FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34271-1.341000000358dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34271-1.341000000358dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5114626
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3429476.705221457052773TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG459050.081.0064243
GATCGGA461200.080.806611
ATCGGAA461850.080.506612
CGGAAGA462700.080.192924
GAAGAGC475900.077.958636
GGAAGAG520550.071.40865
GAGCACA532900.069.263559
AGAGCAC534950.069.078028
AAGAGCA553400.066.800757
ATGCCGT403600.045.47577348-49
GCCGTCT405500.045.46338350-51
TCGTATG407600.045.2359444-45
CGTATCT402500.045.126438-39
GTATGCC407700.045.10406546-47
TGCCGTC406500.045.03603450-51
TATGCCG405300.045.0271948-49
TATCTCG404100.045.01912340-41
TCTCGTA406400.044.96520642-43
CGTATGC406300.044.94383646-47
AATTCGT400500.044.84777534-35
Produced by FastQC (version 0.11.2)