FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_50220371-2.34100000035905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_50220371-2.34100000035905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348763
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1698125.070887369455527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010313.016964771767963No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT42760.12768894066256703TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG301.2917483E-463.4305421
CCGCGTG250.004991717657.087491
CGAGTCG451.5028927E-552.8587841
CGCGGAG1600.050.5462151
GTCGCGC405.349067E-447.5729061
CGTGCCG405.349067E-447.5729061
ACGACTG652.563671E-643.913451
CCGGGCG1006.2755134E-1042.8156171
CGCAGCC2000.040.436971
CGACGGG1208.54925E-1139.644091
CGGACAG1002.992965E-838.0583271
CGTGCGG500.001604125538.0583271
CGCACGA500.001604125538.0583271
CGGACGG1001.2846904E-633.3010331
CGCGAGG753.0521175E-431.7152731
ACGATTG753.0521175E-431.7152731
CGGGGTG5400.031.7152711
CGGGTGG4250.031.3421521
GTTTGAT137150.031.2314073
AGGGCGC2600.031.105361
Produced by FastQC (version 0.11.2)