##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34311-1.34100000035764.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2570498 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68993167860858 33.0 33.0 33.0 33.0 33.0 2 32.71758312980597 33.0 33.0 33.0 33.0 33.0 3 32.74101283097672 33.0 33.0 33.0 33.0 33.0 4 36.69097700134371 37.0 37.0 37.0 37.0 37.0 5 36.65246189648854 37.0 37.0 37.0 37.0 37.0 6 36.53816303299983 37.0 37.0 37.0 37.0 37.0 7 36.49697996263759 37.0 37.0 37.0 37.0 37.0 8 36.42072897936509 37.0 37.0 37.0 37.0 37.0 9 36.43849635362486 37.0 37.0 37.0 37.0 37.0 10-11 36.390253950790864 37.0 37.0 37.0 37.0 37.0 12-13 36.37284779058377 37.0 37.0 37.0 37.0 37.0 14-15 38.205047037577934 40.0 37.0 40.0 37.0 40.0 16-17 38.1976506108933 40.0 37.0 40.0 37.0 40.0 18-19 38.215261595223964 40.0 37.0 40.0 37.0 40.0 20-21 38.14134089970115 40.0 37.0 40.0 37.0 40.0 22-23 38.0563842103748 40.0 37.0 40.0 37.0 40.0 24-25 38.033537275656315 40.0 37.0 40.0 35.0 40.0 26-27 37.93955957172501 40.0 37.0 40.0 33.0 40.0 28-29 37.8350949893756 40.0 37.0 40.0 33.0 40.0 30-31 37.77738827262266 40.0 37.0 40.0 33.0 40.0 32-33 37.66207501425794 40.0 37.0 40.0 33.0 40.0 34-35 37.595106862561266 40.0 37.0 40.0 33.0 40.0 36-37 37.51904105741378 40.0 37.0 40.0 33.0 40.0 38-39 37.42488187113936 40.0 37.0 40.0 33.0 40.0 40-41 37.33825838417303 40.0 37.0 40.0 33.0 40.0 42-43 37.22952556275088 40.0 37.0 40.0 33.0 40.0 44-45 37.13237006992419 40.0 37.0 40.0 33.0 40.0 46-47 37.058214984022555 38.5 37.0 40.0 33.0 40.0 48-49 36.84933289191433 37.0 37.0 40.0 33.0 40.0 50-51 36.661402770980565 37.0 37.0 40.0 33.0 40.0 52-53 36.55033285378942 37.0 37.0 40.0 33.0 40.0 54-55 36.40846462436461 37.0 37.0 40.0 33.0 40.0 56-57 36.21996185175013 37.0 37.0 40.0 33.0 40.0 58-59 35.9606523716416 37.0 37.0 40.0 33.0 40.0 60-61 35.70775662926017 37.0 37.0 40.0 27.0 40.0 62-63 35.48026180140969 37.0 33.0 40.0 27.0 40.0 64-65 35.168765741113205 37.0 33.0 37.0 27.0 40.0 66-67 34.84441925261175 37.0 33.0 37.0 27.0 40.0 68-69 34.56707474582746 37.0 33.0 37.0 27.0 40.0 70-71 34.25630928325951 37.0 33.0 37.0 27.0 40.0 72-73 33.90917343643139 37.0 33.0 37.0 27.0 37.0 74-75 33.510373865297694 37.0 33.0 37.0 27.0 37.0 76-77 30.823818575233283 33.0 30.0 35.0 24.5 37.0 78-79 33.257126634605434 35.0 33.0 37.0 27.0 37.0 80-81 33.69967765001179 37.0 33.0 37.0 27.0 37.0 82-83 33.67493983656085 37.0 33.0 37.0 27.0 37.0 84-85 33.56716947455318 37.0 33.0 37.0 27.0 37.0 86-87 33.43643877567693 37.0 33.0 37.0 27.0 37.0 88-89 33.32730914398689 37.0 33.0 37.0 27.0 37.0 90-91 33.198333552486716 37.0 33.0 37.0 27.0 37.0 92-93 33.04804224706652 37.0 33.0 37.0 27.0 37.0 94-95 32.973954268783714 33.0 33.0 37.0 27.0 37.0 96-97 32.84504286717982 33.0 33.0 37.0 27.0 37.0 98-99 32.554193778793056 33.0 33.0 37.0 27.0 37.0 100-101 30.988335334242628 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17866254635392664 2306 2 0.16847497089854357 2306 3 0.17354069913683645 2306 4 0.2152864978233211 2306 5 0.22111726238914287 2306 6 0.2477675432796218 2306 7 0.23163640004631247 2306 8 0.21466802474856905 2306 9 0.19908679662139406 2306 10-11 0.26257627743620304 2306 12-13 0.2923985956331947 2306 14-15 0.47186230067974577 2306 16-17 0.5182562372280728 2306 18-19 0.6728060051538236 2306 20-21 0.60823346698335 2306 22-23 0.6826827456488473 2306 24-25 0.6226001701507329 2306 26-27 0.6765763242427667 2306 28-29 0.7279378631428486 2306 30-31 0.8133977912938732 2306 32-33 0.6670058324522401 2306 34-35 0.7825668475375451 2306 36-37 0.6907756949982655 2306 38-39 0.8423986681386495 2306 40-41 0.788238643351427 2306 42-43 0.8528270199345442 2306 44-45 0.8823939775426481 2306 46-47 0.8196135856429265 2306 48-49 0.935743341425237 2306 50-51 0.9537198673630058 2306 52-53 0.8930575488936299 2306 54-55 0.824734462999686 2306 56-57 0.8623559705086947 2306 58-59 0.8375000058339737 2306 60-61 0.7659192056341979 2306 62-63 0.925542229364531 2306 64-65 0.8674158297089392 2306 66-67 0.9373830566601811 2306 68-69 0.9671030585935441 2306 70-71 0.9667266541956465 2306 72-73 0.8858643427155357 2306 74-75 0.8480354020499448 2306 76-77 0.5480770834063584 2306 78-79 0.8381324974364404 2306 80-81 0.893258835925387 2306 82-83 0.9644736965579384 2306 84-85 0.9911414102671117 2306 86-87 0.9173117018336185 2306 88-89 0.95670440986882 2306 90-91 0.9888433802581389 2306 92-93 0.9693942677700278 2306 94-95 1.0489452298171358 2306 96-97 1.0096600179181507 2306 98-99 1.0243235493545484 2306 100-101 0.892456222144542 2307 1 0.11734041734861478 2307 2 0.10137644352525399 2307 3 0.16171537529032065 2307 4 0.20503163595927276 2307 5 0.2277990781647361 2307 6 0.20624303838688007 2307 7 0.20389980632149474 2307 8 0.21712351362103277 2307 9 0.27178134470674564 2307 10-11 0.283630467197419 2307 12-13 0.2678454714725902 2307 14-15 0.5358010820718278 2307 16-17 0.5680863489249788 2307 18-19 0.53735787451334 2307 20-21 0.5017857500351042 2307 22-23 0.5455314185986708 2307 24-25 0.5690617291234403 2307 26-27 0.5943147021378792 2307 28-29 0.5985837523948945 2307 30-31 0.6294957128335099 2307 32-33 0.6689472450053486 2307 34-35 0.6762751964741938 2307 36-37 0.7338594863013199 2307 38-39 0.7398286786672159 2307 40-41 0.6945349999188366 2307 42-43 0.6066664171594738 2307 44-45 0.740060125950329 2307 46-47 0.7963442798420175 2307 48-49 0.8007069423906472 2307 50-51 0.794436005869521 2307 52-53 0.7599109979300849 2307 54-55 0.6098611488935646 2307 56-57 0.6746210629228315 2307 58-59 0.6627085674986191 2307 60-61 0.6246231137513831 2307 62-63 0.5747512145245537 2307 64-65 0.7578322864251632 2307 66-67 0.5447742271739244 2307 68-69 0.5134748208636353 2307 70-71 0.507777471678942 2307 72-73 0.5277511714214569 2307 74-75 0.4829972972701242 2307 76-77 0.19692433294119738 2307 78-79 0.5009923459584229 2307 80-81 0.5013081888205448 2307 82-83 0.5412618371191869 2307 84-85 0.5436493459036598 2307 86-87 0.644901444619002 2307 88-89 0.7461830988369655 2307 90-91 0.8072099429462369 2307 92-93 0.8195308254193208 2307 94-95 0.6551925707171833 2307 96-97 0.6786064359512167 2307 98-99 0.6784390028180951 2307 100-101 0.40884882587639737 2201 1 0.09377681643741909 2201 2 0.12155493292744524 2201 3 0.12984726745922615 2201 4 0.15738633989872852 2201 5 0.18896075918992494 2201 6 0.17531143745068078 2201 7 0.17294445679537063 2201 8 0.2652359694439639 2201 9 0.2066561490152381 2201 10-11 0.23919482431370653 2201 12-13 0.26820841867260015 2201 14-15 0.5255474721299223 2201 16-17 0.4747017373862903 2201 18-19 0.527832346341583 2201 20-21 0.44874530556149494 2201 22-23 0.42373146297103403 2201 24-25 0.5130281811153523 2201 26-27 0.47671918437174554 2201 28-29 0.474589083328361 2201 30-31 0.48321033602776 2201 32-33 0.45674015730117645 2201 34-35 0.6192884238138205 2201 36-37 0.6609185046721038 2201 38-39 0.6294785119583608 2201 40-41 0.6032983858427983 2201 42-43 0.6930784739436007 2201 44-45 0.6628222119514646 2201 46-47 0.7236648451834498 2201 48-49 0.8714743030786138 2201 50-51 0.7887182203630303 2201 52-53 0.7875587313459178 2201 54-55 0.7891826891771103 2201 56-57 0.7589723045678625 2201 58-59 0.724132889179522 2201 60-61 0.7849665621091404 2201 62-63 0.835599632899708 2201 64-65 0.7441077888172103 2201 66-67 0.8837951915541638 2201 68-69 1.0748282092562036 2201 70-71 0.8443577997219691 2201 72-73 0.7548067524963358 2201 74-75 0.6494181972791893 2201 76-77 0.46505278781418724 2201 78-79 0.6375450193934356 2201 80-81 0.7189307362317976 2201 82-83 0.6906626539456937 2201 84-85 0.6253419954025929 2201 86-87 0.7402781253785875 2201 88-89 0.9072149438211667 2201 90-91 0.9196915456010757 2201 92-93 0.9385456339150622 2201 94-95 0.8902200222122474 2201 96-97 0.9415309810563528 2201 98-99 1.0656135143314671 2201 100-101 0.9423059018625075 1101 1 0.08747578384387822 1101 2 0.10362480049516165 1101 3 0.09353391384375698 1101 4 0.10252893996562307 1101 5 0.15252344733129064 1101 6 0.13552458151260538 1101 7 0.14584568879070048 1101 8 0.16378469625389158 1101 9 0.17963271770145894 1101 10-11 0.12908971627234678 1101 12-13 0.1855330792033314 1101 14-15 0.3821138929104535 1101 16-17 0.3979942927320437 1101 18-19 0.3876136669029435 1101 20-21 0.40480424556890426 1101 22-23 0.375275277401272 1101 24-25 0.38705294498024756 1101 26-27 0.41256171038428135 1101 28-29 0.34660673882917337 1101 30-31 0.4872392460821615 1101 32-33 0.4539804834823329 1101 34-35 0.3656353957597176 1101 36-37 0.3181648791606406 1101 38-39 0.42408451175105455 1101 40-41 0.46551666134853775 1101 42-43 0.47123088701521 1101 44-45 0.5411398314402689 1101 46-47 0.500574737158118 1101 48-49 0.4992952881405728 1101 50-51 0.43172515881882845 1101 52-53 0.4646787646009116 1101 54-55 0.401538013343945 1101 56-57 0.42730252291602966 1101 58-59 0.5521603288326986 1101 60-61 0.43331278816836516 1101 62-63 0.4931227762242898 1101 64-65 0.44728550112508714 1101 66-67 0.4991971101285557 1101 68-69 0.6602720385400502 1101 70-71 0.5387446285174704 1101 72-73 0.4403562134382639 1101 74-75 0.43040027783280976 1101 76-77 0.37578628934603486 1101 78-79 0.648331523760298 1101 80-81 0.5357111025284027 1101 82-83 0.48126645882259567 1101 84-85 0.5181186493635366 1101 86-87 0.47940987268756885 1101 88-89 0.5961208947785792 1101 90-91 0.41865766973671015 1101 92-93 0.4735381910774379 1101 94-95 0.5234398696367464 1101 96-97 0.5416269952645294 1101 98-99 0.491666812101613 1101 100-101 0.46792785700458595 2304 1 0.08857636848755845 2304 2 0.08626606454556196 2304 3 0.10705885968386752 2304 4 0.1424771074213993 2304 5 0.17851820843910104 2304 6 0.1720423326935432 2304 7 0.1489969226824499 2304 8 0.1789199084548585 2304 9 0.1538962748128867 2304 10-11 0.170914913326925 2304 12-13 0.18299100438145643 2304 14-15 0.31202831009735377 2304 16-17 0.3657690948826442 2304 18-19 0.4072035022797209 2304 20-21 0.4221827279593029 2304 22-23 0.4417856367070101 2304 24-25 0.43737656306470996 2304 26-27 0.40985433674038063 2304 28-29 0.4217070482645937 2304 30-31 0.4825734974387075 2304 32-33 0.4476449807727292 2304 34-35 0.5108492310848334 2304 36-37 0.4910932788146951 2304 38-39 0.5078155061122658 2304 40-41 0.45634127271623726 2304 42-43 0.47357102653685956 2304 44-45 0.39800309814241075 2304 46-47 0.3878931009506488 2304 48-49 0.4392803901340443 2304 50-51 0.3867801483097466 2304 52-53 0.5007418508777093 2304 54-55 0.5996362944845828 2304 56-57 0.41969914967749844 2304 58-59 0.43027834151115485 2304 60-61 0.5457952212875412 2304 62-63 0.5105223711614499 2304 64-65 0.41828033923159325 2304 66-67 0.2500987400668393 2304 68-69 0.38980416960078657 2304 70-71 0.4436301215153051 2304 72-73 0.3870721195316449 2304 74-75 0.39899524032947653 2304 76-77 0.3091282664043966 2304 78-79 0.4971076915200925 2304 80-81 0.47724648641163014 2304 82-83 0.4913481966683477 2304 84-85 0.4918638313561772 2304 86-87 0.5381924189203247 2304 88-89 0.5848941117494917 2304 90-91 0.6066357136816052 2304 92-93 0.4640047584903044 2304 94-95 0.5677333717909718 2304 96-97 0.4429732802759574 2304 98-99 0.5334367439602445 2304 100-101 0.5767605517454761 2202 1 0.005802361109303433 2202 2 0.02784842249293007 2202 3 0.07544961253082505 2202 4 0.12326469377924809 2202 5 0.10992187299960676 2202 6 0.06335185040102687 2202 7 0.17843513084452667 2202 8 0.0498868162146735 2202 9 0.05022483384608023 2202 10-11 0.11327039943139283 2202 12-13 0.1732552694444749 2202 14-15 0.15060868656470916 2202 16-17 0.20626687997494741 2202 18-19 0.1620128973857291 2202 20-21 0.17521636417139774 2202 22-23 0.2487304447999108 2202 24-25 0.24962209573003236 2202 26-27 0.12622147252510274 2202 28-29 0.17047179796131928 2202 30-31 0.2051021079137243 2202 32-33 0.22356630433303337 2202 34-35 0.23172133626997748 2202 36-37 0.27285872163691494 2202 38-39 0.28104098246865306 2202 40-41 0.30143846036299493 2202 42-43 0.44360916460135513 2202 44-45 0.3155307458148542 2202 46-47 0.20302577145098155 2202 48-49 0.22849600850034335 2202 50-51 0.30795097626916856 2202 52-53 0.31572109636373824 2202 54-55 0.43219326723011164 2202 56-57 0.58206829972832 2202 58-59 0.4783389292686451 2202 60-61 0.4974188943170148 2202 62-63 0.47110703475546956 2202 64-65 0.37340658181258135 2202 66-67 0.3400473582719812 2202 68-69 0.37203757603322885 2202 70-71 0.3881366748067734 2202 72-73 0.4210981858528129 2202 74-75 0.3819543274541104 2202 76-77 0.32992757263241046 2202 78-79 0.5243986421456341 2202 80-81 0.54870941066239 2202 82-83 0.6546099593682229 2202 84-85 0.5107508084931993 2202 86-87 0.39944633420200404 2202 88-89 0.43607115521136564 2202 90-91 0.5328068810462483 2202 92-93 0.3543041416039898 2202 94-95 0.6308786746304733 2202 96-97 0.563083478456285 2202 98-99 0.6012601821305381 2202 100-101 0.5374075550744166 1102 1 0.10224390054640509 1102 2 0.0726417496477012 1102 3 0.07978442546678366 1102 4 0.10923786581963668 1102 5 0.12737741175381245 1102 6 0.12223014902060214 1102 7 0.10110895163375488 1102 8 0.1738397941181944 1102 9 0.13669710349146413 1102 10-11 0.07689178375411387 1102 12-13 0.053273122232141645 1102 14-15 0.16203339755441704 1102 16-17 0.2281274721411819 1102 18-19 0.18125537336251085 1102 20-21 0.22615524520158914 1102 22-23 0.22493482507403684 1102 24-25 0.23687022996738705 1102 26-27 0.2546522807783589 1102 28-29 0.331664617823769 1102 30-31 0.2380290870854722 1102 32-33 0.20199496634952396 1102 34-35 0.15696078800403512 1102 36-37 0.19809456379096702 1102 38-39 0.2574533525969329 1102 40-41 0.23409062367209543 1102 42-43 0.13610143327117896 1102 44-45 0.2970845236793451 1102 46-47 0.3068466582369922 1102 48-49 0.2685528837747171 1102 50-51 0.18477327762031592 1102 52-53 0.11908239581254776 1102 54-55 0.15089352575408554 1102 56-57 0.31406927529048545 1102 58-59 0.20301177449570673 1102 60-61 0.017556058357790505 1102 62-63 0.3501098151076434 1102 64-65 0.28297821246474797 1102 66-67 0.23392629100697349 1102 68-69 0.10999591952866439 1102 70-71 0.3047711804406461 1102 72-73 0.263600795481608 1102 74-75 0.26175316653512937 1102 76-77 0.09250266653027239 1102 78-79 0.447363375573282 1102 80-81 0.43238445090432975 1102 82-83 0.46710378893751425 1102 84-85 0.44427785595429725 1102 86-87 0.4193485828728072 1102 88-89 0.3965989343440981 1102 90-91 0.5585918671444787 1102 92-93 0.5456231992109366 1102 94-95 0.43165658790299943 1102 96-97 0.43983153952648024 1102 98-99 0.3460253155386681 1102 100-101 0.3054619914433765 2305 1 0.0751794767191285 2305 2 0.06530072823089483 2305 3 0.06923695299647648 2305 4 0.0322077782448531 2305 5 0.08183436137663591 2305 6 0.027145435589581268 2305 7 0.00414476191728852 2305 8 0.11882115196090126 2305 9 -0.07637356329081513 2305 10-11 -0.015466319081227198 2305 12-13 0.06526106986068925 2305 14-15 0.052515579120353095 2305 16-17 0.010943131553304397 2305 18-19 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-0.37741516527425034 1213 64-65 -0.6316250338146929 1213 66-67 -0.5314748681818671 1213 68-69 -0.10936347031397275 1213 70-71 0.03354456039851783 1213 72-73 0.028737681641558765 1213 74-75 0.12740604316825 1213 76-77 -2.275424478037923 1213 78-79 -1.8609078911365273 1213 80-81 -1.3583580632413614 1213 82-83 -0.9292056952096814 1213 84-85 -0.5813957814481299 1213 86-87 -0.4888275807903426 1213 88-89 -0.3354571803082109 1213 90-91 -1.632191287446151 1213 92-93 -0.7588054528534371 1213 94-95 -0.3912983248824915 1213 96-97 -0.4218511290859368 1213 98-99 -2.4377854145300333 1213 100-101 -0.8302003844807437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 50.0 9 143.0 10 104.0 11 27.0 12 44.0 13 100.0 14 326.0 15 749.0 16 1535.0 17 2415.0 18 3089.0 19 3716.0 20 4460.0 21 5566.0 22 6933.0 23 8815.0 24 10835.0 25 13938.0 26 17526.0 27 23149.0 28 30999.0 29 39201.0 30 44826.0 31 56726.0 32 76923.0 33 110013.0 34 171598.0 35 301766.0 36 585350.0 37 856698.0 38 192793.0 39 84.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.92278075333113 24.293791035097673 25.112817291325033 13.670610920246165 2 32.491985433720316 20.959141274238227 33.6896962245946 12.859177067446856 3 38.59362806161392 17.392425785599297 27.957430154470526 16.056515998316254 4 31.68911238211428 17.077974773759795 34.74408460928583 16.488828234840096 5 30.471906650389464 16.071054820409998 37.30160212194987 16.15543640725067 6 25.400330986446985 16.808727336103743 41.63088242044927 16.160059257000007 7 34.34116657550405 17.830863902636764 30.160575888407614 17.66739363345157 8 30.79395510130722 27.683857369272413 24.451370901669637 17.07081662775073 9 29.223986947276366 26.524899066250974 25.37010338074568 18.881010605726985 10-11 24.657944860361365 27.470441456343764 30.080078055096067 17.791535628198805 12-13 24.336587696236293 25.994651620036276 29.622742363542002 20.04601832018543 14-15 23.220578269269225 26.920931274795777 30.70138549028243 19.157104965652568 16-17 22.277220211803318 27.893836136032785 31.533539415319524 18.29540423684438 18-19 20.981059304873725 28.112831876481476 31.86202901617858 19.044079802466214 20-21 21.544832946767514 28.075065609854587 31.514690927594575 18.865410515783324 22-23 20.854538692502388 28.27319842302931 31.76598464577681 19.10627823869149 24-25 21.538744632363066 26.998464888904795 31.949451040226446 19.51333943850569 26-27 21.366462841052588 27.40299739583536 31.611131383879698 19.61940837923235 28-29 21.115849924800564 28.658765733332608 30.89068732984815 19.334697012018683 30-31 20.157475321902606 28.915797639212325 30.87808276839741 20.048644270487664 32-33 19.639423955980515 29.82124475490742 31.320817989354595 19.21851329975748 34-35 20.173036508878823 29.434199131841375 31.633714556478935 18.75904980280086 36-37 20.47994201901733 30.217490929773138 31.320195541875545 17.982371509333987 38-39 21.033838308586418 30.278421732207374 30.298651346105743 18.389088613100466 40-41 21.170279294183324 29.57968253227245 29.80668139144271 19.44335678210152 42-43 20.431802341877916 29.712170027253894 30.922985140588732 18.93304249027946 44-45 19.841099407697023 29.246209358192555 31.800134216438597 19.11255701767183 46-47 20.53383588672396 29.01961821789759 31.170597825463204 19.27594806991525 48-49 20.549696718112994 30.676677893277297 29.721566569314046 19.052058819295663 50-51 20.816133427435137 29.718752845036274 29.704921591772994 19.7601921357556 52-53 19.959465168990445 29.46221612196164 29.78220746955122 20.7961112394967 54-55 21.163181484164195 29.315767243078323 29.969106420879925 19.551944851877558 56-57 21.04243983376336 29.45338897899242 29.254084763722858 20.25008642352136 58-59 20.55585160351312 29.535813015461486 30.261386675662894 19.6469487053625 60-61 20.440855487641187 29.803441715332866 30.346817210911087 19.40888558611486 62-63 20.36356583824145 29.823152266927234 29.86520931160866 19.94807258322266 64-65 20.932744858219117 29.657581642751353 29.55803474939454 19.851638749634994 66-67 21.11999118953703 29.988516044070995 29.098635934858613 19.792856831533364 68-69 21.934637140005176 30.09557703271451 28.59379955674984 19.37598627053048 70-71 20.99058682739598 31.086975768985663 28.72896386995714 19.193473533661212 72-73 20.243525338457722 31.230690621484232 28.852818123409573 19.672965916648476 74-75 21.072251728630125 30.190039860405882 28.685910314573228 20.05179809639077 76-77 21.29157983425468 29.30275275771957 29.099414112285743 20.306253295740007 78-79 21.10632323950389 29.258350711497954 29.640115866103795 19.99521018289436 80-81 19.724757257757673 29.851440736190398 29.995288711347783 20.428513294704135 82-83 19.08231401074811 29.690939265465293 30.10278553027468 21.12396119351192 84-85 19.07442137962387 29.228462720447073 30.613953573421792 21.08316232650726 86-87 19.207539401806983 29.29542152209155 31.574056563024598 19.92298251307686 88-89 19.553644705029907 29.925160588364168 30.972783935054082 19.54841077155184 90-91 19.969815047609302 29.73044646840532 29.992487810933323 20.30725067305206 92-93 19.144935587534505 30.631768514023243 30.14230966435078 20.08098623409147 94-95 18.906579506012662 30.90565253549009 30.35775925306879 19.830008705428458 96-97 19.528817123790386 31.59179691300419 29.410013472819696 19.46937249038573 98-99 20.01213091109486 30.6667836817646 28.831054198357712 20.490031208782828 100-101 19.506672667193172 30.719621507645904 28.56344009951068 21.210265725650245 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1496.0 1 901.5 2 378.0 3 631.0 4 687.0 5 491.0 6 439.5 7 463.5 8 493.0 9 508.0 10 486.0 11 465.5 12 437.0 13 438.5 14 440.0 15 431.0 16 447.5 17 457.0 18 491.0 19 540.0 20 620.5 21 723.5 22 901.0 23 1201.0 24 1583.0 25 2167.5 26 3364.0 27 5258.5 28 7212.5 29 9097.5 30 11387.0 31 14697.0 32 22118.5 33 33808.0 34 49499.0 35 75584.0 36 121090.0 37 180694.5 38 228269.5 39 234858.5 40 214789.5 41 189736.0 42 164773.0 43 152687.0 44 136479.5 45 116804.5 46 111797.0 47 100616.5 48 76363.0 49 58120.5 50 47015.0 51 39357.0 52 33753.0 53 27852.5 54 22058.0 55 16148.0 56 11310.5 57 8414.5 58 6713.0 59 5235.0 60 4053.0 61 3233.5 62 2416.0 63 1737.5 64 1280.5 65 893.5 66 615.5 67 446.0 68 339.5 69 260.0 70 200.5 71 145.0 72 95.0 73 68.0 74 53.0 75 35.5 76 19.5 77 16.0 78 15.0 79 13.0 80 7.5 81 3.0 82 3.5 83 5.0 84 3.0 85 2.0 86 2.5 87 1.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.171250862673303 2 0.006924728204417977 3 0.001556118697622017 4 0.0 5 0.0012448949580976137 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.334178046433026E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.890296744055043E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.501267069649539E-4 40-41 1.9451483720275214E-5 42-43 0.0015366672139017418 44-45 8.947682511326599E-4 46-47 0.006127217371886693 48-49 0.0045516471905444 50-51 0.009025488446207701 52-53 0.0045127442231038505 54-55 0.02126047170626081 56-57 0.011923759520528708 58-59 0.020676927194652552 60-61 0.010425995274067516 62-63 0.006983082655578802 64-65 0.013713296022794029 66-67 0.03213385110589466 68-69 0.032795201552384017 70-71 0.03736630022664869 72-73 0.032172754073335204 74-75 0.03522663701741842 76-77 0.03474034992441153 78-79 0.017992622441254574 80-81 0.0029566255254818326 82-83 0.0 84-85 0.007060888590459903 86-87 0.0269208534688609 88-89 0.028360263264161262 90-91 0.026240051538651263 92-93 0.036004696366229424 94-95 0.03256178374774071 96-97 0.03423461134768438 98-99 0.040031153496326397 100-101 0.028146296943238235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2570498.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.44678331292171 #Duplication Level Percentage of deduplicated Percentage of total 1 85.52954862341072 32.883360227762736 2 6.117118497411088 4.703670587388587 3 2.2254171475448143 2.566803925575474 4 1.3444872450896548 2.067648391157961 5 0.8363780573263421 1.6078022968854147 6 0.5282088592915407 1.2184758934288453 7 0.4574569235672747 1.231142305077076 8 0.3167852714803136 0.9743499751462955 9 0.27912122142220513 0.9658181806251807 >10 1.9065437426255711 14.513361873728542 >50 0.21905475095614324 5.781834124096906 >100 0.20227183515431393 17.899807474841584 >500 0.03004555943045768 7.530589904256227 >1k 0.007156359241390898 4.7158125534461295 >5k 3.0442953618090306E-4 0.6181450203468597 >10k+ 1.0147651188228183E-4 0.7213772662361357 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 18490 0.7193158679757774 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5392 0.20976480043944792 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5245 0.204046064225687 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5207 0.20256775146294612 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4981 0.1937756808213817 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4947 0.19245297992840296 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4124 0.16043583772482997 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 3944 0.1534333035855309 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3596 0.13989507091621936 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3532 0.1374052810000241 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3340 0.12993591125143844 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3064 0.11919869223784652 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2959 0.11511388065658873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.9451483720275215E-4 0.0 0.0 2 2.334178046433026E-4 0.0 0.0 3 3.112237395244034E-4 0.0 0.0 4 3.112237395244034E-4 0.0 0.0 5 3.501267069649539E-4 0.0 0.0 6 4.279326418460547E-4 0.0 0.0 7 5.44641544167706E-4 0.0 0.0 8 6.613504464893573E-4 0.0 0.0 9 8.169623162515591E-4 0.0 0.0 10-11 0.0013810553441395402 0.0 0.0 12-13 0.0021202117255099988 0.0 0.0 14-15 0.0030344314603629337 0.0 0.0 16-17 0.004765613511467428 0.0 0.0 18-19 0.006808019302096326 0.0 0.0 20-21 0.008325235032277792 0.0 0.0 22-23 0.00988135372989981 0.0 0.0 24-25 0.01134021500892045 0.0 0.0 26-27 0.01272127035305999 0.0 0.0 28-29 0.014588612790206412 0.0 0.0 30-31 0.02190237066902989 0.0 0.0 32-33 0.024119839813141267 0.0 0.0 34-35 0.026064988185168787 0.0 0.0 36-37 0.02853532661764374 0.0 0.0 38-39 0.030636086859433462 0.0 0.0 40-41 0.033398197547712546 0.0 0.0 42-43 0.036140856752271355 0.0 0.0 44-45 0.03936980304983703 0.0 0.0 46-47 0.042637652314843275 0.0 0.0 48-49 0.04635288570541583 0.0 3.890296744055043E-5 50-51 0.0496790894215829 0.0 3.890296744055043E-5 52-53 0.0536860950679596 0.0 3.890296744055043E-5 54-55 0.05812103335618234 0.0 3.890296744055043E-5 56-57 0.06366470621646078 0.0 3.890296744055043E-5 58-59 0.0694612483651028 0.0 3.890296744055043E-5 60-61 0.0756662716718706 0.0 3.890296744055043E-5 62-63 0.08385534631810646 0.0 3.890296744055043E-5 64-65 0.09235564470386673 0.0 3.890296744055043E-5 66-67 0.1021008380477246 0.0 3.890296744055043E-5 68-69 0.11303257189851927 0.0 3.890296744055043E-5 70-71 0.12583164818646037 0.0 3.890296744055043E-5 72-73 0.14065367878131008 0.0 3.890296744055043E-5 74-75 0.1580433052272361 0.0 3.890296744055043E-5 76-77 0.17796162455679795 0.0 3.890296744055043E-5 78-79 0.2014590168908904 0.0 3.890296744055043E-5 80-81 0.22802974365278633 0.0 3.890296744055043E-5 82-83 0.2586463790284995 0.0 3.890296744055043E-5 84-85 0.295079008036575 0.0 3.890296744055043E-5 86-87 0.33721092177469114 0.0 3.890296744055043E-5 88-89 0.3878236824148472 0.0 3.890296744055043E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGGA 35 3.4225577E-6 67.842636 2 ACGATCG 35 2.7632253E-4 54.386253 1 CGGAAGA 3480 0.0 54.038067 4 TCGGAAG 3545 0.0 53.048275 3 GATCGGA 3780 0.0 50.231754 1 GAAGAGC 3795 0.0 49.55173 6 ATCGGAA 3920 0.0 48.21673 2 CGGTGCG 50 2.7972206E-5 47.587975 1 AGCGCGG 55 4.9111128E-5 43.26179 1 CTCGGGT 45 9.5400267E-4 42.30042 1 CGACTGG 45 9.5400267E-4 42.30042 1 GCCGTCT 2205 0.0 41.8991 50-51 TCGTATG 2245 0.0 41.67382 44-45 TATGCCG 2205 0.0 41.46665 48-49 GGAAGAG 4640 0.0 41.244965 5 CTCGTAT 2185 0.0 41.18805 44-45 TTGTGTT 27615 0.0 41.065414 1 ATGCCGT 2250 0.0 41.059525 48-49 CGTATGC 2240 0.0 40.920376 46-47 CGTCTTC 2270 0.0 40.701324 52-53 >>END_MODULE