FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_35010-1.341000000357eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_35010-1.341000000357eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6796058
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT759181.11708875939552TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG152400.056.249893
CGGAAGA153500.055.8455664
GATCGGA158250.054.1725431
ATCGGAA159900.053.614682
GAAGAGC178650.048.1159486
TATGCCG100100.042.06588448-49
TGCCGTC101200.041.75129750-51
GCCGTCT102300.041.60417650-51
ATGCCGT102050.041.37843748-49
CGTATGC102400.041.1886546-47
CTCGTAT102700.040.65150544-45
TCTCGTA102950.040.5524942-43
TCGTATG105900.040.20800444-45
CCGTCTT105400.040.13323652-53
CATCTCG104350.039.5518140-41
AGAGCAC216550.039.5193758
GTATGCC108300.039.2517646-47
CGTCTTC109000.039.19995552-53
ATCTCGT106000.038.9599842-43
GAGCACA220650.038.7204789
Produced by FastQC (version 0.11.2)