FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34139-1.341000000356ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34139-1.341000000356ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741610
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1909286.964083148223124TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT54910.200283774862216TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG301.3692988E-679.15262
TCGGAAG277050.075.645713
CGGAAGA276850.075.527444
GATCGGA279150.075.182481
ATCGGAA280450.074.696132
GAAGAGC282700.074.132526
CGTACCG200.0020607271.368811
GGAAGAG300000.069.920865
AGAGCAC346000.060.4740458
GAGCACA346300.060.353099
AAGAGCA351350.059.580247
CGGGTCG352.7657728E-454.376241
CGACGGG957.2759576E-1250.0833781
ATGCCGT232650.044.8476548-49
GCCGTCT233300.044.82654650-51
TCGTATG235050.044.5849144-45
CGTCTTC235100.044.48415452-53
TCTCGTA228700.044.4813242-43
TGCCGTC233050.044.47715450-51
TATGCCG233200.044.3854148-49
Produced by FastQC (version 0.11.2)