FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34098-1.34100000035707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34098-1.34100000035707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315508
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1493296.449081583825233TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT44560.19244157221655034TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC30000.12956120212065775No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA29410.12701316514561817No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG28290.12217621359978026No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25210.10887459684872608No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC24650.10645612107580713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA210950.075.869964
TCGGAAG211100.075.838553
GATCGGA212650.075.484311
GAAGAGC212300.075.250036
ATCGGAA213750.074.855512
GGAAGAG219050.073.107815
ACCGGTG353.392288E-667.943861
TCGCCGG301.3024636E-463.323892
GAGCACA279350.057.1204079
AGAGCAC279950.057.099778
GTTCGCG250.00499659657.072841
AAGAGCA280700.056.845697
CACGCCG602.329216E-855.4874841
GTTACGG352.7710016E-454.3550871
AACCGAG1650.051.8844071
CGTTTGC558.122515E-751.8844071
CGCCGAG405.355492E-447.5607031
ATCGGAC953.8562575E-1044.993292
GCCGTCT178200.044.9574150-51
ATGCCGT178150.044.95572348-49
Produced by FastQC (version 0.11.2)