FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34042-1.3410000003584a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34042-1.3410000003584a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5284939
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4356808.243803760081242TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC82960.15697437567396708No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG73990.14000161591269075No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC68650.12989743117186406No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG61950.11721989601015262No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT54600.10331245072081248TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG566650.083.054753
GATCGGA568700.083.040311
CGGAAGA565700.082.8568654
GAAGAGC565800.082.6903466
ATCGGAA573750.082.10382
GGAAGAG579050.081.0045
TGTGCGA255.2816435E-575.986772
TAACGGG750.063.429741
AGTCGCC407.4814525E-659.465381
TGTACCG407.557008E-659.364672
AAGAGCA805750.058.07137
GAGCACA810550.057.8035749
AGAGCAC812600.057.7629438
GTCGCGC250.004992129357.0867651
TCGGACG1750.056.988473
GTCAGCC1200.055.5010221
GGCGCCG1150.053.7773861
GTGCCGA451.50312535E-552.8581161
GATCGGC2850.050.076111
CGGCAGA2800.049.185544
Produced by FastQC (version 0.11.2)