FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34211-2.34100000035863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34211-2.34100000035863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3402601
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT809852.3800909950946347TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG953.783498E-1045.079521
TCGGAAG229150.041.552063
CGGAAGA228150.041.4635854
GAGCACA230050.040.6882259
ATCGGAA235150.040.4936182
AGAGCAC233800.040.4615828
GATCGGA238150.040.001281
GAAGAGC242100.039.2313466
CGTCTTC123700.037.8258852-53
GCCGTCT124600.037.55210550-51
AAGAGCA255250.037.0613827
TGCCGTC125650.036.89805250-51
CCGTCTT126350.036.7693652-53
ATGCCGT126950.036.72357648-49
TATGCCG128400.036.01291748-49
CTGCTTG131700.035.67245558-59
TGCTTGA128600.035.3149560-61
CGTATGC131550.035.29189346-47
TCGTATG133950.035.01362644-45
GCTTGAA129300.034.81146660-61
Produced by FastQC (version 0.11.2)