##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34098-1.34100000035707.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315508 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.682131091751785 33.0 33.0 33.0 33.0 33.0 2 32.69377000640896 33.0 33.0 33.0 33.0 33.0 3 32.727786731896416 33.0 33.0 33.0 33.0 33.0 4 36.66420932253311 37.0 37.0 37.0 37.0 37.0 5 36.6301031998162 37.0 37.0 37.0 37.0 37.0 6 36.50996239270173 37.0 37.0 37.0 37.0 37.0 7 36.395193624897864 37.0 37.0 37.0 37.0 37.0 8 36.33941968673829 37.0 37.0 37.0 37.0 37.0 9 36.39220464796494 37.0 37.0 37.0 37.0 37.0 10-11 36.305396483190734 37.0 37.0 37.0 37.0 37.0 12-13 36.297966580119784 37.0 37.0 37.0 35.0 37.0 14-15 38.12828977485718 40.0 37.0 40.0 37.0 40.0 16-17 38.14637349557851 40.0 37.0 40.0 37.0 40.0 18-19 38.10453839934908 40.0 37.0 40.0 37.0 40.0 20-21 38.083311091993636 40.0 37.0 40.0 37.0 40.0 22-23 37.88758212020861 40.0 37.0 40.0 33.0 40.0 24-25 37.96831947892212 40.0 37.0 40.0 33.0 40.0 26-27 37.896499385879906 40.0 37.0 40.0 33.0 40.0 28-29 37.711000566614324 40.0 37.0 40.0 33.0 40.0 30-31 37.62090694568967 40.0 37.0 40.0 33.0 40.0 32-33 37.541409487680454 40.0 37.0 40.0 33.0 40.0 34-35 37.4178910200267 40.0 37.0 40.0 33.0 40.0 36-37 37.37107321589906 40.0 37.0 40.0 33.0 40.0 38-39 37.327577360993786 40.0 37.0 40.0 33.0 40.0 40-41 37.256540897289064 40.0 37.0 40.0 33.0 40.0 42-43 36.852984312729646 37.0 37.0 40.0 33.0 40.0 44-45 36.70551170628649 37.0 37.0 40.0 33.0 40.0 46-47 36.866797264358404 37.0 37.0 40.0 33.0 40.0 48-49 36.59759175956205 37.0 37.0 40.0 33.0 40.0 50-51 36.486950379787075 37.0 37.0 40.0 33.0 40.0 52-53 36.40699189983364 37.0 37.0 40.0 33.0 40.0 54-55 36.144641478241496 37.0 37.0 40.0 33.0 40.0 56-57 35.997362781730835 37.0 37.0 40.0 33.0 40.0 58-59 35.803505105575105 37.0 37.0 40.0 27.0 40.0 60-61 35.52102368033278 37.0 33.0 40.0 27.0 40.0 62-63 35.182900901227725 37.0 33.0 37.0 27.0 40.0 64-65 34.86334229896852 37.0 33.0 37.0 27.0 40.0 66-67 34.293021229034835 37.0 33.0 37.0 27.0 40.0 68-69 34.04523197501369 37.0 33.0 37.0 27.0 40.0 70-71 33.66778758700035 37.0 33.0 37.0 27.0 37.0 72-73 32.83155402615755 37.0 33.0 37.0 22.0 37.0 74-75 32.04088195765249 35.0 33.0 37.0 22.0 37.0 76-77 29.40967878323029 33.0 27.0 33.0 18.5 37.0 78-79 31.615488048410974 33.0 33.0 37.0 22.0 37.0 80-81 31.954905575795895 35.0 33.0 37.0 15.0 37.0 82-83 31.890319748409418 37.0 33.0 37.0 15.0 37.0 84-85 31.757667863812173 33.0 33.0 37.0 15.0 37.0 86-87 31.59791587850269 33.0 33.0 37.0 15.0 37.0 88-89 31.47036201127355 33.0 33.0 37.0 15.0 37.0 90-91 31.328789621974963 33.0 33.0 37.0 15.0 37.0 92-93 31.16325791143887 33.0 33.0 37.0 15.0 37.0 94-95 31.08515215667577 33.0 33.0 37.0 15.0 37.0 96-97 30.94770326857001 33.0 33.0 37.0 15.0 37.0 98-99 30.65163022541922 33.0 30.0 37.0 15.0 37.0 100-101 29.118344225111727 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14664554215730874 2306 2 0.1516206298213163 2306 3 0.14572578066371022 2306 4 0.19990326766973965 2306 5 0.2218365220109959 2306 6 0.20385301295860359 2306 7 0.2574460503286602 2306 8 0.29570454072077723 2306 9 0.21935058304725885 2306 10-11 0.25899210154359054 2306 12-13 0.24078415012773036 2306 14-15 0.5201274973376186 2306 16-17 0.5460199956997442 2306 18-19 0.5598280707026078 2306 20-21 0.5728155806822528 2306 22-23 0.6547357248202843 2306 24-25 0.6407408717987906 2306 26-27 0.7051986542711077 2306 28-29 0.6001024258194718 2306 30-31 0.720812965120949 2306 32-33 0.8013688720451384 2306 34-35 0.7589509668603185 2306 36-37 0.7209043053019784 2306 38-39 0.8512078408755812 2306 40-41 0.8786138863842581 2306 42-43 0.7504045065909963 2306 44-45 0.878634501668806 2306 46-47 0.8202426332815804 2306 48-49 0.8855760725164075 2306 50-51 1.0260118447611362 2306 52-53 0.932244912300753 2306 54-55 0.7730882027963304 2306 56-57 0.8797406433949249 2306 58-59 0.8027433259858725 2306 60-61 0.7857939534547498 2306 62-63 0.7493839334791801 2306 64-65 0.7888880142344092 2306 66-67 0.7429810835468871 2306 68-69 0.9118692463559128 2306 70-71 0.8375282065370158 2306 72-73 0.6032586784587508 2306 74-75 0.34934295219387224 2306 76-77 0.39439172209945283 2306 78-79 0.7280516245837951 2306 80-81 0.7328144885021359 2306 82-83 0.8299678521385161 2306 84-85 0.8202637450158043 2306 86-87 0.6396633208500155 2306 88-89 0.6808491013781968 2306 90-91 0.8674728003010408 2306 92-93 0.806126373776241 2306 94-95 0.8828867316776332 2306 96-97 0.9051693823297882 2306 98-99 0.7516702066587584 2306 100-101 0.437623949499919 2307 1 0.11639196495521276 2307 2 0.06817061712909123 2307 3 0.1214114945677025 2307 4 0.1685474713331132 2307 5 0.1445008194631967 2307 6 0.19780149590426532 2307 7 0.18662479687169764 2307 8 0.16287845085985708 2307 9 0.16463108111670977 2307 10-11 0.22452236914903523 2307 12-13 0.218494273175466 2307 14-15 0.47468233440958585 2307 16-17 0.48178132851648314 2307 18-19 0.5303366947323269 2307 20-21 0.5479632112451114 2307 22-23 0.5560490356193597 2307 24-25 0.5163607865605186 2307 26-27 0.5545423328773751 2307 28-29 0.5649586697238647 2307 30-31 0.5604724796101195 2307 32-33 0.5944235554744424 2307 34-35 0.5243322678797 2307 36-37 0.5683060741966841 2307 38-39 0.5996750882669915 2307 40-41 0.6907092383595668 2307 42-43 0.6869247005815637 2307 44-45 0.5615414209329259 2307 46-47 0.743224236242213 2307 48-49 0.6014446879955742 2307 50-51 0.595845443072399 2307 52-53 0.5839910946144684 2307 54-55 0.616537347073475 2307 56-57 0.6763484805069737 2307 58-59 0.6056675467503467 2307 60-61 0.7315871504246516 2307 62-63 0.49923095882353863 2307 64-65 0.37516772405016496 2307 66-67 0.5699296733744035 2307 68-69 0.618375679307583 2307 70-71 0.3824463083152594 2307 72-73 0.21198290991141278 2307 74-75 0.14107196660955879 2307 76-77 0.12431563557859349 2307 78-79 0.40198347404070134 2307 80-81 0.4458721111824673 2307 82-83 0.34975836370180247 2307 84-85 0.44269858048454935 2307 86-87 0.3351191719635871 2307 88-89 0.41497447379466124 2307 90-91 0.39594412256319345 2307 92-93 0.4524386157567619 2307 94-95 0.48712392923537706 2307 96-97 0.42400129725183433 2307 98-99 0.33727088268649297 2307 100-101 0.1555126320474507 2201 1 0.1019753053286152 2201 2 0.0703749642730358 2201 3 0.11387338766320454 2201 4 0.14004664462750327 2201 5 0.15551350366165906 2201 6 0.18953383638628907 2201 7 0.2262028752940779 2201 8 0.2429771727850607 2201 9 0.23298454905621924 2201 10-11 0.17641081820103466 2201 12-13 0.1592354720796365 2201 14-15 0.400924806561072 2201 16-17 0.3381511857438184 2201 18-19 0.386681811134892 2201 20-21 0.4455292571785705 2201 22-23 0.4833695439527972 2201 24-25 0.43153867912499067 2201 26-27 0.5112154160668823 2201 28-29 0.450230336048584 2201 30-31 0.5555523743711035 2201 32-33 0.5739840136021073 2201 34-35 0.6503019875239104 2201 36-37 0.665119033561389 2201 38-39 0.5666548369967614 2201 40-41 0.6162963803718995 2201 42-43 0.7322052373971246 2201 44-45 0.6353004573683165 2201 46-47 0.684226420676012 2201 48-49 0.7436193461497922 2201 50-51 0.7237950321333315 2201 52-53 0.6194392656037451 2201 54-55 0.6951881273146086 2201 56-57 0.68601188460309 2201 58-59 0.6246537160258612 2201 60-61 0.7681924075481987 2201 62-63 0.8209877987564411 2201 64-65 0.7072445050878358 2201 66-67 0.4378797451831389 2201 68-69 0.612217024242959 2201 70-71 0.5972651572031324 2201 72-73 0.5294420875505352 2201 74-75 0.4080299916883021 2201 76-77 0.16561983910506584 2201 78-79 0.49812420709323746 2201 80-81 0.5032810830503394 2201 82-83 0.4558084005113159 2201 84-85 0.47936478451790876 2201 86-87 0.6701772223865952 2201 88-89 0.6245226218534121 2201 90-91 0.6958294699605823 2201 92-93 0.8285646250013805 2201 94-95 0.8097406636878794 2201 96-97 0.5992592656077136 2201 98-99 0.7017318947672564 2201 100-101 0.5283840159647077 1101 1 0.1024466672195814 1101 2 0.08304479963032207 1101 3 0.10119479296763956 1101 4 0.12954748278668404 1101 5 0.09498702363458733 1101 6 0.12999004462204056 1101 7 0.1536717221600412 1101 8 0.19175748967601436 1101 9 0.14631910869690756 1101 10-11 0.15537170885055218 1101 12-13 0.24453253956266252 1101 14-15 0.43997256794794026 1101 16-17 0.42526882694161117 1101 18-19 0.396045270022384 1101 20-21 0.4712978617393375 1101 22-23 0.4281996730106954 1101 24-25 0.3564514415903517 1101 26-27 0.4125406492021426 1101 28-29 0.3955285422456001 1101 30-31 0.40458162070668635 1101 32-33 0.3466759005935032 1101 34-35 0.4429887065057301 1101 36-37 0.48066132966862796 1101 38-39 0.5150300344924617 1101 40-41 0.5091726842815234 1101 42-43 0.5412971020521979 1101 44-45 0.5746798144547824 1101 46-47 0.4851723744639287 1101 48-49 0.5174693818988416 1101 50-51 0.4708987021865809 1101 52-53 0.36104672461825515 1101 54-55 0.47530820689318887 1101 56-57 0.48719215068989286 1101 58-59 0.519475687949992 1101 60-61 0.42976059187970606 1101 62-63 0.4560647331194332 1101 64-65 0.39417836441943166 1101 66-67 0.3582329983466366 1101 68-69 0.5041314433181654 1101 70-71 0.5066766282135546 1101 72-73 0.40172108123395134 1101 74-75 0.183262634238325 1101 76-77 0.15537113959306836 1101 78-79 0.49570662972911705 1101 80-81 0.36397448321741876 1101 82-83 0.45072962668729133 1101 84-85 0.31994920756767087 1101 86-87 0.43176527946682697 1101 88-89 0.4878873464652891 1101 90-91 0.668446957322498 1101 92-93 0.7256484223059161 1101 94-95 0.652862283815093 1101 96-97 0.6860355663420741 1101 98-99 0.5546324948914219 1101 100-101 0.4674650080911569 2304 1 0.040311213760801934 2304 2 0.08787817370838269 2304 3 0.08984226312025356 2304 4 0.12768722369023777 2304 5 0.13189274434375875 2304 6 0.02430573074068576 2304 7 0.05180766355562838 2304 8 0.056198044624153454 2304 9 0.15347668179119722 2304 10-11 0.09407714649637455 2304 12-13 0.1365672710649406 2304 14-15 0.3088540477962951 2304 16-17 0.3317838082515365 2304 18-19 0.24686908833791676 2304 20-21 0.3730293375132234 2304 22-23 0.3939013646416285 2304 24-25 0.3138395848258071 2304 26-27 0.316087014947783 2304 28-29 0.33429109749468466 2304 30-31 0.2013458147435614 2304 32-33 0.20941217624579878 2304 34-35 0.24577274597262289 2304 36-37 0.37382004061556273 2304 38-39 0.44934266600668593 2304 40-41 0.49694976714928885 2304 42-43 0.4133801672952586 2304 44-45 0.4467277592459382 2304 46-47 0.339764454789524 2304 48-49 0.38659383028277006 2304 50-51 0.4445291170478569 2304 52-53 0.38241370759395465 2304 54-55 0.3993515432811279 2304 56-57 0.37186071111131014 2304 58-59 0.45863069237692144 2304 60-61 0.5519120232693453 2304 62-63 0.34463766292501674 2304 64-65 0.4336533774052498 2304 66-67 0.3970619699948017 2304 68-69 0.2974803296373878 2304 70-71 0.35056559459240333 2304 72-73 0.1582396120842091 2304 74-75 0.08495074062854968 2304 76-77 0.05509700009413265 2304 78-79 0.44286397285317847 2304 80-81 0.10799388270518762 2304 82-83 0.14656501600437366 2304 84-85 0.35015004655901905 2304 86-87 0.5058085658612335 2304 88-89 0.5094030601803645 2304 90-91 0.3489107122662318 2304 92-93 0.46391469182401224 2304 94-95 0.4899648790290101 2304 96-97 0.3188897367270087 2304 98-99 0.4002664852896629 2304 100-101 0.2884939348451425 2202 1 0.136981285788913 2202 2 0.10594446387509748 2202 3 0.1157481725002043 2202 4 0.1376101047963303 2202 5 0.18904919312329582 2202 6 0.16705658002893387 2202 7 0.16394409097321727 2202 8 0.049914657947319085 2202 9 0.08425103060499595 2202 10-11 0.2063991849078448 2202 12-13 0.2779516413994898 2202 14-15 0.2613614532491866 2202 16-17 0.19753539238900686 2202 18-19 0.3241305563471144 2202 20-21 0.29041242272466405 2202 22-23 0.31412062726541734 2202 24-25 0.3660276887375815 2202 26-27 0.2861104366847229 2202 28-29 0.2290200904842834 2202 30-31 0.25313312318621684 2202 32-33 0.27283797292891876 2202 34-35 0.2040401910470706 2202 36-37 0.1703409104027216 2202 38-39 0.24886771819755182 2202 40-41 0.1836965207980512 2202 42-43 0.27761061582945956 2202 44-45 0.4504096001387623 2202 46-47 0.22539091433289116 2202 48-49 0.34440547551422895 2202 50-51 0.3196696486202555 2202 52-53 0.36259084469224234 2202 54-55 0.38214127306696355 2202 56-57 0.4504361852823848 2202 58-59 0.34693832746731346 2202 60-61 0.4136458716031868 2202 62-63 0.42539950342200683 2202 64-65 0.287803296314074 2202 66-67 0.21453248684490234 2202 68-69 0.36432077023393106 2202 70-71 0.3538037181929852 2202 72-73 0.05592572306554899 2202 74-75 0.0191643587449164 2202 76-77 0.15586133074728892 2202 78-79 0.45616294878220387 2202 80-81 0.35988146481187755 2202 82-83 0.3642670345268044 2202 84-85 0.32625340783257784 2202 86-87 0.14170665265261206 2202 88-89 0.4014156656178791 2202 90-91 0.28607166726183664 2202 92-93 0.31645337382255434 2202 94-95 0.12565123350251284 2202 96-97 -0.024832135382528264 2202 98-99 0.0999917395928378 2202 100-101 0.23965707022333405 1102 1 0.11296157461356415 1102 2 0.039295734434489304 1102 3 0.07046924686942191 1102 4 0.120825098339985 1102 5 0.14263407839742825 1102 6 0.08476093994007527 1102 7 0.09007167337764344 1102 8 0.08448039876358138 1102 9 0.061163913946998605 1102 10-11 0.08871378619826231 1102 12-13 0.05631924083721884 1102 14-15 0.12287709941833924 1102 16-17 0.2068123985953605 1102 18-19 0.10965924019609474 1102 20-21 0.17552237121163472 1102 22-23 0.15316015236676606 1102 24-25 0.11618260916938539 1102 26-27 0.18604041794597492 1102 28-29 0.21024299264977486 1102 30-31 0.022275939168288517 1102 32-33 0.015282357818016123 1102 34-35 0.09340232139632576 1102 36-37 0.09275188544278734 1102 38-39 0.12018704707694639 1102 40-41 0.18097415833825892 1102 42-43 0.024533445857642278 1102 44-45 0.1542304411474973 1102 46-47 0.042657365823458804 1102 48-49 0.14835162993890805 1102 50-51 -0.014374076546133097 1102 52-53 0.004551877834096274 1102 54-55 -9.665208343037079E-4 1102 56-57 0.17840798287843995 1102 58-59 0.14089150495158265 1102 60-61 0.04646718187517962 1102 62-63 0.19843201393805288 1102 64-65 0.26681684774712977 1102 66-67 0.11705030137045469 1102 68-69 0.12113774591168891 1102 70-71 0.10869925680233905 1102 72-73 -0.11382554622270646 1102 74-75 -0.008226103241049998 1102 76-77 -0.1258321058168974 1102 78-79 0.11904348367876239 1102 80-81 0.03151900290640697 1102 82-83 0.21826218088291682 1102 84-85 0.20075080358040154 1102 86-87 0.07426176460315048 1102 88-89 0.11987847359175063 1102 90-91 0.30864100540997086 1102 92-93 0.26086114346620093 1102 94-95 0.26615479640762274 1102 96-97 0.3210540783409357 1102 98-99 0.08889223353743247 1102 100-101 0.057712900898746256 2305 1 0.022163782616743788 2305 2 -0.008387228228556864 2305 3 0.040926934834196516 2305 4 0.0576316700681474 2305 5 0.09503228943135156 2305 6 -0.0498167946848298 2305 7 0.029824280321044228 2305 8 0.04641877947607753 2305 9 0.007946005810858026 2305 10-11 -0.008781818531041097 2305 12-13 0.0021000266497068765 2305 14-15 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-0.5016387040914907 1213 66-67 -0.4457919184092134 1213 68-69 0.08711964505400971 1213 70-71 0.17636902789212883 1213 72-73 0.4995708875794307 1213 74-75 0.5299437535881637 1213 76-77 -1.9530623850463122 1213 78-79 -1.7436361825840372 1213 80-81 -1.256234455484666 1213 82-83 -0.9967200808950096 1213 84-85 -0.708661487798711 1213 86-87 -0.3902421836271053 1213 88-89 -0.27415687354047336 1213 90-91 -1.4410106957445556 1213 92-93 -0.570234086329517 1213 94-95 -0.1818495130526685 1213 96-97 -0.24625945246481962 1213 98-99 -1.9124405225774765 1213 100-101 -0.495140816397285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 20.0 9 63.0 10 53.0 11 34.0 12 34.0 13 74.0 14 183.0 15 448.0 16 819.0 17 1358.0 18 2107.0 19 2929.0 20 4053.0 21 5654.0 22 7818.0 23 10573.0 24 13958.0 25 18764.0 26 25353.0 27 36466.0 28 59017.0 29 90412.0 30 76680.0 31 57558.0 32 72265.0 33 102585.0 34 157809.0 35 274595.0 36 507718.0 37 659615.0 38 126446.0 39 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.91504099225836 24.245562488536358 20.324736898569682 14.514659620635598 2 29.820827385746956 27.79587667603164 28.741904624108944 13.641391314112461 3 34.76352533179009 18.06203492163578 30.700548916634634 16.473890829939496 4 29.73870096756306 17.49045997681718 28.783705346731693 23.98713370888807 5 36.68135472149056 16.189812337534175 30.085463662719764 17.0433692782555 6 33.184813008635686 17.045546808734844 32.89433679348117 16.8753033891483 7 31.643898444747332 24.54619029603871 25.455493999588857 18.3544172596251 8 28.97688109909359 30.97480120992888 21.939289348168955 18.109028342808575 9 33.54995966327907 23.26664386389509 24.054980591731923 19.12841588109391 10-11 26.033079623269924 29.402302911158866 27.888075339105438 16.676542126465772 12-13 22.23786313845601 28.53786296570774 27.54389101657174 21.680382879264506 14-15 22.155850897513634 28.70862463010277 27.853455915505364 21.28206855687823 16-17 25.33098568435091 25.328739956847485 28.44671666001586 20.893557698785752 18-19 20.749779799797583 25.360326131669247 32.12897480529638 21.76091926323679 20-21 24.147228167641828 25.713558320679525 31.806692095211936 18.33252141646671 22-23 20.344866007804765 32.77660884782087 28.21480210821988 18.663723036154487 24-25 20.631649728698843 24.625265816399683 32.1896750086806 22.553409446220872 26-27 20.387815546307763 24.78792990566217 28.785627171229812 26.038627376800257 28-29 23.525183242726865 29.26444218720039 28.076927395629813 19.13344717444293 30-31 19.278296598413824 26.786109138901704 31.269531351219687 22.666062911464785 32-33 19.02105283160326 30.529542545307553 28.14568120688851 22.303723416200675 34-35 19.535648332892823 30.494431459532855 31.98183724694538 17.988082960628944 36-37 19.130337705592034 31.124789894917228 32.31414013685118 17.43073226263956 38-39 19.4758510666783 27.06378217115395 31.593720767728524 21.866645994439228 40-41 23.315739615276065 26.344906693120222 27.86965000499891 22.4697036866048 42-43 19.882210524179147 30.1177462883618 31.43142241783262 18.56862076962643 44-45 19.40459460556649 26.73219756613912 31.654615983257166 22.208591845037223 46-47 23.103471061816077 26.546028096213142 28.198506435818242 22.151994406152543 48-49 19.602061815598365 30.8531226313846 31.132767708875438 18.412047844141597 50-51 23.09857129252256 27.10970224949246 30.479049661940156 19.312676796044823 52-53 19.240918590474198 26.688799468950947 27.397851013445955 26.672430927128893 54-55 23.38140738019446 26.63341935648291 31.12906301719448 18.85611024612815 56-57 19.898149689851316 26.677969930381483 30.442168386667507 22.981711993099697 58-59 19.75292323774227 26.74536839058862 30.782330555879522 22.71937781578959 60-61 22.89010740069922 27.026091418331475 30.806052689034235 19.277748491935068 62-63 19.32905254315432 27.125906842605918 30.583046213746538 22.961994400493225 64-65 23.40375539201056 27.032853309237826 30.40098374633028 19.162407552421335 66-67 20.287467165139503 33.92199381070575 26.725983436607038 19.064555587547712 68-69 20.53806409248395 33.805048427928355 26.76837539636596 18.88851208322173 70-71 19.974661751665725 34.61606505427851 26.69953654550894 18.709736648546833 72-73 19.856428015070005 34.65347069311046 26.519604094873113 18.97049719694643 74-75 20.589219396299498 33.43368295495759 26.35213320888454 19.624964439858378 76-77 20.678390722365652 32.32818283616909 26.71294424226086 20.2804821992044 78-79 20.477555723293257 31.918246226711545 27.253395878619813 20.35080217137538 80-81 19.810292391397297 31.79111864693724 27.53353764136512 20.865051320300346 82-83 19.070242901341736 31.686653641447148 27.796880857246016 21.446222599965104 84-85 19.225645480786902 31.37141466446315 28.01849712780134 21.38444272694861 86-87 19.18075839720699 31.480829911511037 28.709936816432002 20.628474874849967 88-89 19.477287998968418 31.718028751708776 28.573125660263983 20.231557589058824 90-91 20.22264584877655 31.023632659620237 27.7415492118127 21.01217227979051 92-93 19.62666524963975 31.643636739101368 27.46660428292066 21.263093728338227 94-95 19.486629842464648 31.659849610028072 27.615689080008398 21.237831467498882 96-97 19.906762988250115 32.27161857067687 27.03249082188715 20.789127619185862 98-99 20.175879163596484 31.494938327370104 27.025506129459494 21.303676379573925 100-101 20.169865596147375 31.589290026433233 26.722641992769415 21.518202384649975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 46.0 1 31.5 2 31.5 3 58.5 4 61.5 5 46.0 6 40.5 7 42.0 8 44.0 9 39.5 10 40.0 11 45.0 12 45.5 13 54.0 14 71.0 15 67.0 16 60.0 17 72.0 18 89.0 19 109.5 20 135.5 21 178.5 22 225.5 23 314.5 24 464.5 25 675.0 26 1042.0 27 1689.5 28 2387.5 29 3111.0 30 4105.5 31 5514.5 32 9730.5 33 18577.0 34 33407.0 35 54982.5 36 85891.0 37 125222.0 38 157706.0 39 174183.0 40 181565.5 41 173046.0 42 161186.0 43 160600.5 44 152953.5 45 138134.5 46 127499.5 47 111422.0 48 88265.5 49 71760.0 50 59935.5 51 48594.5 52 37885.0 53 29772.0 54 23255.5 55 16438.5 56 11661.5 57 9118.5 58 7401.0 59 5795.5 60 4354.5 61 3363.0 62 2567.0 63 1933.0 64 1521.5 65 1147.0 66 845.5 67 696.5 68 631.0 69 507.0 70 340.5 71 219.0 72 147.5 73 96.0 74 67.5 75 44.5 76 30.5 77 24.5 78 17.0 79 12.5 80 9.5 81 8.0 82 6.5 83 4.5 84 3.5 85 3.0 86 1.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16635658352292457 2 0.006175750634418019 3 0.0016411085601949983 4 0.0 5 9.069284148446044E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.7274826949421033E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.478060106032888E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.637413474710517E-5 40-41 1.5115473580743405E-4 42-43 9.069284148446044E-4 44-45 4.318706737355258E-4 46-47 0.004621016208970126 48-49 0.003152655918269339 50-51 0.007061085515575848 52-53 0.003930023130993285 54-55 0.018721593706435044 56-57 0.010688799174954265 58-59 0.01891593550961603 60-61 0.00891812941263861 62-63 0.00541997695538085 64-65 0.011962817662474066 66-67 0.030425288964667795 68-69 0.03144018504794628 70-71 0.035650924116867655 72-73 0.030706004902595885 74-75 0.03314607420920161 76-77 0.03368591255137102 78-79 0.01664861447250452 80-81 0.0021593533686776294 82-83 0.0 84-85 0.005247228685886639 86-87 0.025329215014588593 88-89 0.026754388237915826 90-91 0.024875750807166287 92-93 0.03465762156727595 94-95 0.03135381091319918 96-97 0.032908545338647066 98-99 0.03919226364149897 100-101 0.02673279470422905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2315508.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.8295927159029 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20917090940642 28.149323620931753 2 7.175495802690321 4.854882010793687 3 2.7089401420071564 2.749270250875868 4 1.4429140226888848 1.9525277484652022 5 0.8515102114391163 1.4403121823208833 6 0.6768253766683621 1.3738036099486957 7 0.49715177196533067 1.17729093745036 8 0.28818087693870686 0.779923335627855 9 0.2336851915764581 0.7114927369292389 >10 2.2706859511812354 15.330357515696988 >50 0.32944956235812395 7.837038398393576 >100 0.29285671339615005 20.430319240802373 >500 0.01810327112560174 4.024181505307857 >1k 0.004901688134864618 2.6973664575291103 >5k 0.0 0.0 >10k+ 1.2850842307551266E-4 6.491910448926674 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 149329 6.449081583825233 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 4456 0.19244157221655034 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3000 0.12956120212065775 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 2941 0.12701316514561817 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2829 0.12217621359978026 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2521 0.10887459684872608 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2465 0.10645612107580713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004966512747958548 0.0 0.0 2 0.005527944623814731 0.0 0.0 3 0.006218937701791573 0.0 0.0 4 0.0067371825102742035 0.0 0.0 5 0.007903233329360123 0.0 0.0 6 0.009457967754808016 0.0 0.0 7 0.011315011651870778 0.0 0.0 8 0.012653810740450907 0.0 0.0 9 0.014640415839634326 0.0 0.0 10-11 0.022046997894198596 0.0 0.0 12-13 0.028956928673967007 0.0 0.0 14-15 0.037313626210749434 0.0 0.0 16-17 0.044353118192638506 0.0 0.0 18-19 0.05087436536604495 0.0 0.0 20-21 0.058561663358537304 0.0 0.0 22-23 0.06583868421098091 0.0 0.0 24-25 0.07372032400665426 0.0 0.0 26-27 0.08093256425803755 0.0 0.0 28-29 0.08905173292426544 0.0 0.0 30-31 0.1466632808005846 0.0 0.0 32-33 0.15819422778932313 2.1593533686776292E-5 0.0 34-35 0.16864549809372284 4.3187067373552583E-5 0.0 36-37 0.17892402012862837 4.3187067373552583E-5 0.0 38-39 0.1925495398849842 4.3187067373552583E-5 0.0 40-41 0.20706039452249786 4.3187067373552583E-5 0.0 42-43 0.22392494433187016 4.3187067373552583E-5 0.0 44-45 0.24130773894972507 4.3187067373552583E-5 0.0 46-47 0.2627717114343807 4.3187067373552583E-5 0.0 48-49 0.2846891481264586 6.478060106032888E-5 0.0 50-51 0.3112491945611935 8.637413474710517E-5 0.0 52-53 0.34085392924576374 8.637413474710517E-5 0.0 54-55 0.37212136602421586 8.637413474710517E-5 0.0 56-57 0.4065630522546241 8.637413474710517E-5 0.0 58-59 0.4421491957704314 8.637413474710517E-5 0.0 60-61 0.4819676718888468 8.637413474710517E-5 0.0 62-63 0.5264287577499192 8.637413474710517E-5 4.3187067373552583E-5 64-65 0.5716672108237155 8.637413474710517E-5 4.3187067373552583E-5 66-67 0.6201446939505284 8.637413474710517E-5 4.3187067373552583E-5 68-69 0.6723146713377799 8.637413474710517E-5 4.3187067373552583E-5 70-71 0.7288033554623867 8.637413474710517E-5 4.3187067373552583E-5 72-73 0.7860694067997174 8.637413474710517E-5 4.3187067373552583E-5 74-75 0.8466824558584984 8.637413474710517E-5 4.3187067373552583E-5 76-77 0.9113119021830198 8.637413474710517E-5 4.3187067373552583E-5 78-79 0.9842116719095766 8.637413474710517E-5 4.3187067373552583E-5 80-81 1.0619052061145977 8.637413474710517E-5 4.3187067373552583E-5 82-83 1.1466598258351945 8.637413474710517E-5 4.3187067373552583E-5 84-85 1.2389505888124765 8.637413474710517E-5 4.3187067373552583E-5 86-87 1.3338757628995452 8.637413474710517E-5 4.3187067373552583E-5 88-89 1.435538119496888 8.637413474710517E-5 4.3187067373552583E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 21095 0.0 75.86996 4 TCGGAAG 21110 0.0 75.83855 3 GATCGGA 21265 0.0 75.48431 1 GAAGAGC 21230 0.0 75.25003 6 ATCGGAA 21375 0.0 74.85551 2 GGAAGAG 21905 0.0 73.10781 5 ACCGGTG 35 3.392288E-6 67.94386 1 TCGCCGG 30 1.3024636E-4 63.32389 2 GAGCACA 27935 0.0 57.120407 9 AGAGCAC 27995 0.0 57.09977 8 GTTCGCG 25 0.004996596 57.07284 1 AAGAGCA 28070 0.0 56.84569 7 CACGCCG 60 2.329216E-8 55.487484 1 GTTACGG 35 2.7710016E-4 54.355087 1 AACCGAG 165 0.0 51.884407 1 CGTTTGC 55 8.122515E-7 51.884407 1 CGCCGAG 40 5.355492E-4 47.560703 1 ATCGGAC 95 3.8562575E-10 44.99329 2 GCCGTCT 17820 0.0 44.95741 50-51 ATGCCGT 17815 0.0 44.955723 48-49 >>END_MODULE