##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_35008-1.341000000357b4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2385580 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.683360021462285 33.0 33.0 33.0 33.0 33.0 2 32.71228129008459 33.0 33.0 33.0 33.0 33.0 3 32.74276402384326 33.0 33.0 33.0 33.0 33.0 4 36.68194191768878 37.0 37.0 37.0 37.0 37.0 5 36.65022929434351 37.0 37.0 37.0 37.0 37.0 6 36.521095079603285 37.0 37.0 37.0 37.0 37.0 7 36.46994273929191 37.0 37.0 37.0 37.0 37.0 8 36.397717117011375 37.0 37.0 37.0 37.0 37.0 9 36.42110304412344 37.0 37.0 37.0 37.0 37.0 10-11 36.36584876633775 37.0 37.0 37.0 37.0 37.0 12-13 36.34006090761995 37.0 37.0 37.0 37.0 37.0 14-15 38.153137811349865 40.0 37.0 40.0 37.0 40.0 16-17 38.131410809949784 40.0 37.0 40.0 37.0 40.0 18-19 38.15012910906363 40.0 37.0 40.0 37.0 40.0 20-21 38.089393774260344 40.0 37.0 40.0 37.0 40.0 22-23 38.002282882988624 40.0 37.0 40.0 33.0 40.0 24-25 37.968229319494625 40.0 37.0 40.0 33.0 40.0 26-27 37.85732945447229 40.0 37.0 40.0 33.0 40.0 28-29 37.75042777856957 40.0 37.0 40.0 33.0 40.0 30-31 37.696364406140226 40.0 37.0 40.0 33.0 40.0 32-33 37.5826367172763 40.0 37.0 40.0 33.0 40.0 34-35 37.49629880364523 40.0 37.0 40.0 33.0 40.0 36-37 37.4256759362503 40.0 37.0 40.0 33.0 40.0 38-39 37.324377929057086 40.0 37.0 40.0 33.0 40.0 40-41 37.232091147645434 40.0 37.0 40.0 33.0 40.0 42-43 37.109231088456475 38.5 37.0 40.0 33.0 40.0 44-45 37.008995296741254 37.0 37.0 40.0 33.0 40.0 46-47 36.92224993502629 37.0 37.0 40.0 33.0 40.0 48-49 36.70342432448294 37.0 37.0 40.0 33.0 40.0 50-51 36.51878557834992 37.0 37.0 40.0 33.0 40.0 52-53 36.394567778066545 37.0 37.0 40.0 33.0 40.0 54-55 36.23193164764963 37.0 37.0 40.0 33.0 40.0 56-57 36.05120180417341 37.0 37.0 40.0 33.0 40.0 58-59 35.79379459083326 37.0 37.0 40.0 27.0 40.0 60-61 35.53076777974329 37.0 33.0 40.0 27.0 40.0 62-63 35.30020707752412 37.0 33.0 40.0 27.0 40.0 64-65 34.98842042605991 37.0 33.0 37.0 27.0 40.0 66-67 34.62275232857418 37.0 33.0 37.0 27.0 40.0 68-69 34.33795722633489 37.0 33.0 37.0 27.0 40.0 70-71 34.01695520586189 37.0 33.0 37.0 27.0 40.0 72-73 33.604574568867946 37.0 33.0 37.0 27.0 37.0 74-75 33.13602813571542 37.0 33.0 37.0 27.0 37.0 76-77 30.493224708456644 33.0 30.0 35.0 24.5 37.0 78-79 32.90871884405469 35.0 33.0 37.0 27.0 37.0 80-81 33.32844046311588 37.0 33.0 37.0 27.0 37.0 82-83 33.28568880523814 37.0 33.0 37.0 27.0 37.0 84-85 33.162904618583326 37.0 33.0 37.0 27.0 37.0 86-87 33.00754198140494 37.0 33.0 37.0 27.0 37.0 88-89 32.876953612957855 35.0 33.0 37.0 27.0 37.0 90-91 32.729703677931575 33.0 33.0 37.0 27.0 37.0 92-93 32.54585048499736 33.0 33.0 37.0 27.0 37.0 94-95 32.45544416871368 33.0 33.0 37.0 27.0 37.0 96-97 32.320076878578796 33.0 33.0 37.0 22.0 37.0 98-99 32.02527247042648 33.0 33.0 37.0 22.0 37.0 100-101 30.436741798640163 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18458516469174668 2306 2 0.18382427955978642 2306 3 0.18947801199146852 2306 4 0.1958433890279423 2306 5 0.19395173620181794 2306 6 0.2484048171395017 2306 7 0.2820218405396986 2306 8 0.28030902427519777 2306 9 0.2718923874541588 2306 10-11 0.29244232874747667 2306 12-13 0.3124387802837916 2306 14-15 0.5862331855821807 2306 16-17 0.5684249453796539 2306 18-19 0.657872775210258 2306 20-21 0.6297382465789312 2306 22-23 0.6119537443970984 2306 24-25 0.628776361618506 2306 26-27 0.5762529788549671 2306 28-29 0.5825886768674309 2306 30-31 0.6629253904543191 2306 32-33 0.6864790077255307 2306 34-35 0.7215009842528346 2306 36-37 0.6103819291855643 2306 38-39 0.6599791725718447 2306 40-41 0.6525531188746996 2306 42-43 0.6930997728953088 2306 44-45 0.7354280307789409 2306 46-47 0.6990540607673594 2306 48-49 0.7996576203049841 2306 50-51 0.7835964888048181 2306 52-53 0.8094523105456588 2306 54-55 0.6942400904528725 2306 56-57 0.8397183777689037 2306 58-59 0.782498259500926 2306 60-61 0.7284979958101019 2306 62-63 0.7489818198835891 2306 64-65 0.7761132567448001 2306 66-67 0.8161654658060726 2306 68-69 0.7797966073725533 2306 70-71 0.6929486630316433 2306 72-73 0.7128911313828183 2306 74-75 0.5759124157331001 2306 76-77 0.052049719809755146 2306 78-79 0.6035604213532082 2306 80-81 0.8405114678102734 2306 82-83 0.702690763763762 2306 84-85 0.7008079159676157 2306 86-87 0.6432610175238267 2306 88-89 0.8621055947623759 2306 90-91 0.9442514856999509 2306 92-93 0.7811114136423996 2306 94-95 0.9387606049508008 2306 96-97 0.8455021020329099 2306 98-99 1.0406125089613028 2306 100-101 0.8329861892967259 2307 1 0.11065502930299687 2307 2 0.06702078246759413 2307 3 0.08168910413768771 2307 4 0.10296809650946415 2307 5 0.1866439874549215 2307 6 0.2298767160574755 2307 7 0.25225021734210884 2307 8 0.26926785157537125 2307 9 0.25430961341358227 2307 10-11 0.2102390370940057 2307 12-13 0.24969347023351673 2307 14-15 0.5446434297144336 2307 16-17 0.38414486907383605 2307 18-19 0.3935387905486536 2307 20-21 0.4083224185295862 2307 22-23 0.520372097952503 2307 24-25 0.5178456315387194 2307 26-27 0.5478661362009447 2307 28-29 0.652603891689914 2307 30-31 0.6025001477508667 2307 32-33 0.6636320595717748 2307 34-35 0.5237785736688707 2307 36-37 0.6180287290527247 2307 38-39 0.6284296548894446 2307 40-41 0.5847537073871152 2307 42-43 0.5635706600661976 2307 44-45 0.6643663142071361 2307 46-47 0.5887798077997033 2307 48-49 0.6998561080906427 2307 50-51 0.5470523387882054 2307 52-53 0.6061591124208618 2307 54-55 0.6533964015149039 2307 56-57 0.6229697158237215 2307 58-59 0.5390379300266517 2307 60-61 0.6314251114041269 2307 62-63 0.6142333278501368 2307 64-65 0.5180459253628769 2307 66-67 0.6483482908201736 2307 68-69 0.5931671153313687 2307 70-71 0.2612106978017579 2307 72-73 0.33206026305668246 2307 74-75 0.4519255143823031 2307 76-77 0.1313730077560642 2307 78-79 0.3216753280176121 2307 80-81 0.4393568404189594 2307 82-83 0.30782595943255586 2307 84-85 0.26443985628225164 2307 86-87 0.43155950608181115 2307 88-89 0.4458728465947175 2307 90-91 0.5875496267112368 2307 92-93 0.668853055144119 2307 94-95 0.7852955642878356 2307 96-97 0.6394470660811322 2307 98-99 0.587793132846258 2307 100-101 0.3593070598626049 2201 1 0.1249589369590467 2201 2 0.11391321486522799 2201 3 0.1396117571229354 2201 4 0.17021163296477937 2201 5 0.14328330808648104 2201 6 0.16181979599914342 2201 7 0.23153580567372245 2201 8 0.2589499714109067 2201 9 0.25735210472974757 2201 10-11 0.28947732266274784 2201 12-13 0.21350055893039865 2201 14-15 0.3687359304071762 2201 16-17 0.5180853011803777 2201 18-19 0.3984988630982187 2201 20-21 0.40453777765272747 2201 22-23 0.4728595515244223 2201 24-25 0.33996513119304694 2201 26-27 0.4959002725616557 2201 28-29 0.5993188687522704 2201 30-31 0.6076385153297181 2201 32-33 0.6101504082855058 2201 34-35 0.5919022494014357 2201 36-37 0.626156267377759 2201 38-39 0.637271213025663 2201 40-41 0.580439499484477 2201 42-43 0.6463965383161607 2201 44-45 0.7955794201513342 2201 46-47 0.7676034244737835 2201 48-49 0.7709034584361802 2201 50-51 0.6844584679947303 2201 52-53 0.7639236230332003 2201 54-55 0.7428359897289596 2201 56-57 0.6952859837756762 2201 58-59 0.79496393059204 2201 60-61 0.7766184229613984 2201 62-63 0.7714920627671304 2201 64-65 0.7978733962324966 2201 66-67 0.7121748324284098 2201 68-69 0.748555291879839 2201 70-71 0.7045828529884091 2201 72-73 0.5656294104593655 2201 74-75 0.655367140253837 2201 76-77 0.4316810355107492 2201 78-79 0.7558009126545642 2201 80-81 0.8415585181275986 2201 82-83 0.733520876204885 2201 84-85 0.45819365239894694 2201 86-87 0.6119368365825792 2201 88-89 0.7137282400893241 2201 90-91 0.7554171957690698 2201 92-93 0.677879066065735 2201 94-95 0.7453208110879004 2201 96-97 0.8357361783286308 2201 98-99 0.7522977273720173 2201 100-101 0.6199132349723939 1101 1 0.17118314176072147 1101 2 0.14174686671880465 1101 3 0.14053919076201993 1101 4 0.1810147144770724 1101 5 0.15572916344636667 1101 6 0.1198260569363967 1101 7 0.12949149867190357 1101 8 0.26035100435912994 1101 9 0.22502572106716912 1101 10-11 0.2186898098725223 1101 12-13 0.1668274610435745 1101 14-15 0.3759607904939273 1101 16-17 0.4677895106648293 1101 18-19 0.3591407079917772 1101 20-21 0.4188867724514935 1101 22-23 0.2922513527069768 1101 24-25 0.31709857064910807 1101 26-27 0.4266992794474689 1101 28-29 0.31901877555279157 1101 30-31 0.4803307639609997 1101 32-33 0.4110725966031836 1101 34-35 0.5274079820055988 1101 36-37 0.4506595962563722 1101 38-39 0.42033498245045564 1101 40-41 0.47464174691464933 1101 42-43 0.41397444654280946 1101 44-45 0.48947158546561553 1101 46-47 0.4340074449746325 1101 48-49 0.4695095712881283 1101 50-51 0.4399611142213615 1101 52-53 0.5510464350119904 1101 54-55 0.6330502725110776 1101 56-57 0.5513342194472131 1101 58-59 0.6006084285539686 1101 60-61 0.3359502298301891 1101 62-63 0.4960807611520295 1101 64-65 0.3143832353911762 1101 66-67 0.6166248414746818 1101 68-69 0.49985027811277405 1101 70-71 0.5244022618939042 1101 72-73 0.5995092454674023 1101 74-75 0.615294405130399 1101 76-77 0.36383812452461584 1101 78-79 0.6631742381427301 1101 80-81 0.5328531090609516 1101 82-83 0.6179968533930591 1101 84-85 0.4976415101032643 1101 86-87 0.48250092908875786 1101 88-89 0.6105018352934266 1101 90-91 0.5783332447801328 1101 92-93 0.6814941567793866 1101 94-95 0.6873341630915419 1101 96-97 0.4964246989807606 1101 98-99 0.6154476721060931 1101 100-101 0.6806141467460876 2304 1 0.02821030933256452 2304 2 0.06835726571047473 2304 3 0.12142261472700966 2304 4 0.12246440528804925 2304 5 0.15138610647962736 2304 6 0.11159921037211973 2304 7 0.17312349738933364 2304 8 0.1807867579698339 2304 9 0.14567683476199278 2304 10-11 0.13216689777483737 2304 12-13 0.21432383726188675 2304 14-15 0.3470814142936618 2304 16-17 0.3923493071164401 2304 18-19 0.35910176960965856 2304 20-21 0.45649736716277545 2304 22-23 0.3901112718201958 2304 24-25 0.2928619970257671 2304 26-27 0.25310763276955583 2304 28-29 0.37648097962965466 2304 30-31 0.38411984536460153 2304 32-33 0.4353266411135266 2304 34-35 0.40633176623169476 2304 36-37 0.40737621595717854 2304 38-39 0.44484092746214543 2304 40-41 0.49960284849861125 2304 42-43 0.5203112857007923 2304 44-45 0.5583010565910413 2304 46-47 0.493472634860332 2304 48-49 0.5411275330042713 2304 50-51 0.44076055003732506 2304 52-53 0.48874068444099095 2304 54-55 0.47159621055268985 2304 56-57 0.5664127368106477 2304 58-59 0.632387172673532 2304 60-61 0.5004275545600763 2304 62-63 0.38540623190107937 2304 64-65 0.44992854288221196 2304 66-67 0.41410677280930486 2304 68-69 0.49263320314119596 2304 70-71 0.5712122492120955 2304 72-73 0.3196424215855913 2304 74-75 0.3855507567113392 2304 76-77 0.3482454149773133 2304 78-79 0.4518202398331752 2304 80-81 0.20901617341341705 2304 82-83 0.4654810342471123 2304 84-85 0.48959679468117656 2304 86-87 0.41712041659570787 2304 88-89 0.5031325009707714 2304 90-91 0.3681110858946397 2304 92-93 0.411758506144146 2304 94-95 0.5420292527217541 2304 96-97 0.5031786241637946 2304 98-99 0.5146664608232356 2304 100-101 0.4465331508004766 2202 1 0.08821829760501032 2202 2 0.07184169446868083 2202 3 0.07927494733468876 2202 4 0.10653690367914948 2202 5 0.08767096013682618 2202 6 0.16305996129114675 2202 7 0.11684772423485867 2202 8 0.08903877190326881 2202 9 0.13712617080377498 2202 10-11 0.08216845045513566 2202 12-13 0.16892839100550106 2202 14-15 0.19740206954616468 2202 16-17 0.24286056726106153 2202 18-19 0.24329237878503562 2202 20-21 0.20323689915280596 2202 22-23 0.19111355372483274 2202 24-25 0.2744944315535207 2202 26-27 0.2555983461351303 2202 28-29 0.1942650585529293 2202 30-31 0.27489092781834756 2202 32-33 0.3052321631942405 2202 34-35 0.44662671124003595 2202 36-37 0.4008804440662601 2202 38-39 0.2654265770554005 2202 40-41 0.29256610137455397 2202 42-43 0.3143327169722099 2202 44-45 0.28610174383946685 2202 46-47 0.24196712391607633 2202 48-49 0.3282492813437159 2202 50-51 0.3231124644921337 2202 52-53 0.2051339901401832 2202 54-55 0.23242765479165683 2202 56-57 0.3112172008200531 2202 58-59 0.4019602070070363 2202 60-61 0.35926193068777934 2202 62-63 0.4270899155869117 2202 64-65 0.25169238770413216 2202 66-67 0.19433322855416435 2202 68-69 0.1341257465279 2202 70-71 0.189518135797897 2202 72-73 0.2036165121700293 2202 74-75 0.13495918551666364 2202 76-77 0.015014844094245916 2202 78-79 0.283840790895475 2202 80-81 0.33750582238988613 2202 82-83 0.31715444882667754 2202 84-85 0.3608303402855384 2202 86-87 0.2817849466315039 2202 88-89 0.37974999228914186 2202 90-91 0.4491815572348372 2202 92-93 0.3268976744857426 2202 94-95 0.39633012361478137 2202 96-97 0.4647568540868292 2202 98-99 0.4028917900717701 2202 100-101 0.4017737680605009 1102 1 0.10741456991419795 1102 2 0.0502472285437463 1102 3 0.06323681255592106 1102 4 0.06382162164405258 1102 5 0.10933077800117985 1102 6 0.13981186873823503 1102 7 0.06957095775979383 1102 8 0.10928229386698263 1102 9 0.07728787133002868 1102 10-11 0.10796425700530676 1102 12-13 0.14584064338134084 1102 14-15 0.13216586173913925 1102 16-17 0.14400149477301483 1102 18-19 0.1557747003772718 1102 20-21 0.21215915867082202 1102 22-23 0.2423230704239714 1102 24-25 0.29033711749799807 1102 26-27 0.22756125132221428 1102 28-29 0.19884758510341527 1102 30-31 0.15946938757632267 1102 32-33 -0.0072394350147462205 1102 34-35 0.17477559226511374 1102 36-37 0.21728671461519866 1102 38-39 0.27971194449313685 1102 40-41 0.2812404888771525 1102 42-43 0.1639443484034544 1102 44-45 0.1605157514105784 1102 46-47 0.2347189261526097 1102 48-49 0.17179754769636446 1102 50-51 0.21659376028148358 1102 52-53 0.1548494158281457 1102 54-55 0.308388763306219 1102 56-57 0.24346585382148334 1102 58-59 0.22113270577789734 1102 60-61 0.08412136331963893 1102 62-63 0.2742891904434046 1102 64-65 0.25148459686270996 1102 66-67 0.1860531580630962 1102 68-69 0.290835853013121 1102 70-71 0.24687994072539965 1102 72-73 0.31285844990561884 1102 74-75 0.2667922568352594 1102 76-77 0.14662535462845128 1102 78-79 0.21960145307450318 1102 80-81 0.1962070385834096 1102 82-83 0.30371693681345135 1102 84-85 0.2458013163359709 1102 86-87 0.2799676366630024 1102 88-89 0.2566567818093617 1102 90-91 0.3640988961362268 1102 92-93 0.35344806325896627 1102 94-95 0.25824117213910114 1102 96-97 0.2276900635954462 1102 98-99 0.2719981275912673 1102 100-101 0.20375476136951676 2305 1 0.04025227124360953 2305 2 0.013336930512103606 2305 3 -0.0027510879937082677 2305 4 0.07358689950620345 2305 5 0.06887366265593187 2305 6 0.08348238032135669 2305 7 0.014022151618725331 2305 8 0.041396682943727114 2305 9 -0.052822841565628664 2305 10-11 0.03282865001921209 2305 12-13 -0.005051516723774796 2305 14-15 0.0270060468530815 2305 16-17 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-0.4240119301681631 1213 62-63 -0.12465836677613851 1213 64-65 -0.5210754486535549 1213 66-67 -0.4592306379374733 1213 68-69 -0.03677035719672972 1213 70-71 0.11410694181524406 1213 72-73 0.11361699165602346 1213 74-75 0.2121701892337171 1213 76-77 -2.2421721992214074 1213 78-79 -1.8711664674105748 1213 80-81 -1.3067694275856212 1213 82-83 -0.8406493246585782 1213 84-85 -0.46694002900901665 1213 86-87 -0.2948345520485205 1213 88-89 -0.4104780832139241 1213 90-91 -1.5319505300371752 1213 92-93 -0.6130452458341082 1213 94-95 -0.34946787658117984 1213 96-97 -0.3797422949992608 1213 98-99 -2.136697352975581 1213 100-101 -0.7558401567686062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 8.0 9 27.0 10 31.0 11 21.0 12 26.0 13 64.0 14 195.0 15 452.0 16 820.0 17 1434.0 18 2217.0 19 2976.0 20 4230.0 21 5601.0 22 7081.0 23 9163.0 24 11992.0 25 14959.0 26 19352.0 27 25795.0 28 35378.0 29 47612.0 30 51213.0 31 58315.0 32 78355.0 33 111007.0 34 169430.0 35 289877.0 36 539836.0 37 741207.0 38 156853.0 39 52.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.70099617553248 25.486204539308705 24.710603533559407 15.102195751599407 2 28.67411129379392 23.40869627916765 34.02718331339287 13.890009113645569 3 36.855715803792435 19.43396345054673 26.886002510967145 16.824318234693685 4 27.767251569848845 19.29987675953018 34.744758088179815 18.18811358244117 5 32.2542012165702 18.193010527092394 32.68680422492171 16.8659840314157 6 25.73906555219276 19.40375086980944 38.089982310381544 16.76720126761626 7 31.440697859640004 20.856730857904576 29.679449022879133 18.023122259576287 8 27.447497044743834 28.225924093931038 26.625684319955734 17.700894541369394 9 30.406442039252507 24.521541931102707 25.96295240570427 19.109063623940507 10-11 24.260026874007185 28.756298529936053 29.04341990290397 17.940254693152788 12-13 23.715385776205366 26.875854089990693 28.976915467098145 20.43184466670579 14-15 22.955444797491594 27.43588561272311 29.795877732040005 19.812791857745285 16-17 22.658074765885026 27.586373125193873 30.598198341703064 19.157353767218037 18-19 20.905633201492552 27.888674429001426 31.488323906203924 19.717368463302105 20-21 21.86097301285222 28.085937172511503 30.975968108384542 19.077121706251727 22-23 21.117401218990768 29.520598764241818 30.272156037525466 19.089843979241948 24-25 21.51246237812188 27.344880490279095 31.191869482473866 19.95078764912516 26-27 21.3923029200446 27.545754072384916 30.218961426571315 20.84298158099917 28-29 21.421499174205017 28.70249582910655 30.05042798816221 19.82557700852623 30-31 20.17459066558237 28.6959146203439 30.495833298401227 20.6336614156725 32-33 19.97717536196648 29.520284375288192 30.34855255325749 20.15398770948784 34-35 21.07355443959121 28.85786684999036 30.631712204159996 19.436866506258436 36-37 20.14946050855557 29.326557902061552 31.303875787020345 19.22010580236253 38-39 20.716233731392332 29.74128680033669 29.70037424923792 19.842105219033055 40-41 20.654482622457692 28.54443302862743 31.11313857139085 19.68794577752403 42-43 20.64513857289484 29.467277874552593 30.754004475698366 19.1335790768542 44-45 20.101007433452928 28.857877393508545 31.21027411931838 19.830841053720142 46-47 21.088705434639987 28.75124137639035 30.3157843549475 19.84426883402216 48-49 20.665906653344425 30.089673607185457 30.087263195380228 19.15715654408989 50-51 21.399401314207754 29.353462523866625 29.659324266058633 19.587811895866988 52-53 20.229688996353573 28.913326484974867 29.422740392787805 21.434244125883755 54-55 21.436362351874752 29.049308803000628 29.825984344901045 19.688344500223575 56-57 20.702868220174132 28.974763049865178 29.431894024764894 20.8904747051958 58-59 20.547630467667766 28.781352825359985 30.248118327357375 20.422898379614878 60-61 20.819450010815995 29.119049735133874 30.374816169997267 19.686684084052864 62-63 20.21055641062671 29.23739693469741 29.970405897608472 20.5816407570674 64-65 21.180873116374503 28.987316060447064 29.794837922314983 20.036972900863447 66-67 20.84986209867885 30.21090358648263 28.879691973210143 20.05954234162838 68-69 21.04776415242882 30.321914331593923 28.685537620059282 19.94478389591798 70-71 20.52790320027425 31.18254048959678 28.778838583605815 19.510717726523165 72-73 20.21210620573346 31.4125919400145 28.68012695175322 19.69517490249882 74-75 20.887209992754013 30.59639344372283 28.520174708818637 19.996221854704523 76-77 21.31029614243485 29.66626082934642 29.057861275760484 19.965581752458245 78-79 21.147388366714328 29.45054733122181 29.686715528425818 19.715348773638045 80-81 20.360491330618625 30.06977089291149 29.39415356471246 20.175584211757428 82-83 19.55052859262737 29.982436137123887 29.651405528215363 20.815629742033384 84-85 19.65172758052369 29.493699567562516 30.0996276774244 20.754945174489396 86-87 19.724981734283883 29.6677159626111 30.68990422160795 19.917398081497065 88-89 19.937029241904273 30.18359342979094 30.22260947081376 19.65676785749103 90-91 20.29524936823025 29.580052889620696 29.685127718748312 20.439570023400744 92-93 19.68203588947511 30.38662912839247 29.585963570429968 20.34537141170245 94-95 19.274841609169908 30.914615187698818 29.577316256888025 20.233226946243246 96-97 19.86385090313199 31.364212357200483 28.985219799296548 19.78671694037098 98-99 20.311549262935685 30.33822808505856 28.645934456986165 20.70428819501959 100-101 20.23222503991735 30.23022078653947 28.33991214829127 21.197642025251913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 135.0 1 85.5 2 63.0 3 143.5 4 157.0 5 96.0 6 68.0 7 70.5 8 75.0 9 66.0 10 59.0 11 60.5 12 71.0 13 76.5 14 78.5 15 88.5 16 105.0 17 128.0 18 155.0 19 206.5 20 285.0 21 449.0 22 703.0 23 1063.0 24 1585.5 25 2267.5 26 3325.5 27 4954.0 28 6689.5 29 8530.5 30 10801.5 31 13400.0 32 18811.0 33 29487.0 34 45679.5 35 69030.0 36 102235.5 37 143491.0 38 184691.5 39 202563.5 40 199362.5 41 180029.0 42 154256.5 43 144764.0 44 136647.0 45 126713.5 46 123880.0 47 109802.5 48 81952.0 49 62047.0 50 49622.5 51 40345.0 52 33312.5 53 26081.5 54 19250.0 55 13599.5 56 9421.5 57 6326.0 58 4410.0 59 3231.0 60 2419.0 61 1777.0 62 1225.0 63 866.0 64 642.0 65 460.5 66 319.0 67 222.0 68 164.0 69 118.0 70 85.5 71 65.5 72 48.5 73 37.0 74 35.0 75 25.5 76 13.0 77 6.5 78 7.0 79 6.5 80 4.0 81 4.5 82 3.0 83 1.5 84 2.0 85 2.5 86 1.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1706922425573655 2 0.006120104963991985 3 0.0018863337217783515 4 0.0 5 8.802890701632307E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.4671484502720513E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.095926357531502E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.6767410860252016E-4 40-41 4.191852715063004E-5 42-43 0.001278515078094216 44-45 5.449408529581905E-4 46-47 0.005155978839527495 48-49 0.004003219342885169 50-51 0.007901642367893761 52-53 0.003919382288583908 54-55 0.020058015241576473 56-57 0.011234165276368849 58-59 0.019617870706494855 60-61 0.009389750081741128 62-63 0.0058057160103622595 64-65 0.013392969424626295 66-67 0.0302861358663302 68-69 0.03221438811525918 70-71 0.03615472966741841 72-73 0.030893954510014334 74-75 0.034037844046311584 76-77 0.033471943929778085 78-79 0.01741714803108678 80-81 0.002431274574736542 82-83 0.0 84-85 0.00622490128186856 86-87 0.027142246330032947 88-89 0.02781294276444303 90-91 0.025696057143336216 92-93 0.03412168110061285 94-95 0.030831076719288395 96-97 0.032193428851683865 98-99 0.03843928939712774 100-101 0.02733087970221078 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2385580.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.72198586863029 #Duplication Level Percentage of deduplicated Percentage of total 1 87.04743862456262 34.57697126945338 2 5.60431446095346 4.452289996427075 3 2.071509972142251 2.468534695204837 4 1.1548909151136915 1.8349824243982225 5 0.7672677267110295 1.5238698898935799 6 0.41433233271043357 0.987486183890428 7 0.3632181405478797 1.0099422092251134 8 0.26700530278298024 0.8484784691195915 9 0.22350366530111657 0.7990208491210224 >10 1.6121281576561504 12.791971867242474 >50 0.23267729064695855 6.532279702130182 >100 0.20291728416646565 17.634212728317582 >500 0.030825150871765204 8.249083669568611 >1k 0.007759718107837789 4.6431061078385465 >5k 1.0562886274459934E-4 0.21092227247520348 >10k+ 1.0562886266461487E-4 1.4368476656940334 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 34245 1.4354999622733255 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5027 0.2107244359862172 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3855 0.16159592216567878 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3831 0.16058987751406367 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 3703 0.155224306038783 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3193 0.1338458571919617 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3060 0.1282706930809279 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2881 0.12076727672096514 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2719 0.11397647532256307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.545334887113407E-4 0.0 0.0 2 8.383705430126007E-4 0.0 0.0 3 9.222075973138608E-4 0.0 0.0 4 0.0010060446516151208 0.0 0.0 5 0.0011318002330670109 0.0 0.0 6 0.001215637287368271 0.0 0.0 7 0.0014671484502720513 0.0 0.0 8 0.0015929040317239414 0.0 0.0 9 0.001970170776079612 0.0 0.0 10-11 0.003584034071378868 0.0 2.0959263575315017E-5 12-13 0.004631997250144619 0.0 4.191852715063003E-5 14-15 0.00622490128186856 0.0 4.191852715063003E-5 16-17 0.007440538569236831 0.0 6.287779072594505E-5 18-19 0.009368790818165813 0.0 8.383705430126007E-5 20-21 0.01182102465647767 0.0 8.383705430126007E-5 22-23 0.014419973339816733 0.0 8.383705430126007E-5 24-25 0.01750098508538804 0.0 8.383705430126007E-5 26-27 0.020456241249507456 0.0 8.383705430126007E-5 28-29 0.024417542065241996 0.0 8.383705430126007E-5 30-31 0.03959204889377007 0.0 8.383705430126007E-5 32-33 0.044245005407490004 0.0 8.383705430126007E-5 34-35 0.0478709580060195 0.0 8.383705430126007E-5 36-37 0.05264967010119133 0.0 8.383705430126007E-5 38-39 0.057218789560609995 0.0 8.383705430126007E-5 40-41 0.06214421650080902 0.0 8.383705430126007E-5 42-43 0.06767746208469219 0.0 8.383705430126007E-5 44-45 0.07356701514935571 0.0 8.383705430126007E-5 46-47 0.08000150906697742 0.0 8.383705430126007E-5 48-49 0.08656175856605103 0.0 1.257555814518901E-4 50-51 0.09335255996445309 0.0 1.257555814518901E-4 52-53 0.09955650198274633 0.0 1.257555814518901E-4 54-55 0.10701799981555848 0.0 1.257555814518901E-4 56-57 0.11538074598210918 0.0 1.257555814518901E-4 58-59 0.12470761827312435 0.0 1.257555814518901E-4 60-61 0.1345584721535224 0.0 1.257555814518901E-4 62-63 0.14547824847626153 0.0 1.257555814518901E-4 64-65 0.1562932284811241 0.0 1.257555814518901E-4 66-67 0.16876399030843653 0.0 1.257555814518901E-4 68-69 0.18330971922970515 0.0 1.257555814518901E-4 70-71 0.1985890223761098 0.0 1.257555814518901E-4 72-73 0.2150839628098827 0.0 1.257555814518901E-4 74-75 0.23346523696543398 0.0 1.257555814518901E-4 76-77 0.2534813336798598 0.0 1.257555814518901E-4 78-79 0.2770395459385139 0.0 1.257555814518901E-4 80-81 0.30252601044609695 0.0 1.257555814518901E-4 82-83 0.33180610166081204 0.0 1.257555814518901E-4 84-85 0.3615892152013347 0.0 1.467148450272051E-4 86-87 0.3974295559151234 0.0 1.6767410860252013E-4 88-89 0.43511431182353977 0.0 1.6767410860252013E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 5305 0.0 69.20161 4 TCGGAAG 5350 0.0 68.7083 3 GATCGGA 5475 0.0 67.32231 1 ATCGGAA 5570 0.0 66.171974 2 GAAGAGC 5605 0.0 65.41295 6 CGACGCG 30 1.2934134E-4 63.413273 1 GGAAGAG 6350 0.0 58.18726 5 GTCCGGC 35 2.7893795E-4 54.282455 2 TTGTGTT 26335 0.0 48.363605 1 CGACGCC 40 5.3559395E-4 47.559956 1 CCGCCGA 50 2.8306733E-5 47.492172 3 GCCGTCT 4060 0.0 44.519554 50-51 ATGCCGT 4025 0.0 44.256176 48-49 CGTCTTC 4115 0.0 43.982235 52-53 TGCCGTC 4080 0.0 43.952034 50-51 GGCCGGT 65 2.5681657E-6 43.9015 1 TCGTATG 4095 0.0 43.782776 44-45 TCTCGTA 4030 0.0 43.722 42-43 CGTATGC 4055 0.0 43.68989 46-47 TATGCCG 4045 0.0 43.50891 48-49 >>END_MODULE