FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_40255-1.341000000357f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_40255-1.341000000357f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7572970
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2060412.7207423243456663TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150560.19881235499414365No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA119550.15786408766969895No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC100210.13232589063471795No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG96090.12688548878445313No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC95480.1260799923940013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA344150.065.3260044
TCGGAAG345700.065.158033
GATCGGA353600.063.847191
GAAGAGC356450.063.0318346
ATCGGAA360800.062.421612
GGAAGAG404000.055.8834765
GCCGTCT258150.043.27019550-51
TATGCCG256800.043.15374448-49
ATGCCGT257700.043.1412848-49
TGCCGTC258550.043.04708550-51
TCGTATG261100.042.78433644-45
CGTATGC259150.042.75917446-47
TCTCGTA257800.042.502142-43
CTCGTAT258900.042.34070644-45
CATCTCG259100.042.0033440-41
CGTCTTC265650.041.9777352-53
ATCTCGT259450.041.8382342-43
GTATGCC266350.041.79946546-47
GTCACGA271700.040.66483328-29
TGTGTTT866650.040.648121
Produced by FastQC (version 0.11.2)