##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_35005-1.3410000003578e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2608703 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70007317812721 33.0 33.0 33.0 33.0 33.0 2 32.72326247947735 33.0 33.0 33.0 33.0 33.0 3 32.75071826881021 33.0 33.0 33.0 33.0 33.0 4 36.69527539164098 37.0 37.0 37.0 37.0 37.0 5 36.65440987341219 37.0 37.0 37.0 37.0 37.0 6 36.539941879163706 37.0 37.0 37.0 37.0 37.0 7 36.49870567864567 37.0 37.0 37.0 37.0 37.0 8 36.42805294431754 37.0 37.0 37.0 37.0 37.0 9 36.441502539767846 37.0 37.0 37.0 37.0 37.0 10-11 36.38966739410351 37.0 37.0 37.0 37.0 37.0 12-13 36.37419917100567 37.0 37.0 37.0 37.0 37.0 14-15 38.20749947387648 40.0 37.0 40.0 37.0 40.0 16-17 38.19956200456702 40.0 37.0 40.0 37.0 40.0 18-19 38.20615301933566 40.0 37.0 40.0 37.0 40.0 20-21 38.12700909225772 40.0 37.0 40.0 37.0 40.0 22-23 38.041889398678194 40.0 37.0 40.0 37.0 40.0 24-25 38.00988019717077 40.0 37.0 40.0 35.0 40.0 26-27 37.91494106458266 40.0 37.0 40.0 33.0 40.0 28-29 37.80992661870669 40.0 37.0 40.0 33.0 40.0 30-31 37.74819536758305 40.0 37.0 40.0 33.0 40.0 32-33 37.626967692374336 40.0 37.0 40.0 33.0 40.0 34-35 37.551642137874644 40.0 37.0 40.0 33.0 40.0 36-37 37.48172750980085 40.0 37.0 40.0 33.0 40.0 38-39 37.38940116985337 40.0 37.0 40.0 33.0 40.0 40-41 37.29852670081646 40.0 37.0 40.0 33.0 40.0 42-43 37.17666499406027 40.0 37.0 40.0 33.0 40.0 44-45 37.068832481121845 40.0 37.0 40.0 33.0 40.0 46-47 36.963676393978155 37.0 37.0 40.0 33.0 40.0 48-49 36.75290690431222 37.0 37.0 40.0 33.0 40.0 50-51 36.58400323839088 37.0 37.0 40.0 33.0 40.0 52-53 36.46156404159461 37.0 37.0 40.0 33.0 40.0 54-55 36.288929594514975 37.0 37.0 40.0 33.0 40.0 56-57 36.092339564910226 37.0 37.0 40.0 33.0 40.0 58-59 35.85411332758079 37.0 37.0 40.0 27.0 40.0 60-61 35.59691559368774 37.0 35.0 40.0 27.0 40.0 62-63 35.34126594710092 37.0 33.0 40.0 27.0 40.0 64-65 35.03702452904757 37.0 33.0 37.0 27.0 40.0 66-67 34.70435883272262 37.0 33.0 37.0 27.0 40.0 68-69 34.40669060448813 37.0 33.0 37.0 27.0 40.0 70-71 34.11166219381815 37.0 33.0 37.0 27.0 40.0 72-73 33.75234283090103 37.0 33.0 37.0 27.0 37.0 74-75 33.34132248860833 37.0 33.0 37.0 27.0 37.0 76-77 30.63856483470905 33.0 30.0 35.0 24.5 37.0 78-79 33.09319937915508 35.0 33.0 37.0 27.0 37.0 80-81 33.521292190027 37.0 33.0 37.0 27.0 37.0 82-83 33.47824800293479 37.0 33.0 37.0 27.0 37.0 84-85 33.35681466997201 37.0 33.0 37.0 27.0 37.0 86-87 33.2012885330373 37.0 33.0 37.0 27.0 37.0 88-89 33.05953571564107 37.0 33.0 37.0 27.0 37.0 90-91 32.92267115114292 35.0 33.0 37.0 27.0 37.0 92-93 32.76632851650801 33.0 33.0 37.0 27.0 37.0 94-95 32.672276606420894 33.0 33.0 37.0 27.0 37.0 96-97 32.52746978096012 33.0 33.0 37.0 27.0 37.0 98-99 32.23515612931024 33.0 33.0 37.0 22.0 37.0 100-101 30.59777138294394 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17536600703285643 2306 2 0.15951816492142967 2306 3 0.13469760268172593 2306 4 0.16004349862271994 2306 5 0.2059286965064402 2306 6 0.2350800923931402 2306 7 0.24416101409055102 2306 8 0.26087733546257397 2306 9 0.25465817267836854 2306 10-11 0.24204685412973959 2306 12-13 0.2531192470165067 2306 14-15 0.5718604368675742 2306 16-17 0.5635248556913055 2306 18-19 0.5489528303427775 2306 20-21 0.560503428415636 2306 22-23 0.5595025632715291 2306 24-25 0.5505848041155517 2306 26-27 0.5383440579753866 2306 28-29 0.6137056242460588 2306 30-31 0.5969176563858767 2306 32-33 0.7986639544131435 2306 34-35 0.7411660865555731 2306 36-37 0.7079414064424725 2306 38-39 0.7417666032035584 2306 40-41 0.7642884776453869 2306 42-43 0.8840004646688442 2306 44-45 0.9667247516077566 2306 46-47 0.9274935810413112 2306 48-49 0.8944743237489376 2306 50-51 0.9871371061662018 2306 52-53 0.9802123987866764 2306 54-55 0.8967041195053298 2306 56-57 0.8651735425140288 2306 58-59 0.8012124728951449 2306 60-61 0.8166329131980277 2306 62-63 0.924153520441422 2306 64-65 0.9051764401158167 2306 66-67 0.9584128842269237 2306 68-69 1.0329071181508311 2306 70-71 0.7521085089326007 2306 72-73 0.6784575244448448 2306 74-75 0.859469883896665 2306 76-77 0.5282585731664149 2306 78-79 0.7047421212599332 2306 80-81 0.80673853633796 2306 82-83 0.9699327565395066 2306 84-85 0.934043856467575 2306 86-87 0.6706822085703052 2306 88-89 0.8799422819274199 2306 90-91 0.9992550705388155 2306 92-93 0.9100379148483881 2306 94-95 0.8879386730611714 2306 96-97 0.9596367496815716 2306 98-99 0.9033899274693624 2306 100-101 0.5930197661849874 2307 1 0.10941507553806673 2307 2 0.10884338129226734 2307 3 0.17111941579599943 2307 4 0.18198680212865526 2307 5 0.23230786413358828 2307 6 0.22245566606400757 2307 7 0.1965033724802865 2307 8 0.28474623464319393 2307 9 0.24584354957226395 2307 10-11 0.3042374909829846 2307 12-13 0.26383444955637003 2307 14-15 0.5643381449083975 2307 16-17 0.5754446360722838 2307 18-19 0.4524506509173918 2307 20-21 0.58332334815411 2307 22-23 0.5370202972978149 2307 24-25 0.5683604804708864 2307 26-27 0.5923205924794601 2307 28-29 0.6364425057817087 2307 30-31 0.6458685459686393 2307 32-33 0.7011425268895124 2307 34-35 0.6720208037541724 2307 36-37 0.7278687414662599 2307 38-39 0.6506371526702281 2307 40-41 0.6277318911703986 2307 42-43 0.6414720692707832 2307 44-45 0.6872739563270471 2307 46-47 0.6136838302083092 2307 48-49 0.7098433329486227 2307 50-51 0.6806036760859442 2307 52-53 0.623620553819265 2307 54-55 0.6732830241345127 2307 56-57 0.5752744719132252 2307 58-59 0.5805105512287199 2307 60-61 0.5719435599006033 2307 62-63 0.5443815742564624 2307 64-65 0.6314021049453444 2307 66-67 0.6041225334232649 2307 68-69 0.6027888738456255 2307 70-71 0.5107865250268233 2307 72-73 0.4700502338478074 2307 74-75 0.5673644070425823 2307 76-77 0.263626691735535 2307 78-79 0.5784175702425216 2307 80-81 0.6456012011046113 2307 82-83 0.7279483006623906 2307 84-85 0.7781532520961534 2307 86-87 0.7224967373743496 2307 88-89 0.6457717995199985 2307 90-91 0.6946075506829956 2307 92-93 0.7513249284069303 2307 94-95 0.7285266726001396 2307 96-97 0.7300676839006357 2307 98-99 0.7485253819005067 2307 100-101 0.7711008240445381 2201 1 0.13283592681895584 2201 2 0.10620934139201665 2201 3 0.12979564189741666 2201 4 0.1121101207261006 2201 5 0.18582747419933554 2201 6 0.16576429789352431 2201 7 0.1841637197182564 2201 8 0.27481131814655413 2201 9 0.2562377817947379 2201 10-11 0.2204774309059161 2201 12-13 0.18732673683061307 2201 14-15 0.4763637154517397 2201 16-17 0.48674073295129716 2201 18-19 0.5087663011856662 2201 20-21 0.5039398939109248 2201 22-23 0.4746831241322411 2201 24-25 0.4625385060508762 2201 26-27 0.4781358837966181 2201 28-29 0.37399894612025975 2201 30-31 0.44365379402274385 2201 32-33 0.46741753539098596 2201 34-35 0.46417347132766196 2201 36-37 0.46142689154560657 2201 38-39 0.5016341866005547 2201 40-41 0.43903223878620423 2201 42-43 0.4105693308516791 2201 44-45 0.519864473405562 2201 46-47 0.5710967609496365 2201 48-49 0.5808960515309565 2201 50-51 0.4060975880862472 2201 52-53 0.46289240260639275 2201 54-55 0.4792755003303881 2201 56-57 0.5660365581748366 2201 58-59 0.5443608686170691 2201 60-61 0.6129488827676752 2201 62-63 0.555758435134571 2201 64-65 0.5211762491303276 2201 66-67 0.5785924901602115 2201 68-69 0.5433635734602262 2201 70-71 0.46642406470036946 2201 72-73 0.4810978987763974 2201 74-75 0.5725161819613405 2201 76-77 0.20362104770168088 2201 78-79 0.4244888267603173 2201 80-81 0.504621382658307 2201 82-83 0.5011747988109647 2201 84-85 0.4821582726886149 2201 86-87 0.6245771188071316 2201 88-89 0.6180514460476232 2201 90-91 0.5948254009437122 2201 92-93 0.6371176672038743 2201 94-95 0.7049546840110068 2201 96-97 0.6933273850266133 2201 98-99 0.6555524402017312 2201 100-101 0.5302850131928807 1101 1 0.08850834824664133 1101 2 0.09163922358504095 1101 3 0.10210911418256785 1101 4 0.18269368919283124 1101 5 0.15231324262212098 1101 6 0.10370903468142956 1101 7 0.17045883271958218 1101 8 0.18818654641474097 1101 9 0.17309045270410905 1101 10-11 0.14443773798669213 1101 12-13 0.14050118778555998 1101 14-15 0.3164247385737653 1101 16-17 0.35609281173643126 1101 18-19 0.2884360160049013 1101 20-21 0.3315781077209792 1101 22-23 0.23610844010345744 1101 24-25 0.2650935006616848 1101 26-27 0.28694797960746854 1101 28-29 0.33425947220118957 1101 30-31 0.377134965330292 1101 32-33 0.3060987588741213 1101 34-35 0.35221779654632 1101 36-37 0.42922048218694897 1101 38-39 0.41093577177719 1101 40-41 0.37602936226384287 1101 42-43 0.49555085056855575 1101 44-45 0.4597485243248087 1101 46-47 0.34409274222820585 1101 48-49 0.455165121363855 1101 50-51 0.37871001874763977 1101 52-53 0.4610008231278755 1101 54-55 0.40582152382999936 1101 56-57 0.5542342993496163 1101 58-59 0.5619636922773239 1101 60-61 0.35636718592841987 1101 62-63 0.3016667708707246 1101 64-65 0.33845572147040315 1101 66-67 0.3430723948367884 1101 68-69 0.4236360535854544 1101 70-71 0.4820352270144852 1101 72-73 0.4598033092472136 1101 74-75 0.25321050769489517 1101 76-77 0.19012501321774167 1101 78-79 0.505447977911615 1101 80-81 0.42851421761182706 1101 82-83 0.44726102926227895 1101 84-85 0.3406220235295976 1101 86-87 0.29717264389260123 1101 88-89 0.5421373649901042 1101 90-91 0.4211151376286395 1101 92-93 0.4582215970208523 1101 94-95 0.48768349282867973 1101 96-97 0.4723880271295613 1101 98-99 0.4874360345846185 1101 100-101 0.5024704064320247 2304 1 0.08373037898251567 2304 2 0.10525410121734069 2304 3 0.11955182821473898 2304 4 0.17256276397077386 2304 5 0.18464644998337576 2304 6 0.1879469596640675 2304 7 0.20294142581855823 2304 8 0.20117677401528766 2304 9 0.17529503004139713 2304 10-11 0.1606434193934092 2304 12-13 0.22482280835370716 2304 14-15 0.348386757002622 2304 16-17 0.3412522785381711 2304 18-19 0.3695873942119974 2304 20-21 0.3630911774890677 2304 22-23 0.4119682984739157 2304 24-25 0.4140187147977272 2304 26-27 0.5113608738720501 2304 28-29 0.5273108589163442 2304 30-31 0.525758341131386 2304 32-33 0.3844598885073012 2304 34-35 0.4486605363005367 2304 36-37 0.43128596260554986 2304 38-39 0.536235384165721 2304 40-41 0.4570238359808627 2304 42-43 0.523286637653321 2304 44-45 0.4688288028352332 2304 46-47 0.4673384491195449 2304 48-49 0.6741601609659185 2304 50-51 0.5770808238279415 2304 52-53 0.488759562193529 2304 54-55 0.6511734509060076 2304 56-57 0.6343244452331973 2304 58-59 0.7371680341589482 2304 60-61 0.6958994997453161 2304 62-63 0.5502337560291011 2304 64-65 0.39048202523195386 2304 66-67 0.4830943179357803 2304 68-69 0.5601460268147349 2304 70-71 0.5410128151965949 2304 72-73 0.654398315608475 2304 74-75 0.5734104273629583 2304 76-77 0.39901583895482773 2304 78-79 0.5151690321989832 2304 80-81 0.38377378275093577 2304 82-83 0.5008332280818308 2304 84-85 0.5091706866756383 2304 86-87 0.5892425366316658 2304 88-89 0.648816589292295 2304 90-91 0.5432653820870215 2304 92-93 0.5181284436363285 2304 94-95 0.6456574265014083 2304 96-97 0.7085347259026165 2304 98-99 0.7377847661908845 2304 100-101 0.5489204670766981 2202 1 0.11845375028574523 2202 2 0.10645766845182436 2202 3 0.08799827677821526 2202 4 0.12142594997214218 2202 5 0.16542425759160295 2202 6 0.13125455967064426 2202 7 0.15300817905440312 2202 8 0.12333775250266399 2202 9 0.15578116892830707 2202 10-11 0.15182450421110616 2202 12-13 0.12291646013011359 2202 14-15 0.17962416657527314 2202 16-17 0.23265700039360127 2202 18-19 0.2483531990222474 2202 20-21 0.19044064483294676 2202 22-23 0.27115860272621006 2202 24-25 0.2561071322437911 2202 26-27 0.22484822363160362 2202 28-29 0.296994388308768 2202 30-31 0.2590639138851074 2202 32-33 0.14932987950338372 2202 34-35 0.18157846543434886 2202 36-37 0.25997542582187094 2202 38-39 0.22195019169541297 2202 40-41 0.2726816247411179 2202 42-43 0.4486997132515924 2202 44-45 0.3078035607976801 2202 46-47 0.2727701170007393 2202 48-49 0.26410854820409213 2202 50-51 0.3630767072261989 2202 52-53 0.3832263490342882 2202 54-55 0.43885195417244915 2202 56-57 0.302692543213432 2202 58-59 0.25534500420126705 2202 60-61 0.22793292567478574 2202 62-63 0.40084987652463155 2202 64-65 0.38841870188878147 2202 66-67 0.344514235055847 2202 68-69 0.363308632583454 2202 70-71 0.38562598430417694 2202 72-73 0.2529233521812131 2202 74-75 0.09441441064085865 2202 76-77 0.011716212809471216 2202 78-79 0.25434350196277933 2202 80-81 0.36501137495897495 2202 82-83 0.537512533117642 2202 84-85 0.41291452957998587 2202 86-87 0.37327161949151133 2202 88-89 0.5065463583133081 2202 90-91 0.41768473851642085 2202 92-93 0.3900913540408766 2202 94-95 0.47892775062444315 2202 96-97 0.33089998207615423 2202 98-99 0.39701634604180924 2202 100-101 0.2818801283976242 1102 1 0.10336931688703999 1102 2 0.09866278552497931 1102 3 0.0797772865376487 1102 4 0.10129505931908511 1102 5 0.15734329318211593 1102 6 0.07907902556673463 1102 7 0.14630787603917383 1102 8 0.10366010335547315 1102 9 0.06295375763241395 1102 10-11 0.10115299157871505 1102 12-13 0.07286333908665199 1102 14-15 0.15712020657442594 1102 16-17 0.2097625257693423 1102 18-19 0.166972815669709 1102 20-21 0.16351054535932974 1102 22-23 0.2207648805733058 1102 24-25 0.14987685373476012 1102 26-27 0.21518284014424438 1102 28-29 0.23760819388424181 1102 30-31 0.28244680396801414 1102 32-33 0.23193808505057945 1102 34-35 0.22836133009150927 1102 36-37 0.2275962948295458 1102 38-39 0.2891750689927548 1102 40-41 0.31477479652215834 1102 42-43 0.1325867934735001 1102 44-45 0.33663313746910717 1102 46-47 0.26329927897126737 1102 48-49 0.27470570645019166 1102 50-51 0.26245751166120357 1102 52-53 0.25414815951468483 1102 54-55 0.3335913042951404 1102 56-57 0.23947280667304227 1102 58-59 0.2327744649326462 1102 60-61 0.1908162819800694 1102 62-63 0.1728656304355951 1102 64-65 0.23251735846152854 1102 66-67 0.22356541600455415 1102 68-69 0.39753858347224025 1102 70-71 0.32966933402355636 1102 72-73 0.2553895487563764 1102 74-75 0.21776443820217395 1102 76-77 0.15146366717838688 1102 78-79 0.3446586555548663 1102 80-81 0.2309440114795578 1102 82-83 0.45362190200145847 1102 84-85 0.275467535470618 1102 86-87 0.3319040361078933 1102 88-89 0.3459509671395935 1102 90-91 0.43783388612592944 1102 92-93 0.35271492558059947 1102 94-95 0.31878920209937434 1102 96-97 0.2974794508389209 1102 98-99 0.257642656794971 1102 100-101 0.2839623426549025 2305 1 0.010307353513645978 2305 2 -0.0026658994557706706 2305 3 0.040513368294334384 2305 4 0.06135966092578826 2305 5 0.04338133652198195 2305 6 -5.504025794138556E-4 2305 7 0.039695008034946966 2305 8 0.04408680422117328 2305 9 -0.019407580790648638 2305 10-11 0.06202750534977497 2305 12-13 0.0018074361649951243 2305 14-15 0.03672509660430734 2305 16-17 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74-75 0.11917306844917874 1213 76-77 -2.237990508433441 1213 78-79 -2.001798896786628 1213 80-81 -1.3429979976198894 1213 82-83 -0.917792538496542 1213 84-85 -0.6560457449467592 1213 86-87 -0.506538597785017 1213 88-89 -0.4664469704948502 1213 90-91 -1.7254808894189324 1213 92-93 -0.9886717329451571 1213 94-95 -0.5863068038181041 1213 96-97 -0.41803780374636546 1213 98-99 -2.297245777632348 1213 100-101 -0.6863552625150149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 13.0 10 15.0 11 14.0 12 30.0 13 106.0 14 267.0 15 643.0 16 1187.0 17 1916.0 18 2782.0 19 3763.0 20 4765.0 21 6144.0 22 7936.0 23 9969.0 24 12474.0 25 15601.0 26 19577.0 27 25333.0 28 34026.0 29 45476.0 30 52304.0 31 62169.0 32 83096.0 33 118866.0 34 180592.0 35 309396.0 36 581667.0 37 834854.0 38 193257.0 39 458.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.42138425154582 26.04622926635029 21.859161895356145 14.673224586747743 2 32.54612696074805 22.669749400710828 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28.053940981399567 31.21332708246205 18.584522653594526 22-23 21.438143782561678 28.66861425006986 30.913120428044127 18.980121539324333 24-25 21.73534127878873 27.3586721064069 31.40727403617813 19.498712578626236 26-27 21.55864427648529 27.66723540395361 30.967304442092487 19.806815877468612 28-29 21.502754433908343 28.642394323922655 30.43533510713945 19.419516135029554 30-31 20.568535398625293 28.97687088181368 30.682411911206447 19.77218180835457 32-33 20.36073098394106 29.56670422044978 30.93115621057667 19.141408585032487 34-35 21.13534963543186 28.89336195036384 31.052538368683596 18.918750045520703 36-37 20.48339730509759 29.108334678190655 31.813050393241394 18.59521762347036 38-39 21.117188852146445 29.16980280787419 30.425313455995983 19.28769488398338 40-41 21.03103176968583 28.50650564122517 30.890549785860564 19.57191280322843 42-43 20.917788330226696 29.040379888161077 30.823650050552725 19.2181817310595 44-45 20.515976143549636 28.46164151959233 31.40879519252313 19.6135871443349 46-47 21.187970518577696 28.357928020001854 30.729276481489006 19.72482497993144 48-49 20.78179716745157 29.886816900220616 30.082496200076285 19.248889732251527 50-51 21.507771327495238 29.12211198854207 29.604189995855855 19.765926688106838 52-53 20.863304664103865 28.823731007082714 29.499379744875036 20.813584583938383 54-55 21.80891209350519 28.927678981365485 29.70610929578761 19.55729962934172 56-57 21.256204683883396 29.116176985043584 29.52763414528952 20.099984185783498 58-59 20.996677396944094 29.280161460258352 29.983118599982518 19.740042542815033 60-61 21.1307014233108 29.500600066591005 29.948642120515146 19.420056389583053 62-63 20.629691789985685 29.287790976275673 29.725276073337408 20.357241160401234 64-65 21.134156283623607 28.998751907074872 29.705991785321118 20.161100023980406 66-67 21.194434820887118 29.144852293679826 29.45575444098678 20.204958444446277 68-69 21.314093592232116 29.659077575304867 29.219465263936108 19.807363568526913 70-71 20.54097728553441 30.755325437490306 28.98962263734911 19.714074639626176 72-73 20.409875888027358 30.5541229355413 28.919830498790784 20.116170677640557 74-75 20.909596384699007 29.747968118781888 29.018273989044424 20.324161507474678 76-77 20.86118154583583 29.278312762133012 29.243168364289996 20.61733732774116 78-79 20.68606179854761 29.334718426039846 29.485010825270138 20.494208950142415 80-81 20.15278476844421 29.743176225745476 29.41158543714591 20.6924535686644 82-83 19.565354890917057 29.46657783580576 29.630682373577983 21.3373848996992 84-85 19.74559065719219 29.304514535267074 29.937528559906035 21.012366247634702 86-87 20.019413168631342 29.277554439214214 30.549628866457684 20.15340352569676 88-89 20.23220194414201 29.849403093610267 29.876358733943803 20.04203622830392 90-91 20.477890879978407 29.612981123162623 29.37801756398616 20.53111043287281 92-93 19.924522122997917 30.032721268325908 29.58040236461302 20.462354244063157 94-95 19.588643040077365 30.41837415127454 29.609614088986426 20.38336871966167 96-97 19.882028466168745 30.933811891676328 29.182587455290935 20.001572186863996 98-99 20.39475147507388 30.156181811627714 28.788061814664918 20.66100489863349 100-101 20.43947288101019 30.055161296754434 28.224478026739387 21.280887795495993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 345.0 1 213.5 2 107.5 3 178.5 4 191.5 5 139.0 6 118.5 7 127.5 8 148.5 9 145.5 10 127.5 11 133.0 12 140.0 13 148.5 14 145.0 15 138.5 16 151.0 17 173.5 18 202.0 19 241.5 20 326.0 21 464.0 22 675.5 23 1023.5 24 1540.5 25 2409.0 26 4031.5 27 6643.0 28 9468.0 29 12264.5 30 15675.0 31 18841.0 32 24353.0 33 35643.5 34 52612.0 35 76956.0 36 112019.5 37 151269.0 38 184346.0 39 202158.0 40 202312.0 41 187481.5 42 169291.0 43 160103.0 44 147335.5 45 132393.0 46 123996.0 47 110571.0 48 92036.0 49 76674.5 50 63042.0 51 51735.0 52 42314.5 53 33610.0 54 27511.5 55 21740.5 56 15327.5 57 10074.5 58 6814.0 59 4919.0 60 3659.0 61 2724.0 62 1948.5 63 1374.0 64 1005.0 65 763.5 66 541.5 67 402.0 68 331.5 69 250.0 70 179.5 71 118.5 72 75.0 73 48.0 74 41.0 75 26.5 76 18.5 77 17.5 78 9.5 79 6.5 80 3.0 81 2.5 82 4.5 83 2.5 84 2.5 85 2.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16950952254817817 2 0.0075133121708373845 3 0.0023383267470463293 4 0.0 5 0.0012649964369267027 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.49165964849199E-4 12-13 0.0 14-15 1.9166612680707616E-5 16-17 0.0 18-19 5.7499838042122845E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.3416628876495332E-4 40-41 2.1083273948778377E-4 42-43 0.0015524956271373168 44-45 6.324982184633514E-4 46-47 0.0055583176774052084 48-49 0.0037566560854186922 50-51 0.007819977973728708 52-53 0.0037949893107801077 54-55 0.0193774454201954 56-57 0.011135801967491124 58-59 0.01943494525823752 60-61 0.009295807150143193 62-63 0.006018316381742191 64-65 0.012400798404417827 66-67 0.030992412704704216 68-69 0.03208490962750455 70-71 0.036780729734277914 72-73 0.03127991189491483 74-75 0.03442323637455088 76-77 0.034135737184340265 78-79 0.017403284314082515 80-81 0.0025299928738534054 82-83 0.0 84-85 0.0057883170295737 86-87 0.02604742663308165 88-89 0.027389089520731182 90-91 0.024494931005944333 92-93 0.0352282341071406 94-95 0.03150991124708332 96-97 0.03336907267711196 98-99 0.0397515546997876 100-101 0.02740825613341189 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2608703.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.51244611037795 #Duplication Level Percentage of deduplicated Percentage of total 1 87.2015463274837 40.55957224298712 2 5.574659265009467 5.185820772949441 3 1.917352721967165 2.675422954652528 4 1.0803730188792393 2.010031672789079 5 0.7936406641607425 1.845708431139055 6 0.5099302388410678 1.4230861652488396 7 0.3447530739862804 1.1224716142621807 8 0.30520035410463314 1.1356492018528015 9 0.21256181758465725 0.8898093076977323 >10 1.712618099562975 15.731136541819197 >50 0.18559787570730818 5.986258427820445 >100 0.14633966433926757 14.720707822015141 >500 0.012841050755047321 3.8852911656349685 >1k 0.0024209532292145783 1.7710966359652907 >5k 8.243719516226505E-5 0.2026456933265835 >10k+ 8.243719422413903E-5 0.8552913498397796 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 22306 0.8550609249117281 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5285 0.20259109603507952 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4995 0.19147446068026908 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2637 0.10108471527805196 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.6833257752990663E-4 0.0 0.0 2 3.449990282527371E-4 0.0 0.0 3 4.2166547897556754E-4 0.0 0.0 4 4.5999870433698276E-4 0.0 0.0 5 5.366651550598133E-4 0.0 0.0 6 6.899980565054742E-4 0.0 0.0 7 8.049977325897199E-4 0.0 0.0 8 0.0010733303101196265 0.0 0.0 9 0.0012649964369267027 0.0 0.0 10-11 0.0021083273948778376 0.0 0.0 12-13 0.003239157543039587 0.0 0.0 14-15 0.004542487205327705 0.0 0.0 16-17 0.006094982832465022 0.0 0.0 18-19 0.008088310551258615 0.0 0.0 20-21 0.010081638270052207 0.0 0.0 22-23 0.012419965017098536 0.0 0.0 24-25 0.01542912320796963 0.0 0.0 26-27 0.01857244768760568 0.0 0.0 28-29 0.021198273624862624 0.0 0.0 30-31 0.03127991189491483 0.0 0.0 32-33 0.035209067494459886 0.0 1.9166612680707616E-5 34-35 0.03946405550957698 0.0 3.833322536141523E-5 36-37 0.04362321046129053 0.0 3.833322536141523E-5 38-39 0.048184864279298946 0.0 3.833322536141523E-5 40-41 0.052286519392970375 0.0 3.833322536141523E-5 42-43 0.05795983674645983 0.0 3.833322536141523E-5 44-45 0.06409315280428626 0.0 3.833322536141523E-5 46-47 0.07059063450304615 0.0 3.833322536141523E-5 48-49 0.0775097816807816 0.0 3.833322536141523E-5 50-51 0.08469726143604694 0.0 3.833322536141523E-5 52-53 0.09198057425471584 0.0 3.833322536141523E-5 54-55 0.09918722062266191 0.0 3.833322536141523E-5 56-57 0.10854052761084723 0.0 3.833322536141523E-5 58-59 0.11950383006421199 0.0 3.833322536141523E-5 60-61 0.13021796655272755 0.0 3.833322536141523E-5 62-63 0.14200543335136273 0.0 3.833322536141523E-5 64-65 0.15664872543942335 0.0 3.833322536141523E-5 66-67 0.17092785188655052 0.0 3.833322536141523E-5 68-69 0.18769863798216968 0.0 3.833322536141523E-5 70-71 0.20594525325420332 0.0 3.833322536141523E-5 72-73 0.2254951981885251 0.0 3.833322536141523E-5 74-75 0.24884013243362696 0.0 3.833322536141523E-5 76-77 0.27490672567938934 0.0 3.833322536141523E-5 78-79 0.3062824706377077 0.0 3.833322536141523E-5 80-81 0.3396132100894582 0.0 3.833322536141523E-5 82-83 0.37723727078168734 0.0 3.833322536141523E-5 84-85 0.4201896497991531 0.0 3.833322536141523E-5 86-87 0.4697161769661016 0.0 3.833322536141523E-5 88-89 0.5249351880992201 0.0 3.833322536141523E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGG 30 1.2914001E-4 63.433464 1 CGCGTTG 30 1.3026026E-4 63.322823 6 CGGAAGA 4375 0.0 55.36331 4 TCGGAAG 4425 0.0 54.630405 3 GAAGAGC 4645 0.0 52.24645 6 GATCGGA 4790 0.0 50.95204 1 CGAGGCG 75 2.3537723E-9 50.746777 1 ATCGGAA 4950 0.0 48.842827 2 GTCGCGG 70 7.828385E-8 47.575104 1 TCGCGGG 80 4.2291504E-9 47.499405 2 CGACGGG 105 2.0008883E-11 45.30962 1 GCCGTCT 2760 0.0 43.28256 50-51 AGCGCCG 55 4.9654198E-5 43.18128 2 TATGCCG 2790 0.0 42.475437 48-49 CGTATGC 2805 0.0 42.33215 46-47 TGCCGTC 2795 0.0 42.3157 50-51 ATGCCGT 2825 0.0 42.28546 48-49 TCGTATG 2870 0.0 42.117725 44-45 CCGGGCG 105 1.0131771E-9 40.77866 1 CGTCTTC 2955 0.0 40.749004 52-53 >>END_MODULE