FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34302-1.341000000358ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34302-1.341000000358ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8204716
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4760495.802138672441557TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC160970.19619204369779528No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA135420.16505141677055002No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG129980.15842108367919133No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA113030.13776223333019694No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC106020.12921836660769245No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC102250.12462344827048248No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA97980.11941912431825794No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG87430.10656066584145021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA626750.082.570694
TCGGAAG628950.082.5546953
GATCGGA632600.082.35861
ATCGGAA638000.081.450662
GAAGAGC637300.081.1263056
GGAAGAG668200.077.4699555
AGAGCAC912700.056.5950558
GAGCACA913100.056.565069
AAGAGCA922950.055.904787
TTGTGTT945700.047.030821
GCCGTCT558650.045.84412850-51
ATGCCGT558200.045.72211548-49
TCGTATG562500.045.6808744-45
TGCCGTC558900.045.54314450-51
CGTCTTC562650.045.5322652-53
TCTCGTA559250.045.5246942-43
TATGCCG558150.045.50494448-49
CGTATCT555400.045.44301638-39
CGTATGC560650.045.3973946-47
CTCGTAT557850.045.3932844-45
Produced by FastQC (version 0.11.2)