##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_40825-1.341000000356f9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1820722 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66596163499974 33.0 33.0 33.0 33.0 33.0 2 32.68002528667199 33.0 33.0 33.0 33.0 33.0 3 32.714133184527896 33.0 33.0 33.0 33.0 33.0 4 36.65375548820742 37.0 37.0 37.0 37.0 37.0 5 36.62325275357798 37.0 37.0 37.0 37.0 37.0 6 36.50530009523695 37.0 37.0 37.0 37.0 37.0 7 36.4199658157588 37.0 37.0 37.0 37.0 37.0 8 36.337153612687715 37.0 37.0 37.0 37.0 37.0 9 36.3844348560626 37.0 37.0 37.0 37.0 37.0 10-11 36.30686425494941 37.0 37.0 37.0 37.0 37.0 12-13 36.2887480900434 37.0 37.0 37.0 35.0 37.0 14-15 38.08427096503475 40.0 37.0 40.0 35.0 40.0 16-17 38.022167305058105 40.0 37.0 40.0 37.0 40.0 18-19 37.92935906744687 40.0 37.0 40.0 33.0 40.0 20-21 37.854530510423885 40.0 37.0 40.0 33.0 40.0 22-23 37.655668190970395 40.0 37.0 40.0 33.0 40.0 24-25 37.62324808510031 40.0 37.0 40.0 33.0 40.0 26-27 37.48871436715764 40.0 37.0 40.0 33.0 40.0 28-29 37.277908159510346 40.0 37.0 40.0 33.0 40.0 30-31 37.15556713215966 40.0 37.0 40.0 33.0 40.0 32-33 36.99855359577135 37.0 37.0 40.0 33.0 40.0 34-35 36.8098847600018 37.0 37.0 40.0 33.0 40.0 36-37 36.6912483069903 37.0 37.0 40.0 33.0 40.0 38-39 36.55875306609136 37.0 37.0 40.0 33.0 40.0 40-41 36.42062379649391 37.0 37.0 40.0 33.0 40.0 42-43 36.07414860698118 37.0 37.0 40.0 33.0 40.0 44-45 35.883062598243995 37.0 37.0 40.0 33.0 40.0 46-47 35.838814492272846 37.0 37.0 40.0 30.0 40.0 48-49 35.53206640003251 37.0 35.0 40.0 27.0 40.0 50-51 35.341287412356195 37.0 33.0 40.0 27.0 40.0 52-53 35.15744221248494 37.0 33.0 40.0 27.0 40.0 54-55 34.83308434785761 37.0 33.0 40.0 27.0 40.0 56-57 34.580461761872485 37.0 33.0 37.0 27.0 40.0 58-59 34.34176579400919 37.0 33.0 37.0 27.0 40.0 60-61 34.055000983126476 37.0 33.0 37.0 27.0 40.0 62-63 33.75074832950884 37.0 33.0 37.0 27.0 40.0 64-65 33.436254683581566 37.0 33.0 37.0 27.0 40.0 66-67 32.94820928181238 37.0 33.0 37.0 22.0 37.0 68-69 32.663401661538664 37.0 33.0 37.0 22.0 37.0 70-71 32.35090914483375 33.0 33.0 37.0 22.0 37.0 72-73 31.746724650990103 33.0 33.0 37.0 22.0 37.0 74-75 31.152300021639768 33.0 30.0 37.0 15.0 37.0 76-77 28.35472878341669 30.0 24.5 33.0 15.0 35.0 78-79 30.90470758303574 33.0 30.0 37.0 15.0 37.0 80-81 31.406116914059368 33.0 33.0 37.0 15.0 37.0 82-83 31.331580274198917 33.0 33.0 37.0 15.0 37.0 84-85 31.238472704784144 33.0 33.0 37.0 15.0 37.0 86-87 31.076251069630622 33.0 33.0 37.0 15.0 37.0 88-89 30.913864390060645 33.0 33.0 37.0 15.0 37.0 90-91 30.765339519157784 33.0 30.0 37.0 15.0 37.0 92-93 30.59855458439015 33.0 27.0 37.0 15.0 37.0 94-95 30.47906929229174 33.0 27.0 37.0 15.0 37.0 96-97 30.32045693960967 33.0 27.0 37.0 15.0 37.0 98-99 30.001621609449437 33.0 27.0 37.0 15.0 37.0 100-101 28.224717996487108 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14393800982421823 2306 2 0.15961437706237547 2306 3 0.18277406486020453 2306 4 0.16043433212247038 2306 5 0.21367259130663285 2306 6 0.26430943874294854 2306 7 0.28165481792058955 2306 8 0.32566147881846064 2306 9 0.3131179377056341 2306 10-11 0.3002609787437791 2306 12-13 0.30721068510318617 2306 14-15 0.561078309170675 2306 16-17 0.6277054711960375 2306 18-19 0.690617712746608 2306 20-21 0.6750114400951119 2306 22-23 0.7991531793014346 2306 24-25 0.7955678133591988 2306 26-27 0.774558770490394 2306 28-29 0.7279866809594822 2306 30-31 0.7815395634007061 2306 32-33 0.8871772275896888 2306 34-35 0.9067283097536887 2306 36-37 0.7852691883303677 2306 38-39 0.8002122606792881 2306 40-41 1.0977835136315335 2306 42-43 1.1409127015494036 2306 44-45 1.0876661972028927 2306 46-47 0.8929160620969796 2306 48-49 1.124971490480604 2306 50-51 1.1199182059346313 2306 52-53 1.064183213220602 2306 54-55 1.1217944814888199 2306 56-57 0.910223017144709 2306 58-59 0.9068055920047087 2306 60-61 0.8696103445049417 2306 62-63 0.9658290491542942 2306 64-65 1.2308499810827698 2306 66-67 1.060944798157081 2306 68-69 1.0960974802176011 2306 70-71 1.1330034146565353 2306 72-73 0.8721602334862695 2306 74-75 0.8340612916116008 2306 76-77 0.2067720521522709 2306 78-79 0.8930467251561716 2306 80-81 1.0117344754394004 2306 82-83 1.11904685343956 2306 84-85 1.088277627608175 2306 86-87 1.1440974272686866 2306 88-89 1.1928203478630621 2306 90-91 1.2512298979783694 2306 92-93 1.218934616234293 2306 94-95 1.287274468702087 2306 96-97 1.1666079016247046 2306 98-99 1.1863208739875866 2306 100-101 0.932365893488047 2307 1 0.10807055347785166 2307 2 0.10661733836126785 2307 3 0.11214502240478197 2307 4 0.14480006448449245 2307 5 0.20959727480830992 2307 6 0.24333701162935029 2307 7 0.18735493806720172 2307 8 0.2373249336092229 2307 9 0.27680672105283577 2307 10-11 0.2494580278735299 2307 12-13 0.27113194018986064 2307 14-15 0.4919220403638249 2307 16-17 0.4461167984358525 2307 18-19 0.46421603851555204 2307 20-21 0.6157857339350343 2307 22-23 0.5445116203068352 2307 24-25 0.5583165526888507 2307 26-27 0.579346330427903 2307 28-29 0.6367561884887465 2307 30-31 0.6260099949508131 2307 32-33 0.5988150453101184 2307 34-35 0.6920781362689397 2307 36-37 0.7812085371053286 2307 38-39 0.7111913360501063 2307 40-41 0.7082380067453684 2307 42-43 0.7020514111357059 2307 44-45 0.8041522456395711 2307 46-47 0.7251716788966505 2307 48-49 0.7700957759558946 2307 50-51 0.7472575886026647 2307 52-53 0.8692347480772398 2307 54-55 0.8070646810017124 2307 56-57 0.8452338585837751 2307 58-59 0.8029773582033144 2307 60-61 0.7817512354148519 2307 62-63 0.7063235078536252 2307 64-65 0.606808383849831 2307 66-67 0.7305888774060421 2307 68-69 0.9378160003937595 2307 70-71 0.9170311939430889 2307 72-73 0.6381434803125927 2307 74-75 0.43417405126841047 2307 76-77 0.25869037852955756 2307 78-79 0.6224387572416177 2307 80-81 0.5158619351316212 2307 82-83 0.48368257891433686 2307 84-85 0.4808428228952941 2307 86-87 0.4559493036632496 2307 88-89 0.35279159306426067 2307 90-91 0.5461596784772489 2307 92-93 0.5934881401365146 2307 94-95 0.586765149192491 2307 96-97 0.7109662796237508 2307 98-99 0.5573655954668872 2307 100-101 0.447809512573329 2201 1 0.0847087511104263 2201 2 0.11759179483360782 2201 3 0.09757889883591275 2201 4 0.13751995031358888 2201 5 0.2044605669487396 2201 6 0.1616114786639784 2201 7 0.2507050482342592 2201 8 0.2511364681122714 2201 9 0.2032755537241826 2201 10-11 0.2993112612827673 2201 12-13 0.18392654802215702 2201 14-15 0.3697464068111387 2201 16-17 0.384369944399225 2201 18-19 0.5020589749281612 2201 20-21 0.4925406079368386 2201 22-23 0.5482290522476774 2201 24-25 0.45430902644895355 2201 26-27 0.5835621546911867 2201 28-29 0.4975534196970912 2201 30-31 0.6325151323955751 2201 32-33 0.7191260676653073 2201 34-35 0.6894088265754661 2201 36-37 0.6994608970384206 2201 38-39 0.7501224331273022 2201 40-41 0.7394478301587597 2201 42-43 0.7876488801182191 2201 44-45 0.8143841491347033 2201 46-47 0.7123692107615724 2201 48-49 0.746289866482023 2201 50-51 0.7490246829189289 2201 52-53 0.6813953733643245 2201 54-55 0.837902859474795 2201 56-57 0.679569626154624 2201 58-59 0.6166466703531412 2201 60-61 0.8043913637342968 2201 62-63 0.9613724013931915 2201 64-65 0.7706524649190314 2201 66-67 0.9543879091149918 2201 68-69 0.9319681807680595 2201 70-71 1.0128554080970318 2201 72-73 0.9713807705714714 2201 74-75 0.8612341260886645 2201 76-77 0.5039905315598254 2201 78-79 0.9822141490899945 2201 80-81 1.044671418586912 2201 82-83 0.9963575627638726 2201 84-85 0.9393902880704665 2201 86-87 1.0307494186201787 2201 88-89 1.2139081490726085 2201 90-91 1.2826698023015837 2201 92-93 1.3367092430843748 2201 94-95 1.2866224355703437 2201 96-97 1.1577930508324492 2201 98-99 1.3380471819707473 2201 100-101 1.042784322759296 1101 1 0.07564475192700115 1101 2 0.07078835160268682 1101 3 0.09712297695506322 1101 4 0.16431046441655184 1101 5 0.1281855167633097 1101 6 0.0948971783679724 1101 7 0.05652315581821199 1101 8 0.22991228383817486 1101 9 0.06310339217813521 1101 10-11 0.1093381402593181 1101 12-13 0.15882022295666332 1101 14-15 0.3281690531994883 1101 16-17 0.3686576515204507 1101 18-19 0.3053184761360157 1101 20-21 0.35320543730637866 1101 22-23 0.5783096891773454 1101 24-25 0.3941205333728419 1101 26-27 0.4665875706348359 1101 28-29 0.34726251431789024 1101 30-31 0.41375088483679434 1101 32-33 0.4802400141115015 1101 34-35 0.5348819305868986 1101 36-37 0.48637866097976 1101 38-39 0.7034448787739223 1101 40-41 0.7622643386137256 1101 42-43 0.6667513264453291 1101 44-45 0.6827111880241645 1101 46-47 0.6357232987476991 1101 48-49 0.6492805779243014 1101 50-51 0.7484156529437627 1101 52-53 0.664784846332239 1101 54-55 0.7368957484544154 1101 56-57 0.8530036705899917 1101 58-59 0.7456589530247015 1101 60-61 0.6045376670244238 1101 62-63 0.46230125716536463 1101 64-65 0.6048942396119656 1101 66-67 0.7421494687761125 1101 68-69 0.7969744752119325 1101 70-71 0.6538441461460991 1101 72-73 0.7158637755729984 1101 74-75 0.5872999285451961 1101 76-77 0.3639680757059942 1101 78-79 0.7651885290022129 1101 80-81 0.701416680741989 1101 82-83 0.7088273664452664 1101 84-85 0.5986839493953617 1101 86-87 0.7876886176947231 1101 88-89 0.9127309179474281 1101 90-91 0.8906926664436625 1101 92-93 0.7762893866657414 1101 94-95 0.8797155593157058 1101 96-97 0.9407095899090336 1101 98-99 0.8068403499473398 1101 100-101 0.852239598183747 2304 1 0.0921329227606762 2304 2 0.08741089452067285 2304 3 0.12134659906200795 2304 4 0.11558647268106625 2304 5 0.2035846839933697 2304 6 0.17075759146735692 2304 7 0.1532280911490176 2304 8 0.11561934848209887 2304 9 0.08282870867915193 2304 10-11 0.1908169071532413 2304 12-13 0.19445578396011598 2304 14-15 0.3778208020374265 2304 16-17 0.34112316865289216 2304 18-19 0.40370832788442357 2304 20-21 0.4058527569202752 2304 22-23 0.4811590934540817 2304 24-25 0.5369426841265224 2304 26-27 0.6332085339436802 2304 28-29 0.5999852339414815 2304 30-31 0.6789103937679144 2304 32-33 0.7281434515516025 2304 34-35 0.6734747305178175 2304 36-37 0.8492822774422351 2304 38-39 0.9303580487124918 2304 40-41 0.862182409552922 2304 42-43 0.8208344054615893 2304 44-45 0.9283783201489868 2304 46-47 0.8873396601285677 2304 48-49 0.8247142859409209 2304 50-51 0.8798123854695987 2304 52-53 1.0699885000734923 2304 54-55 0.8907332509028123 2304 56-57 0.9316254510832991 2304 58-59 0.9970849188503834 2304 60-61 1.1348617396358947 2304 62-63 0.9981476433355709 2304 64-65 0.8226216664708019 2304 66-67 0.967272204406413 2304 68-69 1.2303968985008495 2304 70-71 1.099400495016468 2304 72-73 1.006733634735049 2304 74-75 0.6154868952954793 2304 76-77 0.45347989223544616 2304 78-79 0.8064972035837705 2304 80-81 0.561652232057174 2304 82-83 0.5298384847874829 2304 84-85 0.5593748493724071 2304 86-87 0.6710115256077209 2304 88-89 0.7106561539936891 2304 90-91 0.6207395703044405 2304 92-93 0.7751474345637028 2304 94-95 0.7487798620446391 2304 96-97 0.5130714846817916 2304 98-99 0.5313127078404385 2304 100-101 0.4169001612406902 2202 1 0.1408569475086523 2202 2 0.06735608857079711 2202 3 0.07559704550408242 2202 4 0.1265158708624199 2202 5 0.16986832416920095 2202 6 0.14171695147529562 2202 7 0.12112380786397381 2202 8 0.10090827772674515 2202 9 0.15471421790226003 2202 10-11 0.15991198865266654 2202 12-13 0.21843047924353698 2202 14-15 0.17402920422066614 2202 16-17 0.19834738841638 2202 18-19 0.20303290736042356 2202 20-21 0.32949158353947183 2202 22-23 0.2691361351269279 2202 24-25 0.3313296491811428 2202 26-27 0.31999035927336905 2202 28-29 0.21410943594808884 2202 30-31 0.29898722135015987 2202 32-33 0.26887532964791205 2202 34-35 0.3070558976798381 2202 36-37 0.2603150764925175 2202 38-39 0.34382078756772216 2202 40-41 0.3868890914899836 2202 42-43 0.3768143254548235 2202 44-45 0.3225701921505717 2202 46-47 0.35708220863625684 2202 48-49 0.34411193226264913 2202 50-51 0.404717959966689 2202 52-53 0.6217608537750294 2202 54-55 0.53395973796394 2202 56-57 0.7252434036763091 2202 58-59 0.7547657385513702 2202 60-61 0.8619838612185688 2202 62-63 0.5569706025932248 2202 64-65 0.4598623791962737 2202 66-67 0.4331254922593857 2202 68-69 0.4603151470676963 2202 70-71 0.48272383848957645 2202 72-73 0.200469720979207 2202 74-75 0.24773707189501337 2202 76-77 0.08061469176492508 2202 78-79 0.3624022965515046 2202 80-81 0.49909722643933563 2202 82-83 0.6170220498261223 2202 84-85 0.6041838310967798 2202 86-87 0.5513216274112445 2202 88-89 0.4167873563980784 2202 90-91 0.3737849914720357 2202 92-93 0.31305197543980867 2202 94-95 0.37824244116549366 2202 96-97 0.5766888014389373 2202 98-99 0.49527515940641464 2202 100-101 0.4530063334234917 1102 1 0.08427414427800528 1102 2 0.03129504933128402 1102 3 0.1023413811484275 1102 4 0.09417650984462256 1102 5 0.08067241251170998 1102 6 0.16766363148386887 1102 7 0.1882005000232212 1102 8 0.0783630700932676 1102 9 0.08883043547069747 1102 10-11 0.17699975936240975 1102 12-13 0.0023447812948518276 1102 14-15 0.11678097321502179 1102 16-17 0.21165415705335278 1102 18-19 0.19367510591663262 1102 20-21 0.15579813775288898 1102 22-23 0.19315729158464734 1102 24-25 0.06484369392418898 1102 26-27 0.20730684844497915 1102 28-29 0.23633550985273644 1102 30-31 0.06391395996784155 1102 32-33 0.009606156608100491 1102 34-35 0.047408624432755175 1102 36-37 0.13403639734163164 1102 38-39 0.08661222492030873 1102 40-41 0.12144702158790466 1102 42-43 0.1696915322306083 1102 44-45 0.30228893991699834 1102 46-47 0.1259815010385168 1102 48-49 0.13778572255640853 1102 50-51 0.10560746035979918 1102 52-53 0.2449207422747719 1102 54-55 0.35433605130823764 1102 56-57 0.3214263069712828 1102 58-59 0.23979504310430144 1102 60-61 -0.06681643897441347 1102 62-63 0.2295318919935525 1102 64-65 0.2843749229744219 1102 66-67 0.34448493761711774 1102 68-69 0.3833771154759589 1102 70-71 0.39811516148292725 1102 72-73 0.08928252921307234 1102 74-75 0.14616034399850975 1102 76-77 -0.021478439533762383 1102 78-79 0.009081947755852582 1102 80-81 0.17817556251252498 1102 82-83 0.17798481160155077 1102 84-85 0.1854768051515272 1102 86-87 0.21689556780149744 1102 88-89 0.2948693376717557 1102 90-91 0.3498549650264664 1102 92-93 0.4546372802786358 1102 94-95 0.31673361406235756 1102 96-97 0.13653370173198098 1102 98-99 -0.007866503090905752 1102 100-101 0.11300061546194229 2305 1 0.1031764819230645 2305 2 0.03395823557808342 2305 3 0.08115826007636429 2305 4 0.11357530610705169 2305 5 0.04358997976472523 2305 6 0.14355363248144215 2305 7 0.045068454666918 2305 8 0.0801683439269425 2305 9 0.012477560799062815 2305 10-11 0.10712063190588594 2305 12-13 0.11761479093805605 2305 14-15 0.10508875760633174 2305 16-17 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-0.5578904065958561 1213 88-89 -0.5083666266722062 1213 90-91 -1.9064416994805846 1213 92-93 -0.9443611782008468 1213 94-95 -0.5288636692869844 1213 96-97 -0.6087934804131905 1213 98-99 -2.2509507728793388 1213 100-101 -0.8149853544672325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 11.0 10 10.0 11 16.0 12 46.0 13 146.0 14 698.0 15 2195.0 16 4380.0 17 6625.0 18 6574.0 19 6456.0 20 7225.0 21 8177.0 22 9581.0 23 11621.0 24 14404.0 25 18110.0 26 22643.0 27 29903.0 28 44089.0 29 61960.0 30 59913.0 31 57912.0 32 76856.0 33 109674.0 34 166399.0 35 268822.0 36 412449.0 37 367194.0 38 46613.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.31792094503299 23.185316162652008 21.830451258532893 12.666311633782112 2 33.39250728185603 23.778754611750603 30.139770990228058 12.688967116165308 3 38.32950053523694 15.850537900761744 29.12364494979648 16.696316614204843 4 33.28652040234588 15.327655732176575 30.88362748404204 20.502196381435496 5 33.783964883913036 14.603142520695291 35.01519192576058 16.597700669631088 6 30.1628694550843 14.821812445831927 38.31441592950489 16.70090216957888 7 35.694631030986606 19.493091202281292 26.572974896771722 18.239302869960376 8 32.704773161416185 29.31705114784135 21.049561657408436 16.92861403333403 9 34.45017965400539 24.817023136975333 21.809370128992782 18.923427080026496 10-11 27.661819314237945 25.957327846856103 27.48988174451961 18.890971094386337 12-13 25.578973615961143 24.71747471607417 26.112717921791468 23.59083374617322 14-15 24.871095093045508 26.034671959804957 25.894947169309756 23.19928577783978 16-17 26.309755141092378 24.69418725099164 26.831416328247805 22.164641279668178 18-19 23.848595186029275 24.36709842773867 28.81047430922302 22.973832077009032 20-21 25.392426740600705 25.025017547983712 29.096067384257452 20.486488327158128 22-23 23.76656073799295 29.83541693899453 25.585866485932502 20.812155837080017 24-25 23.78982074144213 24.43797021181707 28.396207658280616 23.376001388460182 26-27 22.692701027394627 24.733237693618246 26.365804334763904 26.208256944223223 28-29 24.938101478424493 27.120038094777787 25.807646636883607 22.13421378991411 30-31 22.46136422803701 25.2304854887237 28.37896175253553 23.929188530703755 32-33 22.85195103920313 27.231175324953504 25.794272821441165 24.122600814402197 34-35 23.216174682351287 26.87557463467789 28.277381170766326 21.630869512204498 36-37 22.63414401057164 27.66533907867792 28.62583795810505 21.074678952645396 38-39 22.648030805412585 25.983361518169467 27.313852384689785 24.054755291728156 40-41 25.478285799966056 25.33790185316696 24.75527551997258 24.4285368268944 42-43 23.216803381107866 27.433109702011034 27.281739765640232 22.068347151240868 44-45 22.70099428190416 25.132681683144664 27.208134624330825 24.958189410620356 46-47 25.207711119990883 24.995557825847552 25.357001127364754 24.439729926796815 48-49 22.9418722453229 27.177329458900335 27.191610045513876 22.689188250262887 50-51 25.094620325387908 24.80844550772823 26.337942798542542 23.75899136834132 52-53 22.76169860774539 24.835058363963523 24.466177901885267 27.93706512640582 54-55 25.24089440967857 24.833033620155227 27.26973334545553 22.656338624710667 56-57 22.90576886844532 24.868347548191085 27.024580434196295 25.2013031491673 58-59 22.53742412173483 25.01060235668965 27.29637157689455 25.155601944680967 60-61 24.581305546705924 25.074812967581046 27.310221143177298 23.03366034253573 62-63 22.70347316341706 24.88428967882885 27.43501121194769 24.977225945806403 64-65 25.33504240780652 25.503706855093316 26.976616312689217 22.184634424410945 66-67 23.041175772519598 29.62456899109303 24.725956350802242 22.60829888558513 68-69 22.61826307127554 28.673336715882108 25.465702521225857 23.242697691616492 70-71 22.66387772616736 29.128549498199497 25.162495892960944 23.0450768826722 72-73 22.940225505560722 29.26600123892079 25.140202705022848 22.653570550495637 74-75 23.5024413775446 28.647932315025887 25.233382726793728 22.616243580635786 76-77 23.56600007966628 27.889351777450873 25.124190116711105 23.420458026171747 78-79 23.31357111172453 27.973033288150766 25.432756946647494 23.280638653477205 80-81 23.559718840925974 28.22015431068929 25.29009118858227 22.930035659802463 82-83 23.069392252084615 27.95748060384836 25.39728744970402 23.575839694363005 84-85 22.86949899497998 28.031448791490547 25.275203015691716 23.823849197837756 86-87 22.593273824435567 28.25214038650018 25.384264203683927 23.770321585380323 88-89 22.62797322279633 28.407184904996964 25.55066599641249 23.41417587579421 90-91 23.006328262043557 27.884951877410042 25.16864469492327 23.940075165623124 92-93 22.75771716815978 27.566155691379755 24.806553992225073 24.869573148235393 94-95 22.941992182993122 28.14287299726284 24.415674889870743 24.499459929873296 96-97 22.78364001573519 28.84276016359325 24.177446152020412 24.196153668651146 98-99 22.94499285171905 27.590194162914873 24.731568588882087 24.733244396483993 100-101 23.59634790596281 27.81565484535901 24.248852883394058 24.33914436528412 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2992.0 1 1790.0 2 655.5 3 1847.5 4 2264.5 5 1277.0 6 1147.0 7 1341.5 8 1451.5 9 1456.5 10 1321.5 11 1177.5 12 1127.5 13 1107.5 14 1049.0 15 1007.0 16 1006.5 17 1091.5 18 1146.5 19 1166.5 20 1206.0 21 1274.5 22 1328.0 23 1407.5 24 1574.5 25 1762.0 26 2027.5 27 2367.0 28 2737.5 29 3265.0 30 3970.0 31 4835.0 32 6073.5 33 7866.5 34 10837.5 35 16000.5 36 22761.5 37 31448.0 38 40728.0 39 46905.5 40 54091.5 41 58575.0 42 63312.5 43 74007.0 44 79146.5 45 82529.0 46 92258.0 47 97397.5 48 94019.5 49 90875.0 50 87981.0 51 85311.0 52 82752.0 53 79971.5 54 74726.5 55 65403.0 56 56449.0 57 50088.5 58 46201.5 59 40014.5 60 30988.0 61 22978.0 62 16222.0 63 11259.5 64 8485.5 65 7002.0 66 6461.5 67 6001.5 68 5075.0 69 3908.0 70 2722.5 71 1949.5 72 1624.0 73 1333.5 74 874.0 75 520.5 76 390.0 77 321.0 78 275.5 79 209.0 80 154.5 81 127.0 82 113.0 83 84.0 84 50.5 85 39.5 86 31.5 87 25.5 88 16.0 89 17.5 90 16.0 91 9.0 92 7.5 93 6.0 94 4.5 95 3.5 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16751596344746753 2 0.005547249937112859 3 0.0018124678012348947 4 0.0 5 9.88618800673579E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.6476980011226314E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.746163335204386E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.238490005613157E-5 38-39 1.6476980011226314E-4 40-41 5.492326670408772E-5 42-43 9.336955339694912E-4 44-45 6.041559337449649E-4 46-47 0.0057394813705771664 48-49 0.003762243769230009 50-51 0.008595491239189727 52-53 0.004284014802918842 54-55 0.0203490703138645 56-57 0.011478962741154333 58-59 0.020431455213920632 60-61 0.009996034540143965 62-63 0.006563330371138483 64-65 0.013373815442445359 66-67 0.03152595508814635 68-69 0.03270680532228424 70-71 0.0371281282919633 72-73 0.03237726572205971 74-75 0.0353156604907284 76-77 0.034574196390223216 78-79 0.018097216378996903 80-81 0.0024440853683319035 82-83 0.0 84-85 0.006123944237505781 86-87 0.02691240068500298 88-89 0.02839532888601335 90-91 0.026088551684441668 92-93 0.03517835232396818 94-95 0.03229488082200358 96-97 0.03284411348904446 98-99 0.038665979759677754 100-101 0.027791172952268385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1820722.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.40409313010343 #Duplication Level Percentage of deduplicated Percentage of total 1 82.72404545615525 39.21458354902493 2 7.643364829171372 7.246535563787935 3 2.883121583161705 4.100152921008262 4 1.6552029232958807 3.1385357410054953 5 1.0061950049184099 2.3848880860098594 6 0.7537397783571049 2.143821038946223 7 0.5086258371594289 1.6877662587157671 8 0.3658350583469605 1.3873663340908913 9 0.2650280286214873 1.1307072015774629 >10 1.9401904794992295 17.625684542287175 >50 0.15828786985601215 5.152464648896459 >100 0.09136687907518481 7.816170440373527 >500 0.003728842267273615 1.1962847381487811 >1k 0.0010371349028199064 1.1484961845627701 >5k 0.0 0.0 >10k+ 2.30295211815963E-4 4.626542751564492 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 71832 3.945248093888029 TruSeq Adapter, Index 27 (97% over 39bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12927 0.7099930686837419 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3953 0.21711167328125874 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3738 0.2053031709398799 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3710 0.20376531947216545 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 2361 0.12967383268835111 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0024715470016839474 0.0 0.0 2 0.002581393535092123 0.0 0.0 3 0.0030207796687248246 0.0 0.0 4 0.003130626202133 0.0 0.0 5 0.004119245002806579 0.0 0.0 6 0.004833247469959719 0.0 0.0 7 0.00576694300392921 0.0 0.0 8 0.006755561804602789 0.0 0.0 9 0.007908950405388632 0.0 0.0 10-11 0.018618987412685736 0.0 0.0 12-13 0.034272118423350734 0.0 0.0 14-15 0.05415434097023049 0.0 0.0 16-17 0.08848138266028532 0.0 0.0 18-19 0.13008575718863175 0.0 0.0 20-21 0.14321241793090872 0.0 0.0 22-23 0.15101152180288918 0.0 0.0 24-25 0.15867331750810942 0.0 0.0 26-27 0.16833981244802887 0.0 0.0 28-29 0.17896746455526982 0.0 0.0 30-31 0.23800997626216414 0.0 0.0 32-33 0.2496262471700787 0.0 0.0 34-35 0.2594575119101104 0.0 0.0 36-37 0.2681353880493562 0.0 0.0 38-39 0.27590703028798463 0.0 0.0 40-41 0.2837885190600212 0.0 0.0 42-43 0.2927684731661396 0.0 0.0 44-45 0.3015561958387936 0.0 0.0 46-47 0.3101791487113354 0.0 0.0 48-49 0.32066949265181616 0.0 0.0 50-51 0.33162668435928166 0.0 0.0 52-53 0.34387457283429324 0.0 0.0 54-55 0.35749554297690694 0.0 0.0 56-57 0.37158336088650545 0.0 0.0 58-59 0.38968057726550237 0.0 0.0 60-61 0.40843687284494834 0.0 0.0 62-63 0.4279620941582515 0.0 0.0 64-65 0.4513319441408408 0.0 0.0 66-67 0.4761572606910885 0.0 0.0 68-69 0.5018613495086015 0.0 0.0 70-71 0.5289659816270689 0.0 0.0 72-73 0.5628536371834909 0.0 0.0 74-75 0.6015745402098728 0.0 0.0 76-77 0.6464743107404645 0.0 0.0 78-79 0.6958777891407915 0.0 0.0 80-81 0.7496202056107413 0.0 0.0 82-83 0.80899225691786 0.0 0.0 84-85 0.8767950296640563 0.0 0.0 86-87 0.950447130314238 0.0 0.0 88-89 1.0334361863041144 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 11060 0.0 71.53611 4 GATCGGA 11190 0.0 71.31093 1 ATCGGAA 11190 0.0 70.79575 2 TCGGAAG 11315 0.0 69.96591 3 GAAGAGC 11430 0.0 69.38661 6 GGAAGAG 13575 0.0 58.562702 5 GAGCACA 14850 0.0 53.566566 9 AAGAGCA 15165 0.0 52.328648 7 AGAGCAC 15490 0.0 51.261383 8 TATGCCG 9155 0.0 43.01392 48-49 GATCTCG 8925 0.0 42.944405 40-41 GTATGCC 9240 0.0 42.8478 46-47 TACTCGA 9145 0.0 42.66083 36-37 CTCGTAT 8980 0.0 42.60614 44-45 TCTCGTA 9025 0.0 42.55101 42-43 GCCGTCT 9300 0.0 42.52321 50-51 ATGCCGT 9360 0.0 42.300217 48-49 TCGTATG 9420 0.0 42.280018 44-45 CTCGATC 9010 0.0 42.193157 38-39 TGCCGTC 9345 0.0 42.089695 50-51 >>END_MODULE