FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_50110001-2.341000000358f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_50110001-2.341000000358f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5962269
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT307320.5154413529480136TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC190210.31902284180737234No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147750.24780834276346805No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG76860.1289106546517777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75070.1259084419035773No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA62020.10402080147675324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG3400.055.969591
CGACGGG3400.054.570351
CGAGTGG3000.052.3315661
CGGAGCG851.2732926E-1050.372631
CGCGGGG4400.047.5741541
CGGACGG1151.8189894E-1245.5057071
CGACGTG652.563549E-643.91461
CGAGTCG554.9203223E-543.2492331
CGAGGGG10650.041.096921
CCAGCCG3750.040.5274932
CACGGGG5400.039.645131
TCAGCCG3400.039.112012
CGGGTGG6100.038.9952051
CGGGCTG8400.038.512411
CGGGACG500.001604020138.0593221
ATCGGCC1251.3278623E-1038.059321
ACGTGCG651.329979E-436.53322
GTGTCGA2250.035.881615
GTTACGG2950.035.4790271
GGACGGG7100.034.843041
Produced by FastQC (version 0.11.2)