FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_44065-1.341000000357de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_44065-1.341000000357de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868037
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT203030.5248915664457191TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC200.00207631771.233833
CGACTTA250.00502694356.9863245
CGACGGG1300.051.2409321
CGCTCGG601.4758116E-647.5808681
AGCGCGG502.7999558E-547.5808641
TGGGCGC804.2346073E-947.493522
CGGCAGC1052.0008883E-1145.315111
CGTGCGC554.9159054E-543.2553331
AACCGAG2200.041.0925641
CGAGGTG2550.039.1842421
AGTCGCC853.5953963E-739.1842421
GTGACGC1101.5952537E-938.92981
CGAGCGT756.846143E-638.0646931
GCGCGTT500.00161819537.9908834
GTATGCG905.627153E-737.007341
TCGCCGG651.3298336E-436.5334742
TTGTGTT239900.036.4740331
CGAGGGG4400.035.685651
GTCCGGC958.716306E-734.9952242
ACGTGCG550.002583250634.5376133
Produced by FastQC (version 0.11.2)