##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_35008-2.341000000358b3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5737772 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.694711117834586 33.0 33.0 33.0 33.0 33.0 2 32.71797746581774 33.0 33.0 33.0 33.0 33.0 3 32.74958834195573 33.0 33.0 33.0 33.0 33.0 4 36.69124112983228 37.0 37.0 37.0 37.0 37.0 5 36.65512728634041 37.0 37.0 37.0 37.0 37.0 6 36.539095314348494 37.0 37.0 37.0 37.0 37.0 7 36.46909758700764 37.0 37.0 37.0 37.0 37.0 8 36.40553563299483 37.0 37.0 37.0 37.0 37.0 9 36.44794773999385 37.0 37.0 37.0 37.0 37.0 10-11 36.37831165128206 37.0 37.0 37.0 37.0 37.0 12-13 36.35306622500859 37.0 37.0 37.0 37.0 37.0 14-15 38.202953167187545 40.0 37.0 40.0 37.0 40.0 16-17 38.19841455882179 40.0 37.0 40.0 37.0 40.0 18-19 38.168631047033585 40.0 37.0 40.0 37.0 40.0 20-21 38.120668004932924 40.0 37.0 40.0 37.0 40.0 22-23 37.98749802188027 40.0 37.0 40.0 33.0 40.0 24-25 37.98557427865729 40.0 37.0 40.0 33.0 40.0 26-27 37.91497666690137 40.0 37.0 40.0 33.0 40.0 28-29 37.751050582002904 40.0 37.0 40.0 33.0 40.0 30-31 37.69994930087846 40.0 37.0 40.0 33.0 40.0 32-33 37.56796575395467 40.0 37.0 40.0 33.0 40.0 34-35 37.43239213757535 40.0 37.0 40.0 33.0 40.0 36-37 37.24844477961132 40.0 37.0 40.0 33.0 40.0 38-39 37.284663280451014 40.0 37.0 40.0 33.0 40.0 40-41 37.11618237880487 40.0 37.0 40.0 33.0 40.0 42-43 36.98317639669196 38.5 37.0 40.0 33.0 40.0 44-45 36.96002925874364 38.5 37.0 40.0 33.0 40.0 46-47 36.89065250414272 37.0 37.0 40.0 33.0 40.0 48-49 36.640344370602385 37.0 37.0 40.0 33.0 40.0 50-51 36.54610918663202 37.0 37.0 40.0 33.0 40.0 52-53 36.45639230697908 37.0 37.0 40.0 33.0 40.0 54-55 36.22461645391277 37.0 37.0 40.0 33.0 40.0 56-57 36.070720830315324 37.0 37.0 40.0 30.0 40.0 58-59 35.853433963566346 37.0 37.0 40.0 27.0 40.0 60-61 35.6375903399438 37.0 35.0 40.0 27.0 40.0 62-63 35.450042978354666 37.0 33.0 40.0 27.0 40.0 64-65 35.19344172267563 37.0 33.0 40.0 27.0 40.0 66-67 34.777071309212005 37.0 33.0 40.0 27.0 40.0 68-69 34.54355523363424 37.0 33.0 37.0 27.0 40.0 70-71 34.219855372433756 37.0 33.0 37.0 27.0 40.0 72-73 33.68856962249458 37.0 33.0 37.0 27.0 40.0 74-75 33.065871125586725 37.0 33.0 37.0 22.0 40.0 76-77 30.46575229897598 33.0 30.0 35.0 22.0 37.0 78-79 32.66854216235849 35.0 33.0 37.0 22.0 37.0 80-81 32.94452498286792 37.0 33.0 37.0 22.0 37.0 82-83 32.80772353798652 37.0 33.0 37.0 22.0 37.0 84-85 32.62581564760677 37.0 33.0 37.0 22.0 37.0 86-87 32.42269342525287 37.0 33.0 37.0 22.0 37.0 88-89 32.22798701307755 37.0 33.0 37.0 22.0 37.0 90-91 32.04742677122758 33.0 33.0 37.0 22.0 37.0 92-93 31.868895801366804 33.0 33.0 37.0 22.0 37.0 94-95 31.754426021110632 33.0 33.0 37.0 18.5 37.0 96-97 31.61101051069997 33.0 33.0 37.0 15.0 37.0 98-99 31.304306619363754 33.0 33.0 37.0 15.0 37.0 100-101 29.68701309497833 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12410618439164267 2306 2 0.12044359483728329 2306 3 0.09634419566029351 2306 4 0.17106600393762506 2306 5 0.17351722822093762 2306 6 0.20329628247218778 2306 7 0.25334476187388333 2306 8 0.24562555479950987 2306 9 0.1901454880490121 2306 10-11 0.23907030185736033 2306 12-13 0.2345466800883571 2306 14-15 0.45367565337086546 2306 16-17 0.4779234487735238 2306 18-19 0.4411281436740211 2306 20-21 0.43081100173758813 2306 22-23 0.5360586999613091 2306 24-25 0.47607995097159517 2306 26-27 0.5153375653302774 2306 28-29 0.509635869360956 2306 30-31 0.5838039699913224 2306 32-33 0.5232078262044482 2306 34-35 0.5965560232609235 2306 36-37 0.5325106442618974 2306 38-39 0.5591812636207649 2306 40-41 0.5960503294505486 2306 42-43 0.7076854160224144 2306 44-45 0.6560716458222373 2306 46-47 0.5036978003081884 2306 48-49 0.5628538906638383 2306 50-51 0.5174011606878182 2306 52-53 0.48808453337431956 2306 54-55 0.4755418477968334 2306 56-57 0.40812346450985615 2306 58-59 0.46711734540465955 2306 60-61 0.3787137344212823 2306 62-63 0.5022115342847968 2306 64-65 0.32537103101486053 2306 66-67 0.4278386702032506 2306 68-69 0.5230285926477336 2306 70-71 0.38952824399338937 2306 72-73 0.4233375895983116 2306 74-75 0.3217870497412747 2306 76-77 0.0347403619101847 2306 78-79 0.3947802319136642 2306 80-81 0.4516875925035322 2306 82-83 0.4123388269692967 2306 84-85 0.3874175476581314 2306 86-87 0.34155784482193496 2306 88-89 0.39146716890410715 2306 90-91 0.5017325293056416 2306 92-93 0.5012524662446118 2306 94-95 0.6878952637421634 2306 96-97 0.5663711367244595 2306 98-99 0.5201183745657545 2306 100-101 0.17452819953727072 2307 1 0.1134278747631754 2307 2 0.06433246032443662 2307 3 0.13504339443931457 2307 4 0.15363062293071295 2307 5 0.17922357258367327 2307 6 0.23649655245201728 2307 7 0.21876324530430935 2307 8 0.2358122386027759 2307 9 0.1963473178905346 2307 10-11 0.2212730211824976 2307 12-13 0.20464791599384569 2307 14-15 0.47152537570111974 2307 16-17 0.44333272389335576 2307 18-19 0.3950387531825541 2307 20-21 0.5127488678454881 2307 22-23 0.5455219697409674 2307 24-25 0.49480972003565427 2307 26-27 0.4703905604639118 2307 28-29 0.5302088352631031 2307 30-31 0.5782398972926899 2307 32-33 0.5601790299886034 2307 34-35 0.5068646616685228 2307 36-37 0.5532144857274446 2307 38-39 0.48505841234901936 2307 40-41 0.5776240754468205 2307 42-43 0.5024713897408546 2307 44-45 0.4499147473118583 2307 46-47 0.5239724757234114 2307 48-49 0.5717849070294889 2307 50-51 0.5030909235607552 2307 52-53 0.50101571170174 2307 54-55 0.5676596971926244 2307 56-57 0.4747331180194152 2307 58-59 0.47860265570023586 2307 60-61 0.5730134962786977 2307 62-63 0.38433771360979563 2307 64-65 0.3759681834264441 2307 66-67 0.5122000852920152 2307 68-69 0.5533484900109897 2307 70-71 0.40921809372485995 2307 72-73 0.45899393152835444 2307 74-75 0.37986301569142 2307 76-77 0.08186160564600442 2307 78-79 0.49703359041637185 2307 80-81 0.5822696981437545 2307 82-83 0.5455570237665626 2307 84-85 0.5296699787990775 2307 86-87 0.5502281876290525 2307 88-89 0.5923026434961898 2307 90-91 0.7172780841569661 2307 92-93 0.6958406572066913 2307 94-95 0.6770272041389234 2307 96-97 0.7148063280140526 2307 98-99 0.5661309964819914 2307 100-101 0.42254957196086096 2201 1 0.06905223917743086 2201 2 0.07841822062420789 2201 3 0.0878902386649969 2201 4 0.1184936912950505 2201 5 0.144691967580016 2201 6 0.1394635589190898 2201 7 0.14377487432714275 2201 8 0.1955271453394829 2201 9 0.1514132699293924 2201 10-11 0.19709538684167427 2201 12-13 0.19666129757865036 2201 14-15 0.41370589148903747 2201 16-17 0.4307992022720839 2201 18-19 0.3604565407950773 2201 20-21 0.2609698625004313 2201 22-23 0.31986843481521277 2201 24-25 0.3327713111664252 2201 26-27 0.3744854999159628 2201 28-29 0.35093050463508035 2201 30-31 0.43918254954776614 2201 32-33 0.35115178065341723 2201 34-35 0.35359523713218266 2201 36-37 0.4812467177260018 2201 38-39 0.4614731209898437 2201 40-41 0.42659796601842004 2201 42-43 0.4799745899221364 2201 44-45 0.45992851748290065 2201 46-47 0.45056591729498763 2201 48-49 0.531808363003698 2201 50-51 0.5026780146269871 2201 52-53 0.42755493931996114 2201 54-55 0.46304289578355906 2201 56-57 0.5093217068218507 2201 58-59 0.4298888363009681 2201 60-61 0.5945366665837355 2201 62-63 0.5887278955003481 2201 64-65 0.41467957170932124 2201 66-67 0.4265203418581791 2201 68-69 0.5142358203745516 2201 70-71 0.4593343204775593 2201 72-73 0.4238521238106756 2201 74-75 0.30129213820382006 2201 76-77 0.05799803138093651 2201 78-79 0.3358348197891772 2201 80-81 0.3712610860596257 2201 82-83 0.4174343332333663 2201 84-85 0.31356052327970474 2201 86-87 0.483852189308962 2201 88-89 0.4947252310776875 2201 90-91 0.4689792741575758 2201 92-93 0.49167890100467204 2201 94-95 0.6275462818242659 2201 96-97 0.624872015474967 2201 98-99 0.592769551910667 2201 100-101 0.5749298472684927 1101 1 0.13867127069534035 1101 2 0.11455361724211599 1101 3 0.13619226012679775 1101 4 0.15412091257648086 1101 5 0.17624347370504978 1101 6 0.1069397644696366 1101 7 0.14492164121846685 1101 8 0.13884822558993903 1101 9 0.18003837986390892 1101 10-11 0.16459695688836717 1101 12-13 0.16296969464353595 1101 14-15 0.3789924951913406 1101 16-17 0.4063094166640582 1101 18-19 0.38346797210994765 1101 20-21 0.39565354015029186 1101 22-23 0.41233327321733526 1101 24-25 0.3948306030459463 1101 26-27 0.3688644254884039 1101 28-29 0.37340555758541427 1101 30-31 0.4163020292412227 1101 32-33 0.4589760714657416 1101 34-35 0.456680637984455 1101 36-37 0.43563140577863635 1101 38-39 0.41898253788455264 1101 40-41 0.5250546848651112 1101 42-43 0.5337354988690137 1101 44-45 0.5290635662895014 1101 46-47 0.4810265677948422 1101 48-49 0.5467560980791859 1101 50-51 0.5068809689293303 1101 52-53 0.5856729634975864 1101 54-55 0.5031163257394766 1101 56-57 0.5801780778364005 1101 58-59 0.5763136715072648 1101 60-61 0.4027357113161898 1101 62-63 0.5361867740271151 1101 64-65 0.5171421622024965 1101 66-67 0.4560747715148281 1101 68-69 0.5321352640535508 1101 70-71 0.5373357710975739 1101 72-73 0.482832676273361 1101 74-75 0.3085548476132516 1101 76-77 0.29835965305452916 1101 78-79 0.5781876585762973 1101 80-81 0.4404001192275331 1101 82-83 0.5239064932769821 1101 84-85 0.31972789791268497 1101 86-87 0.4185142889079927 1101 88-89 0.589610061161089 1101 90-91 0.4992565919542926 1101 92-93 0.5775834494577801 1101 94-95 0.6347674288765717 1101 96-97 0.5261383704968701 1101 98-99 0.5577126932519505 1101 100-101 0.6383000981832545 2304 1 0.09290569534255155 2304 2 0.09445822415071348 2304 3 0.09011556520855635 2304 4 0.10066738676609788 2304 5 0.10012950925553099 2304 6 0.15368731095087185 2304 7 0.0754329171891257 2304 8 0.13347563595636558 2304 9 0.15023233642271805 2304 10-11 0.1751869990895898 2304 12-13 0.1547734976856887 2304 14-15 0.2654819730656328 2304 16-17 0.33399250587844165 2304 18-19 0.3619405554027679 2304 20-21 0.2902949706239468 2304 22-23 0.35723157460031274 2304 24-25 0.31994554678938414 2304 26-27 0.37864707859995406 2304 28-29 0.3577422796984422 2304 30-31 0.4132430391116628 2304 32-33 0.39987723323242363 2304 34-35 0.35978964960211357 2304 36-37 0.3239451180071313 2304 38-39 0.41248002624223545 2304 40-41 0.39277201414036256 2304 42-43 0.4146623759077386 2304 44-45 0.41265842041698164 2304 46-47 0.4486768260043448 2304 48-49 0.5187125723392612 2304 50-51 0.4800082982750311 2304 52-53 0.4977824612395736 2304 54-55 0.40600792001588815 2304 56-57 0.5114574302342589 2304 58-59 0.5461455711261181 2304 60-61 0.5270857490084353 2304 62-63 0.3784243549597761 2304 64-65 0.3484065081539285 2304 66-67 0.286346648870591 2304 68-69 0.4181031093955596 2304 70-71 0.31975948832821643 2304 72-73 0.28380555899058635 2304 74-75 0.3122514355841446 2304 76-77 0.07209865361110701 2304 78-79 0.3214026312053804 2304 80-81 0.176324283833452 2304 82-83 0.2210346257846112 2304 84-85 0.19847891067168888 2304 86-87 0.30732314341592115 2304 88-89 0.3711391687354606 2304 90-91 0.35972742801767765 2304 92-93 0.2294444601736565 2304 94-95 0.3780586314354082 2304 96-97 0.32724046364956294 2304 98-99 0.5231871576345348 2304 100-101 0.36130195109189955 2202 1 0.06855718008930012 2202 2 0.05156297591094727 2202 3 0.05784176440270272 2202 4 0.09920644795344913 2202 5 0.10586357679906655 2202 6 0.09464552500674728 2202 7 0.07395574713196851 2202 8 0.09942020245215843 2202 9 0.10427813262548824 2202 10-11 0.10232342079761025 2202 12-13 0.13307479650227094 2202 14-15 0.15769186334622987 2202 16-17 0.14454173695493466 2202 18-19 0.1444498677622832 2202 20-21 0.22235694100826464 2202 22-23 0.21877309900583697 2202 24-25 0.22255281724566345 2202 26-27 0.28073095482402977 2202 28-29 0.19761604373038466 2202 30-31 0.2826650863407849 2202 32-33 0.22813646396019038 2202 34-35 0.21196910629924304 2202 36-37 0.27300741491843894 2202 38-39 0.32427411768880887 2202 40-41 0.23606625933994962 2202 42-43 0.38224040159241923 2202 44-45 0.3454934672244647 2202 46-47 0.28481531148975137 2202 48-49 0.3145402548535756 2202 50-51 0.25618932737398126 2202 52-53 0.3775665096036107 2202 54-55 0.35041964835811257 2202 56-57 0.3734955292765534 2202 58-59 0.2771533348600599 2202 60-61 0.3547166530443775 2202 62-63 0.43391384427790314 2202 64-65 0.28086449554301396 2202 66-67 0.3344895979482345 2202 68-69 0.2904067731551834 2202 70-71 0.31023041633060444 2202 72-73 0.15179114108042313 2202 74-75 0.15437449504692324 2202 76-77 0.03016827677360112 2202 78-79 0.18698015600399742 2202 80-81 0.19625523764098318 2202 82-83 0.3973449976132599 2202 84-85 0.3680026567921999 2202 86-87 0.4011436676977951 2202 88-89 0.3744512302620322 2202 90-91 0.35085483377659443 2202 92-93 0.3325572451318557 2202 94-95 0.33695287790305883 2202 96-97 0.4508619332746271 2202 98-99 0.38746409056335906 2202 100-101 0.367185650420776 1102 1 0.0510543734912261 1102 2 0.06380031349280557 1102 3 0.06896582405881446 1102 4 0.07170059779930682 1102 5 0.11239316151918644 1102 6 0.11632547358902912 1102 7 0.09680049855671058 1102 8 0.09053787301859728 1102 9 0.08550951169988963 1102 10-11 0.07873416263675637 1102 12-13 0.05585515876735059 1102 14-15 0.11938544696910469 1102 16-17 0.1787527906387254 1102 18-19 0.186046159508372 1102 20-21 0.18128020160918368 1102 22-23 0.17194566931441813 1102 24-25 0.10693409377792307 1102 26-27 0.1662567262095962 1102 28-29 0.15563025758642368 1102 30-31 0.16395382982245366 1102 32-33 0.177818891174077 1102 34-35 0.1597257095381721 1102 36-37 0.15981419387620122 1102 38-39 0.1285366022988086 1102 40-41 0.21474445611281112 1102 42-43 0.16562604687140947 1102 44-45 0.19492958268813965 1102 46-47 0.12685554418307277 1102 48-49 0.18237025599693624 1102 50-51 0.1684324666992083 1102 52-53 0.2538901573472714 1102 54-55 0.21651375052171318 1102 56-57 0.21313887191570302 1102 58-59 0.13059663557717727 1102 60-61 0.00892131084400205 1102 62-63 0.19109440762982643 1102 64-65 0.1987910785384983 1102 66-67 0.2451977477216971 1102 68-69 0.35594780724025554 1102 70-71 0.3156604442291737 1102 72-73 0.15224678743181386 1102 74-75 -0.01233242899971998 1102 76-77 -0.0032387617263083257 1102 78-79 0.21929548909823637 1102 80-81 0.1584038259129983 1102 82-83 0.2699574547074448 1102 84-85 0.22149729369007787 1102 86-87 0.2303798264726069 1102 88-89 0.2807597183560162 1102 90-91 0.4073825556727968 1102 92-93 0.32114555187475347 1102 94-95 0.2957368891136696 1102 96-97 0.27647283288193236 1102 98-99 0.2611377155850967 1102 100-101 0.06838059817054543 2305 1 -4.2691293416652343E-4 2305 2 0.007800334485253302 2305 3 0.023695303310475424 2305 4 0.017810053104582835 2305 5 0.04825537145279668 2305 6 0.014505251141329722 2305 7 0.020115256527077463 2305 8 0.03986956685083243 2305 9 -0.018445942598155796 2305 10-11 0.014930462608752748 2305 12-13 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13 202.0 14 565.0 15 1344.0 16 2663.0 17 4597.0 18 6880.0 19 9491.0 20 12881.0 21 16756.0 22 21874.0 23 27612.0 24 34973.0 25 44834.0 26 57507.0 27 79198.0 28 118163.0 29 161284.0 30 144076.0 31 141195.0 32 182964.0 33 255319.0 34 379055.0 35 607910.0 36 1042879.0 37 1636139.0 38 742912.0 39 4316.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.21994514172942 27.11140705301168 19.229428279471655 16.439219525787248 2 29.523862974241133 27.51880946832565 28.137225152216082 14.820102405217133 3 33.65603646631965 21.670543380092084 27.67570783860649 16.997712314981776 4 29.199783469960117 22.245655630791884 27.956112581678045 20.598448317569957 5 33.01328750792998 20.5605710979274 29.23948885620072 17.186652537941903 6 30.428675102461373 21.26860391106513 30.804343567503206 17.49837741897029 7 30.8691422384856 25.023754865128833 24.97049377354137 19.1366091228442 8 28.51057867060594 30.520853739047144 22.712108463006196 18.256459127340715 9 30.982513770153293 25.23406297775513 24.624784672517485 19.15863857957409 10-11 26.14010559525167 29.879397144320137 27.765531414883654 16.214965845544548 12-13 24.10782443080694 29.51107154484354 27.13719715596925 19.24390686838027 14-15 23.324037912628622 29.79398883346958 27.669976052549323 19.211997201352478 16-17 24.79675909046229 27.822924995974045 27.622699194042568 19.757616719521096 18-19 22.03311565294531 28.224737136553053 29.88817955407763 19.85396765642401 20-21 23.699669488435582 28.02562562611411 30.08341913899681 18.191285746453502 22-23 21.649143604869625 32.48513534521762 27.53383194731335 18.331889102599405 24-25 21.225276989047316 28.31311526494953 30.087575804685162 20.374031941317988 26-27 21.40225334851228 28.121899929101403 27.80929601245919 22.666550709927126 28-29 23.092395445479536 30.67953031246275 27.711514155668787 18.516560086388935 30-31 20.91072980941034 28.36839804718626 30.029565482908698 20.691306660494703 32-33 20.99544910463504 30.649797517224453 27.618298531206886 20.73645484693362 34-35 22.734643342398407 30.5620021151067 28.126257021017913 18.577097521476976 36-37 20.87302535487863 30.224094842396564 29.96518770397183 18.937692098752972 38-39 20.72518569099638 28.62083935920616 29.850195843045313 20.80377910675215 40-41 22.641981808781587 28.26750504137628 30.06327285598922 19.027240293852916 42-43 20.660562578810097 30.32634241858868 29.79722658180085 19.215868420800376 44-45 20.360543123960554 28.229679581611727 30.296306075614364 21.113471218813356 46-47 22.620740657208717 28.060701029720352 27.89039834288218 21.42815997018875 48-49 20.401385999646713 30.512454031746643 29.844735168220343 19.2414248003863 50-51 22.39391950281788 28.112582342825732 30.05336662196499 19.4401315323914 52-53 20.51797394278512 28.237953697132284 27.741633679173333 23.50243868090927 54-55 22.237108090725684 28.237958941441306 30.232734080040107 19.292198887792907 56-57 20.443885200816997 28.04194757154904 29.92318960369273 21.590977623941235 58-59 20.23149756968604 28.58266593069123 29.876340612290775 21.30949588733196 60-61 22.17990388172633 28.154104722641392 30.156936242009934 19.509055153622345 62-63 20.231013786455605 28.382353594781897 29.794838486692548 21.591794132069953 64-65 22.065413988408082 28.361720194362416 30.110504339844358 19.462361477385144 66-67 20.31582073528731 32.01934004076551 27.82171053554884 19.843128688398338 68-69 20.17035212009169 32.4578216714285 27.77195284981356 19.59987335866625 70-71 20.06820532557713 32.14767010904222 28.041664178561266 19.742460386819392 72-73 20.131568865492902 32.253139703638375 27.733760418861348 19.88153101200738 74-75 19.968893376914917 31.959875908249934 28.182082710631345 19.889148004203804 76-77 20.27913720214063 31.464701017638134 27.93961032162749 20.316551458593743 78-79 20.126653736566745 31.582970373348573 28.06041441455418 20.2299614755305 80-81 20.133595544421627 31.020912603691798 28.30548190176182 20.54000995012476 82-83 20.232043029942634 31.177031781674142 27.93160829673957 20.659316891643655 84-85 20.116740920612987 30.917807709425453 28.42089512411141 20.54455624585015 86-87 20.36322136163905 30.476943335483703 28.261584510304814 20.898250792572433 88-89 20.32667352956815 30.901405209587256 28.16135547243755 20.610565788407044 90-91 20.356753201393825 30.378726460941962 28.367897337137997 20.89662300052621 92-93 20.398426038790774 30.51147288496238 28.04781509974468 21.042285976502164 94-95 20.17154875141662 30.431636909890926 28.457071537166527 20.93974280152593 96-97 20.376676086983053 30.245692428122872 28.16019651907396 21.217434965820118 98-99 20.27383770489488 30.458756353696252 28.12271976993574 21.144686171473126 100-101 20.4114753919742 30.161731986620033 28.18854857865089 21.23824404275488 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 19.5 2 18.5 3 38.5 4 42.5 5 30.0 6 27.0 7 40.0 8 49.0 9 45.0 10 59.5 11 74.5 12 78.5 13 101.0 14 144.5 15 205.0 16 309.0 17 477.0 18 813.5 19 1395.0 20 2263.5 21 3610.5 22 5765.0 23 8937.5 24 13147.5 25 18561.5 26 25603.5 27 34263.5 28 44533.5 29 56755.0 30 70608.5 31 86258.0 32 105341.5 33 127242.0 34 150981.0 35 178649.5 36 211200.0 37 246584.0 38 279487.0 39 304142.5 40 321655.0 41 327889.0 42 319528.5 43 304798.5 44 286633.5 45 268601.0 46 253516.0 47 235822.5 48 214881.5 49 194178.0 50 174268.0 51 154388.0 52 133915.0 53 113133.0 54 93764.5 55 76893.5 56 62775.0 57 51155.5 58 41251.5 59 32755.5 60 25466.0 61 19472.5 62 14555.5 63 10670.0 64 7743.0 65 5621.5 66 4051.5 67 2887.5 68 2080.5 69 1499.5 70 1087.0 71 777.5 72 527.5 73 381.0 74 298.5 75 230.5 76 169.0 77 130.0 78 101.5 79 74.0 80 57.5 81 43.0 82 32.0 83 21.5 84 17.0 85 15.0 86 11.0 87 9.0 88 6.0 89 4.5 90 6.0 91 3.0 92 2.0 93 1.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17290683561493905 2 0.00643106766877457 3 0.0016382665606092398 4 0.0 5 9.062751186348987E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.4399714732478042E-4 12-13 0.0 14-15 8.714183833027873E-6 16-17 0.0 18-19 3.485673533211149E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.714183833027873E-6 38-39 2.4399714732478042E-4 40-41 6.0999286831195104E-5 42-43 0.0015162679869468497 44-45 6.884205228092019E-4 46-47 0.005934359190291981 48-49 0.004400662835679075 50-51 0.008330759744374645 52-53 0.004252521710517602 54-55 0.02022562067645769 56-57 0.01146786592426468 58-59 0.02034761925012008 60-61 0.009925455385818746 62-63 0.006370068381943374 64-65 0.013201988507037226 66-67 0.031310062512069144 68-69 0.03290475815351324 70-71 0.037462276298186825 72-73 0.0320769106893756 74-75 0.035135589214768385 76-77 0.03469116583928396 78-79 0.017925076144538334 80-81 0.0027101111720716684 82-83 0.0 84-85 0.0063874967496094295 86-87 0.026935542227889152 88-89 0.028181670516012138 90-91 0.025820126697261584 92-93 0.035771724634579415 94-95 0.032268622733702214 96-97 0.033845890007480256 98-99 0.04062552502957594 100-101 0.027615248566865327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5737772.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.03678752014928 #Duplication Level Percentage of deduplicated Percentage of total 1 96.68205459776654 84.14895443037277 2 2.2434706629970416 3.905289588059238 3 0.3822425612435904 0.9980749375234808 4 0.19702084079914936 0.6859224423070689 5 0.12452450840420276 0.5419106589513819 6 0.06923046955187695 0.3615358600984127 7 0.057403992740679054 0.3497381383285072 8 0.029465639500943788 0.20516756835111694 9 0.03391968618552876 0.26570344673520085 >10 0.15523960470419676 2.5016670529554657 >50 0.016693506009580986 1.0510344959668314 >100 0.008499925181350423 1.1981164134694584 >500 7.089631786988027E-5 0.0354864718127009 >1k 1.4308467262685024E-4 0.1720509740240879 >5k 0.0 0.0 >10k+ 2.0023924890464852E-5 3.579347521044137 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 205377 3.5793858661515308 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014814112516147383 0.0 1.7428367666055746E-5 2 0.00163826656060924 0.0 1.7428367666055746E-5 3 0.0020216906492624663 0.0 1.7428367666055746E-5 4 0.002248259428921191 0.0 1.7428367666055746E-5 5 0.0024748282085799156 0.0 1.7428367666055746E-5 6 0.0030499643415597554 0.0 3.485673533211149E-5 7 0.0038690976218643753 0.0 3.485673533211149E-5 8 0.004583660696172661 0.0 3.485673533211149E-5 9 0.005228510299816723 0.0 3.485673533211149E-5 10-11 0.007128202375416799 0.0 3.485673533211149E-5 12-13 0.008661898730029705 0.0 6.971347066422297E-5 14-15 0.010535448254130698 0.0 1.1328438982936233E-4 16-17 0.013045133198042725 0.0 1.481411251614738E-4 18-19 0.0156942450832832 0.0 1.9171204432661318E-4 20-21 0.01834335696852367 0.0 2.0914041199266892E-4 22-23 0.021271322736421037 0.0 2.2656877965872467E-4 24-25 0.02446942820314227 0.0 2.2656877965872467E-4 26-27 0.02779824642735891 0.0 2.2656877965872467E-4 28-29 0.031824199358217786 0.0 2.2656877965872467E-4 30-31 0.048111008942146885 0.0 2.4399714732478042E-4 32-33 0.05345280363179297 0.0 2.701396988238641E-4 34-35 0.057827323915972964 0.0 3.049964341559755E-4 36-37 0.06307326258345573 0.0 3.311389856550591E-4 38-39 0.06902505014141377 0.0 3.9213827248625426E-4 40-41 0.07527311994969477 0.0 4.1828082398533785E-4 42-43 0.0825756060017721 0.0 4.7928011081653293E-4 44-45 0.08977352184785314 0.0 5.141368461486444E-4 46-47 0.09778185679040576 0.0 5.664219491468117E-4 48-49 0.1066441817485951 0.0 6.09992868311951E-4 50-51 0.11601192936910007 0.0 6.79706338976174E-4 52-53 0.12526639259977568 1.7428367666055746E-5 7.319914419743412E-4 54-55 0.13575826993474122 1.7428367666055746E-5 7.668481773064527E-4 56-57 0.14667714227752515 1.7428367666055746E-5 7.668481773064527E-4 58-59 0.1601405562995532 1.7428367666055746E-5 7.842765449725085E-4 60-61 0.1736736837922455 1.7428367666055746E-5 7.929907288055364E-4 62-63 0.18876665019104977 4.3570919165139365E-5 8.627041994697593E-4 64-65 0.20476589170848894 5.228510299816723E-5 8.714183833027872E-4 66-67 0.22264739693386212 5.228510299816723E-5 8.714183833027872E-4 68-69 0.24191445738868678 5.228510299816723E-5 8.88846750968843E-4 70-71 0.2637609162580876 5.228510299816723E-5 8.88846750968843E-4 72-73 0.28680121831261335 5.228510299816723E-5 8.88846750968843E-4 74-75 0.312638773377541 5.228510299816723E-5 8.88846750968843E-4 76-77 0.3428769912781477 5.228510299816723E-5 8.88846750968843E-4 78-79 0.3762697437263105 5.228510299816723E-5 9.062751186348987E-4 80-81 0.4129477434795248 6.971347066422298E-5 9.062751186348987E-4 82-83 0.4543226883187411 6.971347066422298E-5 9.237034863009545E-4 84-85 0.5006124328397852 8.714183833027872E-5 9.237034863009545E-4 86-87 0.551790834491158 8.714183833027872E-5 9.237034863009545E-4 88-89 0.6112912817030722 8.714183833027872E-5 9.324176701339823E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28150 0.0 78.29652 3 CGGAAGA 28435 0.0 77.5278 4 GATCGGA 28565 0.0 77.464325 1 ATCGGAA 28655 0.0 76.97243 2 GAAGAGC 30780 0.0 71.76013 6 AGAGCAC 32330 0.0 68.29035 8 GAGCACA 32375 0.0 68.28345 9 AAGAGCA 35020 0.0 63.071873 7 GGAAGAG 37860 0.0 58.541855 5 GCCGTCT 24320 0.0 45.011814 50-51 TCTCGTA 24355 0.0 44.863834 42-43 TATGCCG 24380 0.0 44.63703 48-49 ATGCCGT 24510 0.0 44.623154 48-49 TGCCGTC 24370 0.0 44.61734 50-51 CGTATGC 24500 0.0 44.513794 46-47 CTCGTAT 24450 0.0 44.43814 44-45 CGTCTTC 24670 0.0 44.402687 52-53 TATCTCG 24360 0.0 44.394108 40-41 TCGTATG 24800 0.0 44.38556 44-45 ATCTCGT 24460 0.0 44.292595 42-43 >>END_MODULE