##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34211-1.3410000003589a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3702924 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68289168235697 33.0 33.0 33.0 33.0 33.0 2 32.69905836576716 33.0 33.0 33.0 33.0 33.0 3 32.72291869884448 33.0 33.0 33.0 33.0 33.0 4 36.657999191989894 37.0 37.0 37.0 37.0 37.0 5 36.61979505925587 37.0 37.0 37.0 37.0 37.0 6 36.480779243646374 37.0 37.0 37.0 37.0 37.0 7 36.42417856807215 37.0 37.0 37.0 37.0 37.0 8 36.347512668366946 37.0 37.0 37.0 37.0 37.0 9 36.345661158587106 37.0 37.0 37.0 37.0 37.0 10-11 36.286615928385245 37.0 37.0 37.0 35.0 37.0 12-13 36.26115780394089 37.0 37.0 37.0 33.0 37.0 14-15 38.001492469194616 40.0 37.0 40.0 35.0 40.0 16-17 37.97083277971679 40.0 37.0 40.0 33.0 40.0 18-19 37.956214332241224 40.0 37.0 40.0 33.0 40.0 20-21 37.85798817367032 40.0 37.0 40.0 33.0 40.0 22-23 37.7460065342956 40.0 37.0 40.0 33.0 40.0 24-25 37.69881180386095 40.0 37.0 40.0 33.0 40.0 26-27 37.60132573609396 40.0 37.0 40.0 33.0 40.0 28-29 37.43406548446579 40.0 37.0 40.0 33.0 40.0 30-31 37.337229308514026 40.0 37.0 40.0 33.0 40.0 32-33 37.17947735357248 40.0 37.0 40.0 33.0 40.0 34-35 37.06245442790616 40.0 37.0 40.0 33.0 40.0 36-37 36.98016310893769 40.0 37.0 40.0 33.0 40.0 38-39 36.83468132211193 37.0 37.0 40.0 33.0 40.0 40-41 36.703447059675 37.0 37.0 40.0 33.0 40.0 42-43 36.565896302489605 37.0 37.0 40.0 33.0 40.0 44-45 36.41477208281887 37.0 37.0 40.0 33.0 40.0 46-47 36.2857937402982 37.0 37.0 40.0 33.0 40.0 48-49 36.05572974222534 37.0 37.0 40.0 30.0 40.0 50-51 35.87853828487973 37.0 37.0 40.0 27.0 40.0 52-53 35.726413639599414 37.0 37.0 40.0 27.0 40.0 54-55 35.49845635503186 37.0 35.0 40.0 27.0 40.0 56-57 35.27663867797449 37.0 33.0 40.0 27.0 40.0 58-59 35.051239912026276 37.0 33.0 40.0 27.0 40.0 60-61 34.80292182610283 37.0 33.0 40.0 27.0 40.0 62-63 34.57420716709282 37.0 33.0 40.0 27.0 40.0 64-65 34.280961478010354 37.0 33.0 38.5 27.0 40.0 66-67 33.92574098199153 37.0 33.0 37.0 27.0 40.0 68-69 33.6360593682182 37.0 33.0 37.0 27.0 40.0 70-71 33.33523763922781 37.0 33.0 37.0 22.0 40.0 72-73 32.95733655349124 37.0 33.0 37.0 22.0 40.0 74-75 32.53088842763179 37.0 33.0 37.0 22.0 38.5 76-77 29.927850531093807 33.0 27.0 35.0 18.5 37.0 78-79 32.27734042070537 35.0 33.0 37.0 22.0 37.0 80-81 32.62950427824066 37.0 33.0 37.0 22.0 37.0 82-83 32.508032571016855 37.0 33.0 37.0 22.0 37.0 84-85 32.32979315805564 35.0 33.0 37.0 22.0 37.0 86-87 32.12011129583 33.0 33.0 37.0 22.0 37.0 88-89 31.910088216771396 33.0 33.0 37.0 22.0 37.0 90-91 31.706130749645418 33.0 33.0 37.0 22.0 37.0 92-93 31.515704751164215 33.0 33.0 37.0 22.0 37.0 94-95 31.38504233411218 33.0 33.0 37.0 15.0 37.0 96-97 31.222232754439467 33.0 33.0 37.0 15.0 37.0 98-99 30.89061144652172 33.0 27.0 37.0 15.0 37.0 100-101 29.08733084989052 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1722438552090182 2306 2 0.1594231650634157 2306 3 0.1619182192489106 2306 4 0.15987104261390073 2306 5 0.24075667942522472 2306 6 0.22570553497678247 2306 7 0.3151729294368977 2306 8 0.2752688378641821 2306 9 0.2352910215688695 2306 10-11 0.31339778233068216 2306 12-13 0.25023116620123886 2306 14-15 0.5595485119267138 2306 16-17 0.561272792227669 2306 18-19 0.5907028293748979 2306 20-21 0.5798603671958062 2306 22-23 0.5373457674130719 2306 24-25 0.5232648356533858 2306 26-27 0.5799950945836088 2306 28-29 0.43113835186306915 2306 30-31 0.5869482764559919 2306 32-33 0.6843217758113056 2306 34-35 0.7148980824574096 2306 36-37 0.6382465970707187 2306 38-39 0.6102885205024364 2306 40-41 0.5959538177777475 2306 42-43 0.7360102253897125 2306 44-45 0.8144063537100337 2306 46-47 0.8211236305301526 2306 48-49 0.7499548261097999 2306 50-51 0.7130971626422493 2306 52-53 0.6555436499506229 2306 54-55 0.6067821821382964 2306 56-57 0.7239370017025735 2306 58-59 0.5989077933682836 2306 60-61 0.6959293900865973 2306 62-63 0.6456673580669587 2306 64-65 0.7052194860120267 2306 66-67 0.6909036388605756 2306 68-69 0.6922149401693005 2306 70-71 0.723189196438561 2306 72-73 0.5785769789347768 2306 74-75 0.621547809613169 2306 76-77 0.09904399834314503 2306 78-79 0.5424379097756074 2306 80-81 0.6161759041997286 2306 82-83 0.7107102121442139 2306 84-85 0.5651046998515667 2306 86-87 0.5161411927256978 2306 88-89 0.5197301185892087 2306 90-91 0.5986471143882959 2306 92-93 0.5125632325968006 2306 94-95 0.6163758346589425 2306 96-97 0.5082786502282737 2306 98-99 0.4243418063480995 2306 100-101 0.22529093112240517 2307 1 0.08539359301786931 2307 2 0.0847340541432331 2307 3 0.10054586337997762 2307 4 0.1648613770927767 2307 5 0.24969191673043412 2307 6 0.2647734972923601 2307 7 0.13025780826607303 2307 8 0.25776109804075276 2307 9 0.2046818715422063 2307 10-11 0.2164656594711687 2307 12-13 0.26839679101608027 2307 14-15 0.5294284705982761 2307 16-17 0.5271650753644863 2307 18-19 0.49518285396457884 2307 20-21 0.5638206015384029 2307 22-23 0.4884909094703076 2307 24-25 0.5601732391018643 2307 26-27 0.5353815858142781 2307 28-29 0.5032206421101435 2307 30-31 0.5713279981186119 2307 32-33 0.608101550060276 2307 34-35 0.5312712345282762 2307 36-37 0.5632492819376935 2307 38-39 0.6211513997167515 2307 40-41 0.5229506181569619 2307 42-43 0.6265076479174141 2307 44-45 0.5907120322962811 2307 46-47 0.6985169579876356 2307 48-49 0.7508210197461054 2307 50-51 0.692755592013576 2307 52-53 0.723239145299317 2307 54-55 0.6345881458759308 2307 56-57 0.6262656146077177 2307 58-59 0.5889256431046093 2307 60-61 0.7176377431706769 2307 62-63 0.5978748742131899 2307 64-65 0.7615165174749663 2307 66-67 0.688686586807485 2307 68-69 0.7450977466514956 2307 70-71 0.36017055420357025 2307 72-73 0.4754708655852511 2307 74-75 0.4368559953096778 2307 76-77 0.08119287329130032 2307 78-79 0.4523722879233887 2307 80-81 0.48011425224736826 2307 82-83 0.5441950398683346 2307 84-85 0.6247154126559664 2307 86-87 0.5453640255751395 2307 88-89 0.5850841321875961 2307 90-91 0.6044842003632915 2307 92-93 0.6373028004998638 2307 94-95 0.7614327168062367 2307 96-97 0.7001036006045247 2307 98-99 0.5452668263457277 2307 100-101 0.3195649430913541 2201 1 0.09984001445040747 2201 2 0.07759066871059872 2201 3 0.05339013598104003 2201 4 0.09057922631943427 2201 5 0.1394100292176148 2201 6 0.2083730245963551 2201 7 0.28583419079925676 2201 8 0.24911471655344286 2201 9 0.2478152191897891 2201 10-11 0.18695767319590573 2201 12-13 0.25334459342601434 2201 14-15 0.4004535380180627 2201 16-17 0.42799983604991354 2201 18-19 0.442190106335957 2201 20-21 0.4416901983034265 2201 22-23 0.45491867589696255 2201 24-25 0.4267363175297163 2201 26-27 0.5156909690645861 2201 28-29 0.4794674886041719 2201 30-31 0.490936850970634 2201 32-33 0.5336814155823717 2201 34-35 0.4688231820534199 2201 36-37 0.48625730714815063 2201 38-39 0.5560646328916192 2201 40-41 0.5105285007538498 2201 42-43 0.6168064323249638 2201 44-45 0.6417905207507744 2201 46-47 0.5940996222819237 2201 48-49 0.5208959382184304 2201 50-51 0.49184235818707833 2201 52-53 0.48958198739871506 2201 54-55 0.4860430675608072 2201 56-57 0.559426845715592 2201 58-59 0.5775682972398997 2201 60-61 0.5838758186580222 2201 62-63 0.5348177059515891 2201 64-65 0.48887171359591264 2201 66-67 0.526254434225855 2201 68-69 0.5914276407640742 2201 70-71 0.5137153018139387 2201 72-73 0.5757444545267418 2201 74-75 0.461421981984401 2201 76-77 0.34497511578226536 2201 78-79 0.45315359825053037 2201 80-81 0.6375609900486836 2201 82-83 0.5528275741125697 2201 84-85 0.4591289395552991 2201 86-87 0.5792745391514558 2201 88-89 0.6807531869878218 2201 90-91 0.7221223135803037 2201 92-93 0.5786194061463483 2201 94-95 0.5952362319207545 2201 96-97 0.6796653141829374 2201 98-99 0.688186177069678 2201 100-101 0.49828919169921804 1101 1 0.13247513347451445 1101 2 0.12361327546511802 1101 3 0.13052855444443878 1101 4 0.15046445176527357 1101 5 0.16509825963272817 1101 6 0.14101545782860114 1101 7 0.14620844420146284 1101 8 0.23556163515069528 1101 9 0.11315830675109595 1101 10-11 0.16565694367677963 1101 12-13 0.18164743382561 1101 14-15 0.3286106793914598 1101 16-17 0.4099305968299589 1101 18-19 0.3050691834584285 1101 20-21 0.294402964961165 1101 22-23 0.3586126435769472 1101 24-25 0.3090148689069139 1101 26-27 0.26082402182488806 1101 28-29 0.27675179626317714 1101 30-31 0.36410767722747295 1101 32-33 0.47475639938139835 1101 34-35 0.39670882002059216 1101 36-37 0.42017621598532884 1101 38-39 0.4140025235617557 1101 40-41 0.45160026630129124 1101 42-43 0.409906208363914 1101 44-45 0.4712087778920022 1101 46-47 0.42158627019496464 1101 48-49 0.4945960205764095 1101 50-51 0.5015910273669064 1101 52-53 0.40461670794476845 1101 54-55 0.4051843502681223 1101 56-57 0.5400583105613208 1101 58-59 0.46029213760877496 1101 60-61 0.39709742014778016 1101 62-63 0.39649703351255283 1101 64-65 0.40978852032966273 1101 66-67 0.4568283362535013 1101 68-69 0.5080527627284965 1101 70-71 0.5924305172685749 1101 72-73 0.703014163026296 1101 74-75 0.36901222834155334 1101 76-77 0.05895147041018234 1101 78-79 0.4466644327870455 1101 80-81 0.38006590153944586 1101 82-83 0.47821694598300013 1101 84-85 0.4463630132327836 1101 86-87 0.5586342094854757 1101 88-89 0.7265342054474146 1101 90-91 0.6262176118610263 1101 92-93 0.48727101724697874 1101 94-95 0.5894737330361686 1101 96-97 0.5780915160170466 1101 98-99 0.7257430267524612 1101 100-101 0.5495759803376181 2304 1 0.09779714592509237 2304 2 0.0765841090229955 2304 3 0.07846207419710538 2304 4 0.11846259954749883 2304 5 0.1446100964619177 2304 6 0.16987692271730026 2304 7 0.14431790468315597 2304 8 0.15376546293435922 2304 9 0.16450665342718906 2304 10-11 0.1254783882868935 2304 12-13 0.10537617972648405 2304 14-15 0.2076438935681182 2304 16-17 0.33833259993960496 2304 18-19 0.29215854697812915 2304 20-21 0.3073579109614357 2304 22-23 0.38398463397255966 2304 24-25 0.3741269101187612 2304 26-27 0.4894807048781047 2304 28-29 0.44483337368208 2304 30-31 0.4214960900884108 2304 32-33 0.49603035795798434 2304 34-35 0.44016148966917257 2304 36-37 0.493588508084855 2304 38-39 0.5380957252439202 2304 40-41 0.38727342171792856 2304 42-43 0.5237571247925601 2304 44-45 0.5764510731251349 2304 46-47 0.6220664895869632 2304 48-49 0.5766502658076433 2304 50-51 0.5823636895906432 2304 52-53 0.6020377741276164 2304 54-55 0.6334833636827284 2304 56-57 0.6309139832776225 2304 58-59 0.6995767184946047 2304 60-61 0.6710498093689878 2304 62-63 0.5837973662331422 2304 64-65 0.6630934716191277 2304 66-67 0.633422730500449 2304 68-69 0.5477674653058315 2304 70-71 0.6354510693013182 2304 72-73 0.5901482352508083 2304 74-75 0.5575061238070163 2304 76-77 0.4043288098829798 2304 78-79 0.7215790571241527 2304 80-81 0.5078687281674732 2304 82-83 0.584752200992277 2304 84-85 0.5595644558868784 2304 86-87 0.5741016250229478 2304 88-89 0.6662638774571761 2304 90-91 0.6589932839386456 2304 92-93 0.6659234090628949 2304 94-95 0.7343069835386409 2304 96-97 0.5314800965356277 2304 98-99 0.7297350749899572 2304 100-101 0.6441554490624846 2202 1 0.0520494919547545 2202 2 0.013851066169749515 2202 3 0.0574943944264561 2202 4 0.04798414590391786 2202 5 0.09024699296693939 2202 6 0.08721816843733166 2202 7 0.11532329901996974 2202 8 -0.00869883569492913 2202 9 0.010466905669552773 2202 10-11 0.14113274235290874 2202 12-13 0.15385301991397426 2202 14-15 0.1448617365994025 2202 16-17 0.0946704654244428 2202 18-19 0.13642318422296285 2202 20-21 0.13051876252891503 2202 22-23 0.22071626538398448 2202 24-25 0.06929710170488335 2202 26-27 0.1823505072847027 2202 28-29 0.14861164465499144 2202 30-31 0.17607750673281686 2202 32-33 0.12627156807179318 2202 34-35 0.061642263939610586 2202 36-37 0.2384273878094021 2202 38-39 0.0774405417353421 2202 40-41 0.06810102295674625 2202 42-43 0.1780593581980625 2202 44-45 0.1038998829868163 2202 46-47 0.1287046881899485 2202 48-49 0.1449416008332065 2202 50-51 0.1296205303170197 2202 52-53 0.3002114177171791 2202 54-55 0.07900155263929065 2202 56-57 0.17162529582306263 2202 58-59 0.19452684501328577 2202 60-61 0.21306080343989464 2202 62-63 0.2430075073495317 2202 64-65 0.137070058503447 2202 66-67 0.202176149005993 2202 68-69 0.1482283647308762 2202 70-71 0.25410086487665495 2202 72-73 0.22622405729605077 2202 74-75 0.11940730765655161 2202 76-77 -0.04367613608363641 2202 78-79 0.13059373855960388 2202 80-81 0.15027477371033626 2202 82-83 0.24980939869912078 2202 84-85 0.28935949476919376 2202 86-87 0.21421144974294037 2202 88-89 0.1658293413777514 2202 90-91 0.3584706565884055 2202 92-93 0.2671097233693054 2202 94-95 0.45641037739047263 2202 96-97 0.22252850831320714 2202 98-99 0.10334615136476444 2202 100-101 0.02167904165914436 1102 1 0.04455978970840846 1102 2 0.038957746725053255 1102 3 0.08716080366039591 1102 4 0.07133322649345075 1102 5 0.10827685253075714 1102 6 0.06525158375286821 1102 7 0.12500760013067236 1102 8 0.13483091806072878 1102 9 0.08438844414471447 1102 10-11 0.06512566798636499 1102 12-13 0.09488895595014668 1102 14-15 0.14356519981834026 1102 16-17 0.17701647827103528 1102 18-19 0.15359009101669585 1102 20-21 0.1123578032955237 1102 22-23 0.11004466690818049 1102 24-25 0.1942250022223817 1102 26-27 0.21704704596529467 1102 28-29 0.24436237194601773 1102 30-31 0.21392748068895884 1102 32-33 0.23219599255384793 1102 34-35 0.14018046367275616 1102 36-37 0.2769681828571322 1102 38-39 0.13796504926320097 1102 40-41 0.20958024341900483 1102 42-43 0.13411124869585223 1102 44-45 0.12817738614479168 1102 46-47 0.04394973892397047 1102 48-49 0.14040049396355414 1102 50-51 0.18227788380259113 1102 52-53 0.22339698921943096 1102 54-55 0.2190232563843395 1102 56-57 0.21606610957798011 1102 58-59 0.20209914485364777 1102 60-61 0.050269002627729265 1102 62-63 0.26534092566376444 1102 64-65 0.24641657387210358 1102 66-67 0.12852246577758564 1102 68-69 0.25984823480933983 1102 70-71 0.041320338529352796 1102 72-73 -0.06441836096514919 1102 74-75 0.024431493064462018 1102 76-77 -0.11296836754514672 1102 78-79 0.07366846921301828 1102 80-81 0.16698339191555078 1102 82-83 0.1910158656852161 1102 84-85 0.13693673339076184 1102 86-87 0.139734992031471 1102 88-89 0.2283578543553375 1102 90-91 0.2835967379739266 1102 92-93 0.24989538165239011 1102 94-95 0.11733941037007156 1102 96-97 -0.028617213158103283 1102 98-99 0.052651984750479386 1102 100-101 0.2454048697240374 2305 1 0.04650722158760345 2305 2 0.029499741067482432 2305 3 0.030579251277380592 2305 4 0.07307590195977554 2305 5 0.07360704377025229 2305 6 0.05286194914386755 2305 7 0.09710607636114332 2305 8 8.190624023924897E-4 2305 9 0.029183476237570005 2305 10-11 0.030060477212899173 2305 12-13 0.006277810837737263 2305 14-15 0.009139947700312234 2305 16-17 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50-51 -0.18420811327583664 1213 52-53 -0.15740585531668927 1213 54-55 -0.90050035699727 1213 56-57 -0.8094414565035919 1213 58-59 -1.1976210783458043 1213 60-61 -0.4420545741745059 1213 62-63 -0.3069484487657732 1213 64-65 -0.5996933819607975 1213 66-67 -0.5441723862944841 1213 68-69 -2.5053930298213345E-4 1213 70-71 0.10803062353317472 1213 72-73 0.22224667717244984 1213 74-75 0.32219330040769734 1213 76-77 -2.0481228262605775 1213 78-79 -1.6028719750328975 1213 80-81 -1.082486348433921 1213 82-83 -0.6718241545487089 1213 84-85 -0.3854540281192982 1213 86-87 -0.25189378315300814 1213 88-89 -0.3027096089386063 1213 90-91 -1.54124286296722 1213 92-93 -0.5520124602751046 1213 94-95 -0.20852324971636804 1213 96-97 -0.1598266281511691 1213 98-99 -1.959632146344628 1213 100-101 -0.5415016749620669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 7.0 8 35.0 9 138.0 10 226.0 11 192.0 12 218.0 13 375.0 14 932.0 15 1992.0 16 3587.0 17 5572.0 18 7906.0 19 10452.0 20 13275.0 21 16866.0 22 20700.0 23 25096.0 24 30194.0 25 36551.0 26 44122.0 27 53948.0 28 66747.0 29 84807.0 30 101187.0 31 117369.0 32 149801.0 33 202548.0 34 289899.0 35 437894.0 36 681822.0 37 936836.0 38 360433.0 39 1197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3864605542268 24.62041067202968 14.974560582659644 16.018568191083883 2 36.927420561464494 21.91081364440942 28.520199185777628 12.641566608348453 3 43.078419632819056 18.973437026126554 22.34167040026617 15.606472940788219 4 35.71210211173656 20.02755120007864 28.117455286686955 16.142891401497845 5 38.219949158534035 17.64973400455807 27.870796889882833 16.259519947025062 6 34.55971551130944 18.346798367992427 30.45963136159424 16.633854759103887 7 40.17344131286518 19.151027674346004 21.332141842500683 19.343389170288127 8 34.47024567611974 26.64691470848443 20.18113253202064 18.70170708337519 9 34.50724346489423 21.613460065613012 23.301315392916518 20.57798107657624 10-11 28.88140829589661 28.655221629445993 27.817057642424952 14.646312432232445 12-13 28.417137375760344 28.093231187029495 26.84894423974135 16.64068719746881 14-15 27.244631539831765 28.6645499610578 27.128223533618296 16.962594965492137 16-17 27.143279203137844 28.018303913339835 27.315399937994943 17.523016945527374 18-19 25.736992100564876 28.327432139420665 27.98828114978356 17.94729461023089 20-21 25.797059296923187 28.3414134343562 28.172587393098 17.688939875622616 22-23 25.104052905217607 29.804392420692405 27.197236562241084 17.894318111848907 24-25 24.4596837526236 28.68568190975564 28.11033928862704 18.744295048993713 26-27 24.45886007922388 28.337538118524712 27.560287491722757 19.643314310528652 28-29 24.795499448543907 29.29077129317264 27.285801706975356 18.6279275513081 30-31 23.885866952710884 28.503663591259233 28.313570572877 19.29689888315288 32-33 23.944860872110795 29.11061636695757 27.455667467115173 19.488855293816453 34-35 23.550078262475814 29.196116366417456 28.3556994418465 18.89810592926023 36-37 23.598826710840772 28.24714040136125 28.37757787866803 19.776455009129947 38-39 23.968832662915567 29.217953963296484 27.575315663470533 19.237897710317412 40-41 23.193170150491962 29.63781035625379 27.782275727114964 19.38674376613928 42-43 23.265693135364746 28.90690130944128 28.20030208942938 19.62710346576459 44-45 22.979505654960388 28.401378433306117 28.333620720735198 20.285495190998297 46-47 23.69313220743087 28.161128304321537 27.614998181051664 20.53074130719593 48-49 22.89731646785275 29.037616766239044 28.1276580747204 19.93740869118781 50-51 23.330675289442073 28.253435594876297 28.34955730402778 20.066331811653846 52-53 22.86372403143107 28.28215474930319 27.595466042377197 21.258655176888542 54-55 23.04261665940288 28.42746610724482 28.532648074885024 19.997269158467276 56-57 22.604260817438355 28.185022277482098 28.462266970298895 20.74844993478065 58-59 22.418151703035587 28.626402359275165 28.26495062063869 20.690495317050562 60-61 22.88510244663288 28.368747109246023 28.464033112426275 20.282117331694817 62-63 22.366048490389087 28.461707257624013 28.187298837265047 20.984945414721853 64-65 22.772788264578526 28.517782365753103 28.320006957588955 20.389422412079412 66-67 22.34345759204593 29.329554042308338 27.636761236738543 20.69022712890719 68-69 22.20868337577336 29.640253894568268 27.44947313311149 20.701589596546878 70-71 22.07655392907937 29.46453259375336 27.719295686238276 20.73961779092899 72-73 22.090868237934842 29.499145605538146 27.578362039843647 20.831624116683365 74-75 21.787513448494543 29.566007878977395 27.85782450803668 20.788654164491383 76-77 22.006920994787304 29.18574775933503 27.80988295579076 20.997448290086908 78-79 21.838000193125414 29.48045888218202 27.717021303893635 20.96451962079893 80-81 21.764298563506845 29.31472543889996 27.88402514072318 21.036950856870014 82-83 21.882328667831153 29.42875684189035 27.506735217898072 21.182179272380424 84-85 21.68027187827001 29.445228484777203 27.732539603339358 21.141960033613433 86-87 21.925475469229227 28.975637520185458 27.68201220879316 21.416874801792147 88-89 21.835264758999166 29.362347917027687 27.465274115042188 21.337113208930962 90-91 21.793582803051034 29.045310874593692 27.66689204283462 21.494214279520655 92-93 21.829508153319498 29.030682891584036 27.50381221261509 21.63599674248137 94-95 21.543540044646758 29.13494777906892 27.731075070551576 21.590437105732747 96-97 21.636497016668645 28.848428559098167 27.730359740877752 21.78471468335544 98-99 21.523964032844038 29.077485541070924 27.51590093835292 21.882649487732113 100-101 21.642500345432545 28.868341374066503 27.625756693118536 21.863401587382413 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 5.5 2 46.0 3 102.5 4 91.0 5 45.0 6 26.5 7 27.0 8 29.0 9 32.0 10 33.5 11 31.5 12 40.0 13 48.0 14 63.0 15 82.0 16 108.0 17 144.0 18 208.0 19 360.5 20 609.0 21 958.0 22 1533.5 23 2439.0 24 3836.0 25 5785.0 26 8456.0 27 11971.5 28 16342.5 29 21916.0 30 28895.0 31 37140.5 32 46896.5 33 58491.0 34 71928.0 35 86736.5 36 103055.0 37 120230.0 38 136283.5 39 150451.0 40 163208.0 41 173476.0 42 180694.0 43 184469.5 44 185122.5 45 185449.0 46 184499.5 47 182262.5 48 178218.0 49 169614.0 50 158506.5 51 145036.5 52 128193.5 53 109916.0 54 91484.5 55 74705.5 56 60541.5 57 49167.0 58 39671.0 59 31619.0 60 25396.5 61 20052.0 62 15474.0 63 11888.0 64 9097.0 65 6968.5 66 5341.0 67 3984.5 68 3019.0 69 2386.0 70 1825.5 71 1404.5 72 1081.5 73 839.0 74 661.0 75 497.5 76 375.5 77 299.5 78 228.5 79 163.0 80 140.0 81 110.0 82 84.5 83 73.0 84 55.0 85 41.5 86 32.0 87 19.0 88 12.5 89 13.0 90 9.5 91 6.5 92 4.5 93 2.5 94 2.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17540192561338014 2 0.006751421309214016 3 0.0017013581699219318 4 0.0 5 0.0011072330947110986 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.4305116713170457E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.7005685236856064E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.3502842618428032E-5 38-39 2.4305116713170457E-4 40-41 5.401137047371213E-5 42-43 0.001269267206132235 44-45 6.616392883029735E-4 46-47 0.006346336030661175 48-49 0.004685486388594527 50-51 0.008817356229833505 52-53 0.004172378369094262 54-55 0.020753869104523884 56-57 0.011301879271624262 58-59 0.02079437763237917 60-61 0.010437697344044868 62-63 0.006251816132332178 64-65 0.013286797136533184 66-67 0.03163716025497688 68-69 0.033216992841332955 70-71 0.03682225182045324 72-73 0.031853205736871726 74-75 0.03514789933576817 76-77 0.03479682542768904 78-79 0.017999289210364568 80-81 0.0023494946156064774 82-83 0.0 84-85 0.006413850243753315 86-87 0.027059696607329774 88-89 0.028045404118475024 90-91 0.0260604862535661 92-93 0.03601208126334756 94-95 0.03239331944160885 96-97 0.0343917401491362 98-99 0.040278979530770816 100-101 0.028180432544659306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3702924.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.41469114373102 #Duplication Level Percentage of deduplicated Percentage of total 1 97.14520969274939 90.74789759541169 2 2.1524592183328792 4.021426261600851 3 0.36245037033941346 1.015745682005617 4 0.09624278182145175 0.3596195895465762 5 0.05315745619386544 0.2482843676168176 6 0.03991161652077821 0.2236998798201317 7 0.020928899881169433 0.1368546702864258 8 0.026665840056034104 0.19927849703380457 9 0.00741525542065941 0.062342441538549835 >10 0.08802122862568723 1.3503196370840045 >50 0.006317953491987894 0.39959782423559675 >100 0.0010794080937167995 0.16981335243172174 >500 1.1137453296931981E-4 0.07258085597228928 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.8903939982126936E-5 0.9925393454158691 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 36760 0.9927289893068288 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.37176242342538E-4 0.0 2.7005685236856064E-5 2 9.722046685268183E-4 0.0 2.7005685236856064E-5 3 0.001728363855158788 0.0 2.7005685236856064E-5 4 0.002133449133711629 0.0 2.7005685236856064E-5 5 0.0027815855793961743 0.0 2.7005685236856064E-5 6 0.004374921008370682 0.0 2.7005685236856064E-5 7 0.007048483846819433 0.0 5.401137047371213E-5 8 0.00993809216716303 0.0 5.401137047371213E-5 9 0.011531427596137539 0.0 8.101705571056818E-5 10-11 0.016162902614258352 0.0 8.101705571056818E-5 12-13 0.0211454515404583 0.0 8.101705571056818E-5 14-15 0.02499376168671029 0.0 1.2152558356585228E-4 16-17 0.02805890696109345 0.0 1.350284261842803E-4 18-19 0.03252834786779313 0.0 1.350284261842803E-4 20-21 0.03811852471182234 0.0 1.4853126880270834E-4 22-23 0.04432983231629922 0.0 1.7553695403956443E-4 24-25 0.051081253625513245 0.0 1.8903979665799245E-4 26-27 0.05696849300714786 0.0 2.160454818948485E-4 28-29 0.06295025228711149 0.0 2.160454818948485E-4 30-31 0.09137373599890249 0.0 2.2954832451327654E-4 32-33 0.10004256095993329 0.0 2.4305116713170457E-4 34-35 0.10610533729560748 0.0 2.4305116713170457E-4 36-37 0.11361291779145347 0.0 2.8355969498698865E-4 38-39 0.1219711773722604 0.0 3.2406822284227274E-4 40-41 0.13153118994610746 0.0 3.510739080791288E-4 42-43 0.14245498962441572 0.0 3.510739080791288E-4 44-45 0.15487760483336951 0.0 3.510739080791288E-4 46-47 0.16840745313703442 0.0 3.510739080791288E-4 48-49 0.18349012834181852 0.0 3.780795933159849E-4 50-51 0.20136789196861724 0.0 4.32090963789697E-4 52-53 0.22109554503414058 0.0 4.590966490265531E-4 54-55 0.24257856764005958 2.7005685236856064E-5 4.725994916449811E-4 56-57 0.26699170709417747 2.7005685236856064E-5 4.8610233426340914E-4 58-59 0.29376784400652023 2.7005685236856064E-5 4.996051768818372E-4 60-61 0.32346059492444346 2.7005685236856064E-5 5.131080195002652E-4 62-63 0.3555973603563022 2.7005685236856064E-5 5.401137047371213E-4 64-65 0.3893544669023723 2.7005685236856064E-5 5.401137047371213E-4 66-67 0.42540705669357515 2.7005685236856064E-5 5.536165473555493E-4 68-69 0.4650784083065167 2.7005685236856064E-5 5.941250752108334E-4 70-71 0.5079364307774072 2.7005685236856064E-5 6.211307604476894E-4 72-73 0.5558580192302083 2.7005685236856064E-5 7.021478161582576E-4 74-75 0.6068312501147741 2.7005685236856064E-5 7.291535013951137E-4 76-77 0.660518552365644 2.7005685236856064E-5 7.561591866319698E-4 78-79 0.7206737162307408 2.7005685236856064E-5 7.831648718688259E-4 80-81 0.7855953835401428 2.7005685236856064E-5 8.101705571056819E-4 82-83 0.8540277899303361 2.7005685236856064E-5 8.101705571056819E-4 84-85 0.9265650604765314 2.7005685236856064E-5 8.2367339972411E-4 86-87 1.0038958401522688 2.7005685236856064E-5 8.911876128162501E-4 88-89 1.0887612060090892 2.7005685236856064E-5 9.181932980531062E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCGG 40 5.3926324E-4 47.494835 2 TCGGAAG 9875 0.0 44.678806 3 CGGAAGA 9960 0.0 44.345192 4 GATCGGA 10265 0.0 43.156597 1 ATCGGAA 10315 0.0 42.821323 2 AGAGCAC 11145 0.0 39.586475 8 GCCGTCT 5500 0.0 38.996315 50-51 TGCCGTC 5435 0.0 38.98197 50-51 GAGCACA 11240 0.0 38.91388 9 ATGCCGT 5550 0.0 38.47277 48-49 CGTATGC 5555 0.0 38.26505 46-47 GAAGAGC 11550 0.0 38.15726 6 TATGCCG 5605 0.0 37.883377 48-49 TCTCGTA 5510 0.0 37.844288 42-43 TCGTATG 5740 0.0 37.527775 44-45 CTCGTAT 5590 0.0 37.515118 44-45 CGTCTTC 5740 0.0 37.200287 52-53 AATCTCG 5565 0.0 36.99832 40-41 CCGTCTT 5790 0.0 36.632908 52-53 CGCGGCC 130 2.0190782E-10 36.602703 1 >>END_MODULE