##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_30749-1.34100000035870.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4883015 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65967255885964 33.0 33.0 33.0 33.0 33.0 2 32.6419894266145 33.0 33.0 33.0 33.0 33.0 3 32.7053900100655 33.0 33.0 33.0 33.0 33.0 4 36.60781443431978 37.0 37.0 37.0 37.0 37.0 5 36.59962932737253 37.0 37.0 37.0 37.0 37.0 6 36.478564780161435 37.0 37.0 37.0 37.0 37.0 7 36.217726138461586 37.0 37.0 37.0 33.0 37.0 8 36.1959170717272 37.0 37.0 37.0 33.0 37.0 9 36.33316711089358 37.0 37.0 37.0 37.0 37.0 10-11 36.19284714464322 37.0 37.0 37.0 35.0 37.0 12-13 36.253417611864805 37.0 37.0 37.0 35.0 37.0 14-15 38.085418025543646 40.0 37.0 40.0 35.0 40.0 16-17 38.18375163705211 40.0 37.0 40.0 37.0 40.0 18-19 38.028874168930464 40.0 37.0 40.0 37.0 40.0 20-21 38.14051625890971 40.0 37.0 40.0 37.0 40.0 22-23 37.58859741368806 40.0 37.0 40.0 33.0 40.0 24-25 37.97790688744556 40.0 37.0 40.0 35.0 40.0 26-27 38.04954213738848 40.0 37.0 40.0 37.0 40.0 28-29 37.655709843201386 40.0 37.0 40.0 33.0 40.0 30-31 37.55861327479027 40.0 37.0 40.0 33.0 40.0 32-33 37.294466943066936 40.0 37.0 40.0 33.0 40.0 34-35 37.259196111418866 40.0 37.0 40.0 33.0 40.0 36-37 37.48506220030043 40.0 37.0 40.0 33.0 40.0 38-39 37.140800714312775 40.0 37.0 40.0 33.0 40.0 40-41 36.55539569302982 37.0 37.0 40.0 33.0 40.0 42-43 36.32904701296228 37.0 37.0 40.0 33.0 40.0 44-45 36.28543850469433 37.0 37.0 40.0 33.0 40.0 46-47 36.798618271703035 37.0 37.0 40.0 33.0 40.0 48-49 36.3077407093773 37.0 37.0 40.0 33.0 40.0 50-51 36.48206323347358 37.0 37.0 40.0 33.0 40.0 52-53 36.499569733044034 37.0 37.0 40.0 33.0 40.0 54-55 35.91844782373185 37.0 35.0 40.0 30.0 40.0 56-57 35.92191750383728 37.0 37.0 40.0 33.0 40.0 58-59 35.93866361254266 37.0 37.0 40.0 30.0 40.0 60-61 35.6311055771895 37.0 35.0 40.0 27.0 40.0 62-63 35.491821651172486 37.0 33.0 40.0 27.0 40.0 64-65 35.015290860257444 37.0 33.0 38.5 27.0 40.0 66-67 33.5224459683208 37.0 33.0 37.0 27.0 40.0 68-69 33.430008509087116 37.0 33.0 37.0 27.0 38.5 70-71 32.73177995562168 35.0 33.0 37.0 24.5 37.0 72-73 30.37186031580898 33.0 27.0 37.0 15.0 37.0 74-75 28.38549431037996 33.0 22.0 37.0 10.5 37.0 76-77 25.938151633775444 30.0 22.0 33.0 6.0 35.0 78-79 27.159099961806383 33.0 18.5 37.0 6.0 37.0 80-81 27.052136026614704 33.0 15.0 37.0 6.0 37.0 82-83 26.87381996573838 33.0 15.0 37.0 6.0 37.0 84-85 26.64932423922515 33.0 15.0 37.0 6.0 37.0 86-87 26.447643003349363 33.0 15.0 37.0 6.0 37.0 88-89 26.284329456288788 33.0 15.0 37.0 6.0 37.0 90-91 26.10749557394356 33.0 15.0 37.0 6.0 37.0 92-93 25.954911975490553 33.0 15.0 37.0 6.0 37.0 94-95 25.87219484273548 33.0 15.0 37.0 6.0 37.0 96-97 25.742388360469917 33.0 15.0 37.0 6.0 37.0 98-99 25.482092621054818 33.0 15.0 37.0 6.0 37.0 100-101 24.247396434375073 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1415151963303316 2306 2 0.19435789688842675 2306 3 0.19168593209128915 2306 4 0.21274534211978846 2306 5 0.16470984405508204 2306 6 0.24629026721160585 2306 7 0.27982664502729193 2306 8 0.28671711194594707 2306 9 0.2704491656946715 2306 10-11 0.2609301402909665 2306 12-13 0.23666777744875134 2306 14-15 0.49163512594329717 2306 16-17 0.5726605042482689 2306 18-19 0.5689601730693994 2306 20-21 0.6148967926957312 2306 22-23 0.6112985313521335 2306 24-25 0.5855740288147615 2306 26-27 0.6110812995864308 2306 28-29 0.5642989774293241 2306 30-31 0.6874189391997163 2306 32-33 0.551948497976106 2306 34-35 0.6739428180571068 2306 36-37 0.6126801784368112 2306 38-39 0.6919500249567747 2306 40-41 0.7198913343084783 2306 42-43 0.7223436910345384 2306 44-45 0.84498743965964 2306 46-47 0.7352490951120458 2306 48-49 0.8558248229872802 2306 50-51 0.7596464399575424 2306 52-53 0.8100748130571276 2306 54-55 0.8276872280325591 2306 56-57 0.8126332670639513 2306 58-59 0.7501889049809876 2306 60-61 0.7191951586705514 2306 62-63 0.6983445084285549 2306 64-65 0.5948101265973733 2306 66-67 0.6279317569027683 2306 68-69 0.767913701228359 2306 70-71 0.45955058197696275 2306 72-73 -0.8264985071714257 2306 74-75 -0.6644126264656478 2306 76-77 -0.35280244961964513 2306 78-79 0.09050593298669796 2306 80-81 0.23091335894022436 2306 82-83 0.24979819390006952 2306 84-85 0.21750810811776589 2306 86-87 0.10528503854108706 2306 88-89 0.23705857575757605 2306 90-91 0.08592331312490487 2306 92-93 -0.035390983128305464 2306 94-95 0.043966139497193524 2306 96-97 0.15945433915692675 2306 98-99 0.15944224858311173 2306 100-101 0.05555151792983892 2307 1 0.19045243924554 2307 2 0.15447180347261735 2307 3 0.13227337524641314 2307 4 0.17826334919297437 2307 5 0.2405122484523048 2307 6 0.26879145789012426 2307 7 0.31859716827771223 2307 8 0.3536926006223311 2307 9 0.2888174516936104 2307 10-11 0.3585285329437653 2307 12-13 0.2963705191835331 2307 14-15 0.5360546228378524 2307 16-17 0.5513627552878049 2307 18-19 0.5288214726630969 2307 20-21 0.5341308791907551 2307 22-23 0.5882582970937875 2307 24-25 0.5480075640913356 2307 26-27 0.5530170396703511 2307 28-29 0.5638659775429105 2307 30-31 0.6787903987281751 2307 32-33 0.7421775039580965 2307 34-35 0.7679301738531024 2307 36-37 0.669092908652452 2307 38-39 0.7546940144144685 2307 40-41 0.5929989288494042 2307 42-43 0.6807829138590549 2307 44-45 0.7114383845113252 2307 46-47 0.6191983881018999 2307 48-49 0.7851997231709831 2307 50-51 0.7305762339082449 2307 52-53 0.6178927052425252 2307 54-55 0.6548175034928008 2307 56-57 0.5790572526196129 2307 58-59 0.6797764144667937 2307 60-61 0.8156647909356991 2307 62-63 0.6867047540855324 2307 64-65 0.558951937998259 2307 66-67 0.6775326862535636 2307 68-69 0.6655921177260424 2307 70-71 0.45125972068485964 2307 72-73 -0.5715249630564401 2307 74-75 -0.6655034293791502 2307 76-77 -0.34090781083768107 2307 78-79 0.29688579677440785 2307 80-81 0.26120959140470035 2307 82-83 0.296694415983179 2307 84-85 0.21821772309707654 2307 86-87 0.27249867241031467 2307 88-89 0.15732553257117488 2307 90-91 0.2557001677769115 2307 92-93 0.2918583839070905 2307 94-95 0.36378945884224834 2307 96-97 0.27013420591480397 2307 98-99 0.19252826050422556 2307 100-101 -0.02315316238804499 2201 1 0.09563385949923742 2201 2 0.08100261526119112 2201 3 0.09848099314309167 2201 4 0.14725184802930613 2201 5 0.1803765022146564 2201 6 0.1520149571617324 2201 7 0.2232028227556384 2201 8 0.24465107095340954 2201 9 0.1989099061428874 2201 10-11 0.28012755449360327 2201 12-13 0.20876590808273932 2201 14-15 0.4211733118106906 2201 16-17 0.3924006260608479 2201 18-19 0.45738255997098776 2201 20-21 0.38967756720813185 2201 22-23 0.4554268254431548 2201 24-25 0.44106175151583926 2201 26-27 0.5044698125962412 2201 28-29 0.4606138400778619 2201 30-31 0.5158179360794009 2201 32-33 0.5379238874105639 2201 34-35 0.5020622002990436 2201 36-37 0.5488067926012903 2201 38-39 0.5942324326067094 2201 40-41 0.5388896825896694 2201 42-43 0.6309441805952147 2201 44-45 0.7107769357309337 2201 46-47 0.529661234999061 2201 48-49 0.6799234855383034 2201 50-51 0.6829304366424651 2201 52-53 0.5872944809134424 2201 54-55 0.6225821677016228 2201 56-57 0.6334627442226477 2201 58-59 0.5967654502156421 2201 60-61 0.6735368419372421 2201 62-63 0.6990235085857108 2201 64-65 0.5672399889929451 2201 66-67 0.5825139221901665 2201 68-69 0.7157222075130818 2201 70-71 0.4376337643094388 2201 72-73 -0.6145160461059156 2201 74-75 -0.7413542020057022 2201 76-77 -0.4409600869050614 2201 78-79 0.13166211824605867 2201 80-81 0.13049982478582223 2201 82-83 0.12348750868078184 2201 84-85 0.09384133712730147 2201 86-87 0.11067804150379601 2201 88-89 0.14867129179850025 2201 90-91 0.01629649232766539 2201 92-93 0.012244291926084117 2201 94-95 0.07358742620808556 2201 96-97 0.15290544644432558 2201 98-99 0.05425681863250986 2201 100-101 -0.059934028746997825 1101 1 0.09346565084888425 1101 2 0.06993405351143167 1101 3 0.11688596056570333 1101 4 0.14833750273606938 1101 5 0.13300924394821578 1101 6 0.16835287833258405 1101 7 0.06361158086122742 1101 8 0.14652393191131807 1101 9 0.15377791053046508 1101 10-11 0.1900432137352226 1101 12-13 0.2669222718177622 1101 14-15 0.37855777931275014 1101 16-17 0.433490001683424 1101 18-19 0.4485163152665166 1101 20-21 0.5072187782887028 1101 22-23 0.481028470398023 1101 24-25 0.4402524523270728 1101 26-27 0.5509725393298481 1101 28-29 0.5434716584584152 1101 30-31 0.48102814844291686 1101 32-33 0.5364597457987088 1101 34-35 0.5853961796247091 1101 36-37 0.5430794892187478 1101 38-39 0.5902120993521933 1101 40-41 0.6342534602494467 1101 42-43 0.6332515359930682 1101 44-45 0.7582226820652522 1101 46-47 0.6971788027604262 1101 48-49 0.7013933635620333 1101 50-51 0.682509508041413 1101 52-53 0.7062839421945952 1101 54-55 0.6422443007941183 1101 56-57 0.7330557690558734 1101 58-59 0.7258920815077801 1101 60-61 0.6023743270632664 1101 62-63 0.763028686860217 1101 64-65 0.5594642439376898 1101 66-67 0.2983900234455845 1101 68-69 0.5623852049433182 1101 70-71 0.4719330499711205 1101 72-73 -0.48813262123546863 1101 74-75 -0.9112961789747871 1101 76-77 -0.42695955977532307 1101 78-79 0.1408709972814144 1101 80-81 -0.14653340376755608 1101 82-83 -0.14973275015542598 1101 84-85 -0.12073942031162233 1101 86-87 -0.08970955389312252 1101 88-89 -0.04781186136757398 1101 90-91 -0.11423265290820694 1101 92-93 -0.13547044561369148 1101 94-95 -0.2561039315773499 1101 96-97 -0.17025725447544104 1101 98-99 -0.10482609189884329 1101 100-101 -0.3250591639208942 2304 1 0.0562936571571484 2304 2 0.08752676811919713 2304 3 0.050558228437338926 2304 4 0.08756322435349517 2304 5 0.13810162203026266 2304 6 0.16946050846885186 2304 7 -0.008081717388414233 2304 8 -0.00399587634695564 2304 9 0.11095426842947376 2304 10-11 0.10957707955852669 2304 12-13 0.19182850512431315 2304 14-15 0.33710092757819865 2304 16-17 0.3239879322273467 2304 18-19 0.35337680941380256 2304 20-21 0.36050362357897114 2304 22-23 0.3219636718387733 2304 24-25 0.24512527394293215 2304 26-27 0.2627391789327618 2304 28-29 0.3455562050555372 2304 30-31 0.3435684429702377 2304 32-33 0.45560359602138334 2304 34-35 0.33149389819285346 2304 36-37 0.38085205734218164 2304 38-39 0.4224724469868022 2304 40-41 0.29872739615510824 2304 42-43 0.40826524498829286 2304 44-45 0.48220123793772984 2304 46-47 0.4417530870942059 2304 48-49 0.5123190430960207 2304 50-51 0.48384243329302734 2304 52-53 0.456675870678275 2304 54-55 0.46699608650588686 2304 56-57 0.546511271853646 2304 58-59 0.5830505858316002 2304 60-61 0.621404608710499 2304 62-63 0.5095909526205986 2304 64-65 0.5280534997695057 2304 66-67 0.20736137412522737 2304 68-69 0.4220124619682082 2304 70-71 0.2776212557009856 2304 72-73 -0.24386737378633327 2304 74-75 -0.48525723550969246 2304 76-77 -0.28420414383661097 2304 78-79 0.27651084249374946 2304 80-81 0.09033047405731409 2304 82-83 0.17390977592708978 2304 84-85 0.027753794621336425 2304 86-87 -0.03492415811761873 2304 88-89 0.031074727338481267 2304 90-91 0.12958320871808482 2304 92-93 0.09026597893865684 2304 94-95 0.1292872388378612 2304 96-97 -0.039237801163249486 2304 98-99 -0.03734425366073424 2304 100-101 -0.04593969941479159 2202 1 0.06364468363467779 2202 2 0.035604751029246984 2202 3 0.029089297931243152 2202 4 0.0852658828335251 2202 5 0.09395465435783734 2202 6 0.10380807669145753 2202 7 0.18097926807043763 2202 8 0.05042091915894531 2202 9 0.12283973637434542 2202 10-11 0.14493033225365792 2202 12-13 0.12986382922786532 2202 14-15 0.19167718835657155 2202 16-17 0.16761110914246302 2202 18-19 0.19469467737515345 2202 20-21 0.1993819021779828 2202 22-23 0.27256130710442505 2202 24-25 0.2597904046438302 2202 26-27 0.25636212265469993 2202 28-29 0.23676828104230907 2202 30-31 0.2307188766706929 2202 32-33 0.2059706809588917 2202 34-35 0.11331718906171062 2202 36-37 0.22437282358389155 2202 38-39 0.2333141033735373 2202 40-41 0.0986210211975731 2202 42-43 0.22678459263813266 2202 44-45 0.28640388436944164 2202 46-47 0.32039760507223036 2202 48-49 0.34455442518694923 2202 50-51 0.35395305236652064 2202 52-53 0.4163194582154972 2202 54-55 0.31664866752690557 2202 56-57 0.4846484236476414 2202 58-59 0.4856438495023525 2202 60-61 0.4283095754921362 2202 62-63 0.3619775293990699 2202 64-65 0.27431288676680765 2202 66-67 0.4396950471432959 2202 68-69 0.5253833083117811 2202 70-71 0.5011048587222362 2202 72-73 -0.04914523512500324 2202 74-75 -0.3294126758678111 2202 76-77 0.06385786534031723 2202 78-79 0.3673493041338567 2202 80-81 0.347109011267559 2202 82-83 0.38614569436233026 2202 84-85 0.18991205972623248 2202 86-87 0.2289852323387187 2202 88-89 0.35843792650022976 2202 90-91 0.3000478150452359 2202 92-93 0.29661202052322366 2202 94-95 0.24909062589113162 2202 96-97 0.1920851737288416 2202 98-99 0.24874336323706459 2202 100-101 0.15308232723608128 1102 1 0.09003455021485252 1102 2 0.08982249656871488 1102 3 0.05699105341332 1102 4 0.057394674707296645 1102 5 0.06383906574312448 1102 6 0.02934892612840656 1102 7 0.10676602079035291 1102 8 0.026005431966147796 1102 9 0.10412833759040296 1102 10-11 0.08193459000392522 1102 12-13 0.08748720038246205 1102 14-15 0.08451061450461594 1102 16-17 0.13440100302177171 1102 18-19 0.09390696015618971 1102 20-21 0.1615474775242376 1102 22-23 0.22151098816305392 1102 24-25 0.18133145600994283 1102 26-27 0.1319664003633534 1102 28-29 0.16433651334012467 1102 30-31 0.17860077813043063 1102 32-33 0.07956317196148888 1102 34-35 0.030696500328474485 1102 36-37 0.2011005462650317 1102 38-39 0.10659983091012037 1102 40-41 0.06971976326801155 1102 42-43 0.12282311654768563 1102 44-45 0.15940606677573754 1102 46-47 0.16801329306846213 1102 48-49 0.19069281725621323 1102 50-51 0.1567339288540026 1102 52-53 0.2784543454887185 1102 54-55 0.18060335049518983 1102 56-57 0.22047175345483794 1102 58-59 0.24488946701217174 1102 60-61 0.00998464473378391 1102 62-63 0.2240599408882531 1102 64-65 0.19959880356800141 1102 66-67 0.025407087245817195 1102 68-69 0.15164845842242158 1102 70-71 -0.07213896440892142 1102 72-73 -0.4773624961282934 1102 74-75 -0.5518125242215888 1102 76-77 -0.4391534531292969 1102 78-79 -0.23085628203354602 1102 80-81 -0.34846990685884194 1102 82-83 -0.2719805237941024 1102 84-85 -0.28836677677247735 1102 86-87 -0.3994148026998623 1102 88-89 -0.29207209121489086 1102 90-91 -0.3139949627448537 1102 92-93 -0.28656074863738823 1102 94-95 -0.28428347438369883 1102 96-97 -0.4127573971600782 1102 98-99 -0.39219337113684816 1102 100-101 -0.40575701044096135 2305 1 -0.03694562204759677 2305 2 -0.042913572745604256 2305 3 0.02946274104689195 2305 4 0.005401670069872466 2305 5 0.05696088139730904 2305 6 0.04310498122977435 2305 7 0.00931178314841219 2305 8 0.06088529318363811 2305 9 -0.03692686333811679 2305 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8 10.0 9 41.0 10 47.0 11 29.0 12 41.0 13 98.0 14 274.0 15 695.0 16 1448.0 17 2618.0 18 4383.0 19 6745.0 20 10454.0 21 15646.0 22 23106.0 23 33404.0 24 47334.0 25 67549.0 26 99937.0 27 162229.0 28 298484.0 29 511805.0 30 416858.0 31 147032.0 32 125016.0 33 175479.0 34 266999.0 35 445751.0 36 774421.0 37 981259.0 38 263493.0 39 328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.888860854114284 19.146082646242036 14.373182782491039 12.591873717152646 2 21.4281749891301 46.102799877691844 20.917171863636653 11.5518532695414 3 25.158398229265693 15.368447654346372 46.07347695428129 13.399677162106647 4 21.313102663006358 15.590900294183 20.923753050113504 42.17224399269713 5 52.35483238749291 14.754332362815251 19.449886841155127 13.440948408536709 6 49.117072136784344 15.508246442003557 21.7297509837672 13.644930437444899 7 22.432103935785573 44.38980834586828 18.319460415337655 14.85862730300849 8 20.79213354863747 47.07849556063211 17.816635828478923 14.312735062251496 9 49.29792351651592 16.96507178454295 18.81278267627685 14.924222022664276 10-11 31.254701256583296 34.97202438745174 20.653632610915864 13.119641745049101 12-13 16.66563588274867 34.0462091556139 20.458907048206896 28.829247913430535 14-15 16.517103985366415 34.10581046869823 20.4478422548041 28.929243291131257 16-17 30.895338228532985 19.431099433444295 20.880542042160428 28.79302029586229 18-19 15.997936929937126 19.53458458239112 35.3707976262202 29.096680861451556 20-21 30.5743275414882 19.90592902131163 34.806774093464796 14.712969343735377 22-23 15.925601293463156 49.05431377949894 20.0721582874515 14.947926639586404 24-25 15.815249389977298 19.73420110321185 34.91666521606016 29.533884290750695 26-27 15.794207062644697 19.877012460539238 20.428976769475415 43.89980370734065 28-29 30.35454529629747 34.474807060801574 20.262604149280723 14.908043493620232 30-31 15.561625348273555 20.52692854721929 34.600334014947734 29.311112089559423 32-33 15.381132353679028 34.79409749918852 20.25993162011585 29.564838527016608 34-35 15.477036216354033 34.58136008183469 35.0876456451598 14.853958056651475 36-37 15.34012442518858 20.409165280995992 34.87573096496027 29.374979328855154 38-39 30.005992214217358 34.750418953254794 20.205486255077048 15.038102577450802 40-41 15.491552039693527 49.04077666801215 20.274777068994084 15.192894223300247 42-43 15.342629420470267 34.381446217814414 34.755359490127944 15.520564871587371 44-45 15.309999194141048 20.158874017417816 34.908417253081794 29.622709535359338 46-47 29.974507154534567 20.07073893411336 20.601393581578385 29.353360329773686 48-49 15.391972402894961 34.73843350302314 34.8347300981746 15.03486399590729 50-51 30.042609308918976 20.253292367609717 34.270842581700784 15.433255741770527 52-53 15.733647685845186 20.16059912661502 20.04280909413188 44.062944093407914 54-55 29.76582031712069 20.038991541420472 34.86537359195647 15.329814549502368 56-57 15.485604084468271 20.089796284523327 34.940438251255095 29.484161379753303 58-59 15.569660195144222 20.21251591150209 34.779192031103946 29.438631862249736 60-61 30.03513402778055 20.105370337885326 34.557089953413026 15.302405680921094 62-63 15.523134063183852 20.185592705727426 34.94019233073267 29.351080900356052 64-65 30.02456961695279 20.446412899417567 34.69023664948746 14.838780834142181 66-67 15.479107325231844 48.96342469571281 20.174150264112807 15.383317714942535 68-69 15.477153181637487 49.15264532214924 19.984865172925335 15.385336323287937 70-71 15.476496921327525 48.957463400463425 20.118172756593683 15.447866921615363 72-73 15.418417327496318 48.11424380670656 20.227676385693712 16.239662480103412 74-75 15.649402496541686 46.57563656793891 20.38688048035867 17.388080455160736 76-77 15.987771445756326 44.81536095868325 20.698720971728793 18.498146623831627 78-79 16.20093612090611 42.82151466095445 21.22466686678266 19.752882351356778 80-81 16.62395297876262 40.93470069016363 21.662649245325525 20.778697085748224 82-83 16.819587898050692 39.99868933435592 21.75081379025049 21.43090897734289 84-85 17.122932350383536 38.995166216848894 21.966843057493797 21.915058375273773 86-87 17.421202593195854 38.12285099960045 22.153527953098212 22.30241845410548 88-89 17.853912145719132 37.68832501659251 22.156760322181526 22.30100251550683 90-91 18.19217477391149 36.90664376267328 22.27115995712261 22.630021506292625 92-93 18.405978538213073 36.39213271309216 22.248560688517692 22.95332806017708 94-95 18.66850544591882 36.107255567679566 22.31607316563281 22.908165820768804 96-97 18.795896566668194 36.03156209831781 22.360388857801652 22.81215247721234 98-99 19.075859483357338 35.36855195415816 22.511867129127886 23.04372143335662 100-101 19.17903654975387 36.06621483779626 22.021838429543877 22.73291018290599 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18.0 1 14.0 2 18.5 3 35.0 4 37.0 5 23.0 6 22.0 7 30.0 8 33.0 9 38.5 10 40.0 11 36.0 12 41.0 13 48.0 14 66.0 15 93.0 16 137.5 17 202.5 18 335.0 19 579.0 20 881.5 21 1408.5 22 2208.0 23 3277.0 24 4861.0 25 6849.5 26 9439.0 27 12737.0 28 16538.5 29 21089.0 30 26550.5 31 33043.0 32 43276.5 33 60154.0 34 86413.5 35 124114.0 36 174886.0 37 234954.5 38 289131.0 39 326985.0 40 346292.5 41 344669.0 42 326439.0 43 298501.0 44 264705.0 45 233708.0 46 210322.5 47 192326.5 48 176729.0 49 158853.5 50 139410.0 51 124567.0 52 112386.0 53 99812.0 54 86787.0 55 72765.5 56 59059.0 57 44658.0 58 31917.5 59 23160.5 60 16816.5 61 12295.0 62 8871.0 63 6128.0 64 3966.5 65 2408.0 66 1525.0 67 992.0 68 685.5 69 464.5 70 319.5 71 223.5 72 161.5 73 128.5 74 95.0 75 62.0 76 38.0 77 33.0 78 27.0 79 19.5 80 13.0 81 6.0 82 5.5 83 8.5 84 5.0 85 3.5 86 4.0 87 2.0 88 1.5 89 1.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16622926613987465 2 0.0054474540831842624 3 0.0014130613975177223 4 0.0 5 0.0011468324385651078 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.4574980826395167E-4 12-13 0.0 14-15 2.047915068866264E-5 16-17 0.0 18-19 2.047915068866264E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.023957534433132E-5 38-39 2.4574980826395167E-4 40-41 4.095830137732528E-5 42-43 0.0013209052194187404 44-45 5.4269749324956E-4 46-47 0.005580568562660569 48-49 0.003768163726713926 50-51 0.008119983248054737 52-53 0.0035633722198272996 54-55 0.019055849715800585 56-57 0.01062867920741591 58-59 0.019117287167866575 60-61 0.008734357768714616 62-63 0.0057341621928255395 64-65 0.011509282687028404 66-67 0.030380820046631025 68-69 0.03191675634828073 70-71 0.03627881544496586 72-73 0.031230704800210528 74-75 0.03404658801990164 76-77 0.03385203608835934 78-79 0.017335601057952926 80-81 0.0023551023291962037 82-83 0.0 84-85 0.006082307754532804 86-87 0.025383907278597344 88-89 0.02652050014181812 90-91 0.024329231018131215 92-93 0.03456880636246253 94-95 0.031036152868668232 96-97 0.032572089170317925 98-99 0.038603199048129075 100-101 0.026612656319917098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4883015.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.26853591334163 #Duplication Level Percentage of deduplicated Percentage of total 1 90.62783872809227 45.557287658516394 2 4.853656703660533 4.879724326379818 3 1.5758815378294906 2.376517730386613 4 0.7918145385119914 1.5921343026358423 5 0.4967962109409758 1.2486609085649243 6 0.3086634578382105 0.9309636069285776 7 0.186680365830725 0.6568904071854287 8 0.15912010671166535 0.6398987839016081 9 0.1258743955434438 0.5694769415651195 >10 0.7670642247068221 7.148176747292253 >50 0.06793603970201154 2.3878322965726793 >100 0.0361420889926115 3.0989027481385554 >500 0.0011641321092848139 0.3938422946855165 >1k 9.906394097999613E-4 1.046059881191959 >5k 2.9309014593435756E-4 1.023852843074144 >10k+ 8.373997436845996E-5 26.449778522980544 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 1240327 25.400843536216865 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 16348 0.33479315545825683 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT 9589 0.19637457595358607 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT 7916 0.16211295685145344 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT 6829 0.13985212005287717 TruSeq Adapter, Index 13 (97% over 38bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG 6794 0.13913534977877398 TruSeq Adapter, Index 13 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT 6485 0.13280729221597723 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT 5886 0.12054028095346829 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT 5146 0.10538570944385794 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005979912001089491 0.0 0.0 2 0.006369015864174081 0.0 0.0 3 0.007331535946541225 0.0 2.047915068866264E-5 4 0.007823035563069129 0.0 2.047915068866264E-5 5 0.00923609696058685 0.0 2.047915068866264E-5 6 0.010034783837444694 0.0 2.047915068866264E-5 7 0.011693595043226368 0.0 2.047915068866264E-5 8 0.013270489646253391 0.0 2.047915068866264E-5 9 0.014253488879309197 0.0 2.047915068866264E-5 10-11 0.02367389819609401 0.0 2.047915068866264E-5 12-13 0.03047297622473001 0.0 2.047915068866264E-5 14-15 0.03642216949978651 0.0 3.071872603299396E-5 16-17 0.03969883360997253 0.0 5.1197876721656595E-5 18-19 0.04330316413117715 0.0 7.167702741031924E-5 20-21 0.04718396318667872 0.0 8.191660275465056E-5 22-23 0.0511671579956236 0.0 8.191660275465056E-5 24-25 0.05552921709230875 0.0 8.191660275465056E-5 26-27 0.05963528680538561 0.0 8.191660275465056E-5 28-29 0.06485747023099458 0.0 8.191660275465056E-5 30-31 0.11303467222607344 0.0 1.023957534433132E-4 32-33 0.122014779803052 0.0 1.023957534433132E-4 34-35 0.12843499354394775 0.0 1.023957534433132E-4 36-37 0.13509071751776308 0.0 1.023957534433132E-4 38-39 0.1417566810669228 0.0 1.023957534433132E-4 40-41 0.14935444597241665 0.0 1.023957534433132E-4 42-43 0.15797616841234358 0.0 1.2287490413197583E-4 44-45 0.16805191055116564 0.0 1.2287490413197583E-4 46-47 0.1781174131146433 0.0 1.2287490413197583E-4 48-49 0.18901232128101184 0.0 1.4335405482063848E-4 50-51 0.20085950995440316 0.0 1.4335405482063848E-4 52-53 0.21351562507999666 0.0 1.6383320550930112E-4 54-55 0.22710354156192436 0.0 1.8431235619796376E-4 56-57 0.242391227551011 0.0 1.8431235619796376E-4 58-59 0.25933772474587935 0.0 1.8431235619796376E-4 60-61 0.27852668894115623 0.0 1.8431235619796376E-4 62-63 0.29811499657486207 0.0 1.8431235619796376E-4 64-65 0.31863510556490204 0.0 1.8431235619796376E-4 66-67 0.34134648367862885 0.0 1.9455193154229507E-4 68-69 0.3653480482857415 0.0 2.047915068866264E-4 70-71 0.39163303819464 0.0 2.047915068866264E-4 72-73 0.4211127756109699 2.047915068866264E-5 2.047915068866264E-4 74-75 0.4518826995206855 2.047915068866264E-5 2.047915068866264E-4 76-77 0.486297912252983 2.047915068866264E-5 2.047915068866264E-4 78-79 0.5241741014516645 2.047915068866264E-5 2.047915068866264E-4 80-81 0.5643849138288537 2.047915068866264E-5 2.047915068866264E-4 82-83 0.6077699945627855 2.047915068866264E-5 2.047915068866264E-4 84-85 0.6567356438593779 2.047915068866264E-5 2.047915068866264E-4 86-87 0.7086912491565149 2.047915068866264E-5 2.047915068866264E-4 88-89 0.7657768817011621 2.047915068866264E-5 2.047915068866264E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 145895 0.0 91.499596 1 TCGGAAG 146525 0.0 90.81381 3 ATCGGAA 146810 0.0 90.642166 2 CGGAAGA 146975 0.0 90.19973 4 GAAGAGC 147870 0.0 89.71893 6 GAGCACA 149410 0.0 88.68929 9 GGAAGAG 150210 0.0 88.34613 5 AGAGCAC 150825 0.0 87.85094 8 AAGAGCA 151045 0.0 87.65696 7 GCGCACA 800 0.0 51.051495 9 TCGGACG 640 0.0 48.231293 3 TTGTGTT 24370 0.0 48.135223 1 GCCGTCT 142470 0.0 46.51977 50-51 ATGCCGT 142275 0.0 46.44068 48-49 GTATGCC 142405 0.0 46.40258 46-47 TCTCGTA 140975 0.0 46.30078 42-43 TCGTATG 143335 0.0 46.257256 44-45 AATCTCG 140365 0.0 46.183254 40-41 CGTCTTC 143615 0.0 46.1503 52-53 GTCACAT 142615 0.0 46.067657 28-29 >>END_MODULE