FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_35005-1.3410000003578e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_35005-1.3410000003578e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608703
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT223060.8550609249117281TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC52850.20259109603507952No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG49950.19147446068026908No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA26370.10108471527805196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG301.2914001E-463.4334641
CGCGTTG301.3026026E-463.3228236
CGGAAGA43750.055.363314
TCGGAAG44250.054.6304053
GAAGAGC46450.052.246456
GATCGGA47900.050.952041
CGAGGCG752.3537723E-950.7467771
ATCGGAA49500.048.8428272
GTCGCGG707.828385E-847.5751041
TCGCGGG804.2291504E-947.4994052
CGACGGG1052.0008883E-1145.309621
GCCGTCT27600.043.2825650-51
AGCGCCG554.9654198E-543.181282
TATGCCG27900.042.47543748-49
CGTATGC28050.042.3321546-47
TGCCGTC27950.042.315750-51
ATGCCGT28250.042.2854648-49
TCGTATG28700.042.11772544-45
CCGGGCG1051.0131771E-940.778661
CGTCTTC29550.040.74900452-53
Produced by FastQC (version 0.11.2)