##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34344-1.34100000035857.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4707634 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69145583535169 33.0 33.0 33.0 33.0 33.0 2 32.71957845490962 33.0 33.0 33.0 33.0 33.0 3 32.74845835508878 33.0 33.0 33.0 33.0 33.0 4 36.687841493200196 37.0 37.0 37.0 37.0 37.0 5 36.65582434828196 37.0 37.0 37.0 37.0 37.0 6 36.52451295066694 37.0 37.0 37.0 37.0 37.0 7 36.468808322822035 37.0 37.0 37.0 37.0 37.0 8 36.402029342128124 37.0 37.0 37.0 37.0 37.0 9 36.428313033681036 37.0 37.0 37.0 37.0 37.0 10-11 36.36964014194817 37.0 37.0 37.0 37.0 37.0 12-13 36.34283410732441 37.0 37.0 37.0 37.0 37.0 14-15 38.16389581263114 40.0 37.0 40.0 37.0 40.0 16-17 38.15407835443452 40.0 37.0 40.0 37.0 40.0 18-19 38.165921458635054 40.0 37.0 40.0 37.0 40.0 20-21 38.10355509795366 40.0 37.0 40.0 37.0 40.0 22-23 37.98192117314133 40.0 37.0 40.0 33.0 40.0 24-25 37.97525444841294 40.0 37.0 40.0 33.0 40.0 26-27 37.8908171280945 40.0 37.0 40.0 33.0 40.0 28-29 37.76036837188278 40.0 37.0 40.0 33.0 40.0 30-31 37.68404595599403 40.0 37.0 40.0 33.0 40.0 32-33 37.551794489546126 40.0 37.0 40.0 33.0 40.0 34-35 37.45480627848299 40.0 37.0 40.0 33.0 40.0 36-37 37.39685721107461 40.0 37.0 40.0 33.0 40.0 38-39 37.27562263761371 40.0 37.0 40.0 33.0 40.0 40-41 37.13260482866765 40.0 37.0 40.0 33.0 40.0 42-43 37.01317137228595 38.5 37.0 40.0 33.0 40.0 44-45 36.898434436491875 37.0 37.0 40.0 33.0 40.0 46-47 36.83677299467206 37.0 37.0 40.0 33.0 40.0 48-49 36.613773500658716 37.0 37.0 40.0 33.0 40.0 50-51 36.453286194296325 37.0 37.0 40.0 33.0 40.0 52-53 36.32467849029895 37.0 37.0 40.0 33.0 40.0 54-55 36.13369136173288 37.0 37.0 40.0 33.0 40.0 56-57 35.9489292285679 37.0 37.0 40.0 30.0 40.0 58-59 35.73208101989237 37.0 37.0 40.0 27.0 40.0 60-61 35.47343803702667 37.0 33.0 40.0 27.0 40.0 62-63 35.242025187174704 37.0 33.0 40.0 27.0 40.0 64-65 34.92273443517487 37.0 33.0 37.0 27.0 40.0 66-67 34.50876236342927 37.0 33.0 37.0 27.0 40.0 68-69 34.22537361655558 37.0 33.0 37.0 27.0 40.0 70-71 33.8950079806544 37.0 33.0 37.0 27.0 40.0 72-73 33.414812302740614 37.0 33.0 37.0 27.0 37.0 74-75 32.91286365932441 37.0 33.0 37.0 24.5 37.0 76-77 30.235689839099642 33.0 30.0 35.0 22.0 37.0 78-79 32.613656456725394 35.0 33.0 37.0 27.0 37.0 80-81 32.99786761247795 37.0 33.0 37.0 27.0 37.0 82-83 32.940419646047246 37.0 33.0 37.0 27.0 37.0 84-85 32.81390949253914 37.0 33.0 37.0 27.0 37.0 86-87 32.655884144774205 37.0 33.0 37.0 27.0 37.0 88-89 32.512875045086346 33.0 33.0 37.0 22.0 37.0 90-91 32.36064050433828 33.0 33.0 37.0 22.0 37.0 92-93 32.19347925093582 33.0 33.0 37.0 22.0 37.0 94-95 32.09539993975743 33.0 33.0 37.0 22.0 37.0 96-97 31.956278461749577 33.0 33.0 37.0 22.0 37.0 98-99 31.653171104635575 33.0 33.0 37.0 22.0 37.0 100-101 30.058887012031946 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1527592857468818 2306 2 0.1720405028718872 2306 3 0.14243928879161416 2306 4 0.1635530747536933 2306 5 0.16547763345208466 2306 6 0.20761404950788886 2306 7 0.2272287534248676 2306 8 0.21238904325439023 2306 9 0.2053026144285468 2306 10-11 0.2486994475520845 2306 12-13 0.3139288905219857 2306 14-15 0.4741022843879179 2306 16-17 0.4770141705219544 2306 18-19 0.5530285479052921 2306 20-21 0.49489515354395763 2306 22-23 0.5768274507999891 2306 24-25 0.5297473749693609 2306 26-27 0.664592576961752 2306 28-29 0.5717275387960896 2306 30-31 0.5901076226674817 2306 32-33 0.5854260800089719 2306 34-35 0.6090161076171938 2306 36-37 0.606424504285215 2306 38-39 0.6245420956257988 2306 40-41 0.6041691649098269 2306 42-43 0.6979329304913762 2306 44-45 0.7247199009564937 2306 46-47 0.6367094248520431 2306 48-49 0.750474285364831 2306 50-51 0.7359872519474493 2306 52-53 0.7040465830949003 2306 54-55 0.7344765093856225 2306 56-57 0.6816121828808619 2306 58-59 0.6984249495521837 2306 60-61 0.6542410372627216 2306 62-63 0.7122474155177727 2306 64-65 0.688933957421753 2306 66-67 0.6247187998729373 2306 68-69 0.716318484080027 2306 70-71 0.7006956991488025 2306 72-73 0.6215958338256016 2306 74-75 0.632354816448121 2306 76-77 0.21876630728633017 2306 78-79 0.67435723405098 2306 80-81 0.7046969618295549 2306 82-83 0.7476628291350522 2306 84-85 0.7119603901275795 2306 86-87 0.6776975693195268 2306 88-89 0.8111694909708262 2306 90-91 0.9081594937304089 2306 92-93 0.8493020853652595 2306 94-95 0.7291319473313251 2306 96-97 0.840719568250357 2306 98-99 0.7421431378848204 2306 100-101 0.5268611475229399 2307 1 0.12380457827309499 2307 2 0.08377357186139989 2307 3 0.09663685735259264 2307 4 0.12897047561996544 2307 5 0.1732464080306002 2307 6 0.21072596933041865 2307 7 0.20273544702443758 2307 8 0.24108406456615228 2307 9 0.16250317123785862 2307 10-11 0.19281131848123323 2307 12-13 0.20999223045752302 2307 14-15 0.47364936152250436 2307 16-17 0.40637725755409093 2307 18-19 0.34231653566468623 2307 20-21 0.37319383888725355 2307 22-23 0.40387610738247304 2307 24-25 0.4127601970185495 2307 26-27 0.48181492248264135 2307 28-29 0.42296487684874506 2307 30-31 0.5398259944028894 2307 32-33 0.5314385013241818 2307 34-35 0.41493455537701607 2307 36-37 0.4286994053959532 2307 38-39 0.4896057744595623 2307 40-41 0.4738354723859146 2307 42-43 0.3422393443441152 2307 44-45 0.4420259284556707 2307 46-47 0.4198689629917567 2307 48-49 0.42719449347806915 2307 50-51 0.3993481828774037 2307 52-53 0.4064204765226691 2307 54-55 0.5285730052641995 2307 56-57 0.41646338402822636 2307 58-59 0.43255503326532363 2307 60-61 0.4273403430726006 2307 62-63 0.36065416242070825 2307 64-65 0.4231188664321337 2307 66-67 0.4989025320867668 2307 68-69 0.4801665153065926 2307 70-71 0.15546938271851474 2307 72-73 0.04322423982472401 2307 74-75 0.16981265789168276 2307 76-77 -0.20896508303047767 2307 78-79 0.14402326590262504 2307 80-81 0.1342794099690181 2307 82-83 0.13500978566596444 2307 84-85 0.22403675535537104 2307 86-87 0.2908231289569798 2307 88-89 0.2830217040194185 2307 90-91 0.2552301691109662 2307 92-93 0.17960730468562502 2307 94-95 0.3511573004661557 2307 96-97 0.31486749325366503 2307 98-99 0.2305256274398566 2307 100-101 0.046611915915956814 2201 1 0.09341495483073459 2201 2 0.07913201346730858 2201 3 0.09830237359722105 2201 4 0.10700063329008458 2201 5 0.15097016719452228 2201 6 0.17288746698886825 2201 7 0.18708669620834684 2201 8 0.16947247279735933 2201 9 0.1961955857619131 2201 10-11 0.2162972675503454 2201 12-13 0.23135612846949982 2201 14-15 0.4616351864124866 2201 16-17 0.41875000509417504 2201 18-19 0.46176294593259826 2201 20-21 0.43720345633246893 2201 22-23 0.43855396013842096 2201 24-25 0.39896826683462905 2201 26-27 0.4591094018689077 2201 28-29 0.4932708226617919 2201 30-31 0.5362696017111048 2201 32-33 0.5672783530235748 2201 34-35 0.5914502052699433 2201 36-37 0.5713143084697876 2201 38-39 0.6129603490692119 2201 40-41 0.6475107842458101 2201 42-43 0.594857073635275 2201 44-45 0.59306585599154 2201 46-47 0.6801648249610395 2201 48-49 0.743391418321039 2201 50-51 0.7530788728149815 2201 52-53 0.7496818067261941 2201 54-55 0.6437418286607084 2201 56-57 0.7876966862960089 2201 58-59 0.7557590977853081 2201 60-61 0.7150210776980543 2201 62-63 0.737018263936946 2201 64-65 0.6219707701521955 2201 66-67 0.720685057051341 2201 68-69 0.6664847889093508 2201 70-71 0.6393988277914673 2201 72-73 0.6730028001940553 2201 74-75 0.5151799457031885 2201 76-77 0.43395170200091115 2201 78-79 0.4623226700919858 2201 80-81 0.566099616446941 2201 82-83 0.563047927763435 2201 84-85 0.5202950907237422 2201 86-87 0.5792577638038878 2201 88-89 0.6132500918669734 2201 90-91 0.7094658507173364 2201 92-93 0.7853437575315994 2201 94-95 0.7970139018121074 2201 96-97 0.653048848359461 2201 98-99 0.674029055033035 2201 100-101 0.5968681236265247 1101 1 0.0977936098512231 1101 2 0.06141204330837269 1101 3 0.09488688662774081 1101 4 0.106282724341618 1101 5 0.13015534280557262 1101 6 0.10490607003692531 1101 7 0.10381052801901092 1101 8 0.17639623522074288 1101 9 0.13598964611978204 1101 10-11 0.10782254062104357 1101 12-13 0.14403522589060458 1101 14-15 0.37390830020083143 1101 16-17 0.3523227472297137 1101 18-19 0.3129826428507627 1101 20-21 0.333757629433336 1101 22-23 0.3806141728807333 1101 24-25 0.2800227267816169 1101 26-27 0.38319558722365343 1101 28-29 0.38663422381779355 1101 30-31 0.2664982396115221 1101 32-33 0.39851210880301124 1101 34-35 0.455072538904858 1101 36-37 0.46114353865642954 1101 38-39 0.5670088249299141 1101 40-41 0.48504141328914585 1101 42-43 0.49690658350255745 1101 44-45 0.6031661954450129 1101 46-47 0.5438162286929895 1101 48-49 0.5462328048875662 1101 50-51 0.5950681563345981 1101 52-53 0.5479741085926193 1101 54-55 0.6612993509451357 1101 56-57 0.6976312672399061 1101 58-59 0.6563494339054685 1101 60-61 0.4853372886988865 1101 62-63 0.5659822392387 1101 64-65 0.45461267961766083 1101 66-67 0.4970394129174025 1101 68-69 0.5803465086708925 1101 70-71 0.602657943942603 1101 72-73 0.4494930735028575 1101 74-75 0.5502843780993416 1101 76-77 0.36175908653117617 1101 78-79 0.6198897614723151 1101 80-81 0.6342429624105677 1101 82-83 0.6648224735148247 1101 84-85 0.6791888397458621 1101 86-87 0.5293008700866082 1101 88-89 0.6765259879493044 1101 90-91 0.5483514365296784 1101 92-93 0.6356963401670939 1101 94-95 0.7784095839276546 1101 96-97 0.6482522369985375 1101 98-99 0.6897018735852889 1101 100-101 0.594114355511568 2304 1 0.07261092432301552 2304 2 0.07626872737754553 2304 3 0.08332404402869997 2304 4 0.12581428319020205 2304 5 0.16965372685884716 2304 6 0.18829951740352158 2304 7 0.15431878707585156 2304 8 0.16380652266771278 2304 9 0.1554991971188997 2304 10-11 0.22111515943286975 2304 12-13 0.17138882274755218 2304 14-15 0.3802640041055909 2304 16-17 0.3489744159545225 2304 18-19 0.36878615351030675 2304 20-21 0.35628563849730455 2304 22-23 0.35153907313219435 2304 24-25 0.3145934310735541 2304 26-27 0.36293238964614716 2304 28-29 0.3709478845790173 2304 30-31 0.4233824987009669 2304 32-33 0.43956135340395264 2304 34-35 0.47767211423487765 2304 36-37 0.37344718992167714 2304 38-39 0.5288797774214942 2304 40-41 0.4510279558967554 2304 42-43 0.4950458605854564 2304 44-45 0.5356340089651681 2304 46-47 0.4724077263271482 2304 48-49 0.544115812790011 2304 50-51 0.41342107257842997 2304 52-53 0.4997934241484998 2304 54-55 0.5544670140783197 2304 56-57 0.5584231830232014 2304 58-59 0.5178658154889817 2304 60-61 0.5238005294769863 2304 62-63 0.4882327863782905 2304 64-65 0.45275680657849904 2304 66-67 0.4872646673322123 2304 68-69 0.5453646651466997 2304 70-71 0.5000626886719246 2304 72-73 0.5644644836761827 2304 74-75 0.519660903875014 2304 76-77 0.32929644893117427 2304 78-79 0.5438145761376987 2304 80-81 0.5864364929179331 2304 82-83 0.6288566753431084 2304 84-85 0.5546715656217955 2304 86-87 0.5947699837000968 2304 88-89 0.6682525037399429 2304 90-91 0.5496267098956977 2304 92-93 0.632999115193897 2304 94-95 0.7004777104483395 2304 96-97 0.5378022987427826 2304 98-99 0.6561709179148103 2304 100-101 0.5498108428142636 2202 1 0.1117882188492132 2202 2 0.07864225684530624 2202 3 0.07874413505568612 2202 4 0.09141637063863328 2202 5 0.13504814985053315 2202 6 0.12927941911491558 2202 7 0.14713975153335213 2202 8 0.1177960048393416 2202 9 0.1317891409786469 2202 10-11 0.13735874851228402 2202 12-13 0.13783761884717904 2202 14-15 0.11578029381035293 2202 16-17 0.20499796926606706 2202 18-19 0.16873266552229893 2202 20-21 0.16849841176535563 2202 22-23 0.16719270033066636 2202 24-25 0.13363190036069028 2202 26-27 0.2064618706931185 2202 28-29 0.10293885980939166 2202 30-31 0.1297828146697455 2202 32-33 0.14661289005451295 2202 34-35 0.23290733961722765 2202 36-37 0.24769628808675037 2202 38-39 0.1792962575665129 2202 40-41 0.15352249750957014 2202 42-43 0.22533865861383617 2202 44-45 0.0861645495879273 2202 46-47 0.14830206718288963 2202 48-49 0.13602903118616183 2202 50-51 0.20069031153911254 2202 52-53 0.20911355108506058 2202 54-55 0.14833810094272337 2202 56-57 0.21093866819423823 2202 58-59 0.18253210667705133 2202 60-61 0.0996234054216103 2202 62-63 0.22011550156670978 2202 64-65 0.19452373001407608 2202 66-67 0.1508128248968461 2202 68-69 0.19245519312362802 2202 70-71 -0.020967765387069903 2202 72-73 -0.16450960077373367 2202 74-75 -0.12055987133772561 2202 76-77 -0.22869528965425445 2202 78-79 0.057511353265027765 2202 80-81 0.14134746951206267 2202 82-83 0.2286237280708363 2202 84-85 0.1757440396316312 2202 86-87 0.15729757451344284 2202 88-89 0.19029770197536777 2202 90-91 0.2655371945597409 2202 92-93 0.2334689726315773 2202 94-95 0.24297044108539723 2202 96-97 0.16478169745455773 2202 98-99 0.057858069860987626 2202 100-101 0.10172319335895352 1102 1 0.07038560138663996 1102 2 0.038157970086899695 1102 3 0.07074181631596588 1102 4 0.08017335682587401 1102 5 0.10737175436715773 1102 6 0.061142620720737284 1102 7 0.11549300619626734 1102 8 0.1321369931723737 1102 9 0.040923981285679645 1102 10-11 0.056188748074738726 1102 12-13 0.09406736126668136 1102 14-15 0.206344154558586 1102 16-17 0.14932711535313814 1102 18-19 0.16458826779267355 1102 20-21 0.15869153565093796 1102 22-23 0.12872022319637466 1102 24-25 0.11166720728949286 1102 26-27 0.1532988207788577 1102 28-29 0.12833525400860424 1102 30-31 0.05429176535199787 1102 32-33 0.147259436812611 1102 34-35 0.13105315044214194 1102 36-37 0.1305227789890111 1102 38-39 0.0864881889425888 1102 40-41 0.1843026928419036 1102 42-43 0.06802721091091968 1102 44-45 0.11513102883434811 1102 46-47 0.02033406221815426 1102 48-49 0.10873671904071358 1102 50-51 0.009489755310838177 1102 52-53 0.04108090956776067 1102 54-55 0.0280088171189945 1102 56-57 0.1416102988176462 1102 58-59 -0.007165547113565651 1102 60-61 -0.13085025464276612 1102 62-63 -0.015144572174186521 1102 64-65 -0.08831678698513912 1102 66-67 -0.13839225204539218 1102 68-69 0.027137510765591344 1102 70-71 -0.03633249051052445 1102 72-73 -0.00127427924277157 1102 74-75 -0.08149346958111892 1102 76-77 -0.17575376404468557 1102 78-79 0.04110418557348794 1102 80-81 0.13526686197947413 1102 82-83 0.1518455723802603 1102 84-85 0.07530860925764671 1102 86-87 0.14442440719421512 1102 88-89 0.1658389915167291 1102 90-91 0.18503105505166673 1102 92-93 0.179012181942376 1102 94-95 0.1995798978463057 1102 96-97 0.10078288892844256 1102 98-99 -0.01702235555888265 1102 100-101 0.027240637888230168 2305 1 0.02165617091531402 2305 2 -0.004758130329321375 2305 3 0.04755759023992567 2305 4 0.05396805658713788 2305 5 0.04007405775954709 2305 6 0.01488229641795158 2305 7 -0.03679060014118818 2305 8 -0.03362513094314323 2305 9 -0.06558957754282346 2305 10-11 -0.02424221992076525 2305 12-13 0.010369507716660564 2305 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119541.0 31 120355.0 32 155011.0 33 217750.0 34 331438.0 35 560737.0 36 1024871.0 37 1418054.0 38 338781.0 39 215.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.093227694742694 24.9403383694878 23.91402000970758 15.052413926061925 2 28.82046597589926 23.909800151890938 33.61454754026968 13.655186331940113 3 36.9902990166464 19.07572578938916 27.490821155060242 16.443154038904197 4 27.915572875886273 19.2006430406442 34.44199357894008 18.44179050452945 5 33.613364514311094 18.11316281793732 32.023776168219236 16.249696499532348 6 26.980687113739087 19.39373366748562 37.364013430100975 16.26156578867431 7 31.91713714362671 21.611790551262054 29.097950265462437 17.37312203964879 8 27.795491323242207 28.67827023086332 26.42297595777412 17.10326248812036 9 31.86439302630578 23.82438821709589 25.714743329664113 18.59647542693421 10-11 24.990610974878198 28.836988680489352 28.875840551411926 17.296559793220528 12-13 23.97824469786734 27.095596216698244 28.8148887530339 20.111270332400522 14-15 22.96200659652101 27.557086544341924 29.554353536055167 19.926553323081897 16-17 23.18990813644391 27.106918252353516 30.3875789834129 19.31559462778967 18-19 21.176700002963273 27.357164637886378 31.684765470704324 19.781369888446026 20-21 22.494218964345997 27.510008212193217 31.215234659278952 18.780538164181838 22-23 21.34787878581895 29.947039213328818 29.80753176648822 18.89755023436401 24-25 21.390182414350818 27.090976061435533 31.14436041544436 20.374481108769288 26-27 21.2718002291597 27.32993898846002 29.604499840047037 21.79376094233324 28-29 22.026924777924535 28.864839535104046 29.364984618600342 19.743251068371077 30-31 20.43065582413586 28.16174749353922 30.543177315823616 20.864419366501306 32-33 20.361406600428154 29.250649052156565 29.77395863824588 20.613985709169405 34-35 20.589546680986672 29.43271503264697 31.102078029005654 18.875660257360703 36-37 20.489318040582386 28.437398535803602 31.049942609168706 20.023340814445305 38-39 22.248390165234028 29.378268367113275 29.234597225862885 19.13874424178981 40-41 20.832333626793005 30.497353074470674 29.014250584396844 19.656062714339473 42-43 20.91253690732275 29.381123184930818 30.563899388824613 19.142440518921816 44-45 20.510670965662943 28.231955468703283 30.805925851342202 20.45144771429157 46-47 21.90932850572305 28.079529384473318 29.473344653178728 20.537797456624904 48-49 20.827655693549037 29.639812904221486 30.13155696363737 19.40097443859211 50-51 22.051785583788092 28.390382847246887 29.784900162642526 19.772931406322495 52-53 20.604838876708985 28.126821918218532 28.854521745438678 22.413817459633805 54-55 22.0779387715601 28.337578019614295 29.857304603319246 19.727178605506356 56-57 20.864503098573415 28.35401014066985 29.59076269169107 21.190724069065666 58-59 20.79556068114428 28.28860034317271 29.982176350658257 20.933662625024752 60-61 21.783176482472463 28.61655873045817 29.931040431120255 19.669224355949112 62-63 20.56121809510659 28.583024419259246 29.829575026288673 21.026182459345492 64-65 21.96016492008616 28.44338238715175 29.715103461711443 19.881349231050642 66-67 21.05727387084162 30.517268856137488 28.36308157818689 20.062375694834007 68-69 21.235376470586985 30.492492517378878 28.27956396327595 19.992567048758183 70-71 20.948159472079997 31.171690132599437 28.196370719981417 19.683779675339146 72-73 20.631061772172988 31.585769374934657 28.01085902392878 19.772309828963575 74-75 21.089580533933898 30.924749274568853 27.958118098098073 20.027552093399173 76-77 21.178183545957356 30.260984996064067 28.31273745624866 20.248094001729918 78-79 20.941012377324896 30.0085249137284 28.82730649375344 20.223156215193256 80-81 20.623148174111357 30.162234180404273 28.689621023061846 20.524996622422524 82-83 19.865074472654413 30.146183836721374 28.979653048643968 21.009088641980238 84-85 19.98655084231962 29.903196230242102 29.037328369756455 21.072924557681823 86-87 20.003019314048395 30.0005821900417 29.46281069878316 20.533587797126742 88-89 20.155559255080867 30.207681836479306 29.21548189722754 20.42127701121229 90-91 20.36379483201285 29.894273063168125 28.920952916088066 20.82097918873096 92-93 19.965490386076308 30.568144268885995 28.76691879509598 20.699446549941722 94-95 19.67470453110911 30.942411920304224 28.740683809374662 20.642199739212003 96-97 20.079610584924776 31.076750957414767 28.44608489210391 20.39755356555655 98-99 20.375664334445766 30.380395786553176 28.16321161576274 21.080728263238317 100-101 20.3966917283328 30.468989123040707 27.723152111589418 21.411167037037078 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 57.0 1 43.0 2 65.0 3 187.0 4 224.0 5 148.0 6 114.5 7 95.5 8 89.5 9 104.5 10 104.5 11 96.5 12 111.5 13 122.0 14 128.0 15 140.5 16 171.5 17 219.0 18 278.5 19 365.0 20 537.5 21 831.0 22 1286.5 23 1973.0 24 2953.5 25 4550.5 26 7162.0 27 10681.0 28 14655.5 29 19124.0 30 24737.5 31 31201.5 32 43694.5 33 66789.5 34 99954.5 35 139925.5 36 194174.5 37 260939.0 38 319744.0 39 342629.0 40 338647.5 41 317147.0 42 290792.0 43 287874.0 44 279738.5 45 258115.5 46 240954.0 47 212239.0 48 170571.5 49 140710.5 50 118485.5 51 99249.5 52 84323.0 53 68651.5 54 52895.0 55 39395.5 56 29247.5 57 21684.5 58 16899.5 59 13309.0 60 10047.5 61 7439.5 62 5482.5 63 3954.5 64 2809.5 65 1977.0 66 1335.5 67 939.5 68 705.0 69 510.0 70 343.5 71 236.0 72 160.0 73 101.0 74 76.5 75 51.5 76 36.0 77 28.0 78 22.5 79 18.0 80 12.5 81 11.5 82 8.0 83 2.5 84 4.0 85 4.5 86 3.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17450379532478522 2 0.006563806787018702 3 0.0014869465213310975 4 0.0 5 0.0013382518691979878 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.124209316187282E-4 12-13 0.0 14-15 2.1242093161872823E-5 16-17 0.0 18-19 5.310523290468205E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.0621046580936411E-5 38-39 1.6993674529498258E-4 40-41 5.310523290468205E-5 42-43 0.0013913571021026698 44-45 4.991891893040113E-4 46-47 0.005671638874220043 48-49 0.004365250144764865 50-51 0.008029511215187928 52-53 0.004216555492631755 54-55 0.020498619901207275 56-57 0.011651288099287243 58-59 0.020519861994369146 60-61 0.00967577343523307 62-63 0.006032754457971881 64-65 0.013318792412494259 66-67 0.031310845320600536 68-69 0.033190770565426284 70-71 0.03758788384993396 72-73 0.03190562392913298 74-75 0.03482641173889049 76-77 0.034741443366243 78-79 0.01787522139571598 80-81 0.002506566993100993 82-83 0.0 84-85 0.005862817712676899 86-87 0.026913732036092868 88-89 0.028135152392900552 90-91 0.025862248424580164 92-93 0.036090316282021924 94-95 0.03212866590733264 96-97 0.034019212198739324 98-99 0.040158177122520566 100-101 0.02781652099547246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4707634.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.65503224727354 #Duplication Level Percentage of deduplicated Percentage of total 1 90.64872316718764 42.29219102539311 2 4.399872260869722 4.105523644295223 3 1.3912169768048295 1.9472181874735115 4 0.6866813047121663 1.2814855365978393 5 0.46209238499100924 1.0779467561487537 6 0.3584767007956889 1.0034845221311457 7 0.2083540862720306 0.680453662971096 8 0.21145722106539414 0.7892434774179848 9 0.2113445154883833 0.8874256666855432 >10 1.1102509618447969 10.107886073439147 >50 0.13016756881088434 4.28691598325246 >100 0.14408743994496498 14.232528424961208 >500 0.028906077920491515 9.2827085135811 >1k 0.008186699600637002 5.353537063146567 >5k 1.3697537479301543E-4 0.44418845706874954 >10k+ 4.5658316665073045E-5 2.227263005436372 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 104557 2.2210095347259364 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7752 0.16466870619083812 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7226 0.15349536518769302 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5874 0.12477605523284095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012532834965504965 0.0 0.0 2 0.0013594939623598607 0.0 0.0 3 0.0015081886144929703 0.0 0.0 4 0.0015931569871404617 0.0 0.0 5 0.0018480621050829356 0.0 0.0 6 0.0020179988503779183 0.0 0.0 7 0.0024640828067772473 0.0 0.0 8 0.0027827142042053396 0.0 0.0 9 0.0029314088563384493 0.0 0.0 10-11 0.004949407706716367 0.0 0.0 12-13 0.007031132836579904 0.0 2.1242093161872823E-5 14-15 0.009250931571995614 0.0 5.310523290468206E-5 16-17 0.011343277748440087 0.0 6.372627948561847E-5 18-19 0.014593318002206628 0.0 6.372627948561847E-5 20-21 0.018533726283734037 0.0 6.372627948561847E-5 22-23 0.022590966077651747 0.0 7.434732606655489E-5 24-25 0.027179258200616275 0.0 8.496837264749129E-5 26-27 0.03242605521159886 0.0 1.062104658093641E-4 28-29 0.03808707303923797 0.0 1.062104658093641E-4 30-31 0.057247441071247254 0.0 1.062104658093641E-4 32-33 0.06375814262536128 0.0 1.062104658093641E-4 34-35 0.07020511789998968 0.0 1.062104658093641E-4 36-37 0.0776186084134833 0.0 1.062104658093641E-4 38-39 0.08490464636800567 0.0 1.062104658093641E-4 40-41 0.09353955723830697 0.0 1.2745255897123693E-4 42-43 0.10191956299066579 0.0 1.2745255897123693E-4 44-45 0.11195645200965071 0.0 1.2745255897123693E-4 46-47 0.1219614778888928 0.0 1.2745255897123693E-4 48-49 0.1323701035382105 0.0 1.2745255897123693E-4 50-51 0.14454182291996362 0.0 1.2745255897123693E-4 52-53 0.15805179417091472 0.0 1.2745255897123693E-4 54-55 0.17217778612356016 0.0 1.2745255897123693E-4 56-57 0.18673924098602396 0.0 1.2745255897123693E-4 58-59 0.20355235772364633 0.0 1.2745255897123693E-4 60-61 0.22151254749200977 0.0 1.2745255897123693E-4 62-63 0.24109775738725653 0.0 1.2745255897123693E-4 64-65 0.2611609143786454 0.0 1.2745255897123693E-4 66-67 0.28416610127295366 0.0 1.2745255897123693E-4 68-69 0.3094229500424205 0.0 1.2745255897123693E-4 70-71 0.3376961760408732 0.0 1.2745255897123693E-4 72-73 0.369463726364454 0.0 1.2745255897123693E-4 74-75 0.402527044370909 0.0 1.2745255897123693E-4 76-77 0.4399343704289671 0.0 1.2745255897123693E-4 78-79 0.48145204151384746 0.0 1.2745255897123693E-4 80-81 0.5267614262281222 0.0 1.2745255897123693E-4 82-83 0.5755120300346204 0.0 1.2745255897123693E-4 84-85 0.6297537149234627 0.0 1.2745255897123693E-4 86-87 0.689964427990791 0.0 1.2745255897123693E-4 88-89 0.7538287810819617 0.0 1.2745255897123693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 16575 0.0 68.79689 3 CGGAAGA 16675 0.0 68.24118 4 GATCGGA 17075 0.0 67.12249 1 ATCGGAA 17125 0.0 66.674805 2 GAAGAGC 17570 0.0 64.79138 6 GGAAGAG 20260 0.0 56.423786 5 TTGTGTT 56955 0.0 45.50064 1 GCCGTCT 12870 0.0 44.124485 50-51 ATGCCGT 12960 0.0 43.77932 48-49 TCGTATG 13145 0.0 43.502296 44-45 TGCCGTC 12895 0.0 43.449547 50-51 TATGCCG 12960 0.0 43.449463 48-49 CGTATGC 13035 0.0 43.269127 46-47 TCTCGTA 13005 0.0 43.148666 42-43 CGTCTTC 13170 0.0 43.120518 52-53 CTCGTAT 12945 0.0 42.96365 44-45 AATCTCG 12940 0.0 42.814175 40-41 GTATGCC 13475 0.0 42.420216 46-47 ATCTCGT 13105 0.0 42.221428 42-43 CCGTCTT 13525 0.0 41.532112 52-53 >>END_MODULE