##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34310-1.34100000035813.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5656279 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67928261671675 33.0 33.0 33.0 33.0 33.0 2 32.70707208749781 33.0 33.0 33.0 33.0 33.0 3 32.73614897709254 33.0 33.0 33.0 33.0 33.0 4 36.67439000091756 37.0 37.0 37.0 37.0 37.0 5 36.64244426415316 37.0 37.0 37.0 37.0 37.0 6 36.51223923006627 37.0 37.0 37.0 37.0 37.0 7 36.46898234687504 37.0 37.0 37.0 37.0 37.0 8 36.38985541554793 37.0 37.0 37.0 37.0 37.0 9 36.40063759231113 37.0 37.0 37.0 37.0 37.0 10-11 36.34380004946715 37.0 37.0 37.0 37.0 37.0 12-13 36.31704456233506 37.0 37.0 37.0 37.0 37.0 14-15 38.10231664668592 40.0 37.0 40.0 37.0 40.0 16-17 38.05238991923842 40.0 37.0 40.0 37.0 40.0 18-19 38.08620384531951 40.0 37.0 40.0 37.0 40.0 20-21 38.003512998563195 40.0 37.0 40.0 33.0 40.0 22-23 37.911772651242984 40.0 37.0 40.0 33.0 40.0 24-25 37.87984671548203 40.0 37.0 40.0 33.0 40.0 26-27 37.75942187434531 40.0 37.0 40.0 33.0 40.0 28-29 37.650991137459805 40.0 37.0 40.0 33.0 40.0 30-31 37.600180259849274 40.0 37.0 40.0 33.0 40.0 32-33 37.48477523827944 40.0 37.0 40.0 33.0 40.0 34-35 37.37452784772463 40.0 37.0 40.0 33.0 40.0 36-37 37.251360037225886 40.0 37.0 40.0 33.0 40.0 38-39 37.14918067160407 40.0 37.0 40.0 33.0 40.0 40-41 37.051024799165674 37.0 37.0 40.0 33.0 40.0 42-43 36.915651172793986 37.0 37.0 40.0 33.0 40.0 44-45 36.791837708147 37.0 37.0 40.0 33.0 40.0 46-47 36.66668829454841 37.0 37.0 40.0 33.0 40.0 48-49 36.43762860707543 37.0 37.0 40.0 33.0 40.0 50-51 36.24345590095538 37.0 37.0 40.0 33.0 40.0 52-53 36.093125091601735 37.0 37.0 40.0 33.0 40.0 54-55 35.91516215872661 37.0 37.0 40.0 33.0 40.0 56-57 35.68484342798507 37.0 35.0 40.0 27.0 40.0 58-59 35.42925393178095 37.0 33.0 40.0 27.0 40.0 60-61 35.15965425326438 37.0 33.0 40.0 27.0 40.0 62-63 34.91526355047196 37.0 33.0 37.0 27.0 40.0 64-65 34.59871339444182 37.0 33.0 37.0 27.0 40.0 66-67 34.218938104007954 37.0 33.0 37.0 27.0 40.0 68-69 33.91050264670466 37.0 33.0 37.0 27.0 38.5 70-71 33.61393161475945 37.0 33.0 37.0 27.0 37.0 72-73 33.24150046346723 37.0 33.0 37.0 27.0 37.0 74-75 32.79487406826997 35.0 33.0 37.0 27.0 37.0 76-77 30.07141161176809 33.0 30.0 33.0 22.0 37.0 78-79 32.61335915360611 33.0 33.0 37.0 27.0 37.0 80-81 33.13118447657904 37.0 33.0 37.0 27.0 37.0 82-83 33.060915930773575 37.0 33.0 37.0 27.0 37.0 84-85 32.93117091642757 35.0 33.0 37.0 27.0 37.0 86-87 32.752247723282395 33.0 33.0 37.0 27.0 37.0 88-89 32.63026178517715 33.0 33.0 37.0 27.0 37.0 90-91 32.4847170728318 33.0 33.0 37.0 24.5 37.0 92-93 32.27879362032884 33.0 33.0 37.0 22.0 37.0 94-95 32.14914292594124 33.0 33.0 37.0 22.0 37.0 96-97 32.007886987187156 33.0 33.0 37.0 22.0 37.0 98-99 31.685879798362137 33.0 33.0 37.0 22.0 37.0 100-101 29.979794048348747 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14167756785884933 2306 2 0.1723219796773776 2306 3 0.15408185967847743 2306 4 0.18321516524927972 2306 5 0.18256176766210075 2306 6 0.22241895975322024 2306 7 0.25480238490783336 2306 8 0.23177718375180234 2306 9 0.19140035357175122 2306 10-11 0.2256945593129842 2306 12-13 0.269578472600692 2306 14-15 0.5461019822281585 2306 16-17 0.552677868809134 2306 18-19 0.5447460394697501 2306 20-21 0.6160597646818289 2306 22-23 0.6370190221285839 2306 24-25 0.596910692331015 2306 26-27 0.5896872389217549 2306 28-29 0.5920748251866002 2306 30-31 0.733451066514867 2306 32-33 0.7945412936731415 2306 34-35 0.7111526877727812 2306 36-37 0.710468500996349 2306 38-39 0.8187584897174887 2306 40-41 0.7406891530429007 2306 42-43 0.7798564004193196 2306 44-45 0.8173504031082643 2306 46-47 0.8204255755621332 2306 48-49 0.8159032307896226 2306 50-51 0.7988941711581461 2306 52-53 0.7439217358359187 2306 54-55 0.755332825539611 2306 56-57 0.8042031978515638 2306 58-59 0.7644832239392443 2306 60-61 0.7479191395646723 2306 62-63 0.8217910747931754 2306 64-65 0.7247229736014305 2306 66-67 0.7280696451171877 2306 68-69 0.701924274213539 2306 70-71 0.6440236983614014 2306 72-73 0.6395874380918443 2306 74-75 0.7223944965707716 2306 76-77 0.20052140713684352 2306 78-79 0.5002087472535095 2306 80-81 0.739500306121748 2306 82-83 0.7111012655760405 2306 84-85 0.7291758933416048 2306 86-87 0.6083707194949 2306 88-89 0.6577807458384797 2306 90-91 0.7504880977649648 2306 92-93 0.7253859060952337 2306 94-95 0.7994343427269825 2306 96-97 0.8418756375066607 2306 98-99 0.7543422058889746 2306 100-101 0.4923918813909687 2307 1 0.12731910860155438 2307 2 0.10438088605110352 2307 3 0.11644550592789926 2307 4 0.17684180401869298 2307 5 0.18363142126440124 2307 6 0.20456598530676473 2307 7 0.26618838043789594 2307 8 0.285735924107442 2307 9 0.2377580736990268 2307 10-11 0.2781746308058217 2307 12-13 0.2731515816767427 2307 14-15 0.5251343800796135 2307 16-17 0.5074991591878941 2307 18-19 0.4443641692500222 2307 20-21 0.5161206366181545 2307 22-23 0.5211998408050249 2307 24-25 0.4759975233478215 2307 26-27 0.5439191262502092 2307 28-29 0.6443795168226174 2307 30-31 0.6102957743970236 2307 32-33 0.6923352072153719 2307 34-35 0.6716172031949696 2307 36-37 0.6698693927763486 2307 38-39 0.6463745010399151 2307 40-41 0.6815065253784311 2307 42-43 0.6683349454124254 2307 44-45 0.6961619000141894 2307 46-47 0.6825495155666843 2307 48-49 0.6884769894661176 2307 50-51 0.7268817302262036 2307 52-53 0.6473244321070339 2307 54-55 0.6217972125951903 2307 56-57 0.6000299748808047 2307 58-59 0.5534511768475028 2307 60-61 0.6425363883575557 2307 62-63 0.4836337733699807 2307 64-65 0.44183894383836986 2307 66-67 0.472394226218924 2307 68-69 0.5143478521343141 2307 70-71 0.37127041039789077 2307 72-73 0.4763990363828512 2307 74-75 0.431004850359777 2307 76-77 0.11158194834517943 2307 78-79 0.4053973337450145 2307 80-81 0.5759819940527109 2307 82-83 0.5271012230597165 2307 84-85 0.4586298773873523 2307 86-87 0.4969325596997294 2307 88-89 0.5558099300277988 2307 90-91 0.6284734901354838 2307 92-93 0.6182530356175491 2307 94-95 0.5938515399279325 2307 96-97 0.6194450239510587 2307 98-99 0.6043277339847037 2307 100-101 0.32683656703381914 2201 1 0.10379345681350571 2201 2 0.09022816913145704 2201 3 0.07803262567311009 2201 4 0.15544280387359777 2201 5 0.15435167125783522 2201 6 0.1676105471897813 2201 7 0.15358880316099288 2201 8 0.24706432164929737 2201 9 0.22080616080091175 2201 10-11 0.23764352693392254 2201 12-13 0.28607244257472786 2201 14-15 0.5041073347872143 2201 16-17 0.4432955194085082 2201 18-19 0.41819927207466634 2201 20-21 0.4147431879659038 2201 22-23 0.46834847898460197 2201 24-25 0.4340690952346691 2201 26-27 0.4707545547362031 2201 28-29 0.4558746831678988 2201 30-31 0.5612669770453849 2201 32-33 0.581022268128109 2201 34-35 0.47554509373346576 2201 36-37 0.512922028586857 2201 38-39 0.5847982831583494 2201 40-41 0.48647564090804707 2201 42-43 0.5142416503192919 2201 44-45 0.5841664727335854 2201 46-47 0.6710371354333731 2201 48-49 0.6527993067633489 2201 50-51 0.5640981400412031 2201 52-53 0.557827043794596 2201 54-55 0.592723524788795 2201 56-57 0.5360017471788296 2201 58-59 0.5521838503573662 2201 60-61 0.5757695676399166 2201 62-63 0.5982832102414264 2201 64-65 0.5860064801378471 2201 66-67 0.5881253911121647 2201 68-69 0.6602077542127844 2201 70-71 0.6719774611327338 2201 72-73 0.7573957541215393 2201 74-75 0.6677907214474814 2201 76-77 0.28245419990683374 2201 78-79 0.5304677820365526 2201 80-81 0.5116191615981691 2201 82-83 0.5527172071563342 2201 84-85 0.4274075886908477 2201 86-87 0.619476843670121 2201 88-89 0.6354730854818911 2201 90-91 0.7887231852561953 2201 92-93 0.7335429115030507 2201 94-95 0.7517627610439774 2201 96-97 0.7208817719506015 2201 98-99 0.6938754479190692 2201 100-101 0.42013220586999367 1101 1 0.10076804332964429 1101 2 0.09208977453480571 1101 3 0.11852638510865177 1101 4 0.15983291772616326 1101 5 0.1607695201163537 1101 6 0.12571372125083968 1101 7 0.11105146414177369 1101 8 0.17630630592246632 1101 9 0.18945032438711706 1101 10-11 0.19392242838431173 1101 12-13 0.228776700775839 1101 14-15 0.36852597205921 1101 16-17 0.3489911363796736 1101 18-19 0.3601852780800314 1101 20-21 0.39312784545462165 1101 22-23 0.4184457429651687 1101 24-25 0.3871224592955329 1101 26-27 0.43983984938360976 1101 28-29 0.43112592968308405 1101 30-31 0.41637424881100316 1101 32-33 0.4004145633921823 1101 34-35 0.3991540921606145 1101 36-37 0.410038151165395 1101 38-39 0.40553270424225474 1101 40-41 0.3999359488365499 1101 42-43 0.5569355274074326 1101 44-45 0.48165210782215695 1101 46-47 0.5107567786134766 1101 48-49 0.5220823883736827 1101 50-51 0.5172414048583107 1101 52-53 0.48318180733441096 1101 54-55 0.46578585613876555 1101 56-57 0.5178988137009952 1101 58-59 0.6203882924668633 1101 60-61 0.4328071488056153 1101 62-63 0.504727417270395 1101 64-65 0.46563842914437004 1101 66-67 0.4523015639513872 1101 68-69 0.566949139809978 1101 70-71 0.510740123613175 1101 72-73 0.6289528505873037 1101 74-75 0.4901171516498124 1101 76-77 0.21119681502206689 1101 78-79 0.5074505388436421 1101 80-81 0.5140669103936801 1101 82-83 0.3802797403789384 1101 84-85 0.4831890339908327 1101 86-87 0.5093762645163551 1101 88-89 0.4828089628149712 1101 90-91 0.5369853291844251 1101 92-93 0.5071357659482416 1101 94-95 0.4893465339495293 1101 96-97 0.4941797529751568 1101 98-99 0.5996216191616526 1101 100-101 0.6044881600847773 2304 1 0.07758535236322217 2304 2 0.1072968738524196 2304 3 0.09949588496775164 2304 4 0.12303694446750058 2304 5 0.14229052962898692 2304 6 0.09931582543196527 2304 7 0.11573751743533478 2304 8 0.18037377153386558 2304 9 0.20853006813313613 2304 10-11 0.17248549811456115 2304 12-13 0.1975444591339155 2304 14-15 0.35526170147777236 2304 16-17 0.347461190931476 2304 18-19 0.3422081089507145 2304 20-21 0.3523536489164627 2304 22-23 0.4091573317829429 2304 24-25 0.37463513297051776 2304 26-27 0.35530992453159627 2304 28-29 0.4185249498664021 2304 30-31 0.38031402743946074 2304 32-33 0.4195343292697373 2304 34-35 0.3781065936140493 2304 36-37 0.45830257153725995 2304 38-39 0.4431495290782621 2304 40-41 0.3977605704518723 2304 42-43 0.4409274237449665 2304 44-45 0.46488058785180897 2304 46-47 0.42978046797058767 2304 48-49 0.4556414371669746 2304 50-51 0.372128949690115 2304 52-53 0.4621917440573142 2304 54-55 0.45967878312809773 2304 56-57 0.5220538130971448 2304 58-59 0.5104933002419259 2304 60-61 0.5947688511364575 2304 62-63 0.40838391878374836 2304 64-65 0.3877639039098355 2304 66-67 0.3147216016049583 2304 68-69 0.45413538736301007 2304 70-71 0.3879833523834222 2304 72-73 0.2563548338126438 2304 74-75 0.27502143556259995 2304 76-77 0.1495069823286208 2304 78-79 0.32197522571142656 2304 80-81 0.32192486974242485 2304 82-83 0.3791263306337598 2304 84-85 0.4786793823378517 2304 86-87 0.5292352227800521 2304 88-89 0.4713241323730628 2304 90-91 0.4285826709659304 2304 92-93 0.4513062265257375 2304 94-95 0.5444743285943332 2304 96-97 0.4130718842028074 2304 98-99 0.46681749075005996 2304 100-101 0.4110007672902576 2202 1 0.10020911633397844 2202 2 0.087081004194701 2202 3 0.05488813407055204 2202 4 0.11681403624515951 2202 5 0.12254288047905249 2202 6 0.10570520452274934 2202 7 0.15792966075624548 2202 8 0.13804592563638352 2202 9 0.13013551183224337 2202 10-11 0.1671804682193141 2202 12-13 0.11255870748065178 2202 14-15 0.1605063814451526 2202 16-17 0.18398039865220284 2202 18-19 0.1706355949524081 2202 20-21 0.20561521499708846 2202 22-23 0.2514817024739031 2202 24-25 0.23452014601525661 2202 26-27 0.28188567640523843 2202 28-29 0.21420306437192238 2202 30-31 0.21560296967135884 2202 32-33 0.247908034331509 2202 34-35 0.3340872121329994 2202 36-37 0.2686605093327614 2202 38-39 0.28014284820562807 2202 40-41 0.2474770444564811 2202 42-43 0.3800268875460304 2202 44-45 0.37309368751564875 2202 46-47 0.3069442502158424 2202 48-49 0.38422467175358577 2202 50-51 0.28741354367580385 2202 52-53 0.33523198186315284 2202 54-55 0.3427593163773466 2202 56-57 0.4046509112149437 2202 58-59 0.38994648073398963 2202 60-61 0.30852380330574647 2202 62-63 0.38298944046309913 2202 64-65 0.28642327942369405 2202 66-67 0.39862567403843485 2202 68-69 0.36154431576188983 2202 70-71 0.3351767416930116 2202 72-73 0.19813236804342438 2202 74-75 0.24118813867655575 2202 76-77 0.11301070249481882 2202 78-79 0.42489367871900185 2202 80-81 0.3421505716917608 2202 82-83 0.4824847786912869 2202 84-85 0.4567930826190789 2202 86-87 0.37918101803445126 2202 88-89 0.4640238677488 2202 90-91 0.4462200645271466 2202 92-93 0.38986080337272 2202 94-95 0.5594295831913598 2202 96-97 0.49950484746902646 2202 98-99 0.444716406316104 2202 100-101 0.42262931687467287 1102 1 0.06773857430160035 1102 2 0.011112697874239075 1102 3 0.05391445324166 1102 4 0.061240555107083594 1102 5 0.10092922545083383 1102 6 0.07622371749106094 1102 7 0.09402799028530495 1102 8 0.1248441267319862 1102 9 0.09078628292316893 1102 10-11 0.10591352274946075 1102 12-13 0.06461231684397006 1102 14-15 0.14768372380943617 1102 16-17 0.17858204783787102 1102 18-19 0.1796211689282572 1102 20-21 0.14635942616325082 1102 22-23 0.21351185473910306 1102 24-25 0.21569895562604557 1102 26-27 0.16566906513521218 1102 28-29 0.1814534050614185 1102 30-31 0.16787594788919336 1102 32-33 0.09494272291740202 1102 34-35 0.11323916143624757 1102 36-37 0.17755497313692103 1102 38-39 0.10308904316384115 1102 40-41 0.06220512396622979 1102 42-43 0.10270358196763141 1102 44-45 0.18140056879384048 1102 46-47 0.12990917711226757 1102 48-49 0.18432990877035849 1102 50-51 0.13327038090545074 1102 52-53 0.1080512441925876 1102 54-55 0.09594553167700326 1102 56-57 0.1478510276645295 1102 58-59 0.146919264253647 1102 60-61 -0.018940797320588842 1102 62-63 0.01270091238080795 1102 64-65 0.08278000103207006 1102 66-67 0.19748337536896088 1102 68-69 0.2002362246208662 1102 70-71 0.08963731772632855 1102 72-73 0.027639167888985128 1102 74-75 0.05753664482917742 1102 76-77 0.015086873774798448 1102 78-79 0.14547121819038722 1102 80-81 0.09706175912718606 1102 82-83 0.22526116577991928 1102 84-85 0.29678511337623803 1102 86-87 0.14896802411826826 1102 88-89 0.16037324096598837 1102 90-91 0.3002959291048839 1102 92-93 0.23216056712232813 1102 94-95 0.27785806020574455 1102 96-97 0.29134316340927313 1102 98-99 0.1001773400420447 1102 100-101 0.06446116972629667 2305 1 0.05282554210216972 2305 2 -0.011039306487177214 2305 3 0.05234684860829475 2305 4 0.03843386939336568 2305 5 0.07442458558706733 2305 6 0.027671892552149302 2305 7 -0.023900148188580772 2305 8 0.06516330438662266 2305 9 -0.031813047990141285 2305 10-11 0.017889331160887423 2305 12-13 -0.015870741958472934 2305 14-15 -0.031194194514675644 2305 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-0.048897470892704575 1213 72-73 0.054354485421050924 1213 74-75 0.10621580829396038 1213 76-77 -2.0760570696144818 1213 78-79 -1.7517241123445544 1213 80-81 -1.2513527507132238 1213 82-83 -0.8872346000780027 1213 84-85 -0.6832883899510236 1213 86-87 -0.405763020373449 1213 88-89 -0.37171615003256164 1213 90-91 -1.6074698289670657 1213 92-93 -0.7175823298526574 1213 94-95 -0.33595589035638085 1213 96-97 -0.385104866464939 1213 98-99 -2.283744030143019 1213 100-101 -0.700232777162423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 4.0 8 11.0 9 53.0 10 43.0 11 41.0 12 59.0 13 151.0 14 457.0 15 1146.0 16 2374.0 17 3928.0 18 5835.0 19 8407.0 20 11498.0 21 14959.0 22 18765.0 23 24376.0 24 31211.0 25 39406.0 26 49900.0 27 64646.0 28 85921.0 29 110687.0 30 127525.0 31 155087.0 32 209677.0 33 297737.0 34 453974.0 35 767692.0 36 1340551.0 37 1579496.0 38 250613.0 39 48.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.944583956414654 24.717231471728105 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27.19935745721081 29.223736744151672 20.20938861374717 20-21 23.350050448360133 27.849554450903145 29.00595073192111 19.79444436881561 22-23 23.227920687787854 28.464092736585307 28.434612224750584 19.873374350876254 24-25 23.47500008397747 26.78778751896786 29.46144452916838 20.27576786788629 26-27 22.646734717293825 26.684645506347906 29.311239774417068 21.357380001941205 28-29 22.394378000095116 27.89873872911856 28.323974117966955 21.38290915281937 30-31 20.961978714274878 28.456623161622684 28.81491524728536 21.766482876817072 32-33 21.00151000330783 28.07408368646596 28.843034440132815 22.081371870093395 34-35 22.007206504488195 28.10804594327826 29.0084877354883 20.87625981674525 36-37 21.325459723609814 29.321741378033156 29.308411059638324 20.044387838718706 38-39 21.23535208563388 28.552750171226034 28.84104106843631 21.37085667470377 40-41 21.730168859379084 27.79562766976744 27.782306180508037 22.691897290345437 42-43 22.365539841534996 28.438016219895985 28.338444095388045 20.857999843180977 44-45 21.275909438071935 28.60980085710649 28.26796599861392 21.846323706207656 46-47 22.048878990128408 28.37099029036266 28.436098980768314 21.14403173874062 48-49 22.125053427563273 28.2922672248917 28.722949740740955 20.859729606804077 50-51 21.76077434508068 28.31767646968858 27.601434852494965 22.32011433273577 52-53 21.098331506679294 28.42601945063888 27.84340550782603 22.632243534855796 54-55 22.05794574823994 28.458041095220636 28.294755854171104 21.18925730236832 56-57 21.9690045838914 28.229106163278477 27.555128256132118 22.246760996698008 58-59 21.34833894579053 27.910833502722355 28.173205966937797 22.567621584549315 60-61 21.106801741833486 27.819961386298882 28.943213600919908 22.13002327094772 62-63 20.811439352490684 27.912024320584898 28.83458383070782 22.441952496216594 64-65 21.92555365398635 28.413802480849387 28.27900599816499 21.38163786699927 66-67 22.159806442702013 28.70317070155678 27.74725959770825 21.38976325803295 68-69 21.757409195573054 27.918519186624362 28.650834916154682 21.6732367016479 70-71 21.355389247582632 29.28284469226653 28.411338721211738 20.9504273389391 72-73 22.342760886192917 29.56210291757486 27.679575207466588 20.415560988765634 74-75 23.37314382569806 28.496692470888014 27.429138019217696 20.701025684196228 76-77 23.413908568135035 27.20707644635864 27.861093472324516 21.51792151318181 78-79 23.071861775910143 27.36868666372959 28.620852670349418 20.938598890010848 80-81 22.230989474376518 28.474229685760605 28.200386146087546 21.09439469377533 82-83 21.346993314863006 28.422271956528313 28.5637695736013 21.666965155007382 84-85 21.544222580530807 27.943855742005084 28.75905835938064 21.752863318083463 86-87 20.75802109142142 28.561362469005115 29.242204382199183 21.438412057374283 88-89 20.748853789806113 29.125684693252822 29.118858539818582 21.006602977122483 90-91 21.427526810680277 28.22482384988707 28.397127697425706 21.950521642006947 92-93 21.334869975930786 28.558286909377316 27.645780831058598 22.4610622836333 94-95 21.08106205777031 29.611521840030974 27.167783753633266 22.139632348565442 96-97 21.10009214875131 30.39539958346041 26.420490996065134 22.084017271723155 98-99 21.335762473655304 29.00217213039008 27.275088380141476 22.38697701581314 100-101 21.97490993853131 29.06057368770922 27.03580654066166 21.92870983309781 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 195.0 1 131.5 2 137.0 3 283.5 4 284.5 5 182.5 6 171.5 7 181.5 8 187.5 9 183.5 10 173.5 11 180.0 12 164.5 13 171.0 14 190.5 15 182.0 16 191.0 17 229.0 18 245.0 19 293.5 20 359.5 21 428.0 22 555.5 23 713.0 24 996.0 25 1403.5 26 1968.5 27 2666.5 28 3578.5 29 4735.0 30 6411.0 31 9267.5 32 15231.5 33 27158.5 34 46488.0 35 79406.0 36 124731.5 37 189467.5 38 272669.0 39 325624.0 40 345679.0 41 338141.0 42 347805.5 43 391207.5 44 388926.5 45 370328.5 46 383700.0 47 368505.0 48 305268.0 49 248061.0 50 201537.0 51 166497.0 52 148810.0 53 135910.5 54 108918.5 55 69245.5 56 44090.0 57 34850.5 58 30640.5 59 26551.0 60 21572.5 61 16958.5 62 12283.5 63 8303.5 64 5785.0 65 4264.5 66 3453.5 67 2904.5 68 2321.0 69 1747.0 70 1236.5 71 887.0 72 691.0 73 509.5 74 323.0 75 200.0 76 139.5 77 101.0 78 72.5 79 37.5 80 23.5 81 17.0 82 17.0 83 12.5 84 4.5 85 6.0 86 5.0 87 5.0 88 4.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16991736086568573 2 0.007248581620531802 3 0.0020508182145894853 4 0.0 5 0.001131485911497647 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.6519201050726105E-4 12-13 0.0 14-15 8.839733683575368E-6 16-17 0.0 18-19 3.535893473430147E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.2983307577295958E-4 40-41 7.955760315217832E-5 42-43 0.0013701587209541822 44-45 6.894992273188787E-4 46-47 0.0062231725132370595 48-49 0.004534783379674163 50-51 0.008945810487778273 52-53 0.0038541238860388604 54-55 0.02023415040170402 56-57 0.010678398289759045 58-59 0.020340227205906922 60-61 0.009096085960399054 62-63 0.005825384497476167 64-65 0.012658498634879927 66-67 0.03051476067570217 68-69 0.032158951140847186 70-71 0.036127991564772526 72-73 0.031009785761982392 74-75 0.03371474426915645 76-77 0.03386501974177723 78-79 0.01765294816610001 80-81 0.0022894910240460202 82-83 0.0 84-85 0.006797755202669458 86-87 0.02679323279491694 88-89 0.0275269306906537 90-91 0.02551147141079851 92-93 0.03466059577329902 94-95 0.031213099636704624 96-97 0.03243298288503803 98-99 0.03805505350779196 100-101 0.02704958507174063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5656279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.021717840956974 #Duplication Level Percentage of deduplicated Percentage of total 1 80.82862079576574 23.45785426208421 2 8.402488824759038 4.877093196679019 3 2.690642227768995 2.34261178635827 4 1.7616961103749824 2.0450978974725658 5 1.032994791579084 1.4989641686193167 6 0.6951987376085046 1.21055169637581 7 0.5760084953188561 1.1701729217596621 8 0.3705191035374757 0.8602480702039154 9 0.3574344478446417 0.9336025522786898 >10 2.5763733892342335 14.99674320290806 >50 0.3461770565726184 7.031870414670327 >100 0.3026713145732969 18.50059896837776 >500 0.03736830650020461 7.6470317758534145 >1k 0.021010424169915637 10.464893649861946 >5k 4.898393032703359E-4 0.9266782523062036 >10k+ 3.061350892917934E-4 2.0359871841908452 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 43528 0.7695518555573372 TruSeq Adapter, Index 7 (97% over 38bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 23883 0.42223871912966104 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23529 0.4159801876816897 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12983 0.229532524827718 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10657 0.1884100837317254 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7911 0.13986226634152946 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7867 0.13908436977737484 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7332 0.1296258547359492 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6902 0.12202368376807438 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6103 0.10789778934172094 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5814 0.1027884232726144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.889482820773162E-4 0.0 0.0 2 4.2430721681161767E-4 0.0 0.0 3 4.773456189130699E-4 0.0 0.0 4 5.127045536473713E-4 0.0 0.0 5 5.657429557488236E-4 0.0 0.0 6 6.364608252174266E-4 0.0 0.0 7 7.248581620531802E-4 0.0 0.0 8 9.546912378261398E-4 0.0 0.0 9 0.0010961269767633456 0.0 0.0 10-11 0.0017679467367150738 0.0 0.0 12-13 0.0025104843661354044 0.0 0.0 14-15 0.0036861689460509287 0.0 0.0 16-17 0.00505632766700511 0.0 0.0 18-19 0.006673998931099403 0.0 0.0 20-21 0.00866293900990386 0.0 0.0 22-23 0.010881712164481278 0.0 0.0 24-25 0.013807664013744725 0.0 0.0 26-27 0.016397705983032307 0.0 0.0 28-29 0.019235260495460003 0.0 0.0 30-31 0.030258408398878486 0.0 0.0 32-33 0.03462523683856472 0.0 0.0 34-35 0.03788709856780403 0.0 0.0 36-37 0.04182078005699506 0.0 0.0 38-39 0.04645280050718856 0.0 0.0 40-41 0.05212790953204395 0.0 0.0 42-43 0.058510197251585364 0.0 0.0 44-45 0.06573225967106644 0.0 0.0 46-47 0.0736791802526007 0.0 0.0 48-49 0.08262499074037896 0.0 0.0 50-51 0.09284372287859209 0.0 0.0 52-53 0.10484808122088744 0.0 0.0 54-55 0.11813420094730122 0.0 0.0 56-57 0.13276396019361847 0.0 0.0 58-59 0.14901139070402997 0.0 0.0 60-61 0.1670974858206252 0.0 0.0 62-63 0.18760566796652003 0.0 0.0 64-65 0.21100444302694404 0.0 0.0 66-67 0.2371877341976943 0.0 0.0 68-69 0.2657489137293263 0.0 0.0 70-71 0.2987387998364296 0.0 0.0 72-73 0.3360424759811176 0.0 0.0 74-75 0.37804005071178415 0.0 0.0 76-77 0.42424533867583264 0.0 0.0 78-79 0.47843290615614964 0.0 0.0 80-81 0.538985081888641 0.0 0.0 82-83 0.6062996538890674 0.0 0.0 84-85 0.6828252283877794 0.0 0.0 86-87 0.7653441423239553 0.0 0.0 88-89 0.8563845595310982 0.0 8.839733683575368E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGTT 61975 0.0 46.822285 1 ACGTTTG 140 0.0 44.180008 1 CGGAAGA 11350 0.0 42.514393 4 GTCGCGC 45 9.5503806E-4 42.291977 1 TATGCCG 5615 0.0 42.212654 48-49 GCCGTCT 5730 0.0 41.98755 50-51 TCGTATG 5780 0.0 41.90863 44-45 ATGCCGT 5720 0.0 41.85298 48-49 GAAGAGC 11550 0.0 41.776737 6 CGTATGC 5700 0.0 41.747803 46-47 TCTCGTA 5770 0.0 41.52816 42-43 TGCCGTC 5730 0.0 41.448715 50-51 TCGGAAG 11735 0.0 41.160786 3 CTCGTAT 5760 0.0 41.06464 44-45 CATCTCG 5815 0.0 40.756477 40-41 ATCTCGT 5890 0.0 40.036976 42-43 CGAGTCG 60 8.2145954E-5 39.648727 1 CTGCTTG 6205 0.0 39.310604 58-59 GATCGGA 12320 0.0 39.27541 1 CTTGAAA 5865 0.0 39.04168 62-63 >>END_MODULE