FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34310-1.34100000035813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34310-1.34100000035813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5656279
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT435280.7695518555573372TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA238830.42223871912966104No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC235290.4159801876816897No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT129830.229532524827718No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC106570.1884100837317254No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC79110.13986226634152946No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT78670.13908436977737484No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC73320.1296258547359492No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA69020.12202368376807438No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT61030.10789778934172094No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG58140.1027884232726144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTT619750.046.8222851
ACGTTTG1400.044.1800081
CGGAAGA113500.042.5143934
GTCGCGC459.5503806E-442.2919771
TATGCCG56150.042.21265448-49
GCCGTCT57300.041.9875550-51
TCGTATG57800.041.9086344-45
ATGCCGT57200.041.8529848-49
GAAGAGC115500.041.7767376
CGTATGC57000.041.74780346-47
TCTCGTA57700.041.5281642-43
TGCCGTC57300.041.44871550-51
TCGGAAG117350.041.1607863
CTCGTAT57600.041.0646444-45
CATCTCG58150.040.75647740-41
ATCTCGT58900.040.03697642-43
CGAGTCG608.2145954E-539.6487271
CTGCTTG62050.039.31060458-59
GATCGGA123200.039.275411
CTTGAAA58650.039.0416862-63
Produced by FastQC (version 0.11.2)