FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34298-1.341000000357c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34298-1.341000000357c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5257116
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1211742.304951992689528TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143910.2737432462970191No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC127750.24300395882457226No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG119030.22641691756468754No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA87320.16609867463453346No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA81020.1541149177609929No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA59490.11316090419157575No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT53890.10250867585953971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG194300.070.393933
CGGAAGA194350.070.227874
GATCGGA197250.069.398941
GAAGAGC198600.068.8206566
ATCGGAA200550.068.1329962
GGAAGAG210900.064.851975
GCGCGAC451.5178202E-552.77132
GGTTCGC601.4951638E-647.4914633
GCCGTCT154350.043.9924150-51
CGTCTTC154900.043.88324452-53
ATGCCGT155000.043.5153448-49
TATGCCG154300.043.48187648-49
TCGTATG156450.043.47263744-45
TGCCGTC155800.043.32383350-51
CGTATGC155400.043.2942946-47
CTCGTAT156200.043.14693544-45
TCTCGTA157250.043.08453842-43
TGCCGGC2000.042.7447552
CTGCTTG159550.042.70930558-59
ATCTCGT158350.042.65027242-43