##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34042-1.3410000003584a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5284939 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68966567069175 33.0 33.0 33.0 33.0 33.0 2 32.70386469928981 33.0 33.0 33.0 33.0 33.0 3 32.74061157564922 33.0 33.0 33.0 33.0 33.0 4 36.67237918923946 37.0 37.0 37.0 37.0 37.0 5 36.64434310405475 37.0 37.0 37.0 37.0 37.0 6 36.52809067427268 37.0 37.0 37.0 37.0 37.0 7 36.40921531923074 37.0 37.0 37.0 37.0 37.0 8 36.36306568533714 37.0 37.0 37.0 37.0 37.0 9 36.40038096939246 37.0 37.0 37.0 37.0 37.0 10-11 36.33149446001174 37.0 37.0 37.0 37.0 37.0 12-13 36.32975394796421 37.0 37.0 37.0 37.0 37.0 14-15 38.178909538974814 40.0 37.0 40.0 37.0 40.0 16-17 38.19605685136574 40.0 37.0 40.0 37.0 40.0 18-19 38.16716597864233 40.0 37.0 40.0 37.0 40.0 20-21 38.14314583384974 40.0 37.0 40.0 37.0 40.0 22-23 37.92425153819183 40.0 37.0 40.0 33.0 40.0 24-25 38.02591032744181 40.0 37.0 40.0 35.0 40.0 26-27 37.98248116014206 40.0 37.0 40.0 35.0 40.0 28-29 37.79026115533216 40.0 37.0 40.0 33.0 40.0 30-31 37.72634688877204 40.0 37.0 40.0 33.0 40.0 32-33 37.56310025527257 40.0 37.0 40.0 33.0 40.0 34-35 37.49313766913866 40.0 37.0 40.0 33.0 40.0 36-37 37.51165491219483 40.0 37.0 40.0 33.0 40.0 38-39 37.34379753484383 40.0 37.0 40.0 33.0 40.0 40-41 37.094563816157574 40.0 37.0 40.0 33.0 40.0 42-43 36.9309529400434 37.0 37.0 40.0 33.0 40.0 44-45 36.84114017210038 37.0 37.0 40.0 33.0 40.0 46-47 36.93435193859381 37.0 37.0 40.0 33.0 40.0 48-49 36.642358785976526 37.0 37.0 40.0 33.0 40.0 50-51 36.55087598929713 37.0 37.0 40.0 33.0 40.0 52-53 36.45721814386126 37.0 37.0 40.0 33.0 40.0 54-55 36.17360351746728 37.0 37.0 40.0 33.0 40.0 56-57 36.042703993366814 37.0 37.0 40.0 33.0 40.0 58-59 35.87254942772282 37.0 37.0 40.0 33.0 40.0 60-61 35.580263840320576 37.0 33.0 40.0 27.0 40.0 62-63 35.35252772075515 37.0 33.0 40.0 27.0 40.0 64-65 34.9722115997933 37.0 33.0 37.0 27.0 40.0 66-67 34.26981465632811 37.0 33.0 37.0 27.0 40.0 68-69 34.044997964971785 37.0 33.0 37.0 27.0 38.5 70-71 33.61934168019725 37.0 33.0 37.0 27.0 37.0 72-73 32.65588240469758 37.0 33.0 37.0 22.0 37.0 74-75 31.746632269549373 35.0 33.0 37.0 18.5 37.0 76-77 29.112829589896876 33.0 27.0 33.0 15.0 37.0 78-79 31.228442466412574 33.0 33.0 37.0 15.0 37.0 80-81 31.530652009417707 33.0 33.0 37.0 15.0 37.0 82-83 31.46487490205658 33.0 33.0 37.0 15.0 37.0 84-85 31.32312425933393 33.0 33.0 37.0 15.0 37.0 86-87 31.160956351624876 33.0 33.0 37.0 15.0 37.0 88-89 31.035120272911378 33.0 33.0 37.0 15.0 37.0 90-91 30.883566016561403 33.0 33.0 37.0 15.0 37.0 92-93 30.71505801675289 33.0 33.0 37.0 15.0 37.0 94-95 30.62971285004425 33.0 33.0 37.0 15.0 37.0 96-97 30.49538547180961 33.0 30.0 37.0 15.0 37.0 98-99 30.201363440524098 33.0 27.0 37.0 15.0 37.0 100-101 28.70425883818148 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1462910602658951 2306 2 0.16579858154863558 2306 3 0.14559615948811455 2306 4 0.1766588862918752 2306 5 0.18821980819439688 2306 6 0.19875004209490044 2306 7 0.23232580154206772 2306 8 0.22865352797517602 2306 9 0.2505786715905174 2306 10-11 0.24621674813121786 2306 12-13 0.2662260533035621 2306 14-15 0.5022443249595554 2306 16-17 0.537891564675725 2306 18-19 0.5062142472972866 2306 20-21 0.5987505688109636 2306 22-23 0.5594724750111837 2306 24-25 0.5597823912457898 2306 26-27 0.5867403864305416 2306 28-29 0.5383911308411555 2306 30-31 0.6364709909272293 2306 32-33 0.5770760794709631 2306 34-35 0.6563236917526751 2306 36-37 0.6688858122585586 2306 38-39 0.7099075569721691 2306 40-41 0.6086531331842622 2306 42-43 0.6996374350222041 2306 44-45 0.647785302852661 2306 46-47 0.6664216912760139 2306 48-49 0.7452420706057765 2306 50-51 0.740503466199371 2306 52-53 0.7749313813949144 2306 54-55 0.691936961256701 2306 56-57 0.6850260528841048 2306 58-59 0.7242314781982344 2306 60-61 0.6452438479539566 2306 62-63 0.6416916459477804 2306 64-65 0.5898411121467788 2306 66-67 0.6204997179381095 2306 68-69 0.6197246814401538 2306 70-71 0.7605955955199732 2306 72-73 0.18106022159440016 2306 74-75 0.016999790093809253 2306 76-77 -0.0907593431955398 2306 78-79 0.4007430684273139 2306 80-81 0.6257594469736283 2306 82-83 0.3851849665882199 2306 84-85 0.29209347759147164 2306 86-87 0.25573324327418234 2306 88-89 0.41941685238553816 2306 90-91 0.4685304661659764 2306 92-93 0.419864612802872 2306 94-95 0.4224938199453767 2306 96-97 0.5368441218976905 2306 98-99 0.3706030014524089 2306 100-101 0.12493110800739515 2307 1 0.08948649378650231 2307 2 0.0446804732544166 2307 3 0.11426617668112726 2307 4 0.16646623130375104 2307 5 0.1796596509992625 2307 6 0.1767976271815428 2307 7 0.19965335651480132 2307 8 0.24089825725290126 2307 9 0.2078841904985964 2307 10-11 0.262756534953823 2307 12-13 0.28183075548142966 2307 14-15 0.521923300807849 2307 16-17 0.46138540870180833 2307 18-19 0.47313691427022775 2307 20-21 0.5229804144091403 2307 22-23 0.509931951108193 2307 24-25 0.44403284555186673 2307 26-27 0.5151585697605796 2307 28-29 0.44736796832646064 2307 30-31 0.5506845679778465 2307 32-33 0.5791433816654532 2307 34-35 0.5719078632293417 2307 36-37 0.4989315225287072 2307 38-39 0.5295623175471817 2307 40-41 0.602428820419199 2307 42-43 0.5577461823457028 2307 44-45 0.5821823267806323 2307 46-47 0.49899120894395566 2307 48-49 0.5422476255131201 2307 50-51 0.5273237518599245 2307 52-53 0.571018448829058 2307 54-55 0.5097307639724278 2307 56-57 0.5359541999111457 2307 58-59 0.4551407476023641 2307 60-61 0.5320927554368424 2307 62-63 0.48977781779854723 2307 64-65 0.442320264854537 2307 66-67 0.4556489339790062 2307 68-69 0.5130240381503839 2307 70-71 0.3475087589524861 2307 72-73 0.1065874680921084 2307 74-75 0.1778424401110783 2307 76-77 0.004701405074658993 2307 78-79 0.3694415497695225 2307 80-81 0.5201565406097295 2307 82-83 0.5425203853008327 2307 84-85 0.48827997038174686 2307 86-87 0.4781841791305972 2307 88-89 0.6660857585638844 2307 90-91 0.5828055606329237 2307 92-93 0.6635810066424632 2307 94-95 0.7046800508322839 2307 96-97 0.796224262352915 2307 98-99 0.6247425434999343 2307 100-101 0.4683348443294584 2201 1 0.08467459772033692 2201 2 0.09037210834537035 2201 3 0.09358729084453898 2201 4 0.12301691961631178 2201 5 0.17694231208692202 2201 6 0.14430892121798422 2201 7 0.23915735348883516 2201 8 0.21994341352969116 2201 9 0.2038027365611228 2201 10-11 0.15987667524345284 2201 12-13 0.18359979271219373 2201 14-15 0.3879610901128956 2201 16-17 0.35681503097801226 2201 18-19 0.38469763641154486 2201 20-21 0.3412564061465915 2201 22-23 0.42925338170331173 2201 24-25 0.38147411251499364 2201 26-27 0.49792242413428767 2201 28-29 0.3714516225681592 2201 30-31 0.5540372355339613 2201 32-33 0.6208142367596068 2201 34-35 0.5747334880163919 2201 36-37 0.577930612235626 2201 38-39 0.5072279419515695 2201 40-41 0.569864943527314 2201 42-43 0.5535066965251545 2201 44-45 0.5513037430995311 2201 46-47 0.5726747978388147 2201 48-49 0.5949948046716074 2201 50-51 0.5917902369585732 2201 52-53 0.6372991914574797 2201 54-55 0.6049318563241286 2201 56-57 0.6959731637371078 2201 58-59 0.6946012904078458 2201 60-61 0.7398306513720243 2201 62-63 0.7495874583689144 2201 64-65 0.6860451296903634 2201 66-67 0.7377965559947057 2201 68-69 0.7001002312267062 2201 70-71 0.6794993425335107 2201 72-73 0.3362349852438058 2201 74-75 0.25925741233499977 2201 76-77 0.27876110617179606 2201 78-79 0.49112830739681357 2201 80-81 0.4315670680719528 2201 82-83 0.4509818796091096 2201 84-85 0.4055212672219142 2201 86-87 0.595530487145222 2201 88-89 0.5113952471734464 2201 90-91 0.515538654900876 2201 92-93 0.5326338014335583 2201 94-95 0.6231998789583493 2201 96-97 0.6258086035457708 2201 98-99 0.6390806529897191 2201 100-101 0.5288822491111098 1101 1 0.12432027314582683 1101 2 0.07661751431554364 1101 3 0.09462341068799418 1101 4 0.11054040530493126 1101 5 0.16773179783053394 1101 6 0.11471007969253577 1101 7 0.14488877802759959 1101 8 0.1340252032995508 1101 9 0.14007426319962235 1101 10-11 0.13728802536122942 1101 12-13 0.19530414340737678 1101 14-15 0.39510486377043463 1101 16-17 0.3669287704495545 1101 18-19 0.39058807624693515 1101 20-21 0.4036827412793471 1101 22-23 0.3983902081795847 1101 24-25 0.3992373126176503 1101 26-27 0.40405069952861794 1101 28-29 0.42477650869120964 1101 30-31 0.43275049475180793 1101 32-33 0.4742227548470268 1101 34-35 0.5425603858891606 1101 36-37 0.5603090136507163 1101 38-39 0.6134474105563612 1101 40-41 0.5285227994328849 1101 42-43 0.6695777814035822 1101 44-45 0.6555779093003054 1101 46-47 0.6321618703992797 1101 48-49 0.6915700184486511 1101 50-51 0.7230468244078168 1101 52-53 0.5885000548143111 1101 54-55 0.6548839684322019 1101 56-57 0.7500165973677042 1101 58-59 0.7522596870570695 1101 60-61 0.5375471550518256 1101 62-63 0.5870637435516173 1101 64-65 0.5346841053935592 1101 66-67 0.6004293360586814 1101 68-69 0.6593421361108156 1101 70-71 0.6194275336084871 1101 72-73 0.47320260478023357 1101 74-75 0.22074752772419615 1101 76-77 0.2616862085923124 1101 78-79 0.7226376237685166 1101 80-81 0.5040622334570308 1101 82-83 0.478292642240973 1101 84-85 0.4660011543073068 1101 86-87 0.47824524944713787 1101 88-89 0.5607777454091583 1101 90-91 0.5020943010895458 1101 92-93 0.5436070025443733 1101 94-95 0.5409605586456365 1101 96-97 0.539617730198934 1101 98-99 0.5605704017730488 1101 100-101 0.44899287243274344 2304 1 0.0881533642494503 2304 2 0.08287058553592175 2304 3 0.06309663037216495 2304 4 0.08036701850766548 2304 5 0.10792861965728662 2304 6 0.10124364784392981 2304 7 0.05580366841693518 2304 8 0.09971856781830013 2304 9 0.16723981985173708 2304 10-11 0.14767594816741791 2304 12-13 0.1769374894752076 2304 14-15 0.254874241885652 2304 16-17 0.31006422545228673 2304 18-19 0.33971741626994856 2304 20-21 0.3379059714277446 2304 22-23 0.34730826759655287 2304 24-25 0.3875786818321103 2304 26-27 0.3553474652477675 2304 28-29 0.34576805649918185 2304 30-31 0.35414899816169054 2304 32-33 0.4543602608382429 2304 34-35 0.3910260096333005 2304 36-37 0.4029329373172672 2304 38-39 0.44969907364261275 2304 40-41 0.36361420354825924 2304 42-43 0.4673712932925156 2304 44-45 0.5084436800033174 2304 46-47 0.47400629988599974 2304 48-49 0.4868998702389078 2304 50-51 0.38472424241243175 2304 52-53 0.37336912737411154 2304 54-55 0.36478133069696383 2304 56-57 0.4110814649293033 2304 58-59 0.3674359252049655 2304 60-61 0.41001913693286696 2304 62-63 0.3866238347077413 2304 64-65 0.35247850522961954 2304 66-67 0.2925156464545111 2304 68-69 0.4111799991438758 2304 70-71 0.3410074492123769 2304 72-73 0.1578020934485025 2304 74-75 0.20922571821861524 2304 76-77 0.14015506959325919 2304 78-79 0.39145324747636323 2304 80-81 0.41857787644809363 2304 82-83 0.5436476674531079 2304 84-85 0.3466137603982382 2304 86-87 0.4061825035226825 2304 88-89 0.4462923579460494 2304 90-91 0.44751035270564543 2304 92-93 0.38907459501487196 2304 94-95 0.48887526200959996 2304 96-97 0.17514156698215544 2304 98-99 0.3820844108930146 2304 100-101 0.280686306148457 2202 1 0.07940436900441483 2202 2 0.025510648516196 2202 3 0.03973649805620738 2202 4 0.07029045024859926 2202 5 0.10123348976047453 2202 6 0.04540398715182192 2202 7 0.10206542825663689 2202 8 0.01834828340851402 2202 9 0.08793084852773347 2202 10-11 0.1139571990121766 2202 12-13 0.11017947308908305 2202 14-15 0.0627108341098861 2202 16-17 0.05963151189944682 2202 18-19 0.10182296891690612 2202 20-21 0.17241144992833313 2202 22-23 0.20276908670424376 2202 24-25 0.21515903326601915 2202 26-27 0.15994605701850872 2202 28-29 0.09580758467672013 2202 30-31 0.18309200972885975 2202 32-33 0.1509403424985507 2202 34-35 0.185245434325914 2202 36-37 0.15566656043196048 2202 38-39 0.08393375792197588 2202 40-41 0.02024155903318814 2202 42-43 0.16659374949016126 2202 44-45 0.08148104944034884 2202 46-47 0.15993973035254072 2202 48-49 0.16530203813739064 2202 50-51 0.16923361028708683 2202 52-53 0.25975311837944304 2202 54-55 0.21957582555003086 2202 56-57 0.2980789564209516 2202 58-59 0.22752656141278038 2202 60-61 0.24213049881512205 2202 62-63 0.3156269798597364 2202 64-65 0.2725060784091511 2202 66-67 0.20952456884411674 2202 68-69 0.2363009972951815 2202 70-71 0.25560746984537275 2202 72-73 0.042801798297425364 2202 74-75 0.02071948060047646 2202 76-77 0.030490581895545432 2202 78-79 0.08308742453722218 2202 80-81 0.07886687073319365 2202 82-83 0.22337773937618266 2202 84-85 0.09499812544550679 2202 86-87 0.04171372857620881 2202 88-89 0.17643583447270927 2202 90-91 0.18775613356231347 2202 92-93 0.1212474145241913 2202 94-95 0.18124897182698518 2202 96-97 0.1592934986218566 2202 98-99 0.11750907813085831 2202 100-101 0.05513502185507235 1102 1 0.04394189311474861 1102 2 0.044650187091001214 1102 3 0.05099247032382692 1102 4 0.06038836502916922 1102 5 0.08604189016927677 1102 6 0.08386042142807071 1102 7 0.18631105230205236 1102 8 0.11915007408754974 1102 9 0.09853998892169358 1102 10-11 0.11105701196394335 1102 12-13 0.08779373543028868 1102 14-15 0.13534470857941017 1102 16-17 0.13409594232694388 1102 18-19 0.14495385372971015 1102 20-21 0.20649299267328303 1102 22-23 0.19575569270590876 1102 24-25 0.19545696554990855 1102 26-27 0.19864296422073124 1102 28-29 0.21984103283647727 1102 30-31 0.1984331786838922 1102 32-33 0.12383963460282388 1102 34-35 0.18499264973991814 1102 36-37 0.2696419262341223 1102 38-39 0.13794147520670919 1102 40-41 0.16944439140721812 1102 42-43 0.18123033549156276 1102 44-45 0.2994634897498045 1102 46-47 0.2137426241365361 1102 48-49 0.2730155156473515 1102 50-51 0.1960675902709994 1102 52-53 0.14805432190602374 1102 54-55 0.19402740042890088 1102 56-57 0.32347388134702015 1102 58-59 0.30551482133021324 1102 60-61 0.17137897333122254 1102 62-63 0.3045446563874279 1102 64-65 0.28936761376710507 1102 66-67 0.22274580838600144 1102 68-69 0.31910447600664327 1102 70-71 0.1813503351394914 1102 72-73 0.03153628325717506 1102 74-75 0.0532713366697557 1102 76-77 -0.007344657437688085 1102 78-79 0.23874084982398713 1102 80-81 0.0852650278408511 1102 82-83 0.17625503365897544 1102 84-85 0.20302102612205886 1102 86-87 0.05122742789382784 1102 88-89 0.18552490513026854 1102 90-91 0.31026464171050705 1102 92-93 0.21315546471818791 1102 94-95 0.2565250570948727 1102 96-97 0.20254455809212146 1102 98-99 0.12209052371752449 1102 100-101 0.22894859068887286 2305 1 -0.04953231902410238 2305 2 -0.01827600343890623 2305 3 0.03424938610118744 2305 4 0.02584982531110569 2305 5 0.03694625704907395 2305 6 0.016200155881406886 2305 7 -0.013873482786941338 2305 8 0.010446974299640033 2305 9 -0.06787385362127196 2305 10-11 -0.04117173725720136 2305 12-13 0.011318076380668174 2305 14-15 -0.032467818805315574 2305 16-17 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60-61 -0.6564508479118629 1213 62-63 -0.3970437391058894 1213 64-65 -0.6332807233977533 1213 66-67 -0.6715210431899123 1213 68-69 -0.21678427493585417 1213 70-71 -0.020137830944797486 1213 72-73 0.35629794105343393 1213 74-75 0.42576133943206074 1213 76-77 -2.0555993936739583 1213 78-79 -2.0155376856972715 1213 80-81 -1.443948009079552 1213 82-83 -1.0242239928880323 1213 84-85 -0.6791199160191752 1213 86-87 -0.4593532202320425 1213 88-89 -0.4917121764621122 1213 90-91 -1.6528052338849015 1213 92-93 -0.8300176811334765 1213 94-95 -0.5153411628324989 1213 96-97 -0.3877100773246198 1213 98-99 -2.114115533364796 1213 100-101 -0.7777870537792779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 9.0 9 34.0 10 50.0 11 36.0 12 54.0 13 123.0 14 361.0 15 807.0 16 1614.0 17 2804.0 18 4598.0 19 6369.0 20 9257.0 21 12879.0 22 17626.0 23 23994.0 24 32268.0 25 43090.0 26 59230.0 27 86302.0 28 137608.0 29 225548.0 30 221094.0 31 139557.0 32 157827.0 33 224570.0 34 352324.0 35 613100.0 36 1145736.0 37 1504923.0 38 261113.0 39 33.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.36839231190162 24.5762241219081 20.499757100429257 14.555626465761017 2 27.92730312793425 29.820114166250676 28.53879991083482 13.71378279498026 3 32.81107818462518 18.097606416243302 32.167311877357804 16.92400352177371 4 27.215621599416757 17.745067634650088 28.899292877363393 26.140017888569766 5 37.14906894587757 16.81874861633304 29.056949366777236 16.975233071012152 6 33.087174705327726 17.60107354124617 32.45310873029944 16.858643023126664 7 29.565241907238665 27.092176465991376 25.340103263254317 18.002478363515642 8 26.719097420045905 32.88348266649814 22.618747349780197 17.77867256367576 9 34.80876505859386 22.85769050503705 23.710169597037922 18.623374839331163 10-11 26.300602780607367 30.02849898801771 26.79019350229128 16.880704729083643 12-13 21.65305976095467 28.87152529102039 26.376766127291155 23.098648820733786 14-15 21.268932457776 29.23616802731006 26.81650883922301 22.678390675690927 16-17 25.370898320680713 25.0874324188037 27.523099131323935 22.01857012919165 18-19 19.980565524136708 25.259577311976035 32.180216683715116 22.57964048017214 20-21 24.479204017302756 25.706398881803555 31.796648930101178 18.017748170792512 22-23 19.697370206165104 34.94776382471018 27.166235977368896 18.188629991755818 24-25 19.933153438478666 25.032285140850252 31.914322946773844 23.12023847389724 26-27 20.028916133185266 25.08387514028071 26.89984690457165 27.987361821962374 28-29 24.20769662620515 30.08465187583054 26.77377165564257 18.933879842321737 30-31 18.99636873765241 26.334958643798917 31.166972788143816 23.501699830404853 32-33 18.665352618071847 30.892267630714375 27.196775591922634 23.245604159291148 34-35 19.036681407297227 30.848085474591098 32.36409161960053 17.751141498511146 36-37 18.702938180720036 26.883045152666913 32.405769453826984 22.008247212786074 38-39 24.093677340542765 31.081284361868317 26.793553289656924 18.03148500793199 40-41 19.34968162803153 35.14564844654176 26.703765202766466 18.800904722660235 42-43 19.429296626242735 30.838056491325368 31.477760688093326 18.254886194338567 44-45 19.086197040891765 26.343179808598187 31.612623732166888 22.957999418343157 46-47 24.062438175913776 26.133123442482443 26.905842324702018 22.898596056901766 48-49 19.86228602585775 31.083359832679925 30.866432601982908 18.187921539479422 50-51 24.286259544151513 26.303614387610303 30.579982776105076 18.830143292133116 52-53 19.2670082864285 26.10468604282285 26.460021433468967 28.168284237279686 54-55 24.12292484459666 26.309098856027397 30.94515303672289 18.622823262653053 56-57 19.599886230029938 25.921499856084395 30.87830908908896 23.600304824796705 58-59 19.45459078932365 25.810389913932312 31.43921017009617 23.295809126647864 60-61 23.851863710556522 26.38286767890173 30.878515234666576 18.886753375875177 62-63 19.15033525703544 26.534486419223246 30.80689898690573 23.50827933683558 64-65 24.14584913692406 26.26468545267902 30.88484243372569 18.704622976671224 66-67 19.909578057750004 35.167781601851786 26.102429683941498 18.820210656456716 68-69 19.859179135821467 35.20150619781017 26.13386452282947 18.805450143538895 70-71 19.427804602798826 35.813986855581945 26.333573916198798 18.424634625420428 72-73 19.389280988152606 36.081050272712375 25.930327624889195 18.599341114245824 74-75 20.323479792237308 34.906210185868524 25.614373867979722 19.15593615391444 76-77 20.755571068206937 33.437889150454566 26.193177053886295 19.6133627274522 78-79 20.39837653938166 32.78962176721099 27.02557389533463 19.78642779807272 80-81 19.879316197655086 32.830842101558396 26.84508331627183 20.44475838451469 82-83 19.007135181692732 32.744039240566444 27.23544207416585 21.01338350357497 84-85 19.252355035372364 32.03322122213194 27.553346173074615 21.16107756942108 86-87 19.182740734375788 31.983338134387367 28.030086521176433 20.80383461006041 88-89 19.484767194260563 32.45470415572816 27.660206099557406 20.40032255045387 90-91 19.95869698771717 31.538492257412226 27.413840549894257 21.08897020497635 92-93 19.586623814345558 32.01188202658455 26.89674661995677 21.504747539113122 94-95 19.315370426977864 32.30314604806281 26.824708822808496 21.556774702150836 96-97 19.928556658052894 32.59440406230495 26.552316940040686 20.924722339601466 98-99 20.2470867695108 31.462718921739864 26.685770510446854 21.60442379830248 100-101 20.18050631708705 31.642347999735783 26.28298293923737 21.894162743939795 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 52.0 1 33.0 2 28.5 3 54.0 4 74.5 5 74.5 6 57.0 7 53.0 8 60.0 9 59.0 10 55.5 11 58.5 12 70.0 13 78.0 14 84.5 15 87.0 16 87.0 17 104.0 18 132.5 19 175.5 20 220.5 21 276.0 22 349.5 23 487.0 24 696.5 25 956.5 26 1302.0 27 1807.5 28 2489.0 29 3397.5 30 4954.0 31 8211.5 32 18222.5 33 39544.0 34 74476.0 35 123832.0 36 193219.5 37 286138.0 38 368512.0 39 396514.5 40 401037.0 41 380643.0 42 350015.0 43 360520.0 44 354335.5 45 328022.0 46 328059.5 47 300243.0 48 231436.5 49 179392.0 50 144604.0 51 115956.0 52 87950.0 53 60657.5 54 42735.5 55 30357.0 56 21499.5 57 14537.0 58 8799.0 59 5551.0 60 3766.5 61 2668.5 62 1761.0 63 1009.0 64 638.0 65 446.0 66 329.5 67 251.0 68 200.5 69 141.0 70 88.0 71 63.0 72 44.5 73 36.0 74 26.0 75 16.0 76 12.0 77 7.5 78 6.5 79 4.5 80 3.5 81 5.0 82 3.5 83 1.0 84 0.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17211173109093594 2 0.0069064184089920435 3 0.00193001281566353 4 0.0 5 0.0013055969047135645 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.743645669325606E-4 12-13 0.0 14-15 9.46084713560554E-6 16-17 0.0 18-19 1.892169427121108E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.784338854242216E-5 38-39 2.0813863698332185E-4 40-41 8.514762422044984E-5 42-43 0.0014096662232052253 44-45 6.811809937635987E-4 46-47 0.005439987102973185 48-49 0.004115468503988409 50-51 0.007947111593908653 52-53 0.004039781726903565 54-55 0.019442040863669383 56-57 0.011201643008556958 58-59 0.019233902226686063 60-61 0.009356777817113878 62-63 0.0063766109693981325 64-65 0.012686996008847028 66-67 0.030227406598259694 68-69 0.03220472364960125 70-71 0.03663240010906465 72-73 0.030757214037853604 74-75 0.03398336291109509 76-77 0.03398336291109509 78-79 0.01737011534097177 80-81 0.002592272115155918 82-83 0.0 84-85 0.005771116752719379 86-87 0.02605517301145765 88-89 0.026953953489340175 90-91 0.024692811023930453 92-93 0.03469292644626551 94-95 0.031107265381871013 96-97 0.032762913630601985 98-99 0.0389408468101524 100-101 0.026717432310950043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5284939.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.983082744557542 #Duplication Level Percentage of deduplicated Percentage of total 1 78.45471602993379 16.462217981568656 2 8.466929973986053 3.5532458447304758 3 3.7337328159308036 2.3503567386823763 4 1.6970163625611066 1.4243453901795105 5 1.1354228884207875 1.1912336208898953 6 0.8826168134040049 1.111201297643636 7 0.6711370958000681 0.9857767649880408 8 0.4368598205703766 0.7333332610240617 9 0.364739058368871 0.688801485773342 >10 3.1571124962373123 13.487089929034907 >50 0.44139262402581364 6.4860596008421 >100 0.45485060108764713 20.63072556562168 >500 0.06786313090451115 9.947670874231274 >1k 0.03505997082771338 11.904539614589758 >5k 4.585984437694869E-4 0.6584885891340236 >10k+ 9.171949744308356E-5 8.384913441066068 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 435680 8.243803760081242 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8296 0.15697437567396708 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7399 0.14000161591269075 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 6865 0.12989743117186406 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6195 0.11721989601015262 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 5460 0.10331245072081248 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005525134727193634 0.0 0.0 2 0.005979255389702701 0.0 0.0 3 0.006622592994923877 0.0 0.0 4 0.007076713657432943 0.0 0.0 5 0.008174171925163185 0.0 0.0 6 0.009233786804351005 0.0 0.0 7 0.010596148791878203 0.0 0.0 8 0.011996354167947823 0.0 0.0 9 0.013245185989847754 0.0 0.0 10-11 0.019167676296736824 0.0 0.0 12-13 0.024967175590863016 0.0 0.0 14-15 0.030596379636548314 0.0 0.0 16-17 0.03544033336997835 0.0 0.0 18-19 0.04123983266410454 0.0 0.0 20-21 0.04776781718767237 0.0 9.46084713560554E-6 22-23 0.05457962712530835 0.0 1.892169427121108E-5 24-25 0.061372515368673136 0.0 1.892169427121108E-5 26-27 0.06811809937635988 0.0 1.892169427121108E-5 28-29 0.07608413266453974 0.0 1.892169427121108E-5 30-31 0.11885662256461238 0.0 1.892169427121108E-5 32-33 0.13021909997447464 0.0 1.892169427121108E-5 34-35 0.1403989714923862 0.0 1.892169427121108E-5 36-37 0.15120325892124772 0.0 1.892169427121108E-5 38-39 0.16335098664336523 0.0 1.892169427121108E-5 40-41 0.17606636519361907 0.0 1.892169427121108E-5 42-43 0.18988866285873876 0.0 1.892169427121108E-5 44-45 0.20521523521841972 0.0 1.892169427121108E-5 46-47 0.2208540155335757 0.0 1.892169427121108E-5 48-49 0.2372402027724445 0.0 1.892169427121108E-5 50-51 0.2550171345402473 0.0 1.892169427121108E-5 52-53 0.2741091240598993 0.0 1.892169427121108E-5 54-55 0.29403366812748455 0.0 1.892169427121108E-5 56-57 0.3167207795586666 0.0 1.892169427121108E-5 58-59 0.34119599109847815 0.0 1.892169427121108E-5 60-61 0.36643753125627376 0.0 1.892169427121108E-5 62-63 0.3934766323698344 0.0 1.892169427121108E-5 64-65 0.4213861314198707 0.0 1.892169427121108E-5 66-67 0.45269207459158944 0.0 1.892169427121108E-5 68-69 0.485104936878174 0.0 1.892169427121108E-5 70-71 0.5204790443182031 0.0 1.892169427121108E-5 72-73 0.5592969001155926 0.0 1.892169427121108E-5 74-75 0.6016247302002918 0.0 1.892169427121108E-5 76-77 0.6481626372603355 0.0 1.892169427121108E-5 78-79 0.6983335096204516 0.0 1.892169427121108E-5 80-81 0.7534334833382184 0.0 1.892169427121108E-5 82-83 0.8112865635724462 0.0 1.892169427121108E-5 84-85 0.8790073073691106 0.0 1.892169427121108E-5 86-87 0.9482512475546074 0.0 1.892169427121108E-5 88-89 1.0242029283592489 0.0 1.892169427121108E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 56665 0.0 83.05475 3 GATCGGA 56870 0.0 83.04031 1 CGGAAGA 56570 0.0 82.856865 4 GAAGAGC 56580 0.0 82.690346 6 ATCGGAA 57375 0.0 82.1038 2 GGAAGAG 57905 0.0 81.004 5 TGTGCGA 25 5.2816435E-5 75.98677 2 TAACGGG 75 0.0 63.42974 1 AGTCGCC 40 7.4814525E-6 59.46538 1 TGTACCG 40 7.557008E-6 59.36467 2 AAGAGCA 80575 0.0 58.0713 7 GAGCACA 81055 0.0 57.803574 9 AGAGCAC 81260 0.0 57.762943 8 GTCGCGC 25 0.0049921293 57.086765 1 TCGGACG 175 0.0 56.98847 3 GTCAGCC 120 0.0 55.501022 1 GGCGCCG 115 0.0 53.777386 1 GTGCCGA 45 1.50312535E-5 52.858116 1 GATCGGC 285 0.0 50.07611 1 CGGCAGA 280 0.0 49.18554 4 >>END_MODULE