##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_44065-1.341000000357de.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3868037 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.690397222156875 33.0 33.0 33.0 33.0 33.0 2 32.72101921465591 33.0 33.0 33.0 33.0 33.0 3 32.74196446414551 33.0 33.0 33.0 33.0 33.0 4 36.688680072088246 37.0 37.0 37.0 37.0 37.0 5 36.650101071939076 37.0 37.0 37.0 37.0 37.0 6 36.53243156670942 37.0 37.0 37.0 37.0 37.0 7 36.49312429017613 37.0 37.0 37.0 37.0 37.0 8 36.43535131644294 37.0 37.0 37.0 37.0 37.0 9 36.44568937680793 37.0 37.0 37.0 37.0 37.0 10-11 36.40228971439518 37.0 37.0 37.0 37.0 37.0 12-13 36.393393082847965 37.0 37.0 37.0 37.0 37.0 14-15 38.28315848581593 40.0 37.0 40.0 37.0 40.0 16-17 38.25724999528184 40.0 37.0 40.0 37.0 40.0 18-19 38.24924283299255 40.0 37.0 40.0 37.0 40.0 20-21 38.16578486710442 40.0 37.0 40.0 37.0 40.0 22-23 38.08845378159516 40.0 37.0 40.0 37.0 40.0 24-25 38.05945354193871 40.0 37.0 40.0 35.0 40.0 26-27 37.975386481566744 40.0 37.0 40.0 35.0 40.0 28-29 37.86745615411642 40.0 37.0 40.0 33.0 40.0 30-31 37.808869718671254 40.0 37.0 40.0 33.0 40.0 32-33 37.699468490089416 40.0 37.0 40.0 33.0 40.0 34-35 37.622955390550814 40.0 37.0 40.0 33.0 40.0 36-37 37.55229086484954 40.0 37.0 40.0 33.0 40.0 38-39 37.46557607902923 40.0 37.0 40.0 33.0 40.0 40-41 37.379753477022064 40.0 37.0 40.0 33.0 40.0 42-43 37.27283748836942 40.0 37.0 40.0 33.0 40.0 44-45 37.16874541272485 40.0 37.0 40.0 33.0 40.0 46-47 37.056800258115416 40.0 37.0 40.0 33.0 40.0 48-49 36.858602696923526 37.0 37.0 40.0 33.0 40.0 50-51 36.69879036834446 37.0 37.0 40.0 33.0 40.0 52-53 36.56877778056415 37.0 37.0 40.0 33.0 40.0 54-55 36.40413974840468 37.0 37.0 40.0 33.0 40.0 56-57 36.21478168383601 37.0 37.0 40.0 33.0 40.0 58-59 35.98958761252801 37.0 37.0 40.0 33.0 40.0 60-61 35.71660883802301 37.0 37.0 40.0 27.0 40.0 62-63 35.4658758434834 37.0 33.0 40.0 27.0 40.0 64-65 35.167362799270016 37.0 33.0 37.0 27.0 40.0 66-67 34.8556996740207 37.0 33.0 37.0 27.0 40.0 68-69 34.582538248729264 37.0 33.0 37.0 27.0 40.0 70-71 34.29118723528239 37.0 33.0 37.0 27.0 40.0 72-73 33.959311402657214 37.0 33.0 37.0 27.0 37.0 74-75 33.55860492027351 37.0 33.0 37.0 27.0 37.0 76-77 30.837897491673424 33.0 30.0 35.0 24.5 37.0 78-79 33.300068613614606 35.0 33.0 37.0 27.0 37.0 80-81 33.72222098702779 37.0 33.0 37.0 27.0 37.0 82-83 33.67106519405063 37.0 33.0 37.0 27.0 37.0 84-85 33.55122572509001 37.0 33.0 37.0 27.0 37.0 86-87 33.39656575156856 37.0 33.0 37.0 27.0 37.0 88-89 33.25515875365205 37.0 33.0 37.0 27.0 37.0 90-91 33.11357492185313 37.0 33.0 37.0 27.0 37.0 92-93 32.96359846092476 33.0 33.0 37.0 27.0 37.0 94-95 32.88055310742891 33.0 33.0 37.0 27.0 37.0 96-97 32.7489477220616 33.0 33.0 37.0 27.0 37.0 98-99 32.44981743969874 33.0 33.0 37.0 27.0 37.0 100-101 30.784733832690847 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14126444912997016 2306 2 0.14244032824512942 2306 3 0.15248108052238507 2306 4 0.19390512318535968 2306 5 0.19096558247983353 2306 6 0.23366738117924513 2306 7 0.27157483324761955 2306 8 0.34061488994065314 2306 9 0.2663657388096823 2306 10-11 0.28621856867239615 2306 12-13 0.2463556722212914 2306 14-15 0.527863882272456 2306 16-17 0.531296964033217 2306 18-19 0.5683809368285324 2306 20-21 0.6226055535404171 2306 22-23 0.5270692704029045 2306 24-25 0.5098947934878382 2306 26-27 0.5348779445923597 2306 28-29 0.4757028981784188 2306 30-31 0.553115147005947 2306 32-33 0.5748904742339462 2306 34-35 0.6666362267025718 2306 36-37 0.553091911544449 2306 38-39 0.5929952149376234 2306 40-41 0.7451337231647628 2306 42-43 0.7576118550244857 2306 44-45 0.6951205415719457 2306 46-47 0.6975505912510371 2306 48-49 0.6929670907874623 2306 50-51 0.647595578082381 2306 52-53 0.703771497009221 2306 54-55 0.7280407015522385 2306 56-57 0.5606892651061912 2306 58-59 0.6951966298001508 2306 60-61 0.6190541323698113 2306 62-63 0.6354210979325146 2306 64-65 0.59186461243835 2306 66-67 0.5771783559082522 2306 68-69 0.6374091565479816 2306 70-71 0.6105528098431705 2306 72-73 0.6297516901149507 2306 74-75 0.5908325990911649 2306 76-77 0.05877360862819003 2306 78-79 0.5257762301809237 2306 80-81 0.7199383101239434 2306 82-83 0.7376315069859629 2306 84-85 0.7738522309436959 2306 86-87 0.618675766928078 2306 88-89 0.7461489821312668 2306 90-91 0.7393881564695093 2306 92-93 0.6999363009578801 2306 94-95 0.8074218650378171 2306 96-97 0.8218002897285999 2306 98-99 0.6758185878299159 2306 100-101 0.42208995787588677 2307 1 0.10398303247981744 2307 2 0.11060802848910356 2307 3 0.12503698050146284 2307 4 0.13650566462487035 2307 5 0.19510753950207516 2307 6 0.17383702731730466 2307 7 0.17079224274237959 2307 8 0.2712004556026173 2307 9 0.2499495579519504 2307 10-11 0.2529090580230431 2307 12-13 0.27909294768738135 2307 14-15 0.5312788895818272 2307 16-17 0.5094032630984842 2307 18-19 0.4099687176933813 2307 20-21 0.48470644434521404 2307 22-23 0.46668753898659077 2307 24-25 0.5465734860089668 2307 26-27 0.5564576155203227 2307 28-29 0.5660688207389484 2307 30-31 0.5544894193660923 2307 32-33 0.5638538439433987 2307 34-35 0.5257526361967209 2307 36-37 0.5648781282098128 2307 38-39 0.6375547171519074 2307 40-41 0.6714188751708718 2307 42-43 0.6084825076658262 2307 44-45 0.5726200676743503 2307 46-47 0.588854616343987 2307 48-49 0.6343147635361817 2307 50-51 0.6127406398240325 2307 52-53 0.718730588450903 2307 54-55 0.5459645209030768 2307 56-57 0.5994875834905358 2307 58-59 0.5416680404322705 2307 60-61 0.5565442480008187 2307 62-63 0.566017460484737 2307 64-65 0.5829624537350995 2307 66-67 0.7100688736007399 2307 68-69 0.7000290342363868 2307 70-71 0.46316133239302815 2307 72-73 0.42829846549484785 2307 74-75 0.4906585605818492 2307 76-77 0.052881267120096 2307 78-79 0.4134593095521808 2307 80-81 0.5099512479884538 2307 82-83 0.5735699377193129 2307 84-85 0.547800070921511 2307 86-87 0.6289595595075426 2307 88-89 0.7666900658323144 2307 90-91 0.76524578842929 2307 92-93 0.6705952609351513 2307 94-95 0.715677621283298 2307 96-97 0.6904386975799071 2307 98-99 0.6476242266285368 2307 100-101 0.47311550908473876 2201 1 0.10381690307246316 2201 2 0.08408117906154189 2201 3 0.10286243223881542 2201 4 0.13153335471396588 2201 5 0.19611577698367455 2201 6 0.19716936290983256 2201 7 0.18897779360101197 2201 8 0.22128062722887165 2201 9 0.20478249942166116 2201 10-11 0.22099774642413905 2201 12-13 0.23108872601979868 2201 14-15 0.3527891983955911 2201 16-17 0.3709193803215314 2201 18-19 0.33793698731039257 2201 20-21 0.3754700721307742 2201 22-23 0.41524567370829857 2201 24-25 0.39970229360485376 2201 26-27 0.38333029897203375 2201 28-29 0.38578035866552085 2201 30-31 0.4798643232547235 2201 32-33 0.5495432921111316 2201 34-35 0.4645766781151863 2201 36-37 0.4528830954012051 2201 38-39 0.5627612105529138 2201 40-41 0.4395935213530606 2201 42-43 0.4998007717748649 2201 44-45 0.5127769837428389 2201 46-47 0.527091419181879 2201 48-49 0.48775930602380413 2201 50-51 0.5231665119477853 2201 52-53 0.5393404269170432 2201 54-55 0.5621233041192255 2201 56-57 0.5781435709924452 2201 58-59 0.5582074302377364 2201 60-61 0.49403975538540124 2201 62-63 0.5529350756542541 2201 64-65 0.5339798155377977 2201 66-67 0.5167419235831048 2201 68-69 0.6270989476777444 2201 70-71 0.5607285324615745 2201 72-73 0.5761648765321539 2201 74-75 0.5166131370408849 2201 76-77 0.23231721716777898 2201 78-79 0.5281557534036665 2201 80-81 0.6051845134828326 2201 82-83 0.48376747927113684 2201 84-85 0.4171316698920222 2201 86-87 0.5912326615653072 2201 88-89 0.6100505087847239 2201 90-91 0.6492959744207809 2201 92-93 0.7273506523518378 2201 94-95 0.7420890257944919 2201 96-97 0.7215127970817363 2201 98-99 0.7275513878408333 2201 100-101 0.4773448544592078 1101 1 0.054967541809872955 1101 2 0.07034602090884334 1101 3 0.11805521802507002 1101 4 0.13221264929267562 1101 5 0.17101499166324174 1101 6 0.12814734254517646 1101 7 0.116632212104399 1101 8 0.12226604993987422 1101 9 0.11584604911051599 1101 10-11 0.18774735021960254 1101 12-13 0.15302741938026543 1101 14-15 0.3438668747382536 1101 16-17 0.3331268345364933 1101 18-19 0.30606897785211373 1101 20-21 0.30208002964954517 1101 22-23 0.36132747665413945 1101 24-25 0.31859281963283337 1101 26-27 0.42787898023117776 1101 28-29 0.4311544618255496 1101 30-31 0.37780360395993995 1101 32-33 0.4151302064752542 1101 34-35 0.4517465878362472 1101 36-37 0.4043694320408804 1101 38-39 0.5331510353115192 1101 40-41 0.5173853582737564 1101 42-43 0.5431398772893274 1101 44-45 0.5887356468246736 1101 46-47 0.5085030718991064 1101 48-49 0.5511973487480688 1101 50-51 0.5000005212339076 1101 52-53 0.5693870600645496 1101 54-55 0.47811168887054123 1101 56-57 0.6395826668865965 1101 58-59 0.6035752548849445 1101 60-61 0.5725797986261227 1101 62-63 0.6017120314869331 1101 64-65 0.5591057655426042 1101 66-67 0.5786778827525154 1101 68-69 0.6757442066410775 1101 70-71 0.6347092108447541 1101 72-73 0.6290612456277032 1101 74-75 0.4897653048999544 1101 76-77 0.22831198169822997 1101 78-79 0.5766617711556492 1101 80-81 0.5520906243998454 1101 82-83 0.4919553688300695 1101 84-85 0.5162455894561688 1101 86-87 0.5607382793152667 1101 88-89 0.6689770856281498 1101 90-91 0.5332782224321662 1101 92-93 0.5359328730213164 1101 94-95 0.6403868905938097 1101 96-97 0.64306075428658 1101 98-99 0.63689168829805 1101 100-101 0.636809763142292 2304 1 0.09845598401388855 2304 2 0.07698730739868864 2304 3 0.09712727575912794 2304 4 0.10770857934995348 2304 5 0.14337527799651184 2304 6 0.160150576998646 2304 7 0.16818659602748198 2304 8 0.18157050222117022 2304 9 0.1749952289288359 2304 10-11 0.16493075822113923 2304 12-13 0.19674035696045422 2304 14-15 0.36637787632042773 2304 16-17 0.32310722683082815 2304 18-19 0.3780409428059741 2304 20-21 0.36437064903336136 2304 22-23 0.3789084606851958 2304 24-25 0.3669431782505299 2304 26-27 0.40061108849189253 2304 28-29 0.39554420412093094 2304 30-31 0.3314169605701238 2304 32-33 0.437447468033632 2304 34-35 0.41309500905369845 2304 36-37 0.35762012789241737 2304 38-39 0.3518331824838228 2304 40-41 0.4423901608762719 2304 42-43 0.5006099457410116 2304 44-45 0.3594637961517222 2304 46-47 0.4222613245463762 2304 48-49 0.4742776515340452 2304 50-51 0.37088604635351174 2304 52-53 0.45862979130810544 2304 54-55 0.43906038398824876 2304 56-57 0.5398004316081497 2304 58-59 0.5853225608703241 2304 60-61 0.45599326881610125 2304 62-63 0.6087387881761117 2304 64-65 0.4592660859965534 2304 66-67 0.3639541550916263 2304 68-69 0.3161531873909311 2304 70-71 0.41097916618405606 2304 72-73 0.42523140966402906 2304 74-75 0.566113691061922 2304 76-77 0.31119826401647543 2304 78-79 0.48138449389240634 2304 80-81 0.4042830650333755 2304 82-83 0.47867938628303364 2304 84-85 0.4802686045557394 2304 86-87 0.5769035823232613 2304 88-89 0.7104650744477894 2304 90-91 0.658948611992173 2304 92-93 0.5612002646355734 2304 94-95 0.546227229183124 2304 96-97 0.49113702569088247 2304 98-99 0.5303496322232775 2304 100-101 0.5852662230516614 2202 1 0.13725353884105118 2202 2 0.12427936048295152 2202 3 0.13123823775930532 2202 4 0.12602518523286932 2202 5 0.11642253676400571 2202 6 0.10490855700059853 2202 7 0.14215440921491762 2202 8 0.11269456851287174 2202 9 0.11873392461590981 2202 10-11 0.1436840241523143 2202 12-13 0.16240450056833566 2202 14-15 0.19211722801087205 2202 16-17 0.2532975198185099 2202 18-19 0.2015219023253323 2202 20-21 0.22400339289403348 2202 22-23 0.2916737506867122 2202 24-25 0.291541319840114 2202 26-27 0.28638792460355944 2202 28-29 0.20661367165387645 2202 30-31 0.20086934164220338 2202 32-33 0.271749666113017 2202 34-35 0.23518677220666717 2202 36-37 0.2719483667400553 2202 38-39 0.3310417414555644 2202 40-41 0.31552007884291555 2202 42-43 0.34199772573648346 2202 44-45 0.278432166699325 2202 46-47 0.33910236490809353 2202 48-49 0.32940544303731656 2202 50-51 0.363823131573092 2202 52-53 0.30934533600501624 2202 54-55 0.40483933685499096 2202 56-57 0.3929068173606751 2202 58-59 0.4121587765560548 2202 60-61 0.39073012114062067 2202 62-63 0.3653610590399765 2202 64-65 0.4381777618338063 2202 66-67 0.4015556392819306 2202 68-69 0.3798559433159028 2202 70-71 0.3376823175486905 2202 72-73 0.28912886891649947 2202 74-75 0.21886418111412098 2202 76-77 0.13485106902900768 2202 78-79 0.2675486303057468 2202 80-81 0.3891698065012932 2202 82-83 0.44667785479946787 2202 84-85 0.44161992470186107 2202 86-87 0.49240327434493025 2202 88-89 0.5492439234704491 2202 90-91 0.5238141269660872 2202 92-93 0.3027120074253915 2202 94-95 0.42660716331581483 2202 96-97 0.37776884188927795 2202 98-99 0.4778635111847649 2202 100-101 0.3548467709566374 1102 1 0.10169287179102326 1102 2 0.07199068883257809 1102 3 0.045027771974368136 1102 4 0.09761805469807427 1102 5 0.13158067362427062 1102 6 0.1569384592432712 1102 7 0.16782433445931133 1102 8 0.15732366569515932 1102 9 0.12347227999256205 1102 10-11 0.1125483139740524 1102 12-13 0.09138379901571625 1102 14-15 0.18159681397982297 1102 16-17 0.20123599020379146 1102 18-19 0.17158228185379443 1102 20-21 0.2459182865110634 1102 22-23 0.1981889893296085 1102 24-25 0.186345805176515 1102 26-27 0.19740862615617516 1102 28-29 0.3224848619931535 1102 30-31 0.22265485055537226 1102 32-33 0.2540124608226293 1102 34-35 0.2681597451796378 1102 36-37 0.24592191475615977 1102 38-39 0.25264611178333496 1102 40-41 0.2770899465829899 1102 42-43 0.17426914609301036 1102 44-45 0.28859317877522983 1102 46-47 0.25658080079079326 1102 48-49 0.2432956097998158 1102 50-51 0.3161549314580867 1102 52-53 0.26119409966228346 1102 54-55 0.1199543454806431 1102 56-57 0.2284387322542898 1102 58-59 0.3213910939798552 1102 60-61 0.22764788997328367 1102 62-63 0.33434035748735624 1102 64-65 0.3093278480902697 1102 66-67 0.27296449494610187 1102 68-69 0.3965120675252152 1102 70-71 0.36545402542677863 1102 72-73 0.34213059404588364 1102 74-75 0.18915457789388057 1102 76-77 0.10185796954654691 1102 78-79 0.2697625463494546 1102 80-81 0.24013587895672828 1102 82-83 0.33739953392539945 1102 84-85 0.3431552898542449 1102 86-87 0.40748665559852526 1102 88-89 0.3290944122570991 1102 90-91 0.4092597853904749 1102 92-93 0.33371833289979946 1102 94-95 0.49843005003231866 1102 96-97 0.443628531365988 1102 98-99 0.37499979640615777 1102 100-101 0.2747806409968909 2305 1 0.017830331906779406 2305 2 0.0157337680006151 2305 3 0.034181772267608324 2305 4 0.032331481635736736 2305 5 0.10657854413751267 2305 6 0.055999809407694556 2305 7 0.016337394147775797 2305 8 0.035159339136619394 2305 9 -0.00809732629675608 2305 10-11 0.005359969597641623 2305 12-13 0.042546743153344835 2305 14-15 -0.009060778513216405 2305 16-17 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-0.7040246607782024 1213 66-67 -0.7126734616370243 1213 68-69 -0.07201726206281478 1213 70-71 0.02468375266185774 1213 72-73 -0.010995051444538717 1213 74-75 0.08217312810368327 1213 76-77 -2.160077054826175 1213 78-79 -1.9154968223047888 1213 80-81 -1.2939429220151837 1213 82-83 -0.8942279501213193 1213 84-85 -0.5812529544616964 1213 86-87 -0.36383949979814645 1213 88-89 -0.3933397664296123 1213 90-91 -1.6755604751506645 1213 92-93 -0.7319601367827389 1213 94-95 -0.35936448069926286 1213 96-97 -0.3672986797661295 1213 98-99 -2.3065087178308055 1213 100-101 -0.8105982961828566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 9.0 8 62.0 9 221.0 10 303.0 11 229.0 12 165.0 13 231.0 14 423.0 15 804.0 16 1593.0 17 2640.0 18 3872.0 19 5035.0 20 6447.0 21 8648.0 22 10684.0 23 13720.0 24 17212.0 25 21660.0 26 27281.0 27 35099.0 28 45415.0 29 58831.0 30 71256.0 31 88919.0 32 119547.0 33 170303.0 34 260863.0 35 447812.0 36 848293.0 37 1272456.0 38 327249.0 39 755.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.93168363961648 32.23120954345778 15.809265516121496 19.027841300804248 2 30.72269454202388 24.706621793484597 29.930795087322497 14.639888577169025 3 32.61217743630552 22.950539522543814 24.30135598715291 20.135927053997758 4 28.446806480910087 22.799704346158013 28.60678426809258 20.14670490483933 5 31.440412203754402 21.10540616702732 27.860051344496757 19.594130284721523 6 28.508905163006453 21.505921479034456 30.169385659961375 19.81578769799772 7 31.157742286332834 22.3820506370544 24.608089322827055 21.852117753785706 8 27.595832201191456 25.27362070218046 25.304540778694722 21.82600631793336 9 26.74565936158315 22.621448553878878 28.341921238085366 22.290970846452606 10-11 22.03482134316435 29.264173235471286 31.024785226588214 17.676220194776153 12-13 22.089667187775092 28.61629813778927 30.105360936309555 19.188673738126084 14-15 22.089460364520814 28.44459605738001 30.388178810078603 19.077764768020575 16-17 22.38744096811897 27.80287520517513 30.62468120134321 19.185002625362685 18-19 21.57447603900274 27.841946145278897 31.012120879950444 19.57145693576792 20-21 22.00365456690306 28.048555378348244 30.591576554205663 19.356213500543042 22-23 21.568407954732592 28.94491443592706 29.936024396871076 19.55065321246927 24-25 21.32810518617066 28.125765084460152 30.542546516488855 20.003583212880333 26-27 21.295970023037526 28.292658524207496 30.432025339985113 19.979346112769864 28-29 21.362489552194045 29.164806334582632 30.02014975554784 19.452554357675485 30-31 20.725473929023945 29.42535192915683 30.26956567375131 19.57960846806791 32-33 20.551819954152457 29.791299824691443 29.953488035404003 19.7033921857521 34-35 21.052590758568236 29.434568490425505 30.232169444087532 19.280671306918727 36-37 20.608365589151703 28.94228492185698 31.16416186405711 19.285187624934206 38-39 20.96906523261927 29.03374627862555 30.407987122115117 19.58920136664007 40-41 20.803498521018877 29.10360546163691 30.35977316127868 19.73312285606553 42-43 21.089549350572632 29.126474007328625 30.02588298893131 19.75809365316743 44-45 20.63803326334474 28.79279185183395 30.408311867738757 20.16086301708255 46-47 21.028656378767067 29.004327381433303 30.007219843257655 19.959796396541975 48-49 20.681236144705093 29.7378758211377 29.96134446242587 19.61954357173134 50-51 21.184377529758557 29.323093168026254 29.363440029516163 20.12908927269902 52-53 20.728533271835406 29.074640610711654 29.298031661263625 20.898794456189318 54-55 21.290343433925525 29.096402754918525 29.570950012476516 20.042303798679427 56-57 20.98150367675719 28.823912153420984 29.67344273519036 20.52114143463147 58-59 21.009566216864417 29.316996163945745 29.61488187357699 20.058555745612843 60-61 21.076747160918046 29.4631324952313 29.325581891411296 20.134538452439358 62-63 20.596933811710485 29.45017308293425 29.24430664571763 20.70858645963764 64-65 20.90118548255105 29.26073919358021 29.299303769141048 20.538771554727695 66-67 21.025248338537182 29.492712217714246 29.045461698331344 20.43657774541723 68-69 21.07688185821724 29.65688968380339 29.120180159165077 20.146048298814293 70-71 20.972479299280284 30.236524973924478 28.91202029248552 19.878975434309726 72-73 20.669983066293856 30.15015604456761 28.92060036556944 20.259260523569093 74-75 20.858742360481813 29.606236370934035 28.856004133718983 20.67901713486517 76-77 20.94544312870331 29.233099367126176 28.93169454834729 20.889762955823226 78-79 20.61217975279937 28.846615552351235 29.687864631661338 20.85334006318806 80-81 20.596994319719812 29.24178303558248 29.328457011905297 20.832765632792416 82-83 20.12655256400081 29.552328480828905 29.224823857682853 21.096295097487435 84-85 20.170994332938015 29.536344924710217 29.313943961265725 20.97871678108604 86-87 20.29163919624798 29.343346335962263 29.79831982536507 20.566694642424686 88-89 20.445650209116728 29.81173417285173 29.502422950835783 20.24019266719576 90-91 20.720026593808665 29.565687470253604 28.946432378763692 20.76785355717404 92-93 20.385722772118474 29.67647514449701 29.003276446592942 20.93452563679158 94-95 19.984635926278308 29.824651463621333 28.935883374758493 21.25482923534187 96-97 20.047132543954817 30.22967578757899 28.836595543291466 20.886596125174727 98-99 20.29473603627218 29.941010636241224 28.716894374292085 21.04735895319451 100-101 20.406034308092718 30.003244126534124 28.31875502086443 21.27196654450873 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 208.0 1 124.5 2 77.0 3 137.5 4 132.5 5 75.0 6 52.0 7 65.0 8 71.0 9 69.5 10 81.0 11 84.5 12 81.5 13 97.0 14 111.0 15 119.5 16 142.5 17 167.5 18 208.5 19 286.5 20 443.5 21 740.0 22 1146.0 23 1724.5 24 2663.0 25 4252.5 26 7081.5 27 11163.0 28 15869.0 29 20990.5 30 26574.0 31 31356.5 32 38683.0 33 54257.5 34 76884.5 35 109163.0 36 152929.5 37 204883.0 38 256596.5 39 275990.5 40 281488.0 41 270574.0 42 238021.5 43 223151.5 44 210184.0 45 200861.0 46 205430.0 47 191745.5 48 157117.0 49 127991.5 50 103039.5 51 83769.5 52 69231.0 53 55247.0 54 44710.0 55 34431.5 56 23813.5 57 15444.0 58 10116.0 59 7182.5 60 5312.5 61 3946.5 62 2851.5 63 1949.0 64 1325.5 65 919.0 66 676.0 67 511.0 68 369.5 69 254.5 70 188.5 71 129.0 72 90.5 73 69.5 74 49.5 75 38.5 76 32.5 77 28.5 78 22.0 79 15.0 80 10.0 81 5.5 82 3.0 83 5.5 84 4.0 85 0.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17567050160068273 2 0.006411520882556191 3 0.0020682325427600614 4 0.0 5 0.001215086618871536 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.8097034749150538E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.5852906784500768E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.5852906784500768E-5 38-39 2.7145552123725804E-4 40-41 6.463226696125192E-5 42-43 0.0013572776061862902 44-45 6.592491230047697E-4 46-47 0.005803977573120423 48-49 0.00404597991177437 50-51 0.00842804761174725 52-53 0.0040330534583821204 54-55 0.019958444037634592 56-57 0.01112967637072758 58-59 0.0202169731054796 60-61 0.009552649056873034 62-63 0.006152991814711183 64-65 0.012874747578681383 66-67 0.03072617971337916 68-69 0.03190248697207395 70-71 0.03578042298974906 72-73 0.030842517793909414 74-75 0.0336346317266355 76-77 0.03388023434108826 78-79 0.017903137948266784 80-81 0.002430173237743072 82-83 0.0 84-85 0.006411520882556191 86-87 0.02581412742432402 88-89 0.027352375378001812 90-91 0.025116098941142497 92-93 0.03484971834550704 94-95 0.03164395790422894 96-97 0.033143426497729986 98-99 0.0385854633758674 100-101 0.027106772763549055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3868037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.90831769014931 #Duplication Level Percentage of deduplicated Percentage of total 1 87.61726107443118 36.71892012248015 2 5.578368071332786 4.675600426519997 3 1.8097464408613366 2.2753028634670174 4 0.9818235343941656 1.6458629038022372 5 0.5989187219195103 1.2549838034390515 6 0.48231985641958863 1.212792826265959 7 0.30950424824495953 0.9079561653330419 8 0.23847473407815964 0.7995259933457112 9 0.2616569211683699 0.986904124033536 >10 1.6867303868348278 14.567133354548002 >50 0.23020072696013655 6.830468575607819 >100 0.16920014226178937 14.153851744976082 >500 0.02570640312479935 7.51866732303072 >1k 0.010026209880516846 5.9200004242554245 >5k 0.0 0.0 >10k+ 6.252808791352831E-5 0.5320293488952442 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 20303 0.5248915664457191 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.824104578110293E-4 0.0 0.0 2 0.001215086618871536 0.0 0.0 3 0.0016287331274235483 0.0 2.5852906784500768E-5 4 0.0019648209156220586 0.0 5.1705813569001536E-5 5 0.0022750557970360676 0.0 5.1705813569001536E-5 6 0.0034125836955541016 0.0 5.1705813569001536E-5 7 0.0042140238058736255 0.0 5.1705813569001536E-5 8 0.005222287170469155 0.0 5.1705813569001536E-5 9 0.005868609840081675 0.0 5.1705813569001536E-5 10-11 0.007691239768388979 0.0 6.463226696125192E-5 12-13 0.010586765328253065 0.0 7.75587203535023E-5 14-15 0.015653935058015215 0.0 7.75587203535023E-5 16-17 0.024081982669762466 0.0 7.75587203535023E-5 18-19 0.034578262824269776 0.0 7.75587203535023E-5 20-21 0.045281366233053094 0.0 7.75587203535023E-5 22-23 0.057781246663359216 0.0 1.0341162713800307E-4 24-25 0.07212960992875714 0.0 1.0341162713800307E-4 26-27 0.08848157346995389 0.0 1.2926453392250384E-4 28-29 0.10545400677397863 0.0 1.2926453392250384E-4 30-31 0.12652412580334677 0.0 1.551174407070046E-4 32-33 0.1455647916501316 0.0 1.551174407070046E-4 34-35 0.16730708625589674 0.0 1.551174407070046E-4 36-37 0.19124687793834444 0.0 1.551174407070046E-4 38-39 0.2186121797697385 0.0 1.551174407070046E-4 40-41 0.24853691937279815 0.0 1.551174407070046E-4 42-43 0.2807108618661094 0.0 1.551174407070046E-4 44-45 0.3172539456060012 0.0 1.551174407070046E-4 46-47 0.3567571871727183 0.0 1.551174407070046E-4 48-49 0.40034518801138663 0.0 1.551174407070046E-4 50-51 0.44650555307511275 0.0 1.551174407070046E-4 52-53 0.496285325088669 0.0 1.551174407070046E-4 54-55 0.5507444732302198 0.0 1.551174407070046E-4 56-57 0.609702027152274 0.0 1.551174407070046E-4 58-59 0.6719816795961362 0.0 1.551174407070046E-4 60-61 0.7365622407438192 0.0 1.551174407070046E-4 62-63 0.8028490937392792 0.0 1.551174407070046E-4 64-65 0.8773313181854259 0.0 1.551174407070046E-4 66-67 0.9536232461064876 0.0 1.551174407070046E-4 68-69 1.0369471646729336 0.0 1.551174407070046E-4 70-71 1.1248729006470208 0.0 1.551174407070046E-4 72-73 1.2198435537198842 0.0 1.551174407070046E-4 74-75 1.3203467288446311 0.0 1.68043894099255E-4 76-77 1.4282179824029604 0.0 2.0682325427600614E-4 78-79 1.543573652475403 0.0 2.0682325427600614E-4 80-81 1.6687792800327401 0.0 2.0682325427600614E-4 82-83 1.8048819077997444 0.0 2.0682325427600614E-4 84-85 1.94868870178853 0.0 2.0682325427600614E-4 86-87 2.104025892203203 0.0 2.0682325427600614E-4 88-89 2.2714105371794533 0.0 2.0682325427600614E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACGC 20 0.002076317 71.23383 3 CGACTTA 25 0.005026943 56.986324 5 CGACGGG 130 0.0 51.240932 1 CGCTCGG 60 1.4758116E-6 47.580868 1 AGCGCGG 50 2.7999558E-5 47.580864 1 TGGGCGC 80 4.2346073E-9 47.49352 2 CGGCAGC 105 2.0008883E-11 45.31511 1 CGTGCGC 55 4.9159054E-5 43.255333 1 AACCGAG 220 0.0 41.092564 1 CGAGGTG 255 0.0 39.184242 1 AGTCGCC 85 3.5953963E-7 39.184242 1 GTGACGC 110 1.5952537E-9 38.9298 1 CGAGCGT 75 6.846143E-6 38.064693 1 GCGCGTT 50 0.001618195 37.990883 4 GTATGCG 90 5.627153E-7 37.00734 1 TCGCCGG 65 1.3298336E-4 36.533474 2 TTGTGTT 23990 0.0 36.474033 1 CGAGGGG 440 0.0 35.68565 1 GTCCGGC 95 8.716306E-7 34.995224 2 ACGTGCG 55 0.0025832506 34.537613 3 >>END_MODULE