##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34271-1.341000000358dd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5114626 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67492266296695 33.0 33.0 33.0 33.0 33.0 2 32.691115440307854 33.0 33.0 33.0 33.0 33.0 3 32.72805890401371 33.0 33.0 33.0 33.0 33.0 4 36.65873262287408 37.0 37.0 37.0 37.0 37.0 5 36.6324446792395 37.0 37.0 37.0 37.0 37.0 6 36.51429293168259 37.0 37.0 37.0 37.0 37.0 7 36.4047717271996 37.0 37.0 37.0 37.0 37.0 8 36.34670198759401 37.0 37.0 37.0 37.0 37.0 9 36.41077705388429 37.0 37.0 37.0 37.0 37.0 10-11 36.32894692593359 37.0 37.0 37.0 37.0 37.0 12-13 36.31127339516125 37.0 37.0 37.0 35.0 37.0 14-15 38.144620447321074 40.0 37.0 40.0 37.0 40.0 16-17 38.16328974982726 40.0 37.0 40.0 37.0 40.0 18-19 38.10384346773351 40.0 37.0 40.0 37.0 40.0 20-21 38.082524020329146 40.0 37.0 40.0 37.0 40.0 22-23 37.8901046723651 40.0 37.0 40.0 33.0 40.0 24-25 37.92531184098309 40.0 37.0 40.0 33.0 40.0 26-27 37.85716306529549 40.0 37.0 40.0 33.0 40.0 28-29 37.654857461718606 40.0 37.0 40.0 33.0 40.0 30-31 37.62813996565927 40.0 37.0 40.0 33.0 40.0 32-33 37.476411373969476 40.0 37.0 40.0 33.0 40.0 34-35 37.29375187941406 40.0 37.0 40.0 33.0 40.0 36-37 36.97069697764802 37.0 37.0 40.0 33.0 40.0 38-39 37.13782728199482 38.5 37.0 40.0 33.0 40.0 40-41 36.90029495802821 38.5 37.0 40.0 33.0 40.0 42-43 36.7103720780366 37.0 37.0 40.0 33.0 40.0 44-45 36.74216589834721 37.0 37.0 40.0 33.0 40.0 46-47 36.7047201496258 37.0 37.0 40.0 33.0 40.0 48-49 36.37039932147532 37.0 37.0 40.0 33.0 40.0 50-51 36.28635857245476 37.0 37.0 40.0 33.0 40.0 52-53 36.211819397938385 37.0 37.0 40.0 33.0 40.0 54-55 35.89734283601577 37.0 37.0 40.0 30.0 40.0 56-57 35.75617102795004 37.0 37.0 40.0 27.0 40.0 58-59 35.48337600833375 37.0 33.0 40.0 27.0 40.0 60-61 35.19905199324447 37.0 33.0 40.0 27.0 40.0 62-63 34.987580616842756 37.0 33.0 37.0 27.0 40.0 64-65 34.687179179865744 37.0 33.0 37.0 27.0 40.0 66-67 34.08588330798772 37.0 33.0 37.0 27.0 40.0 68-69 33.88317083204129 37.0 33.0 37.0 27.0 40.0 70-71 33.4821992849526 37.0 33.0 37.0 27.0 40.0 72-73 32.70548305975842 37.0 33.0 37.0 22.0 37.0 74-75 31.83971897456432 35.0 33.0 37.0 18.5 37.0 76-77 29.250067258094724 33.0 27.0 35.0 15.0 37.0 78-79 31.41416527816501 33.0 33.0 37.0 15.0 37.0 80-81 31.710202955211194 35.0 33.0 37.0 15.0 37.0 82-83 31.61514439178935 35.0 33.0 37.0 15.0 37.0 84-85 31.47365721364573 33.0 33.0 37.0 15.0 37.0 86-87 31.30299566380807 33.0 33.0 37.0 15.0 37.0 88-89 31.161309350869445 33.0 33.0 37.0 15.0 37.0 90-91 31.013911085580844 33.0 33.0 37.0 15.0 37.0 92-93 30.859329401602388 33.0 33.0 37.0 15.0 37.0 94-95 30.772231733072957 33.0 33.0 37.0 15.0 37.0 96-97 30.63188588960366 33.0 33.0 37.0 15.0 37.0 98-99 30.330330898094992 33.0 27.0 37.0 15.0 37.0 100-101 28.755238603956574 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16379819338258272 2306 2 0.15786275356027346 2306 3 0.17093102598490617 2306 4 0.19059049801263228 2306 5 0.22464786753484134 2306 6 0.21869377104324883 2306 7 0.30317780296067554 2306 8 0.284946409412818 2306 9 0.23428465078296057 2306 10-11 0.31974358602457187 2306 12-13 0.26570491353847814 2306 14-15 0.5899024736390217 2306 16-17 0.5700667093280103 2306 18-19 0.570946685872741 2306 20-21 0.6473991780111987 2306 22-23 0.6174476724148832 2306 24-25 0.5719212239305236 2306 26-27 0.6630128045239232 2306 28-29 0.6623809093759192 2306 30-31 0.6972778564045043 2306 32-33 0.644577722063076 2306 34-35 0.7246408678690912 2306 36-37 0.7082561755525418 2306 38-39 0.8336584997688732 2306 40-41 0.7979904807172744 2306 42-43 0.8283388789109836 2306 44-45 0.8511638342949084 2306 46-47 0.8200941273180504 2306 48-49 1.0416826291716603 2306 50-51 0.9571509614942144 2306 52-53 0.9134983893475024 2306 54-55 0.941699472045542 2306 56-57 0.950706396597873 2306 58-59 0.9425081035102565 2306 60-61 0.8635885625904649 2306 62-63 0.8590098775122854 2306 64-65 0.8719674528555785 2306 66-67 0.7802247283810502 2306 68-69 0.9607588917219942 2306 70-71 0.9170893614274505 2306 72-73 0.503115891448175 2306 74-75 0.4540146750396161 2306 76-77 0.2888093114444743 2306 78-79 0.7548899424035049 2306 80-81 0.8037506536084038 2306 82-83 0.8058396384449757 2306 84-85 0.7976161960997068 2306 86-87 0.7267089676182792 2306 88-89 0.7244674905083031 2306 90-91 0.8943189614705922 2306 92-93 0.7907378431811125 2306 94-95 0.8244275813320989 2306 96-97 0.8551533914653824 2306 98-99 0.7773248584572165 2306 100-101 0.6589580829469632 2307 1 0.13761796342416233 2307 2 0.08329098942629543 2307 3 0.12333360350820755 2307 4 0.1737067927022906 2307 5 0.21493610410591657 2307 6 0.22386291869050723 2307 7 0.24024964594920561 2307 8 0.26715815892624306 2307 9 0.2657517350385845 2307 10-11 0.33242521588316976 2307 12-13 0.248017559767284 2307 14-15 0.5503390124252547 2307 16-17 0.5107974034150331 2307 18-19 0.4751319142819028 2307 20-21 0.5666778351460522 2307 22-23 0.6278948426498019 2307 24-25 0.594421043028909 2307 26-27 0.5769885395874823 2307 28-29 0.5838549884939397 2307 30-31 0.656061566724425 2307 32-33 0.6906405793437571 2307 34-35 0.6468580351539188 2307 36-37 0.7176098094484971 2307 38-39 0.6870482620363205 2307 40-41 0.7475262036836128 2307 42-43 0.7184334858164192 2307 44-45 0.7637675373045454 2307 46-47 0.7232214430906296 2307 48-49 0.7501879736547181 2307 50-51 0.6439660355154402 2307 52-53 0.6267184195163296 2307 54-55 0.7120065594924512 2307 56-57 0.6791102877451962 2307 58-59 0.6191850058066919 2307 60-61 0.6474914360502524 2307 62-63 0.5400329086069533 2307 64-65 0.5527113573988984 2307 66-67 0.5753229440417513 2307 68-69 0.7048000667846566 2307 70-71 0.5530773903791371 2307 72-73 0.37338401547060585 2307 74-75 0.34799297055677414 2307 76-77 0.256095249173498 2307 78-79 0.5692758324487208 2307 80-81 0.5623657079623854 2307 82-83 0.5647202007057466 2307 84-85 0.5742880517049649 2307 86-87 0.5288296540424966 2307 88-89 0.5269648604771504 2307 90-91 0.6126278375242222 2307 92-93 0.6106965790591872 2307 94-95 0.637964180047792 2307 96-97 0.6819275353369001 2307 98-99 0.5436114029024246 2307 100-101 0.5211549892510625 2201 1 0.07809566771021537 2201 2 0.10559267129270467 2201 3 0.08462294351698318 2201 4 0.15282727606243185 2201 5 0.18541370637054655 2201 6 0.16287098111641285 2201 7 0.23731242932952767 2201 8 0.2397766106265351 2201 9 0.25349656226931216 2201 10-11 0.25194565387378987 2201 12-13 0.23728754051190748 2201 14-15 0.48951547687101993 2201 16-17 0.5127646497389478 2201 18-19 0.49679339508210063 2201 20-21 0.4162507888281297 2201 22-23 0.5021032681385904 2201 24-25 0.49867577623923864 2201 26-27 0.5508516859240089 2201 28-29 0.5387245807836791 2201 30-31 0.5984820455302824 2201 32-33 0.705180431743436 2201 34-35 0.6457809090292415 2201 36-37 0.663884764103976 2201 38-39 0.6654365583526598 2201 40-41 0.7256328408703112 2201 42-43 0.7148029903210613 2201 44-45 0.6963598195110023 2201 46-47 0.7208829188231007 2201 48-49 0.7899422860767018 2201 50-51 0.632578178157921 2201 52-53 0.6698248023162918 2201 54-55 0.727523650380391 2201 56-57 0.7301517363799874 2201 58-59 0.5894233245043949 2201 60-61 0.7322328260927335 2201 62-63 0.7696184395960941 2201 64-65 0.6511385205413447 2201 66-67 0.7772619711081674 2201 68-69 0.8221803754908024 2201 70-71 0.7791975271998552 2201 72-73 0.5146252667172888 2201 74-75 0.5386008855117268 2201 76-77 0.33584242409708764 2201 78-79 0.7065327805906954 2201 80-81 0.746527469654886 2201 82-83 0.8255063150706867 2201 84-85 0.761094035174434 2201 86-87 0.8370290220298102 2201 88-89 0.8721441917914348 2201 90-91 0.8566245272292825 2201 92-93 0.8357155847157749 2201 94-95 0.8985326820619797 2201 96-97 0.8600219232854123 2201 98-99 0.9268134497854668 2201 100-101 0.7879374624515982 1101 1 0.12869279433771652 1101 2 0.10228247508305088 1101 3 0.08813724997950345 1101 4 0.1429924942868368 1101 5 0.16314763824215817 1101 6 0.08223354618523615 1101 7 0.09997677626697055 1101 8 0.16216889387047217 1101 9 0.157120421785919 1101 10-11 0.17208994732601468 1101 12-13 0.20141342794024553 1101 14-15 0.40407242571433244 1101 16-17 0.44361154066770325 1101 18-19 0.46998483244871636 1101 20-21 0.43192909440756466 1101 22-23 0.4515033645008444 1101 24-25 0.4064186361987794 1101 26-27 0.4777670246012349 1101 28-29 0.46536430119428474 1101 30-31 0.47233427248421833 1101 32-33 0.5261958733089003 1101 34-35 0.49270793891959386 1101 36-37 0.5482125679470826 1101 38-39 0.5467290900001629 1101 40-41 0.6235270340030468 1101 42-43 0.6585400685452711 1101 44-45 0.7134766163962993 1101 46-47 0.6158147362791127 1101 48-49 0.632509409885536 1101 50-51 0.602928734045939 1101 52-53 0.5954897921316089 1101 54-55 0.6221749898018629 1101 56-57 0.7068352641271787 1101 58-59 0.6336215252794588 1101 60-61 0.5822423373528167 1101 62-63 0.5741912035127328 1101 64-65 0.5733544636348498 1101 66-67 0.5721922612085351 1101 68-69 0.6934645622225162 1101 70-71 0.6211995294242385 1101 72-73 0.5477987368276303 1101 74-75 0.35938583522701606 1101 76-77 0.3868019505612992 1101 78-79 0.817504886911312 1101 80-81 0.727615335647485 1101 82-83 0.6786698157142617 1101 84-85 0.6896714144820955 1101 86-87 0.6129347092079414 1101 88-89 0.7011622826246651 1101 90-91 0.6841055428930112 1101 92-93 0.6774343318953768 1101 94-95 0.7713421416696704 1101 96-97 0.8406994860146035 1101 98-99 0.7811939751680335 1101 100-101 0.7034809441837346 2304 1 0.08511628772798474 2304 2 0.14261334380088186 2304 3 0.09288072191669983 2304 4 0.1222441186632679 2304 5 0.1423368528666984 2304 6 0.1430450681280817 2304 7 0.048391918659064004 2304 8 0.09165590831419479 2304 9 0.09321619892268274 2304 10-11 0.08738530395777389 2304 12-13 0.17325389428002325 2304 14-15 0.24603624117972345 2304 16-17 0.29950924292477765 2304 18-19 0.38207129685963537 2304 20-21 0.3547113884081057 2304 22-23 0.40010758308030603 2304 24-25 0.33065763555654115 2304 26-27 0.3305813984271566 2304 28-29 0.3679938688286768 2304 30-31 0.3746282498714919 2304 32-33 0.3875860472242536 2304 34-35 0.42060132696473573 2304 36-37 0.36799735243854315 2304 38-39 0.4840920276823226 2304 40-41 0.42009486703556576 2304 42-43 0.5067630528550069 2304 44-45 0.41517840132071626 2304 46-47 0.4410424285004524 2304 48-49 0.44160655193240217 2304 50-51 0.3825535047852071 2304 52-53 0.4775921473223761 2304 54-55 0.4848729843262305 2304 56-57 0.594180975725429 2304 58-59 0.5002314038500444 2304 60-61 0.48701881147859893 2304 62-63 0.40734030311147507 2304 64-65 0.44130367091798206 2304 66-67 0.3568109147446066 2304 68-69 0.5758713303506084 2304 70-71 0.4943786684023266 2304 72-73 0.31251576401077585 2304 74-75 0.1571279033307178 2304 76-77 0.0930654638300723 2304 78-79 0.4379485684228861 2304 80-81 0.3032385606978707 2304 82-83 0.3335680908509637 2304 84-85 0.260985968397641 2304 86-87 0.384283130889024 2304 88-89 0.5102359107499836 2304 90-91 0.479627130464781 2304 92-93 0.379271687120049 2304 94-95 0.4056129738601584 2304 96-97 0.35502489476126087 2304 98-99 0.4230079245304559 2304 100-101 0.4202545739244279 2202 1 0.0827590392999511 2202 2 0.03531081274746839 2202 3 0.07317013952836504 2202 4 0.09330391985648845 2202 5 0.11423065190023607 2202 6 0.1522939872521647 2202 7 0.1862526990790485 2202 8 0.1461220453710652 2202 9 0.1088888871263265 2202 10-11 0.1548062528263614 2202 12-13 0.18131756175981906 2202 14-15 0.15067913658959498 2202 16-17 0.14207103350624806 2202 18-19 0.10492357854528223 2202 20-21 0.17619313767472988 2202 22-23 0.26340693054805087 2202 24-25 0.23011621734291055 2202 26-27 0.2275977847610804 2202 28-29 0.2119522076428666 2202 30-31 0.2639593874338644 2202 32-33 0.3264661717776107 2202 34-35 0.343297670004155 2202 36-37 0.3649282474420019 2202 38-39 0.31746006052696174 2202 40-41 0.27196129251417034 2202 42-43 0.3456477536111606 2202 44-45 0.3027479024007107 2202 46-47 0.3115457674653399 2202 48-49 0.4188996150842925 2202 50-51 0.37472063819431867 2202 52-53 0.3016754779593711 2202 54-55 0.3769482812073903 2202 56-57 0.4253602934256904 2202 58-59 0.3916419165571128 2202 60-61 0.39881000781191034 2202 62-63 0.360346656675155 2202 64-65 0.24272350839424206 2202 66-67 0.3252519234082456 2202 68-69 0.19682084908829722 2202 70-71 0.21294693380676932 2202 72-73 0.14261793529804834 2202 74-75 0.2854359108086726 2202 76-77 0.05967347143906565 2202 78-79 0.3118089654094831 2202 80-81 0.2679981452472049 2202 82-83 0.35316033707002603 2202 84-85 0.3929339218522152 2202 86-87 0.4526384970260722 2202 88-89 0.4998906181053435 2202 90-91 0.5190390722776534 2202 92-93 0.4758363195839337 2202 94-95 0.48678324499210746 2202 96-97 0.4578759346227095 2202 98-99 0.44645698237194864 2202 100-101 0.3897624476643706 1102 1 0.07886578073659933 1102 2 0.05140991828834274 1102 3 0.06850198099304805 1102 4 0.11873934246801099 1102 5 0.1457579634690589 1102 6 0.13744399067286395 1102 7 0.10988353441514676 1102 8 0.09108649211069064 1102 9 0.11173993419301809 1102 10-11 0.08778457769621184 1102 12-13 0.08804197764690258 1102 14-15 0.12642233986994 1102 16-17 0.19239484183201938 1102 18-19 0.17792900422440283 1102 20-21 0.14406071998681114 1102 22-23 0.18831515083261507 1102 24-25 0.20684356289809358 1102 26-27 0.2362022365324563 1102 28-29 0.17643205086424985 1102 30-31 0.11364893089115213 1102 32-33 0.12207657998906996 1102 34-35 0.16971000727240693 1102 36-37 0.1702810434819284 1102 38-39 0.154307754199543 1102 40-41 0.2169327737676099 1102 42-43 0.19746668577748494 1102 44-45 0.15674201658163156 1102 46-47 0.07538541475757654 1102 48-49 0.1574486679904581 1102 50-51 0.1832064135798106 1102 52-53 0.17010593172137334 1102 54-55 0.12742911091588383 1102 56-57 0.15622062274024273 1102 58-59 0.12872558368502496 1102 60-61 -0.0684600546214682 1102 62-63 0.10986168901736448 1102 64-65 0.19094286605209732 1102 66-67 0.13807868347118557 1102 68-69 0.286154898562728 1102 70-71 0.2538382867065323 1102 72-73 0.10756178056742982 1102 74-75 0.032878106415608954 1102 76-77 -0.007643377407713814 1102 78-79 0.26204764232501887 1102 80-81 0.13855978082958842 1102 82-83 0.26362656257612116 1102 84-85 0.18372872260168194 1102 86-87 0.18246944248679853 1102 88-89 0.15192880641183137 1102 90-91 0.3360294369247825 1102 92-93 0.2458401320145569 1102 94-95 0.08033345100681188 1102 96-97 0.14107150183073358 1102 98-99 0.16558263920243377 1102 100-101 0.11700841619830271 2305 1 0.01936151431814892 2305 2 -0.04255840955296719 2305 3 0.017494262232482072 2305 4 0.056903585362050535 2305 5 0.10818749249197879 2305 6 -0.006275026290047947 2305 7 0.03507144039332388 2305 8 0.04661295807162702 2305 9 -0.025336030302874235 2305 10-11 0.025317499901042595 2305 12-13 0.06032653174556657 2305 14-15 0.06907242023005011 2305 16-17 0.1201032917648277 2305 18-19 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72-73 0.35052051153638786 1213 74-75 0.3506611994143469 1213 76-77 -2.012332991688222 1213 78-79 -1.8644502095516096 1213 80-81 -1.3308086237648737 1213 82-83 -0.9455791692401334 1213 84-85 -0.6229826971141499 1213 86-87 -0.440513939879164 1213 88-89 -0.46424770644299684 1213 90-91 -1.6580256877031623 1213 92-93 -0.8681171659141214 1213 94-95 -0.5254615960656572 1213 96-97 -0.4376268687685503 1213 98-99 -2.1719728170998955 1213 100-101 -0.9135558958676313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 22.0 9 70.0 10 73.0 11 64.0 12 76.0 13 203.0 14 621.0 15 1549.0 16 3136.0 17 5188.0 18 7330.0 19 9521.0 20 12620.0 21 16849.0 22 21595.0 23 28665.0 24 36604.0 25 47772.0 26 63849.0 27 92653.0 28 150817.0 29 204770.0 30 151856.0 31 128924.0 32 166231.0 33 233963.0 34 353976.0 35 587425.0 36 1040912.0 37 1396114.0 38 350578.0 39 598.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.32228244094204 24.18393319639189 20.30577974363434 15.188004619031728 2 29.435875797296102 28.475395639339524 28.539666195896736 13.54906236746764 3 34.011590109986585 18.684384282983412 30.912871224019867 16.39115438301014 4 28.63933746084269 18.56153314044859 28.902465204689452 23.89666419401927 5 37.10273755388043 17.290030082708874 28.853455014560375 16.753777348850324 6 33.0560826930454 17.777331128414865 32.30099326910707 16.865592909432674 7 30.760978417581263 25.380604564243797 25.39624598162212 18.462171036552817 8 28.097284141597058 30.922065464806224 23.003558813489004 17.977091580107714 9 34.125818779320326 22.506259499717086 24.349561434208482 19.018360286754106 10-11 26.52204775159496 29.424524565829934 27.755825976718285 16.297601705856817 12-13 22.660415443866277 28.21002943323715 27.4094528123855 21.720102310511074 14-15 22.20481245401056 28.482319966535186 27.614622028533663 21.69824555092059 16-17 25.700021858880785 24.78567347837359 28.00148046015486 21.512824202590767 18-19 21.163741232250654 24.731265733069385 31.936671799007748 22.168321235672213 20-21 24.894048949033614 25.034332911145405 31.492322214762137 18.57929592505884 22-23 21.078980163945516 32.69096313200613 27.381562210022786 18.848494494025562 24-25 20.915165644565214 24.726089454048058 31.549012576872677 22.80973232451405 26-27 20.83411377488794 24.984133737246868 27.653801079492418 26.527951408372775 28-29 24.449676281315583 29.309591747275366 27.14009782924499 19.10063414216406 30-31 20.401227773057112 26.02735762106555 30.841785890112007 22.729628715765337 32-33 20.21995352152826 29.93345945529546 27.126939486875486 22.719647536300798 34-35 24.201750039983374 29.98451890714981 27.280899913307444 18.532831139559374 36-37 20.162090053114344 30.003053986743115 31.417771309182722 18.41708465095982 38-39 20.30454302123288 25.998853481416194 31.1651964351562 22.531407062194724 40-41 24.104334993312797 25.891710657377093 30.87630238131991 19.127651967990204 42-43 20.443408578312837 29.55858083993727 30.777072634100232 19.220937947649656 44-45 20.16375110309396 25.797172657875024 31.01155781453905 23.027518424491966 46-47 23.991306007270545 25.80130636704354 27.27418573984971 22.93320188583621 48-49 20.217669078477208 29.85107778700008 30.7113912343836 19.219861900139115 50-51 24.006657544350062 26.111513476795384 30.271452141665222 19.61037683718933 52-53 20.207378191561748 25.893580586983013 26.73055655925281 27.16848466220243 54-55 24.148022022258296 25.948381824352474 30.568570028675246 19.335026124713988 56-57 20.468668499847233 25.909773296669727 30.358389245340668 23.263168958142376 58-59 20.34947179905318 26.147904869562854 30.269525678571092 23.23309765281288 60-61 23.96682498882268 26.169693285326662 30.202278951313616 19.661202774537042 62-63 20.125756834078153 26.17752584437269 30.339892081333673 23.35682524021548 64-65 24.23269987122571 26.206786036685862 30.06527295291061 19.49524113917782 66-67 20.629454091679445 33.51240073102916 26.256479601131648 19.60166557615974 68-69 20.76289128378221 33.53159558295321 26.07086160028273 19.63465153298185 70-71 20.611520961262435 33.809320630385464 25.983210883558083 19.595947524794013 72-73 20.326616738283963 33.83656783016501 25.996483121545488 19.84033231000554 74-75 20.649643123241518 33.201522932340055 25.866712224638878 20.28212171977955 76-77 20.635975697231842 32.47987376282671 26.118072946834346 20.766077593107102 78-79 20.598836212784178 31.83560236934429 26.575259905405744 20.990301512465788 80-81 20.51055896552786 31.45011944000309 26.757102488663342 21.28221910580571 82-83 20.146262893904655 31.402657789640926 26.897235496789012 21.553843819665406 84-85 20.294913337384706 31.155935612895252 26.894378446697466 21.654772603022572 86-87 20.19395355773015 30.997924545367873 27.362676529761625 21.445445367140344 88-89 20.29346518953612 31.05165856450901 27.420932335167848 21.23394391078702 90-91 20.538748260321004 30.618252556067947 27.126055675790422 21.716943507820623 92-93 20.30435095120674 30.85499811993219 26.97164349220273 21.86900743665834 94-95 20.273503534650292 30.87331791062568 26.867195114593155 21.985983440130873 96-97 20.50365924425883 31.084194008888428 26.674608605888473 21.737538140964272 98-99 20.666187332351978 30.702888484749387 26.72756924692224 21.903354935976402 100-101 20.70011715621262 30.81642557285144 26.330342970143207 22.153114300792733 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2084.0 1 1284.0 2 530.5 3 737.5 4 780.5 5 568.5 6 509.0 7 521.5 8 502.0 9 485.0 10 482.0 11 472.0 12 447.5 13 452.0 14 466.5 15 496.5 16 530.0 17 601.0 18 657.5 19 714.0 20 826.5 21 1062.5 22 1515.0 23 2188.5 24 3154.5 25 4741.5 26 7751.5 27 12214.5 28 16979.0 29 21868.0 30 27367.5 31 32470.5 32 43035.5 33 63572.0 34 94128.0 35 129174.5 36 174138.5 37 232770.0 38 280019.5 39 297327.0 40 305897.5 41 309511.5 42 306019.5 43 306087.5 44 294150.5 45 272704.5 46 251333.0 47 221640.0 48 188892.5 49 165416.0 50 147118.5 51 133089.5 52 122350.5 53 107268.0 54 89086.5 55 73883.0 56 61485.0 57 52310.0 58 45811.0 59 38999.5 60 32125.5 61 26495.0 62 21516.0 63 17261.5 64 14199.0 65 11382.0 66 8722.0 67 6962.5 68 6103.5 69 5045.0 70 3557.0 71 2490.0 72 1829.0 73 1364.0 74 1021.5 75 816.0 76 642.5 77 484.5 78 326.0 79 199.5 80 132.0 81 86.5 82 65.5 83 47.5 84 30.5 85 23.0 86 18.0 87 13.0 88 7.5 89 5.5 90 4.0 91 2.0 92 2.0 93 3.0 94 3.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1719382805311669 2 0.006041497462375548 3 0.001818314770229534 4 0.0 5 0.001173106303373893 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2484537481332948E-4 12-13 0.0 14-15 9.775885861449107E-6 16-17 0.0 18-19 1.9551771722898214E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.3462126067477854E-4 40-41 8.798297275304196E-5 42-43 0.001280641047849833 44-45 6.745361244399884E-4 46-47 0.005670013799640482 48-49 0.004125423833531523 50-51 0.008368158297400436 52-53 0.00394945788802544 54-55 0.019190063946024596 56-57 0.011017423365853145 58-59 0.019444236978422274 60-61 0.0092968674542381 62-63 0.006090376891682793 64-65 0.012503358016793408 66-67 0.03051053977358266 68-69 0.03161521487592641 70-71 0.036161001801500246 72-73 0.0306767298332273 74-75 0.03375613387958377 76-77 0.033443305532017395 78-79 0.01741085271924086 80-81 0.0026101615250069114 82-83 0.0 84-85 0.006061049234098446 86-87 0.02519245786495435 88-89 0.02696189320587664 90-91 0.02452769762637581 92-93 0.03477282600917447 94-95 0.03133171418594439 96-97 0.03294473535308349 98-99 0.038712508011338465 100-101 0.02673704783106331 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5114626.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.813857832462 #Duplication Level Percentage of deduplicated Percentage of total 1 90.68580221378603 47.894670655416625 2 4.939279003324275 5.217247581528657 3 1.3376260161309808 2.119355707468324 4 0.5900482814420244 1.2465090420147038 5 0.4600277246314151 1.2147919423837266 6 0.2834535234090731 0.8982164452462336 7 0.21352160444048193 0.7893829762755159 8 0.19219279137790748 0.8120354208205467 9 0.1617479444618968 0.7688279649153219 >10 0.8918128015749522 9.438316762132953 >50 0.12790882808946605 4.728962976089268 >100 0.10044061247383657 11.513855106332521 >500 0.013823377418422985 4.833425678896858 >1k 0.002278330446289805 1.8324315561432176 >5k 0.0 0.0 >10k+ 3.694699290122079E-5 6.691970184335534 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 342947 6.705221457052773 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002268005519856193 0.0 0.0 2 0.00273724804120575 0.0 0.0 3 0.0032064905625553072 0.0 0.0 4 0.0034997671383987803 0.0 0.0 5 0.0041645273769773195 0.0 0.0 6 0.004985701789339044 0.0 0.0 7 0.006041497462375548 0.0 0.0 8 0.007175500222303645 0.0 0.0 9 0.008192192351894351 0.0 0.0 10-11 0.01181904600649197 0.0 0.0 12-13 0.015406796117643793 0.0 0.0 14-15 0.02027518727664545 0.0 0.0 16-17 0.026003856391454624 0.0 0.0 18-19 0.03271988997827016 0.0 0.0 20-21 0.038595197381001076 0.0 0.0 22-23 0.043835072202737795 0.0 0.0 24-25 0.05011119092578812 0.0 0.0 26-27 0.05636775787711555 1.9551771722898214E-5 0.0 28-29 0.06353348221355774 1.9551771722898214E-5 9.775885861449107E-6 30-31 0.0907788761094164 1.9551771722898214E-5 1.9551771722898214E-5 32-33 0.09937187978163017 1.9551771722898214E-5 1.9551771722898214E-5 34-35 0.10679177715047004 1.9551771722898214E-5 1.9551771722898214E-5 36-37 0.11562917796922004 1.9551771722898214E-5 1.9551771722898214E-5 38-39 0.12545394325997639 1.9551771722898214E-5 1.9551771722898214E-5 40-41 0.1360118999903414 1.9551771722898214E-5 1.9551771722898214E-5 42-43 0.14733237581789949 1.9551771722898214E-5 1.9551771722898214E-5 44-45 0.1597379749760784 1.9551771722898214E-5 1.9551771722898214E-5 46-47 0.1725541613404382 1.9551771722898214E-5 1.9551771722898214E-5 48-49 0.18690516178504546 1.9551771722898214E-5 1.9551771722898214E-5 50-51 0.2013245934306829 1.9551771722898214E-5 1.9551771722898214E-5 52-53 0.21841284191649596 1.9551771722898214E-5 1.9551771722898214E-5 54-55 0.23541310742955596 1.9551771722898214E-5 1.9551771722898214E-5 56-57 0.2533811856428994 1.9551771722898214E-5 1.9551771722898214E-5 58-59 0.27337287222956286 3.910354344579643E-5 2.932765758434732E-5 60-61 0.2957498749664198 3.910354344579643E-5 3.910354344579643E-5 62-63 0.3195346052673255 3.910354344579643E-5 3.910354344579643E-5 64-65 0.34522563331121375 3.910354344579643E-5 3.910354344579643E-5 66-67 0.37332152927701845 3.910354344579643E-5 3.910354344579643E-5 68-69 0.4043013115719507 3.910354344579643E-5 3.910354344579643E-5 70-71 0.43696254623505215 3.910354344579643E-5 4.887942930724553E-5 72-73 0.47321153100930546 3.910354344579643E-5 5.865531516869464E-5 74-75 0.5112983823255113 3.910354344579643E-5 7.820708689159286E-5 76-77 0.5539016929097064 3.910354344579643E-5 7.820708689159286E-5 78-79 0.6006597549850174 3.910354344579643E-5 8.798297275304196E-5 80-81 0.6538503499571621 3.910354344579643E-5 9.775885861449106E-5 82-83 0.7133561671958029 3.910354344579643E-5 9.775885861449106E-5 84-85 0.7773393401589872 3.910354344579643E-5 9.775885861449106E-5 86-87 0.8470609581228422 3.910354344579643E-5 9.775885861449106E-5 88-89 0.9250138719820373 3.910354344579643E-5 9.775885861449106E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 45905 0.0 81.006424 3 GATCGGA 46120 0.0 80.80661 1 ATCGGAA 46185 0.0 80.50661 2 CGGAAGA 46270 0.0 80.19292 4 GAAGAGC 47590 0.0 77.95863 6 GGAAGAG 52055 0.0 71.4086 5 GAGCACA 53290 0.0 69.26355 9 AGAGCAC 53495 0.0 69.07802 8 AAGAGCA 55340 0.0 66.80075 7 ATGCCGT 40360 0.0 45.475773 48-49 GCCGTCT 40550 0.0 45.463383 50-51 TCGTATG 40760 0.0 45.23594 44-45 CGTATCT 40250 0.0 45.1264 38-39 GTATGCC 40770 0.0 45.104065 46-47 TGCCGTC 40650 0.0 45.036034 50-51 TATGCCG 40530 0.0 45.02719 48-49 TATCTCG 40410 0.0 45.019123 40-41 TCTCGTA 40640 0.0 44.965206 42-43 CGTATGC 40630 0.0 44.943836 46-47 AATTCGT 40050 0.0 44.847775 34-35 >>END_MODULE