##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_35011-1.341000000358a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5977721 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70200700233417 33.0 33.0 33.0 33.0 33.0 2 32.72768083354844 33.0 33.0 33.0 33.0 33.0 3 32.760310492911934 33.0 33.0 33.0 33.0 33.0 4 36.701578578190585 37.0 37.0 37.0 37.0 37.0 5 36.667176002359426 37.0 37.0 37.0 37.0 37.0 6 36.55026639751169 37.0 37.0 37.0 37.0 37.0 7 36.485993240567765 37.0 37.0 37.0 37.0 37.0 8 36.429288687110024 37.0 37.0 37.0 37.0 37.0 9 36.4679930695996 37.0 37.0 37.0 37.0 37.0 10-11 36.404413738948335 37.0 37.0 37.0 37.0 37.0 12-13 36.38424100087642 37.0 37.0 37.0 37.0 37.0 14-15 38.2479557844871 40.0 37.0 40.0 37.0 40.0 16-17 38.25384314523879 40.0 37.0 40.0 37.0 40.0 18-19 38.24086261302594 40.0 37.0 40.0 37.0 40.0 20-21 38.19794542100576 40.0 37.0 40.0 37.0 40.0 22-23 38.06747487880415 40.0 37.0 40.0 37.0 40.0 24-25 38.0732565972885 40.0 37.0 40.0 37.0 40.0 26-27 38.00544747739147 40.0 37.0 40.0 37.0 40.0 28-29 37.86046003150699 40.0 37.0 40.0 33.0 40.0 30-31 37.821347383057855 40.0 37.0 40.0 33.0 40.0 32-33 37.69484432277786 40.0 37.0 40.0 33.0 40.0 34-35 37.56477602417376 40.0 37.0 40.0 33.0 40.0 36-37 37.36988235148479 40.0 37.0 40.0 33.0 40.0 38-39 37.425047856867195 40.0 37.0 40.0 33.0 40.0 40-41 37.265739903217295 40.0 37.0 40.0 33.0 40.0 42-43 37.128799504025025 40.0 37.0 40.0 33.0 40.0 44-45 37.10099710240742 40.0 37.0 40.0 33.0 40.0 46-47 37.035472381531356 38.5 37.0 40.0 33.0 40.0 48-49 36.7870067873693 37.0 37.0 40.0 33.0 40.0 50-51 36.675105194772385 37.0 37.0 40.0 33.0 40.0 52-53 36.5779677572774 37.0 37.0 40.0 33.0 40.0 54-55 36.357516769350724 37.0 37.0 40.0 33.0 40.0 56-57 36.20724888297731 37.0 37.0 40.0 33.0 40.0 58-59 35.97800499554931 37.0 37.0 40.0 33.0 40.0 60-61 35.72463176183699 37.0 37.0 40.0 27.0 40.0 62-63 35.51162550075522 37.0 33.0 40.0 27.0 40.0 64-65 35.22167578580533 37.0 33.0 38.5 27.0 40.0 66-67 34.77232334864742 37.0 33.0 37.0 27.0 40.0 68-69 34.53706981306087 37.0 33.0 37.0 27.0 40.0 70-71 34.2053527590197 37.0 33.0 37.0 27.0 40.0 72-73 33.657530269478954 37.0 33.0 37.0 27.0 38.5 74-75 33.03340202729435 37.0 33.0 37.0 22.0 37.0 76-77 30.390524833795354 33.0 30.0 35.0 22.0 37.0 78-79 32.667866566539324 35.0 33.0 37.0 24.5 37.0 80-81 32.9931111706284 37.0 33.0 37.0 27.0 37.0 82-83 32.90873971869881 37.0 33.0 37.0 27.0 37.0 84-85 32.766610134531206 37.0 33.0 37.0 24.5 37.0 86-87 32.59133154591859 37.0 33.0 37.0 22.0 37.0 88-89 32.43705326829405 37.0 33.0 37.0 22.0 37.0 90-91 32.28731593528704 33.0 33.0 37.0 22.0 37.0 92-93 32.12669159366923 33.0 33.0 37.0 22.0 37.0 94-95 32.0310469491634 33.0 33.0 37.0 22.0 37.0 96-97 31.897846938657725 33.0 33.0 37.0 22.0 37.0 98-99 31.610435063797723 33.0 33.0 37.0 15.0 37.0 100-101 30.035421442385818 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13187453762756007 2306 2 0.14128242046125905 2306 3 0.13849274278462076 2306 4 0.16436316791077132 2306 5 0.15650805859783645 2306 6 0.20639183193656407 2306 7 0.2762701308645106 2306 8 0.23323679388228413 2306 9 0.2463044554826368 2306 10-11 0.2572934428994742 2306 12-13 0.24581183047549615 2306 14-15 0.5492767607366176 2306 16-17 0.5662839720463495 2306 18-19 0.5792743614547007 2306 20-21 0.6184811447331455 2306 22-23 0.5934104536468894 2306 24-25 0.5702500363195284 2306 26-27 0.5983421251711007 2306 28-29 0.6024279414134526 2306 30-31 0.6766073639029528 2306 32-33 0.6547556275323245 2306 34-35 0.6406570928016677 2306 36-37 0.6817938384011768 2306 38-39 0.7275142916865249 2306 40-41 0.7386738635203116 2306 42-43 0.7357025165641389 2306 44-45 0.7749596610268341 2306 46-47 0.7338109410313294 2306 48-49 0.7611101907352733 2306 50-51 0.8250037927460241 2306 52-53 0.7991553752691303 2306 54-55 0.747844822847405 2306 56-57 0.6866425936202987 2306 58-59 0.7435715753022833 2306 60-61 0.7429541369797832 2306 62-63 0.7709057508958779 2306 64-65 0.7962052998497171 2306 66-67 0.7105610307755086 2306 68-69 0.7534333607794679 2306 70-71 0.7085480905625658 2306 72-73 0.5672942327956534 2306 74-75 0.5574548957839411 2306 76-77 0.2616829457556449 2306 78-79 0.5725188184123269 2306 80-81 0.6725280152095152 2306 82-83 0.6743922614358056 2306 84-85 0.7144840514920006 2306 86-87 0.7091590000599268 2306 88-89 0.6509990800432419 2306 90-91 0.770381702651413 2306 92-93 0.602549075785646 2306 94-95 0.777285599502278 2306 96-97 0.8752260424389355 2306 98-99 0.7691909631670448 2306 100-101 0.6145210147222002 2307 1 0.11570704190681624 2307 2 0.07172315566290877 2307 3 0.10600704443805142 2307 4 0.16607830329119366 2307 5 0.19568332751719453 2307 6 0.21641522528025803 2307 7 0.19451556586265895 2307 8 0.2346549447517461 2307 9 0.19867908029331005 2307 10-11 0.27669775973875943 2307 12-13 0.22568454127525683 2307 14-15 0.44626565670500185 2307 16-17 0.4680859659839669 2307 18-19 0.4479478550002156 2307 20-21 0.4443023866333462 2307 22-23 0.4462273329088404 2307 24-25 0.4821209900162913 2307 26-27 0.5094228918916528 2307 28-29 0.5468263949679368 2307 30-31 0.5858113811887335 2307 32-33 0.5532648250828345 2307 34-35 0.5697613977040064 2307 36-37 0.5318995989926876 2307 38-39 0.49316710284092125 2307 40-41 0.5682750139864865 2307 42-43 0.5318805075093209 2307 44-45 0.5875960419173367 2307 46-47 0.6241263780027353 2307 48-49 0.6241059871088694 2307 50-51 0.6342550263612168 2307 52-53 0.5874001037940602 2307 54-55 0.4938605021236313 2307 56-57 0.593267762182812 2307 58-59 0.6089735330968153 2307 60-61 0.5873518596998935 2307 62-63 0.519746492564316 2307 64-65 0.5774769826534296 2307 66-67 0.5344316646768732 2307 68-69 0.6960119260275874 2307 70-71 0.4317630110112489 2307 72-73 0.29909723218418804 2307 74-75 0.26648875046793563 2307 76-77 0.05422398376633808 2307 78-79 0.29846024934614945 2307 80-81 0.46437381934546096 2307 82-83 0.5011156962155852 2307 84-85 0.4217922512099861 2307 86-87 0.4705316686706169 2307 88-89 0.4528936906270076 2307 90-91 0.42528193600904274 2307 92-93 0.5186516711999474 2307 94-95 0.5026703782598929 2307 96-97 0.5158863557188376 2307 98-99 0.39881717239340375 2307 100-101 0.2175834432582242 2201 1 0.08323239174936248 2201 2 0.07653898325592223 2201 3 0.08755406055684745 2201 4 0.10572047563773168 2201 5 0.1737765479020723 2201 6 0.14762806234067938 2201 7 0.21472976303485325 2201 8 0.2309629103709412 2201 9 0.16452917772055997 2201 10-11 0.1986845725723967 2201 12-13 0.20989250436387152 2201 14-15 0.42191650017024784 2201 16-17 0.3643665875766118 2201 18-19 0.37174447412844813 2201 20-21 0.3163548664864422 2201 22-23 0.3959689973378673 2201 24-25 0.3680779122939768 2201 26-27 0.42332401600980063 2201 28-29 0.3450744473337579 2201 30-31 0.4305888099699473 2201 32-33 0.45470403464452147 2201 34-35 0.4650192030352116 2201 36-37 0.5414718545243389 2201 38-39 0.5484292377250526 2201 40-41 0.5340704135140228 2201 42-43 0.6328978539807864 2201 44-45 0.6262562318800349 2201 46-47 0.6291449964588409 2201 48-49 0.6261211936173154 2201 50-51 0.5892618928923312 2201 52-53 0.5587515003333507 2201 54-55 0.5443457773604052 2201 56-57 0.5237260291623258 2201 58-59 0.5512623502170726 2201 60-61 0.5788887013200821 2201 62-63 0.5739363073722927 2201 64-65 0.42797977008006427 2201 66-67 0.53470788660875 2201 68-69 0.5746469362478663 2201 70-71 0.5353666179414134 2201 72-73 0.5098853860862604 2201 74-75 0.6089511681860031 2201 76-77 0.3389659874109583 2201 78-79 0.6136572902833208 2201 80-81 0.6648508529725135 2201 82-83 0.5910241011116 2201 84-85 0.5405448624069678 2201 86-87 0.6696666344334687 2201 88-89 0.6727532770321147 2201 90-91 0.7208085093909276 2201 92-93 0.703456353418936 2201 94-95 0.7082132940547581 2201 96-97 0.7129136061041557 2201 98-99 0.6981522655122738 2201 100-101 0.6411543289299679 1101 1 0.09154952473199529 1101 2 0.08530029107278381 1101 3 0.09978591144216153 1101 4 0.14143494560857306 1101 5 0.1496617304051071 1101 6 0.08966476777074206 1101 7 0.12005407129527867 1101 8 0.1266579774021821 1101 9 0.1858384576762404 1101 10-11 0.14969071698359215 1101 12-13 0.16090316857258813 1101 14-15 0.33947695863557925 1101 16-17 0.32337821941886347 1101 18-19 0.35938609096012186 1101 20-21 0.3848005195810984 1101 22-23 0.3605248112592605 1101 24-25 0.31736562968466586 1101 26-27 0.3385582559498488 1101 28-29 0.3508266944337848 1101 30-31 0.32202300875117373 1101 32-33 0.3354331701457127 1101 34-35 0.3742162502007744 1101 36-37 0.36741711946759636 1101 38-39 0.3825634277356684 1101 40-41 0.3630980260374699 1101 42-43 0.4664248477770201 1101 44-45 0.5031570547786259 1101 46-47 0.34916858583333266 1101 48-49 0.39675260315872407 1101 50-51 0.35549827336438966 1101 52-53 0.3082144050762494 1101 54-55 0.4799289615440969 1101 56-57 0.43168202811761347 1101 58-59 0.45312583848568977 1101 60-61 0.25292798087405544 1101 62-63 0.3694333058519135 1101 64-65 0.25763406906673225 1101 66-67 0.3735715486580631 1101 68-69 0.4810114152781537 1101 70-71 0.4283792843542855 1101 72-73 0.37103442797516806 1101 74-75 0.30436834042692595 1101 76-77 0.20796380101953815 1101 78-79 0.49219377278815557 1101 80-81 0.39603091213732 1101 82-83 0.3726300641047331 1101 84-85 0.26918490559948083 1101 86-87 0.3164207782169086 1101 88-89 0.4524496365931583 1101 90-91 0.4040537786952356 1101 92-93 0.5472004928400054 1101 94-95 0.4988397807649889 1101 96-97 0.5089819731348264 1101 98-99 0.5497732655410665 1101 100-101 0.4091563848425501 2304 1 0.07424036579093496 2304 2 0.06785416620666496 2304 3 0.06435084707538863 2304 4 0.09556109819806835 2304 5 0.1336020549366097 2304 6 0.10909385308200115 2304 7 0.0996428224898196 2304 8 0.11635932596755794 2304 9 0.10329396739304997 2304 10-11 0.13146529287008235 2304 12-13 0.1392707270912581 2304 14-15 0.3076613330435194 2304 16-17 0.26629075773966804 2304 18-19 0.2809982039412162 2304 20-21 0.25917301288026096 2304 22-23 0.2713403387551878 2304 24-25 0.2620706264003658 2304 26-27 0.22968250736575158 2304 28-29 0.2800846356524005 2304 30-31 0.2571255240193864 2304 32-33 0.2396564210772283 2304 34-35 0.2302066580979627 2304 36-37 0.2484509333010081 2304 38-39 0.3235662571359157 2304 40-41 0.2623387307075191 2304 42-43 0.2938655340780869 2304 44-45 0.25149617263151924 2304 46-47 0.26798772397224724 2304 48-49 0.34840236934691404 2304 50-51 0.2764931380638558 2304 52-53 0.34394033169201776 2304 54-55 0.32480321015496116 2304 56-57 0.35467013171739126 2304 58-59 0.27706259340788364 2304 60-61 0.30404886945191123 2304 62-63 0.3023844583051698 2304 64-65 0.3344846157603385 2304 66-67 0.22069677895156303 2304 68-69 0.2689989025713331 2304 70-71 0.3155731987715029 2304 72-73 0.20699102476383047 2304 74-75 0.24749814886056498 2304 76-77 0.08452550509043277 2304 78-79 0.34249320242004444 2304 80-81 0.2491874317313787 2304 82-83 0.28977525664083004 2304 84-85 0.26159239309142635 2304 86-87 0.4066061879237495 2304 88-89 0.5201877817876266 2304 90-91 0.4374558127153563 2304 92-93 0.4251026236739577 2304 94-95 0.48630072664253277 2304 96-97 0.3723444577431856 2304 98-99 0.4481289811956337 2304 100-101 0.4181291355959793 2202 1 0.037371659584245265 2202 2 0.04162029554770896 2202 3 0.065290847841176 2202 4 0.10001700814178349 2202 5 0.10728873411109419 2202 6 0.15135464107449792 2202 7 0.1485353063126169 2202 8 0.12991754475226003 2202 9 0.10513472245680333 2202 10-11 0.14610087505793246 2202 12-13 0.16256206989480404 2202 14-15 0.13355490810956638 2202 16-17 0.14284434352670417 2202 18-19 0.09441140224117817 2202 20-21 0.19961649796660197 2202 22-23 0.22613654429181196 2202 24-25 0.28827865182442025 2202 26-27 0.2969744053632226 2202 28-29 0.21966039090759182 2202 30-31 0.30053403188783534 2202 32-33 0.31199005434291394 2202 34-35 0.3227455216734114 2202 36-37 0.29300285350623767 2202 38-39 0.3681416294987443 2202 40-41 0.32357231419720023 2202 42-43 0.4449366754802426 2202 44-45 0.3108018930435037 2202 46-47 0.36726932216499364 2202 48-49 0.43514807138007683 2202 50-51 0.3927641492825984 2202 52-53 0.3353266892369007 2202 54-55 0.35702408161799326 2202 56-57 0.5069354186952211 2202 58-59 0.48856850909498917 2202 60-61 0.5215800751799904 2202 62-63 0.4747334072656173 2202 64-65 0.48257015391556024 2202 66-67 0.5061631418569519 2202 68-69 0.36129319364611234 2202 70-71 0.4861943459170419 2202 72-73 0.3889792891127257 2202 74-75 0.3077271184972048 2202 76-77 0.12519489667445782 2202 78-79 0.40627780631788823 2202 80-81 0.40693286573988274 2202 82-83 0.6001468536849046 2202 84-85 0.4923463081658994 2202 86-87 0.5338595095150538 2202 88-89 0.5102756555862982 2202 90-91 0.550765630075702 2202 92-93 0.46886722144552806 2202 94-95 0.5739194232769051 2202 96-97 0.5112027684220557 2202 98-99 0.5184553012170774 2202 100-101 0.5168694197021217 1102 1 0.07272731301737423 1102 2 0.08942825038579372 1102 3 0.09345885391448405 1102 4 0.11800385152317006 1102 5 0.10328188491055812 1102 6 0.1214104580456663 1102 7 0.09429585852941358 1102 8 0.13772255657448795 1102 9 0.11043549533290076 1102 10-11 0.10759151258874056 1102 12-13 0.07897807856332406 1102 14-15 0.13850335516705314 1102 16-17 0.17479947591225198 1102 18-19 0.15548215836215462 1102 20-21 0.17239176191834105 1102 22-23 0.20431090460390067 1102 24-25 0.22703248526978115 1102 26-27 0.21806195005191853 1102 28-29 0.2565266810020077 1102 30-31 0.1900542199732982 1102 32-33 0.1639869574875945 1102 34-35 0.1301726235051035 1102 36-37 0.19199370005805605 1102 38-39 0.19131202677244374 1102 40-41 0.22182556740023784 1102 42-43 0.08924277588390339 1102 44-45 0.1993503842462303 1102 46-47 0.19818018557227646 1102 48-49 0.21352894445248438 1102 50-51 0.20779894710075553 1102 52-53 0.3028894707882088 1102 54-55 0.20997997316785444 1102 56-57 0.24498811205638305 1102 58-59 0.22401312187101752 1102 60-61 0.09776723092237916 1102 62-63 0.28362950877291127 1102 64-65 0.23394384000948065 1102 66-67 0.2475850867222249 1102 68-69 0.16314495259973683 1102 70-71 0.1684230450036992 1102 72-73 -0.022235106248011505 1102 74-75 0.061246865130293315 1102 76-77 0.048024506089433316 1102 78-79 0.17554435994600226 1102 80-81 0.07535296787590795 1102 82-83 0.14252845582260676 1102 84-85 0.07410746905187438 1102 86-87 0.07812815335327628 1102 88-89 0.1707770384693177 1102 90-91 0.17977557278722145 1102 92-93 0.1725525127204861 1102 94-95 0.17681745781052882 1102 96-97 0.17759302570184232 1102 98-99 0.07167088047283343 1102 100-101 0.10334392081076871 2305 1 0.06372115869971395 2305 2 0.03256541389151835 2305 3 0.034512061450044484 2305 4 0.07247661559812002 2305 5 0.04710960788613505 2305 6 0.010917012831157535 2305 7 0.04244074349354321 2305 8 0.07893881498941369 2305 9 0.042907967549204784 2305 10-11 0.010652996967145612 2305 12-13 0.011346694193719031 2305 14-15 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30 143237.0 31 135198.0 32 177939.0 33 252277.0 34 385910.0 35 653984.0 36 1224176.0 37 1878648.0 38 558768.0 39 536.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.23068249975239 25.227767213486736 22.078584360048914 15.462965926711956 2 28.88034067350405 25.851917978176232 31.58766714804053 13.680074200279188 3 35.85527383370533 19.413072725100985 28.281338716536354 16.450314724657332 4 27.99013202523169 19.82113250183473 31.98784285850745 20.200892614426134 5 34.7951765063882 18.47438938888953 30.131076396294937 16.599357708427327 6 29.017781191193098 19.438963444429742 34.753629351386586 16.78962601299057 7 31.071908508275982 23.20916282308927 27.520839463735424 18.198089204899325 8 27.720765154479444 29.286662927225944 25.35889513746125 17.633676780833365 9 32.16377947381619 23.52483496636929 25.77830246677622 18.5330830930383 10-11 24.52735670894762 30.070532014679195 29.145821870612348 16.256289405760835 12-13 22.676928213946418 28.522157524581694 29.101190905363435 19.699723356108457 14-15 21.8904913762285 28.807241087364233 29.64004174835192 19.66222578805535 16-17 23.206210192814286 27.24036468078721 30.306742318686336 19.24668280771217 18-19 20.687312276864112 27.39956265930511 32.40851297890441 19.504612084926368 20-21 22.82443426181985 27.678792636859434 31.909619067199692 17.58715403412103 22-23 20.849132972248118 31.656169633878868 29.66182262437474 17.832874769498275 24-25 20.68671321394893 27.35895502650592 31.779293479906475 20.17503827963868 26-27 20.6601479058658 27.62817133820732 29.467676728304987 22.244004027621898 28-29 22.340445464082382 29.626290688374386 29.42302760533655 18.61023624220669 30-31 19.954745295071483 28.01983398020751 31.365415013514347 20.66000571120666 32-33 19.999319138514494 30.062468623075585 29.668932357331496 20.269279881078425 34-35 22.313127360745007 29.880300535940034 29.746118964066742 18.06045313924822 36-37 20.213785523577553 29.682554552571883 31.899628955521504 18.204030968329064 38-39 20.83328732909425 27.45553899402949 31.17273169825903 20.538441978617232 40-41 22.640462464104193 27.672641968055366 30.759639380780424 18.927256187060014 42-43 20.388686449481952 29.824683495810895 31.16444165479187 18.622188399915284 44-45 20.142366233463594 27.74610858741805 31.478450298832417 20.633074880285942 46-47 22.309061440487277 27.65656739375052 29.33915682988272 20.695214335879484 48-49 20.361095431886156 30.047095112687995 30.773981936863677 18.817827518562176 50-51 22.761907056317167 27.862376268433632 30.28476788839825 19.09094878685095 52-53 20.58077050285296 27.68848976816792 28.354414984392236 23.376324744586885 54-55 22.663553825678896 27.9374885175923 30.174562714777498 19.224394941951306 56-57 20.498160124235532 28.149513621639095 30.28347337492141 21.068852879203966 58-59 20.514501658610026 28.14369784660541 30.602871343380006 20.738929151404562 60-61 22.283688760797276 28.377768284208578 30.48211725814605 18.856425696848103 62-63 20.152806820278748 28.237993719960564 30.42637951159301 21.182819948167676 64-65 22.16996864701833 28.018794221494648 30.37905578327786 19.432181348209166 66-67 20.356749312447235 31.408640197593503 28.53329328361144 19.701317206347827 68-69 20.19554564900263 31.812794597581846 28.49902754297825 19.49263221043727 70-71 19.910080747762958 32.49681809316195 28.254362105391046 19.338739053684044 72-73 19.930992492057435 32.307806484794725 28.077586675014683 19.683614348133155 74-75 20.174649141229597 31.608013795884833 28.309677786433205 19.907659276452364 76-77 20.109604991455857 31.272531237389096 28.465351898759174 20.152511872395873 78-79 19.909162026343864 31.166296101744017 28.672112661775493 20.252429210136622 80-81 19.82474476448823 31.169735849908847 28.462673152582553 20.54284623302037 82-83 19.531214320641595 30.96622441897171 28.542482996446306 20.96007826394039 84-85 19.67912420191558 30.683531750098012 28.82230857956594 20.81503546842046 86-87 20.017066105683355 30.52060239889307 29.074509723865088 20.387821771558485 88-89 20.252311687194684 30.63387237212627 28.684248198302555 20.429567742376488 90-91 20.317598457420992 30.437882573206114 28.55262908001045 20.691889889362443 92-93 19.88715762162987 30.908103269577413 28.68623916556449 20.518499943228225 94-95 19.58217384163296 31.117704677913643 28.67116917176001 20.62895230869339 96-97 19.77581840564592 31.23784416111 28.5726943953179 20.413643037926178 98-99 20.30432153346296 30.629454259098214 28.139667189951854 20.926557017486974 100-101 20.482080023774607 30.6102751549332 27.60964297260231 21.29800184868989 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 145.0 1 82.5 2 37.0 3 75.5 4 101.0 5 80.0 6 59.0 7 53.0 8 49.0 9 58.0 10 60.0 11 67.0 12 75.5 13 81.0 14 98.0 15 127.5 16 151.5 17 216.0 18 331.0 19 505.5 20 769.0 21 1219.5 22 2020.0 23 3368.5 24 5237.0 25 8000.5 26 12700.0 27 19018.5 28 26847.5 29 36138.5 30 46527.5 31 58338.0 32 76112.5 33 104779.0 34 142220.0 35 189955.5 36 258463.5 37 330651.5 38 390662.0 39 422860.5 40 430155.0 41 419293.5 42 390510.5 43 362917.5 44 328437.0 45 295494.5 46 275611.5 47 247751.5 48 211354.5 49 182482.5 50 153253.0 51 125167.0 52 101486.5 53 79569.0 54 63687.0 55 50338.0 56 36551.5 57 25047.5 58 17558.0 59 12832.0 60 9258.5 61 6527.0 62 4436.5 63 3016.5 64 2072.5 65 1437.5 66 974.5 67 671.0 68 470.0 69 319.0 70 222.5 71 160.5 72 110.5 73 77.0 74 57.5 75 38.5 76 29.0 77 20.5 78 15.5 79 13.5 80 9.0 81 6.5 82 3.5 83 2.0 84 1.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17655558029556748 2 0.0064405816196507 3 0.0019238100941813777 4 0.0 5 0.0010371845725151776 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.6728783427664154E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.509317514149623E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.6728783427664155E-5 38-39 1.840166177043057E-4 40-41 5.018635028299246E-5 42-43 0.0012546587570748116 44-45 6.60786945392734E-4 46-47 0.005470312180846179 48-49 0.00406509437292239 50-51 0.00781234186071916 52-53 0.004148738290060711 54-55 0.019857065928637355 56-57 0.011492674214805274 58-59 0.019748328836357534 60-61 0.009627414862620722 62-63 0.006189649868235738 64-65 0.013006629115008881 66-67 0.030379470704638104 68-69 0.03142501966886712 70-71 0.03575777457663213 72-73 0.030939884949464858 74-75 0.03389251522444758 76-77 0.03328191462933784 78-79 0.017322655239346233 80-81 0.002567868256146448 82-83 0.0 84-85 0.006047455209100592 86-87 0.02589615674602411 88-89 0.027108993544529763 90-91 0.025017895616071744 92-93 0.034135082584148706 94-95 0.03115735913402449 96-97 0.03246220424138229 98-99 0.038986429778171315 100-101 0.026715867133979655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5977721.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25146438397076 #Duplication Level Percentage of deduplicated Percentage of total 1 90.97841376112353 50.26690587632873 2 4.547149528450883 5.024733404395869 3 1.5065641236886917 2.4971962206646166 4 0.6064972304295301 1.3403944050421637 5 0.3538798095221142 0.9776188846008724 6 0.2710963259081619 0.8987081397324083 7 0.19133018057529608 0.7399890860344266 8 0.14949493071948067 0.660785107218525 9 0.1765455972015555 0.8778962498335733 >10 0.9820365029223845 10.787709751638106 >50 0.13807109844915605 5.3644685424821485 >100 0.0832912151941969 9.182758120501788 >500 0.012357723819932364 4.814626361889425 >1k 0.0031507918477277517 2.4253685475435907 >5k 0.0 0.0 >10k+ 1.2118014740333821E-4 4.140841302093764 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 215155 3.5992813983790812 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11875 0.19865430320351185 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10203 0.17068377731245737 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10152 0.16983060935764652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.862528211002153E-4 0.0 0.0 2 8.531679548108719E-4 0.0 0.0 3 9.368118719491927E-4 0.0 0.0 4 0.0010204557890875134 0.0 0.0 5 0.0012212011902194833 0.0 1.6728783427664155E-5 6 0.0013215738907854683 0.0 1.6728783427664155E-5 7 0.001505590508489774 0.0 1.6728783427664155E-5 8 0.0016728783427664155 0.0 1.6728783427664155E-5 9 0.0018067086101877287 0.0 3.345756685532831E-5 10-11 0.003044638583834876 0.0 5.018635028299246E-5 12-13 0.004316026124337352 0.0 5.018635028299246E-5 14-15 0.005629235623408988 0.0 5.018635028299246E-5 16-17 0.006691513371065662 0.0 5.018635028299246E-5 18-19 0.00817201070441394 0.0 5.018635028299246E-5 20-21 0.010338388158296448 0.0 5.855074199682454E-5 22-23 0.01263859587960027 0.0 6.691513371065662E-5 24-25 0.01513118461032223 0.0 6.691513371065662E-5 26-27 0.01758195138247503 0.0 6.691513371065662E-5 28-29 0.020266921122615123 0.0 6.691513371065662E-5 30-31 0.029651768625534716 0.0 6.691513371065662E-5 32-33 0.03341574489675915 0.0 6.691513371065662E-5 34-35 0.03708771285913143 0.0 8.364391713832077E-5 36-37 0.040977155006063345 0.0 8.364391713832077E-5 38-39 0.04490841911156442 0.0 8.364391713832077E-5 40-41 0.04919098766904645 0.0 8.364391713832077E-5 42-43 0.05395869094593073 0.0 8.364391713832077E-5 44-45 0.058810038139953336 0.0 8.364391713832077E-5 46-47 0.06412979126995054 0.0 8.364391713832077E-5 48-49 0.06964192540936587 0.0 8.364391713832077E-5 50-51 0.07532971177477168 0.0 8.364391713832077E-5 52-53 0.0812266079330233 0.0 8.364391713832077E-5 54-55 0.087717375902957 0.0 9.200830885215284E-5 56-57 0.0949943966939909 0.0 1.0037270056598492E-4 58-59 0.1029991195641282 0.0 1.0037270056598492E-4 60-61 0.11122968101053897 0.0 1.08737092279817E-4 62-63 0.1196860810332232 0.0 1.4219465913514534E-4 64-65 0.1294473261632652 0.0 1.505590508489774E-4 66-67 0.1392922152104456 0.0 1.505590508489774E-4 68-69 0.1503248478809901 0.0 1.505590508489774E-4 70-71 0.16322273990371916 0.0 1.505590508489774E-4 72-73 0.1776011292597965 8.364391713832078E-6 1.756522259904736E-4 74-75 0.19287450852925386 1.6728783427664155E-5 1.840166177043057E-4 76-77 0.21027244329402459 1.6728783427664155E-5 1.840166177043057E-4 78-79 0.23017969557294493 1.6728783427664155E-5 1.9238100941813778E-4 80-81 0.2529475698179958 1.6728783427664155E-5 2.0074540113196985E-4 82-83 0.2789775568314413 1.6728783427664155E-5 2.17474184559634E-4 84-85 0.3083198429635642 1.6728783427664155E-5 2.17474184559634E-4 86-87 0.3419697908283107 1.6728783427664155E-5 2.17474184559634E-4 88-89 0.38134098262531824 1.6728783427664155E-5 2.17474184559634E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 27560 0.0 83.586945 3 CGGAAGA 27785 0.0 82.756256 4 GATCGGA 27930 0.0 82.67131 1 ATCGGAA 28260 0.0 81.50447 2 GAAGAGC 29725 0.0 77.49765 6 GGAAGAG 33600 0.0 68.77319 5 AGAGCAC 34300 0.0 67.43776 8 GAGCACA 34385 0.0 67.35392 9 AAGAGCA 35985 0.0 64.04245 7 TATGCCG 24895 0.0 45.56565 48-49 CGTATGC 24950 0.0 45.52822 46-47 GCCGTCT 25135 0.0 45.387035 50-51 ATGCCGT 25075 0.0 45.33328 48-49 TGCCGTC 25120 0.0 45.300674 50-51 TCGTATG 25225 0.0 45.26422 44-45 TCTCGTA 25140 0.0 45.104034 42-43 CTCGTAT 25115 0.0 45.0748 44-45 TCGTATC 24880 0.0 45.04515 38-39 CGTATCT 24990 0.0 44.989395 38-39 AATTCGT 24795 0.0 44.95941 34-35 >>END_MODULE