013 2304 52-53 0.5086209393611583 2304 54-55 0.502238409080995 2304 56-57 0.48485359177735887 2304 58-59 0.41373830667524913 2304 60-61 0.4723781391979216 2304 62-63 0.43652026436544844 2304 64-65 0.4062199853537294 2304 66-67 0.3320409158323443 2304 68-69 0.49738622022176315 2304 70-71 0.3033096994320985 2304 72-73 0.3257656119822805 2304 74-75 0.5041019478050117 2304 76-77 0.3640542538539151 2304 78-79 0.841569610183516 2304 80-81 0.800442574421691 2304 82-83 0.9248099625913504 2304 84-85 0.8904654245124775 2304 86-87 0.8846073557752554 2304 88-89 1.0338568298988875 2304 90-91 0.9304482828245284 2304 92-93 0.8715538337261322 2304 94-95 0.8298773819284051 2304 96-97 0.7577595373446755 2304 98-99 0.9292582248212504 2304 100-101 0.7433064922920849 2202 1 0.049109272079384425 2202 2 0.0752088492235643 2202 3 0.05539600210652651 2202 4 0.10403773249612414 2202 5 0.13697991140560362 2202 6 0.14339461037579326 2202 7 0.1617528141030462 2202 8 0.0857399684216631 2202 9 0.09964387811815101 2202 10-11 0.13727168131595846 2202 12-13 0.20355331150466327 2202 14-15 0.18166800726680066 2202 16-17 0.12574496077379393 2202 18-19 0.12150595554663823 2202 20-21 0.14817543944367628 2202 22-23 0.26168424480447783 2202 24-25 0.21486741287666433 2202 26-27 0.21395562738084095 2202 28-29 0.25016089505319883 2202 30-31 0.16890642755613783 2202 32-33 0.24379993996290494 2202 34-35 0.29542859874516836 2202 36-37 0.22642546767767868 2202 38-39 0.2801951448661555 2202 40-41 0.24013989883211195 2202 42-43 0.3737775419548228 2202 44-45 0.23433937635342517 2202 46-47 0.18157896767662862 2202 48-49 0.2651129058320407 2202 50-51 0.3197984954253812 2202 52-53 0.31795488198488187 2202 54-55 0.31638874933964445 2202 56-57 0.4842142659443027 2202 58-59 0.42545427437428174 2202 60-61 0.3152992146383724 2202 62-63 0.4126281430909202 2202 64-65 0.44690620938380476 2202 66-67 0.4161347894603722 2202 68-69 0.5249512469533073 2202 70-71 0.4448045204637694 2202 72-73 0.1615221618539877 2202 74-75 0.06391109402612472 2202 76-77 0.03377702672590388 2202 78-79 0.22616593384544004 2202 80-81 0.24957433561418796 2202 82-83 0.28962151164780536 2202 84-85 0.18139085938276622 2202 86-87 0.2718854153140846 2202 88-89 0.2773369936349539 2202 90-91 0.2881189055879574 2202 92-93 0.2811782619800418 2202 94-95 0.34025248168897804 2202 96-97 0.17436398773899953 2202 98-99 0.26876598683757535 2202 100-101 0.21257487183709145 1102 1 0.08862548626122191 1102 2 0.07298133637449666 1102 3 0.08621929630511005 1102 4 0.09249911375844988 1102 5 0.08448690218560273 1102 6 0.07728959029752502 1102 7 0.11646601330876649 1102 8 0.10817688110248014 1102 9 0.09424624663868997 1102 10-11 0.09406806484325614 1102 12-13 0.1379472679285172 1102 14-15 0.22710211682365156 1102 16-17 0.19481744349694452 1102 18-19 0.25989572601744726 1102 20-21 0.3088416156487952 1102 22-23 0.3772313648755983 1102 24-25 0.31804442862252813 1102 26-27 0.23699539904374234 1102 28-29 0.1853897430475797 1102 30-31 0.3041192674306572 1102 32-33 0.12914816765967174 1102 34-35 0.13597239798290417 1102 36-37 0.22427033196628798 1102 38-39 0.26092579632850743 1102 40-41 0.3368319230418422 1102 42-43 0.1488187543485182 1102 44-45 0.21532036756258321 1102 46-47 0.2342899598053947 1102 48-49 0.23143241768656253 1102 50-51 0.18447240661777897 1102 52-53 0.2894787357864672 1102 54-55 0.28535641293752434 1102 56-57 0.4306092032912474 1102 58-59 0.308721020740947 1102 60-61 0.2436773536774055 1102 62-63 0.15882664037124528 1102 64-65 0.3828417988402393 1102 66-67 0.3560996567462382 1102 68-69 0.3774368481603858 1102 70-71 0.2681591752503678 1102 72-73 -0.05330127198990198 1102 74-75 -0.16569984054153863 1102 76-77 0.03828016193320849 1102 78-79 0.1814747543194848 1102 80-81 0.128420541860077 1102 82-83 0.17746036437651114 1102 84-85 0.1906108552274688 1102 86-87 0.11330327492627745 1102 88-89 0.016410600332477543 1102 90-91 0.2316340843509721 1102 92-93 0.13880349145814463 1102 94-95 0.23788736712874226 1102 96-97 0.1613471724011788 1102 98-99 0.06990426633934632 1102 100-101 -0.07296358000742131 2305 1 0.03942484057819229 2305 2 0.05488238998495376 2305 3 0.08321888405485112 2305 4 0.10853865393598028 2305 5 0.10773871329274698 2305 6 0.052089343158236545 2305 7 0.11681974723127553 2305 8 0.11678405510055967 2305 9 0.09138817143622902 2305 10-11 0.07680205377049276 2305 12-13 0.047319951656653814 2305 14-15 0.11854718579385093 2305 16-17 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-0.11266363645060551 1213 70-71 0.037335295618980524 1213 72-73 0.5708222004208459 1213 74-75 0.4789464057194657 1213 76-77 -1.919357323658467 1213 78-79 -1.9327440842929775 1213 80-81 -1.6699555916116573 1213 82-83 -1.299032317321899 1213 84-85 -0.9377629442496378 1213 86-87 -0.8436544859129746 1213 88-89 -0.8626357581240569 1213 90-91 -1.8189752110897501 1213 92-93 -1.1444400649764113 1213 94-95 -0.7275515978879525 1213 96-97 -0.8260594934055945 1213 98-99 -2.34910151336841 1213 100-101 -1.2288431991158753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 20.0 10 20.0 11 18.0 12 39.0 13 80.0 14 255.0 15 726.0 16 1588.0 17 2525.0 18 3421.0 19 4559.0 20 6410.0 21 8799.0 22 12091.0 23 16507.0 24 22660.0 25 30174.0 26 42069.0 27 64084.0 28 111807.0 29 185160.0 30 147387.0 31 76916.0 32 88826.0 33 126898.0 34 195764.0 35 334358.0 36 569110.0 37 675755.0 38 107003.0 39 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.334860260544545 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20.897921190013825 23.03295574572597 31.337613901930457 24.73150916232975 20-21 27.540775151143187 23.9816264911501 31.30231811672416 17.175280240982556 22-23 20.127157802656733 38.267091349036704 24.020020740301792 17.585730108004768 24-25 20.559176878090764 22.04995661467482 32.36462367639486 25.026242830839557 26-27 19.118325538083848 22.194415638469735 25.283027519699758 33.404231303746656 28-29 26.136995336959355 30.732524179382448 23.672338504299734 19.458141979358462 30-31 17.267906146607125 24.47175721148759 31.308049917814788 26.952286724090495 32-33 17.761193766622224 30.774868962208913 23.972614336204526 27.49132293496434 34-35 18.846796893187445 30.34796441697883 31.667213392309158 19.138025297524567 36-37 17.629541813849055 32.391806944439786 32.16235849723789 17.816292744473262 38-39 17.625619256063814 24.998051184192192 30.221600639141037 27.15472892060295 40-41 26.351586581857084 23.90807310047029 22.58564818978948 27.154692127883145 42-43 19.427596179436065 31.443497970830254 30.419151284829262 18.709754564904422 44-45 17.928669864972065 25.120271544382216 30.15596236106472 26.795096229581 46-47 26.191260625972472 24.237129341000106 23.705791033741022 25.8658189992864 48-49 18.66958005080844 30.963131075478262 31.61195811442975 18.755330759283545 50-51 25.36328673764638 23.79650051722359 29.808605224796043 21.031607520333985 52-53 17.360845208488136 24.424702344366057 23.227871576367164 34.986580870778646 54-55 25.75316798887434 24.393117440758193 31.382890337611887 18.47082423275559 56-57 18.90133932374672 24.217590148554468 30.09916389496575 26.781906632733065 58-59 17.94320757815577 23.94213402607959 30.3614497786306 27.75320861713404 60-61 24.567910968079445 23.564490448808886 31.86755272455392 20.000045858557744 62-63 17.63016123157892 23.342625262465397 31.837447385663854 27.189766120291825 64-65 26.174987917635313 24.975570693298433 30.34010533705388 18.509336052012376 66-67 19.3184692992221 38.79290969271877 22.975383700182665 18.91323730787646 68-69 18.476436827438157 37.31272004138136 24.608291939031645 19.60255119214883 70-71 17.989974253736808 39.50875501741618 23.604205146535634 18.897065582311384 72-73 19.788827622828844 39.09337012237236 23.074525148830656 18.043277105968134 74-75 21.095236238580263 36.846168597956265 23.260652908260848 18.797942255202628 76-77 20.528340393820642 34.918238074974546 23.763709024650662 20.78971250655415 78-79 20.26912704986855 34.82696858643915 24.33085135957033 20.573053004121963 80-81 19.59720888199724 34.88839302248718 24.492723615190584 21.021674480324997 82-83 19.19851784441952 34.12079462163059 24.67291697530211 22.00777055864779 84-85 20.04345117615893 32.99534446644792 24.588734159086755 22.3724701983064 86-87 18.90923883600443 33.28529717937984 25.39948245145623 22.4059815331595 88-89 18.536045893575352 33.593150892999375 25.50188380079501 22.36891941263026 90-91 19.416705534853296 32.36755591903092 24.802865967203953 23.412872578911827 92-93 19.9061903898254 32.26768660123823 24.02873567679756 23.797387332138804 94-95 20.298818434017104 33.331804369015536 23.522998416639837 22.846378780327523 96-97 19.81605852705684 34.10614556678856 22.823689146638024 23.254106759516574 98-99 19.82371361985016 32.173270054481236 24.471882766981256 23.531133558687348 100-101 20.713512025939476 33.1923162414573 23.712481785561817 22.381689947041412 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1205.0 1 726.0 2 335.5 3 546.0 4 493.0 5 283.5 6 267.5 7 326.0 8 359.5 9 361.5 10 363.0 11 335.5 12 290.0 13 289.5 14 295.0 15 278.0 16 292.0 17 309.5 18 315.5 19 319.5 20 330.0 21 375.0 22 430.5 23 463.5 24 538.5 25 658.0 26 808.0 27 1085.5 28 1398.5 29 1848.5 30 2480.0 31 3530.5 32 5940.5 33 10607.5 34 18564.0 35 32722.5 36 53500.5 37 81439.0 38 118979.0 39 151208.0 40 169747.0 41 173091.5 42 187653.5 43 216997.5 44 208339.0 45 178871.0 46 171914.5 47 166149.0 48 143593.0 49 118910.5 50 97430.0 51 84715.5 52 80487.0 53 80921.5 54 72186.0 55 46927.5 56 28240.5 57 23040.5 58 22084.0 59 19429.5 60 15021.5 61 11242.0 62 7619.5 63 4810.0 64 3309.0 65 2262.0 66 1515.5 67 1151.0 68 869.0 69 641.0 70 487.0 71 352.0 72 237.5 73 161.5 74 119.0 75 73.5 76 43.0 77 31.5 78 21.5 79 16.5 80 13.0 81 9.5 82 8.0 83 7.0 84 6.0 85 3.5 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16779186331153487 2 0.006313799355216468 3 0.0016225406164243439 4 0.0 5 0.0012698143954625299 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.818155524045346E-5 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.7636311048090695E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.7636311048090695E-5 38-39 1.763631104809069E-4 40-41 1.0581786628854416E-4 42-43 0.0011816328402220765 44-45 5.996345756350836E-4 46-47 0.005043984959753938 48-49 0.003456716965425776 50-51 0.0074954321954385445 52-53 0.004038715230012768 54-55 0.01880030757726468 56-57 0.010881603916671957 58-59 0.019205942731370766 60-61 0.009047427567670525 62-63 0.005731801090629475 64-65 0.012345417733663485 66-67 0.0310751800667358 68-69 0.03294462903783341 70-71 0.037265525244615634 72-73 0.031128088999880073 74-75 0.034796441697882936 76-77 0.034496624410065395 78-79 0.017213039582936514 80-81 0.002363265680444153 82-83 0.0 84-85 0.005431983802811934 86-87 0.025978286173837593 88-89 0.02693064697043449 90-91 0.024479199734749883 92-93 0.03604861978229738 94-95 0.0316571783313228 96-97 0.03357953623556468 98-99 0.04051060647746432 100-101 0.027159919014059664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2835060.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.61484282865984 #Duplication Level Percentage of deduplicated Percentage of total 1 76.53744475127486 18.839571730598667 2 9.319280412989933 4.587852452839107 3 4.1698469212822005 3.0792037976079745 4 2.040102330353689 2.0086719286415486 5 1.5241491539790375 1.8758345936314438 6 0.912452783493811 1.3475929112564007 7 0.7637713971989155 1.3160079029354061 8 0.4727277867572201 0.9308896137415358 9 0.38988953384787867 0.8637362636584486 >10 3.1036641463017314 15.186226369253733 >50 0.3981970329040107 6.941266455089886 >100 0.32699656249649517 16.48625574674367 >500 0.02841787051957392 4.680167142560494 >1k 0.01175737393887001 5.29502383632286 >5k 5.786411830483631E-4 1.01778073250624 >10k+ 7.233014787449505E-4 15.54391852261261 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 376685 13.286667654300086 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 19905 0.7021015428244906 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 18575 0.6551889554365692 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10888 0.38404830938322293 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 10477 0.3695512617016924 TruSeq Adapter, Index 27 (97% over 39bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 8494 0.2996056520849647 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7566 0.2668726587797084 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7353 0.25935959027322175 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5170 0.18235945623725777 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4830 0.1703667647245561 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4752 0.16761550020105395 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 3997 0.140984670518437 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG 3743 0.13202542450600693 TruSeq Adapter, Index 27 (97% over 38bp) TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3743 0.13202542450600693 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3630 0.12803961820913842 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3556 0.12542944417402102 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3480 0.12274872489471123 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3346 0.11802219353382291 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC 3263 0.11509456589983987 TruSeq Adapter, Index 27 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006419617221505012 0.0 0.0 2 0.007442523262294273 0.0 0.0 3 0.008183248326314082 0.0 0.0 4 0.008712337657756803 0.0 0.0 5 0.010617059250950597 0.0 0.0 6 0.011498874803355132 0.0 0.0 7 0.01347414164074129 0.0 0.0 8 0.015061409635069451 0.0 0.0 9 0.016966131228263247 0.0 0.0 10-11 0.026154649284318498 0.0 0.0 12-13 0.03361480885766086 0.0 0.0 14-15 0.04211551078284058 0.0 0.0 16-17 0.05072203057430883 0.0 0.0 18-19 0.06022800222922972 0.0 0.0 20-21 0.06536016874422411 0.0 0.0 22-23 0.07098615196856503 0.0 0.0 24-25 0.07701777034701206 0.0 0.0 26-27 0.08299647979231481 0.0 0.0 28-29 0.09112681918548461 0.0 0.0 30-31 0.16867367886393939 0.0 0.0 32-33 0.1817421853505746 0.0 0.0 34-35 0.19123052069444738 0.0 0.0 36-37 0.2020239430558789 0.0 0.0 38-39 0.21369918096971494 0.0 0.0 40-41 0.2277553208750432 0.0 0.0 42-43 0.24394545441719045 0.0 0.0 44-45 0.2608057677791652 0.0 0.0 46-47 0.2793062580686123 0.0 1.7636311048090695E-5 48-49 0.2995174705297242 0.0 3.527262209618139E-5 50-51 0.3221272212933765 0.0 3.527262209618139E-5 52-53 0.34618314956297225 0.0 3.527262209618139E-5 54-55 0.373942703152667 0.0 3.527262209618139E-5 56-57 0.40466515699844097 0.0 3.527262209618139E-5 58-59 0.43665742523967743 0.0 3.527262209618139E-5 60-61 0.47148913955965654 0.0 3.527262209618139E-5 62-63 0.5084195748943585 0.0 3.527262209618139E-5 64-65 0.549547452258506 0.0 3.527262209618139E-5 66-67 0.593497139390348 0.0 3.527262209618139E-5 68-69 0.6412033607754333 0.0 3.527262209618139E-5 70-71 0.6930012063236757 0.0 3.527262209618139E-5 72-73 0.7501604904305377 0.0 3.527262209618139E-5 74-75 0.8119581243430474 0.0 3.527262209618139E-5 76-77 0.8831559120441895 0.0 3.527262209618139E-5 78-79 0.9653234852172441 0.0 3.527262209618139E-5 80-81 1.0561857597370072 0.0 3.527262209618139E-5 82-83 1.1586174543043182 0.0 3.527262209618139E-5 84-85 1.2790205498296332 0.0 3.527262209618139E-5 86-87 1.4086121634110036 0.0 3.527262209618139E-5 88-89 1.5533886408047801 0.0 3.527262209618139E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 49160 0.0 83.29319 1 TCGGAAG 49140 0.0 83.15554 3 CGGAAGA 49355 0.0 82.705246 4 GAAGAGC 49430 0.0 82.62779 6 ATCGGAA 50130 0.0 81.50961 2 GGAAGAG 51380 0.0 79.52883 5 GTCGCAC 20 0.0020616245 71.360985 1 AGAGCAC 57565 0.0 70.78596 8 GAGCACA 57565 0.0 70.7282 9 AAGAGCA 57690 0.0 70.51733 7 TCGGACC 25 0.005023728 56.995144 2 TTGTGTT 23420 0.0 51.697613 1 TTCCCTA 575 0.0 49.55662 8 CGAGGGG 430 0.0 47.57399 1 AACGGTG 60 1.4771413E-6 47.573986 1 TCGAGCG 40 5.391791E-4 47.495953 2 ATCGGAC 175 0.0 46.138924 2 GCCGTCT 44770 0.0 45.66545 50-51 ATGCCGT 44595 0.0 45.657036 48-49 CTGCTTG 44925 0.0 45.575817 58-59 >>END_MODULE