FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_41442-1.34100000035721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_41442-1.34100000035721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059541
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2439397.973058703903625TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63970.20908365012921876No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT58480.1911397820784229TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC53240.17401303005908403No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC49750.1626060902599442No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG47280.154532983869149No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG43080.1408054345406713No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA34480.11269664305855029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG341950.079.6007163
CGGAAGA342200.079.4593054
GATCGGA344300.079.3031
GAAGAGC343150.079.129926
ATCGGAA346450.078.5830842
GGAAGAG352250.077.2609255
AGAGCAC427300.063.4798478
GAGCACA427850.063.3427479
AAGAGCA433950.062.44147
TCGGACG1050.058.793263
CGGTCGA250.00499027857.091531
CGTCCCG250.005027573656.9842344
TAACGGG352.7666663E-454.3728871
ATCGGAC1250.049.387952
GCCGTCT296600.045.05420350-51
ATGCCGT296150.045.0005248-49
CGTCTTC297300.044.9819252-53
TCGTATG300950.044.78954744-45
TCTCGTA291900.044.75371642-43
AATCTCG290100.044.67611740-41
Produced by FastQC (version 0.11.2)