##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_34012-1.3410000003579b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2838360 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67657344381967 33.0 33.0 33.0 33.0 33.0 2 32.688357713609264 33.0 33.0 33.0 33.0 33.0 3 32.72732317253625 33.0 33.0 33.0 33.0 33.0 4 36.65509590044955 37.0 37.0 37.0 37.0 37.0 5 36.630514452007496 37.0 37.0 37.0 37.0 37.0 6 36.51503755689905 37.0 37.0 37.0 37.0 37.0 7 36.38091574007525 37.0 37.0 37.0 37.0 37.0 8 36.335169252667036 37.0 37.0 37.0 37.0 37.0 9 36.407117138065644 37.0 37.0 37.0 37.0 37.0 10-11 36.3270355064192 37.0 37.0 37.0 37.0 37.0 12-13 36.31519151904621 37.0 37.0 37.0 35.0 37.0 14-15 38.16544818134415 40.0 37.0 40.0 37.0 40.0 16-17 38.19099797065911 40.0 37.0 40.0 37.0 40.0 18-19 38.135698431488606 40.0 37.0 40.0 37.0 40.0 20-21 38.135592032018494 40.0 37.0 40.0 37.0 40.0 22-23 37.9707690356403 40.0 37.0 40.0 33.0 40.0 24-25 38.01010689271269 40.0 37.0 40.0 35.0 40.0 26-27 37.88388840739018 40.0 37.0 40.0 33.0 40.0 28-29 37.70752194929466 40.0 37.0 40.0 33.0 40.0 30-31 37.66659761270593 40.0 37.0 40.0 33.0 40.0 32-33 37.55534833495399 40.0 37.0 40.0 33.0 40.0 34-35 37.51549081159543 40.0 37.0 40.0 33.0 40.0 36-37 37.49245039388943 40.0 37.0 40.0 33.0 40.0 38-39 37.32144918192195 40.0 37.0 40.0 33.0 40.0 40-41 37.24154705534182 40.0 37.0 40.0 33.0 40.0 42-43 36.99960047351287 38.5 37.0 40.0 33.0 40.0 44-45 36.98049525077862 37.0 37.0 40.0 33.0 40.0 46-47 36.98962252850237 37.0 37.0 40.0 33.0 40.0 48-49 36.60375005284742 37.0 37.0 40.0 33.0 40.0 50-51 36.568255260079766 37.0 37.0 40.0 33.0 40.0 52-53 36.54123754562494 37.0 37.0 40.0 33.0 40.0 54-55 36.203837427246725 37.0 37.0 40.0 33.0 40.0 56-57 36.101364168040696 37.0 37.0 40.0 33.0 40.0 58-59 35.80096922166321 37.0 37.0 40.0 33.0 40.0 60-61 35.5266578235319 37.0 35.0 40.0 27.0 40.0 62-63 35.33565879592441 37.0 33.0 38.5 27.0 40.0 64-65 35.06024376752772 37.0 33.0 37.0 27.0 40.0 66-67 34.36396651587536 37.0 33.0 37.0 27.0 40.0 68-69 34.1168990191519 37.0 33.0 37.0 27.0 40.0 70-71 33.706937809157395 37.0 33.0 37.0 27.0 40.0 72-73 32.70684127453882 37.0 33.0 37.0 22.0 38.5 74-75 31.631299588494766 37.0 30.0 37.0 18.5 37.0 76-77 29.123369128651756 33.0 27.0 35.0 15.0 37.0 78-79 31.08883351653772 33.0 33.0 37.0 15.0 37.0 80-81 31.30012471990868 37.0 33.0 37.0 15.0 37.0 82-83 31.194609739426994 37.0 33.0 37.0 15.0 37.0 84-85 31.02060644174805 33.0 33.0 37.0 15.0 37.0 86-87 30.840199446159048 33.0 33.0 37.0 15.0 37.0 88-89 30.69243066418636 33.0 33.0 37.0 15.0 37.0 90-91 30.53124039938556 33.0 33.0 37.0 15.0 37.0 92-93 30.366173071773844 33.0 27.0 37.0 15.0 37.0 94-95 30.27583058526755 33.0 27.0 37.0 15.0 37.0 96-97 30.13982511027495 33.0 27.0 37.0 15.0 37.0 98-99 29.857713080793133 33.0 27.0 37.0 6.0 37.0 100-101 28.405034773601656 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17001087039841423 2306 2 0.21381922329904768 2306 3 0.15033947994879782 2306 4 0.18564600682926624 2306 5 0.23030415160969397 2306 6 0.21314330474864818 2306 7 0.26649994990972914 2306 8 0.2238392047941602 2306 9 0.29729662361586406 2306 10-11 0.2607049244212405 2306 12-13 0.2738918070430785 2306 14-15 0.5988865249945903 2306 16-17 0.6025878279667651 2306 18-19 0.6559210428426923 2306 20-21 0.6711234814078537 2306 22-23 0.7208632242423221 2306 24-25 0.6360417388626445 2306 26-27 0.7066548258956331 2306 28-29 0.6633288375929283 2306 30-31 0.7601307125202936 2306 32-33 0.8503254129431284 2306 34-35 0.7491262421560307 2306 36-37 0.7361727770925768 2306 38-39 0.8350813889145314 2306 40-41 0.8627688720755344 2306 42-43 0.9519045613472841 2306 44-45 0.9281801975852417 2306 46-47 0.7421139651119333 2306 48-49 0.9334522203289239 2306 50-51 0.8476975765618491 2306 52-53 0.8545140263522413 2306 54-55 0.8074480858421467 2306 56-57 0.8820589441709217 2306 58-59 0.7372158843792107 2306 60-61 0.8681718376650238 2306 62-63 0.9237130151100601 2306 64-65 0.9433848545713843 2306 66-67 0.6260146143111385 2306 68-69 0.9525647239340103 2306 70-71 0.7765806707099827 2306 72-73 0.24525113735118254 2306 74-75 0.11485245891353202 2306 76-77 0.10541426273576349 2306 78-79 0.411853415690981 2306 80-81 0.5175522553951772 2306 82-83 0.5063937267687422 2306 84-85 0.42129660528093993 2306 86-87 0.3793554510958437 2306 88-89 0.44023245273443834 2306 90-91 0.5157524952230297 2306 92-93 0.38378576372899786 2306 94-95 0.47020352749236594 2306 96-97 0.5797969360247741 2306 98-99 0.47427557783430885 2306 100-101 0.27603463181025134 2307 1 0.12965286935175158 2307 2 0.0790592012698994 2307 3 0.15764005999488262 2307 4 0.18001268253774327 2307 5 0.21603315473596751 2307 6 0.23627801956160965 2307 7 0.3135283439465155 2307 8 0.22590238241649274 2307 9 0.1631914481361747 2307 10-11 0.24249320348999248 2307 12-13 0.22121981392582057 2307 14-15 0.43052109322056253 2307 16-17 0.4876677384254009 2307 18-19 0.44342059760182906 2307 20-21 0.5018929124037399 2307 22-23 0.45475578008922923 2307 24-25 0.4968882170759841 2307 26-27 0.4805300104993364 2307 28-29 0.5440083984347552 2307 30-31 0.633152815646298 2307 32-33 0.6258473037234822 2307 34-35 0.6173054947541772 2307 36-37 0.6989225572121001 2307 38-39 0.6355189579268625 2307 40-41 0.6786487639025509 2307 42-43 0.5931527701090715 2307 44-45 0.6257801438823449 2307 46-47 0.6777424123036297 2307 48-49 0.7185015804150723 2307 50-51 0.6756106162280133 2307 52-53 0.7297595938082821 2307 54-55 0.7143790474802003 2307 56-57 0.6158521770579171 2307 58-59 0.4969221350128237 2307 60-61 0.6509819787447242 2307 62-63 0.5458645011185368 2307 64-65 0.5287984901752978 2307 66-67 0.5551980288838152 2307 68-69 0.5475572027884894 2307 70-71 0.2585573209095955 2307 72-73 0.014971882287078131 2307 74-75 -0.21335854963235334 2307 76-77 -0.15174863513831482 2307 78-79 0.29880950261295425 2307 80-81 0.30382705531078713 2307 82-83 0.46587475402844447 2307 84-85 0.4897801286293699 2307 86-87 0.4760384752265274 2307 88-89 0.43160299998339724 2307 90-91 0.4807894433641131 2307 92-93 0.36086681948411226 2307 94-95 0.466327192112292 2307 96-97 0.48593947885694533 2307 98-99 0.5108003976145099 2307 100-101 0.20284525512004592 2201 1 0.13476371773784024 2201 2 0.12415600670289706 2201 3 0.08772434229265258 2201 4 0.17007241834342324 2201 5 0.204635749814706 2201 6 0.1503952020241357 2201 7 0.16662914105206283 2201 8 0.1800059315803466 2201 9 0.21525278814851134 2201 10-11 0.27405277420493945 2201 12-13 0.2980947407000869 2201 14-15 0.4368115414726077 2201 16-17 0.49626843270768717 2201 18-19 0.479296696807026 2201 20-21 0.46478539205006086 2201 22-23 0.5286549772485216 2201 24-25 0.44101094625919046 2201 26-27 0.5616581741579054 2201 28-29 0.5568762578511866 2201 30-31 0.6264303868217027 2201 32-33 0.6870017426157489 2201 34-35 0.589935459663181 2201 36-37 0.6046698856508783 2201 38-39 0.6601500436503258 2201 40-41 0.6085462437905704 2201 42-43 0.6031262163103861 2201 44-45 0.6702817214614711 2201 46-47 0.6745813140693215 2201 48-49 0.772469042384806 2201 50-51 0.8176529564689403 2201 52-53 0.7922093599365851 2201 54-55 0.782183406366947 2201 56-57 0.8216721781885639 2201 58-59 0.7730703758339033 2201 60-61 0.8559820565790588 2201 62-63 0.6909989128261245 2201 64-65 0.7756625856870514 2201 66-67 0.8069947731144396 2201 68-69 0.8584322573749361 2201 70-71 0.7995246050893101 2201 72-73 0.39589046675085626 2201 74-75 0.2777912765512838 2201 76-77 0.3630443951654634 2201 78-79 0.6707206059679436 2201 80-81 0.6674848513209177 2201 82-83 0.5679716930087828 2201 84-85 0.5459570616451401 2201 86-87 0.6789474721385496 2201 88-89 0.6547141039938325 2201 90-91 0.8169166197555704 2201 92-93 0.7493302139952327 2201 94-95 0.6605074777397313 2201 96-97 0.6884171096780314 2201 98-99 0.651645932504362 2201 100-101 0.4412639153698876 1101 1 0.10128709365637434 1101 2 0.12549989588372767 1101 3 0.12235102538463138 1101 4 0.12389345710084143 1101 5 0.1520388562662376 1101 6 0.13530660009961792 1101 7 0.15865626004655553 1101 8 0.20902015440751143 1101 9 0.1748155072842863 1101 10-11 0.19981581897540224 1101 12-13 0.2203709960734912 1101 14-15 0.3984245530765733 1101 16-17 0.340196379572383 1101 18-19 0.38468388334056414 1101 20-21 0.41035788022642095 1101 22-23 0.3672978214693643 1101 24-25 0.3343612792163455 1101 26-27 0.4097706990327836 1101 28-29 0.330187566334061 1101 30-31 0.42358065258440547 1101 32-33 0.3764082777076254 1101 34-35 0.45201237147475837 1101 36-37 0.3149863887823088 1101 38-39 0.46131690127560887 1101 40-41 0.5935330202656175 1101 42-43 0.4919280012708853 1101 44-45 0.6036967506221416 1101 46-47 0.47596999955617747 1101 48-49 0.4913269950213248 1101 50-51 0.40033229244984625 1101 52-53 0.5145347189531364 1101 54-55 0.45295640108615487 1101 56-57 0.5183710350212962 1101 58-59 0.6466134190537218 1101 60-61 0.4725515210047675 1101 62-63 0.7043365822121572 1101 64-65 0.4990109372691336 1101 66-67 0.3648229275927761 1101 68-69 0.4652972912736857 1101 70-71 0.34808767742672586 1101 72-73 0.07759117573854724 1101 74-75 0.08327839330596021 1101 76-77 0.13764144377806886 1101 78-79 0.5196299882096547 1101 80-81 0.34895296410423526 1101 82-83 0.3414488339706949 1101 84-85 0.3268514371243292 1101 86-87 0.24215549067733022 1101 88-89 0.42213728263633143 1101 90-91 0.26411297402543354 1101 92-93 0.23245191349816707 1101 94-95 0.3880985563830954 1101 96-97 0.3987314591409046 1101 98-99 0.2439543206126693 1101 100-101 0.32907716646180063 2304 1 0.08962403156022702 2304 2 0.10889546777492853 2304 3 0.06911426534336584 2304 4 0.07686799890911544 2304 5 0.13791669941348772 2304 6 0.08917769752220295 2304 7 0.08640948758711886 2304 8 0.08606984737853907 2304 9 0.1522576158822062 2304 10-11 0.15161883986169045 2304 12-13 0.16661225354855702 2304 14-15 0.3158206972228115 2304 16-17 0.32602625077409897 2304 18-19 0.3077103831114414 2304 20-21 0.35126325995206287 2304 22-23 0.34030691330335117 2304 24-25 0.26392389265977556 2304 26-27 0.2917746243819579 2304 28-29 0.3563858723023685 2304 30-31 0.3664336251323448 2304 32-33 0.36450612275329775 2304 34-35 0.3315321509980862 2304 36-37 0.319972318431752 2304 38-39 0.36298318933597784 2304 40-41 0.2508702654285031 2304 42-43 0.45029908872417224 2304 44-45 0.4265349924597146 2304 46-47 0.35558120696148166 2304 48-49 0.4044021705534888 2304 50-51 0.2630231215260608 2304 52-53 0.35332324924439007 2304 54-55 0.43997474954584703 2304 56-57 0.4145964694230244 2304 58-59 0.37725648340958173 2304 60-61 0.32031174239486404 2304 62-63 0.30970708837838856 2304 64-65 0.35972827037660693 2304 66-67 0.34470470358748173 2304 68-69 0.4552812091688949 2304 70-71 0.4310502577754747 2304 72-73 0.3209878757799416 2304 74-75 0.40119571898332396 2304 76-77 0.17892458340301332 2304 78-79 0.6992203367654604 2304 80-81 0.5513195914446989 2304 82-83 0.8529005809249064 2304 84-85 0.645059576745016 2304 86-87 0.6495552357017509 2304 88-89 0.6941137591710635 2304 90-91 0.7609355782016678 2304 92-93 0.8100608076843265 2304 94-95 0.7995621131270916 2304 96-97 0.7570110846291236 2304 98-99 0.6785361799234799 2304 100-101 0.6596687142310387 2202 1 0.08020837526424174 2202 2 0.07138252907311227 2202 3 0.10046793480392324 2202 4 0.11131864088996224 2202 5 0.11068333081334458 2202 6 0.09590417497407344 2202 7 0.07936104298500624 2202 8 0.10480482087194787 2202 9 0.15521494378079836 2202 10-11 0.1887381062535738 2202 12-13 0.15588825267052187 2202 14-15 0.16886693481229287 2202 16-17 0.16160286773721566 2202 18-19 0.16730783630734436 2202 20-21 0.22560098748845547 2202 22-23 0.23988234658216356 2202 24-25 0.1876649061753355 2202 26-27 0.20781462927109118 2202 28-29 0.25721543980728256 2202 30-31 0.31957736291050765 2202 32-33 0.255615953875882 2202 34-35 0.16146084908186253 2202 36-37 0.2846695304504152 2202 38-39 0.3498933889006963 2202 40-41 0.22750175786392646 2202 42-43 0.23295870997971946 2202 44-45 0.20785080881353935 2202 46-47 0.2573125021527929 2202 48-49 0.35383865743371956 2202 50-51 0.3500687920642349 2202 52-53 0.3665253145412706 2202 54-55 0.4191374968796211 2202 56-57 0.3738426771693426 2202 58-59 0.23393685460067104 2202 60-61 0.27800279545810014 2202 62-63 0.314370120414587 2202 64-65 0.3205528546082803 2202 66-67 0.42499228050382243 2202 68-69 0.3779039673823874 2202 70-71 0.3398383919366168 2202 72-73 0.013579932574195652 2202 74-75 -0.028753360939060002 2202 76-77 0.06418165738375237 2202 78-79 0.19436635247689082 2202 80-81 0.21700393403468965 2202 82-83 0.2644406555578911 2202 84-85 0.32963360024077204 2202 86-87 0.21407309067877378 2202 88-89 0.14358900135642116 2202 90-91 0.17794909438891082 2202 92-93 0.1108702287067409 2202 94-95 0.13209902755521696 2202 96-97 0.026783311698810763 2202 98-99 0.1282574476895313 2202 100-101 0.16510426345254814 1102 1 0.0877180622806435 1102 2 0.08271470141463055 1102 3 0.072099708362245 1102 4 0.07211095782020038 1102 5 0.16588888172053373 1102 6 0.13528038445095092 1102 7 0.12832114399678574 1102 8 0.10997696279199687 1102 9 0.10557495578866849 1102 10-11 0.08773073459634162 1102 12-13 0.05555917812186095 1102 14-15 0.15472553132841682 1102 16-17 0.16600312691522134 1102 18-19 0.22750623495959843 1102 20-21 0.23160231980099866 1102 22-23 0.3230638592063997 1102 24-25 0.2232588107226121 1102 26-27 0.2924945237671963 1102 28-29 0.23671975247897592 1102 30-31 0.2486119190623839 1102 32-33 0.17581696569917682 1102 34-35 0.2530417987758611 1102 36-37 0.22411565092482988 1102 38-39 0.13806154877009646 1102 40-41 0.11823514691985082 1102 42-43 0.2090927835766152 1102 44-45 0.21925156244166288 1102 46-47 0.2402532503782453 1102 48-49 0.24229426611829297 1102 50-51 0.17495337821411283 1102 52-53 0.17765931659393885 1102 54-55 0.25348882992205546 1102 56-57 0.17038310175471594 1102 58-59 0.13437879559128163 1102 60-61 0.0274344524354575 1102 62-63 0.18185430877854714 1102 64-65 0.19726371922823915 1102 66-67 0.07989493193296227 1102 68-69 0.26255378439537225 1102 70-71 0.1394925081073879 1102 72-73 -0.0263440599927236 1102 74-75 -0.287477673850681 1102 76-77 -0.25298749788991515 1102 78-79 0.011388227811576002 1102 80-81 -0.06950121996823455 1102 82-83 0.009593100412107702 1102 84-85 -0.1653923414077525 1102 86-87 -0.16373912147313874 1102 88-89 -0.09185857717493562 1102 90-91 -0.06262466431509495 1102 92-93 0.009671566569377177 1102 94-95 -0.0419646861221743 1102 96-97 -0.17177953094565268 1102 98-99 -0.21749023070022488 1102 100-101 -0.09137348838783765 2305 1 0.009850054149872278 2305 2 -0.009455577753016087 2305 3 0.03268235884907256 2305 4 0.02057682465139976 2305 5 0.0814195105027764 2305 6 0.05248687490870907 2305 7 -0.022958057463711157 2305 8 0.033281925772492116 2305 9 -0.038839683820512505 2305 10-11 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52-53 -0.23115812407743164 1213 54-55 -0.9433351225281754 1213 56-57 -0.9701809609570731 1213 58-59 -1.3918372142785813 1213 60-61 -0.7251930103482067 1213 62-63 -0.5750869225761548 1213 64-65 -0.7463608365713412 1213 66-67 -0.6876358336251585 1213 68-69 -0.10884063848108383 1213 70-71 0.04003944409722493 1213 72-73 0.44774413636653776 1213 74-75 0.4585370750836617 1213 76-77 -2.2074922956420515 1213 78-79 -2.0438506817391726 1213 80-81 -1.5184389923491146 1213 82-83 -1.0806627812586314 1213 84-85 -0.6623404705324383 1213 86-87 -0.6124636272115787 1213 88-89 -0.7544761669181099 1213 90-91 -1.7926638921123619 1213 92-93 -0.9581980808979687 1213 94-95 -0.6605382302784086 1213 96-97 -0.630678340828112 1213 98-99 -2.180942792510887 1213 100-101 -1.0392489444052586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 19.0 10 20.0 11 29.0 12 34.0 13 66.0 14 199.0 15 469.0 16 1023.0 17 1768.0 18 2960.0 19 4007.0 20 5783.0 21 7945.0 22 10716.0 23 14500.0 24 19161.0 25 25794.0 26 35766.0 27 53040.0 28 91315.0 29 142463.0 30 108074.0 31 66746.0 32 80658.0 33 114066.0 34 176026.0 35 298106.0 36 555682.0 37 801068.0 38 220689.0 39 165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41531903299998 22.713616537858734 21.31918051826915 13.551883910872133 2 27.479082228941028 30.502297810147994 29.62372852332357 12.394891437587404 3 33.91975264057804 17.15027204674349 33.80330965835523 15.12666565432324 4 26.785749517326906 17.323947631731 29.9609281416029 25.929374709339196 5 38.720135628953905 16.137189025103478 29.916866666760615 15.225808679181998 6 33.53292041883341 16.986851562169704 34.101241562028775 15.378986456968107 7 29.318514917064785 28.008744486252624 25.973238067052804 16.699502529629786 8 26.316816753336436 35.00690539607379 22.598437125664116 16.07784072492566 9 36.8134415648473 22.784460040304964 23.236587325075043 17.165511069772684 10-11 26.627235404144017 30.920740414260784 26.765854330515857 15.68616985107934 12-13 21.10063557829169 29.342507645259943 26.637389196578305 22.919467579870066 14-15 20.412255886633066 29.90755043804368 27.290403942983243 22.389789732340013 16-17 25.17624614213842 24.973400132470864 28.070840203497795 21.779513521892923 18-19 19.10850264034559 25.0720354486456 33.641052164110995 22.178409746897813 20-21 24.52469031412506 25.60508533096577 33.21446187234882 16.655762482560352 22-23 18.773464254005834 36.66983751180259 27.687167942051044 16.86953029214053 24-25 19.19904451866571 25.099670232105865 33.21957045617892 22.481714793049505 26-27 19.212414915655522 25.401076678081708 27.543969052551475 27.8425393537113 28-29 24.44540156992066 31.133682830930535 27.38891472540481 17.032000873743993 30-31 18.518739694753307 26.293264420299046 32.53028157104807 22.657714313899575 32-33 18.127633563043446 31.885067433306556 27.44558477430629 22.541714229343707 34-35 23.848701362758774 26.23048168660776 27.844829408531684 22.075987542101778 36-37 18.240451613062337 26.272680799010978 33.53016655046102 21.956701037465663 38-39 18.78793462477454 31.56921785817024 27.399071327796204 22.24377618925902 40-41 18.81677385009681 25.86095096866728 38.013256605886816 17.309018575349096 42-43 18.489015630149925 31.308550085023633 32.70527002071818 17.49716426410826 44-45 18.223124233929113 25.968794513144445 32.98005846360576 22.828022789320684 46-47 23.887653462114464 25.995503136582492 27.326540179243857 22.790303222059187 48-49 18.79810192499306 31.741809372273842 32.11397610635308 17.346112596380014 50-51 24.336855435039784 26.18665030845183 31.83261275796296 17.64388149854543 52-53 18.404303071045135 26.034087177600863 26.678145562832334 28.883464188521668 54-55 24.029414236579306 26.253347872716613 32.01308967356689 17.7041482171372 56-57 18.697950745880014 26.082104763928278 32.004219788397485 23.215724701794223 58-59 18.547129020947605 26.005666371249507 32.62228244112066 22.824922166682228 60-61 23.6766155691374 26.285507004328952 32.44606782983839 17.591809596695256 62-63 18.218826181458926 26.389248564322475 32.083459733557135 23.308465520661457 64-65 23.706585351501456 26.267287195151816 32.002589137251405 18.023538316095323 66-67 18.414861567083765 36.927961244668936 26.464025380146243 18.193151808101057 68-69 18.3619860688515 37.2860604460794 26.29707366232657 18.05487982274253 70-71 18.001810854837675 37.64576183135296 26.525040760093805 17.827386553715566 72-73 17.954653584682237 37.12961102946618 26.4761109290253 18.43962445682628 74-75 18.48834416455778 36.29659351691347 26.27165341773459 18.94340890079416 76-77 18.811069192940806 35.254324679949804 26.624542517964912 19.31006360914448 78-79 19.028141067225864 34.460271589964485 26.914712068617398 19.596875274192257 80-81 18.77330846392532 34.3229539726871 26.843352677119665 20.060384886267908 82-83 18.4602023703829 33.8358770557646 27.057755182570215 20.64616539128229 84-85 18.719312404064787 32.94482711358711 27.55889032349445 20.776970158853644 86-87 18.763956465606636 32.76203266929645 27.82655596585658 20.64745489924033 88-89 19.16364297615725 33.10075937625987 27.365315380082833 20.370282267500052 90-91 19.581707209847853 32.52992305085874 27.0385372985109 20.8498324407825 92-93 19.242838891581126 32.57718842239746 27.225040750693864 20.954931935327547 94-95 18.906027751424155 32.62885747756105 27.259455234158807 21.205659536855993 96-97 19.130740938624697 33.306254819505554 26.824821634240585 20.738182607629163 98-99 19.48297978488742 32.433192111328594 26.833349869248785 21.2504782345352 100-101 19.647757862428143 32.396941281406235 26.36304319135211 21.59225766481351 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 147.0 1 108.5 2 95.0 3 170.5 4 180.5 5 122.5 6 106.5 7 102.5 8 92.5 9 101.5 10 101.5 11 99.0 12 106.5 13 99.5 14 105.0 15 126.0 16 140.0 17 175.5 18 242.5 19 309.5 20 441.0 21 643.5 22 995.5 23 1655.0 24 2635.0 25 4126.5 26 6772.0 27 10660.0 28 14930.5 29 19206.5 30 23731.5 31 28290.0 32 35032.5 33 46070.5 34 62406.5 35 86667.0 36 115005.0 37 147609.0 38 184865.5 39 206844.0 40 210160.0 41 200793.5 42 187343.0 43 181234.5 44 168826.0 45 153648.5 46 147067.0 47 130895.5 48 101723.0 49 77616.0 50 62461.0 51 50045.0 52 39431.0 53 31565.5 54 25341.0 55 19650.0 56 14089.5 57 9927.0 58 6990.0 59 5117.0 60 3817.5 61 2780.5 62 1960.5 63 1368.5 64 952.5 65 664.0 66 451.5 67 321.0 68 241.0 69 177.0 70 120.0 71 85.0 72 69.5 73 48.5 74 33.5 75 25.5 76 21.5 77 16.0 78 11.0 79 6.5 80 4.5 81 2.0 82 3.0 83 3.0 84 1.5 85 2.0 86 1.5 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16956975154666779 2 0.007856649614566157 3 0.002149128369903747 4 0.0 5 9.864851533984414E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.0569483786411871E-4 12-13 0.0 14-15 5.2847418932059355E-5 16-17 0.0 18-19 1.7615806310686452E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.5231612621372904E-5 38-39 2.2900548203892387E-4 40-41 5.2847418932059355E-5 42-43 0.0011097957975732464 44-45 4.5801096407784773E-4 46-47 0.005619442213108979 48-49 0.004086867064079257 50-51 0.008261813159711947 52-53 0.003805014163108274 54-55 0.019395002748065784 56-57 0.010675178624275989 58-59 0.019905861131075692 60-61 0.00908975605631421 62-63 0.0053904367310700545 64-65 0.012295832804859144 66-67 0.03052819233641962 68-69 0.031902225228653165 70-71 0.0361476345495286 72-73 0.030651502980594426 74-75 0.03387519553545005 76-77 0.033593342634479065 78-79 0.016875942445637622 80-81 0.002360518045631985 82-83 0.0 84-85 0.006394537690779183 86-87 0.026124240758748012 88-89 0.02705787849321439 90-91 0.024891134316999956 92-93 0.03470313843205231 94-95 0.03114474555729365 96-97 0.03283586296311955 98-99 0.03880762130244225 100-101 0.027005031074282333 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2838360.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.219651666501555 #Duplication Level Percentage of deduplicated Percentage of total 1 90.5049161563119 45.45125363475911 2 4.581930625212359 4.602059199164806 3 1.3705377216428778 2.0648378093011797 4 0.71875101684305 1.4438170280320703 5 0.5106430066729649 1.2822156960525661 6 0.2830491192304063 0.8528776903356641 7 0.29281550437698284 1.0293564842654126 8 0.19762848727108354 0.793986703210516 9 0.15997812236140824 0.7230641021325797 >10 1.1485805507648619 11.207921981587162 >50 0.11535715069829547 3.999333089618996 >100 0.10197909324641584 10.728199579877646 >500 0.011188581602459878 3.734336549318832 >1k 0.0025739877599690844 2.1099023332774385 >5k 0.0 0.0 >10k+ 7.087600507293505E-5 9.976838119065889 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 280298 9.875350554545582 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3231 0.11383334037965585 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2859 0.10072718048450514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004298256739807494 0.0 0.0 2 0.004721036091263969 0.0 0.0 3 0.005143815442720444 0.0 0.0 4 0.005742752857283783 0.0 0.0 5 0.006870164461167717 0.0 0.0 6 0.0077157231640806664 0.0 0.0 7 0.00908975605631421 0.0 0.0 8 0.010499020561169126 0.0 0.0 9 0.011837821840781295 0.0 0.0 10-11 0.019130765653405487 0.0 0.0 12-13 0.02436266012767936 0.0 0.0 14-15 0.030792429431079918 0.0 3.5231612621372904E-5 16-17 0.035760086810693495 0.0 3.5231612621372904E-5 18-19 0.04071012838399639 0.0 3.5231612621372904E-5 20-21 0.04569540156992066 0.0 3.5231612621372904E-5 22-23 0.05235417635536013 0.0 3.5231612621372904E-5 24-25 0.059259572429149227 0.0 3.5231612621372904E-5 26-27 0.06611212108400626 0.0 3.5231612621372904E-5 28-29 0.07458532391944644 0.0 3.5231612621372904E-5 30-31 0.14126115080539464 0.0 3.5231612621372904E-5 32-33 0.15271142490734085 0.0 7.046322524274581E-5 34-35 0.16160740709423752 0.0 7.046322524274581E-5 36-37 0.17023915218647387 0.0 7.046322524274581E-5 38-39 0.17989261404473006 0.0 7.046322524274581E-5 40-41 0.19070871911949155 0.0 7.046322524274581E-5 42-43 0.2021589932214377 0.0 7.046322524274581E-5 44-45 0.21422582054425796 0.0 7.046322524274581E-5 46-47 0.22849462365591397 0.0 7.046322524274581E-5 48-49 0.24350329063261883 0.0 7.046322524274581E-5 50-51 0.2614361814568976 0.0 7.046322524274581E-5 52-53 0.278664440028749 0.0 7.046322524274581E-5 54-55 0.29707295762341634 0.0 7.046322524274581E-5 56-57 0.31796530390789046 0.0 7.046322524274581E-5 58-59 0.33959751405741345 0.0 7.046322524274581E-5 60-61 0.3627446835496554 1.7615806310686452E-5 7.046322524274581E-5 62-63 0.38844614495694696 3.5231612621372904E-5 8.807903155343225E-5 64-65 0.41555687086909343 3.5231612621372904E-5 1.0569483786411871E-4 66-67 0.44619075804337716 3.5231612621372904E-5 1.0569483786411871E-4 68-69 0.4780401358530983 3.5231612621372904E-5 1.0569483786411871E-4 70-71 0.5135712171817528 3.5231612621372904E-5 1.0569483786411871E-4 72-73 0.5510576530108936 3.5231612621372904E-5 1.0569483786411871E-4 74-75 0.594181146859454 3.5231612621372904E-5 1.0569483786411871E-4 76-77 0.6397532377852 3.5231612621372904E-5 1.4092645048549161E-4 78-79 0.6872102199861893 3.5231612621372904E-5 1.4092645048549161E-4 80-81 0.7400047914993165 3.5231612621372904E-5 1.4092645048549161E-4 82-83 0.795089417832833 3.5231612621372904E-5 1.4092645048549161E-4 84-85 0.8575374512042164 3.5231612621372904E-5 1.4092645048549161E-4 86-87 0.9276835919333699 3.5231612621372904E-5 1.585422567961781E-4 88-89 1.0025683845600981 3.5231612621372904E-5 1.7615806310686453E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 35090 0.0 84.10444 1 TCGGAAG 35090 0.0 83.80739 3 CGGAAGA 35170 0.0 83.28826 4 ATCGGAA 35435 0.0 83.05236 2 GAAGAGC 35635 0.0 82.161446 6 GGAAGAG 37190 0.0 78.86656 5 TCGGACG 115 0.0 70.20788 3 AGAGCAC 42565 0.0 68.840546 8 GAGCACA 42655 0.0 68.76209 9 AAGAGCA 43570 0.0 67.16545 7 ATCGGAC 140 0.0 57.67583 2 CGCGCGT 25 0.005025781 56.98926 5 CGCGTTG 25 0.005025781 56.98926 6 CGCGCCC 35 2.790511E-4 54.278355 3 GCCGTCT 32245 0.0 45.669056 50-51 CGTCTTC 32350 0.0 45.485317 52-53 ATGCCGT 32245 0.0 45.482502 48-49 TCGTATG 32440 0.0 45.351536 44-45 TCTCGTA 32310 0.0 45.275555 42-43 GTATGCC 32420 0.0 45.249252 46-47 >>END_MODULE