##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34298-1.341000000357c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5257116 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.675184835183394 33.0 33.0 33.0 33.0 33.0 2 32.700423958687615 33.0 33.0 33.0 33.0 33.0 3 32.72695827902599 33.0 33.0 33.0 33.0 33.0 4 36.670154320353596 37.0 37.0 37.0 37.0 37.0 5 36.63430481655722 37.0 37.0 37.0 37.0 37.0 6 36.51010934512383 37.0 37.0 37.0 37.0 37.0 7 36.44707040894666 37.0 37.0 37.0 37.0 37.0 8 36.37135608192781 37.0 37.0 37.0 37.0 37.0 9 36.39341532505655 37.0 37.0 37.0 37.0 37.0 10-11 36.33152797084942 37.0 37.0 37.0 37.0 37.0 12-13 36.31098752624062 37.0 37.0 37.0 37.0 37.0 14-15 38.11052019776623 40.0 37.0 40.0 37.0 40.0 16-17 38.087803845302254 40.0 37.0 40.0 37.0 40.0 18-19 38.08371338962275 40.0 37.0 40.0 37.0 40.0 20-21 38.0162165909978 40.0 37.0 40.0 33.0 40.0 22-23 37.9101884949847 40.0 37.0 40.0 33.0 40.0 24-25 37.8718336251283 40.0 37.0 40.0 33.0 40.0 26-27 37.75461631434422 40.0 37.0 40.0 33.0 40.0 28-29 37.63152582138191 40.0 37.0 40.0 33.0 40.0 30-31 37.56078960403384 40.0 37.0 40.0 33.0 40.0 32-33 37.42849054500604 40.0 37.0 40.0 33.0 40.0 34-35 37.34049172588165 40.0 37.0 40.0 33.0 40.0 36-37 37.26415557503391 40.0 37.0 40.0 33.0 40.0 38-39 37.14022593376292 40.0 37.0 40.0 33.0 40.0 40-41 37.033655715415065 40.0 37.0 40.0 33.0 40.0 42-43 36.88133018179549 37.0 37.0 40.0 33.0 40.0 44-45 36.786356816170695 37.0 37.0 40.0 33.0 40.0 46-47 36.691910735848325 37.0 37.0 40.0 33.0 40.0 48-49 36.43266954733356 37.0 37.0 40.0 33.0 40.0 50-51 36.25531622281114 37.0 37.0 40.0 33.0 40.0 52-53 36.14008431619162 37.0 37.0 40.0 33.0 40.0 54-55 35.947745493917196 37.0 37.0 40.0 30.0 40.0 56-57 35.75172689740915 37.0 37.0 40.0 27.0 40.0 58-59 35.45590747854907 37.0 33.0 40.0 27.0 40.0 60-61 35.18965227322357 37.0 33.0 40.0 27.0 40.0 62-63 34.946800774417 37.0 33.0 38.5 27.0 40.0 64-65 34.62144966936245 37.0 33.0 37.0 27.0 40.0 66-67 34.211870729122204 37.0 33.0 37.0 27.0 40.0 68-69 33.92238615240752 37.0 33.0 37.0 27.0 40.0 70-71 33.58166778134627 37.0 33.0 37.0 27.0 38.5 72-73 33.10466727384368 37.0 33.0 37.0 27.0 37.0 74-75 32.56977494885028 37.0 33.0 37.0 22.0 37.0 76-77 29.946782989000052 33.0 30.0 33.0 22.0 37.0 78-79 32.32068647524612 33.0 33.0 37.0 22.0 37.0 80-81 32.74365507628137 37.0 33.0 37.0 27.0 37.0 82-83 32.6987092352537 37.0 33.0 37.0 27.0 37.0 84-85 32.575783661612185 37.0 33.0 37.0 22.0 37.0 86-87 32.42477472439261 33.0 33.0 37.0 22.0 37.0 88-89 32.292354496267535 33.0 33.0 37.0 22.0 37.0 90-91 32.12826928300612 33.0 33.0 37.0 22.0 37.0 92-93 31.93142085888917 33.0 33.0 37.0 22.0 37.0 94-95 31.838307828855214 33.0 33.0 37.0 22.0 37.0 96-97 31.698445497493303 33.0 33.0 37.0 18.5 37.0 98-99 31.396612420193886 33.0 33.0 37.0 15.0 37.0 100-101 29.793744897392408 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16834978094691166 2306 2 0.16143658633892954 2306 3 0.15355572458813782 2306 4 0.19930026228860953 2306 5 0.2101651791274861 2306 6 0.21745696938629777 2306 7 0.24158280819739275 2306 8 0.2542802794783796 2306 9 0.2200255844209167 2306 10-11 0.22376060848110768 2306 12-13 0.22385874613729584 2306 14-15 0.5913119667237652 2306 16-17 0.5999549863240077 2306 18-19 0.6223044808627662 2306 20-21 0.6430179938776988 2306 22-23 0.6280253372694062 2306 24-25 0.6721729146187201 2306 26-27 0.5696537152897321 2306 28-29 0.6703788277224234 2306 30-31 0.6708019421062943 2306 32-33 0.7209763199074857 2306 34-35 0.754368887331772 2306 36-37 0.8163920648805245 2306 38-39 0.8614766644540595 2306 40-41 0.831046628494633 2306 42-43 0.8694621786672414 2306 44-45 0.877320669967375 2306 46-47 0.9019452520244542 2306 48-49 0.9895369653123893 2306 50-51 0.8674064885150798 2306 52-53 0.8849637520054827 2306 54-55 0.9221709969789771 2306 56-57 0.9912079203975637 2306 58-59 1.0345798042923562 2306 60-61 1.0187023005377114 2306 62-63 1.110864227097693 2306 64-65 0.8500063908197859 2306 66-67 0.8339856450605154 2306 68-69 0.9742171733882685 2306 70-71 0.9571224462618062 2306 72-73 0.7440195288856373 2306 74-75 0.79093822603717 2306 76-77 0.40295193998681356 2306 78-79 0.8304175874704782 2306 80-81 0.9286349252270654 2306 82-83 0.9399974968045086 2306 84-85 0.8792531371222196 2306 86-87 0.8365912002016245 2306 88-89 0.8325006290536621 2306 90-91 1.0018571002253083 2306 92-93 1.0104295307123685 2306 94-95 1.0327645501214775 2306 96-97 1.001211746526561 2306 98-99 0.9621679743499492 2306 100-101 0.8402243562230254 2307 1 0.15875135356529313 2307 2 0.07044136282239322 2307 3 0.13789851526466634 2307 4 0.17847686363329984 2307 5 0.21447984702314926 2307 6 0.2033657945155838 2307 7 0.17872541452391744 2307 8 0.26064035453516254 2307 9 0.16784700946524111 2307 10-11 0.2445501469320348 2307 12-13 0.23379217007802566 2307 14-15 0.49062582730437043 2307 16-17 0.40282902793130404 2307 18-19 0.44106068268838783 2307 20-21 0.4542809011211162 2307 22-23 0.48500829900267206 2307 24-25 0.4776469809941233 2307 26-27 0.44780761305385397 2307 28-29 0.48333192350133913 2307 30-31 0.5922528518641457 2307 32-33 0.5373524315332858 2307 34-35 0.5334561353593372 2307 36-37 0.5325239441061953 2307 38-39 0.5804805474043491 2307 40-41 0.6294804372816714 2307 42-43 0.5639045591023049 2307 44-45 0.529460368392094 2307 46-47 0.6741585650416724 2307 48-49 0.6601709192593788 2307 50-51 0.5292866390675854 2307 52-53 0.47304292217965127 2307 54-55 0.4428023643825725 2307 56-57 0.5057477429763679 2307 58-59 0.4782754574886141 2307 60-61 0.5304528887803812 2307 62-63 0.48939826836011235 2307 64-65 0.4272556254380788 2307 66-67 0.5273938784200283 2307 68-69 0.5559268628933296 2307 70-71 0.44939338433055553 2307 72-73 0.42966499686971815 2307 74-75 0.3753446613571185 2307 76-77 0.13410787406601443 2307 78-79 0.5330668349431136 2307 80-81 0.6731770835329272 2307 82-83 0.6944940849186381 2307 84-85 0.5811649845882698 2307 86-87 0.5916522818156906 2307 88-89 0.6204034439966719 2307 90-91 0.696194451322711 2307 92-93 0.7092988193502272 2307 94-95 0.7126111445843932 2307 96-97 0.706645525529165 2307 98-99 0.6788082269219977 2307 100-101 0.606108319183722 2201 1 0.12994986987460777 2201 2 0.10726479870998418 2201 3 0.1211696086635996 2201 4 0.14404921038094898 2201 5 0.15974233929205894 2201 6 0.14079077783735272 2201 7 0.22551331072552472 2201 8 0.22154036519678755 2201 9 0.2214762174480498 2201 10-11 0.23228365273667606 2201 12-13 0.2794097198447574 2201 14-15 0.44085329882837243 2201 16-17 0.432889459820359 2201 18-19 0.4490268946759528 2201 20-21 0.3610065187387548 2201 22-23 0.4374372401338249 2201 24-25 0.4321838692198767 2201 26-27 0.5698054541459072 2201 28-29 0.4863853048619333 2201 30-31 0.543713508354287 2201 32-33 0.58364649945716 2201 34-35 0.5835968150505195 2201 36-37 0.6067812998483575 2201 38-39 0.5558195064170519 2201 40-41 0.5900983532765665 2201 42-43 0.49571909663531954 2201 44-45 0.5764110735156436 2201 46-47 0.6140765016528107 2201 48-49 0.7202057547691965 2201 50-51 0.6850981992646084 2201 52-53 0.6510722300480225 2201 54-55 0.5808185728775399 2201 56-57 0.4846577800884262 2201 58-59 0.4785129543318405 2201 60-61 0.6425267950378455 2201 62-63 0.6102682681084133 2201 64-65 0.6019658032156983 2201 66-67 0.5463555890534053 2201 68-69 0.7560211206902423 2201 70-71 0.6073682076290012 2201 72-73 0.4541441681024523 2201 74-75 0.3859200864009651 2201 76-77 0.22050553789796723 2201 78-79 0.49788209333758715 2201 80-81 0.5137192765524148 2201 82-83 0.4854668010618326 2201 84-85 0.4031380522539365 2201 86-87 0.4432641240790929 2201 88-89 0.4953770515307383 2201 90-91 0.4769183500717915 2201 92-93 0.5436843351536105 2201 94-95 0.6416275575449646 2201 96-97 0.5185767017833278 2201 98-99 0.6094642108498043 2201 100-101 0.4354989070350719 1101 1 0.08955879919181342 1101 2 0.09750816047603195 1101 3 0.10049713892424705 1101 4 0.15385686926331488 1101 5 0.19941741330951857 1101 6 0.15147215238743428 1101 7 0.1077093760400274 1101 8 0.1542712443679335 1101 9 0.14534497557493609 1101 10-11 0.19265794352508436 1101 12-13 0.17442966472693655 1101 14-15 0.3911553581428251 1101 16-17 0.40292352205386095 1101 18-19 0.3414137394415988 1101 20-21 0.3293998738633235 1101 22-23 0.39770832536112266 1101 24-25 0.3570662099644295 1101 26-27 0.39772439796259107 1101 28-29 0.36295517942513555 1101 30-31 0.4160545939112126 1101 32-33 0.423070056166587 1101 34-35 0.46279342508547927 1101 36-37 0.40544251492941186 1101 38-39 0.5069875196373061 1101 40-41 0.482772434978628 1101 42-43 0.5748605567645271 1101 44-45 0.45383681169413137 1101 46-47 0.4935140885230922 1101 48-49 0.5310197674816166 1101 50-51 0.44154880555911546 1101 52-53 0.49085564791297287 1101 54-55 0.4729129803262637 1101 56-57 0.5556435171264553 1101 58-59 0.5037646566285332 1101 60-61 0.4690955286220486 1101 62-63 0.5188753583537533 1101 64-65 0.4106169233491883 1101 66-67 0.45850532855055803 1101 68-69 0.5950772355899829 1101 70-71 0.5966293701681096 1101 72-73 0.5996984973571173 1101 74-75 0.4382496783408243 1101 76-77 0.29654945994938586 1101 78-79 0.5887364144320557 1101 80-81 0.5478206810742847 1101 82-83 0.45529574580010745 1101 84-85 0.4825764515219788 1101 86-87 0.5234760346683629 1101 88-89 0.56422390570674 1101 90-91 0.5823780044591658 1101 92-93 0.6693581272384641 1101 94-95 0.7272260274222688 1101 96-97 0.5765394700602222 1101 98-99 0.5982323203986972 1101 100-101 0.5766731964160137 2304 1 0.04203260051207991 2304 2 0.0973987058304644 2304 3 0.09327809019285382 2304 4 0.09706473417072914 2304 5 0.13719702380638665 2304 6 0.1875231088641982 2304 7 0.08973702811631767 2304 8 0.16621553023715308 2304 9 0.13775770921186137 2304 10-11 0.1831183466623756 2304 12-13 0.17158708563034253 2304 14-15 0.26441831983012065 2304 16-17 0.27630926267828926 2304 18-19 0.2909679954086286 2304 20-21 0.3464356361188692 2304 22-23 0.42357506576030346 2304 24-25 0.40757604884538523 2304 26-27 0.37796113653833174 2304 28-29 0.397472888934125 2304 30-31 0.3570724601415236 2304 32-33 0.41589796424089087 2304 34-35 0.5061345567444917 2304 36-37 0.44424318567282484 2304 38-39 0.47358521303319634 2304 40-41 0.3485341542174538 2304 42-43 0.4554144563842186 2304 44-45 0.45362250273979754 2304 46-47 0.4499949977212694 2304 48-49 0.5409247931708023 2304 50-51 0.40117822816917226 2304 52-53 0.47646238585705447 2304 54-55 0.44320307565811135 2304 56-57 0.5104222302001062 2304 58-59 0.4505961567792909 2304 60-61 0.4483586643117192 2304 62-63 0.39276210664262834 2304 64-65 0.4285726576213449 2304 66-67 0.49639034158285966 2304 68-69 0.6382039867868485 2304 70-71 0.5413811128734167 2304 72-73 0.3755887757490015 2304 74-75 0.4446288914925418 2304 76-77 0.23998853349399596 2304 78-79 0.5396362331597118 2304 80-81 0.5142430849528736 2304 82-83 0.5719612405329642 2304 84-85 0.4743917134372637 2304 86-87 0.4817349382865359 2304 88-89 0.5712166560049852 2304 90-91 0.5816250270520626 2304 92-93 0.6008020728496142 2304 94-95 0.6145278107082675 2304 96-97 0.42968668441326585 2304 98-99 0.45856905075102716 2304 100-101 0.44867685761670373 2202 1 0.06394437074919779 2202 2 0.0810626371193095 2202 3 0.07129472370901624 2202 4 0.08568216993654687 2202 5 0.11496302620915344 2202 6 0.11462737698285252 2202 7 0.10621487332593915 2202 8 0.09112403262332691 2202 9 0.08962237549060603 2202 10-11 0.11763981779267141 2202 12-13 0.10780352488968248 2202 14-15 0.16217887950213594 2202 16-17 0.17018784995778447 2202 18-19 0.1938685947727592 2202 20-21 0.11466794337208341 2202 22-23 0.16406869488909592 2202 24-25 0.2514043240528565 2202 26-27 0.20192826775459594 2202 28-29 0.14845614258087636 2202 30-31 0.1656449380663787 2202 32-33 0.16590477871678644 2202 34-35 0.21670418497944155 2202 36-37 0.26514904634334613 2202 38-39 0.31167213742570254 2202 40-41 0.22983755548167295 2202 42-43 0.21494492129519926 2202 44-45 0.254897827478338 2202 46-47 0.3184071805881743 2202 48-49 0.2964217299753855 2202 50-51 0.23742106246015027 2202 52-53 0.30709785785906973 2202 54-55 0.2931934734420807 2202 56-57 0.38799393154021544 2202 58-59 0.41781950122670963 2202 60-61 0.24821730818676713 2202 62-63 0.29131495976190536 2202 64-65 0.325431029359855 2202 66-67 0.19423860444463514 2202 68-69 0.26279800553550814 2202 70-71 0.18771335397310907 2202 72-73 0.1469145895589321 2202 74-75 0.05889061604169399 2202 76-77 0.008902177711977544 2202 78-79 0.22296895437020936 2202 80-81 0.19208645820693704 2202 82-83 0.3303008979064188 2202 84-85 0.2526949310646387 2202 86-87 0.27443340436571617 2202 88-89 0.3427637854070156 2202 90-91 0.2465404324138163 2202 92-93 0.2489254591131349 2202 94-95 0.35817968391476995 2202 96-97 0.3174683512606187 2202 98-99 0.3840499248624347 2202 100-101 0.20516589803672147 1102 1 0.05572286831999662 1102 2 0.01860804351039036 1102 3 0.06601103704345235 1102 4 0.04849231148065769 1102 5 0.08964465860696436 1102 6 0.044941596870920364 1102 7 0.04252660914119133 1102 8 0.15197797717607386 1102 9 0.03315299754832779 1102 10-11 0.04014842486892434 1102 12-13 0.03100875328730268 1102 14-15 0.0576497330615382 1102 16-17 0.10787719424622111 1102 18-19 0.1496032081614871 1102 20-21 0.14051549137519714 1102 22-23 0.12453613378019668 1102 24-25 0.12788577033157367 1102 26-27 0.21544236107837378 1102 28-29 0.15759461493821192 1102 30-31 0.18525925656361153 1102 32-33 0.13646323539439464 1102 34-35 0.10902354198642428 1102 36-37 0.15094089942936506 1102 38-39 0.1032613062387071 1102 40-41 0.1624494598974664 1102 42-43 0.0943156035206627 1102 44-45 0.1964826891293967 1102 46-47 0.11302776310697737 1102 48-49 0.016106391881812954 1102 50-51 0.01745653856513485 1102 52-53 0.08280444984617219 1102 54-55 0.13774371255168916 1102 56-57 0.10485013403977916 1102 58-59 0.023597393309941594 1102 60-61 -0.19266621083080793 1102 62-63 0.0121512283846954 1102 64-65 0.0034598442732374224 1102 66-67 0.016954328029200383 1102 68-69 0.12054921972030996 1102 70-71 0.1334416225809818 1102 72-73 0.07991256477867381 1102 74-75 -0.05536522026628177 1102 76-77 -0.0419215048866306 1102 78-79 0.1699857270386147 1102 80-81 0.1414191380112797 1102 82-83 0.13011618692319615 1102 84-85 0.05725971512879369 1102 86-87 0.14587190948233797 1102 88-89 0.17481437136741107 1102 90-91 0.24836074672895592 1102 92-93 0.11879503907787736 1102 94-95 0.24587766323459448 1102 96-97 0.20400024931506877 1102 98-99 0.0401888523708287 1102 100-101 -0.016861300862633044 2305 1 0.02314182529205766 2305 2 0.026651025552212104 2305 3 0.03950664524596448 2305 4 0.0323641694093908 2305 5 0.015365620427751026 2305 6 0.016433343986584248 2305 7 0.0010175434881887213 2305 8 -0.033745529267108054 2305 9 -0.05273323691596943 2305 10-11 0.006716618134738894 2305 12-13 -0.03014212481075873 2305 14-15 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0.12558603908285448 1213 74-75 0.2477571967565524 1213 76-77 -1.9929825244123798 1213 78-79 -1.787621257539321 1213 80-81 -1.2165911544388521 1213 82-83 -0.8971284851347505 1213 84-85 -0.5974713644985457 1213 86-87 -0.38274986581993886 1213 88-89 -0.3801827419284578 1213 90-91 -1.544218346887483 1213 92-93 -0.7541835411621172 1213 94-95 -0.42947985195694827 1213 96-97 -0.4324831220230756 1213 98-99 -2.2363586494878795 1213 100-101 -0.6965384884236379 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 92.0 9 264.0 10 218.0 11 104.0 12 110.0 13 280.0 14 811.0 15 2100.0 16 4348.0 17 6956.0 18 8944.0 19 10643.0 20 13015.0 21 16620.0 22 20575.0 23 25656.0 24 32080.0 25 40025.0 26 51348.0 27 66883.0 28 94041.0 29 127846.0 30 130357.0 31 136966.0 32 179243.0 33 252461.0 34 383276.0 35 644450.0 36 1169452.0 37 1535242.0 38 302655.0 39 49.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.54316080393967 24.933173982403815 24.25254708690393 14.271118126752588 2 30.84124950159338 23.261055495865126 32.733805969922116 13.163889032619384 3 36.728755838158165 18.017488651817033 28.782735582153272 16.471019927871534 4 29.880451563176464 17.66128044349792 33.71095482770401 18.747313165621605 5 31.476500517780686 16.618001876336777 35.5375788559824 16.367918749900134 6 26.892159122986826 17.449015772145792 39.44274769664584 16.21607740822154 7 33.07113634167479 20.175891115965484 29.272285412762432 17.480687129597293 8 29.75566831700118 28.629309301906215 24.47358970203435 17.14143267905825 9 30.520688529604445 25.15417198326991 25.385001966857875 18.940137520267765 10-11 24.985158181166252 27.303049872505873 29.38995892225854 18.32183302406933 12-13 23.997577759364642 26.165952967368423 28.77822174743719 21.058247525829753 14-15 23.238894028483873 26.884324683374373 29.549046430499764 20.327734857641993 16-17 23.21825312585836 26.77197916119789 30.55917921537208 19.450588497571673 18-19 20.961822307655652 26.99279233883911 31.850847075900667 20.19453827760457 20-21 22.44340813480243 27.275915159566576 31.142084367170135 19.13859233846086 22-23 21.036771872638916 29.848257105226516 30.098508383684138 19.016462638450434 24-25 21.544017670525058 26.676936556088926 31.530709993844535 20.24833577954148 26-27 21.247666971776923 26.699077973550516 30.22908377901496 21.8241712756576 28-29 22.00885428436428 28.36076852783922 29.78239399701281 19.84798319078369 30-31 20.190937388484485 27.74069470789688 30.739239917856104 21.329127985762536 32-33 19.89046846217584 29.268671263864064 30.158702984678293 20.682157289281804 34-35 21.55791312194747 28.01581703732617 30.282164213230217 20.14410562749614 36-37 19.901059792401327 28.828533219126196 31.698634298339893 19.57177269013258 38-39 20.45198636397919 29.845263710229897 29.498409343316396 20.20434058247452 40-41 20.516856072008363 27.8408209527228 32.23270040972119 19.40962256554764 42-43 20.368060593771173 29.373086614107326 31.380850676102646 18.878002116018855 44-45 20.014869445809268 28.436336154856807 31.529055338420182 20.01973906091375 46-47 21.688474173308663 27.973327646761952 30.282942435449208 20.05525574448018 48-49 20.879783849530146 30.146939442467307 30.19967020446581 18.773606503536737 50-51 21.599967432082483 29.19527377120315 29.803924776480788 19.400834020233578 52-53 19.961505925625932 28.734981918095276 29.13455101882036 22.168961137458428 54-55 21.735980066644288 28.59249926918449 30.1525585106641 19.51896215350712 56-57 20.58108201813798 28.366290858518393 29.62072854598135 21.431898577362272 58-59 20.375611194611977 28.23241566941268 30.30804208443523 21.083931051540112 60-61 21.234015912083663 28.34215773820281 30.531296262969803 19.892530086743722 62-63 20.203400612309576 28.33119769411838 30.046199050452195 21.41920264311985 64-65 21.6872667156223 28.138509567275317 29.87474459997793 20.299479117124452 66-67 20.909007101368243 30.951702076467356 28.16061690371668 19.97867391844772 68-69 21.434348398864536 30.833073366272323 28.004470006365743 19.728108228497394 70-71 20.765897178791832 31.660995669377005 28.17563241776293 19.397474734068236 72-73 20.274925989770573 31.768500321614013 28.26241762595106 19.694156062664355 74-75 21.3339408400242 30.908764489134477 27.777057349348343 19.98023732149298 76-77 21.739893212522794 29.634709421743366 28.387609181922986 20.237788183810853 78-79 21.530430186818382 29.504275689715133 29.105282343155586 19.8600117803109 80-81 20.53093289850926 30.109157480171174 29.005427438352577 20.35448218296699 82-83 19.61043849897929 29.937307831898707 29.17680530541841 21.275448363703596 84-85 19.435988746026585 29.36551825156321 29.82245886764026 21.37603413476995 86-87 19.67242559291356 29.37580375749912 30.438566432442133 20.51320421714519 88-89 19.80401541596731 29.958914283762674 30.03895089911096 20.19811940115906 90-91 20.22543783392763 29.603066469018994 29.384698896479843 20.786796800573537 92-93 19.558581898204398 30.405740071755655 29.228412651144026 20.807265378895924 94-95 19.44463075675303 30.76123403759386 29.292195575598 20.50193963005511 96-97 20.16767099661663 31.101963290787342 28.47154104598099 20.258824666615038 98-99 20.45639388734604 30.33937157021434 28.241738543340844 20.962495999098774 100-101 20.221255679159285 30.343380495514467 28.335096012837035 21.10026781248921 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4551.0 1 2731.0 2 1042.5 3 1663.5 4 1744.0 5 1164.5 6 1010.5 7 1081.0 8 1147.5 9 1137.0 10 1077.5 11 1025.0 12 954.5 13 897.5 14 914.0 15 908.0 16 951.5 17 1039.5 18 1130.5 19 1259.5 20 1460.5 21 1758.0 22 2139.0 23 2786.5 24 3573.5 25 4509.5 26 5806.5 27 7785.0 28 10114.0 29 12662.0 30 15999.5 31 20876.0 32 32399.0 33 53658.5 34 82913.5 35 124539.5 36 179148.0 37 267355.5 38 374823.5 39 435432.0 40 433749.0 41 381106.0 42 333566.0 43 325089.0 44 316567.0 45 304971.5 46 300423.0 47 267373.0 48 209149.0 49 166500.5 50 137839.0 51 114456.5 52 92038.0 53 65967.5 54 43554.0 55 30118.0 56 20641.5 57 14172.5 58 10105.0 59 7048.5 60 4931.5 61 3764.5 62 2718.0 63 1783.0 64 1260.5 65 927.5 66 636.5 67 462.0 68 358.5 69 258.5 70 190.5 71 140.5 72 93.5 73 67.5 74 50.0 75 36.5 76 27.0 77 22.5 78 19.0 79 11.0 80 7.0 81 7.0 82 5.5 83 2.5 84 1.5 85 2.0 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17424002057401813 2 0.006923948415823429 3 0.0020353364848711727 4 0.0 5 0.0012173975236612622 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.0924019937927944E-4 12-13 0.0 14-15 9.510918153603611E-6 16-17 0.0 18-19 2.8532754460810833E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.8532754460810833E-5 38-39 1.9972928122567583E-4 40-41 8.55982633824325E-5 42-43 0.0014076158867333344 44-45 5.13589580294595E-4 46-47 0.005459267020168472 48-49 0.004232358578353607 50-51 0.007779931049647753 52-53 0.0038614327703630656 54-55 0.019050369061668033 56-57 0.01098511046741217 58-59 0.019326185688122537 60-61 0.00916852510007388 62-63 0.005668507219547752 64-65 0.012478324617527937 66-67 0.030044990447233806 68-69 0.03124336613458786 70-71 0.035523279303709486 72-73 0.0302922743192275 74-75 0.03339283363730228 76-77 0.03280315671177886 78-79 0.016891390640800013 80-81 0.0023587077020936955 82-83 0.0 84-85 0.00582068191000541 86-87 0.025356107797507226 88-89 0.026563994403014882 90-91 0.024709365363062178 92-93 0.033877890463136065 94-95 0.030501514518606783 96-97 0.032432230903788314 98-99 0.03899476442977481 100-101 0.02637377603994281 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5257116.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.49407958061634 #Duplication Level Percentage of deduplicated Percentage of total 1 85.31325753184976 27.721757795215417 2 6.1931494673521765 4.024813832935867 3 2.2901380932664734 2.2324778835960526 4 1.3128605561363962 1.7064078155739313 5 0.8447768636083184 1.3725123316976084 6 0.5167387131035117 1.007456931958247 7 0.4433358883559909 1.0084054146027968 8 0.3255310288535971 0.8462264926022957 9 0.27218538425140276 0.7959972182891146 >10 1.9096375529758747 12.567906702808884 >50 0.259003839914028 5.866871383632906 >100 0.2562504081193043 17.705554744702603 >500 0.044304432215973494 9.948111504559625 >1k 0.018360361795460984 9.599556359613292 >5k 2.3494062632069875E-4 0.5376737746097705 >10k+ 2.3493757550278497E-4 3.0582698136014894 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 121174 2.304951992689528 TruSeq Adapter, Index 2 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14391 0.2737432462970191 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 12775 0.24300395882457226 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 11903 0.22641691756468754 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 8732 0.16609867463453346 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 8102 0.1541149177609929 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5949 0.11316090419157575 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 5389 0.10250867585953971 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014837032319621633 0.0 0.0 2 0.0017690307765702716 0.0 0.0 3 0.0019402273033351366 0.0 0.0 4 0.00205435832117838 0.0 0.0 5 0.0023396858657864883 0.0 1.9021836307207222E-5 6 0.002586969737780182 0.0 1.9021836307207222E-5 7 0.0030434938091531555 0.0 1.9021836307207222E-5 8 0.0035380615531405432 0.0 1.9021836307207222E-5 9 0.00410871664235676 0.0 1.9021836307207222E-5 10-11 0.006819328316133789 0.0 1.9021836307207222E-5 12-13 0.0097486911074437 0.0 1.9021836307207222E-5 14-15 0.014161757130715776 0.0 1.9021836307207222E-5 16-17 0.020457984948401366 0.0 1.9021836307207222E-5 18-19 0.028627863642346867 0.0 1.9021836307207222E-5 20-21 0.03431539269820183 0.0 1.9021836307207222E-5 22-23 0.04026922746235769 0.0 2.8532754460810833E-5 24-25 0.047193175878181115 0.0 3.8043672614414444E-5 26-27 0.05565789303488833 0.0 3.8043672614414444E-5 28-29 0.06386581540144824 0.0 3.8043672614414444E-5 30-31 0.09262683189794557 0.0 3.8043672614414444E-5 32-33 0.10289862350383747 0.0 3.8043672614414444E-5 34-35 0.112770956547278 0.0 3.8043672614414444E-5 36-37 0.1232900320251636 0.0 3.8043672614414444E-5 38-39 0.1348838412544064 0.0 3.8043672614414444E-5 40-41 0.146411074056574 0.0 3.8043672614414444E-5 42-43 0.15845189643903615 0.0 3.8043672614414444E-5 44-45 0.1717766927722348 0.0 3.8043672614414444E-5 46-47 0.18581480796695377 0.0 3.8043672614414444E-5 48-49 0.20140320282071006 0.0 3.8043672614414444E-5 50-51 0.21710572869230962 0.0 5.7065508921621665E-5 52-53 0.23390201015157358 0.0 5.7065508921621665E-5 54-55 0.25118334843667134 0.0 5.7065508921621665E-5 56-57 0.2698818135266561 0.0 5.7065508921621665E-5 58-59 0.28964550144984436 0.0 5.7065508921621665E-5 60-61 0.31084533801422676 0.0 5.7065508921621665E-5 62-63 0.33311990832996646 0.0 5.7065508921621665E-5 64-65 0.35697329105920433 0.0 5.7065508921621665E-5 66-67 0.381501948977348 0.0 5.7065508921621665E-5 68-69 0.40825616174343504 0.0 5.7065508921621665E-5 70-71 0.43711228742146835 0.0 5.7065508921621665E-5 72-73 0.4667673302244044 0.0 5.7065508921621665E-5 74-75 0.4978204779959202 0.0 6.657642707522528E-5 76-77 0.5322690235482725 1.9021836307207222E-5 8.55982633824325E-5 78-79 0.5677827919338283 1.9021836307207222E-5 1.0462009968963972E-4 80-81 0.6070533729900577 1.9021836307207222E-5 1.1413101784324333E-4 82-83 0.649995168453578 1.9021836307207222E-5 1.1413101784324333E-4 84-85 0.6970171478049942 1.9021836307207222E-5 1.1413101784324333E-4 86-87 0.746331258431429 1.9021836307207222E-5 1.3315285415045055E-4 88-89 0.8009524613875745 1.9021836307207222E-5 1.3315285415045055E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 19430 0.0 70.39393 3 CGGAAGA 19435 0.0 70.22787 4 GATCGGA 19725 0.0 69.39894 1 GAAGAGC 19860 0.0 68.820656 6 ATCGGAA 20055 0.0 68.132996 2 GGAAGAG 21090 0.0 64.85197 5 GCGCGAC 45 1.5178202E-5 52.7713 2 GGTTCGC 60 1.4951638E-6 47.491463 3 GCCGTCT 15435 0.0 43.99241 50-51 CGTCTTC 15490 0.0 43.883244 52-53 ATGCCGT 15500 0.0 43.51534 48-49 TATGCCG 15430 0.0 43.481876 48-49 TCGTATG 15645 0.0 43.472637 44-45 TGCCGTC 15580 0.0 43.323833 50-51 CGTATGC 15540 0.0 43.29429 46-47 CTCGTAT 15620 0.0 43.146935 44-45 TCTCGTA 15725 0.0 43.084538 42-43 TGCCGGC 200 0.0 42.744755 2 CTGCTTG 15955 0.0 42.709305 58-59 ATCTCGT 15835 0.0 42.650272 42-43 >>END_MODULE