FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34311-1.34100000035764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34311-1.34100000035764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570498
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT184900.7193158679757774TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG53920.20976480043944792No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC52450.204046064225687No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52070.20256775146294612No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG49810.1937756808213817No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC49470.19245297992840296No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT41240.16043583772482997No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT39440.1534333035855309No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA35960.13989507091621936No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA35320.1374052810000241No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT33400.12993591125143844No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG30640.11919869223784652No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT29590.11511388065658873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGA353.4225577E-667.8426362
ACGATCG352.7632253E-454.3862531
CGGAAGA34800.054.0380674
TCGGAAG35450.053.0482753
GATCGGA37800.050.2317541
GAAGAGC37950.049.551736
ATCGGAA39200.048.216732
CGGTGCG502.7972206E-547.5879751
AGCGCGG554.9111128E-543.261791
CTCGGGT459.5400267E-442.300421
CGACTGG459.5400267E-442.300421
GCCGTCT22050.041.899150-51
TCGTATG22450.041.6738244-45
TATGCCG22050.041.4666548-49
GGAAGAG46400.041.2449655
CTCGTAT21850.041.1880544-45
TTGTGTT276150.041.0654141
ATGCCGT22500.041.05952548-49
CGTATGC22400.040.92037646-47
CGTCTTC22700.040.70132452-53
Produced by FastQC (version 0.11.2)