FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_35011-1.341000000358a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_35011-1.341000000358a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5977721
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2151553.5992813983790812TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC118750.19865430320351185No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA102030.17068377731245737No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG101520.16983060935764652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG275600.083.5869453
CGGAAGA277850.082.7562564
GATCGGA279300.082.671311
ATCGGAA282600.081.504472
GAAGAGC297250.077.497656
GGAAGAG336000.068.773195
AGAGCAC343000.067.437768
GAGCACA343850.067.353929
AAGAGCA359850.064.042457
TATGCCG248950.045.5656548-49
CGTATGC249500.045.5282246-47
GCCGTCT251350.045.38703550-51
ATGCCGT250750.045.3332848-49
TGCCGTC251200.045.30067450-51
TCGTATG252250.045.2642244-45
TCTCGTA251400.045.10403442-43
CTCGTAT251150.045.074844-45
TCGTATC248800.045.0451538-39
CGTATCT249900.044.98939538-39
AATTCGT247950.044.9594134-35
Produced by FastQC (version 0.11.2)