##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_40255-1.341000000357f8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7572970 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.689176373338334 33.0 33.0 33.0 33.0 33.0 2 32.710727759386344 33.0 33.0 33.0 33.0 33.0 3 32.73802141035816 33.0 33.0 33.0 33.0 33.0 4 36.68012008498647 37.0 37.0 37.0 37.0 37.0 5 36.64541388649367 37.0 37.0 37.0 37.0 37.0 6 36.52952751694514 37.0 37.0 37.0 37.0 37.0 7 36.45884111517674 37.0 37.0 37.0 37.0 37.0 8 36.395266454244506 37.0 37.0 37.0 37.0 37.0 9 36.42733524627722 37.0 37.0 37.0 37.0 37.0 10-11 36.36925341048492 37.0 37.0 37.0 37.0 37.0 12-13 36.35107870492026 37.0 37.0 37.0 37.0 37.0 14-15 38.20161284146114 40.0 37.0 40.0 37.0 40.0 16-17 38.17906673339522 40.0 37.0 40.0 37.0 40.0 18-19 38.161018662427026 40.0 37.0 40.0 37.0 40.0 20-21 38.088093574911824 40.0 37.0 40.0 37.0 40.0 22-23 37.95254292569494 40.0 37.0 40.0 33.0 40.0 24-25 37.95736692473363 40.0 37.0 40.0 33.0 40.0 26-27 37.86091810742681 40.0 37.0 40.0 33.0 40.0 28-29 37.70609806984578 40.0 37.0 40.0 33.0 40.0 30-31 37.644601259479444 40.0 37.0 40.0 33.0 40.0 32-33 37.50676280244079 40.0 37.0 40.0 33.0 40.0 34-35 37.38137071716909 40.0 37.0 40.0 33.0 40.0 36-37 37.23869987600638 40.0 37.0 40.0 33.0 40.0 38-39 37.18841247225329 40.0 37.0 40.0 33.0 40.0 40-41 37.13126243732644 40.0 37.0 40.0 33.0 40.0 42-43 36.935602016117855 38.5 37.0 40.0 33.0 40.0 44-45 36.83424422650558 37.0 37.0 40.0 33.0 40.0 46-47 36.776580588593376 37.0 37.0 40.0 33.0 40.0 48-49 36.51798845103044 37.0 37.0 40.0 33.0 40.0 50-51 36.3718873836817 37.0 37.0 40.0 33.0 40.0 52-53 36.25657641585798 37.0 37.0 40.0 33.0 40.0 54-55 36.02905392468213 37.0 37.0 40.0 33.0 40.0 56-57 35.84086586900516 37.0 37.0 40.0 30.0 40.0 58-59 35.63500825963922 37.0 35.0 40.0 27.0 40.0 60-61 35.33662281773201 37.0 33.0 40.0 27.0 40.0 62-63 35.106911093005785 37.0 33.0 38.5 27.0 40.0 64-65 34.808748879237605 37.0 33.0 37.0 27.0 40.0 66-67 34.36918599968044 37.0 33.0 37.0 27.0 40.0 68-69 34.107169181972196 37.0 33.0 37.0 27.0 40.0 70-71 33.79247138176963 37.0 33.0 37.0 27.0 38.5 72-73 33.285754862359155 37.0 33.0 37.0 27.0 37.0 74-75 32.709220490771784 37.0 33.0 37.0 22.0 37.0 76-77 30.032919052894705 33.0 30.0 35.0 22.0 37.0 78-79 32.40498767326425 33.0 33.0 37.0 22.0 37.0 80-81 32.82094092542292 37.0 33.0 37.0 27.0 37.0 82-83 32.74312997410527 37.0 33.0 37.0 24.5 37.0 84-85 32.61161948350515 37.0 33.0 37.0 22.0 37.0 86-87 32.4420119187056 35.0 33.0 37.0 22.0 37.0 88-89 32.30238842884628 33.0 33.0 37.0 22.0 37.0 90-91 32.15851515323578 33.0 33.0 37.0 22.0 37.0 92-93 31.99296431915087 33.0 33.0 37.0 22.0 37.0 94-95 31.90209230988635 33.0 33.0 37.0 22.0 37.0 96-97 31.763797030755438 33.0 33.0 37.0 22.0 37.0 98-99 31.454835025095832 33.0 33.0 37.0 15.0 37.0 100-101 29.829698585891663 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12831467418368447 2306 2 0.12263083247945161 2306 3 0.12711526161718467 2306 4 0.14620978636610005 2306 5 0.1803061045253429 2306 6 0.19102243562834076 2306 7 0.23314796345631805 2306 8 0.23119972501105224 2306 9 0.24276872219910928 2306 10-11 0.23675082189182461 2306 12-13 0.24935535456562263 2306 14-15 0.47676423248455535 2306 16-17 0.47583808524047555 2306 18-19 0.5432586457296722 2306 20-21 0.5380005778465957 2306 22-23 0.5751249166274235 2306 24-25 0.6002551180048883 2306 26-27 0.6403719813901816 2306 28-29 0.5489558295870012 2306 30-31 0.5989995253752554 2306 32-33 0.61235107600681 2306 34-35 0.6436315929113974 2306 36-37 0.6719153228627377 2306 38-39 0.687650140177503 2306 40-41 0.7285299316289766 2306 42-43 0.7998492973650215 2306 44-45 0.7631055182827495 2306 46-47 0.7712168675215949 2306 48-49 0.9339391926013576 2306 50-51 0.8329662246475777 2306 52-53 0.8090715885271038 2306 54-55 0.805472996603875 2306 56-57 0.8507293840378551 2306 58-59 0.9150795609609901 2306 60-61 0.9076095150877208 2306 62-63 0.9062655076203114 2306 64-65 0.8185711456278 2306 66-67 0.8579015654249957 2306 68-69 0.881719325808227 2306 70-71 0.8097957852838675 2306 72-73 0.8071897345548678 2306 74-75 0.7115403618577361 2306 76-77 0.32256915870740954 2306 78-79 0.7521888957015719 2306 80-81 0.8787791566070169 2306 82-83 0.8587650606165127 2306 84-85 0.8618121505545133 2306 86-87 0.8539201848562072 2306 88-89 0.9079763259943618 2306 90-91 0.9925387206296037 2306 92-93 0.9593780062282775 2306 94-95 1.0539386925901084 2306 96-97 1.0184261137522803 2306 98-99 0.9763865889765775 2306 100-101 0.7854282792361857 2307 1 0.11474110389574577 2307 2 0.09450484744802168 2307 3 0.12738292966191977 2307 4 0.15487057345342947 2307 5 0.18748755350168977 2307 6 0.18671565027597126 2307 7 0.19560149602325083 2307 8 0.23159829640613339 2307 9 0.21331416585075402 2307 10-11 0.2749703669994261 2307 12-13 0.23771847003930446 2307 14-15 0.5195676742657795 2307 16-17 0.5077876203707632 2307 18-19 0.47137007509005713 2307 20-21 0.4565505269180292 2307 22-23 0.5414342366854612 2307 24-25 0.5481814721707465 2307 26-27 0.5832956784133998 2307 28-29 0.5892371024625263 2307 30-31 0.6170967507452403 2307 32-33 0.6345676865348295 2307 34-35 0.6062368691630624 2307 36-37 0.609127302928961 2307 38-39 0.6080136867856041 2307 40-41 0.6387556324503407 2307 42-43 0.6837031831756235 2307 44-45 0.6704606258579489 2307 46-47 0.5867763262981072 2307 48-49 0.6487121892590935 2307 50-51 0.5898705623026785 2307 52-53 0.6998462563191481 2307 54-55 0.6876757743599455 2307 56-57 0.6569165536859956 2307 58-59 0.644897976522472 2307 60-61 0.6918365080098212 2307 62-63 0.675933084749083 2307 64-65 0.6478021893898926 2307 66-67 0.6681135246508774 2307 68-69 0.737964978644726 2307 70-71 0.5375068848459534 2307 72-73 0.4605679201953592 2307 74-75 0.46643751480755213 2307 76-77 0.28327829016042116 2307 78-79 0.5395966446589 2307 80-81 0.6069274147458188 2307 82-83 0.6299844285812668 2307 84-85 0.5743396011824586 2307 86-87 0.7011058279498457 2307 88-89 0.6720886514891262 2307 90-91 0.7789529717712682 2307 92-93 0.7554828500429949 2307 94-95 0.8195008866605136 2307 96-97 0.7858250251053072 2307 98-99 0.7133566583488395 2307 100-101 0.5654111706241771 2201 1 0.10665545214757799 2201 2 0.08708042948839534 2201 3 0.10508793377197634 2201 4 0.13573561749217333 2201 5 0.18652505276040898 2201 6 0.18864235423249198 2201 7 0.22607361616797306 2201 8 0.23682262146742517 2201 9 0.1776348517888593 2201 10-11 0.26070359983004465 2201 12-13 0.2364670098132109 2201 14-15 0.4171375664963435 2201 16-17 0.4385881725681884 2201 18-19 0.45266364600930586 2201 20-21 0.46679771762320144 2201 22-23 0.4846528289494785 2201 24-25 0.4870792564860196 2201 26-27 0.5397378469339174 2201 28-29 0.5517482536668936 2201 30-31 0.5711840431108115 2201 32-33 0.6061768230599043 2201 34-35 0.6154940180639841 2201 36-37 0.5986706901938348 2201 38-39 0.6184113513602156 2201 40-41 0.6108440863287683 2201 42-43 0.6394616310641723 2201 44-45 0.6718877069124503 2201 46-47 0.7054316492389745 2201 48-49 0.7177920914689935 2201 50-51 0.6923632714579924 2201 52-53 0.7176746521942903 2201 54-55 0.7525567551154637 2201 56-57 0.7390396408501019 2201 58-59 0.669413743275598 2201 60-61 0.7422967718422058 2201 62-63 0.7548949437995631 2201 64-65 0.7039944879368676 2201 66-67 0.6944475158485162 2201 68-69 0.8267066460987849 2201 70-71 0.8124330364915551 2201 72-73 0.7885629159150511 2201 74-75 0.7874184065236847 2201 76-77 0.4618898155401929 2201 78-79 0.7791763691021458 2201 80-81 0.7612680813438217 2201 82-83 0.7751354777450317 2201 84-85 0.7279554926985483 2201 86-87 0.836936949485775 2201 88-89 0.8808758537370736 2201 90-91 0.8723879874893541 2201 92-93 0.8050675811783776 2201 94-95 0.9044770186850997 2201 96-97 0.8543251285306006 2201 98-99 0.8228002848964273 2201 100-101 0.7283497030859571 1101 1 0.10637611899200294 1101 2 0.07537248612534597 1101 3 0.10911021237371443 1101 4 0.12151993038983733 1101 5 0.17254238304972347 1101 6 0.14543740844695208 1101 7 0.09533090708544734 1101 8 0.18044457800416325 1101 9 0.18245788335169522 1101 10-11 0.1667480540818005 1101 12-13 0.1553743149256377 1101 14-15 0.37312952225186535 1101 16-17 0.3891697059195707 1101 18-19 0.36902611724332957 1101 20-21 0.3689909269753926 1101 22-23 0.4087936746559393 1101 24-25 0.36631534285213263 1101 26-27 0.4218831324488619 1101 28-29 0.3578831940978162 1101 30-31 0.42902864193279555 1101 32-33 0.44674839398741284 1101 34-35 0.4927221706429279 1101 36-37 0.4528637241653328 1101 38-39 0.5185134399353473 1101 40-41 0.473208178020343 1101 42-43 0.5007303596298343 1101 44-45 0.5495191600259162 1101 46-47 0.5303544807965181 1101 48-49 0.5558800303749649 1101 50-51 0.45948460818910064 1101 52-53 0.4488948764901721 1101 54-55 0.5061430994894636 1101 56-57 0.5925578125476747 1101 58-59 0.6084562403814076 1101 60-61 0.43759460221917834 1101 62-63 0.5079841051790055 1101 64-65 0.3527883047292164 1101 66-67 0.3555832015222862 1101 68-69 0.530955177676411 1101 70-71 0.5151535075025464 1101 72-73 0.44264525177070624 1101 74-75 0.38304344635805165 1101 76-77 0.19648539886144079 1101 78-79 0.6022871472151081 1101 80-81 0.5191133337778737 1101 82-83 0.4573360638442807 1101 84-85 0.41609625534311334 1101 86-87 0.5019152955370245 1101 88-89 0.6229694150828138 1101 90-91 0.49318670831112854 1101 92-93 0.577484285826479 1101 94-95 0.606886805052774 1101 96-97 0.629777673574015 1101 98-99 0.5865576304483646 1101 100-101 0.5779556156669372 2304 1 0.06361679305052093 2304 2 0.10077418509002456 2304 3 0.10054190415672792 2304 4 0.10762523750663178 2304 5 0.1601800280765957 2304 6 0.16939524044724408 2304 7 0.08630211621525063 2304 8 0.14582638629802602 2304 9 0.11802028414509635 2304 10-11 0.14123981185379364 2304 12-13 0.17876655289302334 2304 14-15 0.34375719608601685 2304 16-17 0.3109296521585989 2304 18-19 0.35169666918024944 2304 20-21 0.37514733148502444 2304 22-23 0.35465017878573946 2304 24-25 0.3554371005045809 2304 26-27 0.3982739710627925 2304 28-29 0.4183781642937845 2304 30-31 0.34684102402830064 2304 32-33 0.3882835567397507 2304 34-35 0.385740403387139 2304 36-37 0.4382212543857307 2304 38-39 0.470597470095548 2304 40-41 0.4459072174905572 2304 42-43 0.514691655954401 2304 44-45 0.5208386282771613 2304 46-47 0.43083699777545803 2304 48-49 0.4991352051050768 2304 50-51 0.49850432675584955 2304 52-53 0.5158598893173547 2304 54-55 0.5231536545570634 2304 56-57 0.638096369003236 2304 58-59 0.6000214031810174 2304 60-61 0.5786021506091998 2304 62-63 0.5244425648447972 2304 64-65 0.4404178589185008 2304 66-67 0.43147935909733093 2304 68-69 0.6301289530197067 2304 70-71 0.49417021216278556 2304 72-73 0.3965688075353455 2304 74-75 0.4762822586898281 2304 76-77 0.2517054626928683 2304 78-79 0.4755170123594752 2304 80-81 0.5024797255506854 2304 82-83 0.5260888042332184 2304 84-85 0.5175189081869576 2304 86-87 0.5219691176792836 2304 88-89 0.617826061288838 2304 90-91 0.6124452789687425 2304 92-93 0.6295728062625088 2304 94-95 0.6979397705671815 2304 96-97 0.5810883197538992 2304 98-99 0.6304961473116428 2304 100-101 0.6868902219587198 2202 1 0.08065096625990265 2202 2 0.06678417424721061 2202 3 0.0700166344512283 2202 4 0.07700417999630815 2202 5 0.13722069850147278 2202 6 0.11659506772004136 2202 7 0.13833643527540573 2202 8 0.1341767991305005 2202 9 0.11467140087582095 2202 10-11 0.13934337786772488 2202 12-13 0.14982388870058116 2202 14-15 0.1579727844667218 2202 16-17 0.18339095745069045 2202 18-19 0.2012816676432081 2202 20-21 0.17054930493320342 2202 22-23 0.2548388747776329 2202 24-25 0.23599327312108187 2202 26-27 0.13946317591182122 2202 28-29 0.16456937739572197 2202 30-31 0.18146077361873125 2202 32-33 0.2732156638296246 2202 34-35 0.21635788319054683 2202 36-37 0.23780428323998848 2202 38-39 0.30308941900837993 2202 40-41 0.2725585785524913 2202 42-43 0.3086738407191518 2202 44-45 0.2726506308730521 2202 46-47 0.2861334874590753 2202 48-49 0.3568701451404692 2202 50-51 0.30337586713196174 2202 52-53 0.27647862318806915 2202 54-55 0.32322558267183155 2202 56-57 0.39962169630108946 2202 58-59 0.3904399550000477 2202 60-61 0.4131337290461232 2202 62-63 0.4365582511823689 2202 64-65 0.3576958023678003 2202 66-67 0.364892374321407 2202 68-69 0.3179048260113575 2202 70-71 0.3139113008677228 2202 72-73 0.2790701557200279 2202 74-75 0.2681145407756915 2202 76-77 0.17314932797263083 2202 78-79 0.42311976173375143 2202 80-81 0.42682039904217106 2202 82-83 0.5041716843695596 2202 84-85 0.5095358597861974 2202 86-87 0.45309983013660826 2202 88-89 0.5556403042522859 2202 90-91 0.5891664115485966 2202 92-93 0.5853192518044565 2202 94-95 0.6119778573142369 2202 96-97 0.5243629412960864 2202 98-99 0.48774319782263476 2202 100-101 0.418680840587875 1102 1 0.06661147975965065 1102 2 0.05276857195523377 1102 3 0.06102778582706492 1102 4 0.06577240741547286 1102 5 0.10195210917392927 1102 6 0.07787997371891464 1102 7 0.07728343420450301 1102 8 0.11123572634758006 1102 9 0.13187031191694132 1102 10-11 0.11606354292460708 1102 12-13 0.07908558399005727 1102 14-15 0.1606566046465403 1102 16-17 0.1754501514642186 1102 18-19 0.13362595770055208 1102 20-21 0.18227512057332973 1102 22-23 0.18017868466738207 1102 24-25 0.18011896327518429 1102 26-27 0.2390316097370615 1102 28-29 0.27632960373929194 1102 30-31 0.2345931964806809 1102 32-33 0.16874509688963002 1102 34-35 0.15715462236098432 1102 36-37 0.2801249198802722 1102 38-39 0.27114957939307516 1102 40-41 0.2568490424603027 1102 42-43 0.16305637377720217 1102 44-45 0.19839816074721028 1102 46-47 0.2393474319544069 1102 48-49 0.2885104995946506 1102 50-51 0.290604837835744 1102 52-53 0.24327748268581217 1102 54-55 0.2061028372080287 1102 56-57 0.2921598559083449 1102 58-59 0.21648775499386375 1102 60-61 0.11296390964159997 1102 62-63 0.2008116780183471 1102 64-65 0.24849690018261583 1102 66-67 0.18099020994250026 1102 68-69 0.28365342888581324 1102 70-71 0.16719020683143526 1102 72-73 0.07239498019234247 1102 74-75 0.04419382632834612 1102 76-77 -0.0934417210222449 1102 78-79 0.25390725762613897 1102 80-81 0.1868683714137731 1102 82-83 0.2718918212931669 1102 84-85 0.20037459759255682 1102 86-87 0.1845797846886228 1102 88-89 0.21943321761089152 1102 90-91 0.2491625296938409 1102 92-93 0.24086304490923993 1102 94-95 0.2810240776852382 1102 96-97 0.2197301294659404 1102 98-99 0.12835295360929067 1102 100-101 0.09867157449905406 2305 1 -0.009999853464783826 2305 2 -0.01918561170466404 2305 3 0.037098459816775176 2305 4 0.03692332337629978 2305 5 0.04792153340800098 2305 6 0.036650028260901024 2305 7 0.04165068497118796 2305 8 0.04792959111046713 2305 9 -0.020696696235937395 2305 10-11 -0.00480032169249256 2305 12-13 0.01847468160298149 2305 14-15 -0.05870525023922113 2305 16-17 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-0.2825189754385491 1213 70-71 -0.10340477016600857 1213 72-73 -0.02811562037801707 1213 74-75 -0.02474468951567843 1213 76-77 -2.3602635946667228 1213 78-79 -2.1645192373356537 1213 80-81 -1.5589665779497857 1213 82-83 -1.1947716396151193 1213 84-85 -0.8772361321178863 1213 86-87 -0.695486345527172 1213 88-89 -0.7179019666456448 1213 90-91 -1.962893578751892 1213 92-93 -1.111610683498533 1213 94-95 -0.7499892396415859 1213 96-97 -0.6917663575365331 1213 98-99 -2.5047390876128013 1213 100-101 -1.0598178275917327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 25.0 10 51.0 11 45.0 12 109.0 13 277.0 14 960.0 15 2642.0 16 5855.0 17 10092.0 18 12462.0 19 14630.0 20 18014.0 21 22562.0 22 28207.0 23 35319.0 24 44293.0 25 55323.0 26 70520.0 27 94401.0 28 135021.0 29 186294.0 30 183506.0 31 185491.0 32 246050.0 33 351350.0 34 540283.0 35 923889.0 36 1665186.0 37 2217510.0 38 522315.0 39 280.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.30714688298147 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21.40477815303062 27.292562176414847 30.766286084927337 20.536373585627192 20-21 22.778982354347104 27.76188866455301 30.419571185413385 19.039557795686502 22-23 21.270360241754556 30.667083059882717 28.712255561556432 19.3503011368063 24-25 21.35270574160468 26.898152244099737 30.653303789662445 21.095838224633138 26-27 21.113433699063908 27.114058288887975 29.090574767891592 22.681933244156518 28-29 22.412897449745607 29.326004196504147 28.482444800388752 19.778653553361494 30-31 20.19749186910816 28.730636725089365 29.942552261530153 21.129319144272326 32-33 20.06280891116695 30.198482233522643 28.640738045971396 21.097970809339003 34-35 22.095716739931625 29.936537448319484 28.758815893896315 19.208929917852576 36-37 21.721294401093257 30.53059305203857 29.008699955809934 18.739412591058237 38-39 20.359165044336073 28.59720640019818 31.427245091841982 19.61638346362377 40-41 20.597212466211225 28.01572540958685 28.06691406861499 23.320148055586937 42-43 20.8528272327497 29.671979571317763 29.799744667882056 19.67544852805048 44-45 20.271228163814687 28.110057371506354 29.99749238173952 21.62122208293944 46-47 22.04601800342764 28.018471433299023 28.577833276417298 21.35767728685604 48-49 20.86439129131469 29.70396834934046 29.42027712814635 20.011363231198505 50-51 22.136827703876065 28.282321122713235 28.86614098360223 20.714710189808475 52-53 20.530250393965208 28.130644256830124 27.762089451345968 23.577015897858704 54-55 22.45664568493432 27.925358848641363 29.47203245007896 20.14596301634536 56-57 20.94502899973561 27.554971924706074 29.456865418987345 22.04313365657097 58-59 20.675280497385888 27.785755422088776 29.75570050104302 21.78326357948231 60-61 21.88655882976152 27.92596432627818 29.541411533413005 20.646065310547296 62-63 20.176103627189327 28.095722301529342 29.595379615531403 22.132794455749927 64-65 22.076828513510648 28.191180309204427 29.53498496542315 20.197006211861776 66-67 21.083233055991084 30.81305881777967 27.850665793120026 20.25304233310922 68-69 20.94564146959886 30.545743654413492 28.011022645309392 20.497592230678258 70-71 20.677874754260987 31.277261632747283 27.881738813405455 20.163124799586278 72-73 20.77617441892028 31.487637916303175 27.674857553879484 20.06133011089706 74-75 21.58913949949825 30.745651212221002 27.37470581312084 20.29050347515991 76-77 21.671606484895094 29.74847347332141 27.649200431044164 20.930719610739327 78-79 21.33223952370547 29.650917960778035 28.212601883473614 20.804240632042877 80-81 20.881668097886738 30.1379623079622 28.09697161932196 20.8833979748291 82-83 20.151505948128673 30.16124453153782 28.29117241980359 21.39607710052991 84-85 20.421509962809694 29.90097440917262 28.431275513657077 21.24624011436061 86-87 20.2503408530688 30.101508008602472 28.853805815618717 20.79434532271001 88-89 20.27469560671418 30.46686801492334 28.743804288015184 20.514632090347305 90-91 20.819717136196665 29.57576373154256 28.50596534889021 21.098553783370566 92-93 20.442548780116493 30.0092023188549 28.000880273492413 21.547368627536194 94-95 20.2681853086473 30.39132113755228 27.644790419177497 21.695703134622928 96-97 20.51202339180823 30.770554731855377 27.350727265726427 21.36669461060997 98-99 20.679515813856565 29.940697005854343 27.658973272536507 21.720813907752586 100-101 20.93894391923681 30.13717917932356 27.24133645155828 21.682540449881348 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3054.0 1 1907.5 2 933.0 3 1425.5 4 1474.0 5 1083.5 6 1032.0 7 1178.0 8 1278.0 9 1243.0 10 1170.0 11 1176.0 12 1175.0 13 1183.5 14 1242.0 15 1271.5 16 1362.5 17 1506.5 18 1705.0 19 2104.5 20 2601.0 21 3255.5 22 4294.5 23 6012.5 24 8229.5 25 10952.5 26 14854.5 27 19872.5 28 25990.5 29 32966.0 30 41095.5 31 51126.0 32 66388.0 33 89149.5 34 120648.5 35 170816.5 36 241560.0 37 328401.0 38 410846.5 39 451770.5 40 478368.0 41 474467.0 42 448919.5 43 458364.5 44 454956.0 45 441049.0 46 450736.0 47 418778.0 48 333486.0 49 262857.5 50 218848.0 51 193036.0 52 173479.5 53 148372.5 54 116468.5 55 85261.5 56 64136.5 57 53060.0 58 45428.5 59 35284.5 60 25399.5 61 18788.0 62 13504.5 63 9336.0 64 6730.5 65 4695.5 66 3372.0 67 2554.5 68 1782.0 69 1181.0 70 759.0 71 492.0 72 339.0 73 239.0 74 177.5 75 124.5 76 84.0 77 60.0 78 42.5 79 38.5 80 28.5 81 18.0 82 15.5 83 13.0 84 6.5 85 4.5 86 5.0 87 3.5 88 2.0 89 3.0 90 2.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17003896753849546 2 0.006602429429933038 3 0.0017034267929227239 4 0.0 5 0.0012016421562478128 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.5749474776738847E-4 12-13 0.0 14-15 6.6024294299330386E-6 16-17 0.0 18-19 2.6409717719732154E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.6024294299330386E-6 38-39 2.244826006177233E-4 40-41 4.6217006009531267E-5 42-43 0.0011620275796682146 44-45 6.206283664137056E-4 46-47 0.005440401850264824 48-49 0.003961457657959823 50-51 0.007922915315919645 52-53 0.0037435774867720325 54-55 0.01931870851198407 56-57 0.010986442571408576 58-59 0.01933851580027387 60-61 0.009263208490196052 62-63 0.005810137898341073 64-65 0.012207892015946189 66-67 0.030371175377691975 68-69 0.031341732503892133 70-71 0.03555408248018941 72-73 0.03037777780712191 74-75 0.033368678338881574 76-77 0.03322342489142305 78-79 0.016750363463740117 80-81 0.0023372600181962955 82-83 0.0 84-85 0.005711101456892078 86-87 0.02499019539229655 88-89 0.026515356590611085 90-91 0.024699688497379496 92-93 0.0342732111707824 94-95 0.03098520131467575 96-97 0.03243773578926102 98-99 0.038921321489455256 100-101 0.02635689828429269 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7572970.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.69920688671465 #Duplication Level Percentage of deduplicated Percentage of total 1 87.85474447690052 35.756184214448254 2 6.282986091445024 5.114251016041433 3 1.7185896997694226 2.0983571323287764 4 0.763316272263965 1.2426546753946768 5 0.5952289437910243 1.2112672964155773 6 0.4084851978882505 0.9975014147408687 7 0.3239768696236601 0.9229921150326466 8 0.1913024413534784 0.6228686110863051 9 0.14374903339622375 0.5265424484962348 >10 1.318581860690188 10.953726550532284 >50 0.18343542420431816 5.08979402263459 >100 0.17848615090995026 15.114016678714673 >500 0.022035640870590505 6.370051139913094 >1k 0.014690806712899289 10.070861130060196 >5k 2.6081362798903375E-4 0.6869092554286481 >10k+ 1.30276552399376E-4 3.222022298731697 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 206041 2.7207423243456663 TruSeq Adapter, Index 7 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15056 0.19881235499414365 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11955 0.15786408766969895 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10021 0.13232589063471795 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 9609 0.12688548878445313 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9548 0.1260799923940013 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00245610374793509 0.0 0.0 2 0.0027069960662725458 0.0 0.0 3 0.0030107078200494655 0.0 0.0 4 0.003169166126367858 0.0 0.0 5 0.0036973604807625012 0.0 0.0 6 0.00410671110541835 0.0 0.0 7 0.004899002637010315 0.0 0.0 8 0.005598860156583217 0.0 0.0 9 0.006034620498958797 0.0 1.3204858859866077E-5 10-11 0.011197720313166432 0.0 1.9807288289799116E-5 12-13 0.014954502658798333 0.0 2.6409717719732154E-5 14-15 0.02036189236191349 0.0 2.6409717719732154E-5 16-17 0.02855550728446039 0.0 2.6409717719732154E-5 18-19 0.03879587533028653 0.0 3.961457657959823E-5 20-21 0.044665435093497005 0.0 5.281943543946431E-5 22-23 0.050336921973809484 0.0 5.281943543946431E-5 24-25 0.056840314962293526 0.0 6.602429429933038E-5 26-27 0.06337672009792723 0.0 6.602429429933038E-5 28-29 0.07024324670505759 0.0 6.602429429933038E-5 30-31 0.12065279540259634 0.0 8.58315825891295E-5 32-33 0.13109783876075043 0.0 9.243401201906253E-5 34-35 0.1399054796202811 0.0 9.243401201906253E-5 36-37 0.14971008732373164 0.0 9.243401201906253E-5 38-39 0.1605116618711021 0.0 9.243401201906253E-5 40-41 0.17228379354467271 0.0 9.243401201906253E-5 42-43 0.18505949449159312 0.0 9.243401201906253E-5 44-45 0.19828416063974902 0.0 9.243401201906253E-5 46-47 0.21339051917543578 0.0 1.0563887087892862E-4 48-49 0.2280016955038776 0.0 1.0563887087892862E-4 50-51 0.24405220144804485 0.0 1.0563887087892862E-4 52-53 0.26228150910409 0.0 1.0563887087892862E-4 54-55 0.28196995366415023 0.0 1.0563887087892862E-4 56-57 0.30277420879786926 0.0 1.0563887087892862E-4 58-59 0.3256186146254376 0.0 1.0563887087892862E-4 60-61 0.34989574763930137 0.0 1.0563887087892862E-4 62-63 0.3777910119807685 0.0 1.0563887087892862E-4 64-65 0.4082414165116196 0.0 1.1884372973879469E-4 66-67 0.44006512636389683 0.0 1.1884372973879469E-4 68-69 0.47532209951973925 0.0 1.1884372973879469E-4 70-71 0.5143820720272232 0.0 1.1884372973879469E-4 72-73 0.5566970422436639 0.0 1.3204858859866076E-4 74-75 0.6054823933014392 0.0 1.3204858859866076E-4 76-77 0.6570011501432067 0.0 1.3204858859866076E-4 78-79 0.7143102375950254 0.0 1.3204858859866076E-4 80-81 0.7810145821256389 0.0 1.3204858859866076E-4 82-83 0.8547835261462808 0.0 1.5845830631839293E-4 84-85 0.9374129304618928 0.0 1.5845830631839293E-4 86-87 1.0282293472706217 6.6024294299330386E-6 1.6506073574832595E-4 88-89 1.1245323829356249 1.3204858859866077E-5 1.8486802403812507E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 34415 0.0 65.326004 4 TCGGAAG 34570 0.0 65.15803 3 GATCGGA 35360 0.0 63.84719 1 GAAGAGC 35645 0.0 63.031834 6 ATCGGAA 36080 0.0 62.42161 2 GGAAGAG 40400 0.0 55.883476 5 GCCGTCT 25815 0.0 43.270195 50-51 TATGCCG 25680 0.0 43.153744 48-49 ATGCCGT 25770 0.0 43.14128 48-49 TGCCGTC 25855 0.0 43.047085 50-51 TCGTATG 26110 0.0 42.784336 44-45 CGTATGC 25915 0.0 42.759174 46-47 TCTCGTA 25780 0.0 42.5021 42-43 CTCGTAT 25890 0.0 42.340706 44-45 CATCTCG 25910 0.0 42.00334 40-41 CGTCTTC 26565 0.0 41.97773 52-53 ATCTCGT 25945 0.0 41.83823 42-43 GTATGCC 26635 0.0 41.799465 46-47 GTCACGA 27170 0.0 40.664833 28-29 TGTGTTT 86665 0.0 40.64812 1 >>END_MODULE