FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34211-1.3410000003589a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34211-1.3410000003589a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702924
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT367600.9927289893068288TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG405.3926324E-447.4948352
TCGGAAG98750.044.6788063
CGGAAGA99600.044.3451924
GATCGGA102650.043.1565971
ATCGGAA103150.042.8213232
AGAGCAC111450.039.5864758
GCCGTCT55000.038.99631550-51
TGCCGTC54350.038.9819750-51
GAGCACA112400.038.913889
ATGCCGT55500.038.4727748-49
CGTATGC55550.038.2650546-47
GAAGAGC115500.038.157266
TATGCCG56050.037.88337748-49
TCTCGTA55100.037.84428842-43
TCGTATG57400.037.52777544-45
CTCGTAT55900.037.51511844-45
CGTCTTC57400.037.20028752-53
AATCTCG55650.036.9983240-41
CCGTCTT57900.036.63290852-53
CGCGGCC1302.0190782E-1036.6027031
Produced by FastQC (version 0.11.2)