FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_35008-2.341000000358b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_35008-2.341000000358b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737772
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2053773.5793858661515308TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG281500.078.296523
CGGAAGA284350.077.52784
GATCGGA285650.077.4643251
ATCGGAA286550.076.972432
GAAGAGC307800.071.760136
AGAGCAC323300.068.290358
GAGCACA323750.068.283459
AAGAGCA350200.063.0718737
GGAAGAG378600.058.5418555
GCCGTCT243200.045.01181450-51
TCTCGTA243550.044.86383442-43
TATGCCG243800.044.6370348-49
ATGCCGT245100.044.62315448-49
TGCCGTC243700.044.6173450-51
CGTATGC245000.044.51379446-47
CTCGTAT244500.044.4381444-45
CGTCTTC246700.044.40268752-53
TATCTCG243600.044.39410840-41
TCGTATG248000.044.3855644-45
ATCTCGT244600.044.29259542-43
Produced by FastQC (version 0.11.2)