FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34274-2.3410000003583d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34274-2.3410000003583d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522654
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT319560.7065762713663261TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG451.5020039E-552.8645741
CGAGGCG957.2759576E-1250.082231
CGCGTTG554.9177135E-543.2528341
CGGTGCG459.55053E-442.291661
CGAGCGC459.55053E-442.291661
CGACTGG901.180706E-842.291661
CGGGTCG704.2645825E-640.7812421
CGACGGG1302.0190782E-1036.5985531
CGAGTGG1850.036.005061
CGGGGCG1750.035.3437461
CGTCCGT550.002559712234.602271
GGTACGG702.0345481E-433.9843671
CGGACGC702.0345481E-433.9843671
CGCGAGG851.6149925E-533.5845531
GTGTTTG569200.032.022451
GTTGACG1509.404175E-1031.7187441
ACCGGCC753.0503265E-431.7187441
GGACGGG3600.031.7187441
CGTCTTC58600.031.29132852-53
GCCGTCT58950.031.2250450-51
Produced by FastQC (version 0.11.2)