##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34139-1.341000000356ec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2741610 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69271304087744 33.0 33.0 33.0 33.0 33.0 2 32.706007418998325 33.0 33.0 33.0 33.0 33.0 3 32.7386944167843 33.0 33.0 33.0 33.0 33.0 4 36.66741987372384 37.0 37.0 37.0 37.0 37.0 5 36.636315522630866 37.0 37.0 37.0 37.0 37.0 6 36.52604236196979 37.0 37.0 37.0 37.0 37.0 7 36.39921505976415 37.0 37.0 37.0 37.0 37.0 8 36.352988207659 37.0 37.0 37.0 37.0 37.0 9 36.41017759637585 37.0 37.0 37.0 37.0 37.0 10-11 36.32206422503565 37.0 37.0 37.0 37.0 37.0 12-13 36.31668052713552 37.0 37.0 37.0 37.0 37.0 14-15 38.158140289829696 40.0 37.0 40.0 37.0 40.0 16-17 38.17969915487615 40.0 37.0 40.0 37.0 40.0 18-19 38.12472379368327 40.0 37.0 40.0 37.0 40.0 20-21 38.107488665419226 40.0 37.0 40.0 37.0 40.0 22-23 37.90338687851299 40.0 37.0 40.0 33.0 40.0 24-25 37.986024452785045 40.0 37.0 40.0 35.0 40.0 26-27 37.92702189589329 40.0 37.0 40.0 33.0 40.0 28-29 37.71690703637644 40.0 37.0 40.0 33.0 40.0 30-31 37.63033363607515 40.0 37.0 40.0 33.0 40.0 32-33 37.5552719022764 40.0 37.0 40.0 33.0 40.0 34-35 37.42998584773181 40.0 37.0 40.0 33.0 40.0 36-37 37.3968217580181 40.0 37.0 40.0 33.0 40.0 38-39 37.35662621598258 40.0 37.0 40.0 33.0 40.0 40-41 37.289854319177415 40.0 37.0 40.0 33.0 40.0 42-43 36.87563986124941 37.0 37.0 40.0 33.0 40.0 44-45 36.727082261882614 37.0 37.0 40.0 33.0 40.0 46-47 36.90524272234198 37.0 37.0 40.0 33.0 40.0 48-49 36.63997924577164 37.0 37.0 40.0 33.0 40.0 50-51 36.553659346150624 37.0 37.0 40.0 33.0 40.0 52-53 36.49145155583763 37.0 37.0 40.0 33.0 40.0 54-55 36.19908575617976 37.0 37.0 40.0 33.0 40.0 56-57 36.0673651613468 37.0 37.0 40.0 33.0 40.0 58-59 35.89665342627143 37.0 37.0 40.0 30.0 40.0 60-61 35.614741702868024 37.0 35.0 40.0 27.0 40.0 62-63 35.29072461072144 37.0 33.0 38.5 27.0 40.0 64-65 34.987366729768276 37.0 33.0 37.0 27.0 40.0 66-67 34.426290391412344 37.0 33.0 37.0 27.0 40.0 68-69 34.19617341635025 37.0 33.0 37.0 27.0 40.0 70-71 33.82103563234742 37.0 33.0 37.0 27.0 40.0 72-73 32.943301746054324 37.0 33.0 37.0 22.0 37.0 74-75 32.113343801634805 37.0 33.0 37.0 18.5 37.0 76-77 29.490611720850154 33.0 27.0 35.0 18.5 37.0 78-79 31.616341857521675 33.0 33.0 37.0 18.5 37.0 80-81 31.909536914440785 37.0 33.0 37.0 15.0 37.0 82-83 31.823759943974526 37.0 33.0 37.0 15.0 37.0 84-85 31.664069105379685 35.0 33.0 37.0 15.0 37.0 86-87 31.48969273529058 33.0 33.0 37.0 15.0 37.0 88-89 31.34346497131248 33.0 33.0 37.0 15.0 37.0 90-91 31.189830428106113 33.0 33.0 37.0 15.0 37.0 92-93 31.025642596868266 33.0 33.0 37.0 15.0 37.0 94-95 30.93986234365938 33.0 33.0 37.0 15.0 37.0 96-97 30.801328416514384 33.0 33.0 37.0 15.0 37.0 98-99 30.517077738992782 33.0 30.0 37.0 15.0 37.0 100-101 28.976290391412345 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1492224489071532 2306 2 0.1705334463954742 2306 3 0.14901404052489653 2306 4 0.18038938573093333 2306 5 0.21261372623689567 2306 6 0.2499946478940558 2306 7 0.2686581252175415 2306 8 0.28552976121809337 2306 9 0.231826264294682 2306 10-11 0.3118366505322214 2306 12-13 0.26369032953824245 2306 14-15 0.5159018165757203 2306 16-17 0.5740011127029803 2306 18-19 0.5921557170889997 2306 20-21 0.5931736894884097 2306 22-23 0.5627230235790037 2306 24-25 0.584265660488775 2306 26-27 0.6206474583795227 2306 28-29 0.6372959503893725 2306 30-31 0.6263679229234569 2306 32-33 0.6330855867465246 2306 34-35 0.6865013388748338 2306 36-37 0.7156188935178847 2306 38-39 0.8071296832441917 2306 40-41 0.7345799156737627 2306 42-43 0.7513202787686168 2306 44-45 0.8569886835444933 2306 46-47 0.845251283332864 2306 48-49 0.8847710121640162 2306 50-51 0.8367374636279123 2306 52-53 0.9684302452740141 2306 54-55 0.9120589497923604 2306 56-57 0.8282595678704538 2306 58-59 0.7679349337264014 2306 60-61 0.713521181889206 2306 62-63 0.8227918188619441 2306 64-65 0.7296198293982386 2306 66-67 0.7135162616321722 2306 68-69 1.0176401283312586 2306 70-71 0.8470066667623541 2306 72-73 0.6800154993154806 2306 74-75 0.47800194690363185 2306 76-77 0.28184627107524207 2306 78-79 0.7204396512903202 2306 80-81 0.8596443424091404 2306 82-83 0.9592441634016353 2306 84-85 0.7615255696939869 2306 86-87 0.8544355327679227 2306 88-89 0.9621790915505706 2306 90-91 0.9707214211289674 2306 92-93 0.9977853316661971 2306 94-95 0.9849656495257015 2306 96-97 0.9551669481637859 2306 98-99 0.879192307482004 2306 100-101 0.62866222200811 2307 1 0.08767981620347598 2307 2 0.03865720777766768 2307 3 0.13235120550999824 2307 4 0.163210490274885 2307 5 0.17584247972782663 2307 6 0.1986364485957779 2307 7 0.246185245066755 2307 8 0.3220468576602826 2307 9 0.21136330375681922 2307 10-11 0.3150174065219673 2307 12-13 0.2491147747005158 2307 14-15 0.570846005415035 2307 16-17 0.4468019143427284 2307 18-19 0.44635675949228926 2307 20-21 0.53585266400259 2307 22-23 0.5336075628155967 2307 24-25 0.5114097121646637 2307 26-27 0.6221044560912006 2307 28-29 0.5407863426438126 2307 30-31 0.586848557561396 2307 32-33 0.5997657369472833 2307 34-35 0.5522368656590473 2307 36-37 0.5445286091317243 2307 38-39 0.6214352278868134 2307 40-41 0.6206937671434218 2307 42-43 0.650082946257875 2307 44-45 0.5794032158841205 2307 46-47 0.6430710249728264 2307 48-49 0.5960245249156628 2307 50-51 0.5224583525323254 2307 52-53 0.5054587944748548 2307 54-55 0.60098654321434 2307 56-57 0.651408952817583 2307 58-59 0.5511553545872729 2307 60-61 0.6199573383253636 2307 62-63 0.4232526356148938 2307 64-65 0.3507104532978573 2307 66-67 0.5256906318589714 2307 68-69 0.5864534415265936 2307 70-71 0.5027659788887036 2307 72-73 0.2289197439126056 2307 74-75 0.0631123906283122 2307 76-77 -0.012567665660792926 2307 78-79 0.28750083258371006 2307 80-81 0.4361777063956822 2307 82-83 0.4256022608458778 2307 84-85 0.42904538515387713 2307 86-87 0.5337175491285961 2307 88-89 0.5165924268627755 2307 90-91 0.5418252375511372 2307 92-93 0.5451964446203803 2307 94-95 0.6265214165496324 2307 96-97 0.5828305524191499 2307 98-99 0.2514413997685523 2307 100-101 0.10517225657057949 2201 1 0.05692763855864058 2201 2 0.061148408234970475 2201 3 0.08174149105847306 2201 4 0.13806844253396378 2201 5 0.19532393423752836 2201 6 0.13633664335812057 2201 7 0.23472028162439074 2201 8 0.20552464226800993 2201 9 0.20266860297171974 2201 10-11 0.26032744928963325 2201 12-13 0.21941971675686744 2201 14-15 0.4598476754901526 2201 16-17 0.4229464639336058 2201 18-19 0.39052909349513243 2201 20-21 0.34954488729933075 2201 22-23 0.4267619079473448 2201 24-25 0.40616866059593093 2201 26-27 0.28109612920716387 2201 28-29 0.2778473932641816 2201 30-31 0.4200647594216633 2201 32-33 0.37723072910153377 2201 34-35 0.4278094177852765 2201 36-37 0.45071369469533806 2201 38-39 0.3800478170078776 2201 40-41 0.3368119931486504 2201 42-43 0.6044302748989949 2201 44-45 0.6400160886438826 2201 46-47 0.4496138781258523 2201 48-49 0.5295366985408307 2201 50-51 0.3573585504268664 2201 52-53 0.47993337770649447 2201 54-55 0.5028753942557316 2201 56-57 0.507783580378657 2201 58-59 0.39743220510141697 2201 60-61 0.5011778678509842 2201 62-63 0.586896070444773 2201 64-65 0.5635060389747935 2201 66-67 0.45835297695069954 2201 68-69 0.6299659892764282 2201 70-71 0.4159734062204308 2201 72-73 0.3665761965408123 2201 74-75 0.3326530090389852 2201 76-77 0.16479298486369842 2201 78-79 0.7008129930710112 2201 80-81 0.6012930607765767 2201 82-83 0.7266352582065565 2201 84-85 0.5873394897756654 2201 86-87 0.5897204229280746 2201 88-89 0.779068070694354 2201 90-91 0.7831279265939379 2201 92-93 0.7648513450662406 2201 94-95 0.8874931662219474 2201 96-97 0.7616503979056652 2201 98-99 0.6830103002640051 2201 100-101 0.4538200134104251 1101 1 0.0994221769654402 1101 2 0.05322063998815452 1101 3 0.10834695993712273 1101 4 0.120196133535039 1101 5 0.1593741260045647 1101 6 0.07187816693554083 1101 7 0.1129803961756366 1101 8 0.15670972565648356 1101 9 0.10213476903808782 1101 10-11 0.10994785733477386 1101 12-13 0.14314304127780275 1101 14-15 0.31680153862547655 1101 16-17 0.36638741235960026 1101 18-19 0.32098387529588024 1101 20-21 0.33984685427128625 1101 22-23 0.40991614892811157 1101 24-25 0.41123095120158837 1101 26-27 0.3763975123450436 1101 28-29 0.4123298318343558 1101 30-31 0.40420233667988725 1101 32-33 0.36776193827681425 1101 34-35 0.46798738238995696 1101 36-37 0.4753844272424388 1101 38-39 0.5298481657115133 1101 40-41 0.5193558530604037 1101 42-43 0.4937425183488173 1101 44-45 0.6107394540831095 1101 46-47 0.5192818511836563 1101 48-49 0.6332850131565451 1101 50-51 0.562777329125808 1101 52-53 0.5910601575271954 1101 54-55 0.5758818004625397 1101 56-57 0.6381024336287169 1101 58-59 0.7429519022661779 1101 60-61 0.6375457412078873 1101 62-63 0.6752553170293396 1101 64-65 0.5809098834972843 1101 66-67 0.5414383554498343 1101 68-69 0.5608710718265755 1101 70-71 0.5624553817153668 1101 72-73 0.4601777741902495 1101 74-75 0.48339338051713554 1101 76-77 0.4537945487406354 1101 78-79 0.8575555781286539 1101 80-81 0.8183351277687905 1101 82-83 0.7492182599520731 1101 84-85 0.7671326428489778 1101 86-87 0.8548253084716464 1101 88-89 0.9645059512468954 1101 90-91 0.9778371921905453 1101 92-93 0.9577208845958047 1101 94-95 0.9376101825403644 1101 96-97 0.9180185307443871 1101 98-99 0.8927407685188982 1101 100-101 0.9001473976241527 2304 1 -0.0392945876118489 2304 2 0.04056334922849203 2304 3 0.03725392463419297 2304 4 0.05250094603526634 2304 5 0.09942306294865233 2304 6 0.043109158863885 2304 7 0.11012574110393558 2304 8 0.18089309958691047 2304 9 0.18705469541223607 2304 10-11 0.15185434074507498 2304 12-13 0.14813271749304846 2304 14-15 0.32744743648245844 2304 16-17 0.23785261826583337 2304 18-19 0.3390200967171779 2304 20-21 0.3288361337005057 2304 22-23 0.304005598460968 2304 24-25 0.24448244741559222 2304 26-27 0.39073067300297026 2304 28-29 0.38214240554287215 2304 30-31 0.32198257600064295 2304 32-33 0.34671234737056267 2304 34-35 0.3669595963458079 2304 36-37 0.2873684676264432 2304 38-39 0.44578555434506484 2304 40-41 0.4555232673748293 2304 42-43 0.45111116563910514 2304 44-45 0.4163486492320274 2304 46-47 0.3477872537194102 2304 48-49 0.506804413421797 2304 50-51 0.39013085813343906 2304 52-53 0.38371897297393076 2304 54-55 0.31713661792259273 2304 56-57 0.46545926918661706 2304 58-59 0.5265328594757435 2304 60-61 0.6528704077055494 2304 62-63 0.3514059965254219 2304 64-65 0.45877978543969533 2304 66-67 0.3876817469417162 2304 68-69 0.512160167191162 2304 70-71 0.43110450896978847 2304 72-73 0.39154251607725854 2304 74-75 0.33585777080764245 2304 76-77 0.166597419561068 2304 78-79 0.5404671426045482 2304 80-81 0.48803188036902867 2304 82-83 0.3414343650562799 2304 84-85 0.317311104715273 2304 86-87 0.4395761415386161 2304 88-89 0.3978933834166831 2304 90-91 0.39191427889931063 2304 92-93 0.41102706747742346 2304 94-95 0.4609017063824261 2304 96-97 0.26142251403528505 2304 98-99 0.38862392972792037 2304 100-101 0.5004361696815955 2202 1 0.0731041996378039 2202 2 0.08209042285267287 2202 3 0.10865912460398874 2202 4 0.07762854399641839 2202 5 0.09981658778153246 2202 6 0.10459885953331849 2202 7 0.10660000349146515 2202 8 0.08793348673099644 2202 9 0.1830803326877728 2202 10-11 0.15873167187802295 2202 12-13 0.10453946843366424 2202 14-15 0.2057197808360769 2202 16-17 0.28758912328914477 2202 18-19 0.2435247656155255 2202 20-21 0.1604274875823819 2202 22-23 0.27896447732674545 2202 24-25 0.2524161223253003 2202 26-27 0.34831842359870535 2202 28-29 0.17635417224148853 2202 30-31 0.32632138285511303 2202 32-33 0.2670612375761081 2202 34-35 0.2130255724676573 2202 36-37 0.30076599845368435 2202 38-39 0.34223869495893666 2202 40-41 0.21359732895245287 2202 42-43 0.375534670465818 2202 44-45 0.2923089076455341 2202 46-47 0.31529577555578925 2202 48-49 0.3604964212283548 2202 50-51 0.4374926941835753 2202 52-53 0.3768915552110954 2202 54-55 0.47459034219127005 2202 56-57 0.45250568675013625 2202 58-59 0.4898457671582861 2202 60-61 0.4195976790104794 2202 62-63 0.5515859057392092 2202 64-65 0.587186309176694 2202 66-67 0.591836954224199 2202 68-69 0.5120124678318874 2202 70-71 0.3331351923424961 2202 72-73 0.15409645142762685 2202 74-75 0.08215219200641855 2202 76-77 0.1222662689243883 2202 78-79 0.3090304946606395 2202 80-81 0.2738749520531343 2202 82-83 0.4602587121689936 2202 84-85 0.4655777077558554 2202 86-87 0.3745092593232897 2202 88-89 0.3859215723113252 2202 90-91 0.43178341233518225 2202 92-93 0.2156960678563209 2202 94-95 0.3578782996478118 2202 96-97 0.34071453667129603 2202 98-99 0.2511761134336119 2202 100-101 0.433575382237386 1102 1 0.04203618933056674 1102 2 0.030777219025452496 1102 3 0.018044866133521964 1102 4 0.048952469870471305 1102 5 0.08259449295777443 1102 6 0.07811040394323499 1102 7 0.13782649886052667 1102 8 0.03601489157220783 1102 9 0.09478270298851044 1102 10-11 0.04224445700838686 1102 12-13 0.027186644266897986 1102 14-15 0.1250215638624681 1102 16-17 0.16280497623969126 1102 18-19 0.07274229693368284 1102 20-21 0.10649318465586077 1102 22-23 0.17732925716213543 1102 24-25 0.11604589936907672 1102 26-27 0.02613789136666611 1102 28-29 -0.024440999936246044 1102 30-31 8.3951573751051E-4 1102 32-33 0.019775313549565965 1102 34-35 -0.04570260265787596 1102 36-37 0.03283650779412284 1102 38-39 0.21552425541118936 1102 40-41 0.14161424719422655 1102 42-43 -0.017298332574561925 1102 44-45 0.15299791489325543 1102 46-47 0.03604486515401106 1102 48-49 0.06424208526532738 1102 50-51 -0.04387396864569837 1102 52-53 -0.0034357110404172886 1102 54-55 0.04724131425870581 1102 56-57 -0.015372153519756182 1102 58-59 -0.041616526822707556 1102 60-61 -0.1772070646156294 1102 62-63 0.05967180742045031 1102 64-65 0.08533194136234101 1102 66-67 0.037825414906791366 1102 68-69 0.2165588132157481 1102 70-71 0.043772255995470744 1102 72-73 -0.1894168336945654 1102 74-75 -0.12425114460566533 1102 76-77 -0.3269583270930241 1102 78-79 0.08999194806896327 1102 80-81 -0.10386400777252547 1102 82-83 -0.05699764135361818 1102 84-85 0.04974145445999412 1102 86-87 -0.020989518011525377 1102 88-89 -0.026757865339849474 1102 90-91 -0.0669745555128749 1102 92-93 -0.07214436253169865 1102 94-95 -0.13175929270021314 1102 96-97 -0.019429649241711644 1102 98-99 -0.20501248903677904 1102 100-101 -0.15271826789734888 2305 1 -0.023819859057894632 2305 2 -0.050524577120512504 2305 3 0.017728989459911304 2305 4 -0.005216989206267897 2305 5 0.08048224428880957 2305 6 -0.01725722790843065 2305 7 0.018428789220692465 2305 8 -0.0823323444786439 2305 9 -0.04371678674917234 2305 10-11 -0.05601734542121051 2305 12-13 0.0038518907128661795 2305 14-15 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32 83618.0 33 118343.0 34 179863.0 35 305704.0 36 558138.0 37 786347.0 38 205067.0 39 442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23826780756698 24.490113585790045 18.311479231478092 14.960139375164882 2 29.441268296490787 28.827394060277058 27.466978133032953 14.2643595101992 3 35.70169900052999 18.494772853196707 28.91857279432497 16.88495535194833 4 29.81620288808401 18.447882813383377 26.840834400224683 24.89507989830793 5 38.316785132115086 16.603886726921306 27.413100514410083 17.66622762655353 6 35.17907361003206 17.393137608923222 29.65388950288334 17.773899278161373 7 32.051203489920155 25.25541561345341 22.98518024080741 19.708200655819024 8 28.67803954610612 31.61904866118813 20.83403547550527 18.868876317200478 9 34.81286543308494 22.10857853596974 23.358938725785215 19.719617305160106 10-11 26.40683000882329 30.10818107774017 27.206324611806142 16.278664301630393 12-13 22.48545562643848 29.25979625110793 26.854895481122405 21.399852641331186 14-15 22.38555082597452 29.516780286036308 26.97548156010519 21.12218732788398 16-17 25.97471923431852 25.51945754501917 27.32855876656417 21.17726445409814 18-19 21.157590094431754 25.61008414947497 31.44497784969012 21.787347906403156 20-21 24.765812788835756 25.72441010938828 31.3329576416777 18.176819460098265 22-23 20.549749964436955 33.61920550333563 27.34914885778794 18.48189567443947 24-25 20.643745828181252 25.147650468155575 31.649359318064935 22.55924438559824 26-27 20.64163028293594 25.324462633270233 27.67578174868052 26.358125335113307 28-29 24.24278070185037 29.992650304018444 27.228143317247895 18.53642567688329 30-31 19.7513687212988 27.00508095608055 30.941563533835957 22.301986788784692 32-33 19.403106204018805 31.28123620792162 27.230532424378378 22.085125163681194 34-35 19.654090114932465 30.997971994557943 31.563387936285615 17.784549954223976 36-37 19.515091320461817 31.157962349846386 31.77380509036847 17.55314123932333 38-39 19.731970773313726 27.00959000643419 31.335342376214797 21.92309684403729 40-41 23.77054427335476 26.484223956767 27.22557680078071 22.519654969097534 42-43 19.98712055611768 30.340245654145164 31.01649852749037 18.656135262246778 44-45 19.473605789037805 26.454923784343826 31.527626887336226 22.543843539282143 46-47 23.54639286568889 26.23819240578729 27.598655604904838 22.616759123618984 48-49 19.477746807365513 30.962841402287903 30.951369603307377 18.6080421870392 50-51 23.513633989255062 26.81528746064032 30.443673706419823 19.227404843684795 52-53 19.45694161246904 26.676101856276286 26.693501026806395 27.17345550444828 54-55 23.780828934357288 26.444748745336454 30.869237909891872 18.905184410414385 56-57 19.891887790492056 26.435746156457196 30.519766309533168 23.152599743517584 58-59 19.74508583205996 26.871840090737976 30.646557314371947 22.73651676283012 60-61 23.30767701491775 26.887244128381298 30.729508839574443 19.075570017126505 62-63 19.43484693512781 26.969066027783818 30.41665511561027 23.17943192147811 64-65 23.485131062498983 26.859481294882276 30.47501549009154 19.180372152527198 66-67 19.951415255084505 34.34705401167114 26.500395143276833 19.201135589967517 68-69 20.193335281718635 34.39611548889694 26.454329155250917 18.956220074133505 70-71 19.72915558338387 34.82991402656485 26.510482687456165 18.930447702595114 72-73 19.61593197810319 34.6991687903138 26.366475485215897 19.318423746367117 74-75 19.975666551852978 33.819388091679706 26.414780754501088 19.790164601966236 76-77 20.307770053627152 32.73325166267892 26.501592212888582 20.457386070805356 78-79 20.031731456588044 32.245569662275955 27.06416353702499 20.658535344111012 80-81 19.629362748826075 31.856594913422104 27.374730011159755 21.13931232659207 82-83 19.312538982568636 31.703652233541607 27.23666021060618 21.74714857328358 84-85 19.37273669878795 31.38061943892472 27.75271687338645 21.493926988900874 86-87 19.535031270818937 31.175649258964413 28.192078934829297 21.09724053538736 88-89 19.869486267271487 31.455681262201914 27.93113185966016 20.74370061086644 90-91 20.319056581091527 30.910355246924897 27.372260709844433 21.398327462139143 92-93 19.97972381404654 31.12410963297622 27.219785354113533 21.6763811988637 94-95 19.652558923142458 31.235709900839275 27.389722348150734 21.72200882786753 96-97 19.811278760349968 31.704259737170197 27.050901792200488 21.433559710279347 98-99 20.04389292155942 31.236806854724975 26.963094405358373 21.756205818357234 100-101 20.093539608394558 31.2072330292086 26.717328177794574 21.981899184602266 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 66.0 1 48.0 2 29.5 3 42.5 4 42.0 5 30.5 6 32.0 7 43.5 8 51.0 9 47.0 10 53.0 11 65.5 12 76.5 13 70.0 14 76.0 15 104.5 16 121.0 17 129.5 18 157.5 19 209.0 20 291.0 21 420.5 22 582.0 23 891.5 24 1343.5 25 1931.0 26 2787.5 27 3928.5 28 5501.5 29 7346.5 30 9621.5 31 12880.5 32 18484.0 33 28172.0 34 42512.5 35 63304.0 36 92768.5 37 130164.5 38 166168.5 39 188884.0 40 200169.0 41 196332.0 42 184022.5 43 176514.0 44 163479.0 45 149764.0 46 144556.0 47 133561.0 48 112555.5 49 92909.5 50 76346.5 51 64066.0 52 54898.5 53 45709.0 54 37824.5 55 30511.5 56 23970.5 57 18273.0 58 13776.0 59 10491.0 60 7992.0 61 6237.0 62 4809.0 63 3513.0 64 2565.5 65 1880.5 66 1368.0 67 1014.5 68 743.0 69 572.5 70 443.5 71 325.0 72 230.0 73 159.5 74 122.0 75 103.5 76 86.5 77 65.0 78 46.0 79 40.0 80 29.5 81 19.5 82 15.5 83 12.5 84 12.5 85 10.0 86 5.5 87 4.0 88 4.5 89 3.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1759550045411273 2 0.006164261145823075 3 0.002079070327289439 4 0.0 5 8.389231145203001E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.0942475406786523E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.8237459011310872E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.6474918022621744E-5 38-39 2.1884950813573045E-4 40-41 1.8237459011310872E-5 42-43 0.0011671973767238958 44-45 5.288863113280152E-4 46-47 0.005617137375483749 48-49 0.003811628933363972 50-51 0.008243331473112514 52-53 0.0036474918022621743 54-55 0.019568793519136568 56-57 0.010632438603594238 58-59 0.019349944011000837 60-61 0.008936354915542328 62-63 0.005763037047574236 64-65 0.012456184504725326 66-67 0.03034713179482129 68-69 0.03206145294188451 70-71 0.036329018350531254 72-73 0.030839543188126685 74-75 0.034213473105219196 76-77 0.03383048646598167 78-79 0.01756267302789237 80-81 0.002243207458391237 82-83 0.0 84-85 0.00647429794901536 86-87 0.02611604130419717 88-89 0.027319713598943685 90-91 0.025003556304507207 92-93 0.0348882590886377 94-95 0.03127724220439815 96-97 0.032863901138382196 98-99 0.03928348671036362 100-101 0.02695496441871747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2741610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.12668304287333 #Duplication Level Percentage of deduplicated Percentage of total 1 90.48419208328366 47.16640801115795 2 4.5156813651728935 4.7077498248995395 3 1.5078267486512538 2.357940210315304 4 0.7278765932221597 1.5176716987687184 5 0.5175811910066764 1.3489895346278953 6 0.3154733182296643 0.9866746600704736 7 0.2649831181165225 0.9668883706840561 8 0.17806562499473666 0.7425576315945425 9 0.15165936262804058 0.7114949573576455 >10 1.0780150824387036 10.887457759666788 >50 0.1327992180807441 4.859111818515392 >100 0.11660724729993745 12.530986024939637 >500 0.006890082245589409 2.3769515271745685 >1k 0.0022081989977181023 1.6328530694030705 >5k 7.038281599897812E-5 0.2014550567216864 >10k+ 7.03828156041128E-5 7.004809844102845 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 190928 6.964083148223124 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 5491 0.200283774862216 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004778214260963448 0.0 0.0 2 0.005142963441189666 0.0 0.0 3 0.006164261145823075 0.0 0.0 4 0.006820809670230266 0.0 0.0 5 0.008498655899270867 0.0 0.0 6 0.010176502128311466 0.0 0.0 7 0.013094495570121207 0.0 0.0 8 0.015647739831704727 0.0 0.0 9 0.01838335868340136 0.0 0.0 10-11 0.03098544286021717 0.0 0.0 12-13 0.041836730971947136 0.0 0.0 14-15 0.05321690539500513 0.0 0.0 16-17 0.06266390916286416 0.0 0.0 18-19 0.07626905358530206 0.0 0.0 20-21 0.08485889677962949 0.0 0.0 22-23 0.09251862956438006 0.0 0.0 24-25 0.10057958644737947 0.0 0.0 26-27 0.10856759349433362 0.0 0.0 28-29 0.11690211226250269 0.0 0.0 30-31 0.22094681592203122 0.0 0.0 32-33 0.2339865991151185 0.0 1.8237459011310872E-5 34-35 0.24425428853848652 0.0 3.6474918022621744E-5 36-37 0.2559809746827594 0.0 7.294983604524349E-5 38-39 0.268747195990677 0.0 7.294983604524349E-5 40-41 0.28426727360930254 0.0 7.294983604524349E-5 42-43 0.3018846590142289 0.0 7.294983604524349E-5 44-45 0.32083337892698083 0.0 7.294983604524349E-5 46-47 0.34215296851120325 0.0 7.294983604524349E-5 48-49 0.3669194378485634 0.0 7.294983604524349E-5 50-51 0.3927436798085796 0.0 7.294983604524349E-5 52-53 0.4230178617673557 0.0 7.294983604524349E-5 54-55 0.4576690338888464 3.6474918022621744E-5 9.118729505655436E-5 56-57 0.4952017245341241 3.6474918022621744E-5 1.0942475406786524E-4 58-59 0.5373120173912409 3.6474918022621744E-5 1.0942475406786524E-4 60-61 0.5838904877061288 3.6474918022621744E-5 1.0942475406786524E-4 62-63 0.6315084931846615 3.6474918022621744E-5 1.0942475406786524E-4 64-65 0.6810961442364158 3.6474918022621744E-5 1.0942475406786524E-4 66-67 0.7350243105328621 3.6474918022621744E-5 1.0942475406786524E-4 68-69 0.7912503966647335 3.6474918022621744E-5 1.0942475406786524E-4 70-71 0.8515434361561273 3.6474918022621744E-5 1.0942475406786524E-4 72-73 0.9168335394166202 3.6474918022621744E-5 1.0942475406786524E-4 74-75 0.9885249907900833 3.6474918022621744E-5 1.0942475406786524E-4 76-77 1.0651770310146227 3.6474918022621744E-5 1.2766221307917611E-4 78-79 1.1478291952538835 3.6474918022621744E-5 1.4589967209048698E-4 80-81 1.2401107378511167 3.6474918022621744E-5 1.4589967209048698E-4 82-83 1.3385383041351613 3.6474918022621744E-5 1.4589967209048698E-4 84-85 1.4495497171370109 3.6474918022621744E-5 1.4589967209048698E-4 86-87 1.5645004212853033 3.6474918022621744E-5 1.4589967209048698E-4 88-89 1.6837369283012538 3.6474918022621744E-5 1.8237459011310873E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCGG 30 1.3692988E-6 79.1526 2 TCGGAAG 27705 0.0 75.64571 3 CGGAAGA 27685 0.0 75.52744 4 GATCGGA 27915 0.0 75.18248 1 ATCGGAA 28045 0.0 74.69613 2 GAAGAGC 28270 0.0 74.13252 6 CGTACCG 20 0.00206072 71.36881 1 GGAAGAG 30000 0.0 69.92086 5 AGAGCAC 34600 0.0 60.474045 8 GAGCACA 34630 0.0 60.35309 9 AAGAGCA 35135 0.0 59.58024 7 CGGGTCG 35 2.7657728E-4 54.37624 1 CGACGGG 95 7.2759576E-12 50.083378 1 ATGCCGT 23265 0.0 44.84765 48-49 GCCGTCT 23330 0.0 44.826546 50-51 TCGTATG 23505 0.0 44.58491 44-45 CGTCTTC 23510 0.0 44.484154 52-53 TCTCGTA 22870 0.0 44.48132 42-43 TGCCGTC 23305 0.0 44.477154 50-51 TATGCCG 23320 0.0 44.38541 48-49 >>END_MODULE