##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_50220371-2.34100000035905.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3348763 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68714089351799 33.0 33.0 33.0 33.0 33.0 2 32.722746279745685 33.0 33.0 33.0 33.0 33.0 3 32.74792363628002 33.0 33.0 33.0 33.0 33.0 4 36.692159761679164 37.0 37.0 37.0 37.0 37.0 5 36.64602750329002 37.0 37.0 37.0 37.0 37.0 6 36.520993572850635 37.0 37.0 37.0 37.0 37.0 7 36.479718331813864 37.0 37.0 37.0 37.0 37.0 8 36.40749255769966 37.0 37.0 37.0 37.0 37.0 9 36.4078977819571 37.0 37.0 37.0 37.0 37.0 10-11 36.35640862013824 37.0 37.0 37.0 37.0 37.0 12-13 36.35236996467054 37.0 37.0 37.0 37.0 37.0 14-15 38.222775245665346 40.0 37.0 40.0 37.0 40.0 16-17 38.07546965849778 40.0 37.0 40.0 37.0 40.0 18-19 37.88491362332897 40.0 37.0 40.0 33.0 40.0 20-21 37.75856144492757 40.0 37.0 40.0 33.0 40.0 22-23 37.61062099646944 40.0 37.0 40.0 33.0 40.0 24-25 37.53943187379937 40.0 37.0 40.0 33.0 40.0 26-27 37.41103998700416 40.0 37.0 40.0 33.0 40.0 28-29 37.257951667526186 40.0 37.0 40.0 33.0 40.0 30-31 37.165548144195334 40.0 37.0 40.0 33.0 40.0 32-33 37.02364798583835 37.0 37.0 40.0 33.0 40.0 34-35 36.90986343315427 37.0 37.0 40.0 33.0 40.0 36-37 36.807899215322195 37.0 37.0 40.0 33.0 40.0 38-39 36.69453855050357 37.0 37.0 40.0 33.0 40.0 40-41 36.564343460555435 37.0 37.0 40.0 33.0 40.0 42-43 36.437577845909075 37.0 37.0 40.0 33.0 40.0 44-45 36.304138274341895 37.0 37.0 40.0 33.0 40.0 46-47 36.15333348463298 37.0 37.0 40.0 33.0 40.0 48-49 35.9216040370728 37.0 37.0 40.0 30.0 40.0 50-51 35.73713816116578 37.0 37.0 40.0 27.0 40.0 52-53 35.56644856623177 37.0 37.0 40.0 27.0 40.0 54-55 35.378988151744394 37.0 37.0 40.0 27.0 40.0 56-57 35.14333426999761 37.0 33.0 40.0 27.0 40.0 58-59 34.909780118808044 37.0 33.0 40.0 27.0 40.0 60-61 34.639896732017164 37.0 33.0 40.0 27.0 40.0 62-63 34.371919272877776 37.0 33.0 37.0 27.0 40.0 64-65 34.06349389311815 37.0 33.0 37.0 27.0 40.0 66-67 33.748771710628674 37.0 33.0 37.0 27.0 40.0 68-69 33.46150832411848 37.0 33.0 37.0 22.0 40.0 70-71 33.18034495125514 37.0 33.0 37.0 22.0 40.0 72-73 32.85454972477898 37.0 33.0 37.0 22.0 37.0 74-75 32.47706048472227 37.0 33.0 37.0 22.0 37.0 76-77 29.636662552709762 33.0 27.0 35.0 18.5 37.0 78-79 32.13226734767435 35.0 33.0 37.0 22.0 37.0 80-81 32.526640583403484 37.0 33.0 37.0 22.0 37.0 82-83 32.42433071555078 37.0 33.0 37.0 22.0 37.0 84-85 32.27118386699806 37.0 33.0 37.0 22.0 37.0 86-87 32.060364677942275 35.0 33.0 37.0 22.0 37.0 88-89 31.863378507227893 33.0 33.0 37.0 22.0 37.0 90-91 31.66321190839722 33.0 33.0 37.0 15.0 37.0 92-93 31.463710181938822 33.0 33.0 37.0 15.0 37.0 94-95 31.34485644400634 33.0 33.0 37.0 15.0 37.0 96-97 31.176083228344318 33.0 33.0 37.0 15.0 37.0 98-99 30.806953343667494 33.0 30.0 37.0 15.0 37.0 100-101 29.242407868218802 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08853195952999471 2306 2 0.10687618258291565 2306 3 0.13684933325221493 2306 4 0.14218155156446954 2306 5 0.17854147443443935 2306 6 0.15923395147287067 2306 7 0.21872361800694762 2306 8 0.2076838678563604 2306 9 0.1932484736308666 2306 10-11 0.22282054541769014 2306 12-13 0.23256347466665517 2306 14-15 0.4026891173189924 2306 16-17 0.4997581767190198 2306 18-19 0.6436027227279837 2306 20-21 0.5692497470348172 2306 22-23 0.6983650365258498 2306 24-25 0.7023822752335249 2306 26-27 0.6054918095948167 2306 28-29 0.6249731148463198 2306 30-31 0.6526833116504065 2306 32-33 0.6696029079892156 2306 34-35 0.6461725088667478 2306 36-37 0.5962305242952652 2306 38-39 0.7300812388329021 2306 40-41 0.8609809638497481 2306 42-43 0.86010383478866 2306 44-45 0.8271183711814913 2306 46-47 0.7310694809113443 2306 48-49 0.8953640407131545 2306 50-51 0.9152308462400427 2306 52-53 0.8664634110162694 2306 54-55 0.9481268566744845 2306 56-57 0.9511033716126249 2306 58-59 0.878901764953973 2306 60-61 0.9649091512140515 2306 62-63 1.063269724317962 2306 64-65 1.0204172192573537 2306 66-67 1.0013844107526992 2306 68-69 1.058195955045285 2306 70-71 0.8427557611804417 2306 72-73 0.857870167554033 2306 74-75 0.9982510993707905 2306 76-77 0.6670622682304845 2306 78-79 0.8439696373246832 2306 80-81 1.0598600319433444 2306 82-83 1.0924792373597185 2306 84-85 1.1940909590836029 2306 86-87 1.1606011844145883 2306 88-89 1.1168751260286243 2306 90-91 1.3162379895756935 2306 92-93 1.3324544091848978 2306 94-95 1.3696709424715117 2306 96-97 1.5515979823108061 2306 98-99 1.3997230600436126 2306 100-101 1.2036498249620813 2307 1 0.07626859420229692 2307 2 0.06825274611796317 2307 3 0.09628211660412234 2307 4 0.1487459796619035 2307 5 0.1758525888746334 2307 6 0.1400147498006561 2307 7 0.24395670690179116 2307 8 0.21262112339456252 2307 9 0.2096463864241116 2307 10-11 0.29758635834195246 2307 12-13 0.17661022567775575 2307 14-15 0.3951359120086835 2307 16-17 0.4236453309655772 2307 18-19 0.5522282706010984 2307 20-21 0.5813157506074305 2307 22-23 0.5500824360417838 2307 24-25 0.6280149154903896 2307 26-27 0.6173936040431229 2307 28-29 0.7505922661265387 2307 30-31 0.7443429800039212 2307 32-33 0.6451426897993215 2307 34-35 0.6848798629283479 2307 36-37 0.5635841963531689 2307 38-39 0.6597359148146253 2307 40-41 0.5839961210253364 2307 42-43 0.6414536358625114 2307 44-45 0.7545670503910387 2307 46-47 0.6547790613793083 2307 48-49 0.7201945530684171 2307 50-51 0.5754519162702039 2307 52-53 0.7039779127021859 2307 54-55 0.6000018555089568 2307 56-57 0.5215543696414855 2307 58-59 0.5094348613288773 2307 60-61 0.4921677622472842 2307 62-63 0.5610746057527578 2307 64-65 0.41152470982491707 2307 66-67 0.651343083926399 2307 68-69 0.6107018484434192 2307 70-71 0.5755322960798992 2307 72-73 0.582947003955951 2307 74-75 0.5228355363241235 2307 76-77 0.3847113613091899 2307 78-79 0.5931590037012455 2307 80-81 0.6558873521975528 2307 82-83 0.6953439769053986 2307 84-85 0.7532846705268454 2307 86-87 0.8467291705601028 2307 88-89 0.8928434263118916 2307 90-91 0.981305041807456 2307 92-93 0.9416099640221418 2307 94-95 1.0957278535207244 2307 96-97 1.0058588516598128 2307 98-99 1.1146699029082185 2307 100-101 0.7002314779232393 2201 1 0.03890477087090005 2201 2 0.0773145257276866 2201 3 0.08496462601495125 2201 4 0.09978809437083669 2201 5 0.15564551314434283 2201 6 0.0749466278774662 2201 7 0.14842405658214375 2201 8 0.16181297015099005 2201 9 0.1784885102363205 2201 10-11 0.2082155097687135 2201 12-13 0.20812720593516332 2201 14-15 0.3586092974524746 2201 16-17 0.4116619120864371 2201 18-19 0.5257440484219771 2201 20-21 0.45985759614826094 2201 22-23 0.5061599593808026 2201 24-25 0.545609534248733 2201 26-27 0.5712779328658328 2201 28-29 0.5721152585666189 2201 30-31 0.7289801075808313 2201 32-33 0.8044015516450642 2201 34-35 0.7559418878512147 2201 36-37 0.8356958763478417 2201 38-39 0.8187469043679769 2201 40-41 0.8991571493590058 2201 42-43 0.9288576287634243 2201 44-45 0.8112586060437579 2201 46-47 0.9018675216262437 2201 48-49 0.9134398868633724 2201 50-51 1.021030603932651 2201 52-53 0.9812045178809257 2201 54-55 0.9260234741406279 2201 56-57 0.8968547731285881 2201 58-59 0.8966782856297542 2201 60-61 0.8934448529431407 2201 62-63 0.9674748474830821 2201 64-65 0.9100255942866298 2201 66-67 0.8495853476511783 2201 68-69 1.0238831156594372 2201 70-71 0.9700224203980881 2201 72-73 0.8953644369824474 2201 74-75 0.9775394340428036 2201 76-77 0.754864667397289 2201 78-79 1.0224521527194739 2201 80-81 1.0178741253956858 2201 82-83 0.9490948275954239 2201 84-85 0.9709972479800726 2201 86-87 1.171446809104907 2201 88-89 1.115566186453961 2201 90-91 1.3025815329302084 2201 92-93 1.5061781370370042 2201 94-95 1.535306057503167 2201 96-97 1.5457646455649687 2201 98-99 1.516035284398484 2201 100-101 1.2347520817591544 1101 1 0.12566093867389583 1101 2 0.09417120942126189 1101 3 0.12607676574195636 1101 4 0.13664232545092858 1101 5 0.1527740078090858 1101 6 0.0965659370425982 1101 7 0.14191736875412175 1101 8 0.15187328364648778 1101 9 0.06746830390319758 1101 10-11 0.12872516813087742 1101 12-13 0.1589637765477434 1101 14-15 0.3572175300803906 1101 16-17 0.48625874517816925 1101 18-19 0.49308162425948154 1101 20-21 0.5222597742538753 1101 22-23 0.4601840197500806 1101 24-25 0.38795963310423787 1101 26-27 0.4921403813564069 1101 28-29 0.43368198013374837 1101 30-31 0.5202134338115982 1101 32-33 0.5501757860987411 1101 34-35 0.5468557636522533 1101 36-37 0.5488157739916701 1101 38-39 0.47853624038476994 1101 40-41 0.6249891275106094 1101 42-43 0.6583297408481883 1101 44-45 0.5957611092444566 1101 46-47 0.490235218231021 1101 48-49 0.7207526031691103 1101 50-51 0.5997924236398191 1101 52-53 0.5678350841458055 1101 54-55 0.6319464531648364 1101 56-57 0.7779625192774873 1101 58-59 0.6819073301849627 1101 60-61 0.6501824016797499 1101 62-63 0.7691468828865382 1101 64-65 0.5634028149870147 1101 66-67 0.7849854727245642 1101 68-69 0.7353016158582406 1101 70-71 0.762182078603459 1101 72-73 0.7908439039367821 1101 74-75 0.76642808819674 1101 76-77 0.6714576538800223 1101 78-79 0.9853401991666217 1101 80-81 0.9047174550545023 1101 82-83 0.8858927442933862 1101 84-85 0.8747483436925734 1101 86-87 0.9182983944653387 1101 88-89 1.09522831006403 1101 90-91 1.1129019191821747 1101 92-93 1.2451342380615742 1101 94-95 1.3448572374891832 1101 96-97 1.3421157805919073 1101 98-99 1.2786589404331394 1101 100-101 1.0869807168302401 2304 1 0.10349149702433635 2304 2 0.07399577464325091 2304 3 0.0910132266619641 2304 4 0.07971991384341948 2304 5 0.09272489015412333 2304 6 0.0633233327231224 2304 7 0.08034647741173728 2304 8 0.10227634052326806 2304 9 0.11767347616248713 2304 10-11 0.08519047722970896 2304 12-13 0.1748787553610427 2304 14-15 0.3004092740153226 2304 16-17 0.33398808561431537 2304 18-19 0.40104688915211995 2304 20-21 0.3889668544343081 2304 22-23 0.49249138921461366 2304 24-25 0.538132487561704 2304 26-27 0.4565730117988167 2304 28-29 0.47762676037344676 2304 30-31 0.5566427865414099 2304 32-33 0.4920161265509435 2304 34-35 0.5879068022125082 2304 36-37 0.642790027526253 2304 38-39 0.7508374374943898 2304 40-41 0.6677379389345077 2304 42-43 0.6415084976200305 2304 44-45 0.747346910606602 2304 46-47 0.7115446881275034 2304 48-49 0.8378762647685463 2304 50-51 0.640999497290629 2304 52-53 0.7786195781455518 2304 54-55 0.7679893882576891 2304 56-57 0.8006635609974708 2304 58-59 0.773045107443707 2304 60-61 0.8083439675725685 2304 62-63 0.7579772771712285 2304 64-65 0.6742539259883813 2304 66-67 0.6745220102285998 2304 68-69 0.8023748643887458 2304 70-71 0.6442605290541792 2304 72-73 0.8090617325257483 2304 74-75 0.7466740007003025 2304 76-77 0.6872969793160841 2304 78-79 0.8460926530604667 2304 80-81 0.7286490049595358 2304 82-83 0.7301303211157091 2304 84-85 0.8262141503924951 2304 86-87 0.7944863138518627 2304 88-89 0.879165642048612 2304 90-91 0.9884915164225099 2304 92-93 0.8779438919128495 2304 94-95 1.0843460338094282 2304 96-97 0.864284096447161 2304 98-99 1.176295117637963 2304 100-101 0.887358698515218 2202 1 0.09183125161035832 2202 2 0.04152389824012204 2202 3 0.05988537747798972 2202 4 0.0768989872190744 2202 5 0.08917309878377466 2202 6 0.056828442749008445 2202 7 0.09781192353246837 2202 8 0.045743221013474056 2202 9 0.08944822733189284 2202 10-11 0.09057918255211206 2202 12-13 0.09759163698763729 2202 14-15 0.057894441023194076 2202 16-17 0.15727295423541676 2202 18-19 0.31713993218929915 2202 20-21 0.3487843955945422 2202 22-23 0.30311831973843795 2202 24-25 0.36207677334660815 2202 26-27 0.3966400709418352 2202 28-29 0.2565902895995791 2202 30-31 0.34514561769418606 2202 32-33 0.3209110864150091 2202 34-35 0.3678787243285413 2202 36-37 0.4275299075349466 2202 38-39 0.26363698402234803 2202 40-41 0.17003367503761524 2202 42-43 0.3442134150421978 2202 44-45 0.3233474984407394 2202 46-47 0.31661895636543136 2202 48-49 0.3728708164671346 2202 50-51 0.305137483904538 2202 52-53 0.3172582274273168 2202 54-55 0.336774989301162 2202 56-57 0.41480347771193493 2202 58-59 0.42164375285172895 2202 60-61 0.3714814848672816 2202 62-63 0.4141678546234573 2202 64-65 0.4565015382508193 2202 66-67 0.4833693880346104 2202 68-69 0.4819611050065902 2202 70-71 0.3288099047512958 2202 72-73 0.409217088690248 2202 74-75 0.38139928643984433 2202 76-77 0.24282157049031028 2202 78-79 0.4685460208150616 2202 80-81 0.5646138215965948 2202 82-83 0.7618302927398446 2202 84-85 0.6400447092344805 2202 86-87 0.6646506839246058 2202 88-89 0.800401624270016 2202 90-91 0.8086481322315784 2202 92-93 0.7055154800199048 2202 94-95 0.7402639591612648 2202 96-97 0.9013043733387072 2202 98-99 0.773809757620036 2202 100-101 0.7417725676083471 1102 1 0.05248669111788473 1102 2 0.04150047544749924 1102 3 0.09145955110815862 1102 4 0.08589358876373154 1102 5 0.13230666975047 1102 6 0.10304908794727652 1102 7 -0.030966839469982688 1102 8 0.06042694478897914 1102 9 0.05118697099808145 1102 10-11 0.05038292945139489 1102 12-13 0.04768420685421404 1102 14-15 0.08943746732276026 1102 16-17 0.15017080220405177 1102 18-19 0.28843779162015437 1102 20-21 0.2581412405942132 1102 22-23 0.4054293270804905 1102 24-25 0.40134340321790063 1102 26-27 0.45895796807341327 1102 28-29 0.4413736972919722 1102 30-31 0.4163651727358868 1102 32-33 0.3446361950257497 1102 34-35 0.4198390127279126 1102 36-37 0.4001378236913098 1102 38-39 0.3577307026024741 1102 40-41 0.49228563949975523 1102 42-43 0.44859123465430883 1102 44-45 0.481053908685972 1102 46-47 0.302658224428086 1102 48-49 0.42600230679215656 1102 50-51 0.4192453558316984 1102 52-53 0.5672590372579123 1102 54-55 0.5147492167539198 1102 56-57 0.642901546951208 1102 58-59 0.5679395877060855 1102 60-61 0.4629838701298681 1102 62-63 0.5805957043238763 1102 64-65 0.5690869286576969 1102 66-67 0.6814443584134935 1102 68-69 0.6358332165588862 1102 70-71 0.7144040151648454 1102 72-73 0.6865823858295528 1102 74-75 0.4701040184668983 1102 76-77 0.4214107085439984 1102 78-79 0.686597892333566 1102 80-81 0.6959730163508837 1102 82-83 0.7449850065145611 1102 84-85 0.7900042799993514 1102 86-87 0.8138323189601238 1102 88-89 0.8644729292310345 1102 90-91 0.9142749598012081 1102 92-93 0.923019781466266 1102 94-95 1.1242927916222634 1102 96-97 1.0873786510009573 1102 98-99 0.8991362750868319 1102 100-101 0.9581839092235498 2305 1 -0.0012743893255802163 2305 2 -0.03365808745159171 2305 3 0.01456996516028397 2305 4 0.04594528645921514 2305 5 0.0498080483099983 2305 6 0.0771232601605405 2305 7 -0.03788681997141907 2305 8 -0.013626486213837552 2305 9 -0.07142145093844476 2305 10-11 0.05019573317421333 2305 12-13 -0.03636383782984609 2305 14-15 -0.059656201171478074 2305 16-17 0.04862338912183617 2305 18-19 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-0.4307471713113671 1213 72-73 -0.4730887183696666 1213 74-75 -0.515862024798416 1213 76-77 -2.844474355784829 1213 78-79 -2.519425415321493 1213 80-81 -1.9507792140646387 1213 82-83 -1.5926921037056552 1213 84-85 -1.327922981245088 1213 86-87 -1.2486010306821669 1213 88-89 -1.3215890955419312 1213 90-91 -2.493258278008266 1213 92-93 -1.830479799160699 1213 94-95 -1.515212772559284 1213 96-97 -1.5231378143832863 1213 98-99 -3.386438166123554 1213 100-101 -1.8245896567952506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 29.0 9 94.0 10 153.0 11 121.0 12 204.0 13 428.0 14 1361.0 15 3517.0 16 6654.0 17 10461.0 18 13176.0 19 14686.0 20 16708.0 21 19236.0 22 22048.0 23 25430.0 24 29700.0 25 33924.0 26 39837.0 27 46505.0 28 55149.0 29 66227.0 30 78204.0 31 94160.0 32 120193.0 33 161138.0 34 233965.0 35 384802.0 36 719114.0 37 913123.0 38 238358.0 39 55.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.896402213553 31.471155994820005 20.071758891560254 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33.995075492835184 16.91695571631482 20-21 19.449420577090702 29.9001302869149 33.583609828465015 17.066839307529378 22-23 19.354922996939468 29.535607625860656 33.641332635364165 17.468136741835714 24-25 19.320850714129367 28.978431737331068 34.004003866502345 17.696713682037217 26-27 19.375094624492686 29.398960153346177 33.7844899743577 17.441455247803443 28-29 19.185681399370456 29.777487985862244 33.63830166542093 17.39852894934637 30-31 18.524019167674748 30.85493061169154 33.451426690990075 17.169623529643633 32-33 18.303161495752313 31.234921671076755 33.465133244723496 16.996783588447435 34-35 18.652857786591646 30.671967529502687 33.93527699631178 16.739897687593896 36-37 18.8030233262322 30.300958981259342 34.3105900031116 16.58542768939686 38-39 19.122661218268778 30.11901150040373 33.61253684954873 17.145790431778764 40-41 18.93500029787132 29.962943016395766 33.1894791552041 17.91257753052882 42-43 19.13671713674222 29.63494675722075 33.12537309064935 18.102963015387687 44-45 18.750362076698746 29.498022837259885 33.370884793017545 18.38073029302382 46-47 18.93276309867845 29.8301241457903 33.21375467625964 18.023358079271613 48-49 18.81061452180301 30.492486457107702 32.72001600659376 17.976883014495524 50-51 19.359475390879545 30.371118306428542 31.8141935699665 18.455212732725414 52-53 19.259201192134658 30.296376263022175 31.84794310483588 18.596479440007286 54-55 19.649519318506634 30.35579719473283 31.82118355739848 18.17349992936206 56-57 19.611282620713176 30.430887138850505 31.665875734797606 18.291954505638714 58-59 19.33394124611567 31.04868240430003 31.617025102238067 18.00035124734623 60-61 19.22638082019342 30.93933178882263 31.456770250424267 18.377517140559682 62-63 18.7790219334069 30.807947929623165 31.837828071595276 18.57520206537466 64-65 18.75569595094139 30.438041866648707 32.14119372945519 18.66506845295471 66-67 18.994192710254744 30.381376525716696 31.770065857372053 18.8543649066565 68-69 19.368758477984947 30.469605313114517 31.702339050095276 18.45929715880526 70-71 18.9439795620704 30.70652843620832 31.890949115332734 18.45854288638855 72-73 18.92319052623215 30.58998066126871 31.68037563857542 18.80645317392372 74-75 19.216454637814373 30.20143811393249 31.271972969121865 19.310134279131276 76-77 19.2837177540506 29.92009776582623 30.907942457725568 19.8882420223976 78-79 18.921173091018133 30.125015456315225 31.188409943891337 19.765401508775305 80-81 18.681428122637126 30.538636762708606 30.757798007331854 20.022137107322415 82-83 18.248932516275413 30.74384481672785 30.568660726363735 20.438561940633004 84-85 18.209150229203686 30.901076585387706 31.039823207059776 19.84994997834884 86-87 18.441347607128137 30.629566349847366 31.329670296846345 19.599415746178156 88-89 18.798883628568582 30.53891491170124 31.197419925649918 19.46478153408026 90-91 18.652634308089205 30.54644116681567 30.775227673862958 20.025696851232166 92-93 18.29228037493734 30.688390839140073 30.683237810623066 20.336090975299516 94-95 18.071171313531895 30.545597548622638 30.70766554280008 20.675565595045388 96-97 18.13357207092765 31.017754913694358 30.40212139501976 20.446551620358235 98-99 18.65924699991934 30.761887548881074 30.06034516237927 20.518520288820312 100-101 18.769545371248363 30.809576203257077 29.626462836482077 20.79441558901249 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 150992.0 1 83989.5 2 13973.0 3 11188.0 4 10059.0 5 8115.0 6 7723.5 7 8001.0 8 8017.0 9 7658.5 10 6964.5 11 6174.5 12 5555.5 13 5137.0 14 4872.0 15 4754.5 16 4663.5 17 4615.0 18 4704.5 19 4838.5 20 4940.0 21 5268.0 22 5738.5 23 6306.5 24 7133.5 25 8377.5 26 10249.5 27 12880.5 28 15914.0 29 19212.5 30 23777.0 31 29535.5 32 38332.0 33 53544.0 34 74047.0 35 96403.0 36 125937.0 37 164408.5 38 193018.0 39 196881.0 40 196067.0 41 195686.0 42 187512.5 43 181994.5 44 171688.0 45 158614.0 46 153810.5 47 142947.0 48 119533.0 49 96118.5 50 77324.0 51 62324.0 52 50448.0 53 39774.0 54 31465.0 55 25970.5 56 20652.5 57 15354.5 58 11070.0 59 7861.0 60 5776.0 61 4475.0 62 3364.0 63 2550.0 64 2038.0 65 1544.0 66 1080.5 67 756.0 68 562.0 69 466.0 70 347.0 71 249.0 72 212.5 73 163.5 74 121.0 75 94.0 76 75.0 77 60.0 78 51.0 79 39.5 80 25.5 81 16.5 82 19.0 83 19.5 84 13.5 85 7.5 86 5.5 87 3.5 88 1.5 89 2.5 90 2.5 91 2.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.176244183299923 2 0.006987654844490339 3 0.001970877007420352 4 0.0 5 0.0014333650963057104 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.5382506913746957E-4 12-13 0.0 14-15 2.98617728397023E-5 16-17 0.0 18-19 4.479265925955345E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.98617728397023E-5 38-39 3.284795012367253E-4 40-41 4.479265925955345E-5 42-43 0.001373641550626306 44-45 7.465443209925575E-4 46-47 0.00597235456794046 48-49 0.004270233516077429 50-51 0.008630052350673965 52-53 0.00441954238027594 54-55 0.02102268807915042 56-57 0.011705814953163302 58-59 0.0207539321235931 60-61 0.010317242516117145 62-63 0.0065098664790551024 64-65 0.013183972708728566 66-67 0.03207154402984027 68-69 0.03350490912614598 70-71 0.03767062643728446 72-73 0.03216112934835938 74-75 0.03535633904220752 76-77 0.034997997768131096 78-79 0.018424713842096322 80-81 0.0025979742370541 82-83 0.0 84-85 0.00636055761485659 86-87 0.02696518087425118 88-89 0.02844333862981644 90-91 0.026338083644617434 92-93 0.0363417775459177 94-95 0.03266877948663432 96-97 0.03447541674343631 98-99 0.040402978652117214 100-101 0.028174582674259124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3348763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.132533250660465 #Duplication Level Percentage of deduplicated Percentage of total 1 80.9134376725604 34.090881031643946 2 9.121330154651737 7.686094920622325 3 2.9505984275820887 3.7294855907834648 4 1.709903455197372 2.8817025672608994 5 1.0875543956157512 2.2910710867591293 6 0.6806056832894821 1.7205384949069553 7 0.6355680844742263 1.8744665416518245 8 0.3862507636845946 1.3018978515227344 9 0.30940203578203723 1.173230240436785 >10 1.8956983991941285 15.009033598368054 >50 0.16589418696682148 4.851405058517429 >100 0.13108723765260505 11.695821340027903 >500 0.010382319240477727 2.897072439668147 >1k 0.0021543416081857545 1.2177700077162952 >5k 0.0 0.0 >10k+ 1.3284249995744238E-4 7.579529230114273 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 169812 5.070887369455527 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101031 3.016964771767963 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT 4276 0.12768894066256703 TruSeq Adapter, Index 19 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.9861772839702303E-5 0.0 0.0 2 5.972354567940461E-5 0.0 0.0 3 8.95853185191069E-5 2.9861772839702303E-5 0.0 4 1.493088641985115E-4 2.9861772839702303E-5 0.0 5 1.791706370382138E-4 2.9861772839702303E-5 0.0 6 2.98617728397023E-4 2.9861772839702303E-5 0.0 7 3.8820304691612994E-4 2.9861772839702303E-5 0.0 8 5.97235456794046E-4 2.9861772839702303E-5 0.0 9 8.659914123513668E-4 2.9861772839702303E-5 0.0 10-11 0.002299356508657077 2.9861772839702303E-5 0.0 12-13 0.010063417446979675 2.9861772839702303E-5 0.0 14-15 0.02336683724706705 2.9861772839702303E-5 0.0 16-17 0.057827323104083506 2.9861772839702303E-5 0.0 18-19 0.09797647668706325 2.9861772839702303E-5 0.0 20-21 0.1094881901167685 2.9861772839702303E-5 0.0 22-23 0.11799879537608365 2.9861772839702303E-5 0.0 24-25 0.12770387154898688 2.9861772839702303E-5 0.0 26-27 0.13757318747250852 2.9861772839702303E-5 0.0 28-29 0.14957762015406884 2.9861772839702303E-5 0.0 30-31 0.1622688736109423 2.9861772839702303E-5 0.0 32-33 0.17609487443572447 2.9861772839702303E-5 0.0 34-35 0.1906674195814992 2.9861772839702303E-5 0.0 36-37 0.20594171638900693 2.9861772839702303E-5 0.0 38-39 0.2239483654113474 2.9861772839702303E-5 0.0 40-41 0.24310469268801643 2.9861772839702303E-5 0.0 42-43 0.26332111290049487 2.9861772839702303E-5 0.0 44-45 0.28598619848582896 2.9861772839702303E-5 0.0 46-47 0.3097263078933923 2.9861772839702303E-5 0.0 48-49 0.33484005885158197 2.9861772839702303E-5 0.0 50-51 0.3618649632715125 2.9861772839702303E-5 0.0 52-53 0.3896662737852753 2.9861772839702303E-5 0.0 54-55 0.41952804662497767 2.9861772839702303E-5 0.0 56-57 0.4505992212646879 2.9861772839702303E-5 0.0 58-59 0.48468643496120806 2.9861772839702303E-5 0.0 60-61 0.5201472902083546 4.479265925955346E-5 0.0 62-63 0.5576984695542802 5.972354567940461E-5 0.0 64-65 0.5981611717520767 5.972354567940461E-5 0.0 66-67 0.6423565955548363 5.972354567940461E-5 0.0 68-69 0.6903743262810775 5.972354567940461E-5 0.0 70-71 0.7435432128221675 5.972354567940461E-5 0.0 72-73 0.7991458338496932 5.972354567940461E-5 0.0 74-75 0.8629903041809768 5.972354567940461E-5 0.0 76-77 0.9370027081641787 5.972354567940461E-5 0.0 78-79 1.0203767779326278 5.972354567940461E-5 0.0 80-81 1.1171587837061028 5.972354567940461E-5 0.0 82-83 1.2278115829636196 5.972354567940461E-5 0.0 84-85 1.355366145648408 5.972354567940461E-5 0.0 86-87 1.5011961133110945 5.972354567940461E-5 0.0 88-89 1.6655851727936555 5.972354567940461E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGACG 30 1.2917483E-4 63.430542 1 CCGCGTG 25 0.0049917176 57.08749 1 CGAGTCG 45 1.5028927E-5 52.858784 1 CGCGGAG 160 0.0 50.546215 1 GTCGCGC 40 5.349067E-4 47.572906 1 CGTGCCG 40 5.349067E-4 47.572906 1 ACGACTG 65 2.563671E-6 43.91345 1 CCGGGCG 100 6.2755134E-10 42.815617 1 CGCAGCC 200 0.0 40.43697 1 CGACGGG 120 8.54925E-11 39.64409 1 CGGACAG 100 2.992965E-8 38.058327 1 CGTGCGG 50 0.0016041255 38.058327 1 CGCACGA 50 0.0016041255 38.058327 1 CGGACGG 100 1.2846904E-6 33.301033 1 CGCGAGG 75 3.0521175E-4 31.715273 1 ACGATTG 75 3.0521175E-4 31.715273 1 CGGGGTG 540 0.0 31.715271 1 CGGGTGG 425 0.0 31.342152 1 GTTTGAT 13715 0.0 31.231407 3 AGGGCGC 260 0.0 31.10536 1 >>END_MODULE