FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_34012-1.3410000003579b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_34012-1.3410000003579b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838360
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2802989.875350554545582TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32310.11383334037965585No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG28590.10072718048450514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA350900.084.104441
TCGGAAG350900.083.807393
CGGAAGA351700.083.288264
ATCGGAA354350.083.052362
GAAGAGC356350.082.1614466
GGAAGAG371900.078.866565
TCGGACG1150.070.207883
AGAGCAC425650.068.8405468
GAGCACA426550.068.762099
AAGAGCA435700.067.165457
ATCGGAC1400.057.675832
CGCGCGT250.00502578156.989265
CGCGTTG250.00502578156.989266
CGCGCCC352.790511E-454.2783553
GCCGTCT322450.045.66905650-51
CGTCTTC323500.045.48531752-53
ATGCCGT322450.045.48250248-49
TCGTATG324400.045.35153644-45
TCTCGTA323100.045.27555542-43
GTATGCC324200.045.24925246-47
Produced by FastQC (version 0.11.2)