##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_50110001-2.341000000358f7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5962269 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.695496462839905 33.0 33.0 33.0 33.0 33.0 2 32.71717998634413 33.0 33.0 33.0 33.0 33.0 3 32.74405566068891 33.0 33.0 33.0 33.0 33.0 4 36.68156820834484 37.0 37.0 37.0 37.0 37.0 5 36.636285950868704 37.0 37.0 37.0 37.0 37.0 6 36.51461817640231 37.0 37.0 37.0 37.0 37.0 7 36.46210025076024 37.0 37.0 37.0 37.0 37.0 8 36.38378174483573 37.0 37.0 37.0 37.0 37.0 9 36.382326426399075 37.0 37.0 37.0 37.0 37.0 10-11 36.321719633917894 37.0 37.0 37.0 37.0 37.0 12-13 36.334616318049385 37.0 37.0 37.0 37.0 37.0 14-15 38.175548939506086 40.0 37.0 40.0 37.0 40.0 16-17 38.16212728744711 40.0 37.0 40.0 37.0 40.0 18-19 38.15120384538169 40.0 37.0 40.0 37.0 40.0 20-21 38.059395005492036 40.0 37.0 40.0 37.0 40.0 22-23 37.96080904769644 40.0 37.0 40.0 33.0 40.0 24-25 37.93431284297975 40.0 37.0 40.0 33.0 40.0 26-27 37.84431841300686 40.0 37.0 40.0 33.0 40.0 28-29 37.7317089349709 40.0 37.0 40.0 33.0 40.0 30-31 37.667576471306475 40.0 37.0 40.0 33.0 40.0 32-33 37.54142416251263 40.0 37.0 40.0 33.0 40.0 34-35 37.430461792314304 40.0 37.0 40.0 33.0 40.0 36-37 37.339628923149895 40.0 37.0 40.0 33.0 40.0 38-39 37.27422664089795 40.0 37.0 40.0 33.0 40.0 40-41 37.14678212271201 40.0 37.0 40.0 33.0 40.0 42-43 37.01983422753988 40.0 37.0 40.0 33.0 40.0 44-45 36.907085456895686 37.0 37.0 40.0 33.0 40.0 46-47 36.78131873956039 37.0 37.0 40.0 33.0 40.0 48-49 36.568243063169405 37.0 37.0 40.0 33.0 40.0 50-51 36.39955636017093 37.0 37.0 40.0 33.0 40.0 52-53 36.26883649496526 37.0 37.0 40.0 33.0 40.0 54-55 36.092251205036206 37.0 37.0 40.0 33.0 40.0 56-57 35.90394470628548 37.0 37.0 40.0 30.0 40.0 58-59 35.67177780807944 37.0 37.0 40.0 27.0 40.0 60-61 35.39966202464196 37.0 33.0 40.0 27.0 40.0 62-63 35.10938495059515 37.0 33.0 38.5 27.0 40.0 64-65 34.7918138212147 37.0 33.0 37.0 27.0 40.0 66-67 34.47779486299595 37.0 33.0 37.0 27.0 40.0 68-69 34.23157299679032 37.0 33.0 37.0 27.0 40.0 70-71 33.97827454615013 37.0 33.0 37.0 27.0 40.0 72-73 33.65234217040526 37.0 33.0 37.0 27.0 37.0 74-75 33.28601200314847 37.0 33.0 37.0 27.0 37.0 76-77 30.504143472895972 33.0 30.0 35.0 24.5 37.0 78-79 33.0079946912828 35.0 33.0 37.0 27.0 37.0 80-81 33.44361441927562 37.0 33.0 37.0 27.0 37.0 82-83 33.396864431980504 37.0 33.0 37.0 27.0 37.0 84-85 33.30359339707752 37.0 33.0 37.0 27.0 37.0 86-87 33.15932726282561 37.0 33.0 37.0 27.0 37.0 88-89 33.02514026119922 37.0 33.0 37.0 27.0 37.0 90-91 32.886086488214474 37.0 33.0 37.0 27.0 37.0 92-93 32.73253663999394 33.0 33.0 37.0 27.0 37.0 94-95 32.66537395411042 33.0 33.0 37.0 27.0 37.0 96-97 32.54580143901592 33.0 33.0 37.0 27.0 37.0 98-99 32.22357260298051 33.0 33.0 37.0 22.0 37.0 100-101 30.555521564022023 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09608986102107053 2306 2 0.10939844049013203 2306 3 0.14597314495154023 2306 4 0.1753676747590518 2306 5 0.1659431191920433 2306 6 0.22190399130131055 2306 7 0.24948695518541797 2306 8 0.28856480670128093 2306 9 0.2390861831118869 2306 10-11 0.27828985350621593 2306 12-13 0.2595100364324594 2306 14-15 0.4867058129472781 2306 16-17 0.5718659822490011 2306 18-19 0.5225510040613486 2306 20-21 0.5232471085384276 2306 22-23 0.5174796135039585 2306 24-25 0.556188327589318 2306 26-27 0.4594854835581401 2306 28-29 0.54999483083445 2306 30-31 0.5842060036983625 2306 32-33 0.5278466136516542 2306 34-35 0.5219892309333645 2306 36-37 0.5141132496388963 2306 38-39 0.5718406998072894 2306 40-41 0.6177433066383955 2306 42-43 0.6200644247287244 2306 44-45 0.5783172194429298 2306 46-47 0.6461499367884258 2306 48-49 0.6576935610898644 2306 50-51 0.6487197369410254 2306 52-53 0.5147854496768502 2306 54-55 0.5069766748158955 2306 56-57 0.5527363705662509 2306 58-59 0.5487353932098742 2306 60-61 0.4746271007402356 2306 62-63 0.6630291045182659 2306 64-65 0.5840926091375209 2306 66-67 0.5158363858262973 2306 68-69 0.5563849040513063 2306 70-71 0.4682967356594929 2306 72-73 0.5411878818397398 2306 74-75 0.5621891728640307 2306 76-77 0.1112225369575306 2306 78-79 0.45706366988500946 2306 80-81 0.42952475888905894 2306 82-83 0.6493678423140636 2306 84-85 0.5537151299073244 2306 86-87 0.5229824953808659 2306 88-89 0.4649421857644427 2306 90-91 0.7233119622979416 2306 92-93 0.5879104050846493 2306 94-95 0.6124604512138632 2306 96-97 0.6059593869561795 2306 98-99 0.5458714796383006 2306 100-101 0.3487101935114687 2307 1 0.13902711511094878 2307 2 0.1058824606461286 2307 3 0.12815938088720458 2307 4 0.16081669802129284 2307 5 0.1900864200825012 2307 6 0.18497764354124513 2307 7 0.19965855731145865 2307 8 0.19376422687108175 2307 9 0.13894509109835695 2307 10-11 0.2465876377265488 2307 12-13 0.22948504851224527 2307 14-15 0.4460478437334956 2307 16-17 0.43243214501561766 2307 18-19 0.40993707433123916 2307 20-21 0.41143334447409785 2307 22-23 0.4576093850232539 2307 24-25 0.4219442466892005 2307 26-27 0.5090377718165726 2307 28-29 0.5195188178572963 2307 30-31 0.515833669881836 2307 32-33 0.5257505275054584 2307 34-35 0.5012919160993761 2307 36-37 0.5043740351707555 2307 38-39 0.5590517165809459 2307 40-41 0.4950083028418888 2307 42-43 0.5195875834227337 2307 44-45 0.5045902918526792 2307 46-47 0.4790414947369399 2307 48-49 0.5008627472567824 2307 50-51 0.33457933520153205 2307 52-53 0.43523302336360814 2307 54-55 0.38440595624062013 2307 56-57 0.3567635673633873 2307 58-59 0.3545011142011063 2307 60-61 0.41903974656062104 2307 62-63 0.4285772511324737 2307 64-65 0.28082098630324737 2307 66-67 0.38082623879792976 2307 68-69 0.44909105439300134 2307 70-71 0.3000717063228464 2307 72-73 0.19827285457390786 2307 74-75 0.09727152255685212 2307 76-77 -0.2437727001515988 2307 78-79 0.14346955101981962 2307 80-81 0.2634017520553442 2307 82-83 0.24984385529121766 2307 84-85 0.2863907701364923 2307 86-87 0.37775132260527755 2307 88-89 0.3236773276157692 2307 90-91 0.3757740317395104 2307 92-93 0.4570635768938587 2307 94-95 0.5326292922437474 2307 96-97 0.5570150920711043 2307 98-99 0.4495754901304636 2307 100-101 0.09242745726517754 2201 1 0.07320086269545811 2201 2 0.0616965461403467 2201 3 0.07530188194224507 2201 4 0.12821576563531778 2201 5 0.15635147140321237 2201 6 0.13551034738653556 2201 7 0.18449309458592467 2201 8 0.2594754734992648 2201 9 0.17924153949259392 2201 10-11 0.2288778570519625 2201 12-13 0.21897016316018636 2201 14-15 0.38995660909948526 2201 16-17 0.4285751322539255 2201 18-19 0.4064764119867519 2201 20-21 0.37141441540995856 2201 22-23 0.3644680241543412 2201 24-25 0.40729939492574374 2201 26-27 0.4470276878327297 2201 28-29 0.3472274386022747 2201 30-31 0.4871470063576737 2201 32-33 0.5238572509017345 2201 34-35 0.45660809563673865 2201 36-37 0.49932143112877014 2201 38-39 0.4714806091936765 2201 40-41 0.5608837905676154 2201 42-43 0.5328502679934388 2201 44-45 0.46325027699570853 2201 46-47 0.5603493523962939 2201 48-49 0.6231272260164857 2201 50-51 0.5827699683997096 2201 52-53 0.5791528944386641 2201 54-55 0.5650670914415272 2201 56-57 0.5667435277283417 2201 58-59 0.4302743800446436 2201 60-61 0.5510869976508346 2201 62-63 0.5706646802101787 2201 64-65 0.457612028685098 2201 66-67 0.3593754383230916 2201 68-69 0.4735420004760158 2201 70-71 0.5305761276290255 2201 72-73 0.4551174264735067 2201 74-75 0.4315288097529617 2201 76-77 0.15612512732490913 2201 78-79 0.48346256676277477 2201 80-81 0.545130392691874 2201 82-83 0.5424494273956526 2201 84-85 0.4330021715042278 2201 86-87 0.4946280517024775 2201 88-89 0.5869971448898355 2201 90-91 0.6307047197752098 2201 92-93 0.6059446150061873 2201 94-95 0.7837744142461318 2201 96-97 0.7268927071570985 2201 98-99 0.5978534882400268 2201 100-101 0.45558907988331043 1101 1 0.10224993350672662 1101 2 0.10574028082034204 1101 3 0.10271335435454176 1101 4 0.1335814571007461 1101 5 0.17136957645571016 1101 6 0.10977719174427847 1101 7 0.112772115761274 1101 8 0.21268048172392895 1101 9 0.1863218118242571 1101 10-11 0.10746042131050615 1101 12-13 0.14575243550625316 1101 14-15 0.35399837310030335 1101 16-17 0.36468265394939436 1101 18-19 0.3461071301058425 1101 20-21 0.3682547598018928 1101 22-23 0.3685470150946131 1101 24-25 0.31410174750273967 1101 26-27 0.3800508716584119 1101 28-29 0.30650051894479446 1101 30-31 0.33084825679409846 1101 32-33 0.36837311621886215 1101 34-35 0.3735852492561236 1101 36-37 0.38092685076645694 1101 38-39 0.4192857315197571 1101 40-41 0.4474188326627626 1101 42-43 0.4403141478737993 1101 44-45 0.523389151386958 1101 46-47 0.4690968058108851 1101 48-49 0.514402561391897 1101 50-51 0.4805379942650987 1101 52-53 0.4478341006382891 1101 54-55 0.4645379100922469 1101 56-57 0.49837117374757156 1101 58-59 0.4869831028645635 1101 60-61 0.38073278381683906 1101 62-63 0.43311948506794806 1101 64-65 0.40817002784284284 1101 66-67 0.4570226588555997 1101 68-69 0.5157312495662651 1101 70-71 0.44539552253154113 1101 72-73 0.46594160679695307 1101 74-75 0.3431652878343314 1101 76-77 0.15962996673705376 1101 78-79 0.5351061041948881 1101 80-81 0.46008289842394845 1101 82-83 0.4886511907485769 1101 84-85 0.344803975170592 1101 86-87 0.4275750404327141 1101 88-89 0.5432796970903269 1101 90-91 0.42128269118146733 1101 92-93 0.5641378036550719 1101 94-95 0.5406385192698338 1101 96-97 0.555436735189339 1101 98-99 0.5200531216397124 1101 100-101 0.4000429227976845 2304 1 0.08234278774458659 2304 2 0.08063484266770615 2304 3 0.11812600904558224 2304 4 0.12448122681378493 2304 5 0.1441325974403469 2304 6 0.11881602097804489 2304 7 0.12012076546628947 2304 8 0.1682928020763299 2304 9 0.17314534387692504 2304 10-11 0.10581695279802261 2304 12-13 0.12636076148906028 2304 14-15 0.3113068150188667 2304 16-17 0.291206060294968 2304 18-19 0.3238406653804162 2304 20-21 0.33327596627317035 2304 22-23 0.38530058956023083 2304 24-25 0.37989495667830653 2304 26-27 0.39455637530549836 2304 28-29 0.38893935557309334 2304 30-31 0.39870665408136574 2304 32-33 0.43377167507672 2304 34-35 0.41293688714572596 2304 36-37 0.420402116045409 2304 38-39 0.47943565572577285 2304 40-41 0.4644645573008219 2304 42-43 0.4770554157197182 2304 44-45 0.4815581983897985 2304 46-47 0.4167189014750434 2304 48-49 0.513650563546868 2304 50-51 0.47223845351856397 2304 52-53 0.5690623923949687 2304 54-55 0.566181906785836 2304 56-57 0.6381978775277588 2304 58-59 0.5790080963943396 2304 60-61 0.539181997883361 2304 62-63 0.533446000023396 2304 64-65 0.46047679278640175 2304 66-67 0.43664625719499384 2304 68-69 0.4910478611260274 2304 70-71 0.5046556266360369 2304 72-73 0.48659497342330127 2304 74-75 0.502867281453895 2304 76-77 0.35851557063292105 2304 78-79 0.523586144231011 2304 80-81 0.4635144250257852 2304 82-83 0.5221959725833685 2304 84-85 0.5029975165720657 2304 86-87 0.5084829771460591 2304 88-89 0.5885098255967876 2304 90-91 0.5766354435331849 2304 92-93 0.609625585505718 2304 94-95 0.5689062767185078 2304 96-97 0.5546391817242053 2304 98-99 0.6234807198945944 2304 100-101 0.48462287357120815 2202 1 0.10231048081676164 2202 2 0.09098724639621736 2202 3 0.08204182037432162 2202 4 0.12339754856796503 2202 5 0.14231259296661847 2202 6 0.1362911489972518 2202 7 0.15255777671516313 2202 8 0.155235593893309 2202 9 0.16144106335911346 2202 10-11 0.12167211807827982 2202 12-13 0.14523894020101835 2202 14-15 0.20764117220558376 2202 16-17 0.19088426708032102 2202 18-19 0.2048348052459943 2202 20-21 0.18377659604808372 2202 22-23 0.3251558088855404 2202 24-25 0.3337496633262944 2202 26-27 0.3225748474520316 2202 28-29 0.17870012742643127 2202 30-31 0.25315441128699945 2202 32-33 0.2596148340997715 2202 34-35 0.223776165434181 2202 36-37 0.25076154562997033 2202 38-39 0.3573374806468408 2202 40-41 0.28801337273849015 2202 42-43 0.3145346796862327 2202 44-45 0.28711076395318713 2202 46-47 0.2524594912566229 2202 48-49 0.26662857697105835 2202 50-51 0.232975552890899 2202 52-53 0.29658199873685476 2202 54-55 0.356117030211216 2202 56-57 0.32644947898758403 2202 58-59 0.2764507335824433 2202 60-61 0.35380230891260567 2202 62-63 0.36631625362977616 2202 64-65 0.348115958781257 2202 66-67 0.37371397188518785 2202 68-69 0.36732730254045265 2202 70-71 0.25640283918769313 2202 72-73 0.2500538789210296 2202 74-75 0.2381764679108116 2202 76-77 0.12941019255028863 2202 78-79 0.282681662845782 2202 80-81 0.4218118221562932 2202 82-83 0.4282923851932807 2202 84-85 0.3966067085836045 2202 86-87 0.36471757993748355 2202 88-89 0.353378274699125 2202 90-91 0.3947203235306702 2202 92-93 0.3866179336278748 2202 94-95 0.4349492485355171 2202 96-97 0.4345445015582854 2202 98-99 0.4797731020951588 2202 100-101 0.3024971280122877 1102 1 0.06154107754822036 1102 2 0.059461788020357176 1102 3 0.0490983550492885 1102 4 0.07274206486365387 1102 5 0.11260573892736403 1102 6 0.1108259083718508 1102 7 0.10101953461460766 1102 8 0.14660093535452745 1102 9 0.09675704357434256 1102 10-11 0.08605800350376569 1102 12-13 0.05707639468379 1102 14-15 0.13773702980078895 1102 16-17 0.15781164240468115 1102 18-19 0.16744185114281862 1102 20-21 0.17914130086557378 1102 22-23 0.08914750404100147 1102 24-25 0.16643013173742816 1102 26-27 0.15370281029888844 1102 28-29 0.1843100672046134 1102 30-31 0.2379032344876748 1102 32-33 0.19197565854322107 1102 34-35 0.16095732871244905 1102 36-37 0.2504361555967023 1102 38-39 0.2605841602599597 1102 40-41 0.21180254196418957 1102 42-43 0.15313496861671894 1102 44-45 0.2922413394801353 1102 46-47 0.20587644255693505 1102 48-49 0.17777965629066728 1102 50-51 0.18751029192544166 1102 52-53 0.27674612264236487 1102 54-55 0.23020710512872 1102 56-57 0.31651300606502275 1102 58-59 0.22387718267153645 1102 60-61 0.09716145886101657 1102 62-63 0.23128432228559603 1102 64-65 0.2507030104038179 1102 66-67 0.1974000678810981 1102 68-69 0.37650193869500015 1102 70-71 0.34392396106377987 1102 72-73 0.17213417840800815 1102 74-75 0.07003245218388088 1102 76-77 0.022984616558058946 1102 78-79 0.29030816654792346 1102 80-81 0.29972903101256065 1102 82-83 0.35043584055168964 1102 84-85 0.22356607881481239 1102 86-87 0.29440541602511416 1102 88-89 0.307412902626119 1102 90-91 0.4777282414056643 1102 92-93 0.3977868104957878 1102 94-95 0.4421608151113148 1102 96-97 0.40840503895271496 1102 98-99 0.3097049679952093 1102 100-101 0.2496944463794044 2305 1 0.06921419073799484 2305 2 0.012646102293345507 2305 3 0.04973408771247989 2305 4 0.07761546126609886 2305 5 0.05193800633507806 2305 6 0.0737777910269628 2305 7 0.04614264791518252 2305 8 0.09476420812290343 2305 9 0.060600336996408544 2305 10-11 0.025620879818724518 2305 12-13 0.04910799128967369 2305 14-15 0.04008447612223165 2305 16-17 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27 61831.0 28 78098.0 29 98942.0 30 116918.0 31 142991.0 32 189484.0 33 264425.0 34 399933.0 35 681025.0 36 1287279.0 37 1927358.0 38 464590.0 39 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.136945858824944 28.976699768378744 16.38917814944259 16.497176223353726 2 33.45142837014927 23.456217140106325 28.353661144625047 14.738693345119355 3 39.211814794389674 20.572504692936374 21.885168308355773 18.330512204318172 4 33.32939188084268 20.533541844556158 27.203670280559294 18.933395994041867 5 35.176653959373674 18.556192327219197 26.893325995988388 19.37382771741874 6 32.21037158840032 19.507422425925434 28.63654759622553 19.645658389448712 7 35.95235303875085 20.037975475443996 22.177597153030163 21.83207433277499 8 31.07905731861478 25.979891212556833 22.176607596872934 20.76444387195546 9 29.481846592295653 22.542072489516997 26.8392620326255 21.136818885561855 10-11 22.61265541946231 30.126116776010136 31.409256099876355 15.851971704651197 12-13 22.48715212279084 29.31055274426565 31.54506279404703 16.65723233889648 14-15 22.40366417611553 29.01630721732066 31.80951443309828 16.770514173465536 16-17 22.168808552582917 28.8489080247805 31.832059237850558 17.15022418478603 18-19 21.043190625845945 29.213037459247875 31.888999920500027 17.854771994406153 20-21 21.630900920438176 29.034969740546764 31.21446717684157 18.119662162173494 22-23 20.966103676301756 28.551672190570404 31.518688606636164 18.963535526491675 24-25 20.532091054596833 27.75767916543182 32.194379354571225 19.515850425400128 26-27 20.531529187965187 28.195499062521336 31.632311457265683 19.640660292247798 28-29 20.723092165080107 28.752359210897733 30.93791138910371 19.586637234918452 30-31 19.74029517957006 30.42740943087271 30.507949238788118 19.324346150769113 32-33 19.407317918731945 31.142824988272082 31.017369394101475 18.432487698894498 34-35 20.0298074441123 30.28517331237487 32.170931905286395 17.514087338226435 36-37 19.672270686255633 29.905708700112104 33.20194597089564 17.220074642736623 38-39 20.153158584470983 29.40864705220838 31.785039594522303 18.65315476879833 40-41 20.415863735018174 29.088489937256234 31.364805877899936 19.13084044982566 42-43 20.394236704882836 28.789757168498532 31.419533746006806 19.396472380611822 44-45 20.02020052763899 28.30827038349219 31.81980606051051 19.85172302835831 46-47 20.742422643900703 28.636076740779554 30.97941593182489 19.642084683494858 48-49 20.486863676023788 29.780747055778768 30.420262065629455 19.31212720256799 50-51 21.455065096090074 29.573189448425158 28.91390891545878 20.057836540025985 52-53 21.04874822669222 29.518483829814784 28.787825673907275 20.64494226958572 54-55 21.72207520814847 29.36453768908415 29.118650604560138 19.794736498207243 56-57 21.245234396367216 30.0183734217011 29.015412891219395 19.720979290712286 58-59 20.803241504571588 30.994165633992214 28.765199270800707 19.43739359063549 60-61 21.016310698600176 30.993220810069204 28.481018125617013 19.509450365713608 62-63 20.404312636816222 30.739580413762614 29.238000637713917 19.61810631170725 64-65 20.82327609018503 29.666881629361875 29.87031199113579 19.63953028931731 66-67 21.046830125659476 29.64861047776372 29.312558867752703 19.992000528824104 68-69 21.24444408652159 30.367567759830184 29.407744488715025 18.9802436649332 70-71 20.53481761605879 30.97076106989362 29.528835368089585 18.965585945958008 72-73 20.316742214301385 30.400659019766817 29.58744574055207 19.695153025379728 74-75 20.584723755079963 29.43327066186905 29.306470842668897 20.67553474038209 76-77 20.609286898866962 28.739624847331392 28.87695214273784 21.774136111063807 78-79 19.917247055058027 29.294795812547665 29.287314145149047 21.500642987245268 80-81 19.45275524385445 29.906097191570385 28.919703421030295 21.721444143544865 82-83 18.620100837449634 30.117259050203877 28.972158082770168 22.290482029576324 84-85 18.34295542911718 30.54506833450592 29.957594791110964 21.154381445265933 86-87 18.796317950469593 30.201241397405877 30.60027996702402 20.40216068510051 88-89 19.587280704182607 30.00063080760318 30.050281741156486 20.361806747057727 90-91 19.53764602223618 30.171584515737653 29.45485255512071 20.835916906905457 92-93 18.917714068653375 30.609319170811983 29.65315938675307 20.819807373781572 94-95 19.064955023652114 30.036692445332534 29.79301568446846 21.105336846546894 96-97 19.380859759382535 30.714186605291154 29.34374889371233 20.561204741613984 98-99 20.176259144298836 30.419291657976586 28.699285100776677 20.705164096947897 100-101 19.868713211640074 30.970814253227225 27.93799800859475 21.222474526537948 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11143.0 1 6348.0 2 1625.5 3 1717.0 4 1416.0 5 962.0 6 825.0 7 850.5 8 870.0 9 871.0 10 829.5 11 755.0 12 726.5 13 715.5 14 713.5 15 710.5 16 768.0 17 857.5 18 946.5 19 1113.5 20 1418.5 21 1841.5 22 2406.5 23 3402.0 24 5004.5 25 7092.5 26 9552.0 27 13067.0 28 17943.5 29 23611.0 30 31025.5 31 40128.0 32 57043.0 33 91912.0 34 140195.5 35 184756.5 36 241692.0 37 326647.0 38 406429.5 39 449689.5 40 453016.0 41 441888.0 42 443408.0 43 437323.0 44 388583.0 45 321777.0 46 276963.5 47 241702.5 48 204370.0 49 164368.0 50 120211.5 51 90060.5 52 73536.5 53 56483.0 54 40649.5 55 29611.5 56 22175.0 57 16903.0 58 12910.0 59 9697.5 60 7132.5 61 5578.5 62 4406.5 63 3430.5 64 2599.5 65 1962.5 66 1546.0 67 1215.0 68 966.0 69 815.5 70 708.5 71 572.0 72 428.0 73 300.0 74 225.5 75 182.0 76 138.0 77 113.0 78 86.0 79 52.0 80 38.0 81 28.0 82 16.5 83 11.5 84 9.0 85 7.0 86 5.5 87 3.5 88 2.0 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17293751757929743 2 0.006775943856273509 3 0.0019623401761980214 4 0.0 5 0.0012746825076158088 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.8512635038774667E-4 12-13 0.0 14-15 1.6772138258102745E-5 16-17 0.0 18-19 5.031641477430824E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.6772138258102745E-5 38-39 2.9351241951679804E-4 40-41 5.870248390335961E-5 42-43 0.0015011063741001958 44-45 8.134487055179832E-4 46-47 0.0055012613486577 48-49 0.0042098067027837895 50-51 0.007832588566533983 52-53 0.004142718149751378 54-55 0.020118179840594243 56-57 0.011212174425541685 58-59 0.02029428729230432 60-61 0.00953496059973141 62-63 0.0058367041138197556 64-65 0.012906160389610063 66-67 0.03144775923394265 68-69 0.03275598601807466 70-71 0.03720898872560094 72-73 0.031892220897782375 74-75 0.03500345254466043 76-77 0.0344751301895302 78-79 0.017292074544103933 80-81 0.0024655043239411034 82-83 0.0 84-85 0.006432115021982403 86-87 0.026281940650447002 88-89 0.027774660955418147 90-91 0.02571168794967151 92-93 0.03564079379846834 94-95 0.03210187262600865 96-97 0.03387971928136754 98-99 0.040387308925511414 100-101 0.02774111667890194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5962269.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.08552076522972 #Duplication Level Percentage of deduplicated Percentage of total 1 80.44085452933214 29.027501264910384 2 9.206391169753712 6.644348394579502 3 3.1819346070871064 3.444653020129345 4 1.7458750636082703 2.5200324344533196 5 1.1436381592647658 2.0634389272028897 6 0.6509848414041329 1.4094676207403167 7 0.47617057254593237 1.2028004158378296 8 0.40180856518891317 1.1599577058217347 9 0.27650944651106096 0.898018863647136 >10 2.012109587782296 14.386608798516221 >50 0.2315457432230974 5.840864596564077 >100 0.19132165554783978 14.460192392029256 >500 0.025295992062683563 6.372522007395625 >1k 0.015280183131464696 9.1232707307048 >5k 1.399437175026342E-4 0.3601440888840715 >10k+ 1.39939839132794E-4 1.0861787385832196 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 30732 0.5154413529480136 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19021 0.31902284180737234 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14775 0.24780834276346805 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7686 0.1289106546517777 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7507 0.1259084419035773 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6202 0.10402080147675324 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.708855303241098E-4 0.0 0.0 2 7.715183598727264E-4 0.0 0.0 3 9.056954659375483E-4 0.0 0.0 4 9.727840189699593E-4 0.0 0.0 5 0.0011740496780671922 0.0 0.0 6 0.0015430367197454527 0.0 0.0 7 0.001844935208391302 0.0 0.0 8 0.0022642386648438705 0.0 0.0 9 0.00256613715348972 0.0 0.0 10-11 0.003622781863750193 0.0 0.0 12-13 0.005668982731238729 0.0 0.0 14-15 0.009065340728504533 0.0 0.0 16-17 0.016470239769456895 0.0 1.6772138258102745E-5 18-19 0.02637418741086657 0.0 1.6772138258102745E-5 20-21 0.03361975113836695 0.0 1.6772138258102745E-5 22-23 0.04206452275132169 0.0 1.6772138258102745E-5 24-25 0.05124726844763294 0.0 1.6772138258102745E-5 26-27 0.06140279816291415 0.0 1.6772138258102745E-5 28-29 0.07263174472671394 0.0 1.6772138258102745E-5 30-31 0.08530309518071057 0.0 1.6772138258102745E-5 32-33 0.09853631226635363 0.0 1.6772138258102745E-5 34-35 0.11287649047703148 0.0 1.6772138258102745E-5 36-37 0.12863391437051902 0.0 1.6772138258102745E-5 38-39 0.14473516709829765 0.0 1.6772138258102745E-5 40-41 0.1625220197210156 0.0 1.6772138258102745E-5 42-43 0.18155839664396223 0.0 1.6772138258102745E-5 44-45 0.20169334862281457 0.0 1.6772138258102745E-5 46-47 0.22218051550508705 0.0 1.6772138258102745E-5 48-49 0.2445964782870414 0.0 1.6772138258102745E-5 50-51 0.2681697186088048 0.0 1.6772138258102745E-5 52-53 0.2930260275073131 0.0 1.6772138258102745E-5 54-55 0.3184609751757259 0.0 1.6772138258102745E-5 56-57 0.3466884838641128 0.0 1.6772138258102745E-5 58-59 0.3772221615629888 0.0 1.6772138258102745E-5 60-61 0.40892150287080303 0.0 1.6772138258102745E-5 62-63 0.44512416330091786 0.0 1.6772138258102745E-5 64-65 0.4835994484650055 0.0 1.6772138258102745E-5 66-67 0.5268128626870072 0.0 1.6772138258102745E-5 68-69 0.5749572855568912 0.0 1.6772138258102745E-5 70-71 0.6282759130793998 0.0 1.6772138258102745E-5 72-73 0.6893097242006356 0.0 1.6772138258102745E-5 74-75 0.7632161514349655 0.0 1.6772138258102745E-5 76-77 0.8527793697332341 0.0 1.6772138258102745E-5 78-79 0.9613538067470622 0.0 1.6772138258102745E-5 80-81 1.096168254065692 0.0 1.6772138258102745E-5 82-83 1.2653236544677873 0.0 1.6772138258102745E-5 84-85 1.4761075020264935 0.0 1.6772138258102745E-5 86-87 1.723605560232187 0.0 1.6772138258102745E-5 88-89 2.0111890288747456 0.0 1.6772138258102745E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAG 340 0.0 55.96959 1 CGACGGG 340 0.0 54.57035 1 CGAGTGG 300 0.0 52.331566 1 CGGAGCG 85 1.2732926E-10 50.37263 1 CGCGGGG 440 0.0 47.574154 1 CGGACGG 115 1.8189894E-12 45.505707 1 CGACGTG 65 2.563549E-6 43.9146 1 CGAGTCG 55 4.9203223E-5 43.249233 1 CGAGGGG 1065 0.0 41.09692 1 CCAGCCG 375 0.0 40.527493 2 CACGGGG 540 0.0 39.64513 1 TCAGCCG 340 0.0 39.11201 2 CGGGTGG 610 0.0 38.995205 1 CGGGCTG 840 0.0 38.51241 1 CGGGACG 50 0.0016040201 38.059322 1 ATCGGCC 125 1.3278623E-10 38.05932 1 ACGTGCG 65 1.329979E-4 36.5332 2 GTGTCGA 225 0.0 35.88161 5 GTTACGG 295 0.0 35.479027 1 GGACGGG 710 0.0 34.84304 1 >>END_MODULE