##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34302-1.341000000358ea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8204716 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67808526218336 33.0 33.0 33.0 33.0 33.0 2 32.699684059752954 33.0 33.0 33.0 33.0 33.0 3 32.73299843650896 33.0 33.0 33.0 33.0 33.0 4 36.67021491054657 37.0 37.0 37.0 37.0 37.0 5 36.643738308553274 37.0 37.0 37.0 37.0 37.0 6 36.523351082475 37.0 37.0 37.0 37.0 37.0 7 36.429731388630636 37.0 37.0 37.0 37.0 37.0 8 36.36953637395859 37.0 37.0 37.0 37.0 37.0 9 36.43175595596484 37.0 37.0 37.0 37.0 37.0 10-11 36.35291331229503 37.0 37.0 37.0 37.0 37.0 12-13 36.32799099932283 37.0 37.0 37.0 37.0 37.0 14-15 38.16163076211291 40.0 37.0 40.0 37.0 40.0 16-17 38.175097894918 40.0 37.0 40.0 37.0 40.0 18-19 38.14759858842159 40.0 37.0 40.0 37.0 40.0 20-21 38.1296571386505 40.0 37.0 40.0 37.0 40.0 22-23 37.96948322160084 40.0 37.0 40.0 33.0 40.0 24-25 37.99763331235353 40.0 37.0 40.0 35.0 40.0 26-27 37.93223372996701 40.0 37.0 40.0 33.0 40.0 28-29 37.76767123932139 40.0 37.0 40.0 33.0 40.0 30-31 37.75007617570188 40.0 37.0 40.0 33.0 40.0 32-33 37.61683006456287 40.0 37.0 40.0 33.0 40.0 34-35 37.456764195128756 40.0 37.0 40.0 33.0 40.0 36-37 37.18570715915091 40.0 37.0 40.0 33.0 40.0 38-39 37.32192625558277 40.0 37.0 40.0 33.0 40.0 40-41 37.106098248860775 38.5 37.0 40.0 33.0 40.0 42-43 36.9339244649053 37.0 37.0 40.0 33.0 40.0 44-45 36.958949767426446 37.0 37.0 40.0 33.0 40.0 46-47 36.936023257843416 37.0 37.0 40.0 33.0 40.0 48-49 36.62154259818378 37.0 37.0 40.0 33.0 40.0 50-51 36.512019489766615 37.0 37.0 40.0 33.0 40.0 52-53 36.43918430570906 37.0 37.0 40.0 33.0 40.0 54-55 36.18303784067602 37.0 37.0 40.0 33.0 40.0 56-57 36.042392935965125 37.0 37.0 40.0 33.0 40.0 58-59 35.77623966509017 37.0 37.0 40.0 27.0 40.0 60-61 35.50218837556352 37.0 33.0 40.0 27.0 40.0 62-63 35.295619433993814 37.0 33.0 38.5 27.0 40.0 64-65 34.992041406430154 37.0 33.0 37.0 27.0 40.0 66-67 34.434668610101795 37.0 33.0 37.0 27.0 40.0 68-69 34.226295827911656 37.0 33.0 37.0 27.0 40.0 70-71 33.835076497468044 37.0 33.0 37.0 27.0 38.5 72-73 33.1248206519275 37.0 33.0 37.0 24.5 37.0 74-75 32.31457170485852 37.0 33.0 37.0 22.0 37.0 76-77 29.716851320630717 33.0 27.0 35.0 18.5 37.0 78-79 31.904164568280002 33.0 33.0 37.0 22.0 37.0 80-81 32.23173129941365 37.0 33.0 37.0 22.0 37.0 82-83 32.155594111971695 37.0 33.0 37.0 22.0 37.0 84-85 32.024453436292006 37.0 33.0 37.0 15.0 37.0 86-87 31.869314367493036 33.0 33.0 37.0 15.0 37.0 88-89 31.738692174110597 33.0 33.0 37.0 15.0 37.0 90-91 31.587597913200163 33.0 33.0 37.0 15.0 37.0 92-93 31.41040018935451 33.0 33.0 37.0 15.0 37.0 94-95 31.314511251821514 33.0 33.0 37.0 15.0 37.0 96-97 31.186478118194465 33.0 33.0 37.0 15.0 37.0 98-99 30.89560260221073 33.0 33.0 37.0 15.0 37.0 100-101 29.364793979462544 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09250669498811703 2306 2 0.13873887493933523 2306 3 0.13635724809626737 2306 4 0.1908373920222033 2306 5 0.19589692250661983 2306 6 0.22002244031195772 2306 7 0.2522878230464727 2306 8 0.22824504444931648 2306 9 0.20539647856595877 2306 10-11 0.26737004313245905 2306 12-13 0.260988892383736 2306 14-15 0.507163291568169 2306 16-17 0.538479131750357 2306 18-19 0.5646817422030423 2306 20-21 0.5683596536167883 2306 22-23 0.48199166888813494 2306 24-25 0.5965266246722294 2306 26-27 0.6080335063405755 2306 28-29 0.56961984643128 2306 30-31 0.5846092910082277 2306 32-33 0.5967468285837825 2306 34-35 0.6745971729812368 2306 36-37 0.7116739101429275 2306 38-39 0.6741034659986056 2306 40-41 0.6710276410923228 2306 42-43 0.7691557903086945 2306 44-45 0.7303079786272875 2306 46-47 0.7022866842321847 2306 48-49 0.7876666914518395 2306 50-51 0.6806562563988194 2306 52-53 0.6426641572834271 2306 54-55 0.6684029886186238 2306 56-57 0.772953561727789 2306 58-59 0.7431103338885592 2306 60-61 0.6665691835928484 2306 62-63 0.7470855183270686 2306 64-65 0.686885299140819 2306 66-67 0.6436981194779037 2306 68-69 0.6607910052865975 2306 70-71 0.6514950324980404 2306 72-73 0.37991263570436473 2306 74-75 0.45707467199662233 2306 76-77 0.24505677983772856 2306 78-79 0.6235093226400998 2306 80-81 0.7535111252516273 2306 82-83 0.7559033465916869 2306 84-85 0.6851235002214651 2306 86-87 0.6555875193561462 2306 88-89 0.6851341646621734 2306 90-91 0.8007547306684302 2306 92-93 0.6989177757671854 2306 94-95 0.7005315405936443 2306 96-97 0.7323530794933468 2306 98-99 0.7049750003908528 2306 100-101 0.6245468628139825 2307 1 0.10330514352787645 2307 2 0.07381765261548168 2307 3 0.11482748802561105 2307 4 0.1618153126994457 2307 5 0.17711633158599938 2307 6 0.1971656485915645 2307 7 0.20374305013269378 2307 8 0.2504838691803144 2307 9 0.21111208377971735 2307 10-11 0.24710733937656926 2307 12-13 0.26335383625522724 2307 14-15 0.4702973815338254 2307 16-17 0.4774520769799082 2307 18-19 0.4649958096020512 2307 20-21 0.531085395546306 2307 22-23 0.5619280626139727 2307 24-25 0.53396174042242 2307 26-27 0.5363239897285155 2307 28-29 0.5951537779559573 2307 30-31 0.583888408112351 2307 32-33 0.5757371003486753 2307 34-35 0.6159514926715843 2307 36-37 0.6659553606854658 2307 38-39 0.6200574812735979 2307 40-41 0.6706843522954671 2307 42-43 0.6843763956528619 2307 44-45 0.6077519624749215 2307 46-47 0.6028134946068278 2307 48-49 0.6176322844715969 2307 50-51 0.6276634208725511 2307 52-53 0.6145517873779553 2307 54-55 0.5450732759093242 2307 56-57 0.5796763104902283 2307 58-59 0.6043327432082819 2307 60-61 0.6310038099619888 2307 62-63 0.5462985446750963 2307 64-65 0.516046235363163 2307 66-67 0.5712509209817966 2307 68-69 0.5389473841202843 2307 70-71 0.44108674782651036 2307 72-73 0.28930889922626335 2307 74-75 0.33911111607521605 2307 76-77 0.07851635609356578 2307 78-79 0.45704313863489077 2307 80-81 0.5307387263428538 2307 82-83 0.5981981591083567 2307 84-85 0.5435987886888256 2307 86-87 0.5650048552930258 2307 88-89 0.5865771388021841 2307 90-91 0.5855832255247719 2307 92-93 0.61430575208297 2307 94-95 0.6352544154958579 2307 96-97 0.5912220242501682 2307 98-99 0.6070762001480645 2307 100-101 0.4910939914174577 2201 1 0.10385370910196912 2201 2 0.09983264035093242 2201 3 0.10348674724994567 2201 4 0.1299068199112554 2201 5 0.20064580343252914 2201 6 0.15723711453917133 2201 7 0.22962973543581455 2201 8 0.2104946802119656 2201 9 0.20557347284014327 2201 10-11 0.22855227775526288 2201 12-13 0.2240604110281197 2201 14-15 0.43361612468854105 2201 16-17 0.4334898731636798 2201 18-19 0.44778910204831845 2201 20-21 0.3950357905103843 2201 22-23 0.4242792341495374 2201 24-25 0.4087615870566168 2201 26-27 0.4593213561109266 2201 28-29 0.36902136953152365 2201 30-31 0.49323661132832086 2201 32-33 0.4948535735010182 2201 34-35 0.5175942037891872 2201 36-37 0.5219137633555349 2201 38-39 0.5794120878258937 2201 40-41 0.5972430343552801 2201 42-43 0.5867166383039546 2201 44-45 0.6344352935297692 2201 46-47 0.675291915756226 2201 48-49 0.7101901148483947 2201 50-51 0.6867514443551173 2201 52-53 0.6787642870047605 2201 54-55 0.6179872810665543 2201 56-57 0.6458501559647587 2201 58-59 0.6505647529759173 2201 60-61 0.6832738909033012 2201 62-63 0.6590228382260364 2201 64-65 0.5292148733145936 2201 66-67 0.5839423335970721 2201 68-69 0.6951751590434085 2201 70-71 0.6488137284216577 2201 72-73 0.5011397505792914 2201 74-75 0.4217250365355625 2201 76-77 0.320702046561788 2201 78-79 0.6525197118521326 2201 80-81 0.6318459928332132 2201 82-83 0.6320646585811502 2201 84-85 0.5186572436855528 2201 86-87 0.6866201617024927 2201 88-89 0.6807411852079142 2201 90-91 0.648008015786047 2201 92-93 0.7177259586553788 2201 94-95 0.7373957225935825 2201 96-97 0.7615950441264374 2201 98-99 0.6782452765119729 2201 100-101 0.4718989497211332 1101 1 0.07171278089703748 1101 2 0.10303515008828867 1101 3 0.11028370043575819 1101 4 0.13007276530642287 1101 5 0.18896428772605844 1101 6 0.11518796973947332 1101 7 0.0785814144060808 1101 8 0.21017290689016477 1101 9 0.14255346332404883 1101 10-11 0.1760856389258123 1101 12-13 0.21042721672960596 1101 14-15 0.43002976575933616 1101 16-17 0.4044723115076394 1101 18-19 0.3792657569825053 1101 20-21 0.4058158114034214 1101 22-23 0.4261517695531154 1101 24-25 0.4145414037091797 1101 26-27 0.41283655235955763 1101 28-29 0.41942985995983406 1101 30-31 0.4241013012496566 1101 32-33 0.45417331273887385 1101 34-35 0.4221383938659784 1101 36-37 0.5002471572045621 1101 38-39 0.5334020959274355 1101 40-41 0.571555349073229 1101 42-43 0.6313690455806622 1101 44-45 0.6133881695762184 1101 46-47 0.5426920894362794 1101 48-49 0.5719845490682118 1101 50-51 0.5269487709227931 1101 52-53 0.6046198844422221 1101 54-55 0.5333444447259765 1101 56-57 0.6087908615475541 1101 58-59 0.5898792311301335 1101 60-61 0.4637979625357289 1101 62-63 0.5857942556776976 1101 64-65 0.5067381605122776 1101 66-67 0.5136861120158116 1101 68-69 0.579795169618528 1101 70-71 0.5582907990230481 1101 72-73 0.49490814640642355 1101 74-75 0.3407242052011412 1101 76-77 0.26540809533290144 1101 78-79 0.6335750356829841 1101 80-81 0.5312887094283596 1101 82-83 0.5070089652263121 1101 84-85 0.4745464046429362 1101 86-87 0.5053907228570154 1101 88-89 0.5962465177757252 1101 90-91 0.5770930713583624 1101 92-93 0.6556186488127338 1101 94-95 0.6069307294129445 1101 96-97 0.6505901979535658 1101 98-99 0.6003584507041495 1101 100-101 0.557307309662459 2304 1 0.06267738978154824 2304 2 0.07912967603810728 2304 3 0.09000658718149879 2304 4 0.10987556215139449 2304 5 0.15800273915594687 2304 6 0.14709421388656096 2304 7 0.13026861897474618 2304 8 0.16864230144974357 2304 9 0.11851993748634015 2304 10-11 0.14130516162224183 2304 12-13 0.16079133928099765 2304 14-15 0.3260347562492214 2304 16-17 0.3152945545326631 2304 18-19 0.2988266945784588 2304 20-21 0.31533944089206045 2304 22-23 0.35541292775027244 2304 24-25 0.2841852793974766 2304 26-27 0.3379079802505274 2304 28-29 0.27953567428499326 2304 30-31 0.31807909925564104 2304 32-33 0.3466061112798542 2304 34-35 0.342693880478933 2304 36-37 0.3986279421386385 2304 38-39 0.4118282786436893 2304 40-41 0.40051424593791296 2304 42-43 0.39479754649781995 2304 44-45 0.41512125121754195 2304 46-47 0.370746479677571 2304 48-49 0.4859588818933034 2304 50-51 0.42938653503227187 2304 52-53 0.41387975106422914 2304 54-55 0.39413828755714064 2304 56-57 0.4818174669750519 2304 58-59 0.4537234368016314 2304 60-61 0.4865390307640709 2304 62-63 0.3991566808518243 2304 64-65 0.3631166939293209 2304 66-67 0.37743608628638015 2304 68-69 0.45270870374408645 2304 70-71 0.4558456796971555 2304 72-73 0.3042363555135097 2304 74-75 0.22879186672798824 2304 76-77 0.06755003046886543 2304 78-79 0.4011888439950262 2304 80-81 0.2577887504253411 2304 82-83 0.3447145731886394 2304 84-85 0.23747833807948382 2304 86-87 0.2906711698806532 2304 88-89 0.3994907359674613 2304 90-91 0.46403693066487506 2304 92-93 0.41160154005623184 2304 94-95 0.4029323346745777 2304 96-97 0.30380825505028497 2304 98-99 0.3614504317788487 2304 100-101 0.38653343117242045 2202 1 0.06859020319463127 2202 2 0.07864392506681384 2202 3 0.07900370592047068 2202 4 0.10999655300332023 2202 5 0.14703877626273254 2202 6 0.11822205050464873 2202 7 0.1486353100902278 2202 8 0.09660475235562416 2202 9 0.1556772443049752 2202 10-11 0.14586131143258285 2202 12-13 0.14298398069393414 2202 14-15 0.19068245340781687 2202 16-17 0.20154428715104444 2202 18-19 0.19749234831834173 2202 20-21 0.2208701548191101 2202 22-23 0.19506440453717744 2202 24-25 0.2238553390735376 2202 26-27 0.2587275311286845 2202 28-29 0.15558428676551728 2202 30-31 0.24276722915928417 2202 32-33 0.18912533550602006 2202 34-35 0.21776206291401223 2202 36-37 0.24119633407811847 2202 38-39 0.2764703022309405 2202 40-41 0.17311660294540587 2202 42-43 0.2730654094408962 2202 44-45 0.26857734314774007 2202 46-47 0.2691029334941817 2202 48-49 0.3050449060781446 2202 50-51 0.2642415901483943 2202 52-53 0.29954470751830087 2202 54-55 0.33696869162753273 2202 56-57 0.33932240983120465 2202 58-59 0.4039398204008009 2202 60-61 0.34707767302132453 2202 62-63 0.40455760017634645 2202 64-65 0.2933722197303652 2202 66-67 0.43027250181649634 2202 68-69 0.3664206589751089 2202 70-71 0.2535704843237454 2202 72-73 0.20071494465020123 2202 74-75 0.1244625257041676 2202 76-77 0.09752590749273082 2202 78-79 0.2835621284221794 2202 80-81 0.3055772710068325 2202 82-83 0.4071942141510547 2202 84-85 0.3399929329045932 2202 86-87 0.3288472033513088 2202 88-89 0.38330364832025055 2202 90-91 0.31477450756921144 2202 92-93 0.28326498706831416 2202 94-95 0.36774432058368234 2202 96-97 0.3515956001144289 2202 98-99 0.42785378450200184 2202 100-101 0.319045571400725 1102 1 0.053949277667129536 1102 2 0.05697936833394124 1102 3 0.09308122037433009 1102 4 0.0799160799102836 1102 5 0.1070802933372974 1102 6 0.09884707898223866 1102 7 0.11208876849156724 1102 8 0.1374716472471036 1102 9 0.050434161163479985 1102 10-11 0.06730845220778292 1102 12-13 0.07679096851182265 1102 14-15 0.13986760744894156 1102 16-17 0.15974173218788934 1102 18-19 0.1609106367257951 1102 20-21 0.1585133037388644 1102 22-23 0.22440322407055646 1102 24-25 0.14089809605653159 1102 26-27 0.15647030869264 1102 28-29 0.1540355576091912 1102 30-31 0.1768736005532574 1102 32-33 0.17295508679009686 1102 34-35 0.15940180446480667 1102 36-37 0.20900361442102877 1102 38-39 0.1084934934790951 1102 40-41 0.19444003997944748 1102 42-43 0.11911209743498574 1102 44-45 0.12307838726856346 1102 46-47 0.14129174643750986 1102 48-49 0.1491315381468965 1102 50-51 0.121009543739099 1102 52-53 0.16062666094111222 1102 54-55 0.07698970430324437 1102 56-57 0.159211155683046 1102 58-59 0.08783308502531639 1102 60-61 -0.05547148428913573 1102 62-63 0.16476156381072826 1102 64-65 0.17392367566443312 1102 66-67 0.23189861027607606 1102 68-69 0.25559998165578435 1102 70-71 0.08963421075765865 1102 72-73 0.06803285964961248 1102 74-75 0.0863833645608949 1102 76-77 -0.03776519026671821 1102 78-79 0.23367706757755613 1102 80-81 0.1480326055133574 1102 82-83 0.3328913150642663 1102 84-85 0.29414774265778476 1102 86-87 0.2689453401285036 1102 88-89 0.27987310916451236 1102 90-91 0.3631699875843424 1102 92-93 0.38205401745417333 1102 94-95 0.36213902638043294 1102 96-97 0.3816155957047549 1102 98-99 0.2690001997651521 1102 100-101 0.19334086228474945 2305 1 -0.0014425187722366672 2305 2 -0.02569544293943693 2305 3 0.018764286346012682 2305 4 0.018842848438488602 2305 5 0.05529580056835215 2305 6 0.018499788538136386 2305 7 0.003887554648741798 2305 8 0.035913924641135964 2305 9 -0.01045734574265822 2305 10-11 -0.026409020004948047 2305 12-13 -0.03848469981566893 2305 14-15 -0.0636118855652299 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1213 60-61 -0.531586503697838 1213 62-63 -0.2760360014039591 1213 64-65 -0.6110443081059955 1213 66-67 -0.5583638298915119 1213 68-69 -0.07250254032066294 1213 70-71 0.07705891168725287 1213 72-73 0.26096281552216993 1213 74-75 0.3128413755239734 1213 76-77 -2.111773302204945 1213 78-79 -1.8903027923322178 1213 80-81 -1.3056507191796634 1213 82-83 -0.899460108431942 1213 84-85 -0.5814850934770561 1213 86-87 -0.33053846253433505 1213 88-89 -0.39194255124731114 1213 90-91 -1.577021622783473 1213 92-93 -0.7878859884359031 1213 94-95 -0.4462429066799345 1213 96-97 -0.32896424448386696 1213 98-99 -2.1065464958638103 1213 100-101 -0.7449468688929564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 19.0 8 152.0 9 418.0 10 335.0 11 174.0 12 156.0 13 238.0 14 612.0 15 1369.0 16 2763.0 17 4759.0 18 7702.0 19 11007.0 20 15729.0 21 21461.0 22 28868.0 23 37834.0 24 49829.0 25 65806.0 26 88881.0 27 130667.0 28 211255.0 29 292102.0 30 225533.0 31 190734.0 32 246481.0 33 352994.0 34 542131.0 35 929465.0 36 1742251.0 37 2439130.0 38 563716.0 39 144.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.073743521497335 24.252595979512975 23.36492665327725 14.308733845712437 2 27.41956106810913 27.14268876103208 32.48835375005043 12.949396420808364 3 34.79913292067638 18.26061254997986 31.091728923750285 15.848525605593478 4 26.394624749960876 18.238985968557593 33.44731249686156 21.919076784619968 5 35.81951206551036 17.317096477036248 31.373118043406244 15.490273414047149 6 29.26693623520912 18.418224348045683 36.82048226897799 15.494357147767209 7 30.211198047561915 24.868112436798544 28.44230074508368 16.47838877055586 8 26.315852980164088 31.630357467583277 25.814653426151494 16.239136126101137 9 34.54037897228862 23.30068463064413 24.500494593597146 17.6584418034701 10-11 26.22674228838161 29.44155287616924 27.404591857006803 16.927112978442352 12-13 23.067714958080206 27.835405881202956 27.084380495315134 22.012498665401704 14-15 22.25759077203835 28.471230964681226 27.541917815431873 21.72926044784856 16-17 24.52206755236866 26.212772020384374 28.36571064738865 20.899449779858315 18-19 20.340007059358086 26.432018576642303 31.807770949181396 21.420203414818218 20-21 23.67415276774967 26.712137263495773 31.390800120321042 18.22290984843351 22-23 20.616904960512954 33.15476123731766 28.001993000123342 18.226340802046042 24-25 20.723124359209997 26.066971726992133 31.580148538962227 21.629755374835643 26-27 20.490087652028418 26.155292882776198 28.216479400383875 25.138140064811505 28-29 23.16853502302822 29.76315085129089 27.91369622056388 19.154617905117007 30-31 19.381133972217928 27.066427405896803 31.159865862511264 22.392572759373998 32-33 19.36365012512316 30.14040339726567 28.41334179025819 22.08260468735298 34-35 22.888866598185725 30.35996614629927 28.482204624754836 18.268962630760164 36-37 19.373428533070832 30.853316102589556 31.789003749003086 17.984251615336525 38-39 19.914389240401803 27.273756617097067 30.839014939376767 21.97283920312436 40-41 23.195603500915936 26.851875325270484 30.69154790358221 19.260973270231364 42-43 20.065323051712102 30.232378575087232 31.146249226425514 18.55604914677515 44-45 19.496269469641327 27.384727309690724 31.32134775285449 21.797655467813463 46-47 23.046540117130835 27.17808446591274 28.112082715233562 21.66329270172287 48-49 20.23022842633871 30.544239605863872 30.636653731086234 18.58887823671119 50-51 23.237758342788524 27.438179439865433 30.07819982492786 19.245862392418182 52-53 19.592861423671085 27.28959076863934 27.332714070689796 25.78483373699978 54-55 23.06737039322309 27.22337669889456 30.446862624901843 19.26239028298051 56-57 19.877475124800345 26.955704143815733 30.11996813429283 23.046852597091096 58-59 19.76792629259473 26.74888046671718 30.740251516396246 22.742941724291846 60-61 22.653219734437776 26.990360622495036 30.938640189674416 19.41777945339277 62-63 19.616829297233735 27.09538745782129 30.7568956666675 22.53088757827747 64-65 23.113513093135673 27.163642742142315 30.48259514501283 19.240249019709186 66-67 20.18341370232846 33.411739432793865 26.89617549896564 19.508671365912036 68-69 20.13913793659447 33.27991393398976 26.966195694468347 19.61475243494742 70-71 19.74119212297029 34.32487523045468 26.92973828297744 19.00419436359759 72-73 19.716294071497064 34.43310934748246 26.744166624502952 19.106429956517527 74-75 20.513175182897804 33.30396621326248 26.678356211014826 19.504502392824897 76-77 20.79303787066339 32.0547357471108 27.26832364535311 19.883902736872702 78-79 20.64051479917811 31.67468510905368 27.814660398195766 19.87013969357244 80-81 20.10150514777848 31.932815000530194 27.608699361692533 20.356980489998794 82-83 19.33272279016117 31.657805096483532 28.04485859108347 20.964613522271826 84-85 19.480676036293342 31.144128598939723 28.28129771753008 21.093897647236854 86-87 19.38768276305747 31.105237495282744 28.813005446020767 20.69407429563902 88-89 19.597402126369744 31.39968381504247 28.492232986923767 20.51068107166402 90-91 20.10252422800373 30.661872360341263 28.085947626781188 21.149655784873822 92-93 19.698680697029726 31.40238441879624 27.77526749819644 21.123667385977598 94-95 19.4750120426021 31.942156251832603 27.573023400869843 21.00980830469545 96-97 19.937816245988852 32.232668410676126 27.05090227282273 20.77861307051229 98-99 20.226207188509708 31.14798962404698 27.018970455699765 21.60683273174355 100-101 20.272543564172178 31.351976542183614 26.726460455166634 21.649019438477577 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104.0 1 74.5 2 133.0 3 361.0 4 398.5 5 244.0 6 189.5 7 177.5 8 197.5 9 215.0 10 200.0 11 192.0 12 197.0 13 203.5 14 215.5 15 254.5 16 322.0 17 408.0 18 543.5 19 757.0 20 1121.0 21 1720.5 22 2573.0 23 3866.0 24 5585.0 25 7979.5 26 11458.5 27 16078.5 28 21447.0 29 27131.5 30 33844.0 31 41789.5 32 53042.0 33 77940.5 34 120638.0 35 200611.0 36 312377.5 37 432944.0 38 567842.5 39 636737.0 40 640936.0 41 598548.5 42 537253.5 43 529086.5 44 505639.0 45 463681.0 46 450982.5 47 399565.0 48 305295.0 49 246194.5 50 203538.5 51 167211.5 52 144519.5 53 120116.0 54 89188.0 55 59656.5 56 41286.0 57 30154.5 58 23101.0 59 17719.5 60 13155.0 61 9952.5 62 7186.0 63 5067.5 64 3827.5 65 2862.5 66 2064.0 67 1488.0 68 1101.0 69 741.0 70 458.5 71 303.0 72 205.0 73 135.5 74 113.5 75 96.0 76 64.5 77 42.0 78 30.5 79 23.5 80 12.5 81 11.0 82 14.5 83 12.5 84 7.0 85 3.0 86 1.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17265679884593202 2 0.006983788348067135 3 0.0019622860803469614 4 0.0 5 0.0010969301070262517 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.1938602140525036E-4 12-13 0.0 14-15 6.094056150145843E-6 16-17 0.0 18-19 4.8752449201166745E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.8282168450437527E-5 38-39 2.803265829067088E-4 40-41 7.312867380175011E-5 42-43 0.001322410184581648 44-45 6.276877834650218E-4 46-47 0.005746694949587529 48-49 0.0041744284628499025 50-51 0.008159941185045283 52-53 0.004083017620597715 54-55 0.019799588431823844 56-57 0.011121652474016162 58-59 0.019897093330226177 60-61 0.009299529685122557 62-63 0.005941704746392197 64-65 0.012529379444699854 66-67 0.031055310141143218 68-69 0.032170522416619905 70-71 0.0365033963393736 72-73 0.031213755601047007 74-75 0.034120620384666574 76-77 0.03412671444081672 78-79 0.01754478765626988 80-81 0.0025107511338600874 82-83 0.0 84-85 0.006234219441599198 86-87 0.02585708024506881 88-89 0.027283089384202937 90-91 0.025223298405453645 92-93 0.03516270398634151 94-95 0.03150627029625401 96-97 0.033328393085147616 98-99 0.03964183525669871 100-101 0.027392782394905568 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8204716.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.37773099102915 #Duplication Level Percentage of deduplicated Percentage of total 1 89.2044356080102 30.66646090538757 2 4.805916085620572 3.304329807138477 3 1.7154733175828996 1.7692224070245972 4 0.8442078550980379 1.1608780217229624 5 0.529370733346605 0.9099282332756706 6 0.38976497170210433 0.803954120814062 7 0.30610142066780155 0.7366150606982673 8 0.23274347399618112 0.6400954031166644 9 0.1662381270853623 0.5143400652053792 >10 1.3906230232153012 9.84592903039009 >50 0.1764156511310822 4.252244660510393 >100 0.17982140388178436 13.677922259015816 >500 0.03127555264341525 7.378812908064068 >1k 0.026832509697929226 16.493816519123996 >5k 5.321297144226714E-4 1.1330742030892944 >10k+ 2.481366062788748E-4 6.71237639542272 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 476049 5.802138672441557 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16097 0.19619204369779528 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 13542 0.16505141677055002 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 12998 0.15842108367919133 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11303 0.13776223333019694 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 10602 0.12921836660769245 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10225 0.12462344827048248 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9798 0.11941912431825794 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8743 0.10656066584145021 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002047602866449003 0.0 0.0 2 0.0023035532247551284 0.0 0.0 3 0.0025351273584606707 0.0 0.0 4 0.002754513379865921 0.0 0.0 5 0.0030470280750729216 0.0 0.0 6 0.003485800117883422 0.0 0.0 7 0.003961136497594798 0.0 0.0 8 0.004619294561810549 0.0 0.0 9 0.005338393187527758 0.0 0.0 10-11 0.008696218126258117 0.0 0.0 12-13 0.011651835359078853 0.0 0.0 14-15 0.01511325925236169 0.0 0.0 16-17 0.017892148856828195 0.0 0.0 18-19 0.021621711220717453 0.0 1.2188112300291686E-5 20-21 0.025631600167513417 0.0 1.2188112300291686E-5 22-23 0.03074451327748578 0.0 1.828216845043753E-5 24-25 0.03631448059871908 0.0 2.4376224600583373E-5 26-27 0.042109927997507776 0.0 2.4376224600583373E-5 28-29 0.04820398414765362 0.0 2.4376224600583373E-5 30-31 0.073750267529065 0.0 2.4376224600583373E-5 32-33 0.08133736743599657 0.0 2.4376224600583373E-5 34-35 0.08786410157280276 0.0 2.4376224600583373E-5 36-37 0.09437255354115853 1.2188112300291686E-5 2.4376224600583373E-5 38-39 0.10197793561654053 1.2188112300291686E-5 2.4376224600583373E-5 40-41 0.10997333728553188 1.2188112300291686E-5 2.4376224600583373E-5 42-43 0.1181515606390276 1.2188112300291686E-5 2.4376224600583373E-5 44-45 0.12713419940434256 1.2188112300291686E-5 3.0470280750729215E-5 46-47 0.13700657036757885 1.2188112300291686E-5 3.656433690087506E-5 48-49 0.14758585184423204 1.2188112300291686E-5 3.656433690087506E-5 50-51 0.15878063299204992 1.2188112300291686E-5 3.656433690087506E-5 52-53 0.17011557743132122 1.2188112300291686E-5 3.656433690087506E-5 54-55 0.18237681840541464 1.2188112300291686E-5 3.656433690087506E-5 56-57 0.19544856884747747 1.2188112300291686E-5 3.656433690087506E-5 58-59 0.20877626964784643 1.2188112300291686E-5 3.656433690087506E-5 60-61 0.22416376142696467 1.2188112300291686E-5 3.656433690087506E-5 62-63 0.2411966483666223 1.2188112300291686E-5 3.656433690087506E-5 64-65 0.25900348043734844 1.2188112300291686E-5 3.656433690087506E-5 66-67 0.2787238461392204 1.2188112300291686E-5 3.656433690087506E-5 68-69 0.3011743489963577 1.2188112300291686E-5 3.656433690087506E-5 70-71 0.3258735585728988 1.2188112300291686E-5 3.656433690087506E-5 72-73 0.3526447472404895 1.828216845043753E-5 4.8752449201166745E-5 74-75 0.3819388751542405 2.4376224600583373E-5 4.8752449201166745E-5 76-77 0.41463348639977304 2.4376224600583373E-5 4.8752449201166745E-5 78-79 0.451947392207116 2.4376224600583373E-5 5.484650535131259E-5 80-81 0.4948068891110917 2.4376224600583373E-5 7.312867380175012E-5 82-83 0.5415300176142599 2.4376224600583373E-5 7.922272995189596E-5 84-85 0.593305118665899 2.4376224600583373E-5 8.53167861020418E-5 86-87 0.6507415978810236 2.4376224600583373E-5 9.750489840233349E-5 88-89 0.7170814931315113 2.4376224600583373E-5 1.0969301070262517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 62675 0.0 82.57069 4 TCGGAAG 62895 0.0 82.554695 3 GATCGGA 63260 0.0 82.3586 1 ATCGGAA 63800 0.0 81.45066 2 GAAGAGC 63730 0.0 81.126305 6 GGAAGAG 66820 0.0 77.469955 5 AGAGCAC 91270 0.0 56.595055 8 GAGCACA 91310 0.0 56.56506 9 AAGAGCA 92295 0.0 55.90478 7 TTGTGTT 94570 0.0 47.03082 1 GCCGTCT 55865 0.0 45.844128 50-51 ATGCCGT 55820 0.0 45.722115 48-49 TCGTATG 56250 0.0 45.68087 44-45 TGCCGTC 55890 0.0 45.543144 50-51 CGTCTTC 56265 0.0 45.53226 52-53 TCTCGTA 55925 0.0 45.52469 42-43 TATGCCG 55815 0.0 45.504944 48-49 CGTATCT 55540 0.0 45.443016 38-39 CGTATGC 56065 0.0 45.39739 46-47 CTCGTAT 55785 0.0 45.39328 44-45 >>END_MODULE