##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_41442-1.34100000035721.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3059541 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69663750216127 33.0 33.0 33.0 33.0 33.0 2 32.713868518186224 33.0 33.0 33.0 33.0 33.0 3 32.7499974669403 33.0 33.0 33.0 33.0 33.0 4 36.6824709981007 37.0 37.0 37.0 37.0 37.0 5 36.653549339590484 37.0 37.0 37.0 37.0 37.0 6 36.54484153015109 37.0 37.0 37.0 37.0 37.0 7 36.42729775479394 37.0 37.0 37.0 37.0 37.0 8 36.384312875689524 37.0 37.0 37.0 37.0 37.0 9 36.42509807843726 37.0 37.0 37.0 37.0 37.0 10-11 36.357004204225404 37.0 37.0 37.0 37.0 37.0 12-13 36.33818520490492 37.0 37.0 37.0 37.0 37.0 14-15 38.16902306587818 40.0 37.0 40.0 37.0 40.0 16-17 38.21113248686649 40.0 37.0 40.0 37.0 40.0 18-19 38.17625045717642 40.0 37.0 40.0 37.0 40.0 20-21 38.14538014035438 40.0 37.0 40.0 37.0 40.0 22-23 37.925611390728214 40.0 37.0 40.0 33.0 40.0 24-25 38.003714119209384 40.0 37.0 40.0 35.0 40.0 26-27 37.94089603636624 40.0 37.0 40.0 33.0 40.0 28-29 37.716584775297996 40.0 37.0 40.0 33.0 40.0 30-31 37.675601993893856 40.0 37.0 40.0 33.0 40.0 32-33 37.54686389232894 40.0 37.0 40.0 33.0 40.0 34-35 37.550026131370686 40.0 37.0 40.0 33.0 40.0 36-37 37.46869791906695 40.0 37.0 40.0 33.0 40.0 38-39 37.18288168061811 40.0 37.0 40.0 33.0 40.0 40-41 37.03239587245277 38.5 37.0 40.0 33.0 40.0 42-43 36.82165592812778 37.0 37.0 40.0 33.0 40.0 44-45 36.641771102266645 37.0 37.0 40.0 33.0 40.0 46-47 36.77086938857822 37.0 37.0 40.0 33.0 40.0 48-49 36.36590096357591 37.0 37.0 40.0 33.0 40.0 50-51 36.30144064093274 37.0 37.0 40.0 33.0 40.0 52-53 36.30170489625732 37.0 37.0 40.0 33.0 40.0 54-55 35.89063964169789 37.0 37.0 40.0 30.0 40.0 56-57 35.72513393348872 37.0 37.0 40.0 27.0 40.0 58-59 35.57752404690769 37.0 35.0 40.0 27.0 40.0 60-61 35.21068879286142 37.0 33.0 40.0 27.0 40.0 62-63 34.99640550657762 37.0 33.0 37.0 27.0 40.0 64-65 34.70404237106154 37.0 33.0 37.0 27.0 40.0 66-67 33.96890824473344 37.0 33.0 37.0 27.0 40.0 68-69 33.86588821656582 37.0 33.0 37.0 27.0 40.0 70-71 33.56774610962886 37.0 33.0 37.0 27.0 38.5 72-73 32.73468013666103 37.0 33.0 37.0 22.0 37.0 74-75 31.722089849425124 35.0 33.0 37.0 18.5 37.0 76-77 29.148781140700518 33.0 27.0 33.0 15.0 37.0 78-79 31.177798565209617 33.0 33.0 37.0 15.0 37.0 80-81 31.45403150341832 35.0 33.0 37.0 15.0 37.0 82-83 31.376977298228724 37.0 33.0 37.0 15.0 37.0 84-85 31.225929804503355 33.0 33.0 37.0 15.0 37.0 86-87 31.065850727282296 33.0 33.0 37.0 15.0 37.0 88-89 30.93209471616821 33.0 33.0 37.0 15.0 37.0 90-91 30.78112059946247 33.0 33.0 37.0 15.0 37.0 92-93 30.622998515136747 33.0 33.0 37.0 15.0 37.0 94-95 30.54287146339925 33.0 33.0 37.0 15.0 37.0 96-97 30.40934604242924 33.0 27.0 37.0 15.0 37.0 98-99 30.12457849069517 33.0 27.0 37.0 15.0 37.0 100-101 28.631635268166043 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11548943168074999 2306 2 0.13594710720563796 2306 3 0.10928062635444746 2306 4 0.13580823250421048 2306 5 0.16657465731610444 2306 6 0.19850837270501387 2306 7 0.1892542190376787 2306 8 0.1558569763415818 2306 9 0.22674856131307308 2306 10-11 0.28455636611347046 2306 12-13 0.2556657218666345 2306 14-15 0.442205301139559 2306 16-17 0.5099022713229076 2306 18-19 0.5833949565861545 2306 20-21 0.6237807610692414 2306 22-23 0.5653157549506531 2306 24-25 0.49756944871181474 2306 26-27 0.48992634747684605 2306 28-29 0.5439478730824874 2306 30-31 0.5480735892981627 2306 32-33 0.5938426983834617 2306 34-35 0.6509678361943187 2306 36-37 0.7086139072924524 2306 38-39 0.7689327885167714 2306 40-41 0.6507022164056622 2306 42-43 0.5423161178084897 2306 44-45 0.7567286842913106 2306 46-47 0.6015005914936111 2306 48-49 0.49776451041061875 2306 50-51 0.55116901671758 2306 52-53 0.48786369386554185 2306 54-55 0.45186454532878884 2306 56-57 0.514812610646544 2306 58-59 0.4850210012297467 2306 60-61 0.4611187626222133 2306 62-63 0.3219472911964729 2306 64-65 0.4924769111887173 2306 66-67 0.2273043985084655 2306 68-69 0.6047216256933794 2306 70-71 0.5703487734946151 2306 72-73 0.037886791128443065 2306 74-75 0.12717307038213832 2306 76-77 -0.06441857470592538 2306 78-79 0.3149449585053077 2306 80-81 0.35661136624605305 2306 82-83 0.45066089589698777 2306 84-85 0.3861719455200152 2306 86-87 0.27350312988300374 2306 88-89 0.4426092103250632 2306 90-91 0.5724979244469957 2306 92-93 0.5269023083768545 2306 94-95 0.6849778637358206 2306 96-97 0.5417265953063577 2306 98-99 0.5223345131645978 2306 100-101 0.45446745743774386 2307 1 0.1457525456420612 2307 2 0.10498883684234528 2307 3 0.1469118508095022 2307 4 0.17851218290836357 2307 5 0.2324237580190882 2307 6 0.205510568195038 2307 7 0.2767235048931056 2307 8 0.25144125529745054 2307 9 0.2177824121456453 2307 10-11 0.26167928892319736 2307 12-13 0.22971476706658223 2307 14-15 0.5012506269790649 2307 16-17 0.5079864859631229 2307 18-19 0.44974087516965966 2307 20-21 0.49266523209627877 2307 22-23 0.5542451155655002 2307 24-25 0.5241542547823386 2307 26-27 0.5319174888853482 2307 28-29 0.5142619578817147 2307 30-31 0.5034355167314928 2307 32-33 0.6756396174721857 2307 34-35 0.5278546795109946 2307 36-37 0.5475764109940258 2307 38-39 0.6237489634378264 2307 40-41 0.5976381093012151 2307 42-43 0.5758422247629582 2307 44-45 0.6154779665560071 2307 46-47 0.67291080657062 2307 48-49 0.6329417780299593 2307 50-51 0.5268987530453089 2307 52-53 0.556924061356959 2307 54-55 0.4543656031159955 2307 56-57 0.7200382436357131 2307 58-59 0.5975319280483902 2307 60-61 0.5437471325570868 2307 62-63 0.35902868525327136 2307 64-65 0.2902169139364972 2307 66-67 0.4213166661031664 2307 68-69 0.49829728580459687 2307 70-71 0.3628185875594312 2307 72-73 0.2686599692576195 2307 74-75 0.0998821605647251 2307 76-77 0.06866337109705256 2307 78-79 0.27990579164204377 2307 80-81 0.3242195410663946 2307 82-83 0.39184833797869345 2307 84-85 0.33558166468230155 2307 86-87 0.42786425104438663 2307 88-89 0.34726508874823026 2307 90-91 0.482687275198284 2307 92-93 0.4540753700262812 2307 94-95 0.674786159305377 2307 96-97 0.43145946366945864 2307 98-99 0.42441489185515024 2307 100-101 0.21071072824166848 2201 1 0.14645203900748527 2201 2 0.08762447642565974 2201 3 0.09642149846195025 2201 4 0.11212908421059353 2201 5 0.15621647317595944 2201 6 0.15833664993527918 2201 7 0.15385647761779353 2201 8 0.1609015335008479 2201 9 0.22758538853812382 2201 10-11 0.1868250363463133 2201 12-13 0.18233967751429248 2201 14-15 0.3895291106059915 2201 16-17 0.3958861933970752 2201 18-19 0.3880861588061819 2201 20-21 0.41415679618486934 2201 22-23 0.3469906945103389 2201 24-25 0.4561726048606687 2201 26-27 0.5047691123098232 2201 28-29 0.30945126456703065 2201 30-31 0.513506400154121 2201 32-33 0.546645542491639 2201 34-35 0.5079552180244704 2201 36-37 0.577736036879692 2201 38-39 0.6747405300972886 2201 40-41 0.6249543428604483 2201 42-43 0.7251415316539394 2201 44-45 0.6688319569032188 2201 46-47 0.6386437727824088 2201 48-49 0.6414701339698894 2201 50-51 0.7320318774518597 2201 52-53 0.5934926746933087 2201 54-55 0.39022195438720786 2201 56-57 0.6045714919586374 2201 58-59 0.632484315366753 2201 60-61 0.6358562689473644 2201 62-63 0.5499114265988325 2201 64-65 0.5083470107043908 2201 66-67 0.475023830971935 2201 68-69 0.5785149278628055 2201 70-71 0.6622654843369205 2201 72-73 0.21381026285202154 2201 74-75 0.07450340579574188 2201 76-77 0.09952752498645268 2201 78-79 0.41494882387401333 2201 80-81 0.45105236365174406 2201 82-83 0.43827038121060014 2201 84-85 0.2531369985709446 2201 86-87 0.4370762465950513 2201 88-89 0.5744632118350701 2201 90-91 0.526043973538183 2201 92-93 0.5995115025328204 2201 94-95 0.48546961895457486 2201 96-97 0.5162771583449945 2201 98-99 0.5740142417655143 2201 100-101 0.44272903547200926 1101 1 0.12293679122733181 1101 2 0.13008886649956253 1101 3 0.13060911258200747 1101 4 0.14635653795939163 1101 5 0.20351132515126125 1101 6 0.14308589112066983 1101 7 0.06117200152008451 1101 8 0.193766807046444 1101 9 0.22616661372212832 1101 10-11 0.20966813001636098 1101 12-13 0.19555507809989336 1101 14-15 0.3903770058821294 1101 16-17 0.356118518005907 1101 18-19 0.39602792816229027 1101 20-21 0.3168563219815894 1101 22-23 0.4378522283434947 1101 24-25 0.39473310226773606 1101 26-27 0.45088722452204877 1101 28-29 0.3832818225303427 1101 30-31 0.5053695283170967 1101 32-33 0.5009403661696368 1101 34-35 0.5944027443931006 1101 36-37 0.5158783112682457 1101 38-39 0.4725206250273075 1101 40-41 0.6046221697166771 1101 42-43 0.6233846040265405 1101 44-45 0.681900379045409 1101 46-47 0.5641911394946533 1101 48-49 0.6807655049773587 1101 50-51 0.6805426013863212 1101 52-53 0.6605971304765319 1101 54-55 0.7292536577956952 1101 56-57 0.6517877816754023 1101 58-59 0.6576581204012939 1101 60-61 0.6095646272270088 1101 62-63 0.8321077108646406 1101 64-65 0.5709693220879544 1101 66-67 0.6131595523000613 1101 68-69 0.6494076855395647 1101 70-71 0.7036716110016243 1101 72-73 0.4763072469548888 1101 74-75 0.4061730622962294 1101 76-77 0.237690705871767 1101 78-79 0.7240783726660176 1101 80-81 0.6085259429035581 1101 82-83 0.7065291250782764 1101 84-85 0.5632665406609547 1101 86-87 0.7238630241573745 1101 88-89 0.6606358633820371 1101 90-91 0.5763725015420924 1101 92-93 0.7257176752178758 1101 94-95 0.6909814129740113 1101 96-97 0.6783163776669952 1101 98-99 0.7543627491570639 1101 100-101 0.6254843032389914 2304 1 0.08151578758633349 2304 2 0.0668610725312675 2304 3 0.0944634741716186 2304 4 0.14663136706840874 2304 5 0.16708711724865566 2304 6 0.13479524268427667 2304 7 0.13672827665411802 2304 8 0.10527040903546947 2304 9 0.11083367865055038 2304 10-11 0.1878181471686915 2304 12-13 0.16195975763989168 2304 14-15 0.26197837792593504 2304 16-17 0.28421169914482647 2304 18-19 0.2917070374808617 2304 20-21 0.2550233347227504 2304 22-23 0.4288645327793361 2304 24-25 0.383849210530812 2304 26-27 0.38818263891909766 2304 28-29 0.4233567335003201 2304 30-31 0.3885746377518018 2304 32-33 0.3982789588379845 2304 34-35 0.40152712353401654 2304 36-37 0.46139713626070744 2304 38-39 0.4890288063140602 2304 40-41 0.5217892462224754 2304 42-43 0.5456134876931387 2304 44-45 0.5117202457524073 2304 46-47 0.5454064006856143 2304 48-49 0.7958139928116736 2304 50-51 0.6656051138765235 2304 52-53 0.6686126826336789 2304 54-55 0.6424472895973565 2304 56-57 0.6191483962951736 2304 58-59 0.7063681512897375 2304 60-61 0.6399792861094014 2304 62-63 0.5731251805915818 2304 64-65 0.5697382907289281 2304 66-67 0.2707182077427319 2304 68-69 0.43136872570807583 2304 70-71 0.43837905425733936 2304 72-73 0.3265635878840527 2304 74-75 0.18100537735476152 2304 76-77 0.276262339019258 2304 78-79 0.583752286057976 2304 80-81 0.4147736474660064 2304 82-83 0.5516863440712996 2304 84-85 0.3262741685491122 2304 86-87 0.5168764514206359 2304 88-89 0.4825685977553391 2304 90-91 0.3892072881033606 2304 92-93 0.4875307850116606 2304 94-95 0.5437749805123282 2304 96-97 0.4560538127401763 2304 98-99 0.575541974427523 2304 100-101 0.5233356846337571 2202 1 0.08889758842582296 2202 2 0.07977527440120724 2202 3 0.11043664059489089 2202 4 0.09651737176250919 2202 5 0.148646438747015 2202 6 0.15659456157590057 2202 7 0.13620544846464355 2202 8 0.12738026516144885 2202 9 0.11567736832659392 2202 10-11 0.11067136722751769 2202 12-13 0.13533476911841547 2202 14-15 0.1419964670901095 2202 16-17 0.164094267687652 2202 18-19 0.15566210684075799 2202 20-21 0.16815338218706444 2202 22-23 0.22856480757270248 2202 24-25 0.27498122489551236 2202 26-27 0.1670477907170138 2202 28-29 0.18661158829824842 2202 30-31 0.16186024017923017 2202 32-33 0.21429626203192953 2202 34-35 0.2084625702978613 2202 36-37 0.24411734369365234 2202 38-39 0.22416893315544684 2202 40-41 0.16888169942713205 2202 42-43 0.1699473631089603 2202 44-45 0.2441620558142077 2202 46-47 0.29450959482519323 2202 48-49 0.2704091835822595 2202 50-51 0.3388093545611497 2202 52-53 0.4468073899733227 2202 54-55 0.4211455708305394 2202 56-57 0.4874304983903386 2202 58-59 0.527869226074408 2202 60-61 0.42783693465629824 2202 62-63 0.41275318759421964 2202 64-65 0.3516199488891516 2202 66-67 0.3598473310008359 2202 68-69 0.3778099393804055 2202 70-71 0.3964594898191365 2202 72-73 0.36626569924102625 2202 74-75 0.1666258741945903 2202 76-77 0.31624388451716 2202 78-79 0.5139710148597381 2202 80-81 0.5969032417138074 2202 82-83 0.6128809398347599 2202 84-85 0.4921911519242599 2202 86-87 0.4764582283650469 2202 88-89 0.54563851257236 2202 90-91 0.5414949453097471 2202 92-93 0.5073454303228431 2202 94-95 0.6068863661205839 2202 96-97 0.4914567854059868 2202 98-99 0.5416573757907273 2202 100-101 0.4107726021295939 1102 1 0.04833819699231867 1102 2 0.020951695794700242 1102 3 0.008158781416213401 1102 4 0.022697071493553267 1102 5 0.06615777911873266 1102 6 0.022667782241470036 1102 7 0.05395430104650245 1102 8 0.017039953652478346 1102 9 0.06052895664768698 1102 10-11 0.07176787078016389 1102 12-13 0.07532237499533778 1102 14-15 0.059665452009760145 1102 16-17 0.07564438610923219 1102 18-19 0.12231213092650961 1102 20-21 0.1568391451699469 1102 22-23 0.17524561625159407 1102 24-25 0.21724652268898126 1102 26-27 0.11657059188917884 1102 28-29 0.05433464069811578 1102 30-31 0.08381698954028138 1102 32-33 0.05805190712846553 1102 34-35 0.0032168956962763673 1102 36-37 0.15017122885817713 1102 38-39 0.11895060591044881 1102 40-41 0.05989412031214414 1102 42-43 -0.029111494758389256 1102 44-45 0.13233541129304882 1102 46-47 0.18332920931946006 1102 48-49 0.15973560229955552 1102 50-51 0.24676271017379747 1102 52-53 0.20332622201539152 1102 54-55 0.2357946799924946 1102 56-57 0.22061835549496323 1102 58-59 0.19512570079106695 1102 60-61 0.05271399699823576 1102 62-63 0.22971929527087553 1102 64-65 0.26901587839819996 1102 66-67 0.13812529220940917 1102 68-69 0.1367695439058494 1102 70-71 0.07538605171281176 1102 72-73 -0.19169459774959563 1102 74-75 -0.2767667702864287 1102 76-77 -0.3873580216019583 1102 78-79 -0.05517681809223873 1102 80-81 -0.0691466458860468 1102 82-83 -0.01590545640251051 1102 84-85 -0.06683732277901555 1102 86-87 -0.11230547876567343 1102 88-89 -0.1182451214982656 1102 90-91 -0.03402954688245785 1102 92-93 0.019739553401002752 1102 94-95 8.959151150023104E-4 1102 96-97 -0.03367392994064389 1102 98-99 -0.07006514806085207 1102 100-101 -0.04693454364268845 2305 1 0.061469991366251975 2305 2 0.040641896314873804 2305 3 0.05209710617980079 2305 4 0.1016174327414987 2305 5 0.05449722514129007 2305 6 0.02707551059835822 2305 7 0.10765457551823943 2305 8 0.09333845065006585 2305 9 -0.011585419558372223 2305 10-11 0.017303510155471713 2305 12-13 -0.002718912624672498 2305 14-15 0.08179660814702316 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-0.6003429562318914 1213 66-67 -0.5596817867467294 1213 68-69 -0.14917601355028154 1213 70-71 -0.023924381415667995 1213 72-73 0.27577204343595696 1213 74-75 0.2944068999021816 1213 76-77 -2.286538273991127 1213 78-79 -2.0874898977245024 1213 80-81 -1.5034075800589939 1213 82-83 -1.0197140629640238 1213 84-85 -0.8191634204852107 1213 86-87 -0.6993277394198074 1213 88-89 -0.7866082689960727 1213 90-91 -1.8840058633225674 1213 92-93 -1.021155111206994 1213 94-95 -0.6771831684226868 1213 96-97 -0.6093367542571961 1213 98-99 -2.383022578202233 1213 100-101 -1.0511293089136977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 13.0 10 20.0 11 29.0 12 39.0 13 121.0 14 412.0 15 1095.0 16 2448.0 17 4082.0 18 5387.0 19 6311.0 20 8107.0 21 10622.0 22 14126.0 23 18457.0 24 23985.0 25 31200.0 26 42066.0 27 62561.0 28 98509.0 29 122161.0 30 84947.0 31 67613.0 32 87751.0 33 124727.0 34 191632.0 35 330254.0 36 627938.0 37 895789.0 38 196953.0 39 180.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.96864042820767 23.60312079782098 21.822600147533624 13.605638626437724 2 27.731567904434634 29.134022215822895 30.466657797683233 12.667752082059232 3 34.26240792601135 17.684089144094322 32.7539241757466 15.299578754147742 4 27.253728582163138 17.413429007815225 30.781545336375622 24.551297073646015 5 36.727385758256496 16.20526759446563 31.488096580518576 15.579250066759295 6 32.30713365174711 16.980324826501754 35.04153073941483 15.671010782336307 7 30.085231739009217 26.489006030643157 26.40615046505342 17.019611765294208 8 26.876253660271267 33.95829635883291 22.78845094738067 16.37699903351516 9 35.15112234155385 23.354091348996466 23.792621180758815 17.702165128690872 10-11 26.570990876388368 29.979745281470972 26.999438476578458 16.449825365562205 12-13 21.824417453467692 28.66055725352267 26.9462641618465 22.56876113116314 14-15 20.9773622906181 29.254878427842606 27.82716427071904 21.940595010820253 16-17 24.757553502306394 25.493971154496702 28.411941529791562 21.336533813405346 18-19 19.46765827591318 25.584103615626997 33.13065025983494 21.817587848624882 20-21 24.266352371156326 25.872263192420043 32.55856025462643 17.302824181797206 22-23 19.55711657402205 34.77454624729657 28.176317950960616 17.49201922772076 24-25 19.947959514188565 25.196017964786222 32.73334137375508 22.12268114727013 26-27 19.771560505317627 25.351220983801166 27.996241919948123 26.880976590933088 28-29 23.880232361651633 30.224043410433133 27.748214519759664 18.14750970815557 30-31 18.79649594497998 26.365899329343844 32.06990852549451 22.76769620018166 32-33 18.470368594504862 30.973387838241095 28.274077712964136 22.28216585428991 34-35 18.88237483988611 26.366340572000833 32.84777030280032 21.90351428531273 36-37 23.52964440248462 27.017324987199874 28.130988950791796 21.32204165952371 38-39 24.093118056646183 31.315586957562676 27.247551139707316 17.343743846083825 40-41 23.882699321695195 30.940870503693528 27.14328858040378 18.033141594207493 42-43 19.00796584018671 31.006472786498062 32.21423490068562 17.77132647262961 44-45 18.632213996896898 26.536081955312614 32.61262138746556 22.219082660324933 46-47 23.645973766137534 26.12285909517542 27.901446263466635 22.32972087522041 48-49 19.36010326155437 31.362395856332686 31.456368054616966 17.821132827495973 50-51 23.907695209311804 26.56948102008319 31.134741793015035 18.38808197758997 52-53 18.95866642413652 26.544695576456075 26.78967717178472 27.70696082762268 54-55 23.881697279407486 26.52442034334478 31.154465267021603 18.439417110226135 56-57 19.29013664809051 26.588249869206365 31.0065822581233 23.11503122457982 58-59 19.18331900654292 26.482528589424913 31.641758646622563 22.692393757409597 60-61 23.2987879421821 26.755898485254605 31.65041545994783 18.294898112615467 62-63 18.88220359273608 26.803971735987915 31.291124717772544 23.022699953503462 64-65 23.52399410558688 26.627942630153033 31.132991061621983 18.7150722026381 66-67 19.430651999140792 35.33380369504214 26.38760245978685 18.84794184603021 68-69 19.50175710135574 35.718256967225244 26.31135244247469 18.468633488944327 70-71 18.84951261562566 36.58472588736391 26.23454741571682 18.331214081293616 72-73 18.761884460161067 36.331922673522925 26.171085222858963 18.73510764345704 74-75 19.50854280984667 34.99658496856504 26.123192232103126 19.37167998948517 76-77 19.81956724059603 33.84876520151556 26.52875861666474 19.802908941223667 78-79 19.62214536061799 33.36195893245367 27.14393287945359 19.871962827474757 80-81 19.081541133702594 33.314909614827705 27.134198757820823 20.469350493648882 82-83 18.538875602582216 32.78973872224625 27.29154471209897 21.379840963072567 84-85 18.734624028330114 32.01900593898821 27.79872715187607 21.447642880805606 86-87 18.847605598393212 31.939450834902456 28.360218591097304 20.85272497560703 88-89 19.205287404196696 32.33268197601562 27.816313967086458 20.645716652701225 90-91 19.70871920075873 31.709042203137766 27.427053933878014 21.155184662225494 92-93 19.12240884731888 32.21455817435198 27.459691545732177 21.203341432596968 94-95 18.925609823679682 32.58109608304035 27.429964498573618 21.063329594706353 96-97 19.366896327593935 32.95968340729499 26.792056541511105 20.881363723599975 98-99 19.871784915951324 32.00317227130146 26.451877327726002 21.67316548502122 100-101 19.758930620587652 32.094688476911784 26.134746069140753 22.011634833359814 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 871.0 1 578.0 2 300.0 3 417.5 4 465.5 5 389.0 6 373.5 7 372.5 8 397.0 9 420.0 10 424.5 11 426.5 12 388.5 13 411.5 14 455.0 15 448.0 16 479.5 17 507.0 18 520.5 19 586.5 20 659.0 21 749.5 22 899.5 23 1153.0 24 1507.0 25 2092.5 26 3377.5 27 5487.0 28 7626.0 29 9717.5 30 12144.0 31 14317.5 32 17549.5 33 26769.0 34 43930.5 35 74545.0 36 114112.5 37 157286.5 38 205827.5 39 237673.0 40 248405.5 41 236169.0 42 213859.0 43 207816.5 44 197852.0 45 179390.5 46 168890.0 47 149620.0 48 116273.5 49 89691.5 50 70911.5 51 57867.5 52 47923.0 53 37061.5 54 27769.5 55 20005.5 56 13545.5 57 8888.0 58 6058.5 59 4301.5 60 3100.5 61 2255.0 62 1595.0 63 1113.5 64 812.0 65 561.5 66 412.0 67 324.0 68 228.5 69 170.0 70 128.0 71 83.0 72 54.0 73 44.0 74 36.5 75 29.5 76 18.5 77 14.0 78 12.5 79 9.0 80 6.0 81 3.5 82 3.5 83 3.0 84 1.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17394766077656745 2 0.006210081839073247 3 0.001470808856622611 4 0.0 5 0.0013727549328477703 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.287924888079617E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.171160314570061E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.634232062914012E-5 38-39 1.7976552692054135E-4 40-41 6.536928251656048E-5 42-43 0.0012910433297020696 44-45 6.536928251656049E-4 46-47 0.005703469899569903 48-49 0.004101922477914171 50-51 0.007893340863874679 52-53 0.0041672917604307315 54-55 0.018793668723511143 56-57 0.010867643218378182 58-59 0.019169542097981364 60-61 0.009184384193576749 62-63 0.005670785258311622 64-65 0.012191371189338532 66-67 0.02948154641496878 68-69 0.031132120798511933 70-71 0.03503793542887643 72-73 0.030233293163909224 74-75 0.03281537982331337 76-77 0.032864406785200785 78-79 0.016587455438577224 80-81 0.0025820866594041393 82-83 0.0 84-85 0.005932262388377864 86-87 0.02518351608950493 88-89 0.026147713006624197 90-91 0.02418663453112738 92-93 0.03374689209917436 94-95 0.03044574333208805 96-97 0.03183484058556496 98-99 0.038061264745267345 100-101 0.025984289800332794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3059541.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.299833025700984 #Duplication Level Percentage of deduplicated Percentage of total 1 85.57456264873797 31.91916898045314 2 6.5496328727031266 4.8860042506293775 3 2.0910611338819454 2.339886934209886 4 1.1847546444210144 1.7676460165331036 5 0.8864601478683863 1.6532407749714508 6 0.5560671655461779 1.2444727455568356 7 0.40224658931622864 1.0502611431657132 8 0.30642982404024083 0.9143825016636733 9 0.23176810810439022 0.7780420559678735 >10 1.7623383028979496 12.852473170032003 >50 0.23340996566201747 6.030317881418846 >100 0.1867474674256215 15.005623662154443 >500 0.027664907602285682 7.015994137661799 >1k 0.006504305430458731 3.9608251658209963 >5k 2.639372719937614E-4 0.5765452509872835 >10k+ 8.797909014473947E-5 8.005115328773568 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 243939 7.973058703903625 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6397 0.20908365012921876 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5848 0.1911397820784229 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5324 0.17401303005908403 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 4975 0.1626060902599442 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4728 0.154532983869149 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4308 0.1408054345406713 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3448 0.11269664305855029 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004804642264967196 0.0 0.0 2 0.005458335090132801 0.0 0.0 3 0.005915920067748724 0.0 0.0 4 0.006340820404106368 0.0 0.0 5 0.007354044283113055 0.0 0.0 6 0.008269214238344902 0.0 0.0 7 0.010099554148808596 0.0 0.0 8 0.01199526334178885 0.0 0.0 9 0.013662180045961142 0.0 0.0 10-11 0.021718944116127223 0.0 0.0 12-13 0.03126285936354506 0.0 0.0 14-15 0.04342154591162531 0.0 0.0 16-17 0.05729617612576527 0.0 0.0 18-19 0.07425950493881271 0.0 0.0 20-21 0.08499640959215778 0.0 0.0 22-23 0.09576599888676111 0.0 0.0 24-25 0.1070422001208678 0.0 0.0 26-27 0.1188086709738487 0.0 0.0 28-29 0.1325852472642138 0.0 0.0 30-31 0.22933178538872334 0.0 0.0 32-33 0.24734102272203576 0.0 0.0 34-35 0.2607744102791889 0.0 0.0 36-37 0.2750412561884283 0.0 0.0 38-39 0.2883602475011775 0.0 0.0 40-41 0.3038527674576023 0.0 0.0 42-43 0.31996629559793444 0.0 0.0 44-45 0.3381226138169091 0.0 3.268464125828025E-5 46-47 0.357586317686215 0.0 3.268464125828025E-5 48-49 0.3794850273292628 0.0 3.268464125828025E-5 50-51 0.40113860216287345 0.0 3.268464125828025E-5 52-53 0.42563574078595445 0.0 3.268464125828025E-5 54-55 0.4503453295772144 0.0 3.268464125828025E-5 56-57 0.47724478933277903 0.0 3.268464125828025E-5 58-59 0.5073473439316551 0.0 3.268464125828025E-5 60-61 0.5414537670846705 0.0 3.268464125828025E-5 62-63 0.5777664035226199 0.0 3.268464125828025E-5 64-65 0.6167755228643774 0.0 3.268464125828025E-5 66-67 0.6586608906368635 0.0 3.268464125828025E-5 68-69 0.7041252266271314 0.0 3.268464125828025E-5 70-71 0.751795775902333 0.0 3.268464125828025E-5 72-73 0.8041892558393562 0.0 3.268464125828025E-5 74-75 0.8603251272004526 0.0 3.268464125828025E-5 76-77 0.9235862503558541 0.0 4.902696188742037E-5 78-79 0.9911780884779775 0.0 6.53692825165605E-5 80-81 1.0658624937531478 0.0 6.53692825165605E-5 82-83 1.1461849996453717 0.0 6.53692825165605E-5 84-85 1.2318841290245826 0.0 8.171160314570061E-5 86-87 1.3260322381690588 0.0 9.805392377484074E-5 88-89 1.4295935239959197 0.0 9.805392377484074E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 34195 0.0 79.600716 3 CGGAAGA 34220 0.0 79.459305 4 GATCGGA 34430 0.0 79.303 1 GAAGAGC 34315 0.0 79.12992 6 ATCGGAA 34645 0.0 78.583084 2 GGAAGAG 35225 0.0 77.260925 5 AGAGCAC 42730 0.0 63.479847 8 GAGCACA 42785 0.0 63.342747 9 AAGAGCA 43395 0.0 62.4414 7 TCGGACG 105 0.0 58.79326 3 CGGTCGA 25 0.004990278 57.09153 1 CGTCCCG 25 0.0050275736 56.984234 4 TAACGGG 35 2.7666663E-4 54.372887 1 ATCGGAC 125 0.0 49.38795 2 GCCGTCT 29660 0.0 45.054203 50-51 ATGCCGT 29615 0.0 45.00052 48-49 CGTCTTC 29730 0.0 44.98192 52-53 TCGTATG 30095 0.0 44.789547 44-45 TCTCGTA 29190 0.0 44.753716 42-43 AATCTCG 29010 0.0 44.676117 40-41 >>END_MODULE