FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_35008-1.341000000357b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_35008-1.341000000357b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385580
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT342451.4354999622733255TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC50270.2107244359862172No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG38550.16159592216567878No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA38310.16058987751406367No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC37030.155224306038783No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA31930.1338458571919617No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA30600.1282706930809279No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG28810.12076727672096514No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC27190.11397647532256307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA53050.069.201614
TCGGAAG53500.068.70833
GATCGGA54750.067.322311
ATCGGAA55700.066.1719742
GAAGAGC56050.065.412956
CGACGCG301.2934134E-463.4132731
GGAAGAG63500.058.187265
GTCCGGC352.7893795E-454.2824552
TTGTGTT263350.048.3636051
CGACGCC405.3559395E-447.5599561
CCGCCGA502.8306733E-547.4921723
GCCGTCT40600.044.51955450-51
ATGCCGT40250.044.25617648-49
CGTCTTC41150.043.98223552-53
TGCCGTC40800.043.95203450-51
GGCCGGT652.5681657E-643.90151
TCGTATG40950.043.78277644-45
TCTCGTA40300.043.72242-43
CGTATGC40550.043.6898946-47
TATGCCG40450.043.5089148-49
Produced by FastQC (version 0.11.2)