##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34274-2.3410000003583d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4522654 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.698658796361606 33.0 33.0 33.0 33.0 33.0 2 32.72785559098706 33.0 33.0 33.0 33.0 33.0 3 32.74905420578271 33.0 33.0 33.0 33.0 33.0 4 36.69779403863307 37.0 37.0 37.0 37.0 37.0 5 36.66466592403487 37.0 37.0 37.0 37.0 37.0 6 36.54685279926344 37.0 37.0 37.0 37.0 37.0 7 36.49765027348986 37.0 37.0 37.0 37.0 37.0 8 36.442236571712094 37.0 37.0 37.0 37.0 37.0 9 36.45369422467427 37.0 37.0 37.0 37.0 37.0 10-11 36.409270198427734 37.0 37.0 37.0 37.0 37.0 12-13 36.39156179093072 37.0 37.0 37.0 37.0 37.0 14-15 38.2848953291585 40.0 37.0 40.0 37.0 40.0 16-17 38.260677027249926 40.0 37.0 40.0 37.0 40.0 18-19 38.25516356104181 40.0 37.0 40.0 37.0 40.0 20-21 38.17457260714616 40.0 37.0 40.0 37.0 40.0 22-23 38.081871838968894 40.0 37.0 40.0 37.0 40.0 24-25 38.05296038564967 40.0 37.0 40.0 35.0 40.0 26-27 37.97258810866363 40.0 37.0 40.0 35.0 40.0 28-29 37.84880592236328 40.0 37.0 40.0 33.0 40.0 30-31 37.78983413279017 40.0 37.0 40.0 33.0 40.0 32-33 37.67370795997218 40.0 37.0 40.0 33.0 40.0 34-35 37.577216607770566 40.0 37.0 40.0 33.0 40.0 36-37 37.49020552976195 40.0 37.0 40.0 33.0 40.0 38-39 37.424494002857614 40.0 37.0 40.0 33.0 40.0 40-41 37.34487239129945 40.0 37.0 40.0 33.0 40.0 42-43 37.207513110664664 40.0 37.0 40.0 33.0 40.0 44-45 37.111284657194645 40.0 37.0 40.0 33.0 40.0 46-47 37.02071504917245 40.0 37.0 40.0 33.0 40.0 48-49 36.814125069041324 37.0 37.0 40.0 33.0 40.0 50-51 36.65991373649189 37.0 37.0 40.0 33.0 40.0 52-53 36.543320691788495 37.0 37.0 40.0 33.0 40.0 54-55 36.36322654795171 37.0 37.0 40.0 33.0 40.0 56-57 36.182912068886985 37.0 37.0 40.0 33.0 40.0 58-59 35.978653684318985 37.0 37.0 40.0 33.0 40.0 60-61 35.70873816568766 37.0 37.0 40.0 27.0 40.0 62-63 35.47013136534433 37.0 33.0 40.0 27.0 40.0 64-65 35.18495666482556 37.0 33.0 38.5 27.0 40.0 66-67 34.85970560648681 37.0 33.0 37.0 27.0 40.0 68-69 34.59828388375498 37.0 33.0 37.0 27.0 40.0 70-71 34.31307082080566 37.0 33.0 37.0 27.0 40.0 72-73 33.96214026100604 37.0 33.0 37.0 27.0 38.5 74-75 33.54697507260118 37.0 33.0 37.0 27.0 37.0 76-77 30.84905577565739 33.0 30.0 35.0 24.5 37.0 78-79 33.25933301552584 35.0 33.0 37.0 27.0 37.0 80-81 33.65596715999057 37.0 33.0 37.0 27.0 37.0 82-83 33.590242919312416 37.0 33.0 37.0 27.0 37.0 84-85 33.451807168976444 37.0 33.0 37.0 27.0 37.0 86-87 33.283215673805685 37.0 33.0 37.0 27.0 37.0 88-89 33.12871314055862 37.0 33.0 37.0 27.0 37.0 90-91 32.9721195784599 37.0 33.0 37.0 27.0 37.0 92-93 32.81260991886623 33.0 33.0 37.0 27.0 37.0 94-95 32.72044390307107 33.0 33.0 37.0 27.0 37.0 96-97 32.58848477022562 33.0 33.0 37.0 27.0 37.0 98-99 32.28549674593723 33.0 33.0 37.0 22.0 37.0 100-101 30.673844494847494 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.081983135008727 2306 2 0.1219851511216774 2306 3 0.1261184016039678 2306 4 0.1464918334499643 2306 5 0.1837067380156867 2306 6 0.16735522455295637 2306 7 0.2405843959133378 2306 8 0.2418966887390397 2306 9 0.2453878916776162 2306 10-11 0.22226741234307923 2306 12-13 0.17391362973200586 2306 14-15 0.4194141522300967 2306 16-17 0.4364073001285007 2306 18-19 0.46805133346541794 2306 20-21 0.4692752159717628 2306 22-23 0.4673508184496029 2306 24-25 0.4497471539728153 2306 26-27 0.4678613556004976 2306 28-29 0.49951780290285797 2306 30-31 0.43004665792656027 2306 32-33 0.5407333850827811 2306 34-35 0.5296561669617006 2306 36-37 0.4819059053400281 2306 38-39 0.5652124225780142 2306 40-41 0.6248513190280605 2306 42-43 0.6315591403170728 2306 44-45 0.643688385546632 2306 46-47 0.6508137675996153 2306 48-49 0.6734312773936963 2306 50-51 0.5747129655256344 2306 52-53 0.5798952088983 2306 54-55 0.5842523497378451 2306 56-57 0.6579499125802286 2306 58-59 0.5524984953425474 2306 60-61 0.6122306168017673 2306 62-63 0.6159983592288256 2306 64-65 0.6258428424520233 2306 66-67 0.642837458086305 2306 68-69 0.6222571362032241 2306 70-71 0.5082221092391208 2306 72-73 0.5137757421274145 2306 74-75 0.6299741311943521 2306 76-77 0.08736808280163899 2306 78-79 0.49386536748426124 2306 80-81 0.5415317489662002 2306 82-83 0.5606520375598762 2306 84-85 0.6251527196596598 2306 86-87 0.6535561968454928 2306 88-89 0.6285172966195418 2306 90-91 0.8333194353203837 2306 92-93 0.6919493327656809 2306 94-95 0.7669702625001449 2306 96-97 0.687868954122969 2306 98-99 0.7245466341363667 2306 100-101 0.5642541783577322 2307 1 0.16528649622746627 2307 2 0.056276717260253406 2307 3 0.11112910407904764 2307 4 0.11211997444656419 2307 5 0.20258042159181144 2307 6 0.21224486560690536 2307 7 0.264818065647205 2307 8 0.2626571574316259 2307 9 0.2356472784578756 2307 10-11 0.22433135695849415 2307 12-13 0.2167509909935248 2307 14-15 0.4502241089341652 2307 16-17 0.4645690989997391 2307 18-19 0.5156973964904594 2307 20-21 0.4244318696839926 2307 22-23 0.4469746813723816 2307 24-25 0.4902135184557608 2307 26-27 0.5012999072571915 2307 28-29 0.5327952341921858 2307 30-31 0.6186579753322263 2307 32-33 0.6614718364220025 2307 34-35 0.6147898118106241 2307 36-37 0.6213892411683375 2307 38-39 0.6299862124984443 2307 40-41 0.655093709189174 2307 42-43 0.6054708852385033 2307 44-45 0.7608185183117584 2307 46-47 0.7243434652668412 2307 48-49 0.7338901763899415 2307 50-51 0.6954056081329583 2307 52-53 0.8357844863986088 2307 54-55 0.6886357471952991 2307 56-57 0.6383720868254628 2307 58-59 0.6600428168095149 2307 60-61 0.7245937252437926 2307 62-63 0.6751080270341063 2307 64-65 0.7000543247205187 2307 66-67 0.6762619147485651 2307 68-69 0.7463172673936 2307 70-71 0.6592313837454569 2307 72-73 0.5623264364760061 2307 74-75 0.5707660414452036 2307 76-77 0.3005057840927421 2307 78-79 0.5157991229545758 2307 80-81 0.6047802417738595 2307 82-83 0.6431908668393618 2307 84-85 0.5597945950132512 2307 86-87 0.7300691729889834 2307 88-89 0.7789453956227916 2307 90-91 0.68949070991615 2307 92-93 0.6999041344928472 2307 94-95 0.7346058466258825 2307 96-97 0.7399037852843762 2307 98-99 0.6689006897036975 2307 100-101 0.5445338230472245 2201 1 0.09875323103980804 2201 2 0.08274515874180821 2201 3 0.09604568205451613 2201 4 0.1346421045877335 2201 5 0.1891277067327266 2201 6 0.17727982053746416 2201 7 0.21836809450452677 2201 8 0.2474527740907675 2201 9 0.24026094122432795 2201 10-11 0.23549767587858383 2201 12-13 0.19738015084493554 2201 14-15 0.41440087841657913 2201 16-17 0.44139014478256655 2201 18-19 0.33762391599990593 2201 20-21 0.4037466073135292 2201 22-23 0.46897400473211803 2201 24-25 0.35327968347011307 2201 26-27 0.43443394684498315 2201 28-29 0.39342958134373873 2201 30-31 0.4547564754119051 2201 32-33 0.4649958771739904 2201 34-35 0.4719295947903319 2201 36-37 0.4772940730981574 2201 38-39 0.551204065893323 2201 40-41 0.5174703327748915 2201 42-43 0.5088456543592699 2201 44-45 0.5435039792569469 2201 46-47 0.537942076812584 2201 48-49 0.6212185805840349 2201 50-51 0.5461669522815242 2201 52-53 0.6111520973341698 2201 54-55 0.5125977091961076 2201 56-57 0.49114993455622624 2201 58-59 0.5299652281754206 2201 60-61 0.4448747064093368 2201 62-63 0.44557747872843123 2201 64-65 0.39563458193463674 2201 66-67 0.47279099583062845 2201 68-69 0.5487257702723127 2201 70-71 0.5322321677234001 2201 72-73 0.6513268212403247 2201 74-75 0.46012023805977975 2201 76-77 0.20528280656182218 2201 78-79 0.55062669321741 2201 80-81 0.5385946371613173 2201 82-83 0.530901197100917 2201 84-85 0.3681401104859745 2201 86-87 0.6239283013574237 2201 88-89 0.7311212956699364 2201 90-91 0.7040368242506005 2201 92-93 0.5942205317281619 2201 94-95 0.6918711527736079 2201 96-97 0.6913656832954089 2201 98-99 0.7249565065393639 2201 100-101 0.5667657285966641 1101 1 0.11585971588048949 1101 2 0.10935606511647222 1101 3 0.09378876406772463 1101 4 0.1313918369847329 1101 5 0.15360786089389222 1101 6 0.09320466696996732 1101 7 0.10013710841988654 1101 8 0.21054307583049336 1101 9 0.15343173994820347 1101 10-11 0.19220581096430323 1101 12-13 0.15336269853467854 1101 14-15 0.34526145236302597 1101 16-17 0.4025598007631501 1101 18-19 0.35126300291526746 1101 20-21 0.4078218154706832 1101 22-23 0.35764819961443095 1101 24-25 0.3784654361180557 1101 26-27 0.37431873539426164 1101 28-29 0.4360722388769531 1101 30-31 0.39204093160120834 1101 32-33 0.4272541871967874 1101 34-35 0.42261485408432975 1101 36-37 0.460537371422852 1101 38-39 0.544903783710339 1101 40-41 0.4647554303925574 1101 42-43 0.5661393543955242 1101 44-45 0.5750366427449691 1101 46-47 0.4938422396262041 1101 48-49 0.5610911319540293 1101 50-51 0.5468029671644814 1101 52-53 0.6231275192552843 1101 54-55 0.5996305753482076 1101 56-57 0.5617989008330113 1101 58-59 0.6381685964476489 1101 60-61 0.48641105924770045 1101 62-63 0.5840475310753632 1101 64-65 0.44856647816518347 1101 66-67 0.5698006204403683 1101 68-69 0.6230571721935974 1101 70-71 0.5733448139156181 1101 72-73 0.5853026443953482 1101 74-75 0.39640079063438804 1101 76-77 0.31285248001110233 1101 78-79 0.5854731302648588 1101 80-81 0.6205737302362095 1101 82-83 0.5815524288480702 1101 84-85 0.5996021258720745 1101 86-87 0.44246853779429074 1101 88-89 0.6252874820235235 1101 90-91 0.5904468680178567 1101 92-93 0.7345307101043872 1101 94-95 0.7125738671073449 1101 96-97 0.6233384303238054 1101 98-99 0.6303193934946663 1101 100-101 0.6619187138087845 2304 1 0.005254912254500255 2304 2 0.03750790010028027 2304 3 0.071137978171123 2304 4 0.08108594784977186 2304 5 0.1559944362115786 2304 6 0.09337793876345302 2304 7 0.1090020501595177 2304 8 0.13859712539795055 2304 9 0.09748369836132298 2304 10-11 0.11721951042032686 2304 12-13 0.16605745896247726 2304 14-15 0.2640376563149971 2304 16-17 0.3065523611429697 2304 18-19 0.2971753619099786 2304 20-21 0.24956958112759509 2304 22-23 0.31053322040001774 2304 24-25 0.2794743024638038 2304 26-27 0.42820514821801936 2304 28-29 0.3430351138672947 2304 30-31 0.38366743590884766 2304 32-33 0.3777955376678932 2304 34-35 0.38164660502553716 2304 36-37 0.37540909619985285 2304 38-39 0.5085468107801319 2304 40-41 0.5192364842616684 2304 42-43 0.5784710947240441 2304 44-45 0.45284133537130344 2304 46-47 0.4882491450605215 2304 48-49 0.57352586080804 2304 50-51 0.4974305818816873 2304 52-53 0.5014296456338485 2304 54-55 0.48135758638086656 2304 56-57 0.4977509397291442 2304 58-59 0.6338263953920702 2304 60-61 0.48392285812357727 2304 62-63 0.4878726008940575 2304 64-65 0.48827470762559955 2304 66-67 0.39729983687691117 2304 68-69 0.46360037678851995 2304 70-71 0.5965518782512333 2304 72-73 0.544757664136192 2304 74-75 0.435379222095186 2304 76-77 0.23882784617990538 2304 78-79 0.46591926796802596 2304 80-81 0.3312610403072398 2304 82-83 0.39010423426500296 2304 84-85 0.4323600751698322 2304 86-87 0.5582153346818401 2304 88-89 0.6586825231301461 2304 90-91 0.5602727992401739 2304 92-93 0.5230441446560832 2304 94-95 0.5428128921603204 2304 96-97 0.4348175214645167 2304 98-99 0.5151855724180265 2304 100-101 0.5288435813256527 2202 1 0.04927959832755846 2202 2 0.04034103249707499 2202 3 0.048622820566983194 2202 4 0.09887014200688782 2202 5 0.10796608959896048 2202 6 0.14096400658099384 2202 7 0.12492823655873053 2202 8 0.0858000305326101 2202 9 0.13793098600925902 2202 10-11 0.15417978468413907 2202 12-13 0.15731664671291412 2202 14-15 0.17293062703068074 2202 16-17 0.20571807408909848 2202 18-19 0.1360468993948345 2202 20-21 0.12686742877694002 2202 22-23 0.19160507122939663 2202 24-25 0.22916186798659766 2202 26-27 0.20714008263699668 2202 28-29 0.16566184221274227 2202 30-31 0.2797909623742285 2202 32-33 0.24254799258822146 2202 34-35 0.2501230474004359 2202 36-37 0.2748402856276755 2202 38-39 0.3118366369845873 2202 40-41 0.2178998558603027 2202 42-43 0.27596037082832225 2202 44-45 0.20163760901774452 2202 46-47 0.2264906809728302 2202 48-49 0.23845584607268933 2202 50-51 0.1286778517656586 2202 52-53 0.188813488297626 2202 54-55 0.20542494234722142 2202 56-57 0.2720876671427135 2202 58-59 0.36677769449048725 2202 60-61 0.2966959789301171 2202 62-63 0.35218437868737595 2202 64-65 0.38820885722721954 2202 66-67 0.42925216832318824 2202 68-69 0.3027344786313151 2202 70-71 0.24723371562659935 2202 72-73 0.19257142362971535 2202 74-75 0.20551979758908345 2202 76-77 0.13493000493724594 2202 78-79 0.26584206028701374 2202 80-81 0.37658158504910233 2202 82-83 0.3731660120583058 2202 84-85 0.2656187125288909 2202 86-87 0.273725269392024 2202 88-89 0.29470968014020826 2202 90-91 0.44887059709220267 2202 92-93 0.33550756110143 2202 94-95 0.41603737030774113 2202 96-97 0.3909723314193201 2202 98-99 0.3848314872908176 2202 100-101 0.3656439799953901 1102 1 0.07996101132674482 1102 2 0.06440187703660172 1102 3 0.08483729183838307 1102 4 0.10409248308268104 1102 5 0.11272744860466588 1102 6 0.09685553571709704 1102 7 0.11651022005506206 1102 8 0.14730420738672478 1102 9 0.13449590552841073 1102 10-11 0.09257978213728535 1102 12-13 0.16218433721821413 1102 14-15 0.23592535502579182 1102 16-17 0.23920644766285903 1102 18-19 0.22756648476566 1102 20-21 0.21163050691466623 1102 22-23 0.19547057143314817 1102 24-25 0.25881976071275403 1102 26-27 0.28079470264514583 1102 28-29 0.2991623133822614 1102 30-31 0.296945353164773 1102 32-33 0.18473740273492467 1102 34-35 0.2582459462390574 1102 36-37 0.2621499076733116 1102 38-39 0.20482512516714735 1102 40-41 0.2803536939817377 1102 42-43 0.2547338656327227 1102 44-45 0.230135724339668 1102 46-47 0.20875847080576193 1102 48-49 0.2819349235991737 1102 50-51 0.2845238557387688 1102 52-53 0.1973477053921613 1102 54-55 0.20236520569277872 1102 56-57 0.2059141366822459 1102 58-59 0.2990410273144377 1102 60-61 0.1856887585755942 1102 62-63 0.36650411177620157 1102 64-65 0.3217615390731865 1102 66-67 0.2063463258211513 1102 68-69 0.2841643507788234 1102 70-71 0.32864830660051325 1102 72-73 0.22663666150665307 1102 74-75 0.19028669602021608 1102 76-77 0.08702066049959711 1102 78-79 0.3701542164606053 1102 80-81 0.2243833245560367 1102 82-83 0.379615940746163 1102 84-85 0.25604378384102944 1102 86-87 0.21164327316137843 1102 88-89 0.20245628007908323 1102 90-91 0.36026589558445465 1102 92-93 0.30434829306964417 1102 94-95 0.21830153086193604 1102 96-97 0.24487635291483656 1102 98-99 0.21118005086050573 1102 100-101 0.1756133539766438 2305 1 0.104798291249935 2305 2 0.06413170030528903 2305 3 0.051465999790728745 2305 4 0.08303823561418966 2305 5 0.08087562156243422 2305 6 0.022317459777340787 2305 7 0.03489898427562821 2305 8 0.09911338669699177 2305 9 -0.046897067643712376 2305 10-11 0.038988217903316524 2305 12-13 0.033847040548529606 2305 14-15 0.10994903456492722 2305 16-17 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-1.051580598121312 1213 58-59 -1.4651226104953707 1213 60-61 -0.6622364600727053 1213 62-63 -0.4833184796341712 1213 64-65 -0.7737292394842328 1213 66-67 -0.6266048124641799 1213 68-69 -0.18887196437906084 1213 70-71 -0.10345158169777591 1213 72-73 -0.19168846999357214 1213 74-75 -0.09552684167305614 1213 76-77 -2.467475073740264 1213 78-79 -2.145817571077913 1213 80-81 -1.5111782791069501 1213 82-83 -1.1140429990213718 1213 84-85 -0.7684019440010488 1213 86-87 -0.4998965849809167 1213 88-89 -0.6488784342515785 1213 90-91 -1.8054717679478784 1213 92-93 -0.9760968192026702 1213 94-95 -0.5766974835347867 1213 96-97 -0.6093700409875638 1213 98-99 -2.458142787815259 1213 100-101 -0.9526432002851344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 2.0 8 39.0 9 141.0 10 240.0 11 214.0 12 160.0 13 220.0 14 536.0 15 1057.0 16 2099.0 17 3376.0 18 4828.0 19 6705.0 20 8879.0 21 11525.0 22 14583.0 23 18245.0 24 22788.0 25 28206.0 26 34886.0 27 43449.0 28 55064.0 29 71314.0 30 84634.0 31 102290.0 32 137407.0 33 195855.0 34 299861.0 35 510375.0 36 970341.0 37 1469322.0 38 423775.0 39 237.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.168398303588695 33.141752056113496 15.791235178717026 17.898614461580785 2 30.117088054081943 25.875993377739714 29.93138703272632 14.075531535452024 3 31.5689251298044 23.699402154613722 25.40458155024108 19.3270911653408 4 27.58581576216089 23.515970932111983 29.317431755778795 19.580781549948327 5 29.703060055304498 21.93484806741612 29.79906681955814 18.563025057721248 6 27.60746234401305 22.17505473556014 31.46508665044905 18.752396269977762 7 30.870325255922737 23.37651741654347 25.06216482622814 20.69099250130565 8 27.23175374459333 27.02216441938738 25.241042096078985 20.505039739940308 9 26.351562600190064 24.080705709523656 28.60291766736965 20.964814022916634 10-11 22.471124426841637 29.25748526164213 30.968256907611984 17.303133403904248 12-13 22.05154318681022 29.0021080542531 30.023521587103502 18.922827171833177 14-15 21.806775402230638 28.99507678456057 30.474097731110984 18.72405008209781 16-17 22.138129514218864 28.348310527402713 30.69140376424993 18.822156194128493 18-19 21.243535459879915 28.287232263095106 31.28060772287773 19.18862455414725 20-21 21.849162018584664 28.69024471029621 30.77791270347013 18.682680567648998 22-23 21.300811426211247 29.877678018260962 30.11688490872837 18.704625646799425 24-25 21.172579197966503 28.86641339355166 30.681630741595534 19.279376666886304 26-27 21.031268365875437 28.85587754446836 30.39889852285848 19.713955566797726 28-29 21.13715751857206 29.726516775327056 30.24019745927944 18.896128246821444 30-31 20.461647077136565 29.584608948639453 30.720037393972653 19.23370658025133 32-33 20.384590552361512 30.061806629470215 30.272844219345547 19.280758598822725 34-35 20.947700177815946 30.102943979353714 30.33925434048238 18.61010150234796 36-37 20.840853808594204 29.759866609266727 30.729938821306874 18.669340760832192 38-39 20.745933296083727 29.050877993244217 31.182717716167296 19.020470994504763 40-41 20.628275249596392 29.2921530655192 29.837872761144652 20.241698923739758 42-43 20.91241965334023 29.53939003977809 30.327776782772702 19.220413524108974 44-45 20.501141042391588 29.150576247966058 30.56403822652317 19.784244483119185 46-47 21.010711015090326 29.267819159315945 30.04921832995552 19.672251495638214 48-49 20.573986843039197 30.026989658091935 30.222701163721705 19.176322335147162 50-51 21.213153135861457 29.30639466925886 29.88502747939211 19.59542471548757 52-53 20.548484067870064 29.180159492666057 29.613584626967814 20.657771812496062 54-55 21.10907772025309 29.3956737797259 29.868081464395264 19.627167035625746 56-57 20.566612186355833 29.196430526104667 30.087306338195578 20.149650949343926 58-59 20.66609689942753 29.502306584239502 30.11325644313752 19.718340073195446 60-61 20.842134801583164 29.650494962694673 29.888265901878274 19.619104333843886 62-63 20.310500909921124 29.697603247854552 29.647695787828066 20.344200054396257 64-65 20.732938004699406 29.50879361300009 29.82326308991222 19.935005292388283 66-67 20.588915421857802 29.918122573174276 29.508796263477727 19.98416574149019 68-69 20.475396269541697 30.127489046718043 29.670778356799243 19.72633632694102 70-71 20.511716250348655 30.64221336123902 29.37053176737685 19.47553862103548 72-73 20.41924710253915 30.57798814474034 29.314662036627443 19.688102716093073 74-75 20.54700213069454 30.105411097085394 29.317189040360216 20.030397731859846 76-77 20.687434528570318 29.845682070737457 29.321280249570016 20.145603151122216 78-79 20.385990297940644 29.609570121434416 29.81727393826849 20.187165642356454 80-81 20.385510983532935 29.834652760626046 29.588076885062232 20.191759370778787 82-83 20.04649261252353 29.953485276565484 29.47931679053936 20.520705320371626 84-85 20.080417726394874 29.979064148880703 29.550042831372643 20.390475293351784 86-87 20.16011937792371 29.780043477308883 29.927629234852 20.132207909915405 88-89 20.34353937541995 30.137641787651276 29.54504537779887 19.9737734591299 90-91 20.60948554551629 29.86406630234554 29.142132789144416 20.384315362993753 92-93 20.230587319831884 30.139069265445713 29.186153878311323 20.444189536411084 94-95 19.778261353759866 30.494184204406 29.118768480386255 20.608785961447886 96-97 19.960923321723357 30.65886902577707 29.00331356606816 20.37689408643141 98-99 20.144950697850348 30.18213952140611 28.87028372445653 20.802626056287014 100-101 20.148089283968616 30.122413574220698 28.803594643598856 20.925902498211833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 69.0 1 44.0 2 34.0 3 80.0 4 89.0 5 58.5 6 59.0 7 73.5 8 78.5 9 77.5 10 73.0 11 72.0 12 89.0 13 111.5 14 138.5 15 161.5 16 190.5 17 271.5 18 397.5 19 597.5 20 862.0 21 1328.0 22 2175.0 23 3440.5 24 5407.5 25 8152.5 26 12314.0 27 17961.0 28 24680.0 29 31776.0 30 39214.0 31 48038.0 32 62049.0 33 84945.0 34 113571.0 35 150133.0 36 196971.5 37 253083.5 38 305290.5 39 320600.0 40 325104.0 41 312166.0 42 277468.5 43 264900.5 44 249258.5 45 233955.5 46 235906.0 47 218430.0 48 174214.0 49 131036.0 50 99297.5 51 79393.5 52 63423.0 53 48281.5 54 37461.0 55 28502.5 56 19441.0 57 12619.5 58 8263.5 59 5633.0 60 4093.5 61 2957.0 62 2033.5 63 1284.0 64 845.0 65 577.5 66 383.5 67 279.0 68 178.0 69 128.0 70 110.0 71 80.5 72 55.5 73 40.0 74 27.5 75 23.0 76 16.0 77 15.0 78 13.5 79 6.0 80 4.0 81 4.5 82 7.0 83 4.5 84 1.5 85 2.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 1.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17571540958030396 2 0.007318711535306482 3 0.001768872878623923 4 0.0 5 0.001238211015036746 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.6483003121618417E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.1055455491399518E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.2110910982799036E-5 38-39 2.4322002081078944E-4 40-41 5.5277277456997596E-5 42-43 0.0014150983028991383 44-45 7.738818843979663E-4 46-47 0.005605115934139556 48-49 0.004002074887886626 50-51 0.007871484309876458 52-53 0.004178962175749018 54-55 0.02043048174810631 56-57 0.01174089373186629 58-59 0.02044153720359771 60-61 0.009828299931854172 62-63 0.006279498719114927 64-65 0.013122825668291229 66-67 0.03193921091465321 68-69 0.03361964014934594 70-71 0.03783176869156915 72-73 0.032149264568989804 74-75 0.03522268119559887 76-77 0.035333235750512865 78-79 0.0183631115712146 80-81 0.002454311119090693 82-83 0.0 84-85 0.005925724143390142 86-87 0.02686475684410083 88-89 0.02830196605798277 90-91 0.02620142951461686 92-93 0.03611817309040223 94-95 0.03262464915511998 96-97 0.034117135646458914 98-99 0.03994336069042646 100-101 0.02785974783832679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4522654.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.01249043461333 #Duplication Level Percentage of deduplicated Percentage of total 1 89.77068347716349 43.101140817560136 2 4.677697177639531 4.491757819948715 3 1.545519779888054 2.2261276094514266 4 0.8501362667742339 1.6326863750646317 5 0.5336014738650908 1.2809767829921623 6 0.33384056247779287 0.9617110087590579 7 0.2568086092922626 0.8631014628019777 8 0.2105172334794137 0.808596532700129 9 0.182184839512832 0.787243307799727 >10 1.3319601263110894 12.837737423093085 >50 0.16532379576053657 5.645375531821324 >100 0.1120095429588326 10.492987141086967 >500 0.01850897604789687 6.286176933277772 >1k 0.011161461244659525 7.868213080888423 >5k 0.0 0.0 >10k+ 4.667758414073406E-5 0.716168172754403 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 31956 0.7065762713663261 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.402146173463634E-4 0.0 0.0 2 9.949909942259566E-4 0.0 0.0 3 0.0014150983028991383 0.0 0.0 4 0.002144758365331507 0.0 0.0 5 0.0025427547630218895 0.0 0.0 6 0.004864400416215788 0.0 0.0 7 0.006699606027788108 0.0 0.0 8 0.008402146173463635 0.0 0.0 9 0.009485580811620787 0.0 2.2110910982799036E-5 10-11 0.01334393477811922 0.0 2.2110910982799036E-5 12-13 0.02040837083712351 0.0 3.3166366474198556E-5 14-15 0.028954237931975338 0.0 4.422182196559807E-5 16-17 0.04079463076326423 0.0 4.422182196559807E-5 18-19 0.05605115934139556 0.0 4.422182196559807E-5 20-21 0.07170568431721727 0.0 4.422182196559807E-5 22-23 0.0897592431346727 0.0 4.422182196559807E-5 24-25 0.10954850846427783 0.0 4.422182196559807E-5 26-27 0.1308744821071875 2.2110910982799036E-5 4.422182196559807E-5 28-29 0.15297433763449514 2.2110910982799036E-5 4.422182196559807E-5 30-31 0.17627923781036534 2.2110910982799036E-5 7.738818843979663E-5 32-33 0.1999268571064689 2.2110910982799036E-5 8.844364393119615E-5 34-35 0.22536546019217918 2.2110910982799036E-5 8.844364393119615E-5 36-37 0.2529377661877296 2.2110910982799036E-5 8.844364393119615E-5 38-39 0.2854297498769528 2.2110910982799036E-5 8.844364393119615E-5 40-41 0.31933683186907513 2.2110910982799036E-5 8.844364393119615E-5 42-43 0.3563836632207549 2.2110910982799036E-5 8.844364393119615E-5 44-45 0.39860444774240966 2.2110910982799036E-5 8.844364393119615E-5 46-47 0.4429921015403787 2.2110910982799036E-5 8.844364393119615E-5 48-49 0.4914702738701656 2.2110910982799036E-5 8.844364393119615E-5 50-51 0.5416730972566108 2.2110910982799036E-5 8.844364393119615E-5 52-53 0.5952036127459672 2.2110910982799036E-5 8.844364393119615E-5 54-55 0.6507241102237757 2.2110910982799036E-5 8.844364393119615E-5 56-57 0.7107110117201094 2.2110910982799036E-5 8.844364393119615E-5 58-59 0.7736497198326469 2.2110910982799036E-5 8.844364393119615E-5 60-61 0.8405241701001227 2.2110910982799036E-5 8.844364393119615E-5 62-63 0.910516258816173 2.2110910982799036E-5 1.1055455491399519E-4 64-65 0.9826420504420634 2.2110910982799036E-5 1.1055455491399519E-4 66-67 1.0575317059408036 2.2110910982799036E-5 1.1055455491399519E-4 68-69 1.1378827564522955 2.2110910982799036E-5 1.2161001040539471E-4 70-71 1.2200026798424113 2.2110910982799036E-5 1.3266546589679423E-4 72-73 1.3054834617019122 2.2110910982799036E-5 1.3266546589679423E-4 74-75 1.3959723648990172 2.2110910982799036E-5 1.5477637687959326E-4 76-77 1.4908171175597338 2.2110910982799036E-5 1.5477637687959326E-4 78-79 1.5893322814435948 2.2110910982799036E-5 1.5477637687959326E-4 80-81 1.69546465416103 2.2110910982799036E-5 1.658318323709928E-4 82-83 1.8086725183929615 2.2110910982799036E-5 1.768872878623923E-4 84-85 1.9295749796469064 2.2110910982799036E-5 1.768872878623923E-4 86-87 2.0565468859656297 2.2110910982799036E-5 1.768872878623923E-4 88-89 2.186105768869341 2.2110910982799036E-5 1.768872878623923E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGG 45 1.5020039E-5 52.864574 1 CGAGGCG 95 7.2759576E-12 50.08223 1 CGCGTTG 55 4.9177135E-5 43.252834 1 CGGTGCG 45 9.55053E-4 42.29166 1 CGAGCGC 45 9.55053E-4 42.29166 1 CGACTGG 90 1.180706E-8 42.29166 1 CGGGTCG 70 4.2645825E-6 40.781242 1 CGACGGG 130 2.0190782E-10 36.598553 1 CGAGTGG 185 0.0 36.00506 1 CGGGGCG 175 0.0 35.343746 1 CGTCCGT 55 0.0025597122 34.60227 1 GGTACGG 70 2.0345481E-4 33.984367 1 CGGACGC 70 2.0345481E-4 33.984367 1 CGCGAGG 85 1.6149925E-5 33.584553 1 GTGTTTG 56920 0.0 32.02245 1 GTTGACG 150 9.404175E-10 31.718744 1 ACCGGCC 75 3.0503265E-4 31.718744 1 GGACGGG 360 0.0 31.718744 1 CGTCTTC 5860 0.0 31.291328 52-53 GCCGTCT 5895 0.0 31.22504 50-51 >>END_MODULE