FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_30749-1.34100000035870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_30749-1.34100000035870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883015
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT124032725.400843536216865TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT163480.33479315545825683TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT95890.19637457595358607TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT79160.16211295685145344TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT68290.13985212005287717TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG67940.13913534977877398TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT64850.13280729221597723TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT58860.12054028095346829TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT51460.10538570944385794TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1458950.091.4995961
TCGGAAG1465250.090.813813
ATCGGAA1468100.090.6421662
CGGAAGA1469750.090.199734
GAAGAGC1478700.089.718936
GAGCACA1494100.088.689299
GGAAGAG1502100.088.346135
AGAGCAC1508250.087.850948
AAGAGCA1510450.087.656967
GCGCACA8000.051.0514959
TCGGACG6400.048.2312933
TTGTGTT243700.048.1352231
GCCGTCT1424700.046.5197750-51
ATGCCGT1422750.046.4406848-49
GTATGCC1424050.046.4025846-47
TCTCGTA1409750.046.3007842-43
TCGTATG1433350.046.25725644-45
AATCTCG1403650.046.18325440-41
CGTCTTC1436150.046.150352-53
GTCACAT1426150.046.06765728-29
Produced by FastQC (version 0.11.2)