FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34344-1.34100000035857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34344-1.34100000035857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4707634
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1045572.2210095347259364TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC77520.16466870619083812No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA72260.15349536518769302No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG58740.12477605523284095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG165750.068.796893
CGGAAGA166750.068.241184
GATCGGA170750.067.122491
ATCGGAA171250.066.6748052
GAAGAGC175700.064.791386
GGAAGAG202600.056.4237865
TTGTGTT569550.045.500641
GCCGTCT128700.044.12448550-51
ATGCCGT129600.043.7793248-49
TCGTATG131450.043.50229644-45
TGCCGTC128950.043.44954750-51
TATGCCG129600.043.44946348-49
CGTATGC130350.043.26912746-47
TCTCGTA130050.043.14866642-43
CGTCTTC131700.043.12051852-53
CTCGTAT129450.042.9636544-45
AATCTCG129400.042.81417540-41
GTATGCC134750.042.42021646-47
ATCTCGT131050.042.22142842-43
CCGTCTT135250.041.53211252-53
Produced by FastQC (version 0.11.2)