##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_35010-1.341000000357eb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6796058 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70613081877759 33.0 33.0 33.0 33.0 33.0 2 32.73181659132397 33.0 33.0 33.0 33.0 33.0 3 32.75939184156462 33.0 33.0 33.0 33.0 33.0 4 36.70513435877092 37.0 37.0 37.0 37.0 37.0 5 36.67001753075091 37.0 37.0 37.0 37.0 37.0 6 36.55800862794285 37.0 37.0 37.0 37.0 37.0 7 36.511990480363764 37.0 37.0 37.0 37.0 37.0 8 36.45431940104101 37.0 37.0 37.0 37.0 37.0 9 36.47142770117618 37.0 37.0 37.0 37.0 37.0 10-11 36.42015437478609 37.0 37.0 37.0 37.0 37.0 12-13 36.405599025199606 37.0 37.0 37.0 37.0 37.0 14-15 38.29548990017449 40.0 37.0 40.0 37.0 40.0 16-17 38.28354010516097 40.0 37.0 40.0 37.0 40.0 18-19 38.28670517526484 40.0 37.0 40.0 37.0 40.0 20-21 38.211983770591715 40.0 37.0 40.0 37.0 40.0 22-23 38.11161955945638 40.0 37.0 40.0 37.0 40.0 24-25 38.098069498524 40.0 37.0 40.0 37.0 40.0 26-27 38.025596382491145 40.0 37.0 40.0 37.0 40.0 28-29 37.90261052804435 40.0 37.0 40.0 33.0 40.0 30-31 37.83986334430931 40.0 37.0 40.0 33.0 40.0 32-33 37.723356760639774 40.0 37.0 40.0 33.0 40.0 34-35 37.637062397054294 40.0 37.0 40.0 33.0 40.0 36-37 37.54307563296252 40.0 37.0 40.0 33.0 40.0 38-39 37.48192849443015 40.0 37.0 40.0 33.0 40.0 40-41 37.41933419343978 40.0 37.0 40.0 33.0 40.0 42-43 37.2984672879484 40.0 37.0 40.0 33.0 40.0 44-45 37.20306382023226 40.0 37.0 40.0 33.0 40.0 46-47 37.120696880456286 40.0 37.0 40.0 33.0 40.0 48-49 36.92608737300358 37.0 37.0 40.0 33.0 40.0 50-51 36.792244195090746 37.0 37.0 40.0 33.0 40.0 52-53 36.686603837106745 37.0 37.0 40.0 33.0 40.0 54-55 36.517726011167056 37.0 37.0 40.0 33.0 40.0 56-57 36.3408584064468 37.0 37.0 40.0 33.0 40.0 58-59 36.153887150462815 37.0 37.0 40.0 33.0 40.0 60-61 35.91721347875489 37.0 37.0 40.0 33.0 40.0 62-63 35.70981699979606 37.0 35.0 40.0 27.0 40.0 64-65 35.453694479947046 37.0 33.0 40.0 27.0 40.0 66-67 35.133061916187295 37.0 33.0 38.5 27.0 40.0 68-69 34.87566446607725 37.0 33.0 37.0 27.0 40.0 70-71 34.59801189159951 37.0 33.0 37.0 27.0 40.0 72-73 34.22478185147919 37.0 33.0 37.0 27.0 40.0 74-75 33.79133646299075 37.0 33.0 37.0 27.0 38.5 76-77 31.099120769716798 33.0 30.0 35.0 24.5 37.0 78-79 33.47140496740904 37.0 33.0 37.0 27.0 37.0 80-81 33.823760185684115 37.0 33.0 37.0 27.0 37.0 82-83 33.738898123000126 37.0 33.0 37.0 27.0 37.0 84-85 33.60384100606558 37.0 33.0 37.0 27.0 37.0 86-87 33.427244293677305 37.0 33.0 37.0 27.0 37.0 88-89 33.27052528097906 37.0 33.0 37.0 27.0 37.0 90-91 33.10963811668471 37.0 33.0 37.0 27.0 37.0 92-93 32.95320934577074 35.0 33.0 37.0 27.0 37.0 94-95 32.863137939670324 33.0 33.0 37.0 27.0 37.0 96-97 32.73669154971897 33.0 33.0 37.0 27.0 37.0 98-99 32.446674895947034 33.0 33.0 37.0 27.0 37.0 100-101 30.819778171404657 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10686076884673668 2306 2 0.1014823757487946 2306 3 0.10435134523987699 2306 4 0.14567138358182774 2306 5 0.13959217415220593 2306 6 0.1805930151280677 2306 7 0.20212230552898802 2306 8 0.18569644762077786 2306 9 0.22373337155821815 2306 10-11 0.22132688893032793 2306 12-13 0.24187952663845635 2306 14-15 0.48502510103113394 2306 16-17 0.4960928983539148 2306 18-19 0.507371721672996 2306 20-21 0.5242716195845318 2306 22-23 0.47068650465060813 2306 24-25 0.48041146532055734 2306 26-27 0.5001528346284161 2306 28-29 0.44459050672043077 2306 30-31 0.4770116839328864 2306 32-33 0.5557133511431189 2306 34-35 0.6259196764030435 2306 36-37 0.5640343701617283 2306 38-39 0.6493300749584776 2306 40-41 0.6280387268438332 2306 42-43 0.7020382726451331 2306 44-45 0.641288666312704 2306 46-47 0.5957504018087008 2306 48-49 0.7219990454330798 2306 50-51 0.6872592979989705 2306 52-53 0.6652701227645537 2306 54-55 0.6320565802616258 2306 56-57 0.5888237332785522 2306 58-59 0.6372186064226284 2306 60-61 0.6341067865489762 2306 62-63 0.5929650663544308 2306 64-65 0.5063035775180396 2306 66-67 0.5999202194215201 2306 68-69 0.6394539560968511 2306 70-71 0.5474857099172823 2306 72-73 0.44389757710685274 2306 74-75 0.456387260887972 2306 76-77 0.0963279984505867 2306 78-79 0.4900914770856417 2306 80-81 0.5135004647033838 2306 82-83 0.6237642108394681 2306 84-85 0.611296420787319 2306 86-87 0.5637791711443114 2306 88-89 0.6333734523884118 2306 90-91 0.7861547776556108 2306 92-93 0.6901632379595029 2306 94-95 0.6769330521594767 2306 96-97 0.7455720980505447 2306 98-99 0.5541681978758746 2306 100-101 0.481973362672548 2307 1 0.06792105164157647 2307 2 0.07296501055304816 2307 3 0.10515339073852914 2307 4 0.13391841275798555 2307 5 0.17441630510732864 2307 6 0.17693822296379125 2307 7 0.19695114282261272 2307 8 0.24849534355730896 2307 9 0.18436264125716662 2307 10-11 0.1975406710071752 2307 12-13 0.19135489969639252 2307 14-15 0.46208408225539443 2307 16-17 0.4264400305963676 2307 18-19 0.4312765558656366 2307 20-21 0.46495830326352205 2307 22-23 0.42773697866557114 2307 24-25 0.48585821994634415 2307 26-27 0.46220164739799685 2307 28-29 0.42210714168002994 2307 30-31 0.49938683773154224 2307 32-33 0.5102776882350284 2307 34-35 0.5359411522128212 2307 36-37 0.5063921913196054 2307 38-39 0.5252542015799193 2307 40-41 0.5603988237089936 2307 42-43 0.4904761356827265 2307 44-45 0.5054097335210201 2307 46-47 0.5155364308921122 2307 48-49 0.529953244259552 2307 50-51 0.4426079951827191 2307 52-53 0.47989746156670776 2307 54-55 0.46317727502437833 2307 56-57 0.45011301566032813 2307 58-59 0.5112213096362765 2307 60-61 0.5396898480849757 2307 62-63 0.4285324188108319 2307 64-65 0.44770806679552066 2307 66-67 0.4623056672888808 2307 68-69 0.5086747692089446 2307 70-71 0.439495470335757 2307 72-73 0.4095423528094173 2307 74-75 0.36069000155553255 2307 76-77 0.07275508883007831 2307 78-79 0.3583333906309605 2307 80-81 0.4465046212712096 2307 82-83 0.4921481729023611 2307 84-85 0.4745769993738591 2307 86-87 0.5113504974001373 2307 88-89 0.5391062039613601 2307 90-91 0.5769765592099034 2307 92-93 0.5528103555260557 2307 94-95 0.5645180588285328 2307 96-97 0.4953168399001271 2307 98-99 0.4545589778488903 2307 100-101 0.28734530368336664 2201 1 0.10122733566579711 2201 2 0.08950085790640117 2201 3 0.08621779454962564 2201 4 0.09679418084228075 2201 5 0.15767448347403956 2201 6 0.1592969173190113 2201 7 0.16241886640999326 2201 8 0.17601601792195964 2201 9 0.14347701664218704 2201 10-11 0.22390929316072317 2201 12-13 0.20800520720005977 2201 14-15 0.3769303871092049 2201 16-17 0.40787588998963287 2201 18-19 0.3728048985204566 2201 20-21 0.35239983815254305 2201 22-23 0.37855096850345404 2201 24-25 0.36068517995494176 2201 26-27 0.4372759250167704 2201 28-29 0.360397260907547 2201 30-31 0.4147868840555162 2201 32-33 0.4702436984295417 2201 34-35 0.4603878696630801 2201 36-37 0.5066254308712033 2201 38-39 0.4992047990673072 2201 40-41 0.49575745381535796 2201 42-43 0.5179902366873321 2201 44-45 0.5546873927893472 2201 46-47 0.5471563173714031 2201 48-49 0.6031723091093255 2201 50-51 0.5321533562262104 2201 52-53 0.6202846581190755 2201 54-55 0.5253630157732161 2201 56-57 0.515827651928106 2201 58-59 0.5447887733667187 2201 60-61 0.5725374816483679 2201 62-63 0.5887218962516485 2201 64-65 0.5812337282753077 2201 66-67 0.6678717780139607 2201 68-69 0.6014007861630901 2201 70-71 0.534664968541378 2201 72-73 0.595172690796197 2201 74-75 0.4995641779958575 2201 76-77 0.29820318412139457 2201 78-79 0.5048682310589356 2201 80-81 0.5944930246223024 2201 82-83 0.5497301995273034 2201 84-85 0.4865944378096785 2201 86-87 0.524594940548802 2201 88-89 0.5896271326219207 2201 90-91 0.6630746180845364 2201 92-93 0.6010008458312228 2201 94-95 0.6687963173929319 2201 96-97 0.753871251394088 2201 98-99 0.6746070646096811 2201 100-101 0.49967542889305605 1101 1 0.09034082809270672 1101 2 0.07584367735324093 1101 3 0.10823030438261583 1101 4 0.1403079562247953 1101 5 0.17688263891005107 1101 6 0.1332055482732315 1101 7 0.10994666625900607 1101 8 0.15533039741436738 1101 9 0.1475101880035652 1101 10-11 0.1716342877994279 1101 12-13 0.1745948662601151 1101 14-15 0.3541945391292174 1101 16-17 0.3447333773369152 1101 18-19 0.3100120782256255 1101 20-21 0.31258499560009056 1101 22-23 0.3685701740436187 1101 24-25 0.38794761224141894 1101 26-27 0.3782915417821684 1101 28-29 0.3859946226302782 1101 30-31 0.36910519436739975 1101 32-33 0.4489605695859993 1101 34-35 0.4577346457650293 1101 36-37 0.41921424876758806 1101 38-39 0.46825823396159905 1101 40-41 0.4894324997845487 1101 42-43 0.5556360752412886 1101 44-45 0.5577250999677048 1101 46-47 0.46472188161754957 1101 48-49 0.568555004500773 1101 50-51 0.4622828022326786 1101 52-53 0.45755935123382585 1101 54-55 0.43345165955648923 1101 56-57 0.523103194607053 1101 58-59 0.5438257622195195 1101 60-61 0.3674900677234092 1101 62-63 0.5071885572756898 1101 64-65 0.375501848520976 1101 66-67 0.40673549301559575 1101 68-69 0.5177246354434217 1101 70-71 0.44239133896691385 1101 72-73 0.4118399042905807 1101 74-75 0.3526388634074564 1101 76-77 0.197234776022448 1101 78-79 0.5310436055210062 1101 80-81 0.4887194472738301 1101 82-83 0.44609469871157614 1101 84-85 0.44122333041065787 1101 86-87 0.37214817184884197 1101 88-89 0.6069292764876835 1101 90-91 0.47280227093991556 1101 92-93 0.49635573463737614 1101 94-95 0.6359802553354328 1101 96-97 0.5243473140507149 1101 98-99 0.5105878960001959 1101 100-101 0.3628072665178763 2304 1 0.07139258917005975 2304 2 0.0958827854215798 2304 3 0.07296639904848234 2304 4 0.08148206803338098 2304 5 0.11436394828059804 2304 6 0.10402543400165598 2304 7 0.08569064155786066 2304 8 0.1255254972290345 2304 9 0.1688251300869723 2304 10-11 0.14874788559770735 2304 12-13 0.14390862965136364 2304 14-15 0.27470013283396355 2304 16-17 0.24004191991821955 2304 18-19 0.31009390047128704 2304 20-21 0.2682827325054902 2304 22-23 0.33365035984436275 2304 24-25 0.2893833279128586 2304 26-27 0.2519748319429098 2304 28-29 0.32834368494310695 2304 30-31 0.34469396154971577 2304 32-33 0.34221564913077174 2304 34-35 0.3071293305806577 2304 36-37 0.29224783217530614 2304 38-39 0.3546135591001942 2304 40-41 0.3098934195315337 2304 42-43 0.37123068929020064 2304 44-45 0.3113889575216007 2304 46-47 0.2528461768802188 2304 48-49 0.2975341353805021 2304 50-51 0.26658658175681893 2304 52-53 0.33938600215823556 2304 54-55 0.280299759346029 2304 56-57 0.3551863678022329 2304 58-59 0.43155053336432303 2304 60-61 0.43601040948200875 2304 62-63 0.2716872262346044 2304 64-65 0.27574146980329317 2304 66-67 0.29329233808535093 2304 68-69 0.33322877908008053 2304 70-71 0.26873823620240245 2304 72-73 0.32210045625398465 2304 74-75 0.3145328528398963 2304 76-77 0.04522210409336935 2304 78-79 0.31671986955821296 2304 80-81 0.35599250455674536 2304 82-83 0.34579029736661226 2304 84-85 0.3073083766928235 2304 86-87 0.4139027203608876 2304 88-89 0.47000041905945267 2304 90-91 0.4433925037217392 2304 92-93 0.39680531222562365 2304 94-95 0.43799791849210123 2304 96-97 0.27030480799417944 2304 98-99 0.28839358715743657 2304 100-101 0.3379552595925652 2202 1 0.09884640746023621 2202 2 0.07139617166657075 2202 3 0.06319979309228785 2202 4 0.12481208569879243 2202 5 0.11356390416102613 2202 6 0.09215303554262277 2202 7 0.14156232596322837 2202 8 0.1244199326103228 2202 9 0.116413415084331 2202 10-11 0.1639553505991458 2202 12-13 0.1452712234399982 2202 14-15 0.20640342469172168 2202 16-17 0.24345716674788065 2202 18-19 0.2151852513517838 2202 20-21 0.25372164059131563 2202 22-23 0.24144277826272997 2202 24-25 0.25344723295226856 2202 26-27 0.2934410740633808 2202 28-29 0.2806358487191787 2202 30-31 0.30258552338005273 2202 32-33 0.3325605041379305 2202 34-35 0.2957568890321767 2202 36-37 0.31044841191050665 2202 38-39 0.2836152809192569 2202 40-41 0.28977532806985096 2202 42-43 0.37163912823873346 2202 44-45 0.32154880177649403 2202 46-47 0.2934777651370766 2202 48-49 0.390542671864452 2202 50-51 0.343372381736863 2202 52-53 0.395700986506597 2202 54-55 0.3498765883296713 2202 56-57 0.3620236138163264 2202 58-59 0.38211418853519064 2202 60-61 0.34516966153968553 2202 62-63 0.4146588854395503 2202 64-65 0.4171524556772468 2202 66-67 0.3361702934497117 2202 68-69 0.32704154928418916 2202 70-71 0.2659302616148693 2202 72-73 0.2726064180493566 2202 74-75 0.3383331760986721 2202 76-77 0.17479545828383536 2202 78-79 0.34392206056229213 2202 80-81 0.4114405493637392 2202 82-83 0.5060855781552789 2202 84-85 0.46816086807933033 2202 86-87 0.5237355810503246 2202 88-89 0.47824114986649846 2202 90-91 0.47274348245515085 2202 92-93 0.4286472817474447 2202 94-95 0.479054905100206 2202 96-97 0.552465959756212 2202 98-99 0.5290191202794006 2202 100-101 0.47626704024693467 1102 1 0.06822209966234283 1102 2 0.029679273095794656 1102 3 0.05430345022629979 1102 4 0.07197059951023022 1102 5 0.08395562095977738 1102 6 0.07510282079222463 1102 7 0.07848096214568301 1102 8 0.09088807096179607 1102 9 0.05613890100937624 1102 10-11 0.052141766102970166 1102 12-13 0.04192213531126043 1102 14-15 0.0670345954523981 1102 16-17 0.13396246624611763 1102 18-19 0.12190014138577965 1102 20-21 0.1492604818774197 1102 22-23 0.1326739680551512 1102 24-25 0.18330959439261818 1102 26-27 0.15027548922649459 1102 28-29 0.13772648818360977 1102 30-31 0.12019668663006655 1102 32-33 0.07411136847419186 1102 34-35 0.07473862742907755 1102 36-37 0.14453063038207858 1102 38-39 0.12806846981243325 1102 40-41 0.1371154697254866 1102 42-43 0.09926759418465991 1102 44-45 0.15494242263706326 1102 46-47 0.09292636578251745 1102 48-49 0.12454682801615036 1102 50-51 0.032487459914527506 1102 52-53 0.09605150445621291 1102 54-55 0.0862283535462538 1102 56-57 0.16212742888277631 1102 58-59 0.021483905035381667 1102 60-61 -0.12219175441330776 1102 62-63 0.11479604487833228 1102 64-65 0.10154187158675398 1102 66-67 0.05286634693557346 1102 68-69 0.0851088023267792 1102 70-71 0.10219830267818963 1102 72-73 -0.011984250805589625 1102 74-75 0.07277139690108214 1102 76-77 -0.09901152521333856 1102 78-79 0.10016521617653495 1102 80-81 -0.027492196706411676 1102 82-83 0.07957137876513798 1102 84-85 0.09139399897670586 1102 86-87 0.10048187124629493 1102 88-89 0.048254765993526405 1102 90-91 0.14544085197068313 1102 92-93 0.06492095691724842 1102 94-95 0.1489815252995399 1102 96-97 0.05465203189007184 1102 98-99 0.04791001314553256 1102 100-101 0.031917982506882936 2305 1 0.013777638152241423 2305 2 -0.016702010249808552 2305 3 0.035807598676946384 2305 4 0.022684853573998964 2305 5 0.05944376641751603 2305 6 0.018739749007814055 2305 7 0.04396950654716392 2305 8 0.0730021911512253 2305 9 0.043825720648975164 2305 10-11 0.02066731270864608 2305 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48-49 -0.30471790278354405 1213 50-51 -0.22612931906015632 1213 52-53 -0.18369793491595487 1213 54-55 -0.9973799129316774 1213 56-57 -0.9810922539224336 1213 58-59 -1.3218507273020492 1213 60-61 -0.6727837736761799 1213 62-63 -0.4134224833791009 1213 64-65 -0.7884234390491471 1213 66-67 -0.7603960297775814 1213 68-69 -0.2780588647775559 1213 70-71 -0.04980672488268567 1213 72-73 -0.03433291557514195 1213 74-75 0.010418177697651743 1213 76-77 -2.3164383308235195 1213 78-79 -1.9944063671995913 1213 80-81 -1.4190485217065358 1213 82-83 -1.0243052612776538 1213 84-85 -0.7065286661105432 1213 86-87 -0.43969129407983587 1213 88-89 -0.5585911580257417 1213 90-91 -1.7012584495925651 1213 92-93 -0.7973622135052523 1213 94-95 -0.4741120799252343 1213 96-97 -0.4894250778272138 1213 98-99 -2.408496214327023 1213 100-101 -0.8875567605047365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 6.0 10 14.0 11 22.0 12 53.0 13 169.0 14 547.0 15 1370.0 16 2975.0 17 4677.0 18 6203.0 19 8068.0 20 10226.0 21 13497.0 22 17797.0 23 22348.0 24 29020.0 25 37015.0 26 47726.0 27 62446.0 28 85024.0 29 114803.0 30 128743.0 31 148463.0 32 200677.0 33 288219.0 34 444835.0 35 751560.0 36 1391603.0 37 2198127.0 38 779485.0 39 338.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.03691711381563 27.639635693048593 20.160668510242576 16.1627786828932 2 30.627540906507 24.626046106301512 30.172007975210864 14.574405011980621 3 35.7043616809635 20.9457832885417 25.490559618782587 17.859295411712218 4 29.64353747422403 21.348773068152155 30.033439973584684 18.97424948403913 5 30.61191907432117 19.736420028122573 31.42145838324041 18.23020251431585 6 27.566097876151147 20.061982990727863 33.75397914496904 18.617939988151953 7 31.959821414119773 21.265930926428233 26.303439435037195 20.4708082244148 8 29.18441837900736 27.708136098897327 23.53293629924877 19.57450922284654 9 28.04811259703787 24.815429768256834 26.629966960258432 20.506490674446866 10-11 22.76575202366025 29.27161473973187 30.86193804494515 17.100695191662734 12-13 22.137914361531347 28.190687895836085 30.75398267642801 18.917415066204555 14-15 21.647137208069736 28.3513177786299 31.344825191309432 18.656719821990926 16-17 21.91499837111455 27.72919978022554 31.943517845197906 18.412284003462005 18-19 20.93777318230262 27.78787023122378 32.52167396477104 18.752682621702554 20-21 21.659828388751244 28.1491049664379 31.917267333504217 18.273799311306643 22-23 21.076865441701646 29.703748849700812 30.68344178345741 18.535943925140135 24-25 21.062504175214514 28.12905657956421 31.398267937089415 19.41017130813186 26-27 21.11727857531528 28.27241909942499 30.64546388509339 19.964838440166343 28-29 21.676720534168485 29.08146899276021 30.416044124402703 18.825766348668598 30-31 20.8688551510302 28.66714792604772 30.9869706821219 19.477026240800182 32-33 20.75717276103294 29.36283798637386 30.363373885272903 19.51661536732029 34-35 21.54646855574217 29.216091151664685 30.514417328398313 18.723022964194833 36-37 21.430345566554255 29.055590616735554 30.720114619403578 18.793949197306613 38-39 21.09270596461531 28.48192169387906 31.54277501947456 18.882597322031074 40-41 21.002873211636725 28.65421821649874 29.89323141732183 20.449677154542705 42-43 20.778984103763115 29.16018213415741 30.763983322786657 19.29685043929282 44-45 20.69780548026639 28.389962066032766 30.935238566101926 19.97699388759892 46-47 21.415880070306414 28.48272833269187 30.069566974261523 20.031824622740192 48-49 20.866447358788516 29.484318451755342 30.278996202566198 19.37023798688994 50-51 21.585655122725832 28.78430958781184 30.068517270145755 19.56151801931657 52-53 20.800760765400625 28.752296919231213 29.434646095099353 21.012296220268805 54-55 21.441852254207667 28.89222040487559 29.996918924141585 19.669008416775153 56-57 20.719288956438767 28.793411571631484 30.194694633414702 20.292604838515047 58-59 20.73750647561636 28.86971795747286 30.324560835472248 20.06821473143853 60-61 21.201511576136497 28.906954805686503 30.276420593948266 19.615113024228734 62-63 20.319049099333583 28.83245011064043 30.29067438237258 20.557826407653412 64-65 21.102026012945768 28.71339131107893 30.16752944562947 20.017053230345834 66-67 20.527243975762854 29.799116169728322 29.606989393433263 20.06665046107556 68-69 20.4860073873747 30.013930902751788 29.505753590156804 19.994308119716713 70-71 20.267131772721758 30.33230216310232 29.352501539689847 20.048064524486072 72-73 20.28241792297649 30.212828685452482 29.339881425185112 20.16487196638592 74-75 20.39515576577566 30.052315916185023 29.363871901810583 20.18865641622874 76-77 20.48747577369975 29.88608799878359 29.36651509114329 20.25992113637337 78-79 20.31486046190935 29.799276897041494 29.636558863485952 20.249303777563206 80-81 20.243211240654848 30.031262953576586 29.480931378802786 20.244594426965776 82-83 20.109517899935522 29.893645698727116 29.495635558142673 20.501200843194685 84-85 20.259848464893658 29.610377398610556 29.627498637732092 20.502275498763694 86-87 20.333157744688222 29.59147013028148 29.73396480485302 20.341407320177286 88-89 20.53275154289711 29.849692362558116 29.338050004128526 20.27950609041625 90-91 20.590849917541963 29.70825824046839 29.278063512925783 20.422828329063865 92-93 20.33711304416432 29.836699093592127 29.236443164759397 20.589744697484154 94-95 20.093327432967918 29.908942976226054 29.194511070507872 20.80321852029816 96-97 20.119005213561735 30.18722162200353 29.06773361672814 20.626039547706593 98-99 20.39493629073338 30.082884128925436 28.87970153970777 20.64247804063341 100-101 20.48128790487397 29.984758387210558 28.63999201675363 20.893961691161845 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 139.0 1 87.0 2 32.5 3 30.0 4 41.0 5 42.0 6 37.5 7 46.5 8 62.5 9 74.5 10 89.5 11 119.0 12 136.0 13 145.5 14 169.0 15 205.5 16 263.0 17 303.0 18 364.0 19 515.0 20 773.0 21 1193.5 22 2015.0 23 3395.5 24 5591.0 25 9245.5 26 15873.5 27 25265.5 28 36265.0 29 49896.5 30 66356.0 31 85063.5 32 110117.5 33 144778.0 34 186669.0 35 233289.5 36 288228.5 37 348983.0 38 403451.5 39 437030.5 40 455622.5 41 451348.5 42 425753.0 43 404350.5 44 374004.0 45 343013.5 46 324634.5 47 296475.0 48 255225.0 49 216202.0 50 177590.5 51 141989.5 52 114068.5 53 90585.5 54 73463.5 55 57999.0 56 41226.0 57 27842.0 58 19573.5 59 14550.5 60 10547.0 61 7298.5 62 5084.0 63 3379.0 64 2319.5 65 1625.5 66 1090.0 67 764.5 68 561.5 69 401.0 70 295.5 71 210.0 72 154.5 73 124.0 74 93.5 75 74.5 76 47.5 77 35.0 78 33.5 79 24.0 80 14.5 81 10.0 82 10.0 83 7.0 84 4.5 85 3.5 86 3.0 87 4.0 88 2.0 89 1.0 90 2.5 91 2.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17332106347532641 2 0.007371920604562232 3 0.0021483042081159405 4 0.0 5 0.0013831547641294409 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.7221662911058143E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.4714412384355756E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.4714412384355756E-5 38-39 1.7657294861226906E-4 40-41 6.62148557296009E-5 42-43 0.0011550813721719268 44-45 5.591476706055187E-4 46-47 0.005539976262709941 48-49 0.004186250323349212 50-51 0.008019354749473885 52-53 0.003980248549968232 54-55 0.019915957162225516 56-57 0.011035809288266816 58-59 0.019687883770268002 60-61 0.009336294657873726 62-63 0.005819550098012701 64-65 0.01272060950627555 66-67 0.030995909687645398 68-69 0.03192291766785981 70-71 0.036521171537970985 72-73 0.03118719704864202 74-75 0.034314009680317614 76-77 0.03391672054594001 78-79 0.01728943455161801 80-81 0.002280733919575142 82-83 0.0 84-85 0.005959337015664081 86-87 0.02602979550792533 88-89 0.027354092622517347 90-91 0.024911500166714295 92-93 0.03490994338188403 94-95 0.03131962676010122 96-97 0.032930854916188176 98-99 0.03911826532380978 100-101 0.026920017457178855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6796058.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.2749343612588 #Duplication Level Percentage of deduplicated Percentage of total 1 91.8600547551961 60.88019099322259 2 4.785971480234853 6.343798914148429 3 0.9104358167256512 1.8101722198089472 4 0.6012542908063868 1.5939235463047399 5 0.29898745158771245 0.9907686864407841 6 0.25538886606834993 1.0155528201165722 7 0.15906347861202358 0.7379345122999749 8 0.12556716421301553 0.6657564452917607 9 0.13401995225769817 0.7993947185080185 >10 0.7298779208137837 9.909199706974487 >50 0.07663455379062854 3.549905160557395 >100 0.05668483603780428 7.523103146931925 >500 0.0051170962772722015 2.2813796532031443 >1k 9.20144996698736E-4 0.7823185470740787 >5k 0.0 0.0 >10k+ 2.2192382042482018E-5 1.1166009291171481 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 75918 1.11708875939552 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.564368049831241E-4 0.0 0.0 2 0.0010005800421361913 0.0 1.4714412384355754E-5 3 0.00107415210405797 0.0 1.4714412384355754E-5 4 0.0012360106402858834 0.0 1.4714412384355754E-5 5 0.0013978691765137966 0.0 1.4714412384355754E-5 6 0.0016627285994322003 0.0 1.4714412384355754E-5 7 0.001986445671888027 0.0 1.4714412384355754E-5 8 0.002457306868187411 0.0 1.4714412384355754E-5 9 0.002751595115874526 0.0 1.4714412384355754E-5 10-11 0.004826327262068688 0.0 1.4714412384355754E-5 12-13 0.006753915284419291 0.0 1.4714412384355754E-5 14-15 0.009615868493176485 0.0 1.4714412384355754E-5 16-17 0.014250908394248548 0.0 1.4714412384355754E-5 18-19 0.019798241863150667 0.0 1.4714412384355754E-5 20-21 0.022410050061373813 0.0 2.9428824768711508E-5 22-23 0.0248084992800238 0.0 2.9428824768711508E-5 24-25 0.027383521447286056 7.357206192177877E-6 3.6786030960889387E-5 26-27 0.03009097332600752 1.4714412384355754E-5 5.150044334524514E-5 28-29 0.03347528817440934 1.4714412384355754E-5 5.8857649537423016E-5 30-31 0.052942455758912 1.4714412384355754E-5 5.8857649537423016E-5 32-33 0.05791592714482425 1.4714412384355754E-5 5.8857649537423016E-5 34-35 0.061888818488600304 1.4714412384355754E-5 5.8857649537423016E-5 36-37 0.06598678233764338 1.4714412384355754E-5 5.8857649537423016E-5 38-39 0.07077632356875119 1.4714412384355754E-5 5.8857649537423016E-5 40-41 0.07626479938811587 1.4714412384355754E-5 6.62148557296009E-5 42-43 0.08216527875424254 1.4714412384355754E-5 7.357206192177877E-5 44-45 0.08804368650179266 1.4714412384355754E-5 7.357206192177877E-5 46-47 0.09520224812678173 1.4714412384355754E-5 7.357206192177877E-5 48-49 0.10320688846387126 1.4714412384355754E-5 1.0300088669049029E-4 50-51 0.1119766782449473 1.4714412384355754E-5 1.324297114592018E-4 52-53 0.12143804540808803 1.4714412384355754E-5 1.324297114592018E-4 54-55 0.13087734095265224 1.4714412384355754E-5 1.4714412384355755E-4 56-57 0.14156000434369453 1.4714412384355754E-5 1.618585362279133E-4 58-59 0.15346396396263834 1.4714412384355754E-5 1.618585362279133E-4 60-61 0.16623607391225914 1.4714412384355754E-5 1.7657294861226906E-4 62-63 0.18061941201796688 1.4714412384355754E-5 1.7657294861226906E-4 64-65 0.1964079765063806 1.4714412384355754E-5 1.7657294861226906E-4 66-67 0.21441106005863986 1.4714412384355754E-5 1.8393015480444694E-4 68-69 0.2343270172208654 1.4714412384355754E-5 1.9128736099662482E-4 70-71 0.2576346464376849 1.4714412384355754E-5 1.9128736099662482E-4 72-73 0.28331865325457783 1.4714412384355754E-5 1.9128736099662482E-4 74-75 0.3113201800220069 1.4714412384355754E-5 2.0600177338098058E-4 76-77 0.34461153804161176 1.4714412384355754E-5 2.1335897957315845E-4 78-79 0.3831117980452786 1.4714412384355754E-5 2.3543059814969206E-4 80-81 0.42579095116610244 1.4714412384355754E-5 2.3543059814969206E-4 82-83 0.47271521225981294 1.4714412384355754E-5 2.3543059814969206E-4 84-85 0.527151180875737 1.4714412384355754E-5 2.4278780434186994E-4 86-87 0.5878039887240515 1.4714412384355754E-5 2.5750221672622567E-4 88-89 0.6565791522085302 1.4714412384355754E-5 2.648594229184036E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 15240 0.0 56.24989 3 CGGAAGA 15350 0.0 55.845566 4 GATCGGA 15825 0.0 54.172543 1 ATCGGAA 15990 0.0 53.61468 2 GAAGAGC 17865 0.0 48.115948 6 TATGCCG 10010 0.0 42.065884 48-49 TGCCGTC 10120 0.0 41.751297 50-51 GCCGTCT 10230 0.0 41.604176 50-51 ATGCCGT 10205 0.0 41.378437 48-49 CGTATGC 10240 0.0 41.18865 46-47 CTCGTAT 10270 0.0 40.651505 44-45 TCTCGTA 10295 0.0 40.55249 42-43 TCGTATG 10590 0.0 40.208004 44-45 CCGTCTT 10540 0.0 40.133236 52-53 CATCTCG 10435 0.0 39.55181 40-41 AGAGCAC 21655 0.0 39.519375 8 GTATGCC 10830 0.0 39.25176 46-47 CGTCTTC 10900 0.0 39.199955 52-53 ATCTCGT 10600 0.0 38.95998 42-43 GAGCACA 22065 0.0 38.720478 9 >>END_MODULE