##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34211-2.34100000035863.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3402601 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.685916450386046 33.0 33.0 33.0 33.0 33.0 2 32.7069609395871 33.0 33.0 33.0 33.0 33.0 3 32.731946531491644 33.0 33.0 33.0 33.0 33.0 4 36.661445758700474 37.0 37.0 37.0 37.0 37.0 5 36.630563501274466 37.0 37.0 37.0 37.0 37.0 6 36.51195835186083 37.0 37.0 37.0 37.0 37.0 7 36.430699044642616 37.0 37.0 37.0 37.0 37.0 8 36.396206020041724 37.0 37.0 37.0 37.0 37.0 9 36.38711444568435 37.0 37.0 37.0 37.0 37.0 10-11 36.34524471132525 37.0 37.0 37.0 37.0 37.0 12-13 36.31850546096942 37.0 37.0 37.0 37.0 37.0 14-15 38.147559176053846 40.0 37.0 40.0 37.0 40.0 16-17 38.13097700847087 40.0 37.0 40.0 37.0 40.0 18-19 38.115980980432326 40.0 37.0 40.0 37.0 40.0 20-21 38.04790394171988 40.0 37.0 40.0 33.0 40.0 22-23 37.92752999837477 40.0 37.0 40.0 33.0 40.0 24-25 37.910481128995144 40.0 37.0 40.0 33.0 40.0 26-27 37.84628729610084 40.0 37.0 40.0 33.0 40.0 28-29 37.66192186506734 40.0 37.0 40.0 33.0 40.0 30-31 37.59377914130984 40.0 37.0 40.0 33.0 40.0 32-33 37.43501603626167 40.0 37.0 40.0 33.0 40.0 34-35 37.33560032457523 40.0 37.0 40.0 33.0 40.0 36-37 37.30543707593103 40.0 37.0 40.0 33.0 40.0 38-39 37.15308729998022 40.0 37.0 40.0 33.0 40.0 40-41 37.034016918234016 40.0 37.0 40.0 33.0 40.0 42-43 36.895208988653096 38.5 37.0 40.0 33.0 40.0 44-45 36.76912088722715 37.0 37.0 40.0 33.0 40.0 46-47 36.72162104813347 37.0 37.0 40.0 33.0 40.0 48-49 36.504188413510725 37.0 37.0 40.0 33.0 40.0 50-51 36.38012376414396 37.0 37.0 40.0 33.0 40.0 52-53 36.27443388160998 37.0 37.0 40.0 33.0 40.0 54-55 36.024285098370335 37.0 37.0 40.0 30.0 40.0 56-57 35.859694686506 37.0 37.0 40.0 27.0 40.0 58-59 35.683187361668324 37.0 37.0 40.0 27.0 40.0 60-61 35.43523616198314 37.0 33.0 40.0 27.0 40.0 62-63 35.24495158262752 37.0 33.0 40.0 27.0 40.0 64-65 34.974279088262186 37.0 33.0 40.0 27.0 40.0 66-67 34.5657964304366 37.0 33.0 38.5 27.0 40.0 68-69 34.34749872230097 37.0 33.0 37.0 27.0 40.0 70-71 34.054686841037196 37.0 33.0 37.0 27.0 40.0 72-73 33.58825748302549 37.0 33.0 37.0 27.0 40.0 74-75 33.069549441735894 37.0 33.0 37.0 22.0 40.0 76-77 30.407364101756272 33.0 30.0 35.0 22.0 37.0 78-79 32.64425155932182 35.0 33.0 37.0 22.0 37.0 80-81 32.92996166755961 37.0 33.0 37.0 22.0 37.0 82-83 32.79414924053687 37.0 33.0 37.0 22.0 37.0 84-85 32.613545343694426 37.0 33.0 37.0 22.0 37.0 86-87 32.40583292016901 37.0 33.0 37.0 22.0 37.0 88-89 32.20809874563606 35.0 33.0 37.0 22.0 37.0 90-91 32.01628298469318 33.0 33.0 37.0 22.0 37.0 92-93 31.833459462334844 33.0 33.0 37.0 22.0 37.0 94-95 31.71295003440015 33.0 33.0 37.0 22.0 37.0 96-97 31.56114836855688 33.0 33.0 37.0 15.0 37.0 98-99 31.246194455359294 33.0 33.0 37.0 15.0 37.0 100-101 29.549127711418414 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13733229913881217 2306 2 0.14079618058683252 2306 3 0.13067940513607113 2306 4 0.1767885207122717 2306 5 0.21277002846098725 2306 6 0.22788481890235346 2306 7 0.1943643503023722 2306 8 0.1922595006546146 2306 9 0.2446224475980756 2306 10-11 0.2072367986749768 2306 12-13 0.23269825468702976 2306 14-15 0.5174236847616527 2306 16-17 0.5368154481720495 2306 18-19 0.5069015315543055 2306 20-21 0.5746989240285245 2306 22-23 0.6288817150481236 2306 24-25 0.5877594789809422 2306 26-27 0.5922223484610072 2306 28-29 0.51994295595415 2306 30-31 0.6871020877977472 2306 32-33 0.6512560999874282 2306 34-35 0.7483445035745859 2306 36-37 0.5931349870336717 2306 38-39 0.5530787807477395 2306 40-41 0.6716206263737234 2306 42-43 0.75448850849763 2306 44-45 0.8226035281955291 2306 46-47 0.7130650506576188 2306 48-49 0.7205147416480813 2306 50-51 0.7364727149787598 2306 52-53 0.7311145666598904 2306 54-55 0.8217184878266011 2306 56-57 0.8824227539547351 2306 58-59 0.7860411385419468 2306 60-61 0.7499999573693401 2306 62-63 0.8065603722918482 2306 64-65 0.7677243937474927 2306 66-67 0.798792378147958 2306 68-69 0.7587788077799047 2306 70-71 0.6680151952867703 2306 72-73 0.4652870004409664 2306 74-75 0.6027247825249589 2306 76-77 0.27837883590971657 2306 78-79 0.5861813699604781 2306 80-81 0.5033958787920767 2306 82-83 0.6917290358733865 2306 84-85 0.5867255014554686 2306 86-87 0.4548072531029632 2306 88-89 0.49706592030372576 2306 90-91 0.6579615074702261 2306 92-93 0.7295375007184823 2306 94-95 0.8504995645655171 2306 96-97 0.8336463344012195 2306 98-99 0.6642878088974236 2306 100-101 0.5452839871011292 2307 1 0.141188807336583 2307 2 0.08591610725809318 2307 3 0.1232629259824023 2307 4 0.17547631075868253 2307 5 0.21775194121328667 2307 6 0.22390769276625377 2307 7 0.25054640065491895 2307 8 0.2443520803653314 2307 9 0.2476409441629741 2307 10-11 0.2550397124473065 2307 12-13 0.22470557449765494 2307 14-15 0.5471089786263619 2307 16-17 0.5140420629338252 2307 18-19 0.5057645877385397 2307 20-21 0.5312267167746825 2307 22-23 0.47093350455893557 2307 24-25 0.5191542164246385 2307 26-27 0.5568978393967043 2307 28-29 0.5410070064743451 2307 30-31 0.5604795969066885 2307 32-33 0.5934421150559714 2307 34-35 0.6936387166350713 2307 36-37 0.5718512551059476 2307 38-39 0.596870168322134 2307 40-41 0.5199329005552755 2307 42-43 0.5469639304154796 2307 44-45 0.5983260111397399 2307 46-47 0.6289916261205803 2307 48-49 0.5947486598913301 2307 50-51 0.5896866713886553 2307 52-53 0.6078971375855318 2307 54-55 0.7401350399171207 2307 56-57 0.6737746219543013 2307 58-59 0.5935476457055415 2307 60-61 0.584621224663266 2307 62-63 0.4293038207674229 2307 64-65 0.35385986249732326 2307 66-67 0.7309112586654365 2307 68-69 0.6342243484541683 2307 70-71 0.4838908238227049 2307 72-73 0.6204538135256144 2307 74-75 0.6709789685008403 2307 76-77 0.17156305150666284 2307 78-79 0.4095817943004718 2307 80-81 0.6221773328043128 2307 82-83 0.678176608981687 2307 84-85 0.6767296708300492 2307 86-87 0.6916974004244949 2307 88-89 0.7504332617994436 2307 90-91 0.8049983667010352 2307 92-93 0.6998876955251667 2307 94-95 0.790546897308829 2307 96-97 0.8641818323257944 2307 98-99 0.6663271011705838 2307 100-101 0.4514573830077424 2201 1 0.05439598678478319 2201 2 0.03636080311380141 2201 3 0.1015832843506459 2201 4 0.16871510176283522 2201 5 0.20760038309683893 2201 6 0.18424961208662438 2201 7 0.3077132295695506 2201 8 0.1839777114514547 2201 9 0.31265492282419416 2201 10-11 0.23229517844977465 2201 12-13 0.26181397465473566 2201 14-15 0.4176867470493022 2201 16-17 0.4893592260402926 2201 18-19 0.4336252435321555 2201 20-21 0.35501158668883903 2201 22-23 0.4432127815469187 2201 24-25 0.4321732272090202 2201 26-27 0.4234294455931149 2201 28-29 0.38173602118580163 2201 30-31 0.5433955193277953 2201 32-33 0.5597392656308529 2201 34-35 0.5602256722882686 2201 36-37 0.5747158818333062 2201 38-39 0.6117895754203104 2201 40-41 0.535418275338337 2201 42-43 0.611951598595482 2201 44-45 0.7411407648882502 2201 46-47 0.6755644910493857 2201 48-49 0.7054187293802343 2201 50-51 0.5648670199927821 2201 52-53 0.607397823119733 2201 54-55 0.7431697103101271 2201 56-57 0.7418390572972626 2201 58-59 0.737095584864008 2201 60-61 0.6325439147248204 2201 62-63 0.6788416820371239 2201 64-65 0.5680498845883832 2201 66-67 0.5796880649043388 2201 68-69 0.7174014267629332 2201 70-71 0.6856393989152565 2201 72-73 0.6723285327586765 2201 74-75 0.6300135492433228 2201 76-77 0.48162008678317036 2201 78-79 0.7257154788300113 2201 80-81 0.7549327125668697 2201 82-83 0.6922592453308809 2201 84-85 0.5785254098963364 2201 86-87 0.8627082198790532 2201 88-89 0.8597002030695791 2201 90-91 0.9097589072157675 2201 92-93 0.9219502033817655 2201 94-95 1.025234733193784 2201 96-97 0.951893668241933 2201 98-99 0.971547490429284 2201 100-101 0.7602494688149903 1101 1 0.14146347119273628 1101 2 0.1374507117102013 1101 3 0.1157767390193527 1101 4 0.143819704454188 1101 5 0.1970627265602829 1101 6 0.10515709393516914 1101 7 0.14555454475762986 1101 8 0.21633636703774783 1101 9 0.14497414974567135 1101 10-11 0.16906673170570485 1101 12-13 0.21501249538807343 1101 14-15 0.3374861623637031 1101 16-17 0.3748417818824308 1101 18-19 0.33364296913804736 1101 20-21 0.3898514812344587 1101 22-23 0.4407236741479039 1101 24-25 0.43377527611612265 1101 26-27 0.4165222525422436 1101 28-29 0.4458966618670175 1101 30-31 0.39038921333541765 1101 32-33 0.49056772586367003 1101 34-35 0.5027537241404261 1101 36-37 0.5613405719594553 1101 38-39 0.5321932177296915 1101 40-41 0.5511551547151612 1101 42-43 0.6045730773606408 1101 44-45 0.549796740276463 1101 46-47 0.5055493853745148 1101 48-49 0.5776268816025478 1101 50-51 0.6550279252610949 1101 52-53 0.6537053200134224 1101 54-55 0.622099844618333 1101 56-57 0.6359412726521327 1101 58-59 0.6613449054545129 1101 60-61 0.6018739473485795 1101 62-63 0.7728763671568615 1101 64-65 0.7299513319489535 1101 66-67 0.7404670058469378 1101 68-69 0.7359489819695426 1101 70-71 0.6924117908949796 1101 72-73 0.6957564228322468 1101 74-75 0.4958711789061283 1101 76-77 0.5159532019330335 1101 78-79 0.8612856683383612 1101 80-81 0.6489283148872858 1101 82-83 0.6353514037822308 1101 84-85 0.6256198372252442 1101 86-87 0.6279338668420493 1101 88-89 0.7293096950018381 1101 90-91 0.7038134796006617 1101 92-93 0.7885146956209788 1101 94-95 0.9073269426603048 1101 96-97 0.8286160567197776 1101 98-99 0.8327861730622566 1101 100-101 0.8123656899925074 2304 1 0.0802748007082954 2304 2 0.08262638111122556 2304 3 0.08484499470732487 2304 4 0.13150195178432256 2304 5 0.15353076556353784 2304 6 0.15812799627227037 2304 7 0.1283041191269234 2304 8 0.22422910809950025 2304 9 0.1537808360171482 2304 10-11 0.16733170616786452 2304 12-13 0.12063004685069956 2304 14-15 0.30437131946013096 2304 16-17 0.279161546624735 2304 18-19 0.3055203874943402 2304 20-21 0.24404897388264857 2304 22-23 0.33702455636426976 2304 24-25 0.35688315415357863 2304 26-27 0.33905901155787177 2304 28-29 0.3106939068755281 2304 30-31 0.4066142558984893 2304 32-33 0.43861872416115233 2304 34-35 0.4123915511021963 2304 36-37 0.4447502608621008 2304 38-39 0.5217358581878244 2304 40-41 0.40296447215827413 2304 42-43 0.4026681864768804 2304 44-45 0.3972441168435594 2304 46-47 0.3330021790597044 2304 48-49 0.4509247457102745 2304 50-51 0.3798100797263473 2304 52-53 0.4214502511386442 2304 54-55 0.39746279153057884 2304 56-57 0.523388262282225 2304 58-59 0.5202208534501693 2304 60-61 0.4099644132207345 2304 62-63 0.36911456557816535 2304 64-65 0.2723859395948338 2304 66-67 0.19316356984631966 2304 68-69 0.42406343793611256 2304 70-71 0.4501462747210212 2304 72-73 0.3543139216714408 2304 74-75 0.17923250068294294 2304 76-77 0.18522343873847902 2304 78-79 0.3324097203426888 2304 80-81 0.2762681229665276 2304 82-83 0.27664686888051904 2304 84-85 0.19972330418082862 2304 86-87 0.4109334025176423 2304 88-89 0.38191765042669346 2304 90-91 0.3703616145642812 2304 92-93 0.30892484884803295 2304 94-95 0.42543133826470125 2304 96-97 0.3255410683278832 2304 98-99 0.1971953202530905 2304 100-101 0.21924388222281266 2202 1 0.10853920805666917 2202 2 0.03121738298467136 2202 3 0.0775064045443088 2202 4 0.08852611083138129 2202 5 0.16587816371246333 2202 6 0.16266243130288416 2202 7 0.09616098236546833 2202 8 0.06890419128603043 2202 9 0.06928848358586492 2202 10-11 0.14572361959795188 2202 12-13 0.09933471368513125 2202 14-15 0.1292955428194631 2202 16-17 0.10891492301932715 2202 18-19 0.09262997002376494 2202 20-21 0.12456766638607775 2202 22-23 0.21091377147486412 2202 24-25 0.24525381657004175 2202 26-27 0.18845000224657582 2202 28-29 0.10193723098144858 2202 30-31 0.15895634129400804 2202 32-33 0.12752842133419762 2202 34-35 0.18706621486082753 2202 36-37 0.1799002762411206 2202 38-39 0.2605803343223769 2202 40-41 0.14995661487561307 2202 42-43 0.19153300486459557 2202 44-45 0.11094479911294286 2202 46-47 0.20391156865230187 2202 48-49 0.17419509495390884 2202 50-51 0.12900051397403445 2202 52-53 0.16325882424492733 2202 54-55 0.20086966455577482 2202 56-57 0.2278346174537731 2202 58-59 0.2537179733785919 2202 60-61 0.23665771355908305 2202 62-63 0.31958614234745397 2202 64-65 0.24517038161591387 2202 66-67 0.1998333822222378 2202 68-69 0.27473948580681906 2202 70-71 0.2325605716200485 2202 72-73 0.06016577973704074 2202 74-75 0.2228042787776232 2202 76-77 0.13249673795834127 2202 78-79 0.3168588017955827 2202 80-81 0.362245879306883 2202 82-83 0.32394351625350026 2202 84-85 0.3939632309207539 2202 86-87 0.3849518014215363 2202 88-89 0.43261809502026694 2202 90-91 0.3654612286906129 2202 92-93 0.2082534845888837 2202 94-95 0.34640364204234686 2202 96-97 0.2654385182062491 2202 98-99 0.2608451119372326 2202 100-101 0.2876520135317051 1102 1 0.11171955691560953 1102 2 0.09394937332891828 1102 3 0.07839415030052521 1102 4 0.07309044244026097 1102 5 0.14758762405260484 1102 6 0.14648074125611998 1102 7 0.09243112426219113 1102 8 0.16495360383775903 1102 9 0.03807328135570742 1102 10-11 0.08246432303483431 1102 12-13 0.061746304990357714 1102 14-15 0.04115172075188411 1102 16-17 0.07606238838448576 1102 18-19 0.059700068790334626 1102 20-21 0.1299995271146983 1102 22-23 0.10998500953807877 1102 24-25 0.11595721842378737 1102 26-27 0.16139264488943894 1102 28-29 0.050751877122763744 1102 30-31 0.05696790087704784 1102 32-33 0.00844415165862955 1102 34-35 0.0035064857202229405 1102 36-37 0.24492549512542183 1102 38-39 0.10566751153103837 1102 40-41 0.0996948443325607 1102 42-43 0.0900930104485056 1102 44-45 0.10856988845666393 1102 46-47 0.20810131082951955 1102 48-49 0.05471279695702691 1102 50-51 0.08765022478431916 1102 52-53 0.06860732668525316 1102 54-55 -0.024212786848366363 1102 56-57 0.03674762487420935 1102 58-59 0.14285426443011318 1102 60-61 -0.10411269514921173 1102 62-63 0.07147546345523637 1102 64-65 0.11092000704149285 1102 66-67 0.023215319541911583 1102 68-69 0.1332292222550322 1102 70-71 0.16000108439860838 1102 72-73 0.11907859952627575 1102 74-75 0.03068162451162948 1102 76-77 -0.13423471150769828 1102 78-79 0.07468339814457892 1102 80-81 7.091997837278541E-4 1102 82-83 -2.1214959584625603E-4 1102 84-85 -0.11169829039803147 1102 86-87 0.11526978877418514 1102 88-89 0.01185306257133334 1102 90-91 0.06943210532610777 1102 92-93 0.03125371750174466 1102 94-95 0.06897771446248413 1102 96-97 0.17182236961434683 1102 98-99 -0.044417655552933866 1102 100-101 -0.11563673612648984 2305 1 0.061847253231753996 2305 2 0.015052795554929332 2305 3 0.07859237764779436 2305 4 0.019984796189355336 2305 5 0.0407531109415018 2305 6 0.029246619883686265 2305 7 0.0062431077719651284 2305 8 -0.004202749828031926 2305 9 0.0036761536358085323 2305 10-11 0.015241506901880086 2305 12-13 -0.038147386091004876 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-0.6345463967174041 1213 66-67 -0.6226051348212494 1213 68-69 -0.10977882275777517 1213 70-71 -0.029200061507040687 1213 72-73 0.1585275049147512 1213 74-75 0.15706955647777932 1213 76-77 -2.304758814098964 1213 78-79 -1.9440267826946709 1213 80-81 -1.470524165084651 1213 82-83 -0.8904288476699378 1213 84-85 -0.6690931424584363 1213 86-87 -0.41583100214572255 1213 88-89 -0.5677365254175228 1213 90-91 -1.7399385088942374 1213 92-93 -0.9309176012424558 1213 94-95 -0.5613190019477088 1213 96-97 -0.4151261337933576 1213 98-99 -2.2314456890203296 1213 100-101 -0.6309807281112256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 35.0 9 137.0 10 198.0 11 163.0 12 149.0 13 218.0 14 493.0 15 1108.0 16 2145.0 17 3432.0 18 5020.0 19 6857.0 20 9275.0 21 11940.0 22 15132.0 23 19120.0 24 23972.0 25 29899.0 26 37228.0 27 46363.0 28 59247.0 29 78301.0 30 89210.0 31 91929.0 32 116680.0 33 160671.0 34 235348.0 35 367809.0 36 613795.0 37 944086.0 38 430935.0 39 1701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.22111083142336 29.065563224888084 15.95453749214298 17.758788451545566 2 30.96686038497362 27.28580107413583 27.333326867239034 14.414011673651517 3 35.026916585183635 22.552121953276792 24.334638531289336 18.086322930250233 4 29.90562219901775 23.56782355615601 26.46601820195785 20.06053604286838 5 34.572683760060805 21.35035305749021 26.232997370804707 17.843965811644285 6 31.86635753060673 21.949385190917184 28.108438221231346 18.07581905724474 7 32.270283821112145 24.516303851083332 22.958436795851174 20.254975531953352 8 29.124073025312107 28.660809774640043 22.710214920879647 19.504902279168203 9 31.07299386557519 23.08075498714072 24.968046503248544 20.87820464403555 10-11 25.845362142717544 29.999941221332545 27.749217876356134 16.40547875959378 12-13 24.50134470659357 29.499153735627537 27.258456104609387 18.7410454531695 14-15 23.915149603957325 29.612894608109297 27.438557656122132 19.033398131811243 16-17 25.060534573404286 27.976597902604507 27.495304327483595 19.467563196507612 18-19 22.970739585796217 28.01408804824428 29.2333907551004 19.781781610859106 20-21 24.205644446704152 28.15619580432734 29.1490686095725 18.48909113939601 22-23 22.692625435659366 31.477081797131078 27.31585337217029 18.514439395039265 24-25 22.27003695114414 28.435232341376494 29.114286394437666 20.180444313041697 26-27 22.23309462378927 28.292106538498047 27.611480158854945 21.86331867885773 28-29 23.53984495978224 29.98116734815513 27.50531137797232 18.97367631409031 30-31 21.90318523976217 28.399641920989264 29.16138271869079 20.535790120557774 32-33 21.861246734483412 29.88975198678893 27.583575035685936 20.665426243041722 34-35 21.656506302090666 29.86468292932377 29.272562372138257 19.206248396447307 36-37 21.706056631382875 28.247875669230687 29.209301942837257 20.83676575654918 38-39 23.020905934097133 29.90194111941212 27.669338595194233 19.407814351296516 40-41 21.471713889168495 31.30188040166373 27.72521580939268 19.5011898997751 42-43 21.4883102379098 29.795374057692026 29.08055722913697 19.6357584752612 44-45 21.323641675110007 28.286485338709642 29.21651422118347 21.17335876499688 46-47 22.86268095655867 28.167751928415342 27.66069882921051 21.308868285815475 48-49 21.277575636451274 29.815119060914753 29.060694321167624 19.846610981466352 50-51 22.661926961036304 28.237488467431497 29.10317691952053 19.99740765201167 52-53 21.285043007093098 28.270749239815167 27.572137469867407 22.872070283224325 54-55 22.474235137804534 28.267110925594956 29.328144106857305 19.930509829743205 56-57 21.128317543624508 28.188642343695992 29.223541362453176 21.45949875022632 58-59 20.972964551661597 28.451044960606836 29.14466746376497 21.431323023966595 60-61 22.397496970779134 28.294399854215303 29.140759388380282 20.167343786625285 62-63 20.937846906982124 28.354356306700968 29.078112467445667 21.629684318871238 64-65 22.323954798207964 28.336007120169914 29.113702858708244 20.226335222913875 66-67 20.939715264346365 31.01979367586632 27.600578678898767 20.439912380888543 68-69 20.847621113929467 31.219035792360327 27.507828880602368 20.425514213107835 70-71 20.790562338317923 31.124970636667605 27.58959601640642 20.49487100860805 72-73 20.76306614063042 31.116597188332346 27.515919260099835 20.604417410937398 74-75 20.570353981845585 31.05567464757118 27.744906813498964 20.629064557084277 76-77 20.744836839922744 30.77999999706006 27.644582961369622 20.83058020164757 78-79 20.65518412595159 30.750714541760736 27.689975809856865 20.904125522430814 80-81 20.67187574852631 30.483209645559654 27.738684192505612 21.10623041340842 82-83 20.72028133771782 30.521386433496023 27.592553461307983 21.165778767478173 84-85 20.652454725301013 30.353394974190255 27.74615954575554 21.247990754753193 86-87 20.829393530821 30.03827922654885 27.67160500976787 21.46072223286228 88-89 20.742958286811902 30.222056420721895 27.633377605861615 21.40160768660459 90-91 20.749660172712222 29.959420623871164 27.701788620371765 21.589130583044852 92-93 20.801549945463794 29.945625490605366 27.573609179722524 21.679215384208312 94-95 20.633030493809176 29.92819547341466 27.75079071117041 21.687983321605756 96-97 20.688386453410228 29.785966445154987 27.69237531001248 21.833271791422312 98-99 20.630556943112012 29.819055450872224 27.66298871919049 21.887398886825277 100-101 20.740655052842513 29.750200232856592 27.57139923704998 21.937745477250918 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 2.5 2 11.5 3 22.0 4 20.5 5 13.5 6 15.0 7 16.5 8 17.0 9 20.5 10 19.0 11 27.0 12 42.5 13 60.0 14 84.0 15 112.0 16 170.5 17 308.0 18 500.5 19 780.5 20 1305.0 21 2039.5 22 3210.5 23 4948.5 24 7228.5 25 10262.0 26 14112.0 27 18926.0 28 24607.5 29 31406.5 30 39042.5 31 47489.0 32 57634.0 33 69238.0 34 81492.0 35 95242.5 36 111420.5 37 127586.0 38 142337.5 39 154837.5 40 163646.0 41 169255.5 42 170710.0 43 169208.0 44 166280.5 45 162199.0 46 157222.5 47 151311.0 48 144230.5 49 134929.5 50 124235.0 51 112270.5 52 98587.0 53 83900.0 54 69661.5 55 56975.0 56 46471.0 57 37752.0 58 30229.0 59 24259.0 60 19457.0 61 15315.5 62 11728.5 63 8865.5 64 6713.0 65 5077.5 66 3818.0 67 2796.0 68 2060.0 69 1557.0 70 1224.0 71 948.5 72 723.0 73 576.0 74 447.0 75 310.5 76 235.0 77 192.0 78 140.5 79 100.5 80 85.5 81 65.5 82 50.0 83 44.0 84 36.0 85 27.5 86 25.5 87 18.0 88 7.0 89 4.0 90 3.0 91 3.0 92 2.0 93 0.5 94 0.0 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17504256302751925 2 0.006994649093443515 3 0.0015282426590716924 4 0.0 5 0.0011461819943037693 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.7633569143134914E-4 12-13 0.0 14-15 1.4694640952612427E-5 16-17 0.0 18-19 1.4694640952612427E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.347320476306213E-5 40-41 1.0286248666828699E-4 42-43 0.0014988533771664676 44-45 5.437017152466598E-4 46-47 0.005760299253424072 48-49 0.004143888748636705 50-51 0.00822899893346296 52-53 0.004085110184826255 54-55 0.02001410097745813 56-57 0.01131487353351157 58-59 0.019940627772695067 60-61 0.00950743269634024 62-63 0.006230527763907669 64-65 0.012784337628772813 66-67 0.031152638819538347 68-69 0.03212248512241077 70-71 0.03673660238153107 72-73 0.031182028101443573 74-75 0.03437076518816047 76-77 0.03442954375197092 78-79 0.0175013173745614 80-81 0.002409921116228438 82-83 0.0 84-85 0.0059954135086658705 86-87 0.026053598408981835 88-89 0.02771409283662704 90-91 0.0256568431032613 92-93 0.0350026347491228 94-95 0.03157878340716411 96-97 0.03306294214337796 98-99 0.03932285918919086 100-101 0.02752306250424308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3402601.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.81910765881395 #Duplication Level Percentage of deduplicated Percentage of total 1 97.69358890698719 90.67831746333556 2 1.655374395931028 3.0730074854313236 3 0.2937672322500995 0.8180163707056135 4 0.13498874910146264 0.5011814094230913 5 0.05274209681388985 0.24477371811600152 6 0.030803329905172157 0.1715482556830883 7 0.03587564462032106 0.23309617242400507 8 0.018195747846756056 0.13511304626565457 9 0.01766561642851453 0.14757360778238424 >10 0.06276916762579181 1.1133305355570244 >50 0.0033235864626905164 0.22642654017368505 >100 7.354542829549917E-4 0.12558953451313723 >500 3.884213829080502E-5 0.025200995383832272 >1k 9.956024644737138E-5 0.12625323877204245 >5k 0.0 0.0 >10k+ 3.1669359322597716E-5 2.3805716264335195 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 80985 2.3800909950946347 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003879385211489681 0.0 0.0 2 0.004672895822930752 0.0 0.0 3 0.006965259811538291 0.0 0.0 4 0.010315637948733924 0.0 0.0 5 0.013048841165919835 0.0 0.0 6 0.023158754141317187 0.0 0.0 7 0.03297477429766229 0.0 0.0 8 0.04179155886922974 0.0 0.0 9 0.04661140110168662 0.0 2.9389281905224857E-5 10-11 0.055780857056116774 0.0 2.9389281905224857E-5 12-13 0.06819782866107427 0.0 2.9389281905224857E-5 14-15 0.08035029672888475 0.0 4.4083922857837286E-5 16-17 0.09461879309387142 0.0 5.8778563810449715E-5 18-19 0.11179682836747534 0.0 8.816784571567457E-5 20-21 0.13305997382590554 0.0 1.3225176857351186E-4 22-23 0.15698284929675854 0.0 1.469464095261243E-4 24-25 0.178995421443772 0.0 1.7633569143134914E-4 26-27 0.2008757418222119 0.0 1.7633569143134914E-4 28-29 0.22446064055115483 0.0 2.05724973336574E-4 30-31 0.2546140437859155 0.0 2.2041961428918643E-4 32-33 0.2892199232293178 0.0 2.3511425524179886E-4 34-35 0.33134945884045763 0.0 2.3511425524179886E-4 36-37 0.3799005525478891 0.0 2.3511425524179886E-4 38-39 0.4358430506544846 0.0 2.3511425524179886E-4 40-41 0.49997046377168525 0.0 2.3511425524179886E-4 42-43 0.5684034066880013 0.0 2.3511425524179886E-4 44-45 0.6385262333138678 0.0 2.645035371470237E-4 46-47 0.7129105058159919 0.0 2.938928190522486E-4 48-49 0.7949653808953797 0.0 2.938928190522486E-4 50-51 0.878857086093844 0.0 3.2328210095747343E-4 52-53 0.9711688205581555 0.0 3.526713828626983E-4 54-55 1.066434177853942 0.0 3.526713828626983E-4 56-57 1.1654319739516916 2.9389281905224857E-5 3.526713828626983E-4 58-59 1.2714244191428852 2.9389281905224857E-5 3.8206066476792315E-4 60-61 1.3791802212483921 2.9389281905224857E-5 3.8206066476792315E-4 62-63 1.4897867836987058 2.9389281905224857E-5 4.11449946673148E-4 64-65 1.6029649083157267 2.9389281905224857E-5 4.4083922857837286E-4 66-67 1.7182296719480186 2.9389281905224857E-5 4.702285104835977E-4 68-69 1.838593475990867 2.9389281905224857E-5 4.996177923888226E-4 70-71 1.9648792203376182 2.9389281905224857E-5 5.290070742940474E-4 72-73 2.0980273620092396 2.9389281905224857E-5 5.730909971518848E-4 74-75 2.2374354207266736 2.9389281905224857E-5 5.877856381044971E-4 76-77 2.3811783985251282 2.9389281905224857E-5 5.877856381044971E-4 78-79 2.529373852532225 2.9389281905224857E-5 5.877856381044971E-4 80-81 2.6892368514556955 2.9389281905224857E-5 6.024802790571095E-4 82-83 2.852729426694461 2.9389281905224857E-5 6.318695609623345E-4 84-85 3.0233782920771493 2.9389281905224857E-5 6.465642019149469E-4 86-87 3.201844706446627 2.9389281905224857E-5 6.612588428675592E-4 88-89 3.3904945069962658 2.9389281905224857E-5 6.759534838201717E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGG 95 3.783498E-10 45.07952 1 TCGGAAG 22915 0.0 41.55206 3 CGGAAGA 22815 0.0 41.463585 4 GAGCACA 23005 0.0 40.688225 9 ATCGGAA 23515 0.0 40.493618 2 AGAGCAC 23380 0.0 40.461582 8 GATCGGA 23815 0.0 40.00128 1 GAAGAGC 24210 0.0 39.231346 6 CGTCTTC 12370 0.0 37.82588 52-53 GCCGTCT 12460 0.0 37.552105 50-51 AAGAGCA 25525 0.0 37.061382 7 TGCCGTC 12565 0.0 36.898052 50-51 CCGTCTT 12635 0.0 36.76936 52-53 ATGCCGT 12695 0.0 36.723576 48-49 TATGCCG 12840 0.0 36.012917 48-49 CTGCTTG 13170 0.0 35.672455 58-59 TGCTTGA 12860 0.0 35.31495 60-61 CGTATGC 13155 0.0 35.291893 46-47 TCGTATG 13395 0.0 35.013626 44-45 GCTTGAA 12930 0.0 34.811466 60-61 >>END_MODULE